Search results for the GEO ID: GSE23610  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM578941 | GPL570 |
|
MCF-7_0.001%DMSO_rep1
|
Human breast cancer cell line MCF-7
|
cell line: breast cancer cell line MCF-7
treatment: DMSO treated
|
Gene expression profiling data from human breast cancer cell line MCF-7
|
| Sample_geo_accession | GSM578941
| | Sample_status | Public on May 18 2011
| | Sample_submission_date | Aug 13 2010
| | Sample_last_update_date | May 18 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The MCF-7 cells were incubated with hormone free medium and treated by 0.001% DMSO (control group), 0.1 µM 17 β-estradiol, 0.1, 1 or 10 µM of ferulic acid (equivalent to 0.0194, 0.194, and 1.94 mg/ml), 0.0256, 0.256, and 2.56 mg/ml SWT for 6 hours.
| | Sample_growth_protocol_ch1 | The MCF-7 cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% fetal bovine serum (FBS), 1% non-essential amino acids, 100 unit/mL penicillin, 100 μg/mL streptomycin, 1 mM sodium pyruvate, and 2 mM L-glutamine in an atmosphere of 5% CO2 at 37 °C.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted using QIAGEN RNeasy Mini Kit, following the manufacturer’s protocol.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA samples were prepared according to the standard Affymetrix GeneChip protocol.
| | Sample_hyb_protocol | For each sample, 10 µg of biotinylated cRNA spiked with bioB, bioC, bioD and cre (Hybridization Control) was hybridized to a Human Genome U133 Plus 2.0 microarray for 16 hours at 45ºC.
| | Sample_scan_protocol | GeneChips were scanned with an Affymetrix GeneChip® Scanner 3000.
| | Sample_data_processing | The probeset-level expression data were summarized from probe-level data with Robust Multiarray Averaging method (RMA) by taking all 24 microarrays together.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Leming,,Shi
| | Sample_contact_email | leming.shi@gmail.com
| | Sample_contact_phone | 870-543-7387
| | Sample_contact_department | Systems Toxicology
| | Sample_contact_institute | NCTR/FDA
| | Sample_contact_address | 3900 NCTR Road
| | Sample_contact_city | Jefferson
| | Sample_contact_state | AR
| | Sample_contact_zip/postal_code | 72079
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM578nnn/GSM578941/suppl/GSM578941.CEL.gz
| | Sample_series_id | GSE23610
| | Sample_data_row_count | 54675
| | |
|
GSM578942 | GPL570 |
|
MCF-7_0.001%DMSO_rep2
|
Human breast cancer cell line MCF-7
|
cell line: breast cancer cell line MCF-7
treatment: DMSO treated
|
Gene expression profiling data from human breast cancer cell line MCF-7
|
| Sample_geo_accession | GSM578942
| | Sample_status | Public on May 18 2011
| | Sample_submission_date | Aug 13 2010
| | Sample_last_update_date | May 18 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The MCF-7 cells were incubated with hormone free medium and treated by 0.001% DMSO (control group), 0.1 µM 17 β-estradiol, 0.1, 1 or 10 µM of ferulic acid (equivalent to 0.0194, 0.194, and 1.94 mg/ml), 0.0256, 0.256, and 2.56 mg/ml SWT for 6 hours.
| | Sample_growth_protocol_ch1 | The MCF-7 cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% fetal bovine serum (FBS), 1% non-essential amino acids, 100 unit/mL penicillin, 100 μg/mL streptomycin, 1 mM sodium pyruvate, and 2 mM L-glutamine in an atmosphere of 5% CO2 at 37 °C.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted using QIAGEN RNeasy Mini Kit, following the manufacturer’s protocol.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA samples were prepared according to the standard Affymetrix GeneChip protocol.
| | Sample_hyb_protocol | For each sample, 10 µg of biotinylated cRNA spiked with bioB, bioC, bioD and cre (Hybridization Control) was hybridized to a Human Genome U133 Plus 2.0 microarray for 16 hours at 45ºC.
| | Sample_scan_protocol | GeneChips were scanned with an Affymetrix GeneChip® Scanner 3000.
| | Sample_data_processing | The probeset-level expression data were summarized from probe-level data with Robust Multiarray Averaging method (RMA) by taking all 24 microarrays together.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Leming,,Shi
| | Sample_contact_email | leming.shi@gmail.com
| | Sample_contact_phone | 870-543-7387
| | Sample_contact_department | Systems Toxicology
| | Sample_contact_institute | NCTR/FDA
| | Sample_contact_address | 3900 NCTR Road
| | Sample_contact_city | Jefferson
| | Sample_contact_state | AR
| | Sample_contact_zip/postal_code | 72079
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM578nnn/GSM578942/suppl/GSM578942.CEL.gz
| | Sample_series_id | GSE23610
| | Sample_data_row_count | 54675
| | |
|
GSM578943 | GPL570 |
|
MCF-7_0.001%DMSO_rep3
|
Human breast cancer cell line MCF-7
|
cell line: breast cancer cell line MCF-7
treatment: DMSO treated
|
Gene expression profiling data from human breast cancer cell line MCF-7
|
| Sample_geo_accession | GSM578943
| | Sample_status | Public on May 18 2011
| | Sample_submission_date | Aug 13 2010
| | Sample_last_update_date | May 18 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The MCF-7 cells were incubated with hormone free medium and treated by 0.001% DMSO (control group), 0.1 µM 17 β-estradiol, 0.1, 1 or 10 µM of ferulic acid (equivalent to 0.0194, 0.194, and 1.94 mg/ml), 0.0256, 0.256, and 2.56 mg/ml SWT for 6 hours.
| | Sample_growth_protocol_ch1 | The MCF-7 cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% fetal bovine serum (FBS), 1% non-essential amino acids, 100 unit/mL penicillin, 100 μg/mL streptomycin, 1 mM sodium pyruvate, and 2 mM L-glutamine in an atmosphere of 5% CO2 at 37 °C.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted using QIAGEN RNeasy Mini Kit, following the manufacturer’s protocol.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA samples were prepared according to the standard Affymetrix GeneChip protocol.
| | Sample_hyb_protocol | For each sample, 10 µg of biotinylated cRNA spiked with bioB, bioC, bioD and cre (Hybridization Control) was hybridized to a Human Genome U133 Plus 2.0 microarray for 16 hours at 45ºC.
| | Sample_scan_protocol | GeneChips were scanned with an Affymetrix GeneChip® Scanner 3000.
| | Sample_data_processing | The probeset-level expression data were summarized from probe-level data with Robust Multiarray Averaging method (RMA) by taking all 24 microarrays together.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Leming,,Shi
| | Sample_contact_email | leming.shi@gmail.com
| | Sample_contact_phone | 870-543-7387
| | Sample_contact_department | Systems Toxicology
| | Sample_contact_institute | NCTR/FDA
| | Sample_contact_address | 3900 NCTR Road
| | Sample_contact_city | Jefferson
| | Sample_contact_state | AR
| | Sample_contact_zip/postal_code | 72079
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM578nnn/GSM578943/suppl/GSM578943.CEL.gz
| | Sample_series_id | GSE23610
| | Sample_data_row_count | 54675
| | |
|
GSM578944 | GPL570 |
|
MCF-7_Estradiol_0.1μM_rep1
|
Human breast cancer cell line MCF-7
|
cell line: breast cancer cell line MCF-7
treatment: Estradiol treated
|
Gene expression profiling data from human breast cancer cell line MCF-7
|
| Sample_geo_accession | GSM578944
| | Sample_status | Public on May 18 2011
| | Sample_submission_date | Aug 13 2010
| | Sample_last_update_date | May 18 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The MCF-7 cells were incubated with hormone free medium and treated by 0.001% DMSO (control group), 0.1 µM 17 β-estradiol, 0.1, 1 or 10 µM of ferulic acid (equivalent to 0.0194, 0.194, and 1.94 mg/ml), 0.0256, 0.256, and 2.56 mg/ml SWT for 6 hours.
| | Sample_growth_protocol_ch1 | The MCF-7 cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% fetal bovine serum (FBS), 1% non-essential amino acids, 100 unit/mL penicillin, 100 μg/mL streptomycin, 1 mM sodium pyruvate, and 2 mM L-glutamine in an atmosphere of 5% CO2 at 37 °C.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted using QIAGEN RNeasy Mini Kit, following the manufacturer’s protocol.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA samples were prepared according to the standard Affymetrix GeneChip protocol.
| | Sample_hyb_protocol | For each sample, 10 µg of biotinylated cRNA spiked with bioB, bioC, bioD and cre (Hybridization Control) was hybridized to a Human Genome U133 Plus 2.0 microarray for 16 hours at 45ºC.
| | Sample_scan_protocol | GeneChips were scanned with an Affymetrix GeneChip® Scanner 3000.
| | Sample_data_processing | The probeset-level expression data were summarized from probe-level data with Robust Multiarray Averaging method (RMA) by taking all 24 microarrays together.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Leming,,Shi
| | Sample_contact_email | leming.shi@gmail.com
| | Sample_contact_phone | 870-543-7387
| | Sample_contact_department | Systems Toxicology
| | Sample_contact_institute | NCTR/FDA
| | Sample_contact_address | 3900 NCTR Road
| | Sample_contact_city | Jefferson
| | Sample_contact_state | AR
| | Sample_contact_zip/postal_code | 72079
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM578nnn/GSM578944/suppl/GSM578944.CEL.gz
| | Sample_series_id | GSE23610
| | Sample_data_row_count | 54675
| | |
|
GSM578945 | GPL570 |
|
MCF-7_Estradiol_0.1μM_rep2
|
Human breast cancer cell line MCF-7
|
cell line: breast cancer cell line MCF-7
treatment: Estradiol treated
|
Gene expression profiling data from human breast cancer cell line MCF-7
|
| Sample_geo_accession | GSM578945
| | Sample_status | Public on May 18 2011
| | Sample_submission_date | Aug 13 2010
| | Sample_last_update_date | May 18 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The MCF-7 cells were incubated with hormone free medium and treated by 0.001% DMSO (control group), 0.1 µM 17 β-estradiol, 0.1, 1 or 10 µM of ferulic acid (equivalent to 0.0194, 0.194, and 1.94 mg/ml), 0.0256, 0.256, and 2.56 mg/ml SWT for 6 hours.
| | Sample_growth_protocol_ch1 | The MCF-7 cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% fetal bovine serum (FBS), 1% non-essential amino acids, 100 unit/mL penicillin, 100 μg/mL streptomycin, 1 mM sodium pyruvate, and 2 mM L-glutamine in an atmosphere of 5% CO2 at 37 °C.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted using QIAGEN RNeasy Mini Kit, following the manufacturer’s protocol.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA samples were prepared according to the standard Affymetrix GeneChip protocol.
| | Sample_hyb_protocol | For each sample, 10 µg of biotinylated cRNA spiked with bioB, bioC, bioD and cre (Hybridization Control) was hybridized to a Human Genome U133 Plus 2.0 microarray for 16 hours at 45ºC.
| | Sample_scan_protocol | GeneChips were scanned with an Affymetrix GeneChip® Scanner 3000.
| | Sample_data_processing | The probeset-level expression data were summarized from probe-level data with Robust Multiarray Averaging method (RMA) by taking all 24 microarrays together.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Leming,,Shi
| | Sample_contact_email | leming.shi@gmail.com
| | Sample_contact_phone | 870-543-7387
| | Sample_contact_department | Systems Toxicology
| | Sample_contact_institute | NCTR/FDA
| | Sample_contact_address | 3900 NCTR Road
| | Sample_contact_city | Jefferson
| | Sample_contact_state | AR
| | Sample_contact_zip/postal_code | 72079
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM578nnn/GSM578945/suppl/GSM578945.CEL.gz
| | Sample_series_id | GSE23610
| | Sample_data_row_count | 54675
| | |
|
GSM578946 | GPL570 |
|
MCF-7_Estradiol_0.1μM_rep3
|
Human breast cancer cell line MCF-7
|
cell line: breast cancer cell line MCF-7
treatment: Estradiol treated
|
Gene expression profiling data from human breast cancer cell line MCF-7
|
| Sample_geo_accession | GSM578946
| | Sample_status | Public on May 18 2011
| | Sample_submission_date | Aug 13 2010
| | Sample_last_update_date | May 18 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The MCF-7 cells were incubated with hormone free medium and treated by 0.001% DMSO (control group), 0.1 µM 17 β-estradiol, 0.1, 1 or 10 µM of ferulic acid (equivalent to 0.0194, 0.194, and 1.94 mg/ml), 0.0256, 0.256, and 2.56 mg/ml SWT for 6 hours.
| | Sample_growth_protocol_ch1 | The MCF-7 cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% fetal bovine serum (FBS), 1% non-essential amino acids, 100 unit/mL penicillin, 100 μg/mL streptomycin, 1 mM sodium pyruvate, and 2 mM L-glutamine in an atmosphere of 5% CO2 at 37 °C.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted using QIAGEN RNeasy Mini Kit, following the manufacturer’s protocol.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA samples were prepared according to the standard Affymetrix GeneChip protocol.
| | Sample_hyb_protocol | For each sample, 10 µg of biotinylated cRNA spiked with bioB, bioC, bioD and cre (Hybridization Control) was hybridized to a Human Genome U133 Plus 2.0 microarray for 16 hours at 45ºC.
| | Sample_scan_protocol | GeneChips were scanned with an Affymetrix GeneChip® Scanner 3000.
| | Sample_data_processing | The probeset-level expression data were summarized from probe-level data with Robust Multiarray Averaging method (RMA) by taking all 24 microarrays together.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Leming,,Shi
| | Sample_contact_email | leming.shi@gmail.com
| | Sample_contact_phone | 870-543-7387
| | Sample_contact_department | Systems Toxicology
| | Sample_contact_institute | NCTR/FDA
| | Sample_contact_address | 3900 NCTR Road
| | Sample_contact_city | Jefferson
| | Sample_contact_state | AR
| | Sample_contact_zip/postal_code | 72079
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM578nnn/GSM578946/suppl/GSM578946.CEL.gz
| | Sample_series_id | GSE23610
| | Sample_data_row_count | 54675
| | |
|
GSM578947 | GPL570 |
|
MCF-7_Ferulic acid_10μM_rep1
|
Human breast cancer cell line MCF-7
|
cell line: breast cancer cell line MCF-7
treatment: Ferulic acid treated
|
Gene expression profiling data from human breast cancer cell line MCF-7
|
| Sample_geo_accession | GSM578947
| | Sample_status | Public on May 18 2011
| | Sample_submission_date | Aug 13 2010
| | Sample_last_update_date | May 18 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The MCF-7 cells were incubated with hormone free medium and treated by 0.001% DMSO (control group), 0.1 µM 17 β-estradiol, 0.1, 1 or 10 µM of ferulic acid (equivalent to 0.0194, 0.194, and 1.94 mg/ml), 0.0256, 0.256, and 2.56 mg/ml SWT for 6 hours.
| | Sample_growth_protocol_ch1 | The MCF-7 cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% fetal bovine serum (FBS), 1% non-essential amino acids, 100 unit/mL penicillin, 100 μg/mL streptomycin, 1 mM sodium pyruvate, and 2 mM L-glutamine in an atmosphere of 5% CO2 at 37 °C.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted using QIAGEN RNeasy Mini Kit, following the manufacturer’s protocol.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA samples were prepared according to the standard Affymetrix GeneChip protocol.
| | Sample_hyb_protocol | For each sample, 10 µg of biotinylated cRNA spiked with bioB, bioC, bioD and cre (Hybridization Control) was hybridized to a Human Genome U133 Plus 2.0 microarray for 16 hours at 45ºC.
| | Sample_scan_protocol | GeneChips were scanned with an Affymetrix GeneChip® Scanner 3000.
| | Sample_data_processing | The probeset-level expression data were summarized from probe-level data with Robust Multiarray Averaging method (RMA) by taking all 24 microarrays together.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Leming,,Shi
| | Sample_contact_email | leming.shi@gmail.com
| | Sample_contact_phone | 870-543-7387
| | Sample_contact_department | Systems Toxicology
| | Sample_contact_institute | NCTR/FDA
| | Sample_contact_address | 3900 NCTR Road
| | Sample_contact_city | Jefferson
| | Sample_contact_state | AR
| | Sample_contact_zip/postal_code | 72079
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM578nnn/GSM578947/suppl/GSM578947.CEL.gz
| | Sample_series_id | GSE23610
| | Sample_data_row_count | 54675
| | |
|
GSM578948 | GPL570 |
|
MCF-7_Ferulic acid_10μM_rep2
|
Human breast cancer cell line MCF-7
|
cell line: breast cancer cell line MCF-7
treatment: Ferulic acid treated
|
Gene expression profiling data from human breast cancer cell line MCF-7
|
| Sample_geo_accession | GSM578948
| | Sample_status | Public on May 18 2011
| | Sample_submission_date | Aug 13 2010
| | Sample_last_update_date | May 18 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The MCF-7 cells were incubated with hormone free medium and treated by 0.001% DMSO (control group), 0.1 µM 17 β-estradiol, 0.1, 1 or 10 µM of ferulic acid (equivalent to 0.0194, 0.194, and 1.94 mg/ml), 0.0256, 0.256, and 2.56 mg/ml SWT for 6 hours.
| | Sample_growth_protocol_ch1 | The MCF-7 cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% fetal bovine serum (FBS), 1% non-essential amino acids, 100 unit/mL penicillin, 100 μg/mL streptomycin, 1 mM sodium pyruvate, and 2 mM L-glutamine in an atmosphere of 5% CO2 at 37 °C.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted using QIAGEN RNeasy Mini Kit, following the manufacturer’s protocol.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA samples were prepared according to the standard Affymetrix GeneChip protocol.
| | Sample_hyb_protocol | For each sample, 10 µg of biotinylated cRNA spiked with bioB, bioC, bioD and cre (Hybridization Control) was hybridized to a Human Genome U133 Plus 2.0 microarray for 16 hours at 45ºC.
| | Sample_scan_protocol | GeneChips were scanned with an Affymetrix GeneChip® Scanner 3000.
| | Sample_data_processing | The probeset-level expression data were summarized from probe-level data with Robust Multiarray Averaging method (RMA) by taking all 24 microarrays together.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Leming,,Shi
| | Sample_contact_email | leming.shi@gmail.com
| | Sample_contact_phone | 870-543-7387
| | Sample_contact_department | Systems Toxicology
| | Sample_contact_institute | NCTR/FDA
| | Sample_contact_address | 3900 NCTR Road
| | Sample_contact_city | Jefferson
| | Sample_contact_state | AR
| | Sample_contact_zip/postal_code | 72079
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM578nnn/GSM578948/suppl/GSM578948.CEL.gz
| | Sample_series_id | GSE23610
| | Sample_data_row_count | 54675
| | |
|
GSM578949 | GPL570 |
|
MCF-7_Ferulic acid_10μM_rep3
|
Human breast cancer cell line MCF-7
|
cell line: breast cancer cell line MCF-7
treatment: Ferulic acid treated
|
Gene expression profiling data from human breast cancer cell line MCF-7
|
| Sample_geo_accession | GSM578949
| | Sample_status | Public on May 18 2011
| | Sample_submission_date | Aug 13 2010
| | Sample_last_update_date | May 18 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The MCF-7 cells were incubated with hormone free medium and treated by 0.001% DMSO (control group), 0.1 µM 17 β-estradiol, 0.1, 1 or 10 µM of ferulic acid (equivalent to 0.0194, 0.194, and 1.94 mg/ml), 0.0256, 0.256, and 2.56 mg/ml SWT for 6 hours.
| | Sample_growth_protocol_ch1 | The MCF-7 cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% fetal bovine serum (FBS), 1% non-essential amino acids, 100 unit/mL penicillin, 100 μg/mL streptomycin, 1 mM sodium pyruvate, and 2 mM L-glutamine in an atmosphere of 5% CO2 at 37 °C.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted using QIAGEN RNeasy Mini Kit, following the manufacturer’s protocol.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA samples were prepared according to the standard Affymetrix GeneChip protocol.
| | Sample_hyb_protocol | For each sample, 10 µg of biotinylated cRNA spiked with bioB, bioC, bioD and cre (Hybridization Control) was hybridized to a Human Genome U133 Plus 2.0 microarray for 16 hours at 45ºC.
| | Sample_scan_protocol | GeneChips were scanned with an Affymetrix GeneChip® Scanner 3000.
| | Sample_data_processing | The probeset-level expression data were summarized from probe-level data with Robust Multiarray Averaging method (RMA) by taking all 24 microarrays together.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Leming,,Shi
| | Sample_contact_email | leming.shi@gmail.com
| | Sample_contact_phone | 870-543-7387
| | Sample_contact_department | Systems Toxicology
| | Sample_contact_institute | NCTR/FDA
| | Sample_contact_address | 3900 NCTR Road
| | Sample_contact_city | Jefferson
| | Sample_contact_state | AR
| | Sample_contact_zip/postal_code | 72079
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM578nnn/GSM578949/suppl/GSM578949.CEL.gz
| | Sample_series_id | GSE23610
| | Sample_data_row_count | 54675
| | |
|
GSM578950 | GPL570 |
|
MCF-7_Ferulic acid_0.1μM_rep1
|
Human breast cancer cell line MCF-7
|
cell line: breast cancer cell line MCF-7
treatment: Ferulic acid treated
|
Gene expression profiling data from human breast cancer cell line MCF-7
|
| Sample_geo_accession | GSM578950
| | Sample_status | Public on May 18 2011
| | Sample_submission_date | Aug 13 2010
| | Sample_last_update_date | May 18 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The MCF-7 cells were incubated with hormone free medium and treated by 0.001% DMSO (control group), 0.1 µM 17 β-estradiol, 0.1, 1 or 10 µM of ferulic acid (equivalent to 0.0194, 0.194, and 1.94 mg/ml), 0.0256, 0.256, and 2.56 mg/ml SWT for 6 hours.
| | Sample_growth_protocol_ch1 | The MCF-7 cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% fetal bovine serum (FBS), 1% non-essential amino acids, 100 unit/mL penicillin, 100 μg/mL streptomycin, 1 mM sodium pyruvate, and 2 mM L-glutamine in an atmosphere of 5% CO2 at 37 °C.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted using QIAGEN RNeasy Mini Kit, following the manufacturer’s protocol.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA samples were prepared according to the standard Affymetrix GeneChip protocol.
| | Sample_hyb_protocol | For each sample, 10 µg of biotinylated cRNA spiked with bioB, bioC, bioD and cre (Hybridization Control) was hybridized to a Human Genome U133 Plus 2.0 microarray for 16 hours at 45ºC.
| | Sample_scan_protocol | GeneChips were scanned with an Affymetrix GeneChip® Scanner 3000.
| | Sample_data_processing | The probeset-level expression data were summarized from probe-level data with Robust Multiarray Averaging method (RMA) by taking all 24 microarrays together.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Leming,,Shi
| | Sample_contact_email | leming.shi@gmail.com
| | Sample_contact_phone | 870-543-7387
| | Sample_contact_department | Systems Toxicology
| | Sample_contact_institute | NCTR/FDA
| | Sample_contact_address | 3900 NCTR Road
| | Sample_contact_city | Jefferson
| | Sample_contact_state | AR
| | Sample_contact_zip/postal_code | 72079
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM578nnn/GSM578950/suppl/GSM578950.CEL.gz
| | Sample_series_id | GSE23610
| | Sample_data_row_count | 54675
| | |
|
GSM578951 | GPL570 |
|
MCF-7_Ferulic acid_0.1μM_rep2
|
Human breast cancer cell line MCF-7
|
cell line: breast cancer cell line MCF-7
treatment: Ferulic acid treated
|
Gene expression profiling data from human breast cancer cell line MCF-7
|
| Sample_geo_accession | GSM578951
| | Sample_status | Public on May 18 2011
| | Sample_submission_date | Aug 13 2010
| | Sample_last_update_date | May 18 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The MCF-7 cells were incubated with hormone free medium and treated by 0.001% DMSO (control group), 0.1 µM 17 β-estradiol, 0.1, 1 or 10 µM of ferulic acid (equivalent to 0.0194, 0.194, and 1.94 mg/ml), 0.0256, 0.256, and 2.56 mg/ml SWT for 6 hours.
| | Sample_growth_protocol_ch1 | The MCF-7 cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% fetal bovine serum (FBS), 1% non-essential amino acids, 100 unit/mL penicillin, 100 μg/mL streptomycin, 1 mM sodium pyruvate, and 2 mM L-glutamine in an atmosphere of 5% CO2 at 37 °C.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted using QIAGEN RNeasy Mini Kit, following the manufacturer’s protocol.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA samples were prepared according to the standard Affymetrix GeneChip protocol.
| | Sample_hyb_protocol | For each sample, 10 µg of biotinylated cRNA spiked with bioB, bioC, bioD and cre (Hybridization Control) was hybridized to a Human Genome U133 Plus 2.0 microarray for 16 hours at 45ºC.
| | Sample_scan_protocol | GeneChips were scanned with an Affymetrix GeneChip® Scanner 3000.
| | Sample_data_processing | The probeset-level expression data were summarized from probe-level data with Robust Multiarray Averaging method (RMA) by taking all 24 microarrays together.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Leming,,Shi
| | Sample_contact_email | leming.shi@gmail.com
| | Sample_contact_phone | 870-543-7387
| | Sample_contact_department | Systems Toxicology
| | Sample_contact_institute | NCTR/FDA
| | Sample_contact_address | 3900 NCTR Road
| | Sample_contact_city | Jefferson
| | Sample_contact_state | AR
| | Sample_contact_zip/postal_code | 72079
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM578nnn/GSM578951/suppl/GSM578951.CEL.gz
| | Sample_series_id | GSE23610
| | Sample_data_row_count | 54675
| | |
|
GSM578952 | GPL570 |
|
MCF-7_Ferulic acid_0.1μM_rep3
|
Human breast cancer cell line MCF-7
|
cell line: breast cancer cell line MCF-7
treatment: Ferulic acid treated
|
Gene expression profiling data from human breast cancer cell line MCF-7
|
| Sample_geo_accession | GSM578952
| | Sample_status | Public on May 18 2011
| | Sample_submission_date | Aug 13 2010
| | Sample_last_update_date | May 18 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The MCF-7 cells were incubated with hormone free medium and treated by 0.001% DMSO (control group), 0.1 µM 17 β-estradiol, 0.1, 1 or 10 µM of ferulic acid (equivalent to 0.0194, 0.194, and 1.94 mg/ml), 0.0256, 0.256, and 2.56 mg/ml SWT for 6 hours.
| | Sample_growth_protocol_ch1 | The MCF-7 cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% fetal bovine serum (FBS), 1% non-essential amino acids, 100 unit/mL penicillin, 100 μg/mL streptomycin, 1 mM sodium pyruvate, and 2 mM L-glutamine in an atmosphere of 5% CO2 at 37 °C.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted using QIAGEN RNeasy Mini Kit, following the manufacturer’s protocol.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA samples were prepared according to the standard Affymetrix GeneChip protocol.
| | Sample_hyb_protocol | For each sample, 10 µg of biotinylated cRNA spiked with bioB, bioC, bioD and cre (Hybridization Control) was hybridized to a Human Genome U133 Plus 2.0 microarray for 16 hours at 45ºC.
| | Sample_scan_protocol | GeneChips were scanned with an Affymetrix GeneChip® Scanner 3000.
| | Sample_data_processing | The probeset-level expression data were summarized from probe-level data with Robust Multiarray Averaging method (RMA) by taking all 24 microarrays together.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Leming,,Shi
| | Sample_contact_email | leming.shi@gmail.com
| | Sample_contact_phone | 870-543-7387
| | Sample_contact_department | Systems Toxicology
| | Sample_contact_institute | NCTR/FDA
| | Sample_contact_address | 3900 NCTR Road
| | Sample_contact_city | Jefferson
| | Sample_contact_state | AR
| | Sample_contact_zip/postal_code | 72079
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM578nnn/GSM578952/suppl/GSM578952.CEL.gz
| | Sample_series_id | GSE23610
| | Sample_data_row_count | 54675
| | |
|
GSM578953 | GPL570 |
|
MCF-7_Ferulic acid_1μM_rep1
|
Human breast cancer cell line MCF-7
|
cell line: breast cancer cell line MCF-7
treatment: Ferulic acid treated
|
Gene expression profiling data from human breast cancer cell line MCF-7
|
| Sample_geo_accession | GSM578953
| | Sample_status | Public on May 18 2011
| | Sample_submission_date | Aug 13 2010
| | Sample_last_update_date | May 18 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The MCF-7 cells were incubated with hormone free medium and treated by 0.001% DMSO (control group), 0.1 µM 17 β-estradiol, 0.1, 1 or 10 µM of ferulic acid (equivalent to 0.0194, 0.194, and 1.94 mg/ml), 0.0256, 0.256, and 2.56 mg/ml SWT for 6 hours.
| | Sample_growth_protocol_ch1 | The MCF-7 cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% fetal bovine serum (FBS), 1% non-essential amino acids, 100 unit/mL penicillin, 100 μg/mL streptomycin, 1 mM sodium pyruvate, and 2 mM L-glutamine in an atmosphere of 5% CO2 at 37 °C.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted using QIAGEN RNeasy Mini Kit, following the manufacturer’s protocol.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA samples were prepared according to the standard Affymetrix GeneChip protocol.
| | Sample_hyb_protocol | For each sample, 10 µg of biotinylated cRNA spiked with bioB, bioC, bioD and cre (Hybridization Control) was hybridized to a Human Genome U133 Plus 2.0 microarray for 16 hours at 45ºC.
| | Sample_scan_protocol | GeneChips were scanned with an Affymetrix GeneChip® Scanner 3000.
| | Sample_data_processing | The probeset-level expression data were summarized from probe-level data with Robust Multiarray Averaging method (RMA) by taking all 24 microarrays together.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Leming,,Shi
| | Sample_contact_email | leming.shi@gmail.com
| | Sample_contact_phone | 870-543-7387
| | Sample_contact_department | Systems Toxicology
| | Sample_contact_institute | NCTR/FDA
| | Sample_contact_address | 3900 NCTR Road
| | Sample_contact_city | Jefferson
| | Sample_contact_state | AR
| | Sample_contact_zip/postal_code | 72079
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM578nnn/GSM578953/suppl/GSM578953.CEL.gz
| | Sample_series_id | GSE23610
| | Sample_data_row_count | 54675
| | |
|
GSM578954 | GPL570 |
|
MCF-7_Ferulic acid_1μM_rep2
|
Human breast cancer cell line MCF-7
|
cell line: breast cancer cell line MCF-7
treatment: Ferulic acid treated
|
Gene expression profiling data from human breast cancer cell line MCF-7
|
| Sample_geo_accession | GSM578954
| | Sample_status | Public on May 18 2011
| | Sample_submission_date | Aug 13 2010
| | Sample_last_update_date | May 18 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The MCF-7 cells were incubated with hormone free medium and treated by 0.001% DMSO (control group), 0.1 µM 17 β-estradiol, 0.1, 1 or 10 µM of ferulic acid (equivalent to 0.0194, 0.194, and 1.94 mg/ml), 0.0256, 0.256, and 2.56 mg/ml SWT for 6 hours.
| | Sample_growth_protocol_ch1 | The MCF-7 cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% fetal bovine serum (FBS), 1% non-essential amino acids, 100 unit/mL penicillin, 100 μg/mL streptomycin, 1 mM sodium pyruvate, and 2 mM L-glutamine in an atmosphere of 5% CO2 at 37 °C.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted using QIAGEN RNeasy Mini Kit, following the manufacturer’s protocol.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA samples were prepared according to the standard Affymetrix GeneChip protocol.
| | Sample_hyb_protocol | For each sample, 10 µg of biotinylated cRNA spiked with bioB, bioC, bioD and cre (Hybridization Control) was hybridized to a Human Genome U133 Plus 2.0 microarray for 16 hours at 45ºC.
| | Sample_scan_protocol | GeneChips were scanned with an Affymetrix GeneChip® Scanner 3000.
| | Sample_data_processing | The probeset-level expression data were summarized from probe-level data with Robust Multiarray Averaging method (RMA) by taking all 24 microarrays together.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Leming,,Shi
| | Sample_contact_email | leming.shi@gmail.com
| | Sample_contact_phone | 870-543-7387
| | Sample_contact_department | Systems Toxicology
| | Sample_contact_institute | NCTR/FDA
| | Sample_contact_address | 3900 NCTR Road
| | Sample_contact_city | Jefferson
| | Sample_contact_state | AR
| | Sample_contact_zip/postal_code | 72079
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM578nnn/GSM578954/suppl/GSM578954.CEL.gz
| | Sample_series_id | GSE23610
| | Sample_data_row_count | 54675
| | |
|
GSM578955 | GPL570 |
|
MCF-7_Ferulic acid_1μM_rep3
|
Human breast cancer cell line MCF-7
|
cell line: breast cancer cell line MCF-7
treatment: Ferulic acid treated
|
Gene expression profiling data from human breast cancer cell line MCF-7
|
| Sample_geo_accession | GSM578955
| | Sample_status | Public on May 18 2011
| | Sample_submission_date | Aug 13 2010
| | Sample_last_update_date | May 18 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The MCF-7 cells were incubated with hormone free medium and treated by 0.001% DMSO (control group), 0.1 µM 17 β-estradiol, 0.1, 1 or 10 µM of ferulic acid (equivalent to 0.0194, 0.194, and 1.94 mg/ml), 0.0256, 0.256, and 2.56 mg/ml SWT for 6 hours.
| | Sample_growth_protocol_ch1 | The MCF-7 cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% fetal bovine serum (FBS), 1% non-essential amino acids, 100 unit/mL penicillin, 100 μg/mL streptomycin, 1 mM sodium pyruvate, and 2 mM L-glutamine in an atmosphere of 5% CO2 at 37 °C.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted using QIAGEN RNeasy Mini Kit, following the manufacturer’s protocol.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA samples were prepared according to the standard Affymetrix GeneChip protocol.
| | Sample_hyb_protocol | For each sample, 10 µg of biotinylated cRNA spiked with bioB, bioC, bioD and cre (Hybridization Control) was hybridized to a Human Genome U133 Plus 2.0 microarray for 16 hours at 45ºC.
| | Sample_scan_protocol | GeneChips were scanned with an Affymetrix GeneChip® Scanner 3000.
| | Sample_data_processing | The probeset-level expression data were summarized from probe-level data with Robust Multiarray Averaging method (RMA) by taking all 24 microarrays together.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Leming,,Shi
| | Sample_contact_email | leming.shi@gmail.com
| | Sample_contact_phone | 870-543-7387
| | Sample_contact_department | Systems Toxicology
| | Sample_contact_institute | NCTR/FDA
| | Sample_contact_address | 3900 NCTR Road
| | Sample_contact_city | Jefferson
| | Sample_contact_state | AR
| | Sample_contact_zip/postal_code | 72079
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM578nnn/GSM578955/suppl/GSM578955.CEL.gz
| | Sample_series_id | GSE23610
| | Sample_data_row_count | 54675
| | |
|
GSM578956 | GPL570 |
|
MCF-7_Si-Wu-Tang_2.56mg/ml_rep1
|
Human breast cancer cell line MCF-7
|
cell line: breast cancer cell line MCF-7
treatment: Si-Wu-Tang treated
|
Gene expression profiling data from human breast cancer cell line MCF-7
|
| Sample_geo_accession | GSM578956
| | Sample_status | Public on May 18 2011
| | Sample_submission_date | Aug 13 2010
| | Sample_last_update_date | May 18 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The MCF-7 cells were incubated with hormone free medium and treated by 0.001% DMSO (control group), 0.1 µM 17 β-estradiol, 0.1, 1 or 10 µM of ferulic acid (equivalent to 0.0194, 0.194, and 1.94 mg/ml), 0.0256, 0.256, and 2.56 mg/ml SWT for 6 hours.
| | Sample_growth_protocol_ch1 | The MCF-7 cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% fetal bovine serum (FBS), 1% non-essential amino acids, 100 unit/mL penicillin, 100 μg/mL streptomycin, 1 mM sodium pyruvate, and 2 mM L-glutamine in an atmosphere of 5% CO2 at 37 °C.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted using QIAGEN RNeasy Mini Kit, following the manufacturer’s protocol.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA samples were prepared according to the standard Affymetrix GeneChip protocol.
| | Sample_hyb_protocol | For each sample, 10 µg of biotinylated cRNA spiked with bioB, bioC, bioD and cre (Hybridization Control) was hybridized to a Human Genome U133 Plus 2.0 microarray for 16 hours at 45ºC.
| | Sample_scan_protocol | GeneChips were scanned with an Affymetrix GeneChip® Scanner 3000.
| | Sample_data_processing | The probeset-level expression data were summarized from probe-level data with Robust Multiarray Averaging method (RMA) by taking all 24 microarrays together.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Leming,,Shi
| | Sample_contact_email | leming.shi@gmail.com
| | Sample_contact_phone | 870-543-7387
| | Sample_contact_department | Systems Toxicology
| | Sample_contact_institute | NCTR/FDA
| | Sample_contact_address | 3900 NCTR Road
| | Sample_contact_city | Jefferson
| | Sample_contact_state | AR
| | Sample_contact_zip/postal_code | 72079
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM578nnn/GSM578956/suppl/GSM578956.CEL.gz
| | Sample_series_id | GSE23610
| | Sample_data_row_count | 54675
| | |
|
GSM578957 | GPL570 |
|
MCF-7_Si-Wu-Tang_2.56mg/ml_rep2
|
Human breast cancer cell line MCF-7
|
cell line: breast cancer cell line MCF-7
treatment: Si-Wu-Tang treated
|
Gene expression profiling data from human breast cancer cell line MCF-7
|
| Sample_geo_accession | GSM578957
| | Sample_status | Public on May 18 2011
| | Sample_submission_date | Aug 13 2010
| | Sample_last_update_date | May 18 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The MCF-7 cells were incubated with hormone free medium and treated by 0.001% DMSO (control group), 0.1 µM 17 β-estradiol, 0.1, 1 or 10 µM of ferulic acid (equivalent to 0.0194, 0.194, and 1.94 mg/ml), 0.0256, 0.256, and 2.56 mg/ml SWT for 6 hours.
| | Sample_growth_protocol_ch1 | The MCF-7 cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% fetal bovine serum (FBS), 1% non-essential amino acids, 100 unit/mL penicillin, 100 μg/mL streptomycin, 1 mM sodium pyruvate, and 2 mM L-glutamine in an atmosphere of 5% CO2 at 37 °C.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted using QIAGEN RNeasy Mini Kit, following the manufacturer’s protocol.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA samples were prepared according to the standard Affymetrix GeneChip protocol.
| | Sample_hyb_protocol | For each sample, 10 µg of biotinylated cRNA spiked with bioB, bioC, bioD and cre (Hybridization Control) was hybridized to a Human Genome U133 Plus 2.0 microarray for 16 hours at 45ºC.
| | Sample_scan_protocol | GeneChips were scanned with an Affymetrix GeneChip® Scanner 3000.
| | Sample_data_processing | The probeset-level expression data were summarized from probe-level data with Robust Multiarray Averaging method (RMA) by taking all 24 microarrays together.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Leming,,Shi
| | Sample_contact_email | leming.shi@gmail.com
| | Sample_contact_phone | 870-543-7387
| | Sample_contact_department | Systems Toxicology
| | Sample_contact_institute | NCTR/FDA
| | Sample_contact_address | 3900 NCTR Road
| | Sample_contact_city | Jefferson
| | Sample_contact_state | AR
| | Sample_contact_zip/postal_code | 72079
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM578nnn/GSM578957/suppl/GSM578957.CEL.gz
| | Sample_series_id | GSE23610
| | Sample_data_row_count | 54675
| | |
|
GSM578958 | GPL570 |
|
MCF-7_Si-Wu-Tang_2.56mg/ml_rep3
|
Human breast cancer cell line MCF-7
|
cell line: breast cancer cell line MCF-7
treatment: Si-Wu-Tang treated
|
Gene expression profiling data from human breast cancer cell line MCF-7
|
| Sample_geo_accession | GSM578958
| | Sample_status | Public on May 18 2011
| | Sample_submission_date | Aug 13 2010
| | Sample_last_update_date | May 18 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The MCF-7 cells were incubated with hormone free medium and treated by 0.001% DMSO (control group), 0.1 µM 17 β-estradiol, 0.1, 1 or 10 µM of ferulic acid (equivalent to 0.0194, 0.194, and 1.94 mg/ml), 0.0256, 0.256, and 2.56 mg/ml SWT for 6 hours.
| | Sample_growth_protocol_ch1 | The MCF-7 cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% fetal bovine serum (FBS), 1% non-essential amino acids, 100 unit/mL penicillin, 100 μg/mL streptomycin, 1 mM sodium pyruvate, and 2 mM L-glutamine in an atmosphere of 5% CO2 at 37 °C.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted using QIAGEN RNeasy Mini Kit, following the manufacturer’s protocol.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA samples were prepared according to the standard Affymetrix GeneChip protocol.
| | Sample_hyb_protocol | For each sample, 10 µg of biotinylated cRNA spiked with bioB, bioC, bioD and cre (Hybridization Control) was hybridized to a Human Genome U133 Plus 2.0 microarray for 16 hours at 45ºC.
| | Sample_scan_protocol | GeneChips were scanned with an Affymetrix GeneChip® Scanner 3000.
| | Sample_data_processing | The probeset-level expression data were summarized from probe-level data with Robust Multiarray Averaging method (RMA) by taking all 24 microarrays together.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Leming,,Shi
| | Sample_contact_email | leming.shi@gmail.com
| | Sample_contact_phone | 870-543-7387
| | Sample_contact_department | Systems Toxicology
| | Sample_contact_institute | NCTR/FDA
| | Sample_contact_address | 3900 NCTR Road
| | Sample_contact_city | Jefferson
| | Sample_contact_state | AR
| | Sample_contact_zip/postal_code | 72079
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM578nnn/GSM578958/suppl/GSM578958.CEL.gz
| | Sample_series_id | GSE23610
| | Sample_data_row_count | 54675
| | |
|
GSM578959 | GPL570 |
|
MCF-7_Si-Wu-Tang_0.0256mg/ml_rep1
|
Human breast cancer cell line MCF-7
|
cell line: breast cancer cell line MCF-7
treatment: Si-Wu-Tang treated
|
Gene expression profiling data from human breast cancer cell line MCF-7
|
| Sample_geo_accession | GSM578959
| | Sample_status | Public on May 18 2011
| | Sample_submission_date | Aug 13 2010
| | Sample_last_update_date | May 18 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The MCF-7 cells were incubated with hormone free medium and treated by 0.001% DMSO (control group), 0.1 µM 17 β-estradiol, 0.1, 1 or 10 µM of ferulic acid (equivalent to 0.0194, 0.194, and 1.94 mg/ml), 0.0256, 0.256, and 2.56 mg/ml SWT for 6 hours.
| | Sample_growth_protocol_ch1 | The MCF-7 cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% fetal bovine serum (FBS), 1% non-essential amino acids, 100 unit/mL penicillin, 100 μg/mL streptomycin, 1 mM sodium pyruvate, and 2 mM L-glutamine in an atmosphere of 5% CO2 at 37 °C.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted using QIAGEN RNeasy Mini Kit, following the manufacturer’s protocol.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA samples were prepared according to the standard Affymetrix GeneChip protocol.
| | Sample_hyb_protocol | For each sample, 10 µg of biotinylated cRNA spiked with bioB, bioC, bioD and cre (Hybridization Control) was hybridized to a Human Genome U133 Plus 2.0 microarray for 16 hours at 45ºC.
| | Sample_scan_protocol | GeneChips were scanned with an Affymetrix GeneChip® Scanner 3000.
| | Sample_data_processing | The probeset-level expression data were summarized from probe-level data with Robust Multiarray Averaging method (RMA) by taking all 24 microarrays together.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Leming,,Shi
| | Sample_contact_email | leming.shi@gmail.com
| | Sample_contact_phone | 870-543-7387
| | Sample_contact_department | Systems Toxicology
| | Sample_contact_institute | NCTR/FDA
| | Sample_contact_address | 3900 NCTR Road
| | Sample_contact_city | Jefferson
| | Sample_contact_state | AR
| | Sample_contact_zip/postal_code | 72079
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM578nnn/GSM578959/suppl/GSM578959.CEL.gz
| | Sample_series_id | GSE23610
| | Sample_data_row_count | 54675
| | |
|
GSM578960 | GPL570 |
|
MCF-7_Si-Wu-Tang_0.0256mg/ml_rep2
|
Human breast cancer cell line MCF-7
|
cell line: breast cancer cell line MCF-7
treatment: Si-Wu-Tang treated
|
Gene expression profiling data from human breast cancer cell line MCF-7
|
| Sample_geo_accession | GSM578960
| | Sample_status | Public on May 18 2011
| | Sample_submission_date | Aug 13 2010
| | Sample_last_update_date | May 18 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The MCF-7 cells were incubated with hormone free medium and treated by 0.001% DMSO (control group), 0.1 µM 17 β-estradiol, 0.1, 1 or 10 µM of ferulic acid (equivalent to 0.0194, 0.194, and 1.94 mg/ml), 0.0256, 0.256, and 2.56 mg/ml SWT for 6 hours.
| | Sample_growth_protocol_ch1 | The MCF-7 cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% fetal bovine serum (FBS), 1% non-essential amino acids, 100 unit/mL penicillin, 100 μg/mL streptomycin, 1 mM sodium pyruvate, and 2 mM L-glutamine in an atmosphere of 5% CO2 at 37 °C.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted using QIAGEN RNeasy Mini Kit, following the manufacturer’s protocol.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA samples were prepared according to the standard Affymetrix GeneChip protocol.
| | Sample_hyb_protocol | For each sample, 10 µg of biotinylated cRNA spiked with bioB, bioC, bioD and cre (Hybridization Control) was hybridized to a Human Genome U133 Plus 2.0 microarray for 16 hours at 45ºC.
| | Sample_scan_protocol | GeneChips were scanned with an Affymetrix GeneChip® Scanner 3000.
| | Sample_data_processing | The probeset-level expression data were summarized from probe-level data with Robust Multiarray Averaging method (RMA) by taking all 24 microarrays together.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Leming,,Shi
| | Sample_contact_email | leming.shi@gmail.com
| | Sample_contact_phone | 870-543-7387
| | Sample_contact_department | Systems Toxicology
| | Sample_contact_institute | NCTR/FDA
| | Sample_contact_address | 3900 NCTR Road
| | Sample_contact_city | Jefferson
| | Sample_contact_state | AR
| | Sample_contact_zip/postal_code | 72079
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM578nnn/GSM578960/suppl/GSM578960.CEL.gz
| | Sample_series_id | GSE23610
| | Sample_data_row_count | 54675
| | |
|
GSM578961 | GPL570 |
|
MCF-7_Si-Wu-Tang_0.0256mg/ml_rep3
|
Human breast cancer cell line MCF-7
|
cell line: breast cancer cell line MCF-7
treatment: Si-Wu-Tang treated
|
Gene expression profiling data from human breast cancer cell line MCF-7
|
| Sample_geo_accession | GSM578961
| | Sample_status | Public on May 18 2011
| | Sample_submission_date | Aug 13 2010
| | Sample_last_update_date | May 18 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The MCF-7 cells were incubated with hormone free medium and treated by 0.001% DMSO (control group), 0.1 µM 17 β-estradiol, 0.1, 1 or 10 µM of ferulic acid (equivalent to 0.0194, 0.194, and 1.94 mg/ml), 0.0256, 0.256, and 2.56 mg/ml SWT for 6 hours.
| | Sample_growth_protocol_ch1 | The MCF-7 cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% fetal bovine serum (FBS), 1% non-essential amino acids, 100 unit/mL penicillin, 100 μg/mL streptomycin, 1 mM sodium pyruvate, and 2 mM L-glutamine in an atmosphere of 5% CO2 at 37 °C.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted using QIAGEN RNeasy Mini Kit, following the manufacturer’s protocol.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA samples were prepared according to the standard Affymetrix GeneChip protocol.
| | Sample_hyb_protocol | For each sample, 10 µg of biotinylated cRNA spiked with bioB, bioC, bioD and cre (Hybridization Control) was hybridized to a Human Genome U133 Plus 2.0 microarray for 16 hours at 45ºC.
| | Sample_scan_protocol | GeneChips were scanned with an Affymetrix GeneChip® Scanner 3000.
| | Sample_data_processing | The probeset-level expression data were summarized from probe-level data with Robust Multiarray Averaging method (RMA) by taking all 24 microarrays together.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Leming,,Shi
| | Sample_contact_email | leming.shi@gmail.com
| | Sample_contact_phone | 870-543-7387
| | Sample_contact_department | Systems Toxicology
| | Sample_contact_institute | NCTR/FDA
| | Sample_contact_address | 3900 NCTR Road
| | Sample_contact_city | Jefferson
| | Sample_contact_state | AR
| | Sample_contact_zip/postal_code | 72079
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM578nnn/GSM578961/suppl/GSM578961.CEL.gz
| | Sample_series_id | GSE23610
| | Sample_data_row_count | 54675
| | |
|
GSM578962 | GPL570 |
|
MCF-7_Si-Wu-Tang_0.256mg/ml_rep1
|
Human breast cancer cell line MCF-7
|
cell line: breast cancer cell line MCF-7
treatment: Si-Wu-Tang treated
|
Gene expression profiling data from human breast cancer cell line MCF-7
|
| Sample_geo_accession | GSM578962
| | Sample_status | Public on May 18 2011
| | Sample_submission_date | Aug 13 2010
| | Sample_last_update_date | May 18 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The MCF-7 cells were incubated with hormone free medium and treated by 0.001% DMSO (control group), 0.1 µM 17 β-estradiol, 0.1, 1 or 10 µM of ferulic acid (equivalent to 0.0194, 0.194, and 1.94 mg/ml), 0.0256, 0.256, and 2.56 mg/ml SWT for 6 hours.
| | Sample_growth_protocol_ch1 | The MCF-7 cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% fetal bovine serum (FBS), 1% non-essential amino acids, 100 unit/mL penicillin, 100 μg/mL streptomycin, 1 mM sodium pyruvate, and 2 mM L-glutamine in an atmosphere of 5% CO2 at 37 °C.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted using QIAGEN RNeasy Mini Kit, following the manufacturer’s protocol.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA samples were prepared according to the standard Affymetrix GeneChip protocol.
| | Sample_hyb_protocol | For each sample, 10 µg of biotinylated cRNA spiked with bioB, bioC, bioD and cre (Hybridization Control) was hybridized to a Human Genome U133 Plus 2.0 microarray for 16 hours at 45ºC.
| | Sample_scan_protocol | GeneChips were scanned with an Affymetrix GeneChip® Scanner 3000.
| | Sample_data_processing | The probeset-level expression data were summarized from probe-level data with Robust Multiarray Averaging method (RMA) by taking all 24 microarrays together.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Leming,,Shi
| | Sample_contact_email | leming.shi@gmail.com
| | Sample_contact_phone | 870-543-7387
| | Sample_contact_department | Systems Toxicology
| | Sample_contact_institute | NCTR/FDA
| | Sample_contact_address | 3900 NCTR Road
| | Sample_contact_city | Jefferson
| | Sample_contact_state | AR
| | Sample_contact_zip/postal_code | 72079
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM578nnn/GSM578962/suppl/GSM578962.CEL.gz
| | Sample_series_id | GSE23610
| | Sample_data_row_count | 54675
| | |
|
GSM578963 | GPL570 |
|
MCF-7_Si-Wu-Tang_0.256mg/ml_rep2
|
Human breast cancer cell line MCF-7
|
cell line: breast cancer cell line MCF-7
treatment: Si-Wu-Tang treated
|
Gene expression profiling data from human breast cancer cell line MCF-7
|
| Sample_geo_accession | GSM578963
| | Sample_status | Public on May 18 2011
| | Sample_submission_date | Aug 13 2010
| | Sample_last_update_date | May 18 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The MCF-7 cells were incubated with hormone free medium and treated by 0.001% DMSO (control group), 0.1 µM 17 β-estradiol, 0.1, 1 or 10 µM of ferulic acid (equivalent to 0.0194, 0.194, and 1.94 mg/ml), 0.0256, 0.256, and 2.56 mg/ml SWT for 6 hours.
| | Sample_growth_protocol_ch1 | The MCF-7 cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% fetal bovine serum (FBS), 1% non-essential amino acids, 100 unit/mL penicillin, 100 μg/mL streptomycin, 1 mM sodium pyruvate, and 2 mM L-glutamine in an atmosphere of 5% CO2 at 37 °C.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted using QIAGEN RNeasy Mini Kit, following the manufacturer’s protocol.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA samples were prepared according to the standard Affymetrix GeneChip protocol.
| | Sample_hyb_protocol | For each sample, 10 µg of biotinylated cRNA spiked with bioB, bioC, bioD and cre (Hybridization Control) was hybridized to a Human Genome U133 Plus 2.0 microarray for 16 hours at 45ºC.
| | Sample_scan_protocol | GeneChips were scanned with an Affymetrix GeneChip® Scanner 3000.
| | Sample_data_processing | The probeset-level expression data were summarized from probe-level data with Robust Multiarray Averaging method (RMA) by taking all 24 microarrays together.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Leming,,Shi
| | Sample_contact_email | leming.shi@gmail.com
| | Sample_contact_phone | 870-543-7387
| | Sample_contact_department | Systems Toxicology
| | Sample_contact_institute | NCTR/FDA
| | Sample_contact_address | 3900 NCTR Road
| | Sample_contact_city | Jefferson
| | Sample_contact_state | AR
| | Sample_contact_zip/postal_code | 72079
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM578nnn/GSM578963/suppl/GSM578963.CEL.gz
| | Sample_series_id | GSE23610
| | Sample_data_row_count | 54675
| | |
|
GSM578964 | GPL570 |
|
MCF-7_Si-Wu-Tang_0.256mg/ml_rep3
|
Human breast cancer cell line MCF-7
|
cell line: breast cancer cell line MCF-7
treatment: Si-Wu-Tang treated
|
Gene expression profiling data from human breast cancer cell line MCF-7
|
| Sample_geo_accession | GSM578964
| | Sample_status | Public on May 18 2011
| | Sample_submission_date | Aug 13 2010
| | Sample_last_update_date | May 18 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | The MCF-7 cells were incubated with hormone free medium and treated by 0.001% DMSO (control group), 0.1 µM 17 β-estradiol, 0.1, 1 or 10 µM of ferulic acid (equivalent to 0.0194, 0.194, and 1.94 mg/ml), 0.0256, 0.256, and 2.56 mg/ml SWT for 6 hours.
| | Sample_growth_protocol_ch1 | The MCF-7 cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% fetal bovine serum (FBS), 1% non-essential amino acids, 100 unit/mL penicillin, 100 μg/mL streptomycin, 1 mM sodium pyruvate, and 2 mM L-glutamine in an atmosphere of 5% CO2 at 37 °C.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted using QIAGEN RNeasy Mini Kit, following the manufacturer’s protocol.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA samples were prepared according to the standard Affymetrix GeneChip protocol.
| | Sample_hyb_protocol | For each sample, 10 µg of biotinylated cRNA spiked with bioB, bioC, bioD and cre (Hybridization Control) was hybridized to a Human Genome U133 Plus 2.0 microarray for 16 hours at 45ºC.
| | Sample_scan_protocol | GeneChips were scanned with an Affymetrix GeneChip® Scanner 3000.
| | Sample_data_processing | The probeset-level expression data were summarized from probe-level data with Robust Multiarray Averaging method (RMA) by taking all 24 microarrays together.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Leming,,Shi
| | Sample_contact_email | leming.shi@gmail.com
| | Sample_contact_phone | 870-543-7387
| | Sample_contact_department | Systems Toxicology
| | Sample_contact_institute | NCTR/FDA
| | Sample_contact_address | 3900 NCTR Road
| | Sample_contact_city | Jefferson
| | Sample_contact_state | AR
| | Sample_contact_zip/postal_code | 72079
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM578nnn/GSM578964/suppl/GSM578964.CEL.gz
| | Sample_series_id | GSE23610
| | Sample_data_row_count | 54675
| | |
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(2 groups will be compared at a time) |
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