Search results for the GEO ID: GSE23434  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM575183 | GPL570 |
|
HK2 cells at T0, biological replicate 1
|
HK2 (Human Kidney 2) cell line from ATCC at 8th generation.
|
cell type: human kidney
cell line: HK2 from ATCC
cell passage: 8th generation
sex: male
developmental stage: adult
treatment group: T0
|
Gene expression data from HK2 cell lines before PCB153 treatment.
|
| Sample_geo_accession | GSM575183
| | Sample_status | Public on Aug 03 2012
| | Sample_submission_date | Aug 05 2010
| | Sample_last_update_date | Aug 03 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Treated with 70uM PCB153 for 0 hours
| | Sample_growth_protocol_ch1 | Grown for 48 hours in Keratinocyte-Serum Free Medium supplemented 5ng/ml recombinant epidermal growth factor and 0.05mg/ml bovinepituitary extract and 1X Pennicilllin-Streptromycin
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 microg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 microg of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneArray Scanner 3000.
| | Sample_data_processing | The data were analyzed using GeneSpring GX 10.0 with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 150.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Sisir,K.,Dutta
| | Sample_contact_email | sdutta@howard.edu
| | Sample_contact_phone | 202-806-6942
| | Sample_contact_fax | 202-806-5832
| | Sample_contact_laboratory | Molecular Genetics
| | Sample_contact_department | Biology
| | Sample_contact_institute | Howard University
| | Sample_contact_address | 415 College Street, NW
| | Sample_contact_city | Washington DC
| | Sample_contact_state | DC
| | Sample_contact_zip/postal_code | 20059
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM575nnn/GSM575183/suppl/GSM575183.CEL.gz
| | Sample_series_id | GSE23434
| | Sample_series_id | GSE23494
| | Sample_data_row_count | 54675
| | |
|
GSM575184 | GPL570 |
|
HK2 cells at T0, biological replicate 2
|
HK2 (Human Kidney 2) cell line from ATCC at 8th generation.
|
cell type: human kidney
cell line: HK2 from ATCC
cell passage: 8th generation
sex: male
developmental stage: adult
treatment group: T0
|
Gene expression data from HK2 cell lines before PCB153 treatment.
|
| Sample_geo_accession | GSM575184
| | Sample_status | Public on Aug 03 2012
| | Sample_submission_date | Aug 05 2010
| | Sample_last_update_date | Aug 03 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Treated with 70uM PCB153 for 0 hours
| | Sample_growth_protocol_ch1 | Grown for 48 hours in Keratinocyte-Serum Free Medium supplemented 5ng/ml recombinant epidermal growth factor and 0.05mg/ml bovinepituitary extract and 1X Pennicilllin-Streptromycin
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 microg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 microg of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneArray Scanner 3000.
| | Sample_data_processing | The data were analyzed using GeneSpring GX 10.0 with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 150.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Sisir,K.,Dutta
| | Sample_contact_email | sdutta@howard.edu
| | Sample_contact_phone | 202-806-6942
| | Sample_contact_fax | 202-806-5832
| | Sample_contact_laboratory | Molecular Genetics
| | Sample_contact_department | Biology
| | Sample_contact_institute | Howard University
| | Sample_contact_address | 415 College Street, NW
| | Sample_contact_city | Washington DC
| | Sample_contact_state | DC
| | Sample_contact_zip/postal_code | 20059
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM575nnn/GSM575184/suppl/GSM575184.CEL.gz
| | Sample_series_id | GSE23434
| | Sample_series_id | GSE23494
| | Sample_data_row_count | 54675
| | |
|
GSM575185 | GPL570 |
|
HK2 cells at T0, biological replicate 3
|
HK2 (Human Kidney 2) cell line from ATCC at 8th generation.
|
cell type: human kidney
cell line: HK2 from ATCC
cell passage: 8th generation
sex: male
developmental stage: adult
treatment group: T0
|
Gene expression data from HK2 cell lines before PCB153 treatment.
|
| Sample_geo_accession | GSM575185
| | Sample_status | Public on Aug 03 2012
| | Sample_submission_date | Aug 05 2010
| | Sample_last_update_date | Aug 03 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Treated with 70uM PCB153 for 0 hours
| | Sample_growth_protocol_ch1 | Grown for 48 hours in Keratinocyte-Serum Free Medium supplemented 5ng/ml recombinant epidermal growth factor and 0.05mg/ml bovinepituitary extract and 1X Pennicilllin-Streptromycin
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 microg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 microg of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneArray Scanner 3000.
| | Sample_data_processing | The data were analyzed using GeneSpring GX 10.0 with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 150.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Sisir,K.,Dutta
| | Sample_contact_email | sdutta@howard.edu
| | Sample_contact_phone | 202-806-6942
| | Sample_contact_fax | 202-806-5832
| | Sample_contact_laboratory | Molecular Genetics
| | Sample_contact_department | Biology
| | Sample_contact_institute | Howard University
| | Sample_contact_address | 415 College Street, NW
| | Sample_contact_city | Washington DC
| | Sample_contact_state | DC
| | Sample_contact_zip/postal_code | 20059
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM575nnn/GSM575185/suppl/GSM575185.CEL.gz
| | Sample_series_id | GSE23434
| | Sample_series_id | GSE23494
| | Sample_data_row_count | 54675
| | |
|
GSM575186 | GPL570 |
|
HK2 cells at T0.5, biological replicate 1
|
HK2 (Human Kidney 2) cell line from ATCC at 8th generation.
|
cell type: human kidney
cell line: HK2 from ATCC
cell passage: 8th generation
sex: male
developmental stage: adult
treatment group: T0.5
|
Gene expression data from HK2 cell lines after 30 minutes of PCB153 treatment.
|
| Sample_geo_accession | GSM575186
| | Sample_status | Public on Aug 03 2012
| | Sample_submission_date | Aug 05 2010
| | Sample_last_update_date | Aug 03 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Treated with 70uM PCB153 for 30 minutes
| | Sample_growth_protocol_ch1 | Grown for 48 hours in Keratinocyte-Serum Free Medium supplemented 5ng/ml recombinant epidermal growth factor and 0.05mg/ml bovinepituitary extract and 1X Pennicilllin-Streptromycin
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 microg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 microg of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneArray Scanner 3000.
| | Sample_data_processing | The data were analyzed using GeneSpring GX 10.0 with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 150.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Sisir,K.,Dutta
| | Sample_contact_email | sdutta@howard.edu
| | Sample_contact_phone | 202-806-6942
| | Sample_contact_fax | 202-806-5832
| | Sample_contact_laboratory | Molecular Genetics
| | Sample_contact_department | Biology
| | Sample_contact_institute | Howard University
| | Sample_contact_address | 415 College Street, NW
| | Sample_contact_city | Washington DC
| | Sample_contact_state | DC
| | Sample_contact_zip/postal_code | 20059
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM575nnn/GSM575186/suppl/GSM575186.CEL.gz
| | Sample_series_id | GSE23434
| | Sample_series_id | GSE23494
| | Sample_data_row_count | 54675
| | |
|
GSM575187 | GPL570 |
|
HK2 cells at T0.5, biological replicate 2
|
HK2 (Human Kidney 2) cell line from ATCC at 8th generation.
|
cell type: human kidney
cell line: HK2 from ATCC
cell passage: 8th generation
sex: male
developmental stage: adult
treatment group: T0.5
|
Gene expression data from HK2 cell lines after 30 minutes of PCB153 treatment.
|
| Sample_geo_accession | GSM575187
| | Sample_status | Public on Aug 03 2012
| | Sample_submission_date | Aug 05 2010
| | Sample_last_update_date | Aug 03 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Treated with 70uM PCB153 for 30 minutes
| | Sample_growth_protocol_ch1 | Grown for 48 hours in Keratinocyte-Serum Free Medium supplemented 5ng/ml recombinant epidermal growth factor and 0.05mg/ml bovinepituitary extract and 1X Pennicilllin-Streptromycin
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 microg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 microg of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneArray Scanner 3000.
| | Sample_data_processing | The data were analyzed using GeneSpring GX 10.0 with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 150.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Sisir,K.,Dutta
| | Sample_contact_email | sdutta@howard.edu
| | Sample_contact_phone | 202-806-6942
| | Sample_contact_fax | 202-806-5832
| | Sample_contact_laboratory | Molecular Genetics
| | Sample_contact_department | Biology
| | Sample_contact_institute | Howard University
| | Sample_contact_address | 415 College Street, NW
| | Sample_contact_city | Washington DC
| | Sample_contact_state | DC
| | Sample_contact_zip/postal_code | 20059
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM575nnn/GSM575187/suppl/GSM575187.CEL.gz
| | Sample_series_id | GSE23434
| | Sample_series_id | GSE23494
| | Sample_data_row_count | 54675
| | |
|
GSM575188 | GPL570 |
|
HK2 cells at T0.5, biological replicate 3
|
HK2 (Human Kidney 2) cell line from ATCC at 8th generation.
|
cell type: human kidney
cell line: HK2 from ATCC
cell passage: 8th generation
sex: male
developmental stage: adult
treatment group: T0.5
|
Gene expression data from HK2 cell lines after 30 minutes of PCB153 treatment.
|
| Sample_geo_accession | GSM575188
| | Sample_status | Public on Aug 03 2012
| | Sample_submission_date | Aug 05 2010
| | Sample_last_update_date | Aug 03 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Treated with 70uM PCB153 for 30 minutes
| | Sample_growth_protocol_ch1 | Grown for 48 hours in Keratinocyte-Serum Free Medium supplemented 5ng/ml recombinant epidermal growth factor and 0.05mg/ml bovinepituitary extract and 1X Pennicilllin-Streptromycin
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 microg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 microg of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneArray Scanner 3000.
| | Sample_data_processing | The data were analyzed using GeneSpring GX 10.0 with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 150.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Sisir,K.,Dutta
| | Sample_contact_email | sdutta@howard.edu
| | Sample_contact_phone | 202-806-6942
| | Sample_contact_fax | 202-806-5832
| | Sample_contact_laboratory | Molecular Genetics
| | Sample_contact_department | Biology
| | Sample_contact_institute | Howard University
| | Sample_contact_address | 415 College Street, NW
| | Sample_contact_city | Washington DC
| | Sample_contact_state | DC
| | Sample_contact_zip/postal_code | 20059
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM575nnn/GSM575188/suppl/GSM575188.CEL.gz
| | Sample_series_id | GSE23434
| | Sample_series_id | GSE23494
| | Sample_data_row_count | 54675
| | |
|
GSM575189 | GPL570 |
|
HK2 cells at T6, biological replicate 1
|
HK2 (Human Kidney 2) cell line from ATCC at 8th generation.
|
cell type: human kidney
cell line: HK2 from ATCC
cell passage: 8th generation
sex: male
developmental stage: adult
treatment group: T6
|
Gene expression data from HK2 cell lines after 6 hours of PCB153 treatment.
|
| Sample_geo_accession | GSM575189
| | Sample_status | Public on Aug 03 2012
| | Sample_submission_date | Aug 05 2010
| | Sample_last_update_date | Aug 03 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Treated with 70uM PCB153 for 6 hours
| | Sample_growth_protocol_ch1 | Grown for 48 hours in Keratinocyte-Serum Free Medium supplemented 5ng/ml recombinant epidermal growth factor and 0.05mg/ml bovinepituitary extract and 1X Pennicilllin-Streptromycin
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 microg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 microg of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneArray Scanner 3000.
| | Sample_data_processing | The data were analyzed using GeneSpring GX 10.0 with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 150.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Sisir,K.,Dutta
| | Sample_contact_email | sdutta@howard.edu
| | Sample_contact_phone | 202-806-6942
| | Sample_contact_fax | 202-806-5832
| | Sample_contact_laboratory | Molecular Genetics
| | Sample_contact_department | Biology
| | Sample_contact_institute | Howard University
| | Sample_contact_address | 415 College Street, NW
| | Sample_contact_city | Washington DC
| | Sample_contact_state | DC
| | Sample_contact_zip/postal_code | 20059
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM575nnn/GSM575189/suppl/GSM575189.CEL.gz
| | Sample_series_id | GSE23434
| | Sample_series_id | GSE23494
| | Sample_data_row_count | 54675
| | |
|
GSM575190 | GPL570 |
|
HK2 cells at T6, biological replicate 2
|
HK2 (Human Kidney 2) cell line from ATCC at 8th generation.
|
cell type: human kidney
cell line: HK2 from ATCC
cell passage: 8th generation
sex: male
developmental stage: adult
treatment group: T6
|
Gene expression data from HK2 cell lines after 6 hours of PCB153 treatment.
|
| Sample_geo_accession | GSM575190
| | Sample_status | Public on Aug 03 2012
| | Sample_submission_date | Aug 05 2010
| | Sample_last_update_date | Aug 03 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Treated with 70uM PCB153 for 6 hours
| | Sample_growth_protocol_ch1 | Grown for 48 hours in Keratinocyte-Serum Free Medium supplemented 5ng/ml recombinant epidermal growth factor and 0.05mg/ml bovinepituitary extract and 1X Pennicilllin-Streptromycin
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 microg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 microg of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneArray Scanner 3000.
| | Sample_data_processing | The data were analyzed using GeneSpring GX 10.0 with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 150.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Sisir,K.,Dutta
| | Sample_contact_email | sdutta@howard.edu
| | Sample_contact_phone | 202-806-6942
| | Sample_contact_fax | 202-806-5832
| | Sample_contact_laboratory | Molecular Genetics
| | Sample_contact_department | Biology
| | Sample_contact_institute | Howard University
| | Sample_contact_address | 415 College Street, NW
| | Sample_contact_city | Washington DC
| | Sample_contact_state | DC
| | Sample_contact_zip/postal_code | 20059
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM575nnn/GSM575190/suppl/GSM575190.CEL.gz
| | Sample_series_id | GSE23434
| | Sample_series_id | GSE23494
| | Sample_data_row_count | 54675
| | |
|
GSM575191 | GPL570 |
|
HK2 cells at T6, biological replicate 3
|
HK2 (Human Kidney 2) cell line from ATCC at 8th generation.
|
cell type: human kidney
cell line: HK2 from ATCC
cell passage: 8th generation
sex: male
developmental stage: adult
treatment group: T6
|
Gene expression data from HK2 cell lines after 6 hours of PCB153 treatment.
|
| Sample_geo_accession | GSM575191
| | Sample_status | Public on Aug 03 2012
| | Sample_submission_date | Aug 05 2010
| | Sample_last_update_date | Aug 03 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Treated with 70uM PCB153 for 6 hours
| | Sample_growth_protocol_ch1 | Grown for 48 hours in Keratinocyte-Serum Free Medium supplemented 5ng/ml recombinant epidermal growth factor and 0.05mg/ml bovinepituitary extract and 1X Pennicilllin-Streptromycin
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 microg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 microg of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneArray Scanner 3000.
| | Sample_data_processing | The data were analyzed using GeneSpring GX 10.0 with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 150.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Sisir,K.,Dutta
| | Sample_contact_email | sdutta@howard.edu
| | Sample_contact_phone | 202-806-6942
| | Sample_contact_fax | 202-806-5832
| | Sample_contact_laboratory | Molecular Genetics
| | Sample_contact_department | Biology
| | Sample_contact_institute | Howard University
| | Sample_contact_address | 415 College Street, NW
| | Sample_contact_city | Washington DC
| | Sample_contact_state | DC
| | Sample_contact_zip/postal_code | 20059
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM575nnn/GSM575191/suppl/GSM575191.CEL.gz
| | Sample_series_id | GSE23434
| | Sample_series_id | GSE23494
| | Sample_data_row_count | 54675
| | |
|
GSM575192 | GPL570 |
|
HK2 cells at T24, biological replicate 1
|
HK2 (Human Kidney 2) cell line from ATCC at 8th generation.
|
cell type: human kidney
cell line: HK2 from ATCC
cell passage: 8th generation
sex: male
developmental stage: adult
treatment group: T24
|
Gene expression data from HK2 cell lines after 24 hours of PCB153 treatment.
|
| Sample_geo_accession | GSM575192
| | Sample_status | Public on Aug 03 2012
| | Sample_submission_date | Aug 05 2010
| | Sample_last_update_date | Aug 03 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Treated with 70uM PCB153 for 24 hours
| | Sample_growth_protocol_ch1 | Grown for 48 hours in Keratinocyte-Serum Free Medium supplemented 5ng/ml recombinant epidermal growth factor and 0.05mg/ml bovinepituitary extract and 1X Pennicilllin-Streptromycin
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 microg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 microg of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneArray Scanner 3000.
| | Sample_data_processing | The data were analyzed using GeneSpring GX 10.0 with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 150.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Sisir,K.,Dutta
| | Sample_contact_email | sdutta@howard.edu
| | Sample_contact_phone | 202-806-6942
| | Sample_contact_fax | 202-806-5832
| | Sample_contact_laboratory | Molecular Genetics
| | Sample_contact_department | Biology
| | Sample_contact_institute | Howard University
| | Sample_contact_address | 415 College Street, NW
| | Sample_contact_city | Washington DC
| | Sample_contact_state | DC
| | Sample_contact_zip/postal_code | 20059
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM575nnn/GSM575192/suppl/GSM575192.CEL.gz
| | Sample_series_id | GSE23434
| | Sample_series_id | GSE23494
| | Sample_data_row_count | 54675
| | |
|
GSM575193 | GPL570 |
|
HK2 cells at T24, biological replicate 2
|
HK2 (Human Kidney 2) cell line from ATCC at 8th generation.
|
cell type: human kidney
cell line: HK2 from ATCC
cell passage: 8th generation
sex: male
developmental stage: adult
treatment group: T24
|
Gene expression data from HK2 cell lines after 24 hours of PCB153 treatment.
|
| Sample_geo_accession | GSM575193
| | Sample_status | Public on Aug 03 2012
| | Sample_submission_date | Aug 05 2010
| | Sample_last_update_date | Aug 03 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Treated with 70uM PCB153 for 24 hours
| | Sample_growth_protocol_ch1 | Grown for 48 hours in Keratinocyte-Serum Free Medium supplemented 5ng/ml recombinant epidermal growth factor and 0.05mg/ml bovinepituitary extract and 1X Pennicilllin-Streptromycin
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 microg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 microg of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneArray Scanner 3000.
| | Sample_data_processing | The data were analyzed using GeneSpring GX 10.0 with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 150.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Sisir,K.,Dutta
| | Sample_contact_email | sdutta@howard.edu
| | Sample_contact_phone | 202-806-6942
| | Sample_contact_fax | 202-806-5832
| | Sample_contact_laboratory | Molecular Genetics
| | Sample_contact_department | Biology
| | Sample_contact_institute | Howard University
| | Sample_contact_address | 415 College Street, NW
| | Sample_contact_city | Washington DC
| | Sample_contact_state | DC
| | Sample_contact_zip/postal_code | 20059
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM575nnn/GSM575193/suppl/GSM575193.CEL.gz
| | Sample_series_id | GSE23434
| | Sample_series_id | GSE23494
| | Sample_data_row_count | 54675
| | |
|
GSM575194 | GPL570 |
|
HK2 cells at T24, biological replicate 3
|
HK2 (Human Kidney 2) cell line from ATCC at 8th generation.
|
cell type: human kidney
cell line: HK2 from ATCC
cell passage: 8th generation
sex: male
developmental stage: adult
treatment group: T24
|
Gene expression data from HK2 cell lines after 24 hours of PCB153 treatment.
|
| Sample_geo_accession | GSM575194
| | Sample_status | Public on Aug 03 2012
| | Sample_submission_date | Aug 05 2010
| | Sample_last_update_date | Aug 03 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Treated with 70uM PCB153 for 24 hours
| | Sample_growth_protocol_ch1 | Grown for 48 hours in Keratinocyte-Serum Free Medium supplemented 5ng/ml recombinant epidermal growth factor and 0.05mg/ml bovinepituitary extract and 1X Pennicilllin-Streptromycin
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 microg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 microg of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneArray Scanner 3000.
| | Sample_data_processing | The data were analyzed using GeneSpring GX 10.0 with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 150.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Sisir,K.,Dutta
| | Sample_contact_email | sdutta@howard.edu
| | Sample_contact_phone | 202-806-6942
| | Sample_contact_fax | 202-806-5832
| | Sample_contact_laboratory | Molecular Genetics
| | Sample_contact_department | Biology
| | Sample_contact_institute | Howard University
| | Sample_contact_address | 415 College Street, NW
| | Sample_contact_city | Washington DC
| | Sample_contact_state | DC
| | Sample_contact_zip/postal_code | 20059
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM575nnn/GSM575194/suppl/GSM575194.CEL.gz
| | Sample_series_id | GSE23434
| | Sample_series_id | GSE23494
| | Sample_data_row_count | 54675
| | |
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