Search results for the GEO ID: GSE23640 |
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(Once the selection is made, click on "Add groups" in "Make groups for comparison", to make a group. Scroll down) |
|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM579966 | GPL570 |
|
Breast cancer cell line HCC1396
|
Cell line
|
cell type: Breast cancer cell line
genotype: wt
invasive tumour type: Ductal
invasive b-r grade: III
er status: neg
pr status: neg
her2 status: neg
|
Gene expression data from breast cancer tumor cell line
|
Sample_geo_accession | GSM579966
| Sample_status | Public on Aug 17 2010
| Sample_submission_date | Aug 16 2010
| Sample_last_update_date | Aug 16 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Primary tumor samples were collagenase digested then sorted for epithelial cells using BerEp4 antibody coated magnetic beads
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Isolation of RNA was performed using the Trizol method (Invitrogen) according to the manufacturers instructions and was purified using RNeasy mini-columns (Qiagen, Valencia, CA). The quality of the RNA obtained from each tumour sample was assessed based on the RNA profile generated by the bioanalyzer (Agilent Inc)olation of RNA was performed using the Trizol method (Invitrogen) according to the manufacturers instructions and was purified using RNeasy mini-columns (Qiagen, Valencia, CA). The quality of the RNA obtained from each tumour sample was assessed based on the RNA profile generated by the bioanalyzer (Agilent Inc).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Approximately 1 microgram of total RNA was processed to produce biotinylated cRNA targets.
| Sample_hyb_protocol | standard Affymetrix procedures.
| Sample_scan_protocol | standard Affymetrix procedures.
| Sample_data_processing | Raw gene expression values were obtained using Affymetrix GeneChip software and further analyzed using the DNA-Chip Analyzer (dChip).
| Sample_platform_id | GPL570
| Sample_contact_name | Qiyuan,,Li
| Sample_contact_email | qiyuan@dfci.harvard.edu
| Sample_contact_phone | 617-582-8682
| Sample_contact_department | Medical Oncology
| Sample_contact_institute | Dana Farber Cancer Institute
| Sample_contact_address | 44 Binney street
| Sample_contact_city | Boston
| Sample_contact_state | MA
| Sample_contact_zip/postal_code | 02115
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM579nnn/GSM579966/suppl/GSM579966.CEL.gz
| Sample_series_id | GSE23640
| Sample_data_row_count | 54675
| |
|
GSM579970 | GPL570 |
|
Breast cancer cell line HCC1400
|
Cell line
|
cell type: Breast cancer cell line
genotype: wt
invasive tumour type: Ductal
invasive b-r grade: III
er status: neg
pr status: pos
her2 status: pos
|
Gene expression data from breast cancer tumor cell line
|
Sample_geo_accession | GSM579970
| Sample_status | Public on Aug 17 2010
| Sample_submission_date | Aug 16 2010
| Sample_last_update_date | Aug 16 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Primary tumor samples were collagenase digested then sorted for epithelial cells using BerEp4 antibody coated magnetic beads
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Isolation of RNA was performed using the Trizol method (Invitrogen) according to the manufacturers instructions and was purified using RNeasy mini-columns (Qiagen, Valencia, CA). The quality of the RNA obtained from each tumour sample was assessed based on the RNA profile generated by the bioanalyzer (Agilent Inc)olation of RNA was performed using the Trizol method (Invitrogen) according to the manufacturers instructions and was purified using RNeasy mini-columns (Qiagen, Valencia, CA). The quality of the RNA obtained from each tumour sample was assessed based on the RNA profile generated by the bioanalyzer (Agilent Inc).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Approximately 1 microgram of total RNA was processed to produce biotinylated cRNA targets.
| Sample_hyb_protocol | standard Affymetrix procedures.
| Sample_scan_protocol | standard Affymetrix procedures.
| Sample_data_processing | Raw gene expression values were obtained using Affymetrix GeneChip software and further analyzed using the DNA-Chip Analyzer (dChip).
| Sample_platform_id | GPL570
| Sample_contact_name | Qiyuan,,Li
| Sample_contact_email | qiyuan@dfci.harvard.edu
| Sample_contact_phone | 617-582-8682
| Sample_contact_department | Medical Oncology
| Sample_contact_institute | Dana Farber Cancer Institute
| Sample_contact_address | 44 Binney street
| Sample_contact_city | Boston
| Sample_contact_state | MA
| Sample_contact_zip/postal_code | 02115
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM579nnn/GSM579970/suppl/GSM579970.CEL.gz
| Sample_series_id | GSE23640
| Sample_data_row_count | 54675
| |
|
GSM579971 | GPL570 |
|
Breast cancer cell line HCC1401
|
Cell line
|
cell type: Breast cancer cell line
genotype: B1germ
invasive tumour type: Ductal
invasive b-r grade: III
er status: neg
pr status: neg
her2 status: neg
|
Gene expression data from breast cancer tumor cell line
|
Sample_geo_accession | GSM579971
| Sample_status | Public on Aug 17 2010
| Sample_submission_date | Aug 16 2010
| Sample_last_update_date | Aug 16 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Primary tumor samples were collagenase digested then sorted for epithelial cells using BerEp4 antibody coated magnetic beads
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Isolation of RNA was performed using the Trizol method (Invitrogen) according to the manufacturers instructions and was purified using RNeasy mini-columns (Qiagen, Valencia, CA). The quality of the RNA obtained from each tumour sample was assessed based on the RNA profile generated by the bioanalyzer (Agilent Inc)olation of RNA was performed using the Trizol method (Invitrogen) according to the manufacturers instructions and was purified using RNeasy mini-columns (Qiagen, Valencia, CA). The quality of the RNA obtained from each tumour sample was assessed based on the RNA profile generated by the bioanalyzer (Agilent Inc).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Approximately 1 microgram of total RNA was processed to produce biotinylated cRNA targets.
| Sample_hyb_protocol | standard Affymetrix procedures.
| Sample_scan_protocol | standard Affymetrix procedures.
| Sample_data_processing | Raw gene expression values were obtained using Affymetrix GeneChip software and further analyzed using the DNA-Chip Analyzer (dChip).
| Sample_platform_id | GPL570
| Sample_contact_name | Qiyuan,,Li
| Sample_contact_email | qiyuan@dfci.harvard.edu
| Sample_contact_phone | 617-582-8682
| Sample_contact_department | Medical Oncology
| Sample_contact_institute | Dana Farber Cancer Institute
| Sample_contact_address | 44 Binney street
| Sample_contact_city | Boston
| Sample_contact_state | MA
| Sample_contact_zip/postal_code | 02115
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM579nnn/GSM579971/suppl/GSM579971.CEL.gz
| Sample_series_id | GSE23640
| Sample_data_row_count | 54675
| |
|
GSM579972 | GPL570 |
|
Breast cancer cell line HCC1402
|
Cell line
|
cell type: Breast cancer cell line
genotype: B2som
invasive tumour type: Ductal
invasive b-r grade: III
er status: neg
pr status: neg
her2 status: neg
|
Gene expression data from breast cancer tumor cell line
|
Sample_geo_accession | GSM579972
| Sample_status | Public on Aug 17 2010
| Sample_submission_date | Aug 16 2010
| Sample_last_update_date | Aug 16 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Primary tumor samples were collagenase digested then sorted for epithelial cells using BerEp4 antibody coated magnetic beads
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Isolation of RNA was performed using the Trizol method (Invitrogen) according to the manufacturers instructions and was purified using RNeasy mini-columns (Qiagen, Valencia, CA). The quality of the RNA obtained from each tumour sample was assessed based on the RNA profile generated by the bioanalyzer (Agilent Inc)olation of RNA was performed using the Trizol method (Invitrogen) according to the manufacturers instructions and was purified using RNeasy mini-columns (Qiagen, Valencia, CA). The quality of the RNA obtained from each tumour sample was assessed based on the RNA profile generated by the bioanalyzer (Agilent Inc).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Approximately 1 microgram of total RNA was processed to produce biotinylated cRNA targets.
| Sample_hyb_protocol | standard Affymetrix procedures.
| Sample_scan_protocol | standard Affymetrix procedures.
| Sample_data_processing | Raw gene expression values were obtained using Affymetrix GeneChip software and further analyzed using the DNA-Chip Analyzer (dChip).
| Sample_platform_id | GPL570
| Sample_contact_name | Qiyuan,,Li
| Sample_contact_email | qiyuan@dfci.harvard.edu
| Sample_contact_phone | 617-582-8682
| Sample_contact_department | Medical Oncology
| Sample_contact_institute | Dana Farber Cancer Institute
| Sample_contact_address | 44 Binney street
| Sample_contact_city | Boston
| Sample_contact_state | MA
| Sample_contact_zip/postal_code | 02115
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM579nnn/GSM579972/suppl/GSM579972.CEL.gz
| Sample_series_id | GSE23640
| Sample_data_row_count | 54675
| |
|
GSM579973 | GPL570 |
|
Breast cancer cell line HCC1403
|
Cell line
|
cell type: Breast cancer cell line
genotype: wt
invasive tumour type: Unknown
invasive b-r grade: Unknown
er status: pos
pr status: pos
her2 status: neg
|
Gene expression data from breast cancer tumor cell line
|
Sample_geo_accession | GSM579973
| Sample_status | Public on Aug 17 2010
| Sample_submission_date | Aug 16 2010
| Sample_last_update_date | Aug 16 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Primary tumor samples were collagenase digested then sorted for epithelial cells using BerEp4 antibody coated magnetic beads
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Isolation of RNA was performed using the Trizol method (Invitrogen) according to the manufacturers instructions and was purified using RNeasy mini-columns (Qiagen, Valencia, CA). The quality of the RNA obtained from each tumour sample was assessed based on the RNA profile generated by the bioanalyzer (Agilent Inc)olation of RNA was performed using the Trizol method (Invitrogen) according to the manufacturers instructions and was purified using RNeasy mini-columns (Qiagen, Valencia, CA). The quality of the RNA obtained from each tumour sample was assessed based on the RNA profile generated by the bioanalyzer (Agilent Inc).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Approximately 1 microgram of total RNA was processed to produce biotinylated cRNA targets.
| Sample_hyb_protocol | standard Affymetrix procedures.
| Sample_scan_protocol | standard Affymetrix procedures.
| Sample_data_processing | Raw gene expression values were obtained using Affymetrix GeneChip software and further analyzed using the DNA-Chip Analyzer (dChip).
| Sample_platform_id | GPL570
| Sample_contact_name | Qiyuan,,Li
| Sample_contact_email | qiyuan@dfci.harvard.edu
| Sample_contact_phone | 617-582-8682
| Sample_contact_department | Medical Oncology
| Sample_contact_institute | Dana Farber Cancer Institute
| Sample_contact_address | 44 Binney street
| Sample_contact_city | Boston
| Sample_contact_state | MA
| Sample_contact_zip/postal_code | 02115
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM579nnn/GSM579973/suppl/GSM579973.CEL.gz
| Sample_series_id | GSE23640
| Sample_data_row_count | 54675
| |
|
GSM579974 | GPL570 |
|
sorted tumor epithelium 718
|
sorted tumor epithelium
|
cell type: sorted breast tumor epithelium
genotype: Unknown
invasive tumour type: Ductal
invasive b-r grade: I
er status: pos
pr status: pos
her2 status: neg
|
Gene expression data from breast cancer epithelial cells prified from tumor tissue using BerEp4 antibody coated magnetic beads.
|
Sample_geo_accession | GSM579974
| Sample_status | Public on Aug 17 2010
| Sample_submission_date | Aug 16 2010
| Sample_last_update_date | Aug 16 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Primary tumor samples were collagenase digested then sorted for epithelial cells using BerEp4 antibody coated magnetic beads
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Isolation of RNA was performed using the Trizol method (Invitrogen) according to the manufacturers instructions and was purified using RNeasy mini-columns (Qiagen, Valencia, CA). The quality of the RNA obtained from each tumour sample was assessed based on the RNA profile generated by the bioanalyzer (Agilent Inc)olation of RNA was performed using the Trizol method (Invitrogen) according to the manufacturers instructions and was purified using RNeasy mini-columns (Qiagen, Valencia, CA). The quality of the RNA obtained from each tumour sample was assessed based on the RNA profile generated by the bioanalyzer (Agilent Inc).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Approximately 1 microgram of total RNA was processed to produce biotinylated cRNA targets.
| Sample_hyb_protocol | standard Affymetrix procedures.
| Sample_scan_protocol | standard Affymetrix procedures.
| Sample_data_processing | Raw gene expression values were obtained using Affymetrix GeneChip software and further analyzed using the DNA-Chip Analyzer (dChip).
| Sample_platform_id | GPL570
| Sample_contact_name | Qiyuan,,Li
| Sample_contact_email | qiyuan@dfci.harvard.edu
| Sample_contact_phone | 617-582-8682
| Sample_contact_department | Medical Oncology
| Sample_contact_institute | Dana Farber Cancer Institute
| Sample_contact_address | 44 Binney street
| Sample_contact_city | Boston
| Sample_contact_state | MA
| Sample_contact_zip/postal_code | 02115
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM579nnn/GSM579974/suppl/GSM579974.CEL.gz
| Sample_series_id | GSE23640
| Sample_data_row_count | 54675
| |
|
GSM579975 | GPL570 |
|
sorted tumor epithelium 795
|
sorted tumor epithelium
|
cell type: sorted breast tumor epithelium
genotype: Unknown
invasive tumour type: Ductal
invasive b-r grade: III
er status: pos
pr status: pos
her2 status: neg
|
Gene expression data from breast cancer epithelial cells prified from tumor tissue using BerEp4 antibody coated magnetic beads.
|
Sample_geo_accession | GSM579975
| Sample_status | Public on Aug 17 2010
| Sample_submission_date | Aug 16 2010
| Sample_last_update_date | Aug 16 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Primary tumor samples were collagenase digested then sorted for epithelial cells using BerEp4 antibody coated magnetic beads
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Isolation of RNA was performed using the Trizol method (Invitrogen) according to the manufacturers instructions and was purified using RNeasy mini-columns (Qiagen, Valencia, CA). The quality of the RNA obtained from each tumour sample was assessed based on the RNA profile generated by the bioanalyzer (Agilent Inc)olation of RNA was performed using the Trizol method (Invitrogen) according to the manufacturers instructions and was purified using RNeasy mini-columns (Qiagen, Valencia, CA). The quality of the RNA obtained from each tumour sample was assessed based on the RNA profile generated by the bioanalyzer (Agilent Inc).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Approximately 1 microgram of total RNA was processed to produce biotinylated cRNA targets.
| Sample_hyb_protocol | standard Affymetrix procedures.
| Sample_scan_protocol | standard Affymetrix procedures.
| Sample_data_processing | Raw gene expression values were obtained using Affymetrix GeneChip software and further analyzed using the DNA-Chip Analyzer (dChip).
| Sample_platform_id | GPL570
| Sample_contact_name | Qiyuan,,Li
| Sample_contact_email | qiyuan@dfci.harvard.edu
| Sample_contact_phone | 617-582-8682
| Sample_contact_department | Medical Oncology
| Sample_contact_institute | Dana Farber Cancer Institute
| Sample_contact_address | 44 Binney street
| Sample_contact_city | Boston
| Sample_contact_state | MA
| Sample_contact_zip/postal_code | 02115
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM579nnn/GSM579975/suppl/GSM579975.CEL.gz
| Sample_series_id | GSE23640
| Sample_data_row_count | 54675
| |
|
GSM579976 | GPL570 |
|
sorted tumor epithelium 850
|
sorted tumor epithelium
|
cell type: sorted breast tumor epithelium
genotype: Unknown
invasive tumour type: M
invasive b-r grade: II
er status: pos
pr status: pos
her2 status: neg
|
Gene expression data from breast cancer epithelial cells prified from tumor tissue using BerEp4 antibody coated magnetic beads.
|
Sample_geo_accession | GSM579976
| Sample_status | Public on Aug 17 2010
| Sample_submission_date | Aug 16 2010
| Sample_last_update_date | Aug 16 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Primary tumor samples were collagenase digested then sorted for epithelial cells using BerEp4 antibody coated magnetic beads
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Isolation of RNA was performed using the Trizol method (Invitrogen) according to the manufacturers instructions and was purified using RNeasy mini-columns (Qiagen, Valencia, CA). The quality of the RNA obtained from each tumour sample was assessed based on the RNA profile generated by the bioanalyzer (Agilent Inc)olation of RNA was performed using the Trizol method (Invitrogen) according to the manufacturers instructions and was purified using RNeasy mini-columns (Qiagen, Valencia, CA). The quality of the RNA obtained from each tumour sample was assessed based on the RNA profile generated by the bioanalyzer (Agilent Inc).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Approximately 1 microgram of total RNA was processed to produce biotinylated cRNA targets.
| Sample_hyb_protocol | standard Affymetrix procedures.
| Sample_scan_protocol | standard Affymetrix procedures.
| Sample_data_processing | Raw gene expression values were obtained using Affymetrix GeneChip software and further analyzed using the DNA-Chip Analyzer (dChip).
| Sample_platform_id | GPL570
| Sample_contact_name | Qiyuan,,Li
| Sample_contact_email | qiyuan@dfci.harvard.edu
| Sample_contact_phone | 617-582-8682
| Sample_contact_department | Medical Oncology
| Sample_contact_institute | Dana Farber Cancer Institute
| Sample_contact_address | 44 Binney street
| Sample_contact_city | Boston
| Sample_contact_state | MA
| Sample_contact_zip/postal_code | 02115
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM579nnn/GSM579976/suppl/GSM579976.CEL.gz
| Sample_series_id | GSE23640
| Sample_data_row_count | 54675
| |
|
GSM579977 | GPL570 |
|
sorted tumor epithelium 818
|
sorted tumor epithelium
|
cell type: sorted breast tumor epithelium
genotype: Unknown
invasive tumour type: Ductal
invasive b-r grade: II
er status: pos
pr status: neg
her2 status: neg
|
Gene expression data from breast cancer epithelial cells prified from tumor tissue using BerEp4 antibody coated magnetic beads.
|
Sample_geo_accession | GSM579977
| Sample_status | Public on Aug 17 2010
| Sample_submission_date | Aug 16 2010
| Sample_last_update_date | Aug 16 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Primary tumor samples were collagenase digested then sorted for epithelial cells using BerEp4 antibody coated magnetic beads
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Isolation of RNA was performed using the Trizol method (Invitrogen) according to the manufacturers instructions and was purified using RNeasy mini-columns (Qiagen, Valencia, CA). The quality of the RNA obtained from each tumour sample was assessed based on the RNA profile generated by the bioanalyzer (Agilent Inc)olation of RNA was performed using the Trizol method (Invitrogen) according to the manufacturers instructions and was purified using RNeasy mini-columns (Qiagen, Valencia, CA). The quality of the RNA obtained from each tumour sample was assessed based on the RNA profile generated by the bioanalyzer (Agilent Inc).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Approximately 1 microgram of total RNA was processed to produce biotinylated cRNA targets.
| Sample_hyb_protocol | standard Affymetrix procedures.
| Sample_scan_protocol | standard Affymetrix procedures.
| Sample_data_processing | Raw gene expression values were obtained using Affymetrix GeneChip software and further analyzed using the DNA-Chip Analyzer (dChip).
| Sample_platform_id | GPL570
| Sample_contact_name | Qiyuan,,Li
| Sample_contact_email | qiyuan@dfci.harvard.edu
| Sample_contact_phone | 617-582-8682
| Sample_contact_department | Medical Oncology
| Sample_contact_institute | Dana Farber Cancer Institute
| Sample_contact_address | 44 Binney street
| Sample_contact_city | Boston
| Sample_contact_state | MA
| Sample_contact_zip/postal_code | 02115
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM579nnn/GSM579977/suppl/GSM579977.CEL.gz
| Sample_series_id | GSE23640
| Sample_data_row_count | 54675
| |
|
GSM579978 | GPL570 |
|
sorted tumor epithelium 838
|
sorted tumor epithelium
|
cell type: sorted breast tumor epithelium
genotype: Unknown
invasive tumour type: Ductal
invasive b-r grade: I
er status: pos
pr status: pos
her2 status: neg
|
Gene expression data from breast cancer epithelial cells prified from tumor tissue using BerEp4 antibody coated magnetic beads.
|
Sample_geo_accession | GSM579978
| Sample_status | Public on Aug 17 2010
| Sample_submission_date | Aug 16 2010
| Sample_last_update_date | Aug 16 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Primary tumor samples were collagenase digested then sorted for epithelial cells using BerEp4 antibody coated magnetic beads
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Isolation of RNA was performed using the Trizol method (Invitrogen) according to the manufacturers instructions and was purified using RNeasy mini-columns (Qiagen, Valencia, CA). The quality of the RNA obtained from each tumour sample was assessed based on the RNA profile generated by the bioanalyzer (Agilent Inc)olation of RNA was performed using the Trizol method (Invitrogen) according to the manufacturers instructions and was purified using RNeasy mini-columns (Qiagen, Valencia, CA). The quality of the RNA obtained from each tumour sample was assessed based on the RNA profile generated by the bioanalyzer (Agilent Inc).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Approximately 1 microgram of total RNA was processed to produce biotinylated cRNA targets.
| Sample_hyb_protocol | standard Affymetrix procedures.
| Sample_scan_protocol | standard Affymetrix procedures.
| Sample_data_processing | Raw gene expression values were obtained using Affymetrix GeneChip software and further analyzed using the DNA-Chip Analyzer (dChip).
| Sample_platform_id | GPL570
| Sample_contact_name | Qiyuan,,Li
| Sample_contact_email | qiyuan@dfci.harvard.edu
| Sample_contact_phone | 617-582-8682
| Sample_contact_department | Medical Oncology
| Sample_contact_institute | Dana Farber Cancer Institute
| Sample_contact_address | 44 Binney street
| Sample_contact_city | Boston
| Sample_contact_state | MA
| Sample_contact_zip/postal_code | 02115
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM579nnn/GSM579978/suppl/GSM579978.CEL.gz
| Sample_series_id | GSE23640
| Sample_data_row_count | 54675
| |
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