Search results for the GEO ID: GSE23600  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM578844 | GPL1261 |
|
T regs from WT mouse, biological rep 1
|
T regs from WT mouse
|
cell type: T reg (CD4+CD25+) cells
strain: BALB/c
genotype/variation: wt
|
|
| Sample_geo_accession | GSM578844
| | Sample_status | Public on Aug 13 2010
| | Sample_submission_date | Aug 12 2010
| | Sample_last_update_date | Aug 13 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Cells were not treated or cultured after harvest
| | Sample_growth_protocol_ch1 | CD4+CD25+ T cells were isolated from lymph nodes (combined inguinal, axillary, brachial, cervical, lumbar, sacral, renal and pancreatic nodes unless otherwise indicated) of 17-19 day old mice using the Regulatory T cell Isolation kit (Miltenyi Biotec; Auburn, CA) according to the manufacturer’s protocol
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA extraction was performed using the Arcturus PicoPuire kit, per manufacturer's instructions; aRNA was generated and amplified using the Arcturus RiboAmp kit, per manufacturer's instructions, from 20ng of total RNA.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotin-labeled cRNA was generated using the Affymetrix GeneChip IVT Labeling kit, per manufacturer's instructions, using 10ug of aRNA
| | Sample_hyb_protocol | Following fragmentation, 10ug of cRNA were hybridized for 16hr on the Affymetrix Mouse 2.0 GeneChip and processed in an Affymetrix Fluidics Station, per manufacturer's instructions.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett Packard GeneArray Laser Scanner system
| | Sample_data_processing | Data were analyzed with DNAStar ArrayStar 3.0.2 build 38 using global scaling normalization.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | David,W,Mullins
| | Sample_contact_email | dmullins@virginia.edu
| | Sample_contact_phone | 434 924 5112
| | Sample_contact_department | Microbiology
| | Sample_contact_institute | University of Virginia
| | Sample_contact_address | 345 Crispell Dr. Rm G525
| | Sample_contact_city | Charlottesville
| | Sample_contact_state | VA
| | Sample_contact_zip/postal_code | 22908
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM578nnn/GSM578844/suppl/GSM578844.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM578nnn/GSM578844/suppl/GSM578844.chp.gz
| | Sample_series_id | GSE23600
| | Sample_data_row_count | 45101
| | |
|
GSM578845 | GPL1261 |
|
T regs from WT mouse, biological rep 2
|
T regs from WT mouse
|
cell type: T reg (CD4+CD25+) cells
strain: BALB/c
genotype/variation: wt
|
|
| Sample_geo_accession | GSM578845
| | Sample_status | Public on Aug 13 2010
| | Sample_submission_date | Aug 12 2010
| | Sample_last_update_date | Aug 13 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Cells were not treated or cultured after harvest
| | Sample_growth_protocol_ch1 | CD4+CD25+ T cells were isolated from lymph nodes (combined inguinal, axillary, brachial, cervical, lumbar, sacral, renal and pancreatic nodes unless otherwise indicated) of 17-19 day old mice using the Regulatory T cell Isolation kit (Miltenyi Biotec; Auburn, CA) according to the manufacturer’s protocol
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA extraction was performed using the Arcturus PicoPuire kit, per manufacturer's instructions; aRNA was generated and amplified using the Arcturus RiboAmp kit, per manufacturer's instructions, from 20ng of total RNA.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotin-labeled cRNA was generated using the Affymetrix GeneChip IVT Labeling kit, per manufacturer's instructions, using 10ug of aRNA
| | Sample_hyb_protocol | Following fragmentation, 10ug of cRNA were hybridized for 16hr on the Affymetrix Mouse 2.0 GeneChip and processed in an Affymetrix Fluidics Station, per manufacturer's instructions.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett Packard GeneArray Laser Scanner system
| | Sample_data_processing | Data were analyzed with DNAStar ArrayStar 3.0.2 build 38 using global scaling normalization.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | David,W,Mullins
| | Sample_contact_email | dmullins@virginia.edu
| | Sample_contact_phone | 434 924 5112
| | Sample_contact_department | Microbiology
| | Sample_contact_institute | University of Virginia
| | Sample_contact_address | 345 Crispell Dr. Rm G525
| | Sample_contact_city | Charlottesville
| | Sample_contact_state | VA
| | Sample_contact_zip/postal_code | 22908
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM578nnn/GSM578845/suppl/GSM578845.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM578nnn/GSM578845/suppl/GSM578845.chp.gz
| | Sample_series_id | GSE23600
| | Sample_data_row_count | 45101
| | |
|
GSM578846 | GPL1261 |
|
T regs from WT mouse, biological rep 3
|
T regs from WT mouse
|
cell type: T reg (CD4+CD25+) cells
strain: BALB/c
genotype/variation: wt
|
|
| Sample_geo_accession | GSM578846
| | Sample_status | Public on Aug 13 2010
| | Sample_submission_date | Aug 12 2010
| | Sample_last_update_date | Aug 13 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Cells were not treated or cultured after harvest
| | Sample_growth_protocol_ch1 | CD4+CD25+ T cells were isolated from lymph nodes (combined inguinal, axillary, brachial, cervical, lumbar, sacral, renal and pancreatic nodes unless otherwise indicated) of 17-19 day old mice using the Regulatory T cell Isolation kit (Miltenyi Biotec; Auburn, CA) according to the manufacturer’s protocol
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA extraction was performed using the Arcturus PicoPuire kit, per manufacturer's instructions; aRNA was generated and amplified using the Arcturus RiboAmp kit, per manufacturer's instructions, from 20ng of total RNA.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotin-labeled cRNA was generated using the Affymetrix GeneChip IVT Labeling kit, per manufacturer's instructions, using 10ug of aRNA
| | Sample_hyb_protocol | Following fragmentation, 10ug of cRNA were hybridized for 16hr on the Affymetrix Mouse 2.0 GeneChip and processed in an Affymetrix Fluidics Station, per manufacturer's instructions.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett Packard GeneArray Laser Scanner system
| | Sample_data_processing | Data were analyzed with DNAStar ArrayStar 3.0.2 build 38 using global scaling normalization.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | David,W,Mullins
| | Sample_contact_email | dmullins@virginia.edu
| | Sample_contact_phone | 434 924 5112
| | Sample_contact_department | Microbiology
| | Sample_contact_institute | University of Virginia
| | Sample_contact_address | 345 Crispell Dr. Rm G525
| | Sample_contact_city | Charlottesville
| | Sample_contact_state | VA
| | Sample_contact_zip/postal_code | 22908
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM578nnn/GSM578846/suppl/GSM578846.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM578nnn/GSM578846/suppl/GSM578846.chp.gz
| | Sample_series_id | GSE23600
| | Sample_data_row_count | 45101
| | |
|
GSM578847 | GPL1261 |
|
T regs from WT mouse, biological rep 4
|
T regs from WT mouse
|
cell type: T reg (CD4+CD25+) cells
strain: BALB/c
genotype/variation: wt
|
|
| Sample_geo_accession | GSM578847
| | Sample_status | Public on Aug 13 2010
| | Sample_submission_date | Aug 12 2010
| | Sample_last_update_date | Aug 13 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Cells were not treated or cultured after harvest
| | Sample_growth_protocol_ch1 | CD4+CD25+ T cells were isolated from lymph nodes (combined inguinal, axillary, brachial, cervical, lumbar, sacral, renal and pancreatic nodes unless otherwise indicated) of 17-19 day old mice using the Regulatory T cell Isolation kit (Miltenyi Biotec; Auburn, CA) according to the manufacturer’s protocol
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA extraction was performed using the Arcturus PicoPuire kit, per manufacturer's instructions; aRNA was generated and amplified using the Arcturus RiboAmp kit, per manufacturer's instructions, from 20ng of total RNA.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotin-labeled cRNA was generated using the Affymetrix GeneChip IVT Labeling kit, per manufacturer's instructions, using 10ug of aRNA
| | Sample_hyb_protocol | Following fragmentation, 10ug of cRNA were hybridized for 16hr on the Affymetrix Mouse 2.0 GeneChip and processed in an Affymetrix Fluidics Station, per manufacturer's instructions.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett Packard GeneArray Laser Scanner system
| | Sample_data_processing | Data were analyzed with DNAStar ArrayStar 3.0.2 build 38 using global scaling normalization.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | David,W,Mullins
| | Sample_contact_email | dmullins@virginia.edu
| | Sample_contact_phone | 434 924 5112
| | Sample_contact_department | Microbiology
| | Sample_contact_institute | University of Virginia
| | Sample_contact_address | 345 Crispell Dr. Rm G525
| | Sample_contact_city | Charlottesville
| | Sample_contact_state | VA
| | Sample_contact_zip/postal_code | 22908
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM578nnn/GSM578847/suppl/GSM578847.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM578nnn/GSM578847/suppl/GSM578847.chp.gz
| | Sample_series_id | GSE23600
| | Sample_data_row_count | 45101
| | |
|
GSM578848 | GPL1261 |
|
T regs from mouse heterozygous for SHP-1 knockout, biological rep 1
|
T regs from mouse heterozygous for SHP-1 knockout
|
cell type: T reg (CD4+CD25+) cells
strain: BALB/c
genotype/variation: heterozygous (he) for SHP-1 knockout
|
|
| Sample_geo_accession | GSM578848
| | Sample_status | Public on Aug 13 2010
| | Sample_submission_date | Aug 12 2010
| | Sample_last_update_date | Aug 13 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Cells were not treated or cultured after harvest
| | Sample_growth_protocol_ch1 | CD4+CD25+ T cells were isolated from lymph nodes (combined inguinal, axillary, brachial, cervical, lumbar, sacral, renal and pancreatic nodes unless otherwise indicated) of 17-19 day old mice using the Regulatory T cell Isolation kit (Miltenyi Biotec; Auburn, CA) according to the manufacturer’s protocol
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA extraction was performed using the Arcturus PicoPuire kit, per manufacturer's instructions; aRNA was generated and amplified using the Arcturus RiboAmp kit, per manufacturer's instructions, from 20ng of total RNA.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotin-labeled cRNA was generated using the Affymetrix GeneChip IVT Labeling kit, per manufacturer's instructions, using 10ug of aRNA
| | Sample_hyb_protocol | Following fragmentation, 10ug of cRNA were hybridized for 16hr on the Affymetrix Mouse 2.0 GeneChip and processed in an Affymetrix Fluidics Station, per manufacturer's instructions.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett Packard GeneArray Laser Scanner system
| | Sample_data_processing | Data were analyzed with DNAStar ArrayStar 3.0.2 build 38 using global scaling normalization.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | David,W,Mullins
| | Sample_contact_email | dmullins@virginia.edu
| | Sample_contact_phone | 434 924 5112
| | Sample_contact_department | Microbiology
| | Sample_contact_institute | University of Virginia
| | Sample_contact_address | 345 Crispell Dr. Rm G525
| | Sample_contact_city | Charlottesville
| | Sample_contact_state | VA
| | Sample_contact_zip/postal_code | 22908
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM578nnn/GSM578848/suppl/GSM578848.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM578nnn/GSM578848/suppl/GSM578848.chp.gz
| | Sample_series_id | GSE23600
| | Sample_data_row_count | 45101
| | |
|
GSM578849 | GPL1261 |
|
T regs from mouse heterozygous for SHP-1 knockout, biological rep 2
|
T regs from mouse heterozygous for SHP-1 knockout
|
cell type: T reg (CD4+CD25+) cells
strain: BALB/c
genotype/variation: heterozygous (he) for SHP-1 knockout
|
|
| Sample_geo_accession | GSM578849
| | Sample_status | Public on Aug 13 2010
| | Sample_submission_date | Aug 12 2010
| | Sample_last_update_date | Aug 13 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Cells were not treated or cultured after harvest
| | Sample_growth_protocol_ch1 | CD4+CD25+ T cells were isolated from lymph nodes (combined inguinal, axillary, brachial, cervical, lumbar, sacral, renal and pancreatic nodes unless otherwise indicated) of 17-19 day old mice using the Regulatory T cell Isolation kit (Miltenyi Biotec; Auburn, CA) according to the manufacturer’s protocol
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA extraction was performed using the Arcturus PicoPuire kit, per manufacturer's instructions; aRNA was generated and amplified using the Arcturus RiboAmp kit, per manufacturer's instructions, from 20ng of total RNA.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotin-labeled cRNA was generated using the Affymetrix GeneChip IVT Labeling kit, per manufacturer's instructions, using 10ug of aRNA
| | Sample_hyb_protocol | Following fragmentation, 10ug of cRNA were hybridized for 16hr on the Affymetrix Mouse 2.0 GeneChip and processed in an Affymetrix Fluidics Station, per manufacturer's instructions.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett Packard GeneArray Laser Scanner system
| | Sample_data_processing | Data were analyzed with DNAStar ArrayStar 3.0.2 build 38 using global scaling normalization.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | David,W,Mullins
| | Sample_contact_email | dmullins@virginia.edu
| | Sample_contact_phone | 434 924 5112
| | Sample_contact_department | Microbiology
| | Sample_contact_institute | University of Virginia
| | Sample_contact_address | 345 Crispell Dr. Rm G525
| | Sample_contact_city | Charlottesville
| | Sample_contact_state | VA
| | Sample_contact_zip/postal_code | 22908
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM578nnn/GSM578849/suppl/GSM578849.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM578nnn/GSM578849/suppl/GSM578849.chp.gz
| | Sample_series_id | GSE23600
| | Sample_data_row_count | 45101
| | |
|
GSM578850 | GPL1261 |
|
T regs from mouse heterozygous for SHP-1 knockout, biological rep 3
|
T regs from mouse heterozygous for SHP-1 knockout
|
cell type: T reg (CD4+CD25+) cells
strain: BALB/c
genotype/variation: heterozygous (he) for SHP-1 knockout
|
|
| Sample_geo_accession | GSM578850
| | Sample_status | Public on Aug 13 2010
| | Sample_submission_date | Aug 12 2010
| | Sample_last_update_date | Aug 13 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Cells were not treated or cultured after harvest
| | Sample_growth_protocol_ch1 | CD4+CD25+ T cells were isolated from lymph nodes (combined inguinal, axillary, brachial, cervical, lumbar, sacral, renal and pancreatic nodes unless otherwise indicated) of 17-19 day old mice using the Regulatory T cell Isolation kit (Miltenyi Biotec; Auburn, CA) according to the manufacturer’s protocol
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA extraction was performed using the Arcturus PicoPuire kit, per manufacturer's instructions; aRNA was generated and amplified using the Arcturus RiboAmp kit, per manufacturer's instructions, from 20ng of total RNA.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotin-labeled cRNA was generated using the Affymetrix GeneChip IVT Labeling kit, per manufacturer's instructions, using 10ug of aRNA
| | Sample_hyb_protocol | Following fragmentation, 10ug of cRNA were hybridized for 16hr on the Affymetrix Mouse 2.0 GeneChip and processed in an Affymetrix Fluidics Station, per manufacturer's instructions.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett Packard GeneArray Laser Scanner system
| | Sample_data_processing | Data were analyzed with DNAStar ArrayStar 3.0.2 build 38 using global scaling normalization.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | David,W,Mullins
| | Sample_contact_email | dmullins@virginia.edu
| | Sample_contact_phone | 434 924 5112
| | Sample_contact_department | Microbiology
| | Sample_contact_institute | University of Virginia
| | Sample_contact_address | 345 Crispell Dr. Rm G525
| | Sample_contact_city | Charlottesville
| | Sample_contact_state | VA
| | Sample_contact_zip/postal_code | 22908
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM578nnn/GSM578850/suppl/GSM578850.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM578nnn/GSM578850/suppl/GSM578850.chp.gz
| | Sample_series_id | GSE23600
| | Sample_data_row_count | 45101
| | |
|
GSM578851 | GPL1261 |
|
T regs from mouse homozygous for SHP-1 knockout, biological rep 1
|
T regs from mouse homozygous for SHP-1 knockout
|
cell type: T reg (CD4+CD25+) cells
strain: BALB/c
genotype/variation: homozygous (me) for SHP-1 knockout (motheaten phenotype)
|
|
| Sample_geo_accession | GSM578851
| | Sample_status | Public on Aug 13 2010
| | Sample_submission_date | Aug 12 2010
| | Sample_last_update_date | Aug 13 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Cells were not treated or cultured after harvest
| | Sample_growth_protocol_ch1 | CD4+CD25+ T cells were isolated from lymph nodes (combined inguinal, axillary, brachial, cervical, lumbar, sacral, renal and pancreatic nodes unless otherwise indicated) of 17-19 day old mice using the Regulatory T cell Isolation kit (Miltenyi Biotec; Auburn, CA) according to the manufacturer’s protocol
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA extraction was performed using the Arcturus PicoPuire kit, per manufacturer's instructions; aRNA was generated and amplified using the Arcturus RiboAmp kit, per manufacturer's instructions, from 20ng of total RNA.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotin-labeled cRNA was generated using the Affymetrix GeneChip IVT Labeling kit, per manufacturer's instructions, using 10ug of aRNA
| | Sample_hyb_protocol | Following fragmentation, 10ug of cRNA were hybridized for 16hr on the Affymetrix Mouse 2.0 GeneChip and processed in an Affymetrix Fluidics Station, per manufacturer's instructions.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett Packard GeneArray Laser Scanner system
| | Sample_data_processing | Data were analyzed with DNAStar ArrayStar 3.0.2 build 38 using global scaling normalization.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | David,W,Mullins
| | Sample_contact_email | dmullins@virginia.edu
| | Sample_contact_phone | 434 924 5112
| | Sample_contact_department | Microbiology
| | Sample_contact_institute | University of Virginia
| | Sample_contact_address | 345 Crispell Dr. Rm G525
| | Sample_contact_city | Charlottesville
| | Sample_contact_state | VA
| | Sample_contact_zip/postal_code | 22908
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM578nnn/GSM578851/suppl/GSM578851.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM578nnn/GSM578851/suppl/GSM578851.chp.gz
| | Sample_series_id | GSE23600
| | Sample_data_row_count | 45101
| | |
|
GSM578852 | GPL1261 |
|
T regs from mouse homozygous for SHP-1 knockout, biological rep 2
|
T regs from mouse homozygous for SHP-1 knockout
|
cell type: T reg (CD4+CD25+) cells
strain: BALB/c
genotype/variation: homozygous (me) for SHP-1 knockout (motheaten phenotype)
|
|
| Sample_geo_accession | GSM578852
| | Sample_status | Public on Aug 13 2010
| | Sample_submission_date | Aug 12 2010
| | Sample_last_update_date | Aug 13 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Cells were not treated or cultured after harvest
| | Sample_growth_protocol_ch1 | CD4+CD25+ T cells were isolated from lymph nodes (combined inguinal, axillary, brachial, cervical, lumbar, sacral, renal and pancreatic nodes unless otherwise indicated) of 17-19 day old mice using the Regulatory T cell Isolation kit (Miltenyi Biotec; Auburn, CA) according to the manufacturer’s protocol
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA extraction was performed using the Arcturus PicoPuire kit, per manufacturer's instructions; aRNA was generated and amplified using the Arcturus RiboAmp kit, per manufacturer's instructions, from 20ng of total RNA.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotin-labeled cRNA was generated using the Affymetrix GeneChip IVT Labeling kit, per manufacturer's instructions, using 10ug of aRNA
| | Sample_hyb_protocol | Following fragmentation, 10ug of cRNA were hybridized for 16hr on the Affymetrix Mouse 2.0 GeneChip and processed in an Affymetrix Fluidics Station, per manufacturer's instructions.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett Packard GeneArray Laser Scanner system
| | Sample_data_processing | Data were analyzed with DNAStar ArrayStar 3.0.2 build 38 using global scaling normalization.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | David,W,Mullins
| | Sample_contact_email | dmullins@virginia.edu
| | Sample_contact_phone | 434 924 5112
| | Sample_contact_department | Microbiology
| | Sample_contact_institute | University of Virginia
| | Sample_contact_address | 345 Crispell Dr. Rm G525
| | Sample_contact_city | Charlottesville
| | Sample_contact_state | VA
| | Sample_contact_zip/postal_code | 22908
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM578nnn/GSM578852/suppl/GSM578852.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM578nnn/GSM578852/suppl/GSM578852.chp.gz
| | Sample_series_id | GSE23600
| | Sample_data_row_count | 45101
| | |
|
GSM578853 | GPL1261 |
|
T regs from mouse homozygous for SHP-1 knockout, biological rep 3
|
T regs from mouse homozygous for SHP-1 knockout
|
cell type: T reg (CD4+CD25+) cells
strain: BALB/c
genotype/variation: homozygous (me) for SHP-1 knockout (motheaten phenotype)
|
|
| Sample_geo_accession | GSM578853
| | Sample_status | Public on Aug 13 2010
| | Sample_submission_date | Aug 12 2010
| | Sample_last_update_date | Aug 13 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Cells were not treated or cultured after harvest
| | Sample_growth_protocol_ch1 | CD4+CD25+ T cells were isolated from lymph nodes (combined inguinal, axillary, brachial, cervical, lumbar, sacral, renal and pancreatic nodes unless otherwise indicated) of 17-19 day old mice using the Regulatory T cell Isolation kit (Miltenyi Biotec; Auburn, CA) according to the manufacturer’s protocol
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA extraction was performed using the Arcturus PicoPuire kit, per manufacturer's instructions; aRNA was generated and amplified using the Arcturus RiboAmp kit, per manufacturer's instructions, from 20ng of total RNA.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotin-labeled cRNA was generated using the Affymetrix GeneChip IVT Labeling kit, per manufacturer's instructions, using 10ug of aRNA
| | Sample_hyb_protocol | Following fragmentation, 10ug of cRNA were hybridized for 16hr on the Affymetrix Mouse 2.0 GeneChip and processed in an Affymetrix Fluidics Station, per manufacturer's instructions.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett Packard GeneArray Laser Scanner system
| | Sample_data_processing | Data were analyzed with DNAStar ArrayStar 3.0.2 build 38 using global scaling normalization.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | David,W,Mullins
| | Sample_contact_email | dmullins@virginia.edu
| | Sample_contact_phone | 434 924 5112
| | Sample_contact_department | Microbiology
| | Sample_contact_institute | University of Virginia
| | Sample_contact_address | 345 Crispell Dr. Rm G525
| | Sample_contact_city | Charlottesville
| | Sample_contact_state | VA
| | Sample_contact_zip/postal_code | 22908
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM578nnn/GSM578853/suppl/GSM578853.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM578nnn/GSM578853/suppl/GSM578853.chp.gz
| | Sample_series_id | GSE23600
| | Sample_data_row_count | 45101
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