Search results for the GEO ID: GSE23391 |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM573678 | GPL570 |
|
mRNA CEPI 489
|
Laser Capture Microdissected Serous Ovarian Cancer Epithelia
|
tumor stage/grade: IV/3
age at surgery: 48
|
Serous ovarian tumor tissues were placed in cryotubes and immediately (<1 minute) frozen in liquid nitrogen following resection.
|
Sample_geo_accession | GSM573678
| Sample_status | Public on Jul 29 2011
| Sample_submission_date | Aug 03 2010
| Sample_last_update_date | Jul 29 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Following pathological verification, a serous papillary epithelial cancer sample was embedded in cryomatrix (Shandon). Seven micron frozen sections were cut and attached to uncharged microscope slides for each sample. Immediately following dehydration and staining (HistoGene, LCM Frozen Section Staining Kit, Arcturus), slides were processed in an Autopix‘ instrument (Arcturus) for laser capture microdissection (LCM) of cells to CapSure Macro LCM Caps. Approximately 30,000 epithelia were collected from each cancer sample.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA was isolated with Trizol (Invitrogen) and purified with PicoPure RNA Isolation Kit (Arcturus). RNA quality was verified on the Bioanalyzer RNA Pico Chip (Agilent Technologies).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Total RNA from the above extractions was processed using the RiboAmp OA or HS kit (Arcturus) in conjunction with the IVT Labeling Kit from Affymetrix, to produce an amplified, biotin-labeled mRNA suitable for hybridizing to GeneChip Probe Arrays (Affymetrix).
| Sample_hyb_protocol | Labeled mRNA was hybridized to GeneChip Human Genome U133 Plus 2.0 Arrays in the GeneChip Hybridization oven 640, further processed with the GeneChip Fluidics Station 450 and scanned with the GeneChip Scanner 3000.
| Sample_scan_protocol | as per manufacturer's protocol using GeneChip Scanner 3000.
| Sample_data_processing | MAS 5.0 via Affymetrix Expression Console (TM) Software Version 1.1
| Sample_platform_id | GPL570
| Sample_contact_name | Shubin,,Shahab
| Sample_contact_email | shubin.shahab@gmail.com
| Sample_contact_laboratory | McDonald
| Sample_contact_department | Biology
| Sample_contact_institute | Georgia Institute of Technology
| Sample_contact_address | 310 Ferst Drive
| Sample_contact_city | Atlanta
| Sample_contact_state | GA
| Sample_contact_zip/postal_code | 30332
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM573nnn/GSM573678/suppl/GSM573678.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM573nnn/GSM573678/suppl/GSM573678.CHP.gz
| Sample_series_id | GSE23391
| Sample_series_id | GSE23392
| Sample_data_row_count | 54675
| |
|
GSM573679 | GPL570 |
|
mRNA CEPI 551
|
Laser Capture Microdissected Serous Ovarian Cancer Epithelia
|
tumor stage/grade: IIIc/IV/3
age at surgery: 59
|
Serous ovarian tumor tissues were placed in cryotubes and immediately (<1 minute) frozen in liquid nitrogen following resection.
|
Sample_geo_accession | GSM573679
| Sample_status | Public on Jul 29 2011
| Sample_submission_date | Aug 03 2010
| Sample_last_update_date | Jul 29 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Following pathological verification, a serous papillary epithelial cancer sample was embedded in cryomatrix (Shandon). Seven micron frozen sections were cut and attached to uncharged microscope slides for each sample. Immediately following dehydration and staining (HistoGene, LCM Frozen Section Staining Kit, Arcturus), slides were processed in an Autopix‘ instrument (Arcturus) for laser capture microdissection (LCM) of cells to CapSure Macro LCM Caps. Approximately 30,000 epithelia were collected from each cancer sample.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA was isolated with Trizol (Invitrogen) and purified with PicoPure RNA Isolation Kit (Arcturus). RNA quality was verified on the Bioanalyzer RNA Pico Chip (Agilent Technologies).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Total RNA from the above extractions was processed using the RiboAmp OA or HS kit (Arcturus) in conjunction with the IVT Labeling Kit from Affymetrix, to produce an amplified, biotin-labeled mRNA suitable for hybridizing to GeneChip Probe Arrays (Affymetrix).
| Sample_hyb_protocol | Labeled mRNA was hybridized to GeneChip Human Genome U133 Plus 2.0 Arrays in the GeneChip Hybridization oven 640, further processed with the GeneChip Fluidics Station 450 and scanned with the GeneChip Scanner 3000.
| Sample_scan_protocol | as per manufacturer's protocol using GeneChip Scanner 3000.
| Sample_data_processing | MAS 5.0 via Affymetrix Expression Console (TM) Software Version 1.1
| Sample_platform_id | GPL570
| Sample_contact_name | Shubin,,Shahab
| Sample_contact_email | shubin.shahab@gmail.com
| Sample_contact_laboratory | McDonald
| Sample_contact_department | Biology
| Sample_contact_institute | Georgia Institute of Technology
| Sample_contact_address | 310 Ferst Drive
| Sample_contact_city | Atlanta
| Sample_contact_state | GA
| Sample_contact_zip/postal_code | 30332
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM573nnn/GSM573679/suppl/GSM573679.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM573nnn/GSM573679/suppl/GSM573679.CHP.gz
| Sample_series_id | GSE23391
| Sample_series_id | GSE23392
| Sample_data_row_count | 54675
| |
|
GSM573680 | GPL570 |
|
mRNA CEPI 588
|
Laser Capture Microdissected Serous Ovarian Cancer Epithelia
|
tumor stage/grade: IIIc/2-3
age at surgery: 71
|
Serous ovarian tumor tissues were placed in cryotubes and immediately (<1 minute) frozen in liquid nitrogen following resection.
|
Sample_geo_accession | GSM573680
| Sample_status | Public on Jul 29 2011
| Sample_submission_date | Aug 03 2010
| Sample_last_update_date | Jul 29 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Following pathological verification, a serous papillary epithelial cancer sample was embedded in cryomatrix (Shandon). Seven micron frozen sections were cut and attached to uncharged microscope slides for each sample. Immediately following dehydration and staining (HistoGene, LCM Frozen Section Staining Kit, Arcturus), slides were processed in an Autopix‘ instrument (Arcturus) for laser capture microdissection (LCM) of cells to CapSure Macro LCM Caps. Approximately 30,000 epithelia were collected from each cancer sample.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA was isolated with Trizol (Invitrogen) and purified with PicoPure RNA Isolation Kit (Arcturus). RNA quality was verified on the Bioanalyzer RNA Pico Chip (Agilent Technologies).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Total RNA from the above extractions was processed using the RiboAmp OA or HS kit (Arcturus) in conjunction with the IVT Labeling Kit from Affymetrix, to produce an amplified, biotin-labeled mRNA suitable for hybridizing to GeneChip Probe Arrays (Affymetrix).
| Sample_hyb_protocol | Labeled mRNA was hybridized to GeneChip Human Genome U133 Plus 2.0 Arrays in the GeneChip Hybridization oven 640, further processed with the GeneChip Fluidics Station 450 and scanned with the GeneChip Scanner 3000.
| Sample_scan_protocol | as per manufacturer's protocol using GeneChip Scanner 3000.
| Sample_data_processing | MAS 5.0 via Affymetrix Expression Console (TM) Software Version 1.1
| Sample_platform_id | GPL570
| Sample_contact_name | Shubin,,Shahab
| Sample_contact_email | shubin.shahab@gmail.com
| Sample_contact_laboratory | McDonald
| Sample_contact_department | Biology
| Sample_contact_institute | Georgia Institute of Technology
| Sample_contact_address | 310 Ferst Drive
| Sample_contact_city | Atlanta
| Sample_contact_state | GA
| Sample_contact_zip/postal_code | 30332
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM573nnn/GSM573680/suppl/GSM573680.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM573nnn/GSM573680/suppl/GSM573680.CHP.gz
| Sample_series_id | GSE23391
| Sample_series_id | GSE23392
| Sample_data_row_count | 54675
| |
|
GSM573681 | GPL570 |
|
mRNA OSE 434
|
Ovarian Surface Epithelia
|
tumor stage/grade: N/A
age at surgery: 41
|
Ovarian surface epithelia brushing from ovaries described by pathology as within normal limits.
|
Sample_geo_accession | GSM573681
| Sample_status | Public on Jul 29 2011
| Sample_submission_date | Aug 03 2010
| Sample_last_update_date | Jul 29 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Normal ovarian surface epithelial (OSE) cells were collected from ovaries at time of surgery using a Cytobrush ® Plus (Medscand) and immediately stabilized in RNAlater (Ambion) and stored at –20 degrees C.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA was isolated with Trizol (Invitrogen) and purified with PicoPure RNA Isolation Kit (Arcturus). RNA quality was verified on the Bioanalyzer RNA Pico Chip (Agilent Technologies).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Affymetrix IVT Labeling Kit
| Sample_hyb_protocol | Labeled mRNA was hybridized to GeneChip Human Genome U133 Plus 2.0 Arrays in the GeneChip Hybridization oven 640, further processed with the GeneChip Fluidics Station 450 and scanned with the GeneChip Scanner 3000.
| Sample_scan_protocol | as per manufacturer's protocol using GeneChip Scanner 3000.
| Sample_data_processing | MAS 5.0 via Affymetrix Expression Console (TM) Software Version 1.1
| Sample_platform_id | GPL570
| Sample_contact_name | Shubin,,Shahab
| Sample_contact_email | shubin.shahab@gmail.com
| Sample_contact_laboratory | McDonald
| Sample_contact_department | Biology
| Sample_contact_institute | Georgia Institute of Technology
| Sample_contact_address | 310 Ferst Drive
| Sample_contact_city | Atlanta
| Sample_contact_state | GA
| Sample_contact_zip/postal_code | 30332
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM573nnn/GSM573681/suppl/GSM573681.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM573nnn/GSM573681/suppl/GSM573681.CHP.gz
| Sample_series_id | GSE23391
| Sample_series_id | GSE23392
| Sample_data_row_count | 54675
| |
|
GSM573682 | GPL570 |
|
miRNA OSE 437
|
Ovarian Surface Epithelia
|
tumor stage/grade: N/A
age at surgery: 54
|
Ovarian surface epithelia brushing from ovaries described by pathology as within normal limits.
|
Sample_geo_accession | GSM573682
| Sample_status | Public on Jul 29 2011
| Sample_submission_date | Aug 03 2010
| Sample_last_update_date | Jul 29 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Normal ovarian surface epithelial (OSE) cells were collected from ovaries at time of surgery using a Cytobrush ® Plus (Medscand) and immediately stabilized in RNAlater (Ambion) and stored at –20 degrees C.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA was isolated with Trizol (Invitrogen) and purified with PicoPure RNA Isolation Kit (Arcturus). RNA quality was verified on the Bioanalyzer RNA Pico Chip (Agilent Technologies).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Affymetrix IVT Labeling Kit
| Sample_hyb_protocol | Labeled mRNA was hybridized to GeneChip Human Genome U133 Plus 2.0 Arrays in the GeneChip Hybridization oven 640, further processed with the GeneChip Fluidics Station 450 and scanned with the GeneChip Scanner 3000.
| Sample_scan_protocol | as per manufacturer's protocol using GeneChip Scanner 3000.
| Sample_data_processing | MAS 5.0 via Affymetrix Expression Console (TM) Software Version 1.1
| Sample_platform_id | GPL570
| Sample_contact_name | Shubin,,Shahab
| Sample_contact_email | shubin.shahab@gmail.com
| Sample_contact_laboratory | McDonald
| Sample_contact_department | Biology
| Sample_contact_institute | Georgia Institute of Technology
| Sample_contact_address | 310 Ferst Drive
| Sample_contact_city | Atlanta
| Sample_contact_state | GA
| Sample_contact_zip/postal_code | 30332
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM573nnn/GSM573682/suppl/GSM573682.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM573nnn/GSM573682/suppl/GSM573682.CHP.gz
| Sample_series_id | GSE23391
| Sample_series_id | GSE23392
| Sample_data_row_count | 54675
| |
|
GSM573683 | GPL570 |
|
miRNA OSE 440
|
Ovarian Surface Epithelia
|
tumor stage/grade: N/A
age at surgery: 50
|
Ovarian surface epithelia brushing from ovaries described by pathology as within normal limits.
|
Sample_geo_accession | GSM573683
| Sample_status | Public on Jul 29 2011
| Sample_submission_date | Aug 03 2010
| Sample_last_update_date | Jul 29 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Normal ovarian surface epithelial (OSE) cells were collected from ovaries at time of surgery using a Cytobrush ® Plus (Medscand) and immediately stabilized in RNAlater (Ambion) and stored at –20 degrees C.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA was isolated with Trizol (Invitrogen) and purified with PicoPure RNA Isolation Kit (Arcturus). RNA quality was verified on the Bioanalyzer RNA Pico Chip (Agilent Technologies).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Affymetrix IVT Labeling Kit
| Sample_hyb_protocol | Labeled mRNA was hybridized to GeneChip Human Genome U133 Plus 2.0 Arrays in the GeneChip Hybridization oven 640, further processed with the GeneChip Fluidics Station 450 and scanned with the GeneChip Scanner 3000.
| Sample_scan_protocol | as per manufacturer's protocol using GeneChip Scanner 3000.
| Sample_data_processing | MAS 5.0 via Affymetrix Expression Console (TM) Software Version 1.1
| Sample_platform_id | GPL570
| Sample_contact_name | Shubin,,Shahab
| Sample_contact_email | shubin.shahab@gmail.com
| Sample_contact_laboratory | McDonald
| Sample_contact_department | Biology
| Sample_contact_institute | Georgia Institute of Technology
| Sample_contact_address | 310 Ferst Drive
| Sample_contact_city | Atlanta
| Sample_contact_state | GA
| Sample_contact_zip/postal_code | 30332
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM573nnn/GSM573683/suppl/GSM573683.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM573nnn/GSM573683/suppl/GSM573683.CHP.gz
| Sample_series_id | GSE23391
| Sample_series_id | GSE23392
| Sample_data_row_count | 54675
| |
|
GSM573684 | GPL570 |
|
miRNA OSE 470
|
Ovarian Surface Epithelia
|
tumor stage/grade: N/A
age at surgery: 44
|
Ovarian surface epithelia brushing from ovaries described by pathology as within normal limits.
|
Sample_geo_accession | GSM573684
| Sample_status | Public on Jul 29 2011
| Sample_submission_date | Aug 03 2010
| Sample_last_update_date | Jul 29 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Normal ovarian surface epithelial (OSE) cells were collected from ovaries at time of surgery using a Cytobrush ® Plus (Medscand) and immediately stabilized in RNAlater (Ambion) and stored at –20 degrees C.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA was isolated with Trizol (Invitrogen) and purified with PicoPure RNA Isolation Kit (Arcturus). RNA quality was verified on the Bioanalyzer RNA Pico Chip (Agilent Technologies).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Affymetrix IVT Labeling Kit
| Sample_hyb_protocol | Labeled mRNA was hybridized to GeneChip Human Genome U133 Plus 2.0 Arrays in the GeneChip Hybridization oven 640, further processed with the GeneChip Fluidics Station 450 and scanned with the GeneChip Scanner 3000.
| Sample_scan_protocol | as per manufacturer's protocol using GeneChip Scanner 3000.
| Sample_data_processing | MAS 5.0 via Affymetrix Expression Console (TM) Software Version 1.1
| Sample_platform_id | GPL570
| Sample_contact_name | Shubin,,Shahab
| Sample_contact_email | shubin.shahab@gmail.com
| Sample_contact_laboratory | McDonald
| Sample_contact_department | Biology
| Sample_contact_institute | Georgia Institute of Technology
| Sample_contact_address | 310 Ferst Drive
| Sample_contact_city | Atlanta
| Sample_contact_state | GA
| Sample_contact_zip/postal_code | 30332
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM573nnn/GSM573684/suppl/GSM573684.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM573nnn/GSM573684/suppl/GSM573684.CHP.gz
| Sample_series_id | GSE23391
| Sample_series_id | GSE23392
| Sample_data_row_count | 54675
| |
|
GSM573685 | GPL570 |
|
miRNA OSE 475
|
Ovarian Surface Epithelia
|
tumor stage/grade: N/A
age at surgery: 63
|
Ovarian surface epithelia brushing from ovaries described by pathology as within normal limits.
|
Sample_geo_accession | GSM573685
| Sample_status | Public on Jul 29 2011
| Sample_submission_date | Aug 03 2010
| Sample_last_update_date | Jul 29 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Normal ovarian surface epithelial (OSE) cells were collected from ovaries at time of surgery using a Cytobrush ® Plus (Medscand) and immediately stabilized in RNAlater (Ambion) and stored at –20 degrees C.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNA was isolated with Trizol (Invitrogen) and purified with PicoPure RNA Isolation Kit (Arcturus). RNA quality was verified on the Bioanalyzer RNA Pico Chip (Agilent Technologies).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Affymetrix IVT Labeling Kit
| Sample_hyb_protocol | Labeled mRNA was hybridized to GeneChip Human Genome U133 Plus 2.0 Arrays in the GeneChip Hybridization oven 640, further processed with the GeneChip Fluidics Station 450 and scanned with the GeneChip Scanner 3000.
| Sample_scan_protocol | as per manufacturer's protocol using GeneChip Scanner 3000.
| Sample_data_processing | MAS 5.0 via Affymetrix Expression Console (TM) Software Version 1.1
| Sample_platform_id | GPL570
| Sample_contact_name | Shubin,,Shahab
| Sample_contact_email | shubin.shahab@gmail.com
| Sample_contact_laboratory | McDonald
| Sample_contact_department | Biology
| Sample_contact_institute | Georgia Institute of Technology
| Sample_contact_address | 310 Ferst Drive
| Sample_contact_city | Atlanta
| Sample_contact_state | GA
| Sample_contact_zip/postal_code | 30332
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM573nnn/GSM573685/suppl/GSM573685.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM573nnn/GSM573685/suppl/GSM573685.CHP.gz
| Sample_series_id | GSE23391
| Sample_series_id | GSE23392
| Sample_data_row_count | 54675
| |
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