Search results for the GEO ID: GSE23206  |
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GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM570906 | GPL570 |
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H322c DMSO
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H322c after 4 days cultured with 0.1% DMSO
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cell line: H322c
treatment: DMSO
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Gene expression data from H322c cells not treated with EGFR TKI, gefitinib
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| Sample_geo_accession | GSM570906
| | Sample_status | Public on Jan 27 2011
| | Sample_submission_date | Jul 27 2010
| | Sample_last_update_date | Jan 27 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | 10μL of 1mM gefitinib or 10μL of DMSO was added to 10mL of RPMI 10% FBS media to give a final concentration of 1μM gefitinib or 0.1% DMSO
| | Sample_growth_protocol_ch1 | H322c cells were plated 24 hrs prior to DMSO or gefitinib addition to allow cells to adhere
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted from cells using the RNAeasy Mini Kit from Qiagen according to manufacteur's protocol
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 ug total RNA (Expression Analysis Technical Manual, 2002, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 11.5 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix human U133 plus 2.0 array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix Genechip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Kathryn,,Ware
| | Sample_contact_email | kathryn.ware@ucdenver.edu
| | Sample_contact_phone | 303-724-4632
| | Sample_contact_laboratory | Lynn Heasley
| | Sample_contact_department | Craniofacial Biology
| | Sample_contact_institute | University of Colorado Denver
| | Sample_contact_address | 12801 E 17th Ave, L18-11402A
| | Sample_contact_city | Aurora
| | Sample_contact_state | CO
| | Sample_contact_zip/postal_code | 80045
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM570nnn/GSM570906/suppl/GSM570906_heasley_5_H322C_dmso_HG-U133_Plus_2.CEL.gz
| | Sample_series_id | GSE23206
| | Sample_data_row_count | 54675
| | |
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GSM570907 | GPL570 |
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H322c 1μM Gefitinib
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H322c after 4 days cultured with 1μM gefitinib
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cell line: H322c
treatment: Gefitinib
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Gene expression data from H322c cells treated with EGFR TKI, gefitinib
|
| Sample_geo_accession | GSM570907
| | Sample_status | Public on Jan 27 2011
| | Sample_submission_date | Jul 27 2010
| | Sample_last_update_date | Jan 27 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | 10μL of 1mM gefitinib or 10μL of DMSO was added to 10mL of RPMI 10% FBS media to give a final concentration of 1μM gefitinib or 0.1% DMSO
| | Sample_growth_protocol_ch1 | H322c cells were plated 24 hrs prior to DMSO or gefitinib addition to allow cells to adhere
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted from cells using the RNAeasy Mini Kit from Qiagen according to manufacteur's protocol
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 ug total RNA (Expression Analysis Technical Manual, 2002, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 11.5 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix human U133 plus 2.0 array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix Genechip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Kathryn,,Ware
| | Sample_contact_email | kathryn.ware@ucdenver.edu
| | Sample_contact_phone | 303-724-4632
| | Sample_contact_laboratory | Lynn Heasley
| | Sample_contact_department | Craniofacial Biology
| | Sample_contact_institute | University of Colorado Denver
| | Sample_contact_address | 12801 E 17th Ave, L18-11402A
| | Sample_contact_city | Aurora
| | Sample_contact_state | CO
| | Sample_contact_zip/postal_code | 80045
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM570nnn/GSM570907/suppl/GSM570907_heasley_7_H322C_GEF_HG-U133_Plus_2.CEL.gz
| | Sample_series_id | GSE23206
| | Sample_data_row_count | 54675
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