Result

Search results for the GEO ID: GSE23437

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GSM ID

GPL ID

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Title

Source name

Description

Characteristics

GSM575233

GPL1261

Mouse retina_Control_ rep1

retina, no hyperoxia

strain: C57BL/6J tissue: Retina age: Adult P80 - 120

Gene expression data from mouse retina exposued to normal oxygen levels

Sample_geo_accession	GSM575233
Sample_status	Public on Aug 06 2010
Sample_submission_date	Aug 05 2010
Sample_last_update_date	Aug 05 2010
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Animals were placed in an oxygen chamber and exposed to 75% oxygen for a period of either 3, 7 or 14 days.
Sample_growth_protocol_ch1	Animals were raised in cyclic light conditions using 12hrs dim (5 lux) and 12hrs dark (no light).
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was extracted from each retinal biopsy using Trizol and chloroform to separate the RNA from other cell components. The RNA was then purified and isolated using RNAqueous-Micro RNA isolation kit (Ambion, Inc., Austin, TX). In some cases where genomic DNA contamination was a concern, the RNA was DNAse treated.
Sample_label_ch1	Biotin
Sample_label_protocol_ch1	Biotin-labeled cRNA was prepared according to the standard Affymetrix protocol from 500ng total RNA (Affymetrix manual, P/N 702646 Rev. 7).
Sample_hyb_protocol	Standard Affymetrix protocols were followed in the hybridisation, blocking and washing (GeneChip® Expression Analysis Technical Manual (P/N 702232 Rev. 3)).
Sample_scan_protocol	The arrays were scanned on the GCS 3000 Affymetrix high resolution scanner and analyzed using the GeneChip Operating Software v1.4 (GCOS; Affymetrix) and GeneSpring v 7.3.1 (Agilent Technologies).
Sample_data_processing	RMA normalization performed in GeneSpring v 7.3.1. Log2 RMA data was quantile transformed to median sample baseline.
Sample_platform_id	GPL1261
Sample_contact_name	Riccardo,,Natoli
Sample_contact_email	riccardo.natoli@anu.edu.au
Sample_contact_phone	+ 61 2 6125 8559
Sample_contact_fax	+ 61 2 6125 8680
Sample_contact_laboratory	Provis Lab
Sample_contact_department	CMBE
Sample_contact_institute	ANU
Sample_contact_address	Sullivans Creek Road
Sample_contact_city	Acton
Sample_contact_state	ACT
Sample_contact_zip/postal_code	2617
Sample_contact_country	Australia
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM575nnn/GSM575233/suppl/GSM575233.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM575nnn/GSM575233/suppl/GSM575233.CHP.gz
Sample_series_id	GSE23437
Sample_data_row_count	45101

GSM575234

GPL1261

Mouse retina_Control_ rep2

retina, no hyperoxia

strain: C57BL/6J tissue: Retina age: Adult P80 - 120

Gene expression data from mouse retina exposued to normal oxygen levels

Sample_geo_accession	GSM575234
Sample_status	Public on Aug 06 2010
Sample_submission_date	Aug 05 2010
Sample_last_update_date	Aug 05 2010
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Animals were placed in an oxygen chamber and exposed to 75% oxygen for a period of either 3, 7 or 14 days.
Sample_growth_protocol_ch1	Animals were raised in cyclic light conditions using 12hrs dim (5 lux) and 12hrs dark (no light).
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was extracted from each retinal biopsy using Trizol and chloroform to separate the RNA from other cell components. The RNA was then purified and isolated using RNAqueous-Micro RNA isolation kit (Ambion, Inc., Austin, TX). In some cases where genomic DNA contamination was a concern, the RNA was DNAse treated.
Sample_label_ch1	Biotin
Sample_label_protocol_ch1	Biotin-labeled cRNA was prepared according to the standard Affymetrix protocol from 500ng total RNA (Affymetrix manual, P/N 702646 Rev. 7).
Sample_hyb_protocol	Standard Affymetrix protocols were followed in the hybridisation, blocking and washing (GeneChip® Expression Analysis Technical Manual (P/N 702232 Rev. 3)).
Sample_scan_protocol	The arrays were scanned on the GCS 3000 Affymetrix high resolution scanner and analyzed using the GeneChip Operating Software v1.4 (GCOS; Affymetrix) and GeneSpring v 7.3.1 (Agilent Technologies).
Sample_data_processing	RMA normalization performed in GeneSpring v 7.3.1. Log2 RMA data was quantile transformed to median sample baseline.
Sample_platform_id	GPL1261
Sample_contact_name	Riccardo,,Natoli
Sample_contact_email	riccardo.natoli@anu.edu.au
Sample_contact_phone	+ 61 2 6125 8559
Sample_contact_fax	+ 61 2 6125 8680
Sample_contact_laboratory	Provis Lab
Sample_contact_department	CMBE
Sample_contact_institute	ANU
Sample_contact_address	Sullivans Creek Road
Sample_contact_city	Acton
Sample_contact_state	ACT
Sample_contact_zip/postal_code	2617
Sample_contact_country	Australia
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM575nnn/GSM575234/suppl/GSM575234.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM575nnn/GSM575234/suppl/GSM575234.CHP.gz
Sample_series_id	GSE23437
Sample_data_row_count	45101

GSM575235

GPL1261

Mouse retina_3 days_ rep1

retina, 3 days exposure to 75% hyperoxia

strain: C57BL/6J tissue: Retina age: Adult P80 - 120

Gene expression data from mouse retina exposued to 3 days 75% hyperoxia

Sample_geo_accession	GSM575235
Sample_status	Public on Aug 06 2010
Sample_submission_date	Aug 05 2010
Sample_last_update_date	Aug 05 2010
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Animals were placed in an oxygen chamber and exposed to 75% oxygen for a period of either 3, 7 or 14 days.
Sample_growth_protocol_ch1	Animals were raised in cyclic light conditions using 12hrs dim (5 lux) and 12hrs dark (no light).
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was extracted from each retinal biopsy using Trizol and chloroform to separate the RNA from other cell components. The RNA was then purified and isolated using RNAqueous-Micro RNA isolation kit (Ambion, Inc., Austin, TX). In some cases where genomic DNA contamination was a concern, the RNA was DNAse treated.
Sample_label_ch1	Biotin
Sample_label_protocol_ch1	Biotin-labeled cRNA was prepared according to the standard Affymetrix protocol from 500ng total RNA (Affymetrix manual, P/N 702646 Rev. 7).
Sample_hyb_protocol	Standard Affymetrix protocols were followed in the hybridisation, blocking and washing (GeneChip® Expression Analysis Technical Manual (P/N 702232 Rev. 3)).
Sample_scan_protocol	The arrays were scanned on the GCS 3000 Affymetrix high resolution scanner and analyzed using the GeneChip Operating Software v1.4 (GCOS; Affymetrix) and GeneSpring v 7.3.1 (Agilent Technologies).
Sample_data_processing	RMA normalization performed in GeneSpring v 7.3.1. Log2 RMA data was quantile transformed to median sample baseline.
Sample_platform_id	GPL1261
Sample_contact_name	Riccardo,,Natoli
Sample_contact_email	riccardo.natoli@anu.edu.au
Sample_contact_phone	+ 61 2 6125 8559
Sample_contact_fax	+ 61 2 6125 8680
Sample_contact_laboratory	Provis Lab
Sample_contact_department	CMBE
Sample_contact_institute	ANU
Sample_contact_address	Sullivans Creek Road
Sample_contact_city	Acton
Sample_contact_state	ACT
Sample_contact_zip/postal_code	2617
Sample_contact_country	Australia
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM575nnn/GSM575235/suppl/GSM575235.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM575nnn/GSM575235/suppl/GSM575235.CHP.gz
Sample_series_id	GSE23437
Sample_data_row_count	45101

GSM575236

GPL1261

Mouse retina_3 days_ rep2

retina, 3 days exposure to 75% hyperoxia

strain: C57BL/6J tissue: Retina age: Adult P80 - 120

Gene expression data from mouse retina exposued to 3 days 75% hyperoxia

Sample_geo_accession	GSM575236
Sample_status	Public on Aug 06 2010
Sample_submission_date	Aug 05 2010
Sample_last_update_date	Aug 05 2010
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Animals were placed in an oxygen chamber and exposed to 75% oxygen for a period of either 3, 7 or 14 days.
Sample_growth_protocol_ch1	Animals were raised in cyclic light conditions using 12hrs dim (5 lux) and 12hrs dark (no light).
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was extracted from each retinal biopsy using Trizol and chloroform to separate the RNA from other cell components. The RNA was then purified and isolated using RNAqueous-Micro RNA isolation kit (Ambion, Inc., Austin, TX). In some cases where genomic DNA contamination was a concern, the RNA was DNAse treated.
Sample_label_ch1	Biotin
Sample_label_protocol_ch1	Biotin-labeled cRNA was prepared according to the standard Affymetrix protocol from 500ng total RNA (Affymetrix manual, P/N 702646 Rev. 7).
Sample_hyb_protocol	Standard Affymetrix protocols were followed in the hybridisation, blocking and washing (GeneChip® Expression Analysis Technical Manual (P/N 702232 Rev. 3)).
Sample_scan_protocol	The arrays were scanned on the GCS 3000 Affymetrix high resolution scanner and analyzed using the GeneChip Operating Software v1.4 (GCOS; Affymetrix) and GeneSpring v 7.3.1 (Agilent Technologies).
Sample_data_processing	RMA normalization performed in GeneSpring v 7.3.1. Log2 RMA data was quantile transformed to median sample baseline.
Sample_platform_id	GPL1261
Sample_contact_name	Riccardo,,Natoli
Sample_contact_email	riccardo.natoli@anu.edu.au
Sample_contact_phone	+ 61 2 6125 8559
Sample_contact_fax	+ 61 2 6125 8680
Sample_contact_laboratory	Provis Lab
Sample_contact_department	CMBE
Sample_contact_institute	ANU
Sample_contact_address	Sullivans Creek Road
Sample_contact_city	Acton
Sample_contact_state	ACT
Sample_contact_zip/postal_code	2617
Sample_contact_country	Australia
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM575nnn/GSM575236/suppl/GSM575236.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM575nnn/GSM575236/suppl/GSM575236.CHP.gz
Sample_series_id	GSE23437
Sample_data_row_count	45101

GSM575237

GPL1261

Mouse retina_7 days_ rep1

retina, 7 days exposure to 75% hyperoxia

strain: C57BL/6J tissue: Retina age: Adult P80 - 120

Gene expression data from mouse retina exposued to 7 days 75% hyperoxia

Sample_geo_accession	GSM575237
Sample_status	Public on Aug 06 2010
Sample_submission_date	Aug 05 2010
Sample_last_update_date	Aug 05 2010
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Animals were placed in an oxygen chamber and exposed to 75% oxygen for a period of either 3, 7 or 14 days.
Sample_growth_protocol_ch1	Animals were raised in cyclic light conditions using 12hrs dim (5 lux) and 12hrs dark (no light).
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was extracted from each retinal biopsy using Trizol and chloroform to separate the RNA from other cell components. The RNA was then purified and isolated using RNAqueous-Micro RNA isolation kit (Ambion, Inc., Austin, TX). In some cases where genomic DNA contamination was a concern, the RNA was DNAse treated.
Sample_label_ch1	Biotin
Sample_label_protocol_ch1	Biotin-labeled cRNA was prepared according to the standard Affymetrix protocol from 500ng total RNA (Affymetrix manual, P/N 702646 Rev. 7).
Sample_hyb_protocol	Standard Affymetrix protocols were followed in the hybridisation, blocking and washing (GeneChip® Expression Analysis Technical Manual (P/N 702232 Rev. 3)).
Sample_scan_protocol	The arrays were scanned on the GCS 3000 Affymetrix high resolution scanner and analyzed using the GeneChip Operating Software v1.4 (GCOS; Affymetrix) and GeneSpring v 7.3.1 (Agilent Technologies).
Sample_data_processing	RMA normalization performed in GeneSpring v 7.3.1. Log2 RMA data was quantile transformed to median sample baseline.
Sample_platform_id	GPL1261
Sample_contact_name	Riccardo,,Natoli
Sample_contact_email	riccardo.natoli@anu.edu.au
Sample_contact_phone	+ 61 2 6125 8559
Sample_contact_fax	+ 61 2 6125 8680
Sample_contact_laboratory	Provis Lab
Sample_contact_department	CMBE
Sample_contact_institute	ANU
Sample_contact_address	Sullivans Creek Road
Sample_contact_city	Acton
Sample_contact_state	ACT
Sample_contact_zip/postal_code	2617
Sample_contact_country	Australia
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM575nnn/GSM575237/suppl/GSM575237.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM575nnn/GSM575237/suppl/GSM575237.CHP.gz
Sample_series_id	GSE23437
Sample_data_row_count	45101

GSM575238

GPL1261

Mouse retina_7 days_ rep2

retina, 7 days exposure to 75% hyperoxia

strain: C57BL/6J tissue: Retina age: Adult P80 - 120

Gene expression data from mouse retina exposued to 7 days 75% hyperoxia

Sample_geo_accession	GSM575238
Sample_status	Public on Aug 06 2010
Sample_submission_date	Aug 05 2010
Sample_last_update_date	Aug 05 2010
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Animals were placed in an oxygen chamber and exposed to 75% oxygen for a period of either 3, 7 or 14 days.
Sample_growth_protocol_ch1	Animals were raised in cyclic light conditions using 12hrs dim (5 lux) and 12hrs dark (no light).
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was extracted from each retinal biopsy using Trizol and chloroform to separate the RNA from other cell components. The RNA was then purified and isolated using RNAqueous-Micro RNA isolation kit (Ambion, Inc., Austin, TX). In some cases where genomic DNA contamination was a concern, the RNA was DNAse treated.
Sample_label_ch1	Biotin
Sample_label_protocol_ch1	Biotin-labeled cRNA was prepared according to the standard Affymetrix protocol from 500ng total RNA (Affymetrix manual, P/N 702646 Rev. 7).
Sample_hyb_protocol	Standard Affymetrix protocols were followed in the hybridisation, blocking and washing (GeneChip® Expression Analysis Technical Manual (P/N 702232 Rev. 3)).
Sample_scan_protocol	The arrays were scanned on the GCS 3000 Affymetrix high resolution scanner and analyzed using the GeneChip Operating Software v1.4 (GCOS; Affymetrix) and GeneSpring v 7.3.1 (Agilent Technologies).
Sample_data_processing	RMA normalization performed in GeneSpring v 7.3.1. Log2 RMA data was quantile transformed to median sample baseline.
Sample_platform_id	GPL1261
Sample_contact_name	Riccardo,,Natoli
Sample_contact_email	riccardo.natoli@anu.edu.au
Sample_contact_phone	+ 61 2 6125 8559
Sample_contact_fax	+ 61 2 6125 8680
Sample_contact_laboratory	Provis Lab
Sample_contact_department	CMBE
Sample_contact_institute	ANU
Sample_contact_address	Sullivans Creek Road
Sample_contact_city	Acton
Sample_contact_state	ACT
Sample_contact_zip/postal_code	2617
Sample_contact_country	Australia
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM575nnn/GSM575238/suppl/GSM575238.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM575nnn/GSM575238/suppl/GSM575238.CHP.gz
Sample_series_id	GSE23437
Sample_data_row_count	45101

GSM575239

GPL1261

Mouse retina_14 days_ rep1

retina, 14 days exposure to 75% hyperoxia

strain: C57BL/6J tissue: Retina age: Adult P80 - 120

Gene expression data from mouse retina exposued to 14 days 75% hyperoxia

Sample_geo_accession	GSM575239
Sample_status	Public on Aug 06 2010
Sample_submission_date	Aug 05 2010
Sample_last_update_date	Aug 05 2010
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Animals were placed in an oxygen chamber and exposed to 75% oxygen for a period of either 3, 7 or 14 days.
Sample_growth_protocol_ch1	Animals were raised in cyclic light conditions using 12hrs dim (5 lux) and 12hrs dark (no light).
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was extracted from each retinal biopsy using Trizol and chloroform to separate the RNA from other cell components. The RNA was then purified and isolated using RNAqueous-Micro RNA isolation kit (Ambion, Inc., Austin, TX). In some cases where genomic DNA contamination was a concern, the RNA was DNAse treated.
Sample_label_ch1	Biotin
Sample_label_protocol_ch1	Biotin-labeled cRNA was prepared according to the standard Affymetrix protocol from 500ng total RNA (Affymetrix manual, P/N 702646 Rev. 7).
Sample_hyb_protocol	Standard Affymetrix protocols were followed in the hybridisation, blocking and washing (GeneChip® Expression Analysis Technical Manual (P/N 702232 Rev. 3)).
Sample_scan_protocol	The arrays were scanned on the GCS 3000 Affymetrix high resolution scanner and analyzed using the GeneChip Operating Software v1.4 (GCOS; Affymetrix) and GeneSpring v 7.3.1 (Agilent Technologies).
Sample_data_processing	RMA normalization performed in GeneSpring v 7.3.1. Log2 RMA data was quantile transformed to median sample baseline.
Sample_platform_id	GPL1261
Sample_contact_name	Riccardo,,Natoli
Sample_contact_email	riccardo.natoli@anu.edu.au
Sample_contact_phone	+ 61 2 6125 8559
Sample_contact_fax	+ 61 2 6125 8680
Sample_contact_laboratory	Provis Lab
Sample_contact_department	CMBE
Sample_contact_institute	ANU
Sample_contact_address	Sullivans Creek Road
Sample_contact_city	Acton
Sample_contact_state	ACT
Sample_contact_zip/postal_code	2617
Sample_contact_country	Australia
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM575nnn/GSM575239/suppl/GSM575239.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM575nnn/GSM575239/suppl/GSM575239.CHP.gz
Sample_series_id	GSE23437
Sample_data_row_count	45101

GSM575240

GPL1261

Mouse retina_14 days_ rep2

retina, 14 days exposure to 75% hyperoxia

strain: C57BL/6J tissue: Retina age: Adult P80 - 120

Gene expression data from mouse retina exposued to 14 days 75% hyperoxia

Sample_geo_accession	GSM575240
Sample_status	Public on Aug 06 2010
Sample_submission_date	Aug 05 2010
Sample_last_update_date	Aug 05 2010
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Animals were placed in an oxygen chamber and exposed to 75% oxygen for a period of either 3, 7 or 14 days.
Sample_growth_protocol_ch1	Animals were raised in cyclic light conditions using 12hrs dim (5 lux) and 12hrs dark (no light).
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was extracted from each retinal biopsy using Trizol and chloroform to separate the RNA from other cell components. The RNA was then purified and isolated using RNAqueous-Micro RNA isolation kit (Ambion, Inc., Austin, TX). In some cases where genomic DNA contamination was a concern, the RNA was DNAse treated.
Sample_label_ch1	Biotin
Sample_label_protocol_ch1	Biotin-labeled cRNA was prepared according to the standard Affymetrix protocol from 500ng total RNA (Affymetrix manual, P/N 702646 Rev. 7).
Sample_hyb_protocol	Standard Affymetrix protocols were followed in the hybridisation, blocking and washing (GeneChip® Expression Analysis Technical Manual (P/N 702232 Rev. 3)).
Sample_scan_protocol	The arrays were scanned on the GCS 3000 Affymetrix high resolution scanner and analyzed using the GeneChip Operating Software v1.4 (GCOS; Affymetrix) and GeneSpring v 7.3.1 (Agilent Technologies).
Sample_data_processing	RMA normalization performed in GeneSpring v 7.3.1. Log2 RMA data was quantile transformed to median sample baseline.
Sample_platform_id	GPL1261
Sample_contact_name	Riccardo,,Natoli
Sample_contact_email	riccardo.natoli@anu.edu.au
Sample_contact_phone	+ 61 2 6125 8559
Sample_contact_fax	+ 61 2 6125 8680
Sample_contact_laboratory	Provis Lab
Sample_contact_department	CMBE
Sample_contact_institute	ANU
Sample_contact_address	Sullivans Creek Road
Sample_contact_city	Acton
Sample_contact_state	ACT
Sample_contact_zip/postal_code	2617
Sample_contact_country	Australia
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM575nnn/GSM575240/suppl/GSM575240.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM575nnn/GSM575240/suppl/GSM575240.CHP.gz
Sample_series_id	GSE23437
Sample_data_row_count	45101

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