Search results for the GEO ID: GSE23556  |
(Click on the check boxes provided under "Select for analysis", to initiate grouping) |
(Once the selection is made, click on "Add groups" in "Make groups for comparison", to make a group. Scroll down) |
|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM577851 | GPL1261 |
|
LSK control 1
|
LSK cells SRF wt 1
|
cell type: sorted LSK cells
strain: C57BL/6
genotype/variation: SRF wt
|
Gene expression data from LSK cells SRF wt
|
| Sample_geo_accession | GSM577851
| | Sample_status | Public on Aug 12 2010
| | Sample_submission_date | Aug 10 2010
| | Sample_last_update_date | Aug 23 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | FACS-sorted LSK (Lin-Sca1+c-Kit+) cells were isolated from pIpC-treated Srf flx/flx ;Mx1-Cre and Srf+/+;Mx1-Cre pooled animals
| | Sample_growth_protocol_ch1 | Srf flx/flx mice were crossed with the polyinosine-polycytidine (pIpC)-inducible Mx1-Cre recombinase mouse line. Induction was performed on 6 weeks-old mice by three intra-peritoneal (IP) injections of 200 µg/dose pIpC (Invivogen) every 2 days and mice were analyzed 35 days after the last injection.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNAs were collected from invalidated or not 105 LSKs (n=3 per genotype) using the RNeasy micro kit (Qiagen) according to manufacturer’s protocol
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 30 ng of total RNA were amplified and labeled using the NuGen Ovation RNA Amplification System V2 followed by the FL-Ovation cDNA Biotin Module V2 according to manufacturer’s protocol. (NuGEN Technologies Inc.).
| | Sample_hyb_protocol | 3,75 ug of cRNA were hybridized for 16 hr at 45°C on GeneChip Affymetrix MOE430A. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip 3000 7G Scanner
| | Sample_data_processing | The data were analyzed with GeneChip Operating Software 1.4
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Virginie,,PENARD
| | Sample_contact_email | virginie.penard-lacronique@inserm.fr
| | Sample_contact_phone | 33-1-42 11 42 33
| | Sample_contact_fax | 33-1-42 11 52 40
| | Sample_contact_department | Institut Gustave Roussy
| | Sample_contact_institute | INSERM U985
| | Sample_contact_address | 39 rue Camille Desmoulins
| | Sample_contact_city | Villejuif
| | Sample_contact_zip/postal_code | 94805
| | Sample_contact_country | France
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM577nnn/GSM577851/suppl/GSM577851.CEL.gz
| | Sample_series_id | GSE23556
| | Sample_data_row_count | 45101
| | |
|
GSM577852 | GPL1261 |
|
LSK control 2
|
LSK cells SRF wt 2
|
cell type: sorted LSK cells
strain: C57BL/6
genotype/variation: SRF wt
|
Gene expression data from LSK cells SRF wt
|
| Sample_geo_accession | GSM577852
| | Sample_status | Public on Aug 12 2010
| | Sample_submission_date | Aug 10 2010
| | Sample_last_update_date | Aug 23 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | FACS-sorted LSK (Lin-Sca1+c-Kit+) cells were isolated from pIpC-treated Srf flx/flx ;Mx1-Cre and Srf+/+;Mx1-Cre pooled animals
| | Sample_growth_protocol_ch1 | Srf flx/flx mice were crossed with the polyinosine-polycytidine (pIpC)-inducible Mx1-Cre recombinase mouse line. Induction was performed on 6 weeks-old mice by three intra-peritoneal (IP) injections of 200 µg/dose pIpC (Invivogen) every 2 days and mice were analyzed 35 days after the last injection.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNAs were collected from invalidated or not 105 LSKs (n=3 per genotype) using the RNeasy micro kit (Qiagen) according to manufacturer’s protocol
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 30 ng of total RNA were amplified and labeled using the NuGen Ovation RNA Amplification System V2 followed by the FL-Ovation cDNA Biotin Module V2 according to manufacturer’s protocol. (NuGEN Technologies Inc.).
| | Sample_hyb_protocol | 3,75 ug of cRNA were hybridized for 16 hr at 45°C on GeneChip Affymetrix MOE430A. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip 3000 7G Scanner
| | Sample_data_processing | The data were analyzed with GeneChip Operating Software 1.4
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Virginie,,PENARD
| | Sample_contact_email | virginie.penard-lacronique@inserm.fr
| | Sample_contact_phone | 33-1-42 11 42 33
| | Sample_contact_fax | 33-1-42 11 52 40
| | Sample_contact_department | Institut Gustave Roussy
| | Sample_contact_institute | INSERM U985
| | Sample_contact_address | 39 rue Camille Desmoulins
| | Sample_contact_city | Villejuif
| | Sample_contact_zip/postal_code | 94805
| | Sample_contact_country | France
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM577nnn/GSM577852/suppl/GSM577852.CEL.gz
| | Sample_series_id | GSE23556
| | Sample_data_row_count | 45101
| | |
|
GSM577853 | GPL1261 |
|
LSK control 3
|
LSK cells SRF wt 3
|
cell type: sorted LSK cells
strain: C57BL/6
genotype/variation: SRF wt
|
Gene expression data from LSK cells SRF wt
|
| Sample_geo_accession | GSM577853
| | Sample_status | Public on Aug 12 2010
| | Sample_submission_date | Aug 10 2010
| | Sample_last_update_date | Aug 23 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | FACS-sorted LSK (Lin-Sca1+c-Kit+) cells were isolated from pIpC-treated Srf flx/flx ;Mx1-Cre and Srf+/+;Mx1-Cre pooled animals
| | Sample_growth_protocol_ch1 | Srf flx/flx mice were crossed with the polyinosine-polycytidine (pIpC)-inducible Mx1-Cre recombinase mouse line. Induction was performed on 6 weeks-old mice by three intra-peritoneal (IP) injections of 200 µg/dose pIpC (Invivogen) every 2 days and mice were analyzed 35 days after the last injection.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNAs were collected from invalidated or not 105 LSKs (n=3 per genotype) using the RNeasy micro kit (Qiagen) according to manufacturer’s protocol
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 30 ng of total RNA were amplified and labeled using the NuGen Ovation RNA Amplification System V2 followed by the FL-Ovation cDNA Biotin Module V2 according to manufacturer’s protocol. (NuGEN Technologies Inc.).
| | Sample_hyb_protocol | 3,75 ug of cRNA were hybridized for 16 hr at 45°C on GeneChip Affymetrix MOE430A. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip 3000 7G Scanner
| | Sample_data_processing | The data were analyzed with GeneChip Operating Software 1.4
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Virginie,,PENARD
| | Sample_contact_email | virginie.penard-lacronique@inserm.fr
| | Sample_contact_phone | 33-1-42 11 42 33
| | Sample_contact_fax | 33-1-42 11 52 40
| | Sample_contact_department | Institut Gustave Roussy
| | Sample_contact_institute | INSERM U985
| | Sample_contact_address | 39 rue Camille Desmoulins
| | Sample_contact_city | Villejuif
| | Sample_contact_zip/postal_code | 94805
| | Sample_contact_country | France
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM577nnn/GSM577853/suppl/GSM577853.CEL.gz
| | Sample_series_id | GSE23556
| | Sample_data_row_count | 45101
| | |
|
GSM577854 | GPL1261 |
|
LSK SRF-null 1
|
LSK cells SRF KO 1
|
cell type: sorted LSK cells
strain: C57BL/6
genotype/variation: SRF KO
|
Gene expression data from LSK cells SRF KO
|
| Sample_geo_accession | GSM577854
| | Sample_status | Public on Aug 12 2010
| | Sample_submission_date | Aug 10 2010
| | Sample_last_update_date | Aug 23 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | FACS-sorted LSK (Lin-Sca1+c-Kit+) cells were isolated from pIpC-treated Srf flx/flx ;Mx1-Cre and Srf+/+;Mx1-Cre pooled animals
| | Sample_growth_protocol_ch1 | Srf flx/flx mice were crossed with the polyinosine-polycytidine (pIpC)-inducible Mx1-Cre recombinase mouse line. Induction was performed on 6 weeks-old mice by three intra-peritoneal (IP) injections of 200 µg/dose pIpC (Invivogen) every 2 days and mice were analyzed 35 days after the last injection.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNAs were collected from invalidated or not 105 LSKs (n=3 per genotype) using the RNeasy micro kit (Qiagen) according to manufacturer’s protocol
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 30 ng of total RNA were amplified and labeled using the NuGen Ovation RNA Amplification System V2 followed by the FL-Ovation cDNA Biotin Module V2 according to manufacturer’s protocol. (NuGEN Technologies Inc.).
| | Sample_hyb_protocol | 3,75 ug of cRNA were hybridized for 16 hr at 45°C on GeneChip Affymetrix MOE430A. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip 3000 7G Scanner
| | Sample_data_processing | The data were analyzed with GeneChip Operating Software 1.4
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Virginie,,PENARD
| | Sample_contact_email | virginie.penard-lacronique@inserm.fr
| | Sample_contact_phone | 33-1-42 11 42 33
| | Sample_contact_fax | 33-1-42 11 52 40
| | Sample_contact_department | Institut Gustave Roussy
| | Sample_contact_institute | INSERM U985
| | Sample_contact_address | 39 rue Camille Desmoulins
| | Sample_contact_city | Villejuif
| | Sample_contact_zip/postal_code | 94805
| | Sample_contact_country | France
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM577nnn/GSM577854/suppl/GSM577854.CEL.gz
| | Sample_series_id | GSE23556
| | Sample_data_row_count | 45101
| | |
|
GSM577855 | GPL1261 |
|
LSK SRF-null 2
|
LSK cells SRF KO 2
|
cell type: sorted LSK cells
strain: C57BL/6
genotype/variation: SRF KO
|
Gene expression data from LSK cells SRF KO
|
| Sample_geo_accession | GSM577855
| | Sample_status | Public on Aug 12 2010
| | Sample_submission_date | Aug 10 2010
| | Sample_last_update_date | Aug 23 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | FACS-sorted LSK (Lin-Sca1+c-Kit+) cells were isolated from pIpC-treated Srf flx/flx ;Mx1-Cre and Srf+/+;Mx1-Cre pooled animals
| | Sample_growth_protocol_ch1 | Srf flx/flx mice were crossed with the polyinosine-polycytidine (pIpC)-inducible Mx1-Cre recombinase mouse line. Induction was performed on 6 weeks-old mice by three intra-peritoneal (IP) injections of 200 µg/dose pIpC (Invivogen) every 2 days and mice were analyzed 35 days after the last injection.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNAs were collected from invalidated or not 105 LSKs (n=3 per genotype) using the RNeasy micro kit (Qiagen) according to manufacturer’s protocol
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 30 ng of total RNA were amplified and labeled using the NuGen Ovation RNA Amplification System V2 followed by the FL-Ovation cDNA Biotin Module V2 according to manufacturer’s protocol. (NuGEN Technologies Inc.).
| | Sample_hyb_protocol | 3,75 ug of cRNA were hybridized for 16 hr at 45°C on GeneChip Affymetrix MOE430A. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip 3000 7G Scanner
| | Sample_data_processing | The data were analyzed with GeneChip Operating Software 1.4
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Virginie,,PENARD
| | Sample_contact_email | virginie.penard-lacronique@inserm.fr
| | Sample_contact_phone | 33-1-42 11 42 33
| | Sample_contact_fax | 33-1-42 11 52 40
| | Sample_contact_department | Institut Gustave Roussy
| | Sample_contact_institute | INSERM U985
| | Sample_contact_address | 39 rue Camille Desmoulins
| | Sample_contact_city | Villejuif
| | Sample_contact_zip/postal_code | 94805
| | Sample_contact_country | France
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM577nnn/GSM577855/suppl/GSM577855.CEL.gz
| | Sample_series_id | GSE23556
| | Sample_data_row_count | 45101
| | |
|
GSM577856 | GPL1261 |
|
LSK SRF-null 3
|
LSK cells SRF KO 3
|
cell type: sorted LSK cells
strain: C57BL/6
genotype/variation: SRF KO
|
Gene expression data from LSK cells SRF KO
|
| Sample_geo_accession | GSM577856
| | Sample_status | Public on Aug 12 2010
| | Sample_submission_date | Aug 10 2010
| | Sample_last_update_date | Aug 23 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | FACS-sorted LSK (Lin-Sca1+c-Kit+) cells were isolated from pIpC-treated Srf flx/flx ;Mx1-Cre and Srf+/+;Mx1-Cre pooled animals
| | Sample_growth_protocol_ch1 | Srf flx/flx mice were crossed with the polyinosine-polycytidine (pIpC)-inducible Mx1-Cre recombinase mouse line. Induction was performed on 6 weeks-old mice by three intra-peritoneal (IP) injections of 200 µg/dose pIpC (Invivogen) every 2 days and mice were analyzed 35 days after the last injection.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNAs were collected from invalidated or not 105 LSKs (n=3 per genotype) using the RNeasy micro kit (Qiagen) according to manufacturer’s protocol
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 30 ng of total RNA were amplified and labeled using the NuGen Ovation RNA Amplification System V2 followed by the FL-Ovation cDNA Biotin Module V2 according to manufacturer’s protocol. (NuGEN Technologies Inc.).
| | Sample_hyb_protocol | 3,75 ug of cRNA were hybridized for 16 hr at 45°C on GeneChip Affymetrix MOE430A. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip 3000 7G Scanner
| | Sample_data_processing | The data were analyzed with GeneChip Operating Software 1.4
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Virginie,,PENARD
| | Sample_contact_email | virginie.penard-lacronique@inserm.fr
| | Sample_contact_phone | 33-1-42 11 42 33
| | Sample_contact_fax | 33-1-42 11 52 40
| | Sample_contact_department | Institut Gustave Roussy
| | Sample_contact_institute | INSERM U985
| | Sample_contact_address | 39 rue Camille Desmoulins
| | Sample_contact_city | Villejuif
| | Sample_contact_zip/postal_code | 94805
| | Sample_contact_country | France
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM577nnn/GSM577856/suppl/GSM577856.CEL.gz
| | Sample_series_id | GSE23556
| | Sample_data_row_count | 45101
| | |
|
| |
| |
Make groups for comparisons |
(2 groups will be compared at a time) |
|
| Select GSMs and click on "Add groups" |
Enter the group name here: |
|
|
|
Select expression type |
| Transcripts profile based on; |
A. Differential status (Up/Down regulation) |
|
|
Regulation type |
|
Fold change |
|
p-value |
|
|
|
B. Absolute calls (Transcribed/Not-detected) |
|
|
| Derive calls within/across groups |
Within groups |
|
|
Detection status |
|
Percentage detection |
|
|
Across groups |
|
|
Detection status |
First group: |
- |
Second group:
|
Percentage detection |
First group: |
- |
Second group:
|
|
|
| |
Filter results by number of probes |
|
|
