Search results for the GEO ID: GSE23570 |
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GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM578131 | GPL1261 |
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ATDC5_CHX+PBS
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ATDC5_CHX+PBS_30min
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cell line: chondrogenic cell line ATDC5
agent: CHX+PBS(control)
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control
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Sample_geo_accession | GSM578131
| Sample_status | Public on Sep 01 2010
| Sample_submission_date | Aug 11 2010
| Sample_last_update_date | Aug 13 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA from ATDC5 cells for microarray and real-time PCR evaluation was extracted using TRIzol reagent (Invitrogen, Carlsbad, CA). Following ethanol precipitation, total RNA was applied to an RNeasy column (Qiagen, Valencia, CA) for further purification and treated with DNase according to the manufacturer’s protocol.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Standard Affymetrix protocol
| Sample_hyb_protocol | Target preparation and hybridization to the Affymetrix Murine 430 2.0 GeneChip was carried out following the standard protocol (http://www.affymetrix.com).
| Sample_scan_protocol | Standard Affymetrix protocol.
| Sample_data_processing | The data obtained from the hybridization was preprocessed using Affymetrix GeneChip Command Console Software (AGCC) and Expression Console Software to generate probe set intensity data (Affymetrix, Santa Clara, CA).
| Sample_data_processing | MAS5.0 signal intensity data.
| Sample_platform_id | GPL1261
| Sample_contact_name | wei wei,,chen
| Sample_contact_email | chenww0116@hotmail.com
| Sample_contact_institute | UCLA
| Sample_contact_address | 10833 Le Conte Avenue
| Sample_contact_city | los angeles
| Sample_contact_state | CA
| Sample_contact_zip/postal_code | 90095
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM578nnn/GSM578131/suppl/GSM578131.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM578nnn/GSM578131/suppl/GSM578131.CHP.gz
| Sample_series_id | GSE23570
| Sample_data_row_count | 45101
| |
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GSM578133 | GPL1261 |
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ATDC5_CHX+Nell-1
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ATDC5_CHX+Nell-1_30min
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cell line: chondrogenic cell line ATDC5
agent: CHX+Nell-1
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treatment
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Sample_geo_accession | GSM578133
| Sample_status | Public on Sep 01 2010
| Sample_submission_date | Aug 11 2010
| Sample_last_update_date | Aug 13 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA from ATDC5 cells for microarray and real-time PCR evaluation was extracted using TRIzol reagent (Invitrogen, Carlsbad, CA). Following ethanol precipitation, total RNA was applied to an RNeasy column (Qiagen, Valencia, CA) for further purification and treated with DNase according to the manufacturer’s protocol.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Standard Affymetrix protocol
| Sample_hyb_protocol | Target preparation and hybridization to the Affymetrix Murine 430 2.0 GeneChip was carried out following the standard protocol (http://www.affymetrix.com).
| Sample_scan_protocol | Standard Affymetrix protocol.
| Sample_data_processing | The data obtained from the hybridization was preprocessed using Affymetrix GeneChip Command Console Software (AGCC) and Expression Console Software to generate probe set intensity data (Affymetrix, Santa Clara, CA).
| Sample_data_processing | MAS5.0 signal intensity data.
| Sample_platform_id | GPL1261
| Sample_contact_name | wei wei,,chen
| Sample_contact_email | chenww0116@hotmail.com
| Sample_contact_institute | UCLA
| Sample_contact_address | 10833 Le Conte Avenue
| Sample_contact_city | los angeles
| Sample_contact_state | CA
| Sample_contact_zip/postal_code | 90095
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM578nnn/GSM578133/suppl/GSM578133.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM578nnn/GSM578133/suppl/GSM578133.CHP.gz
| Sample_series_id | GSE23570
| Sample_data_row_count | 45101
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