Result

Search results for the GEO ID: GSE23161

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GSM ID

GPL ID

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Title

Source name

Description

Characteristics

GSM570217

GPL1261

KsJ_HF rep 1

KsJ High fat fed replicate 1

tissue: Pancreatic beta cells strain: C57/Bl6J genotype/variation: KsJ

Gene expression in beta cells of high fat fed KsJ replicate 1

Sample_geo_accession	GSM570217
Sample_status	Public on Jun 30 2011
Sample_submission_date	Jul 26 2010
Sample_last_update_date	Jun 30 2011
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	KsJ wild type and MIP-HSD1 heterozygous mice were high fat fed for 12 weeks. Mice were given 58% of saturated fat as energy with sucrose (D12331, Research Diets, New Brunswick, NJ) for 12 weeks from 4 weeks of age.
Sample_growth_protocol_ch1	Mice (all male) were housed in standard conditions on a 12 h light/dark cycle and fed standard rodent chow (Special Diet Services, Edinburgh, UK).
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Pancreata perfused with collagenase and islets isolated by hand picking. Isolated islets (around 500) coming from at least 3 mice (around 200/mice) have been directly lysed in Trizol to extract total RNA.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Affymetrix IVT Express
Sample_hyb_protocol	Hybridised for overnight at 45C on Mouse MOE 430_2 GeneChips. GeneChips were processed with standard Affymetric Protocols.
Sample_scan_protocol	GeneChips scanned with HP GeneArray Scanner
Sample_data_processing	CEL files imported into Affy in Bioconductor. Normalisation with RMA (with background subtraction). Normalised gene expression values (RMA with background subtraction) were converted to linear scale from log2 scale. R v2.8.0, Biobase_2.2.2, and affy_1.20.2 were used.
Sample_platform_id	GPL1261
Sample_contact_name	Donald,,Dunbar
Sample_contact_email	donald.dunbar@ed.ac.uk
Sample_contact_phone	+44 131 242 6700
Sample_contact_laboratory	Bioinformatics
Sample_contact_department	Centre for Cardiovascular Science
Sample_contact_institute	University of Edinburgh
Sample_contact_address	47 Little France Crescent
Sample_contact_city	Edinburgh
Sample_contact_zip/postal_code	EH16 4TJ
Sample_contact_country	United Kingdom
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM570nnn/GSM570217/suppl/GSM570217.CEL.gz
Sample_series_id	GSE23161
Sample_data_row_count	45101

GSM570218

GPL1261

KsJ_HF rep 2

KsJ High fat fed replicate 2

tissue: Pancreatic beta cells strain: C57/Bl6J genotype/variation: KsJ

Gene expression in beta cells of high fat fed KsJ replicate 2

Sample_geo_accession	GSM570218
Sample_status	Public on Jun 30 2011
Sample_submission_date	Jul 26 2010
Sample_last_update_date	Jun 30 2011
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	KsJ wild type and MIP-HSD1 heterozygous mice were high fat fed for 12 weeks. Mice were given 58% of saturated fat as energy with sucrose (D12331, Research Diets, New Brunswick, NJ) for 12 weeks from 4 weeks of age.
Sample_growth_protocol_ch1	Mice (all male) were housed in standard conditions on a 12 h light/dark cycle and fed standard rodent chow (Special Diet Services, Edinburgh, UK).
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Pancreata perfused with collagenase and islets isolated by hand picking. Isolated islets (around 500) coming from at least 3 mice (around 200/mice) have been directly lysed in Trizol to extract total RNA.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Affymetrix IVT Express
Sample_hyb_protocol	Hybridised for overnight at 45C on Mouse MOE 430_2 GeneChips. GeneChips were processed with standard Affymetric Protocols.
Sample_scan_protocol	GeneChips scanned with HP GeneArray Scanner
Sample_data_processing	CEL files imported into Affy in Bioconductor. Normalisation with RMA (with background subtraction). Normalised gene expression values (RMA with background subtraction) were converted to linear scale from log2 scale. R v2.8.0, Biobase_2.2.2, and affy_1.20.2 were used.
Sample_platform_id	GPL1261
Sample_contact_name	Donald,,Dunbar
Sample_contact_email	donald.dunbar@ed.ac.uk
Sample_contact_phone	+44 131 242 6700
Sample_contact_laboratory	Bioinformatics
Sample_contact_department	Centre for Cardiovascular Science
Sample_contact_institute	University of Edinburgh
Sample_contact_address	47 Little France Crescent
Sample_contact_city	Edinburgh
Sample_contact_zip/postal_code	EH16 4TJ
Sample_contact_country	United Kingdom
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM570nnn/GSM570218/suppl/GSM570218.CEL.gz
Sample_series_id	GSE23161
Sample_data_row_count	45101

GSM570219

GPL1261

KsJ_HF rep 3

KsJ High fat fed replicate 3

tissue: Pancreatic beta cells strain: C57/Bl6J genotype/variation: KsJ

Gene expression in beta cells of high fat fed KsJ replicate 3

Sample_geo_accession	GSM570219
Sample_status	Public on Jun 30 2011
Sample_submission_date	Jul 26 2010
Sample_last_update_date	Jun 30 2011
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	KsJ wild type and MIP-HSD1 heterozygous mice were high fat fed for 12 weeks. Mice were given 58% of saturated fat as energy with sucrose (D12331, Research Diets, New Brunswick, NJ) for 12 weeks from 4 weeks of age.
Sample_growth_protocol_ch1	Mice (all male) were housed in standard conditions on a 12 h light/dark cycle and fed standard rodent chow (Special Diet Services, Edinburgh, UK).
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Pancreata perfused with collagenase and islets isolated by hand picking. Isolated islets (around 500) coming from at least 3 mice (around 200/mice) have been directly lysed in Trizol to extract total RNA.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Affymetrix IVT Express
Sample_hyb_protocol	Hybridised for overnight at 45C on Mouse MOE 430_2 GeneChips. GeneChips were processed with standard Affymetric Protocols.
Sample_scan_protocol	GeneChips scanned with HP GeneArray Scanner
Sample_data_processing	CEL files imported into Affy in Bioconductor. Normalisation with RMA (with background subtraction). Normalised gene expression values (RMA with background subtraction) were converted to linear scale from log2 scale. R v2.8.0, Biobase_2.2.2, and affy_1.20.2 were used.
Sample_platform_id	GPL1261
Sample_contact_name	Donald,,Dunbar
Sample_contact_email	donald.dunbar@ed.ac.uk
Sample_contact_phone	+44 131 242 6700
Sample_contact_laboratory	Bioinformatics
Sample_contact_department	Centre for Cardiovascular Science
Sample_contact_institute	University of Edinburgh
Sample_contact_address	47 Little France Crescent
Sample_contact_city	Edinburgh
Sample_contact_zip/postal_code	EH16 4TJ
Sample_contact_country	United Kingdom
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM570nnn/GSM570219/suppl/GSM570219.CEL.gz
Sample_series_id	GSE23161
Sample_data_row_count	45101

GSM570220

GPL1261

KsJ_HF rep 4

KsJ High fat fed replicate 4

tissue: Pancreatic beta cells strain: C57/Bl6J genotype/variation: KsJ

Gene expression in beta cells of high fat fed KsJ replicate 4

Sample_geo_accession	GSM570220
Sample_status	Public on Jun 30 2011
Sample_submission_date	Jul 26 2010
Sample_last_update_date	Jun 30 2011
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	KsJ wild type and MIP-HSD1 heterozygous mice were high fat fed for 12 weeks. Mice were given 58% of saturated fat as energy with sucrose (D12331, Research Diets, New Brunswick, NJ) for 12 weeks from 4 weeks of age.
Sample_growth_protocol_ch1	Mice (all male) were housed in standard conditions on a 12 h light/dark cycle and fed standard rodent chow (Special Diet Services, Edinburgh, UK).
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Pancreata perfused with collagenase and islets isolated by hand picking. Isolated islets (around 500) coming from at least 3 mice (around 200/mice) have been directly lysed in Trizol to extract total RNA.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Affymetrix IVT Express
Sample_hyb_protocol	Hybridised for overnight at 45C on Mouse MOE 430_2 GeneChips. GeneChips were processed with standard Affymetric Protocols.
Sample_scan_protocol	GeneChips scanned with HP GeneArray Scanner
Sample_data_processing	CEL files imported into Affy in Bioconductor. Normalisation with RMA (with background subtraction). Normalised gene expression values (RMA with background subtraction) were converted to linear scale from log2 scale. R v2.8.0, Biobase_2.2.2, and affy_1.20.2 were used.
Sample_platform_id	GPL1261
Sample_contact_name	Donald,,Dunbar
Sample_contact_email	donald.dunbar@ed.ac.uk
Sample_contact_phone	+44 131 242 6700
Sample_contact_laboratory	Bioinformatics
Sample_contact_department	Centre for Cardiovascular Science
Sample_contact_institute	University of Edinburgh
Sample_contact_address	47 Little France Crescent
Sample_contact_city	Edinburgh
Sample_contact_zip/postal_code	EH16 4TJ
Sample_contact_country	United Kingdom
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM570nnn/GSM570220/suppl/GSM570220.CEL.gz
Sample_series_id	GSE23161
Sample_data_row_count	45101

GSM570221

GPL1261

MIP_HF rep 1

MIP High fat fed replicate 1

tissue: Pancreatic beta cells strain: C57/Bl6J genotype/variation: MIP-HSD1

Gene expression in beta cells of high fat fed MIP replicate 1

Sample_geo_accession	GSM570221
Sample_status	Public on Jun 30 2011
Sample_submission_date	Jul 26 2010
Sample_last_update_date	Jun 30 2011
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	KsJ wild type and MIP-HSD1 heterozygous mice were high fat fed for 12 weeks. Mice were given 58% of saturated fat as energy with sucrose (D12331, Research Diets, New Brunswick, NJ) for 12 weeks from 4 weeks of age.
Sample_growth_protocol_ch1	Mice (all male) were housed in standard conditions on a 12 h light/dark cycle and fed standard rodent chow (Special Diet Services, Edinburgh, UK).
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Pancreata perfused with collagenase and islets isolated by hand picking. Isolated islets (around 500) coming from at least 3 mice (around 200/mice) have been directly lysed in Trizol to extract total RNA.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Affymetrix IVT Express
Sample_hyb_protocol	Hybridised for overnight at 45C on Mouse MOE 430_2 GeneChips. GeneChips were processed with standard Affymetric Protocols.
Sample_scan_protocol	GeneChips scanned with HP GeneArray Scanner
Sample_data_processing	CEL files imported into Affy in Bioconductor. Normalisation with RMA (with background subtraction). Normalised gene expression values (RMA with background subtraction) were converted to linear scale from log2 scale. R v2.8.0, Biobase_2.2.2, and affy_1.20.2 were used.
Sample_platform_id	GPL1261
Sample_contact_name	Donald,,Dunbar
Sample_contact_email	donald.dunbar@ed.ac.uk
Sample_contact_phone	+44 131 242 6700
Sample_contact_laboratory	Bioinformatics
Sample_contact_department	Centre for Cardiovascular Science
Sample_contact_institute	University of Edinburgh
Sample_contact_address	47 Little France Crescent
Sample_contact_city	Edinburgh
Sample_contact_zip/postal_code	EH16 4TJ
Sample_contact_country	United Kingdom
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM570nnn/GSM570221/suppl/GSM570221.CEL.gz
Sample_series_id	GSE23161
Sample_data_row_count	45101

GSM570222

GPL1261

MIP_HF rep 2

MIP High fat fed replicate 2

tissue: Pancreatic beta cells strain: C57/Bl6J genotype/variation: MIP-HSD1

Gene expression in beta cells of high fat fed MIP replicate 2

Sample_geo_accession	GSM570222
Sample_status	Public on Jun 30 2011
Sample_submission_date	Jul 26 2010
Sample_last_update_date	Jun 30 2011
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	KsJ wild type and MIP-HSD1 heterozygous mice were high fat fed for 12 weeks. Mice were given 58% of saturated fat as energy with sucrose (D12331, Research Diets, New Brunswick, NJ) for 12 weeks from 4 weeks of age.
Sample_growth_protocol_ch1	Mice (all male) were housed in standard conditions on a 12 h light/dark cycle and fed standard rodent chow (Special Diet Services, Edinburgh, UK).
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Pancreata perfused with collagenase and islets isolated by hand picking. Isolated islets (around 500) coming from at least 3 mice (around 200/mice) have been directly lysed in Trizol to extract total RNA.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Affymetrix IVT Express
Sample_hyb_protocol	Hybridised for overnight at 45C on Mouse MOE 430_2 GeneChips. GeneChips were processed with standard Affymetric Protocols.
Sample_scan_protocol	GeneChips scanned with HP GeneArray Scanner
Sample_data_processing	CEL files imported into Affy in Bioconductor. Normalisation with RMA (with background subtraction). Normalised gene expression values (RMA with background subtraction) were converted to linear scale from log2 scale. R v2.8.0, Biobase_2.2.2, and affy_1.20.2 were used.
Sample_platform_id	GPL1261
Sample_contact_name	Donald,,Dunbar
Sample_contact_email	donald.dunbar@ed.ac.uk
Sample_contact_phone	+44 131 242 6700
Sample_contact_laboratory	Bioinformatics
Sample_contact_department	Centre for Cardiovascular Science
Sample_contact_institute	University of Edinburgh
Sample_contact_address	47 Little France Crescent
Sample_contact_city	Edinburgh
Sample_contact_zip/postal_code	EH16 4TJ
Sample_contact_country	United Kingdom
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM570nnn/GSM570222/suppl/GSM570222.CEL.gz
Sample_series_id	GSE23161
Sample_data_row_count	45101

GSM570223

GPL1261

MIP_HF rep 3

MIP High fat fed replicate 3

tissue: Pancreatic beta cells strain: C57/Bl6J genotype/variation: MIP-HSD1

Gene expression in beta cells of high fat fed MIP replicate 3

Sample_geo_accession	GSM570223
Sample_status	Public on Jun 30 2011
Sample_submission_date	Jul 26 2010
Sample_last_update_date	Jun 30 2011
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	KsJ wild type and MIP-HSD1 heterozygous mice were high fat fed for 12 weeks. Mice were given 58% of saturated fat as energy with sucrose (D12331, Research Diets, New Brunswick, NJ) for 12 weeks from 4 weeks of age.
Sample_growth_protocol_ch1	Mice (all male) were housed in standard conditions on a 12 h light/dark cycle and fed standard rodent chow (Special Diet Services, Edinburgh, UK).
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Pancreata perfused with collagenase and islets isolated by hand picking. Isolated islets (around 500) coming from at least 3 mice (around 200/mice) have been directly lysed in Trizol to extract total RNA.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Affymetrix IVT Express
Sample_hyb_protocol	Hybridised for overnight at 45C on Mouse MOE 430_2 GeneChips. GeneChips were processed with standard Affymetric Protocols.
Sample_scan_protocol	GeneChips scanned with HP GeneArray Scanner
Sample_data_processing	CEL files imported into Affy in Bioconductor. Normalisation with RMA (with background subtraction). Normalised gene expression values (RMA with background subtraction) were converted to linear scale from log2 scale. R v2.8.0, Biobase_2.2.2, and affy_1.20.2 were used.
Sample_platform_id	GPL1261
Sample_contact_name	Donald,,Dunbar
Sample_contact_email	donald.dunbar@ed.ac.uk
Sample_contact_phone	+44 131 242 6700
Sample_contact_laboratory	Bioinformatics
Sample_contact_department	Centre for Cardiovascular Science
Sample_contact_institute	University of Edinburgh
Sample_contact_address	47 Little France Crescent
Sample_contact_city	Edinburgh
Sample_contact_zip/postal_code	EH16 4TJ
Sample_contact_country	United Kingdom
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM570nnn/GSM570223/suppl/GSM570223.CEL.gz
Sample_series_id	GSE23161
Sample_data_row_count	45101

GSM570224

GPL1261

MIP_HF rep 4

MIP High fat fed replicate 4

tissue: Pancreatic beta cells strain: C57/Bl6J genotype/variation: MIP-HSD1

Gene expression in beta cells of high fat fed MIP replicate 4

Sample_geo_accession	GSM570224
Sample_status	Public on Jun 30 2011
Sample_submission_date	Jul 26 2010
Sample_last_update_date	Jun 30 2011
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	KsJ wild type and MIP-HSD1 heterozygous mice were high fat fed for 12 weeks. Mice were given 58% of saturated fat as energy with sucrose (D12331, Research Diets, New Brunswick, NJ) for 12 weeks from 4 weeks of age.
Sample_growth_protocol_ch1	Mice (all male) were housed in standard conditions on a 12 h light/dark cycle and fed standard rodent chow (Special Diet Services, Edinburgh, UK).
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Pancreata perfused with collagenase and islets isolated by hand picking. Isolated islets (around 500) coming from at least 3 mice (around 200/mice) have been directly lysed in Trizol to extract total RNA.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Affymetrix IVT Express
Sample_hyb_protocol	Hybridised for overnight at 45C on Mouse MOE 430_2 GeneChips. GeneChips were processed with standard Affymetric Protocols.
Sample_scan_protocol	GeneChips scanned with HP GeneArray Scanner
Sample_data_processing	CEL files imported into Affy in Bioconductor. Normalisation with RMA (with background subtraction). Normalised gene expression values (RMA with background subtraction) were converted to linear scale from log2 scale. R v2.8.0, Biobase_2.2.2, and affy_1.20.2 were used.
Sample_platform_id	GPL1261
Sample_contact_name	Donald,,Dunbar
Sample_contact_email	donald.dunbar@ed.ac.uk
Sample_contact_phone	+44 131 242 6700
Sample_contact_laboratory	Bioinformatics
Sample_contact_department	Centre for Cardiovascular Science
Sample_contact_institute	University of Edinburgh
Sample_contact_address	47 Little France Crescent
Sample_contact_city	Edinburgh
Sample_contact_zip/postal_code	EH16 4TJ
Sample_contact_country	United Kingdom
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM570nnn/GSM570224/suppl/GSM570224.CEL.gz
Sample_series_id	GSE23161
Sample_data_row_count	45101

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