Search results for the GEO ID: GSE23583  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM579884 | GPL570 |
|
dH1F_Fibroblast_1
|
fibroblast, untransfected
|
cell type: dH1F fibroblast
reprogramming protocol: n/a
|
Gene expression data obtained from fibroblasts
|
| Sample_geo_accession | GSM579884
| | Sample_status | Public on Sep 30 2010
| | Sample_submission_date | Aug 16 2010
| | Sample_last_update_date | Sep 27 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | Fibroblasts were grown in alpha-MEM containing 10 % inactivated fetal serum (IFS), 50 U/ml penicillin, 50 mg/ml streptomycin, and 1 mM L-glutamine. Replacement,10 ng/ml bFGF, 1 mM L-glutamine, 100 uM nonessential amino acids, 100 uM 2-mercaptoethanol, 50 U/ml penicillin, and 50 mg/ml streptomycin).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol isolation followed by Rneasy (Qiagen) column purification were performed according to manufacturer's specifications
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Preparation of biotinylated cDNA was performed according to the standard Nugen amplification sample preparation protocol, starting with 100ng of total RNA
| | Sample_hyb_protocol | cDNA was fragmented and 10ug hybridized to an Affymetrix U133 Plus 2.0 array for 16 hours. Arrays were washed and stained using the Affy 450 Fluidics Station according to the manufacturer's protocol
| | Sample_scan_protocol | Arrays were scanned using a GeneChip 3000 scanner according to the manufacturers recommendation
| | Sample_data_processing | RMA (Bioconductor Affy Package)
| | Sample_platform_id | GPL570
| | Sample_contact_name | Hu,,Li
| | Sample_contact_email | huli@bu.edu
| | Sample_contact_laboratory | James Collins
| | Sample_contact_institute | Wyss Institute
| | Sample_contact_address | 3 Blackfan Circle
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02115
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM579nnn/GSM579884/suppl/GSM579884.CEL.gz
| | Sample_series_id | GSE23583
| | Sample_data_row_count | 54613
| | |
|
GSM579885 | GPL570 |
|
dH1F_Fibroblast_2
|
fibroblast, untransfected
|
cell type: dH1F fibroblast
reprogramming protocol: n/a
|
Gene expression data obtained from fibroblasts
|
| Sample_geo_accession | GSM579885
| | Sample_status | Public on Sep 30 2010
| | Sample_submission_date | Aug 16 2010
| | Sample_last_update_date | Sep 27 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | Fibroblasts were grown in alpha-MEM containing 10 % inactivated fetal serum (IFS), 50 U/ml penicillin, 50 mg/ml streptomycin, and 1 mM L-glutamine. Replacement,10 ng/ml bFGF, 1 mM L-glutamine, 100 uM nonessential amino acids, 100 uM 2-mercaptoethanol, 50 U/ml penicillin, and 50 mg/ml streptomycin).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol isolation followed by Rneasy (Qiagen) column purification were performed according to manufacturer's specifications
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Preparation of biotinylated cDNA was performed according to the standard Nugen amplification sample preparation protocol, starting with 100ng of total RNA
| | Sample_hyb_protocol | cDNA was fragmented and 10ug hybridized to an Affymetrix U133 Plus 2.0 array for 16 hours. Arrays were washed and stained using the Affy 450 Fluidics Station according to the manufacturer's protocol
| | Sample_scan_protocol | Arrays were scanned using a GeneChip 3000 scanner according to the manufacturers recommendation
| | Sample_data_processing | RMA (Bioconductor Affy Package)
| | Sample_platform_id | GPL570
| | Sample_contact_name | Hu,,Li
| | Sample_contact_email | huli@bu.edu
| | Sample_contact_laboratory | James Collins
| | Sample_contact_institute | Wyss Institute
| | Sample_contact_address | 3 Blackfan Circle
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02115
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM579nnn/GSM579885/suppl/GSM579885.CEL.gz
| | Sample_series_id | GSE23583
| | Sample_data_row_count | 54613
| | |
|
GSM579886 | GPL570 |
|
BJ_Fibroblast_1
|
fibroblast, untransfected
|
cell type: BJ fibroblast
reprogramming protocol: n/a
|
Gene expression data obtained from fibroblasts
|
| Sample_geo_accession | GSM579886
| | Sample_status | Public on Sep 30 2010
| | Sample_submission_date | Aug 16 2010
| | Sample_last_update_date | Sep 27 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | Fibroblasts were grown in alpha-MEM containing 10 % inactivated fetal serum (IFS), 50 U/ml penicillin, 50 mg/ml streptomycin, and 1 mM L-glutamine. Replacement,10 ng/ml bFGF, 1 mM L-glutamine, 100 uM nonessential amino acids, 100 uM 2-mercaptoethanol, 50 U/ml penicillin, and 50 mg/ml streptomycin).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol isolation followed by Rneasy (Qiagen) column purification were performed according to manufacturer's specifications
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Preparation of biotinylated cDNA was performed according to the standard Nugen amplification sample preparation protocol, starting with 100ng of total RNA
| | Sample_hyb_protocol | cDNA was fragmented and 10ug hybridized to an Affymetrix U133 Plus 2.0 array for 16 hours. Arrays were washed and stained using the Affy 450 Fluidics Station according to the manufacturer's protocol
| | Sample_scan_protocol | Arrays were scanned using a GeneChip 3000 scanner according to the manufacturers recommendation
| | Sample_data_processing | RMA (Bioconductor Affy Package)
| | Sample_platform_id | GPL570
| | Sample_contact_name | Hu,,Li
| | Sample_contact_email | huli@bu.edu
| | Sample_contact_laboratory | James Collins
| | Sample_contact_institute | Wyss Institute
| | Sample_contact_address | 3 Blackfan Circle
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02115
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM579nnn/GSM579886/suppl/GSM579886.CEL.gz
| | Sample_series_id | GSE23583
| | Sample_data_row_count | 54613
| | |
|
GSM579887 | GPL570 |
|
BJ_Fibroblast_2
|
fibroblast, untransfected
|
cell type: BJ fibroblast
reprogramming protocol: n/a
|
Gene expression data obtained from fibroblasts
|
| Sample_geo_accession | GSM579887
| | Sample_status | Public on Sep 30 2010
| | Sample_submission_date | Aug 16 2010
| | Sample_last_update_date | Sep 27 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | Fibroblasts were grown in alpha-MEM containing 10 % inactivated fetal serum (IFS), 50 U/ml penicillin, 50 mg/ml streptomycin, and 1 mM L-glutamine. Replacement,10 ng/ml bFGF, 1 mM L-glutamine, 100 uM nonessential amino acids, 100 uM 2-mercaptoethanol, 50 U/ml penicillin, and 50 mg/ml streptomycin).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol isolation followed by Rneasy (Qiagen) column purification were performed according to manufacturer's specifications
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Preparation of biotinylated cDNA was performed according to the standard Nugen amplification sample preparation protocol, starting with 100ng of total RNA
| | Sample_hyb_protocol | cDNA was fragmented and 10ug hybridized to an Affymetrix U133 Plus 2.0 array for 16 hours. Arrays were washed and stained using the Affy 450 Fluidics Station according to the manufacturer's protocol
| | Sample_scan_protocol | Arrays were scanned using a GeneChip 3000 scanner according to the manufacturers recommendation
| | Sample_data_processing | RMA (Bioconductor Affy Package)
| | Sample_platform_id | GPL570
| | Sample_contact_name | Hu,,Li
| | Sample_contact_email | huli@bu.edu
| | Sample_contact_laboratory | James Collins
| | Sample_contact_institute | Wyss Institute
| | Sample_contact_address | 3 Blackfan Circle
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02115
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM579nnn/GSM579887/suppl/GSM579887.CEL.gz
| | Sample_series_id | GSE23583
| | Sample_data_row_count | 54613
| | |
|
GSM579888 | GPL570 |
|
MRC5_Fibroblast_1
|
fibroblast, untransfected
|
cell type: MRC5 fibroblast
reprogramming protocol: n/a
|
Gene expression data obtained from fibroblasts
|
| Sample_geo_accession | GSM579888
| | Sample_status | Public on Sep 30 2010
| | Sample_submission_date | Aug 16 2010
| | Sample_last_update_date | Sep 27 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | Fibroblasts were grown in alpha-MEM containing 10 % inactivated fetal serum (IFS), 50 U/ml penicillin, 50 mg/ml streptomycin, and 1 mM L-glutamine. Replacement,10 ng/ml bFGF, 1 mM L-glutamine, 100 uM nonessential amino acids, 100 uM 2-mercaptoethanol, 50 U/ml penicillin, and 50 mg/ml streptomycin).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol isolation followed by Rneasy (Qiagen) column purification were performed according to manufacturer's specifications
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Preparation of biotinylated cDNA was performed according to the standard Nugen amplification sample preparation protocol, starting with 100ng of total RNA
| | Sample_hyb_protocol | cDNA was fragmented and 10ug hybridized to an Affymetrix U133 Plus 2.0 array for 16 hours. Arrays were washed and stained using the Affy 450 Fluidics Station according to the manufacturer's protocol
| | Sample_scan_protocol | Arrays were scanned using a GeneChip 3000 scanner according to the manufacturers recommendation
| | Sample_data_processing | RMA (Bioconductor Affy Package)
| | Sample_platform_id | GPL570
| | Sample_contact_name | Hu,,Li
| | Sample_contact_email | huli@bu.edu
| | Sample_contact_laboratory | James Collins
| | Sample_contact_institute | Wyss Institute
| | Sample_contact_address | 3 Blackfan Circle
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02115
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM579nnn/GSM579888/suppl/GSM579888.CEL.gz
| | Sample_series_id | GSE23583
| | Sample_data_row_count | 54613
| | |
|
GSM579889 | GPL570 |
|
MRC5_Fibroblast_2
|
fibroblast, untransfected
|
cell type: MRC5 fibroblast
reprogramming protocol: n/a
|
Gene expression data obtained from fibroblasts
|
| Sample_geo_accession | GSM579889
| | Sample_status | Public on Sep 30 2010
| | Sample_submission_date | Aug 16 2010
| | Sample_last_update_date | Sep 27 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | Fibroblasts were grown in alpha-MEM containing 10 % inactivated fetal serum (IFS), 50 U/ml penicillin, 50 mg/ml streptomycin, and 1 mM L-glutamine. Replacement,10 ng/ml bFGF, 1 mM L-glutamine, 100 uM nonessential amino acids, 100 uM 2-mercaptoethanol, 50 U/ml penicillin, and 50 mg/ml streptomycin).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol isolation followed by Rneasy (Qiagen) column purification were performed according to manufacturer's specifications
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Preparation of biotinylated cDNA was performed according to the standard Nugen amplification sample preparation protocol, starting with 100ng of total RNA
| | Sample_hyb_protocol | cDNA was fragmented and 10ug hybridized to an Affymetrix U133 Plus 2.0 array for 16 hours. Arrays were washed and stained using the Affy 450 Fluidics Station according to the manufacturer's protocol
| | Sample_scan_protocol | Arrays were scanned using a GeneChip 3000 scanner according to the manufacturers recommendation
| | Sample_data_processing | RMA (Bioconductor Affy Package)
| | Sample_platform_id | GPL570
| | Sample_contact_name | Hu,,Li
| | Sample_contact_email | huli@bu.edu
| | Sample_contact_laboratory | James Collins
| | Sample_contact_institute | Wyss Institute
| | Sample_contact_address | 3 Blackfan Circle
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02115
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM579nnn/GSM579889/suppl/GSM579889.CEL.gz
| | Sample_series_id | GSE23583
| | Sample_data_row_count | 54613
| | |
|
GSM579890 | GPL570 |
|
BJ_iPS_IH
|
viral derived iPS
|
cell type: iPS cells derived from BJ fibroblast
reprogramming protocol: viral derived iPS
|
Gene expression data obtained from BJ iPS
|
| Sample_geo_accession | GSM579890
| | Sample_status | Public on Sep 30 2010
| | Sample_submission_date | Aug 16 2010
| | Sample_last_update_date | Sep 27 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | For feeder-free culture of iPSCs, the plate was coated with 0.3mg/ml Matrigel (BD-Bioscience, San Jose, CA) and mTeSR (StemCell Technologies, Vancouver, Canada) was used according to the manufacturer’s instruction.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol isolation followed by Rneasy (Qiagen) column purification were performed according to manufacturer's specifications
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Preparation of biotinylated cDNA was performed according to the standard Nugen amplification sample preparation protocol, starting with 100ng of total RNA
| | Sample_hyb_protocol | cDNA was fragmented and 10ug hybridized to an Affymetrix U133 Plus 2.0 array for 16 hours. Arrays were washed and stained using the Affy 450 Fluidics Station according to the manufacturer's protocol
| | Sample_scan_protocol | Arrays were scanned using a GeneChip 3000 scanner according to the manufacturers recommendation
| | Sample_data_processing | RMA (Bioconductor Affy Package)
| | Sample_platform_id | GPL570
| | Sample_contact_name | Hu,,Li
| | Sample_contact_email | huli@bu.edu
| | Sample_contact_laboratory | James Collins
| | Sample_contact_institute | Wyss Institute
| | Sample_contact_address | 3 Blackfan Circle
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02115
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM579nnn/GSM579890/suppl/GSM579890.CEL.gz
| | Sample_series_id | GSE23583
| | Sample_data_row_count | 54613
| | |
|
GSM579891 | GPL570 |
|
dH1F_iPS3_IH3
|
viral derived iPS
|
cell type: iPS cells derived from dH1F fibroblast
reprogramming protocol: viral derived iPS
|
Gene expression data obtained from dH1F iPS
|
| Sample_geo_accession | GSM579891
| | Sample_status | Public on Sep 30 2010
| | Sample_submission_date | Aug 16 2010
| | Sample_last_update_date | Sep 27 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | For feeder-free culture of iPSCs, the plate was coated with 0.3mg/ml Matrigel (BD-Bioscience, San Jose, CA) and mTeSR (StemCell Technologies, Vancouver, Canada) was used according to the manufacturer’s instruction.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol isolation followed by Rneasy (Qiagen) column purification were performed according to manufacturer's specifications
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Preparation of biotinylated cDNA was performed according to the standard Nugen amplification sample preparation protocol, starting with 100ng of total RNA
| | Sample_hyb_protocol | cDNA was fragmented and 10ug hybridized to an Affymetrix U133 Plus 2.0 array for 16 hours. Arrays were washed and stained using the Affy 450 Fluidics Station according to the manufacturer's protocol
| | Sample_scan_protocol | Arrays were scanned using a GeneChip 3000 scanner according to the manufacturers recommendation
| | Sample_data_processing | RMA (Bioconductor Affy Package)
| | Sample_platform_id | GPL570
| | Sample_contact_name | Hu,,Li
| | Sample_contact_email | huli@bu.edu
| | Sample_contact_laboratory | James Collins
| | Sample_contact_institute | Wyss Institute
| | Sample_contact_address | 3 Blackfan Circle
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02115
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM579nnn/GSM579891/suppl/GSM579891.CEL.gz
| | Sample_series_id | GSE23583
| | Sample_data_row_count | 54613
| | |
|
GSM579892 | GPL570 |
|
dH1CF16_iPS5_IH10
|
viral derived iPS
|
cell type: iPS cells derived from dH1CF fibroblast
reprogramming protocol: viral derived iPS
|
Gene expression data obtained from dH1F iPS
|
| Sample_geo_accession | GSM579892
| | Sample_status | Public on Sep 30 2010
| | Sample_submission_date | Aug 16 2010
| | Sample_last_update_date | Sep 27 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | For feeder-free culture of iPSCs, the plate was coated with 0.3mg/ml Matrigel (BD-Bioscience, San Jose, CA) and mTeSR (StemCell Technologies, Vancouver, Canada) was used according to the manufacturer’s instruction.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol isolation followed by Rneasy (Qiagen) column purification were performed according to manufacturer's specifications
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Preparation of biotinylated cDNA was performed according to the standard Nugen amplification sample preparation protocol, starting with 100ng of total RNA
| | Sample_hyb_protocol | cDNA was fragmented and 10ug hybridized to an Affymetrix U133 Plus 2.0 array for 16 hours. Arrays were washed and stained using the Affy 450 Fluidics Station according to the manufacturer's protocol
| | Sample_scan_protocol | Arrays were scanned using a GeneChip 3000 scanner according to the manufacturers recommendation
| | Sample_data_processing | RMA (Bioconductor Affy Package)
| | Sample_platform_id | GPL570
| | Sample_contact_name | Hu,,Li
| | Sample_contact_email | huli@bu.edu
| | Sample_contact_laboratory | James Collins
| | Sample_contact_institute | Wyss Institute
| | Sample_contact_address | 3 Blackfan Circle
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02115
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM579nnn/GSM579892/suppl/GSM579892.CEL.gz
| | Sample_series_id | GSE23583
| | Sample_data_row_count | 54613
| | |
|
GSM579893 | GPL570 |
|
dH1CF16_iPS5_IH20
|
viral derived iPS
|
cell type: iPS cells derived from dH1CF fibroblast
reprogramming protocol: viral derived iPS
|
Gene expression data obtained from dH1F iPS
|
| Sample_geo_accession | GSM579893
| | Sample_status | Public on Sep 30 2010
| | Sample_submission_date | Aug 16 2010
| | Sample_last_update_date | Sep 27 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | For feeder-free culture of iPSCs, the plate was coated with 0.3mg/ml Matrigel (BD-Bioscience, San Jose, CA) and mTeSR (StemCell Technologies, Vancouver, Canada) was used according to the manufacturer’s instruction.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol isolation followed by Rneasy (Qiagen) column purification were performed according to manufacturer's specifications
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Preparation of biotinylated cDNA was performed according to the standard Nugen amplification sample preparation protocol, starting with 100ng of total RNA
| | Sample_hyb_protocol | cDNA was fragmented and 10ug hybridized to an Affymetrix U133 Plus 2.0 array for 16 hours. Arrays were washed and stained using the Affy 450 Fluidics Station according to the manufacturer's protocol
| | Sample_scan_protocol | Arrays were scanned using a GeneChip 3000 scanner according to the manufacturers recommendation
| | Sample_data_processing | RMA (Bioconductor Affy Package)
| | Sample_platform_id | GPL570
| | Sample_contact_name | Hu,,Li
| | Sample_contact_email | huli@bu.edu
| | Sample_contact_laboratory | James Collins
| | Sample_contact_institute | Wyss Institute
| | Sample_contact_address | 3 Blackfan Circle
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02115
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM579nnn/GSM579893/suppl/GSM579893.CEL.gz
| | Sample_series_id | GSE23583
| | Sample_data_row_count | 54613
| | |
|
GSM579894 | GPL570 |
|
dH1CF16_iPS5_IH30
|
viral derived iPS
|
cell type: iPS cells derived from dH1CF fibroblast
reprogramming protocol: viral derived iPS
|
Gene expression data obtained from dH1F iPS
|
| Sample_geo_accession | GSM579894
| | Sample_status | Public on Sep 30 2010
| | Sample_submission_date | Aug 16 2010
| | Sample_last_update_date | Sep 27 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | For feeder-free culture of iPSCs, the plate was coated with 0.3mg/ml Matrigel (BD-Bioscience, San Jose, CA) and mTeSR (StemCell Technologies, Vancouver, Canada) was used according to the manufacturer’s instruction.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol isolation followed by Rneasy (Qiagen) column purification were performed according to manufacturer's specifications
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Preparation of biotinylated cDNA was performed according to the standard Nugen amplification sample preparation protocol, starting with 100ng of total RNA
| | Sample_hyb_protocol | cDNA was fragmented and 10ug hybridized to an Affymetrix U133 Plus 2.0 array for 16 hours. Arrays were washed and stained using the Affy 450 Fluidics Station according to the manufacturer's protocol
| | Sample_scan_protocol | Arrays were scanned using a GeneChip 3000 scanner according to the manufacturers recommendation
| | Sample_data_processing | RMA (Bioconductor Affy Package)
| | Sample_platform_id | GPL570
| | Sample_contact_name | Hu,,Li
| | Sample_contact_email | huli@bu.edu
| | Sample_contact_laboratory | James Collins
| | Sample_contact_institute | Wyss Institute
| | Sample_contact_address | 3 Blackfan Circle
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02115
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM579nnn/GSM579894/suppl/GSM579894.CEL.gz
| | Sample_series_id | GSE23583
| | Sample_data_row_count | 54613
| | |
|
GSM579895 | GPL570 |
|
dH1CF16_iPS5_IH32
|
viral derived iPS
|
cell type: iPS cells derived from dH1CF fibroblast
reprogramming protocol: viral derived iPS
|
Gene expression data obtained from dH1F iPS
|
| Sample_geo_accession | GSM579895
| | Sample_status | Public on Sep 30 2010
| | Sample_submission_date | Aug 16 2010
| | Sample_last_update_date | Sep 27 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | For feeder-free culture of iPSCs, the plate was coated with 0.3mg/ml Matrigel (BD-Bioscience, San Jose, CA) and mTeSR (StemCell Technologies, Vancouver, Canada) was used according to the manufacturer’s instruction.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol isolation followed by Rneasy (Qiagen) column purification were performed according to manufacturer's specifications
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Preparation of biotinylated cDNA was performed according to the standard Nugen amplification sample preparation protocol, starting with 100ng of total RNA
| | Sample_hyb_protocol | cDNA was fragmented and 10ug hybridized to an Affymetrix U133 Plus 2.0 array for 16 hours. Arrays were washed and stained using the Affy 450 Fluidics Station according to the manufacturer's protocol
| | Sample_scan_protocol | Arrays were scanned using a GeneChip 3000 scanner according to the manufacturers recommendation
| | Sample_data_processing | RMA (Bioconductor Affy Package)
| | Sample_platform_id | GPL570
| | Sample_contact_name | Hu,,Li
| | Sample_contact_email | huli@bu.edu
| | Sample_contact_laboratory | James Collins
| | Sample_contact_institute | Wyss Institute
| | Sample_contact_address | 3 Blackfan Circle
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02115
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM579nnn/GSM579895/suppl/GSM579895.CEL.gz
| | Sample_series_id | GSE23583
| | Sample_data_row_count | 54613
| | |
|
GSM579896 | GPL570 |
|
MRC5_iPS2_IH
|
viral derived iPS
|
cell type: iPS cells derived from MRC5 fibroblast
reprogramming protocol: viral derived iPS
|
Gene expression data obtained from MRC5 iPS
|
| Sample_geo_accession | GSM579896
| | Sample_status | Public on Sep 30 2010
| | Sample_submission_date | Aug 16 2010
| | Sample_last_update_date | Jan 25 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | For feeder-free culture of iPSCs, the plate was coated with 0.3mg/ml Matrigel (BD-Bioscience, San Jose, CA) and mTeSR (StemCell Technologies, Vancouver, Canada) was used according to the manufacturer’s instruction.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol isolation followed by Rneasy (Qiagen) column purification were performed according to manufacturer's specifications
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Preparation of biotinylated cDNA was performed according to the standard Nugen amplification sample preparation protocol, starting with 100ng of total RNA
| | Sample_hyb_protocol | cDNA was fragmented and 10ug hybridized to an Affymetrix U133 Plus 2.0 array for 16 hours. Arrays were washed and stained using the Affy 450 Fluidics Station according to the manufacturer's protocol
| | Sample_scan_protocol | Arrays were scanned using a GeneChip 3000 scanner according to the manufacturers recommendation
| | Sample_data_processing | RMA (Bioconductor Affy Package)
| | Sample_platform_id | GPL570
| | Sample_contact_name | Hu,,Li
| | Sample_contact_email | huli@bu.edu
| | Sample_contact_laboratory | James Collins
| | Sample_contact_institute | Wyss Institute
| | Sample_contact_address | 3 Blackfan Circle
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02115
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM579nnn/GSM579896/suppl/GSM579896.CEL.gz
| | Sample_series_id | GSE23583
| | Sample_data_row_count | 54613
| | |
|
GSM579897 | GPL570 |
|
MRC5_iPS2_2_IH
|
viral derived iPS
|
cell type: iPS cells derived from MRC5 fibroblast
reprogramming protocol: viral derived iPS
|
Gene expression data obtained from MRC5 iPS
|
| Sample_geo_accession | GSM579897
| | Sample_status | Public on Sep 30 2010
| | Sample_submission_date | Aug 16 2010
| | Sample_last_update_date | Sep 27 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | For feeder-free culture of iPSCs, the plate was coated with 0.3mg/ml Matrigel (BD-Bioscience, San Jose, CA) and mTeSR (StemCell Technologies, Vancouver, Canada) was used according to the manufacturer’s instruction.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol isolation followed by Rneasy (Qiagen) column purification were performed according to manufacturer's specifications
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Preparation of biotinylated cDNA was performed according to the standard Nugen amplification sample preparation protocol, starting with 100ng of total RNA
| | Sample_hyb_protocol | cDNA was fragmented and 10ug hybridized to an Affymetrix U133 Plus 2.0 array for 16 hours. Arrays were washed and stained using the Affy 450 Fluidics Station according to the manufacturer's protocol
| | Sample_scan_protocol | Arrays were scanned using a GeneChip 3000 scanner according to the manufacturers recommendation
| | Sample_data_processing | RMA (Bioconductor Affy Package)
| | Sample_platform_id | GPL570
| | Sample_contact_name | Hu,,Li
| | Sample_contact_email | huli@bu.edu
| | Sample_contact_laboratory | James Collins
| | Sample_contact_institute | Wyss Institute
| | Sample_contact_address | 3 Blackfan Circle
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02115
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM579nnn/GSM579897/suppl/GSM579897.CEL.gz
| | Sample_series_id | GSE23583
| | Sample_data_row_count | 54613
| | |
|
GSM579898 | GPL570 |
|
H1_ES-1
|
human ES
|
cell line: H1 Human embryonic stem cells
reprogramming protocol: n/a
|
Gene expression data obtained from human ES
|
| Sample_geo_accession | GSM579898
| | Sample_status | Public on Sep 30 2010
| | Sample_submission_date | Aug 16 2010
| | Sample_last_update_date | Sep 27 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | standard HESC growth conditions as described in Pick et al. (2009).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol isolation followed by Rneasy (Qiagen) column purification were performed according to manufacturer's specifications
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Preparation of biotinylated cDNA was performed according to the standard Nugen amplification sample preparation protocol, starting with 100ng of total RNA
| | Sample_hyb_protocol | cDNA was fragmented and 10ug hybridized to an Affymetrix U133 Plus 2.0 array for 16 hours. Arrays were washed and stained using the Affy 450 Fluidics Station according to the manufacturer's protocol
| | Sample_scan_protocol | Arrays were scanned using a GeneChip 3000 scanner according to the manufacturers recommendation
| | Sample_data_processing | RMA (Bioconductor Affy Package)
| | Sample_platform_id | GPL570
| | Sample_contact_name | Hu,,Li
| | Sample_contact_email | huli@bu.edu
| | Sample_contact_laboratory | James Collins
| | Sample_contact_institute | Wyss Institute
| | Sample_contact_address | 3 Blackfan Circle
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02115
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM579nnn/GSM579898/suppl/GSM579898.CEL.gz
| | Sample_relation | Reanalysis of: GSM551200
| | Sample_series_id | GSE23583
| | Sample_data_row_count | 54613
| | |
|
GSM579899 | GPL570 |
|
H1_ES-2
|
human ES
|
cell line: H1 Human embryonic stem cells
reprogramming protocol: n/a
|
Gene expression data obtained from human ES
|
| Sample_geo_accession | GSM579899
| | Sample_status | Public on Sep 30 2010
| | Sample_submission_date | Aug 16 2010
| | Sample_last_update_date | Sep 27 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | standard HESC growth conditions as described in Pick et al. (2009).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol isolation followed by Rneasy (Qiagen) column purification were performed according to manufacturer's specifications
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Preparation of biotinylated cDNA was performed according to the standard Nugen amplification sample preparation protocol, starting with 100ng of total RNA
| | Sample_hyb_protocol | cDNA was fragmented and 10ug hybridized to an Affymetrix U133 Plus 2.0 array for 16 hours. Arrays were washed and stained using the Affy 450 Fluidics Station according to the manufacturer's protocol
| | Sample_scan_protocol | Arrays were scanned using a GeneChip 3000 scanner according to the manufacturers recommendation
| | Sample_data_processing | RMA (Bioconductor Affy Package)
| | Sample_platform_id | GPL570
| | Sample_contact_name | Hu,,Li
| | Sample_contact_email | huli@bu.edu
| | Sample_contact_laboratory | James Collins
| | Sample_contact_institute | Wyss Institute
| | Sample_contact_address | 3 Blackfan Circle
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02115
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM579nnn/GSM579899/suppl/GSM579899.CEL.gz
| | Sample_relation | Reanalysis of: GSM551201
| | Sample_series_id | GSE23583
| | Sample_data_row_count | 54613
| | |
|
GSM579900 | GPL570 |
|
H1_OGNES_IH
|
human ES
|
cell line: H1 Human OGNES embryonic stem cells
reprogramming protocol: n/a
|
Gene expression data obtained from human ES
|
| Sample_geo_accession | GSM579900
| | Sample_status | Public on Sep 30 2010
| | Sample_submission_date | Aug 16 2010
| | Sample_last_update_date | Sep 27 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | standard HESC growth conditions as described in Pick et al. (2009).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol isolation followed by Rneasy (Qiagen) column purification were performed according to manufacturer's specifications
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Preparation of biotinylated cDNA was performed according to the standard Nugen amplification sample preparation protocol, starting with 100ng of total RNA
| | Sample_hyb_protocol | cDNA was fragmented and 10ug hybridized to an Affymetrix U133 Plus 2.0 array for 16 hours. Arrays were washed and stained using the Affy 450 Fluidics Station according to the manufacturer's protocol
| | Sample_scan_protocol | Arrays were scanned using a GeneChip 3000 scanner according to the manufacturers recommendation
| | Sample_data_processing | RMA (Bioconductor Affy Package)
| | Sample_platform_id | GPL570
| | Sample_contact_name | Hu,,Li
| | Sample_contact_email | huli@bu.edu
| | Sample_contact_laboratory | James Collins
| | Sample_contact_institute | Wyss Institute
| | Sample_contact_address | 3 Blackfan Circle
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02115
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM579nnn/GSM579900/suppl/GSM579900.CEL.gz
| | Sample_series_id | GSE23583
| | Sample_data_row_count | 54613
| | |
|
GSM579901 | GPL570 |
|
H9_ES-1
|
human ES
|
cell line: H9 Human embryonic stem cells
reprogramming protocol: n/a
|
Gene expression data obtained from human ES
|
| Sample_geo_accession | GSM579901
| | Sample_status | Public on Sep 30 2010
| | Sample_submission_date | Aug 16 2010
| | Sample_last_update_date | Sep 27 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | standard HESC growth conditions as described in Pick et al. (2009).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol isolation followed by Rneasy (Qiagen) column purification were performed according to manufacturer's specifications
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Preparation of biotinylated cDNA was performed according to the standard Nugen amplification sample preparation protocol, starting with 100ng of total RNA
| | Sample_hyb_protocol | cDNA was fragmented and 10ug hybridized to an Affymetrix U133 Plus 2.0 array for 16 hours. Arrays were washed and stained using the Affy 450 Fluidics Station according to the manufacturer's protocol
| | Sample_scan_protocol | Arrays were scanned using a GeneChip 3000 scanner according to the manufacturers recommendation
| | Sample_data_processing | RMA (Bioconductor Affy Package)
| | Sample_platform_id | GPL570
| | Sample_contact_name | Hu,,Li
| | Sample_contact_email | huli@bu.edu
| | Sample_contact_laboratory | James Collins
| | Sample_contact_institute | Wyss Institute
| | Sample_contact_address | 3 Blackfan Circle
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02115
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM579nnn/GSM579901/suppl/GSM579901.CEL.gz
| | Sample_relation | Reanalysis of: GSM551202
| | Sample_series_id | GSE23583
| | Sample_data_row_count | 54613
| | |
|
GSM579902 | GPL570 |
|
H9_ES-2
|
human ES
|
cell line: H9 Human embryonic stem cells
reprogramming protocol: n/a
|
Gene expression data obtained from human ES
|
| Sample_geo_accession | GSM579902
| | Sample_status | Public on Sep 30 2010
| | Sample_submission_date | Aug 16 2010
| | Sample_last_update_date | Sep 27 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | standard HESC growth conditions as described in Pick et al. (2009).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol isolation followed by Rneasy (Qiagen) column purification were performed according to manufacturer's specifications
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Preparation of biotinylated cDNA was performed according to the standard Nugen amplification sample preparation protocol, starting with 100ng of total RNA
| | Sample_hyb_protocol | cDNA was fragmented and 10ug hybridized to an Affymetrix U133 Plus 2.0 array for 16 hours. Arrays were washed and stained using the Affy 450 Fluidics Station according to the manufacturer's protocol
| | Sample_scan_protocol | Arrays were scanned using a GeneChip 3000 scanner according to the manufacturers recommendation
| | Sample_data_processing | RMA (Bioconductor Affy Package)
| | Sample_platform_id | GPL570
| | Sample_contact_name | Hu,,Li
| | Sample_contact_email | huli@bu.edu
| | Sample_contact_laboratory | James Collins
| | Sample_contact_institute | Wyss Institute
| | Sample_contact_address | 3 Blackfan Circle
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02115
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM579nnn/GSM579902/suppl/GSM579902.CEL.gz
| | Sample_relation | Reanalysis of: GSM551203
| | Sample_series_id | GSE23583
| | Sample_data_row_count | 54613
| | |
|
GSM579903 | GPL570 |
|
dH1F_RiPS_1.2
|
RNA iPS
|
cell type: iPS cells derived from dH1F fibroblast
reprogramming protocol: RNA derived iPS
|
Gene expression data obtained from dH1F RNA iPS
|
| Sample_geo_accession | GSM579903
| | Sample_status | Public on Sep 30 2010
| | Sample_submission_date | Aug 16 2010
| | Sample_last_update_date | Sep 27 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | Refer to online method of publication
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol isolation followed by Rneasy (Qiagen) column purification were performed according to manufacturer's specifications
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Preparation of biotinylated cDNA was performed according to the standard Nugen amplification sample preparation protocol, starting with 100ng of total RNA
| | Sample_hyb_protocol | cDNA was fragmented and 10ug hybridized to an Affymetrix U133 Plus 2.0 array for 16 hours. Arrays were washed and stained using the Affy 450 Fluidics Station according to the manufacturer's protocol
| | Sample_scan_protocol | Arrays were scanned using a GeneChip 3000 scanner according to the manufacturers recommendation
| | Sample_data_processing | RMA (Bioconductor Affy Package)
| | Sample_platform_id | GPL570
| | Sample_contact_name | Hu,,Li
| | Sample_contact_email | huli@bu.edu
| | Sample_contact_laboratory | James Collins
| | Sample_contact_institute | Wyss Institute
| | Sample_contact_address | 3 Blackfan Circle
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02115
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM579nnn/GSM579903/suppl/GSM579903.CEL.gz
| | Sample_series_id | GSE23583
| | Sample_data_row_count | 54613
| | |
|
GSM579904 | GPL570 |
|
dH1F_RiPS_1.3
|
RNA iPS
|
cell type: iPS cells derived from dH1F fibroblast
reprogramming protocol: RNA derived iPS
|
Gene expression data obtained from dH1F RNA iPS
|
| Sample_geo_accession | GSM579904
| | Sample_status | Public on Sep 30 2010
| | Sample_submission_date | Aug 16 2010
| | Sample_last_update_date | Sep 27 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | Refer to online method of publication
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol isolation followed by Rneasy (Qiagen) column purification were performed according to manufacturer's specifications
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Preparation of biotinylated cDNA was performed according to the standard Nugen amplification sample preparation protocol, starting with 100ng of total RNA
| | Sample_hyb_protocol | cDNA was fragmented and 10ug hybridized to an Affymetrix U133 Plus 2.0 array for 16 hours. Arrays were washed and stained using the Affy 450 Fluidics Station according to the manufacturer's protocol
| | Sample_scan_protocol | Arrays were scanned using a GeneChip 3000 scanner according to the manufacturers recommendation
| | Sample_data_processing | RMA (Bioconductor Affy Package)
| | Sample_platform_id | GPL570
| | Sample_contact_name | Hu,,Li
| | Sample_contact_email | huli@bu.edu
| | Sample_contact_laboratory | James Collins
| | Sample_contact_institute | Wyss Institute
| | Sample_contact_address | 3 Blackfan Circle
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02115
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM579nnn/GSM579904/suppl/GSM579904.CEL.gz
| | Sample_series_id | GSE23583
| | Sample_data_row_count | 54613
| | |
|
GSM579905 | GPL570 |
|
dH1F_RiPS_1.6
|
RNA iPS
|
cell type: iPS cells derived from dH1F fibroblast
reprogramming protocol: RNA derived iPS
|
Gene expression data obtained from dH1F RNA iPS
|
| Sample_geo_accession | GSM579905
| | Sample_status | Public on Sep 30 2010
| | Sample_submission_date | Aug 16 2010
| | Sample_last_update_date | Sep 27 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | Refer to online method of publication
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol isolation followed by Rneasy (Qiagen) column purification were performed according to manufacturer's specifications
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Preparation of biotinylated cDNA was performed according to the standard Nugen amplification sample preparation protocol, starting with 100ng of total RNA
| | Sample_hyb_protocol | cDNA was fragmented and 10ug hybridized to an Affymetrix U133 Plus 2.0 array for 16 hours. Arrays were washed and stained using the Affy 450 Fluidics Station according to the manufacturer's protocol
| | Sample_scan_protocol | Arrays were scanned using a GeneChip 3000 scanner according to the manufacturers recommendation
| | Sample_data_processing | RMA (Bioconductor Affy Package)
| | Sample_platform_id | GPL570
| | Sample_contact_name | Hu,,Li
| | Sample_contact_email | huli@bu.edu
| | Sample_contact_laboratory | James Collins
| | Sample_contact_institute | Wyss Institute
| | Sample_contact_address | 3 Blackfan Circle
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02115
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM579nnn/GSM579905/suppl/GSM579905.CEL.gz
| | Sample_series_id | GSE23583
| | Sample_data_row_count | 54613
| | |
|
GSM579906 | GPL570 |
|
dH1F_RiPS_1.7
|
RNA iPS
|
cell type: iPS cells derived from dH1F fibroblast
reprogramming protocol: RNA derived iPS
|
Gene expression data obtained from dH1F RNA iPS
|
| Sample_geo_accession | GSM579906
| | Sample_status | Public on Sep 30 2010
| | Sample_submission_date | Aug 16 2010
| | Sample_last_update_date | Sep 27 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | Refer to online method of publication
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol isolation followed by Rneasy (Qiagen) column purification were performed according to manufacturer's specifications
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Preparation of biotinylated cDNA was performed according to the standard Nugen amplification sample preparation protocol, starting with 100ng of total RNA
| | Sample_hyb_protocol | cDNA was fragmented and 10ug hybridized to an Affymetrix U133 Plus 2.0 array for 16 hours. Arrays were washed and stained using the Affy 450 Fluidics Station according to the manufacturer's protocol
| | Sample_scan_protocol | Arrays were scanned using a GeneChip 3000 scanner according to the manufacturers recommendation
| | Sample_data_processing | RMA (Bioconductor Affy Package)
| | Sample_platform_id | GPL570
| | Sample_contact_name | Hu,,Li
| | Sample_contact_email | huli@bu.edu
| | Sample_contact_laboratory | James Collins
| | Sample_contact_institute | Wyss Institute
| | Sample_contact_address | 3 Blackfan Circle
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02115
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM579nnn/GSM579906/suppl/GSM579906.CEL.gz
| | Sample_series_id | GSE23583
| | Sample_data_row_count | 54613
| | |
|
GSM579907 | GPL570 |
|
BJ_RiPS_1.1
|
RNA iPS
|
cell type: iPS cells derived from BJ fibroblast
reprogramming protocol: RNA derived iPS
|
Gene expression data obtained from BJ RNA iPS
|
| Sample_geo_accession | GSM579907
| | Sample_status | Public on Sep 30 2010
| | Sample_submission_date | Aug 16 2010
| | Sample_last_update_date | Sep 27 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | Refer to online method of publication
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol isolation followed by Rneasy (Qiagen) column purification were performed according to manufacturer's specifications
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Preparation of biotinylated cDNA was performed according to the standard Nugen amplification sample preparation protocol, starting with 100ng of total RNA
| | Sample_hyb_protocol | cDNA was fragmented and 10ug hybridized to an Affymetrix U133 Plus 2.0 array for 16 hours. Arrays were washed and stained using the Affy 450 Fluidics Station according to the manufacturer's protocol
| | Sample_scan_protocol | Arrays were scanned using a GeneChip 3000 scanner according to the manufacturers recommendation
| | Sample_data_processing | RMA (Bioconductor Affy Package)
| | Sample_platform_id | GPL570
| | Sample_contact_name | Hu,,Li
| | Sample_contact_email | huli@bu.edu
| | Sample_contact_laboratory | James Collins
| | Sample_contact_institute | Wyss Institute
| | Sample_contact_address | 3 Blackfan Circle
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02115
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM579nnn/GSM579907/suppl/GSM579907.CEL.gz
| | Sample_series_id | GSE23583
| | Sample_data_row_count | 54613
| | |
|
GSM579908 | GPL570 |
|
BJ_RiPS_1.2
|
RNA iPS
|
cell type: iPS cells derived from BJ fibroblast
reprogramming protocol: RNA derived iPS
|
Gene expression data obtained from BJ RNA iPS
|
| Sample_geo_accession | GSM579908
| | Sample_status | Public on Sep 30 2010
| | Sample_submission_date | Aug 16 2010
| | Sample_last_update_date | Sep 27 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | Refer to online method of publication
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol isolation followed by Rneasy (Qiagen) column purification were performed according to manufacturer's specifications
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Preparation of biotinylated cDNA was performed according to the standard Nugen amplification sample preparation protocol, starting with 100ng of total RNA
| | Sample_hyb_protocol | cDNA was fragmented and 10ug hybridized to an Affymetrix U133 Plus 2.0 array for 16 hours. Arrays were washed and stained using the Affy 450 Fluidics Station according to the manufacturer's protocol
| | Sample_scan_protocol | Arrays were scanned using a GeneChip 3000 scanner according to the manufacturers recommendation
| | Sample_data_processing | RMA (Bioconductor Affy Package)
| | Sample_platform_id | GPL570
| | Sample_contact_name | Hu,,Li
| | Sample_contact_email | huli@bu.edu
| | Sample_contact_laboratory | James Collins
| | Sample_contact_institute | Wyss Institute
| | Sample_contact_address | 3 Blackfan Circle
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02115
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM579nnn/GSM579908/suppl/GSM579908.CEL.gz
| | Sample_series_id | GSE23583
| | Sample_data_row_count | 54613
| | |
|
GSM579909 | GPL570 |
|
BJ_RiPS_1.3
|
RNA iPS
|
cell type: iPS cells derived from BJ fibroblast
reprogramming protocol: RNA derived iPS
|
Gene expression data obtained from BJ RNA iPS
|
| Sample_geo_accession | GSM579909
| | Sample_status | Public on Sep 30 2010
| | Sample_submission_date | Aug 16 2010
| | Sample_last_update_date | Sep 27 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | Refer to online method of publication
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol isolation followed by Rneasy (Qiagen) column purification were performed according to manufacturer's specifications
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Preparation of biotinylated cDNA was performed according to the standard Nugen amplification sample preparation protocol, starting with 100ng of total RNA
| | Sample_hyb_protocol | cDNA was fragmented and 10ug hybridized to an Affymetrix U133 Plus 2.0 array for 16 hours. Arrays were washed and stained using the Affy 450 Fluidics Station according to the manufacturer's protocol
| | Sample_scan_protocol | Arrays were scanned using a GeneChip 3000 scanner according to the manufacturers recommendation
| | Sample_data_processing | RMA (Bioconductor Affy Package)
| | Sample_platform_id | GPL570
| | Sample_contact_name | Hu,,Li
| | Sample_contact_email | huli@bu.edu
| | Sample_contact_laboratory | James Collins
| | Sample_contact_institute | Wyss Institute
| | Sample_contact_address | 3 Blackfan Circle
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02115
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM579nnn/GSM579909/suppl/GSM579909.CEL.gz
| | Sample_series_id | GSE23583
| | Sample_data_row_count | 54613
| | |
|
GSM579910 | GPL570 |
|
CF_RiPS_1.2
|
RNA iPS
|
cell type: iPS cells derived from CF fibroblast
reprogramming protocol: RNA derived iPS
|
Gene expression data obtained from CF RNA iPS
|
| Sample_geo_accession | GSM579910
| | Sample_status | Public on Sep 30 2010
| | Sample_submission_date | Aug 16 2010
| | Sample_last_update_date | Sep 27 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | Refer to online method of publication
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol isolation followed by Rneasy (Qiagen) column purification were performed according to manufacturer's specifications
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Preparation of biotinylated cDNA was performed according to the standard Nugen amplification sample preparation protocol, starting with 100ng of total RNA
| | Sample_hyb_protocol | cDNA was fragmented and 10ug hybridized to an Affymetrix U133 Plus 2.0 array for 16 hours. Arrays were washed and stained using the Affy 450 Fluidics Station according to the manufacturer's protocol
| | Sample_scan_protocol | Arrays were scanned using a GeneChip 3000 scanner according to the manufacturers recommendation
| | Sample_data_processing | RMA (Bioconductor Affy Package)
| | Sample_platform_id | GPL570
| | Sample_contact_name | Hu,,Li
| | Sample_contact_email | huli@bu.edu
| | Sample_contact_laboratory | James Collins
| | Sample_contact_institute | Wyss Institute
| | Sample_contact_address | 3 Blackfan Circle
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02115
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM579nnn/GSM579910/suppl/GSM579910.CEL.gz
| | Sample_series_id | GSE23583
| | Sample_data_row_count | 54613
| | |
|
GSM579911 | GPL570 |
|
CF_RiPS_1.3
|
RNA iPS
|
cell type: iPS cells derived from CF fibroblast
reprogramming protocol: RNA derived iPS
|
Gene expression data obtained from CF RNA iPS
|
| Sample_geo_accession | GSM579911
| | Sample_status | Public on Sep 30 2010
| | Sample_submission_date | Aug 16 2010
| | Sample_last_update_date | Sep 27 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | Refer to online method of publication
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol isolation followed by Rneasy (Qiagen) column purification were performed according to manufacturer's specifications
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Preparation of biotinylated cDNA was performed according to the standard Nugen amplification sample preparation protocol, starting with 100ng of total RNA
| | Sample_hyb_protocol | cDNA was fragmented and 10ug hybridized to an Affymetrix U133 Plus 2.0 array for 16 hours. Arrays were washed and stained using the Affy 450 Fluidics Station according to the manufacturer's protocol
| | Sample_scan_protocol | Arrays were scanned using a GeneChip 3000 scanner according to the manufacturers recommendation
| | Sample_data_processing | RMA (Bioconductor Affy Package)
| | Sample_platform_id | GPL570
| | Sample_contact_name | Hu,,Li
| | Sample_contact_email | huli@bu.edu
| | Sample_contact_laboratory | James Collins
| | Sample_contact_institute | Wyss Institute
| | Sample_contact_address | 3 Blackfan Circle
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02115
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM579nnn/GSM579911/suppl/GSM579911.CEL.gz
| | Sample_series_id | GSE23583
| | Sample_data_row_count | 54613
| | |
|
GSM579912 | GPL570 |
|
CF_RiPS_1.4
|
RNA iPS
|
cell type: iPS cells derived from CF fibroblast
reprogramming protocol: RNA derived iPS
|
Gene expression data obtained from CF RNA iPS
|
| Sample_geo_accession | GSM579912
| | Sample_status | Public on Sep 30 2010
| | Sample_submission_date | Aug 16 2010
| | Sample_last_update_date | Sep 27 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | Refer to online method of publication
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol isolation followed by Rneasy (Qiagen) column purification were performed according to manufacturer's specifications
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Preparation of biotinylated cDNA was performed according to the standard Nugen amplification sample preparation protocol, starting with 100ng of total RNA
| | Sample_hyb_protocol | cDNA was fragmented and 10ug hybridized to an Affymetrix U133 Plus 2.0 array for 16 hours. Arrays were washed and stained using the Affy 450 Fluidics Station according to the manufacturer's protocol
| | Sample_scan_protocol | Arrays were scanned using a GeneChip 3000 scanner according to the manufacturers recommendation
| | Sample_data_processing | RMA (Bioconductor Affy Package)
| | Sample_platform_id | GPL570
| | Sample_contact_name | Hu,,Li
| | Sample_contact_email | huli@bu.edu
| | Sample_contact_laboratory | James Collins
| | Sample_contact_institute | Wyss Institute
| | Sample_contact_address | 3 Blackfan Circle
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02115
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM579nnn/GSM579912/suppl/GSM579912.CEL.gz
| | Sample_series_id | GSE23583
| | Sample_data_row_count | 54613
| | |
|
GSM579913 | GPL570 |
|
MRC5_RiPS_1.8
|
RNA iPS
|
cell type: iPS cells derived from MRC5 fibroblast
reprogramming protocol: RNA derived iPS
|
Gene expression data obtained from MRC5 RNA iPS
|
| Sample_geo_accession | GSM579913
| | Sample_status | Public on Sep 30 2010
| | Sample_submission_date | Aug 16 2010
| | Sample_last_update_date | Sep 27 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | Refer to online method of publication
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol isolation followed by Rneasy (Qiagen) column purification were performed according to manufacturer's specifications
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Preparation of biotinylated cDNA was performed according to the standard Nugen amplification sample preparation protocol, starting with 100ng of total RNA
| | Sample_hyb_protocol | cDNA was fragmented and 10ug hybridized to an Affymetrix U133 Plus 2.0 array for 16 hours. Arrays were washed and stained using the Affy 450 Fluidics Station according to the manufacturer's protocol
| | Sample_scan_protocol | Arrays were scanned using a GeneChip 3000 scanner according to the manufacturers recommendation
| | Sample_data_processing | RMA (Bioconductor Affy Package)
| | Sample_platform_id | GPL570
| | Sample_contact_name | Hu,,Li
| | Sample_contact_email | huli@bu.edu
| | Sample_contact_laboratory | James Collins
| | Sample_contact_institute | Wyss Institute
| | Sample_contact_address | 3 Blackfan Circle
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02115
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM579nnn/GSM579913/suppl/GSM579913.CEL.gz
| | Sample_series_id | GSE23583
| | Sample_data_row_count | 54613
| | |
|
GSM579914 | GPL570 |
|
MRC5_RiPS_1.9
|
RNA iPS
|
cell type: iPS cells derived from MRC5 fibroblast
reprogramming protocol: RNA derived iPS
|
Gene expression data obtained from MRC5 RNA iPS
|
| Sample_geo_accession | GSM579914
| | Sample_status | Public on Sep 30 2010
| | Sample_submission_date | Aug 16 2010
| | Sample_last_update_date | Sep 27 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | Refer to online method of publication
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol isolation followed by Rneasy (Qiagen) column purification were performed according to manufacturer's specifications
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Preparation of biotinylated cDNA was performed according to the standard Nugen amplification sample preparation protocol, starting with 100ng of total RNA
| | Sample_hyb_protocol | cDNA was fragmented and 10ug hybridized to an Affymetrix U133 Plus 2.0 array for 16 hours. Arrays were washed and stained using the Affy 450 Fluidics Station according to the manufacturer's protocol
| | Sample_scan_protocol | Arrays were scanned using a GeneChip 3000 scanner according to the manufacturers recommendation
| | Sample_data_processing | RMA (Bioconductor Affy Package)
| | Sample_platform_id | GPL570
| | Sample_contact_name | Hu,,Li
| | Sample_contact_email | huli@bu.edu
| | Sample_contact_laboratory | James Collins
| | Sample_contact_institute | Wyss Institute
| | Sample_contact_address | 3 Blackfan Circle
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02115
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM579nnn/GSM579914/suppl/GSM579914.CEL.gz
| | Sample_series_id | GSE23583
| | Sample_data_row_count | 54613
| | |
|
GSM579915 | GPL570 |
|
MRC5_RiPS_1.11
|
RNA iPS
|
cell type: iPS cells derived from MRC5 fibroblast
reprogramming protocol: RNA derived iPS
|
Gene expression data obtained from MRC5 RNA iPS
|
| Sample_geo_accession | GSM579915
| | Sample_status | Public on Sep 30 2010
| | Sample_submission_date | Aug 16 2010
| | Sample_last_update_date | Sep 27 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | Refer to online method of publication
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol isolation followed by Rneasy (Qiagen) column purification were performed according to manufacturer's specifications
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Preparation of biotinylated cDNA was performed according to the standard Nugen amplification sample preparation protocol, starting with 100ng of total RNA
| | Sample_hyb_protocol | cDNA was fragmented and 10ug hybridized to an Affymetrix U133 Plus 2.0 array for 16 hours. Arrays were washed and stained using the Affy 450 Fluidics Station according to the manufacturer's protocol
| | Sample_scan_protocol | Arrays were scanned using a GeneChip 3000 scanner according to the manufacturers recommendation
| | Sample_data_processing | RMA (Bioconductor Affy Package)
| | Sample_platform_id | GPL570
| | Sample_contact_name | Hu,,Li
| | Sample_contact_email | huli@bu.edu
| | Sample_contact_laboratory | James Collins
| | Sample_contact_institute | Wyss Institute
| | Sample_contact_address | 3 Blackfan Circle
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02115
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM579nnn/GSM579915/suppl/GSM579915.CEL.gz
| | Sample_series_id | GSE23583
| | Sample_data_row_count | 54613
| | |
|
GSM579916 | GPL570 |
|
BJ_Fibroblast_Untransfected
|
fibroblast, untransfected
|
cell type: BJ fibroblast
reprogramming protocol: n/a
|
Gene expression data obtained from fibroblasts
|
| Sample_geo_accession | GSM579916
| | Sample_status | Public on Sep 30 2010
| | Sample_submission_date | Aug 16 2010
| | Sample_last_update_date | Sep 27 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | Fibroblasts were grown in alpha-MEM containing 10 % inactivated fetal serum (IFS), 50 U/ml penicillin, 50 mg/ml streptomycin, and 1 mM L-glutamine. Replacement,10 ng/ml bFGF, 1 mM L-glutamine, 100 uM nonessential amino acids, 100 uM 2-mercaptoethanol, 50 U/ml penicillin, and 50 mg/ml streptomycin).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol isolation followed by Rneasy (Qiagen) column purification were performed according to manufacturer's specifications
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Preparation of biotinylated cDNA was performed according to the standard Nugen amplification sample preparation protocol, starting with 100ng of total RNA
| | Sample_hyb_protocol | cDNA was fragmented and 10ug hybridized to an Affymetrix U133 Plus 2.0 array for 16 hours. Arrays were washed and stained using the Affy 450 Fluidics Station according to the manufacturer's protocol
| | Sample_scan_protocol | Arrays were scanned using a GeneChip 3000 scanner according to the manufacturers recommendation
| | Sample_data_processing | RMA (Bioconductor Affy Package)
| | Sample_platform_id | GPL570
| | Sample_contact_name | Hu,,Li
| | Sample_contact_email | huli@bu.edu
| | Sample_contact_laboratory | James Collins
| | Sample_contact_institute | Wyss Institute
| | Sample_contact_address | 3 Blackfan Circle
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02115
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM579nnn/GSM579916/suppl/GSM579916.CEL.gz
| | Sample_series_id | GSE23583
| | Sample_data_row_count | 54613
| | |
|
GSM579917 | GPL570 |
|
BJ_Fibroblast_Vehicle
|
fibroblast, vehicle-transfected
|
cell type: BJ fibroblast
reprogramming protocol: vehicle control
|
Gene expression data obtained from fibroblasts
|
| Sample_geo_accession | GSM579917
| | Sample_status | Public on Sep 30 2010
| | Sample_submission_date | Aug 16 2010
| | Sample_last_update_date | Sep 27 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | Fibroblasts were grown in alpha-MEM containing 10 % inactivated fetal serum (IFS), 50 U/ml penicillin, 50 mg/ml streptomycin, and 1 mM L-glutamine. Replacement,10 ng/ml bFGF, 1 mM L-glutamine, 100 uM nonessential amino acids, 100 uM 2-mercaptoethanol, 50 U/ml penicillin, and 50 mg/ml streptomycin).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol isolation followed by Rneasy (Qiagen) column purification were performed according to manufacturer's specifications
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Preparation of biotinylated cDNA was performed according to the standard Nugen amplification sample preparation protocol, starting with 100ng of total RNA
| | Sample_hyb_protocol | cDNA was fragmented and 10ug hybridized to an Affymetrix U133 Plus 2.0 array for 16 hours. Arrays were washed and stained using the Affy 450 Fluidics Station according to the manufacturer's protocol
| | Sample_scan_protocol | Arrays were scanned using a GeneChip 3000 scanner according to the manufacturers recommendation
| | Sample_data_processing | RMA (Bioconductor Affy Package)
| | Sample_platform_id | GPL570
| | Sample_contact_name | Hu,,Li
| | Sample_contact_email | huli@bu.edu
| | Sample_contact_laboratory | James Collins
| | Sample_contact_institute | Wyss Institute
| | Sample_contact_address | 3 Blackfan Circle
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02115
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM579nnn/GSM579917/suppl/GSM579917.CEL.gz
| | Sample_series_id | GSE23583
| | Sample_data_row_count | 54613
| | |
|
GSM579918 | GPL570 |
|
BJ_Fibroblast_Modified-RNA
|
fibroblast, modified-RNA transfected
|
cell type: BJ fibroblast
reprogramming protocol: modified-RNA (i.e., synthetic mRNA with GFP)
|
Gene expression data obtained from fibroblasts
|
| Sample_geo_accession | GSM579918
| | Sample_status | Public on Sep 30 2010
| | Sample_submission_date | Aug 16 2010
| | Sample_last_update_date | Sep 27 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | Fibroblasts were grown in alpha-MEM containing 10 % inactivated fetal serum (IFS), 50 U/ml penicillin, 50 mg/ml streptomycin, and 1 mM L-glutamine. Replacement,10 ng/ml bFGF, 1 mM L-glutamine, 100 uM nonessential amino acids, 100 uM 2-mercaptoethanol, 50 U/ml penicillin, and 50 mg/ml streptomycin).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol isolation followed by Rneasy (Qiagen) column purification were performed according to manufacturer's specifications
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Preparation of biotinylated cDNA was performed according to the standard Nugen amplification sample preparation protocol, starting with 100ng of total RNA
| | Sample_hyb_protocol | cDNA was fragmented and 10ug hybridized to an Affymetrix U133 Plus 2.0 array for 16 hours. Arrays were washed and stained using the Affy 450 Fluidics Station according to the manufacturer's protocol
| | Sample_scan_protocol | Arrays were scanned using a GeneChip 3000 scanner according to the manufacturers recommendation
| | Sample_data_processing | RMA (Bioconductor Affy Package)
| | Sample_platform_id | GPL570
| | Sample_contact_name | Hu,,Li
| | Sample_contact_email | huli@bu.edu
| | Sample_contact_laboratory | James Collins
| | Sample_contact_institute | Wyss Institute
| | Sample_contact_address | 3 Blackfan Circle
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02115
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM579nnn/GSM579918/suppl/GSM579918.CEL.gz
| | Sample_series_id | GSE23583
| | Sample_data_row_count | 54613
| | |
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