Search results for the GEO ID: GSE23305  |
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GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM571897 | GPL1261 |
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Wild type fetal liver macrophage
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fetal liver macrophage
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strain: C57BL/6J
cell type: Fetal liver cells
genotype/variation: wild type
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Fetal liver cells from E15.5 c-Maf+/+ and c-Maf-/- embryos were incubated with APC-conjugated anti-Mac-1 antibody (eBioscience) and PE-conjugated anti-F4/80 antibody (Serotec). Mac-1+F4/80+ cells were sorted by a FACSAria cell sorter (Becton Dickinson) as fetal liver macrophages.
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| Sample_geo_accession | GSM571897
| | Sample_status | Public on Jul 06 2011
| | Sample_submission_date | Jul 28 2010
| | Sample_last_update_date | Jul 06 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | None.
| | Sample_growth_protocol_ch1 | None.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from fetal liver macrophages using a QIAGEN RNeasy Mini Kit according to the manufacturer’s instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | The microarray procedure was performed as described in the Affymetrix GeneChip Expression Analysis Manual (Affymetrix).
| | Sample_hyb_protocol | Biotin-labeled cRNA was synthesized from 4 to 6 μg RNA and hybridized to a GeneChip Mouse Genome 430 2.0 Array (Affymetrix).
| | Sample_scan_protocol | After washing, arrays were stained with streptavidin-phycoethrin and analysed on a scanner.
| | Sample_data_processing | The data processing has been performed according to the manufacture's protocol (Affymetrix). Affymetrix Algorithm: ExpressionStat 5.0
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Manabu,,Kusakabe
| | Sample_contact_phone | 81-29-853-6963
| | Sample_contact_fax | 81-29-853-6965
| | Sample_contact_laboratory | Takahashi Laboratory
| | Sample_contact_department | Department of Anatomy and Embryology
| | Sample_contact_institute | University of Tsukuba
| | Sample_contact_address | 1-1-1, Tennodai,
| | Sample_contact_city | Tsukuba
| | Sample_contact_state | IBARAKI
| | Sample_contact_zip/postal_code | 305-8575
| | Sample_contact_country | Japan
| | Sample_contact_web_link | http://www.md.tsukuba.ac.jp/basic-med/anatomy/embryology/index_e.html
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM571nnn/GSM571897/suppl/GSM571897.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM571nnn/GSM571897/suppl/GSM571897.CHP.gz
| | Sample_series_id | GSE23305
| | Sample_data_row_count | 45101
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GSM571898 | GPL1261 |
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c-Maf KO fetal liver macrophage
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fetal liver macrophage
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strain: C57BL/6J
cell type: Fetal liver cells
genotype/variation: c-Maf KO
|
Fetal liver cells from E15.5 c-Maf+/+ and c-Maf-/- embryos were incubated with APC-conjugated anti-Mac-1 antibody (eBioscience) and PE-conjugated anti-F4/80 antibody (Serotec). Mac-1+F4/80+ cells were sorted by a FACSAria cell sorter (Becton Dickinson) as fetal liver macrophages.
|
| Sample_geo_accession | GSM571898
| | Sample_status | Public on Jul 06 2011
| | Sample_submission_date | Jul 29 2010
| | Sample_last_update_date | Jul 06 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | None.
| | Sample_growth_protocol_ch1 | None.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from fetal liver macrophages using a QIAGEN RNeasy Mini Kit according to the manufacturer’s instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | The microarray procedure was performed as described in the Affymetrix GeneChip Expression Analysis Manual (Affymetrix).
| | Sample_hyb_protocol | Biotin-labeled cRNA was synthesized from 4 to 6 μg RNA and hybridized to a GeneChip Mouse Genome 430 2.0 Array (Affymetrix).
| | Sample_scan_protocol | After washing, arrays were stained with streptavidin-phycoethrin and analysed on a scanner.
| | Sample_data_processing | The data processing has been performed according to the manufacture's protocol (Affymetrix). Affymetrix Algorithm: ExpressionStat 5.0
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Manabu,,Kusakabe
| | Sample_contact_phone | 81-29-853-6963
| | Sample_contact_fax | 81-29-853-6965
| | Sample_contact_laboratory | Takahashi Laboratory
| | Sample_contact_department | Department of Anatomy and Embryology
| | Sample_contact_institute | University of Tsukuba
| | Sample_contact_address | 1-1-1, Tennodai,
| | Sample_contact_city | Tsukuba
| | Sample_contact_state | IBARAKI
| | Sample_contact_zip/postal_code | 305-8575
| | Sample_contact_country | Japan
| | Sample_contact_web_link | http://www.md.tsukuba.ac.jp/basic-med/anatomy/embryology/index_e.html
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM571nnn/GSM571898/suppl/GSM571898.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM571nnn/GSM571898/suppl/GSM571898.CHP.gz
| | Sample_series_id | GSE23305
| | Sample_data_row_count | 45101
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