Search results for the GEO ID: GSE22973  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM567115 | GPL570 |
|
AsPC-1 control untreated cells, biological rep 1
|
Human Pancreatic Cancer Cell Line (ATCC)
|
cell passage: P8
cell line: AsPC-1
|
Human pancreatic cancer cells originally isolated from ascites. Untreated control cells.
|
| Sample_geo_accession | GSM567115
| | Sample_status | Public on Jan 01 2011
| | Sample_submission_date | Jul 16 2010
| | Sample_last_update_date | Jan 01 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | For gene expression studies, cells were serum starved overnight (2.5% foetal calf serum (FCS)) before treatment with a final concentration of PMA (1µM; reconsitituted in DMSO, 0.1%) for 4 hours. In parallel experiments, lysis buffer (350 μl; Qiagen, West Sussex, UK), containing β-mercaptoethanol (0.145 M) to avoid RNA degradation, was added to control and treated cells which were frozen at -80°C for isolation of total RNA
| | Sample_growth_protocol_ch1 | All cell culture reagents were purchased from Sarstedt (Wexford, Ireland). Human pancreatic cancer cell lines, AsPC-1 and BxPC-3, were purchased from the American Tissue Culture Collection (Manassas, VA, USA). Cells were cultured to 80% confluence in RPMI-1640 (Sigma-Aldrich, Ireland) supplemented with L-glutamine (2mM, All chemicals, unless otherwise stated, were purchased from Sigma-Aldrich, Ireland), HEPES (10mM; Invitrogen, NY, USA), sodium pyruvate (1mM), glucose (4.5g/L), sodium bicarbonate (1.5g/L) and foetal calf serum (10%; Invitrogen) in a humidified atmosphere at 37°C and 5% carbon dioxide. At 80% confluency, cells were subcultured by trypsinization using 0.25% trypsin-EDTA. Cells were then counted and resuspended in phosphate buffered saline (PBS) (Sigma-Aldrich, Ireland) at a concentration of 5 x 105/mL. Experiments were performed using three different cell passages.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from cells and tissues with the Qiagen RNeasy Midi Kit (Qiagen, UK), including a DNase digestion step, according to the manufacturer’s instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 3 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human U133 Plus 2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | The probe arrays were scanned at 560nm using a confocal laser-scanning microscope - Affymetrix Scanner 3000.
| | Sample_data_processing | Data was analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Annamarie,,Rogers
| | Sample_contact_email | annamarierogers@gmail.com
| | Sample_contact_phone | 0861911256
| | Sample_contact_laboratory | Molecular Biology
| | Sample_contact_department | Surgery
| | Sample_contact_institute | Trinity College Dublin
| | Sample_contact_address | AMNCH
| | Sample_contact_city | Dublin
| | Sample_contact_zip/postal_code | 24
| | Sample_contact_country | Ireland
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM567nnn/GSM567115/suppl/GSM567115.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM567nnn/GSM567115/suppl/GSM567115.CHP.gz
| | Sample_series_id | GSE22973
| | Sample_data_row_count | 54675
| | |
|
GSM567116 | GPL570 |
|
AsPC-1 control untreated cells, biological rep 2
|
Human Pancreatic Cancer Cell Line (ATCC)
|
cell passage: P10
cell line: AsPC-1
|
Human pancreatic cancer cells originally isolated from ascites. Untreated control cells.
|
| Sample_geo_accession | GSM567116
| | Sample_status | Public on Jan 01 2011
| | Sample_submission_date | Jul 16 2010
| | Sample_last_update_date | Jan 01 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | For gene expression studies, cells were serum starved overnight (2.5% foetal calf serum (FCS)) before treatment with a final concentration of PMA (1µM; reconsitituted in DMSO, 0.1%) for 4 hours. In parallel experiments, lysis buffer (350 μl; Qiagen, West Sussex, UK), containing β-mercaptoethanol (0.145 M) to avoid RNA degradation, was added to control and treated cells which were frozen at -80°C for isolation of total RNA
| | Sample_growth_protocol_ch1 | All cell culture reagents were purchased from Sarstedt (Wexford, Ireland). Human pancreatic cancer cell lines, AsPC-1 and BxPC-3, were purchased from the American Tissue Culture Collection (Manassas, VA, USA). Cells were cultured to 80% confluence in RPMI-1640 (Sigma-Aldrich, Ireland) supplemented with L-glutamine (2mM, All chemicals, unless otherwise stated, were purchased from Sigma-Aldrich, Ireland), HEPES (10mM; Invitrogen, NY, USA), sodium pyruvate (1mM), glucose (4.5g/L), sodium bicarbonate (1.5g/L) and foetal calf serum (10%; Invitrogen) in a humidified atmosphere at 37°C and 5% carbon dioxide. At 80% confluency, cells were subcultured by trypsinization using 0.25% trypsin-EDTA. Cells were then counted and resuspended in phosphate buffered saline (PBS) (Sigma-Aldrich, Ireland) at a concentration of 5 x 105/mL. Experiments were performed using three different cell passages.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from cells and tissues with the Qiagen RNeasy Midi Kit (Qiagen, UK), including a DNase digestion step, according to the manufacturer’s instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 3 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human U133 Plus 2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | The probe arrays were scanned at 560nm using a confocal laser-scanning microscope - Affymetrix Scanner 3000.
| | Sample_data_processing | Data was analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Annamarie,,Rogers
| | Sample_contact_email | annamarierogers@gmail.com
| | Sample_contact_phone | 0861911256
| | Sample_contact_laboratory | Molecular Biology
| | Sample_contact_department | Surgery
| | Sample_contact_institute | Trinity College Dublin
| | Sample_contact_address | AMNCH
| | Sample_contact_city | Dublin
| | Sample_contact_zip/postal_code | 24
| | Sample_contact_country | Ireland
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM567nnn/GSM567116/suppl/GSM567116.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM567nnn/GSM567116/suppl/GSM567116.CHP.gz
| | Sample_series_id | GSE22973
| | Sample_data_row_count | 54675
| | |
|
GSM567117 | GPL570 |
|
AsPC-1 control untreated cells, biological rep 3
|
Human Pancreatic Cancer Cell Line (ATCC)
|
cell passage: P12
cell line: AsPC-1
|
Human pancreatic cancer cells originally isolated from ascites. Untreated control cells.
|
| Sample_geo_accession | GSM567117
| | Sample_status | Public on Jan 01 2011
| | Sample_submission_date | Jul 16 2010
| | Sample_last_update_date | Jan 01 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | For gene expression studies, cells were serum starved overnight (2.5% foetal calf serum (FCS)) before treatment with a final concentration of PMA (1µM; reconsitituted in DMSO, 0.1%) for 4 hours. In parallel experiments, lysis buffer (350 μl; Qiagen, West Sussex, UK), containing β-mercaptoethanol (0.145 M) to avoid RNA degradation, was added to control and treated cells which were frozen at -80°C for isolation of total RNA
| | Sample_growth_protocol_ch1 | All cell culture reagents were purchased from Sarstedt (Wexford, Ireland). Human pancreatic cancer cell lines, AsPC-1 and BxPC-3, were purchased from the American Tissue Culture Collection (Manassas, VA, USA). Cells were cultured to 80% confluence in RPMI-1640 (Sigma-Aldrich, Ireland) supplemented with L-glutamine (2mM, All chemicals, unless otherwise stated, were purchased from Sigma-Aldrich, Ireland), HEPES (10mM; Invitrogen, NY, USA), sodium pyruvate (1mM), glucose (4.5g/L), sodium bicarbonate (1.5g/L) and foetal calf serum (10%; Invitrogen) in a humidified atmosphere at 37°C and 5% carbon dioxide. At 80% confluency, cells were subcultured by trypsinization using 0.25% trypsin-EDTA. Cells were then counted and resuspended in phosphate buffered saline (PBS) (Sigma-Aldrich, Ireland) at a concentration of 5 x 105/mL. Experiments were performed using three different cell passages.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from cells and tissues with the Qiagen RNeasy Midi Kit (Qiagen, UK), including a DNase digestion step, according to the manufacturer’s instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 3 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human U133 Plus 2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | The probe arrays were scanned at 560nm using a confocal laser-scanning microscope - Affymetrix Scanner 3000.
| | Sample_data_processing | Data was analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Annamarie,,Rogers
| | Sample_contact_email | annamarierogers@gmail.com
| | Sample_contact_phone | 0861911256
| | Sample_contact_laboratory | Molecular Biology
| | Sample_contact_department | Surgery
| | Sample_contact_institute | Trinity College Dublin
| | Sample_contact_address | AMNCH
| | Sample_contact_city | Dublin
| | Sample_contact_zip/postal_code | 24
| | Sample_contact_country | Ireland
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM567nnn/GSM567117/suppl/GSM567117.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM567nnn/GSM567117/suppl/GSM567117.CHP.gz
| | Sample_series_id | GSE22973
| | Sample_data_row_count | 54675
| | |
|
GSM567118 | GPL570 |
|
AsPC-1 + PMA (1µM; 4h), biological rep 1
|
Human Pancreatic Cancer Cell Line (ATCC)
|
cell passage: P8
cell line: AsPC-1
|
Human pancreatic cancer cells originally isolated from ascites. Cells were treated with PMA (1µM) for 4 hours.
|
| Sample_geo_accession | GSM567118
| | Sample_status | Public on Jan 01 2011
| | Sample_submission_date | Jul 16 2010
| | Sample_last_update_date | Jan 01 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | For gene expression studies, cells were serum starved overnight (2.5% foetal calf serum (FCS)) before treatment with a final concentration of PMA (1µM; reconsitituted in DMSO, 0.1%) for 4 hours. In parallel experiments, lysis buffer (350 μl; Qiagen, West Sussex, UK), containing β-mercaptoethanol (0.145 M) to avoid RNA degradation, was added to control and treated cells which were frozen at -80°C for isolation of total RNA
| | Sample_growth_protocol_ch1 | All cell culture reagents were purchased from Sarstedt (Wexford, Ireland). Human pancreatic cancer cell lines, AsPC-1 and BxPC-3, were purchased from the American Tissue Culture Collection (Manassas, VA, USA). Cells were cultured to 80% confluence in RPMI-1640 (Sigma-Aldrich, Ireland) supplemented with L-glutamine (2mM, All chemicals, unless otherwise stated, were purchased from Sigma-Aldrich, Ireland), HEPES (10mM; Invitrogen, NY, USA), sodium pyruvate (1mM), glucose (4.5g/L), sodium bicarbonate (1.5g/L) and foetal calf serum (10%; Invitrogen) in a humidified atmosphere at 37°C and 5% carbon dioxide. At 80% confluency, cells were subcultured by trypsinization using 0.25% trypsin-EDTA. Cells were then counted and resuspended in phosphate buffered saline (PBS) (Sigma-Aldrich, Ireland) at a concentration of 5 x 105/mL. Experiments were performed using three different cell passages.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from cells and tissues with the Qiagen RNeasy Midi Kit (Qiagen, UK), including a DNase digestion step, according to the manufacturer’s instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 3 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human U133 Plus 2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | The probe arrays were scanned at 560nm using a confocal laser-scanning microscope - Affymetrix Scanner 3000.
| | Sample_data_processing | Data was analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Annamarie,,Rogers
| | Sample_contact_email | annamarierogers@gmail.com
| | Sample_contact_phone | 0861911256
| | Sample_contact_laboratory | Molecular Biology
| | Sample_contact_department | Surgery
| | Sample_contact_institute | Trinity College Dublin
| | Sample_contact_address | AMNCH
| | Sample_contact_city | Dublin
| | Sample_contact_zip/postal_code | 24
| | Sample_contact_country | Ireland
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM567nnn/GSM567118/suppl/GSM567118.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM567nnn/GSM567118/suppl/GSM567118.CHP.gz
| | Sample_series_id | GSE22973
| | Sample_data_row_count | 54675
| | |
|
GSM567119 | GPL570 |
|
AsPC-1 + PMA (1µM; 4h), biological rep 2
|
Human Pancreatic Cancer Cell Line (ATCC)
|
cell passage: P10
cell line: AsPC-1
|
Human pancreatic cancer cells originally isolated from ascites. Cells were treated with PMA (1µM) for 4 hours.
|
| Sample_geo_accession | GSM567119
| | Sample_status | Public on Jan 01 2011
| | Sample_submission_date | Jul 16 2010
| | Sample_last_update_date | Jan 01 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | For gene expression studies, cells were serum starved overnight (2.5% foetal calf serum (FCS)) before treatment with a final concentration of PMA (1µM; reconsitituted in DMSO, 0.1%) for 4 hours. In parallel experiments, lysis buffer (350 μl; Qiagen, West Sussex, UK), containing β-mercaptoethanol (0.145 M) to avoid RNA degradation, was added to control and treated cells which were frozen at -80°C for isolation of total RNA
| | Sample_growth_protocol_ch1 | All cell culture reagents were purchased from Sarstedt (Wexford, Ireland). Human pancreatic cancer cell lines, AsPC-1 and BxPC-3, were purchased from the American Tissue Culture Collection (Manassas, VA, USA). Cells were cultured to 80% confluence in RPMI-1640 (Sigma-Aldrich, Ireland) supplemented with L-glutamine (2mM, All chemicals, unless otherwise stated, were purchased from Sigma-Aldrich, Ireland), HEPES (10mM; Invitrogen, NY, USA), sodium pyruvate (1mM), glucose (4.5g/L), sodium bicarbonate (1.5g/L) and foetal calf serum (10%; Invitrogen) in a humidified atmosphere at 37°C and 5% carbon dioxide. At 80% confluency, cells were subcultured by trypsinization using 0.25% trypsin-EDTA. Cells were then counted and resuspended in phosphate buffered saline (PBS) (Sigma-Aldrich, Ireland) at a concentration of 5 x 105/mL. Experiments were performed using three different cell passages.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from cells and tissues with the Qiagen RNeasy Midi Kit (Qiagen, UK), including a DNase digestion step, according to the manufacturer’s instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 3 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human U133 Plus 2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | The probe arrays were scanned at 560nm using a confocal laser-scanning microscope - Affymetrix Scanner 3000.
| | Sample_data_processing | Data was analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Annamarie,,Rogers
| | Sample_contact_email | annamarierogers@gmail.com
| | Sample_contact_phone | 0861911256
| | Sample_contact_laboratory | Molecular Biology
| | Sample_contact_department | Surgery
| | Sample_contact_institute | Trinity College Dublin
| | Sample_contact_address | AMNCH
| | Sample_contact_city | Dublin
| | Sample_contact_zip/postal_code | 24
| | Sample_contact_country | Ireland
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM567nnn/GSM567119/suppl/GSM567119.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM567nnn/GSM567119/suppl/GSM567119.CHP.gz
| | Sample_series_id | GSE22973
| | Sample_data_row_count | 54675
| | |
|
GSM567120 | GPL570 |
|
AsPC-1 + PMA (1µM; 4h), biological rep 3
|
Human Pancreatic Cancer Cell Line (ATCC)
|
cell passage: P12
cell line: AsPC-1
|
Human pancreatic cancer cells originally isolated from ascites. Cells were treated with PMA (1µM) for 4 hours.
|
| Sample_geo_accession | GSM567120
| | Sample_status | Public on Jan 01 2011
| | Sample_submission_date | Jul 16 2010
| | Sample_last_update_date | Jan 01 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | For gene expression studies, cells were serum starved overnight (2.5% foetal calf serum (FCS)) before treatment with a final concentration of PMA (1µM; reconsitituted in DMSO, 0.1%) for 4 hours. In parallel experiments, lysis buffer (350 μl; Qiagen, West Sussex, UK), containing β-mercaptoethanol (0.145 M) to avoid RNA degradation, was added to control and treated cells which were frozen at -80°C for isolation of total RNA
| | Sample_growth_protocol_ch1 | All cell culture reagents were purchased from Sarstedt (Wexford, Ireland). Human pancreatic cancer cell lines, AsPC-1 and BxPC-3, were purchased from the American Tissue Culture Collection (Manassas, VA, USA). Cells were cultured to 80% confluence in RPMI-1640 (Sigma-Aldrich, Ireland) supplemented with L-glutamine (2mM, All chemicals, unless otherwise stated, were purchased from Sigma-Aldrich, Ireland), HEPES (10mM; Invitrogen, NY, USA), sodium pyruvate (1mM), glucose (4.5g/L), sodium bicarbonate (1.5g/L) and foetal calf serum (10%; Invitrogen) in a humidified atmosphere at 37°C and 5% carbon dioxide. At 80% confluency, cells were subcultured by trypsinization using 0.25% trypsin-EDTA. Cells were then counted and resuspended in phosphate buffered saline (PBS) (Sigma-Aldrich, Ireland) at a concentration of 5 x 105/mL. Experiments were performed using three different cell passages.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from cells and tissues with the Qiagen RNeasy Midi Kit (Qiagen, UK), including a DNase digestion step, according to the manufacturer’s instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 3 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human U133 Plus 2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | The probe arrays were scanned at 560nm using a confocal laser-scanning microscope - Affymetrix Scanner 3000.
| | Sample_data_processing | Data was analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Annamarie,,Rogers
| | Sample_contact_email | annamarierogers@gmail.com
| | Sample_contact_phone | 0861911256
| | Sample_contact_laboratory | Molecular Biology
| | Sample_contact_department | Surgery
| | Sample_contact_institute | Trinity College Dublin
| | Sample_contact_address | AMNCH
| | Sample_contact_city | Dublin
| | Sample_contact_zip/postal_code | 24
| | Sample_contact_country | Ireland
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM567nnn/GSM567120/suppl/GSM567120.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM567nnn/GSM567120/suppl/GSM567120.CHP.gz
| | Sample_series_id | GSE22973
| | Sample_data_row_count | 54675
| | |
|
GSM567121 | GPL570 |
|
BxPC-3 control untreated cells, biological rep 1
|
Human Pancreatic Cancer Cell Line (ATCC)
|
cell passage: P7
cell line: BxPC-3
|
Human pancreatic cancer cells originally isolated from a primary tumour. Untreated control cells
|
| Sample_geo_accession | GSM567121
| | Sample_status | Public on Jan 01 2011
| | Sample_submission_date | Jul 16 2010
| | Sample_last_update_date | Jan 01 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | For gene expression studies, cells were serum starved overnight (2.5% foetal calf serum (FCS)) before treatment with a final concentration of PMA (1µM; reconsitituted in DMSO, 0.1%) for 4 hours. In parallel experiments, lysis buffer (350 μl; Qiagen, West Sussex, UK), containing β-mercaptoethanol (0.145 M) to avoid RNA degradation, was added to control and treated cells which were frozen at -80°C for isolation of total RNA
| | Sample_growth_protocol_ch1 | All cell culture reagents were purchased from Sarstedt (Wexford, Ireland). Human pancreatic cancer cell lines, AsPC-1 and BxPC-3, were purchased from the American Tissue Culture Collection (Manassas, VA, USA). Cells were cultured to 80% confluence in RPMI-1640 (Sigma-Aldrich, Ireland) supplemented with L-glutamine (2mM, All chemicals, unless otherwise stated, were purchased from Sigma-Aldrich, Ireland), HEPES (10mM; Invitrogen, NY, USA), sodium pyruvate (1mM), glucose (4.5g/L), sodium bicarbonate (1.5g/L) and foetal calf serum (10%; Invitrogen) in a humidified atmosphere at 37°C and 5% carbon dioxide. At 80% confluency, cells were subcultured by trypsinization using 0.25% trypsin-EDTA. Cells were then counted and resuspended in phosphate buffered saline (PBS) (Sigma-Aldrich, Ireland) at a concentration of 5 x 105/mL. Experiments were performed using three different cell passages.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from cells and tissues with the Qiagen RNeasy Midi Kit (Qiagen, UK), including a DNase digestion step, according to the manufacturer’s instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 3 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human U133 Plus 2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | The probe arrays were scanned at 560nm using a confocal laser-scanning microscope - Affymetrix Scanner 3000.
| | Sample_data_processing | Data was analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Annamarie,,Rogers
| | Sample_contact_email | annamarierogers@gmail.com
| | Sample_contact_phone | 0861911256
| | Sample_contact_laboratory | Molecular Biology
| | Sample_contact_department | Surgery
| | Sample_contact_institute | Trinity College Dublin
| | Sample_contact_address | AMNCH
| | Sample_contact_city | Dublin
| | Sample_contact_zip/postal_code | 24
| | Sample_contact_country | Ireland
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM567nnn/GSM567121/suppl/GSM567121.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM567nnn/GSM567121/suppl/GSM567121.CHP.gz
| | Sample_series_id | GSE22973
| | Sample_data_row_count | 54675
| | |
|
GSM567122 | GPL570 |
|
BxPC-3 control untreated cells, biological rep 2
|
Human Pancreatic Cancer Cell Line (ATCC)
|
cell passage: P9
cell line: BxPC-3
|
Human pancreatic cancer cells originally isolated from a primary tumour. Untreated control cells
|
| Sample_geo_accession | GSM567122
| | Sample_status | Public on Jan 01 2011
| | Sample_submission_date | Jul 16 2010
| | Sample_last_update_date | Jan 01 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | For gene expression studies, cells were serum starved overnight (2.5% foetal calf serum (FCS)) before treatment with a final concentration of PMA (1µM; reconsitituted in DMSO, 0.1%) for 4 hours. In parallel experiments, lysis buffer (350 μl; Qiagen, West Sussex, UK), containing β-mercaptoethanol (0.145 M) to avoid RNA degradation, was added to control and treated cells which were frozen at -80°C for isolation of total RNA
| | Sample_growth_protocol_ch1 | All cell culture reagents were purchased from Sarstedt (Wexford, Ireland). Human pancreatic cancer cell lines, AsPC-1 and BxPC-3, were purchased from the American Tissue Culture Collection (Manassas, VA, USA). Cells were cultured to 80% confluence in RPMI-1640 (Sigma-Aldrich, Ireland) supplemented with L-glutamine (2mM, All chemicals, unless otherwise stated, were purchased from Sigma-Aldrich, Ireland), HEPES (10mM; Invitrogen, NY, USA), sodium pyruvate (1mM), glucose (4.5g/L), sodium bicarbonate (1.5g/L) and foetal calf serum (10%; Invitrogen) in a humidified atmosphere at 37°C and 5% carbon dioxide. At 80% confluency, cells were subcultured by trypsinization using 0.25% trypsin-EDTA. Cells were then counted and resuspended in phosphate buffered saline (PBS) (Sigma-Aldrich, Ireland) at a concentration of 5 x 105/mL. Experiments were performed using three different cell passages.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from cells and tissues with the Qiagen RNeasy Midi Kit (Qiagen, UK), including a DNase digestion step, according to the manufacturer’s instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 3 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human U133 Plus 2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | The probe arrays were scanned at 560nm using a confocal laser-scanning microscope - Affymetrix Scanner 3000.
| | Sample_data_processing | Data was analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Annamarie,,Rogers
| | Sample_contact_email | annamarierogers@gmail.com
| | Sample_contact_phone | 0861911256
| | Sample_contact_laboratory | Molecular Biology
| | Sample_contact_department | Surgery
| | Sample_contact_institute | Trinity College Dublin
| | Sample_contact_address | AMNCH
| | Sample_contact_city | Dublin
| | Sample_contact_zip/postal_code | 24
| | Sample_contact_country | Ireland
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM567nnn/GSM567122/suppl/GSM567122.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM567nnn/GSM567122/suppl/GSM567122.CHP.gz
| | Sample_series_id | GSE22973
| | Sample_data_row_count | 54675
| | |
|
GSM567123 | GPL570 |
|
BxPC-3 control untreated cells, biological rep 3
|
Human Pancreatic Cancer Cell Line (ATCC)
|
cell passage: P11
cell line: BxPC-3
|
Human pancreatic cancer cells originally isolated from a primary tumour. Untreated control cells
|
| Sample_geo_accession | GSM567123
| | Sample_status | Public on Jan 01 2011
| | Sample_submission_date | Jul 16 2010
| | Sample_last_update_date | Jan 01 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | For gene expression studies, cells were serum starved overnight (2.5% foetal calf serum (FCS)) before treatment with a final concentration of PMA (1µM; reconsitituted in DMSO, 0.1%) for 4 hours. In parallel experiments, lysis buffer (350 μl; Qiagen, West Sussex, UK), containing β-mercaptoethanol (0.145 M) to avoid RNA degradation, was added to control and treated cells which were frozen at -80°C for isolation of total RNA
| | Sample_growth_protocol_ch1 | All cell culture reagents were purchased from Sarstedt (Wexford, Ireland). Human pancreatic cancer cell lines, AsPC-1 and BxPC-3, were purchased from the American Tissue Culture Collection (Manassas, VA, USA). Cells were cultured to 80% confluence in RPMI-1640 (Sigma-Aldrich, Ireland) supplemented with L-glutamine (2mM, All chemicals, unless otherwise stated, were purchased from Sigma-Aldrich, Ireland), HEPES (10mM; Invitrogen, NY, USA), sodium pyruvate (1mM), glucose (4.5g/L), sodium bicarbonate (1.5g/L) and foetal calf serum (10%; Invitrogen) in a humidified atmosphere at 37°C and 5% carbon dioxide. At 80% confluency, cells were subcultured by trypsinization using 0.25% trypsin-EDTA. Cells were then counted and resuspended in phosphate buffered saline (PBS) (Sigma-Aldrich, Ireland) at a concentration of 5 x 105/mL. Experiments were performed using three different cell passages.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from cells and tissues with the Qiagen RNeasy Midi Kit (Qiagen, UK), including a DNase digestion step, according to the manufacturer’s instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 3 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human U133 Plus 2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | The probe arrays were scanned at 560nm using a confocal laser-scanning microscope - Affymetrix Scanner 3000.
| | Sample_data_processing | Data was analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Annamarie,,Rogers
| | Sample_contact_email | annamarierogers@gmail.com
| | Sample_contact_phone | 0861911256
| | Sample_contact_laboratory | Molecular Biology
| | Sample_contact_department | Surgery
| | Sample_contact_institute | Trinity College Dublin
| | Sample_contact_address | AMNCH
| | Sample_contact_city | Dublin
| | Sample_contact_zip/postal_code | 24
| | Sample_contact_country | Ireland
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM567nnn/GSM567123/suppl/GSM567123.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM567nnn/GSM567123/suppl/GSM567123.CHP.gz
| | Sample_series_id | GSE22973
| | Sample_data_row_count | 54675
| | |
|
GSM567124 | GPL570 |
|
BxPC-3 + PMA (1µM; 4h), biological rep 1
|
Human Pancreatic Cancer Cell Line (ATCC)
|
cell passage: P7
cell line: BxPC-3
|
Human pancreatic cancer cells originally isolated from a primary tumour. Cells were treated with PMA (1µM) for 4 hours.
|
| Sample_geo_accession | GSM567124
| | Sample_status | Public on Jan 01 2011
| | Sample_submission_date | Jul 16 2010
| | Sample_last_update_date | Jan 01 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | For gene expression studies, cells were serum starved overnight (2.5% foetal calf serum (FCS)) before treatment with a final concentration of PMA (1µM; reconsitituted in DMSO, 0.1%) for 4 hours. In parallel experiments, lysis buffer (350 μl; Qiagen, West Sussex, UK), containing β-mercaptoethanol (0.145 M) to avoid RNA degradation, was added to control and treated cells which were frozen at -80°C for isolation of total RNA
| | Sample_growth_protocol_ch1 | All cell culture reagents were purchased from Sarstedt (Wexford, Ireland). Human pancreatic cancer cell lines, AsPC-1 and BxPC-3, were purchased from the American Tissue Culture Collection (Manassas, VA, USA). Cells were cultured to 80% confluence in RPMI-1640 (Sigma-Aldrich, Ireland) supplemented with L-glutamine (2mM, All chemicals, unless otherwise stated, were purchased from Sigma-Aldrich, Ireland), HEPES (10mM; Invitrogen, NY, USA), sodium pyruvate (1mM), glucose (4.5g/L), sodium bicarbonate (1.5g/L) and foetal calf serum (10%; Invitrogen) in a humidified atmosphere at 37°C and 5% carbon dioxide. At 80% confluency, cells were subcultured by trypsinization using 0.25% trypsin-EDTA. Cells were then counted and resuspended in phosphate buffered saline (PBS) (Sigma-Aldrich, Ireland) at a concentration of 5 x 105/mL. Experiments were performed using three different cell passages.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from cells and tissues with the Qiagen RNeasy Midi Kit (Qiagen, UK), including a DNase digestion step, according to the manufacturer’s instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 3 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human U133 Plus 2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | The probe arrays were scanned at 560nm using a confocal laser-scanning microscope - Affymetrix Scanner 3000.
| | Sample_data_processing | Data was analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Annamarie,,Rogers
| | Sample_contact_email | annamarierogers@gmail.com
| | Sample_contact_phone | 0861911256
| | Sample_contact_laboratory | Molecular Biology
| | Sample_contact_department | Surgery
| | Sample_contact_institute | Trinity College Dublin
| | Sample_contact_address | AMNCH
| | Sample_contact_city | Dublin
| | Sample_contact_zip/postal_code | 24
| | Sample_contact_country | Ireland
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM567nnn/GSM567124/suppl/GSM567124.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM567nnn/GSM567124/suppl/GSM567124.CHP.gz
| | Sample_series_id | GSE22973
| | Sample_data_row_count | 54675
| | |
|
GSM567125 | GPL570 |
|
BxPC-3 + PMA (1µM; 4h), biological rep 2
|
Human Pancreatic Cancer Cell Line (ATCC)
|
cell passage: P9
cell line: BxPC-3
|
Human pancreatic cancer cells originally isolated from a primary tumour. Cells were treated with PMA (1µM) for 4 hours.
|
| Sample_geo_accession | GSM567125
| | Sample_status | Public on Jan 01 2011
| | Sample_submission_date | Jul 16 2010
| | Sample_last_update_date | Jan 01 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | For gene expression studies, cells were serum starved overnight (2.5% foetal calf serum (FCS)) before treatment with a final concentration of PMA (1µM; reconsitituted in DMSO, 0.1%) for 4 hours. In parallel experiments, lysis buffer (350 μl; Qiagen, West Sussex, UK), containing β-mercaptoethanol (0.145 M) to avoid RNA degradation, was added to control and treated cells which were frozen at -80°C for isolation of total RNA
| | Sample_growth_protocol_ch1 | All cell culture reagents were purchased from Sarstedt (Wexford, Ireland). Human pancreatic cancer cell lines, AsPC-1 and BxPC-3, were purchased from the American Tissue Culture Collection (Manassas, VA, USA). Cells were cultured to 80% confluence in RPMI-1640 (Sigma-Aldrich, Ireland) supplemented with L-glutamine (2mM, All chemicals, unless otherwise stated, were purchased from Sigma-Aldrich, Ireland), HEPES (10mM; Invitrogen, NY, USA), sodium pyruvate (1mM), glucose (4.5g/L), sodium bicarbonate (1.5g/L) and foetal calf serum (10%; Invitrogen) in a humidified atmosphere at 37°C and 5% carbon dioxide. At 80% confluency, cells were subcultured by trypsinization using 0.25% trypsin-EDTA. Cells were then counted and resuspended in phosphate buffered saline (PBS) (Sigma-Aldrich, Ireland) at a concentration of 5 x 105/mL. Experiments were performed using three different cell passages.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from cells and tissues with the Qiagen RNeasy Midi Kit (Qiagen, UK), including a DNase digestion step, according to the manufacturer’s instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 3 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human U133 Plus 2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | The probe arrays were scanned at 560nm using a confocal laser-scanning microscope - Affymetrix Scanner 3000.
| | Sample_data_processing | Data was analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Annamarie,,Rogers
| | Sample_contact_email | annamarierogers@gmail.com
| | Sample_contact_phone | 0861911256
| | Sample_contact_laboratory | Molecular Biology
| | Sample_contact_department | Surgery
| | Sample_contact_institute | Trinity College Dublin
| | Sample_contact_address | AMNCH
| | Sample_contact_city | Dublin
| | Sample_contact_zip/postal_code | 24
| | Sample_contact_country | Ireland
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM567nnn/GSM567125/suppl/GSM567125.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM567nnn/GSM567125/suppl/GSM567125.CHP.gz
| | Sample_series_id | GSE22973
| | Sample_data_row_count | 54675
| | |
|
GSM567126 | GPL570 |
|
BxPC-3 + PMA (1µM; 4h), biological rep 3
|
Human Pancreatic Cancer Cell Line (ATCC)
|
cell passage: P11
cell line: BxPC-3
|
Human pancreatic cancer cells originally isolated from a primary tumour. Cells were treated with PMA (1µM) for 4 hours.
|
| Sample_geo_accession | GSM567126
| | Sample_status | Public on Jan 01 2011
| | Sample_submission_date | Jul 16 2010
| | Sample_last_update_date | Jan 01 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | For gene expression studies, cells were serum starved overnight (2.5% foetal calf serum (FCS)) before treatment with a final concentration of PMA (1µM; reconsitituted in DMSO, 0.1%) for 4 hours. In parallel experiments, lysis buffer (350 μl; Qiagen, West Sussex, UK), containing β-mercaptoethanol (0.145 M) to avoid RNA degradation, was added to control and treated cells which were frozen at -80°C for isolation of total RNA
| | Sample_growth_protocol_ch1 | All cell culture reagents were purchased from Sarstedt (Wexford, Ireland). Human pancreatic cancer cell lines, AsPC-1 and BxPC-3, were purchased from the American Tissue Culture Collection (Manassas, VA, USA). Cells were cultured to 80% confluence in RPMI-1640 (Sigma-Aldrich, Ireland) supplemented with L-glutamine (2mM, All chemicals, unless otherwise stated, were purchased from Sigma-Aldrich, Ireland), HEPES (10mM; Invitrogen, NY, USA), sodium pyruvate (1mM), glucose (4.5g/L), sodium bicarbonate (1.5g/L) and foetal calf serum (10%; Invitrogen) in a humidified atmosphere at 37°C and 5% carbon dioxide. At 80% confluency, cells were subcultured by trypsinization using 0.25% trypsin-EDTA. Cells were then counted and resuspended in phosphate buffered saline (PBS) (Sigma-Aldrich, Ireland) at a concentration of 5 x 105/mL. Experiments were performed using three different cell passages.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from cells and tissues with the Qiagen RNeasy Midi Kit (Qiagen, UK), including a DNase digestion step, according to the manufacturer’s instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 3 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human U133 Plus 2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | The probe arrays were scanned at 560nm using a confocal laser-scanning microscope - Affymetrix Scanner 3000.
| | Sample_data_processing | Data was analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Annamarie,,Rogers
| | Sample_contact_email | annamarierogers@gmail.com
| | Sample_contact_phone | 0861911256
| | Sample_contact_laboratory | Molecular Biology
| | Sample_contact_department | Surgery
| | Sample_contact_institute | Trinity College Dublin
| | Sample_contact_address | AMNCH
| | Sample_contact_city | Dublin
| | Sample_contact_zip/postal_code | 24
| | Sample_contact_country | Ireland
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM567nnn/GSM567126/suppl/GSM567126.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM567nnn/GSM567126/suppl/GSM567126.CHP.gz
| | Sample_series_id | GSE22973
| | Sample_data_row_count | 54675
| | |
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