Result

Search results for the GEO ID: GSE23724

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GSM ID

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Title

Source name

Description

Characteristics

GSM585541

GPL1261

skin.wt.replicate4

dorsal skin from GR+/+ E18.5 embryo

tissue: dorsal skin genotype/variation: GR+/+ strain: B6D2/F1 age: E18.5

Gene expression data from dorsal skin of GR+/+ E18.5 embryos.

Sample_geo_accession	GSM585541
Sample_status	Public on Aug 21 2010
Sample_submission_date	Aug 20 2010
Sample_last_update_date	Aug 23 2010
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	GR-/- and GR+/+ embryo littermates were obtained by cesarean derivation at 18.5 days post-conception (the morning of the day that the vaginal plug was seen was considered as day 0.5).
Sample_growth_protocol_ch1	Mice were housed in standard temperature and humidity conditions and animal experimentation was conducted with accepted standards of humane animal care in our animal facility (Instituto de Biomedicina de Valencia IBV-CSIC).
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was obtained using the RNeasy RNA extraction kit (Qiagen, Valencia, CA). RNA integrity was tested using the Bioanalyzer 2100 (Agilent Technologies).
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Starting from an average of 5 mg total RNA, cDNA was synthesized and purified (one cycle cDNA synthesis Affy). cRNA was then biotin-labeled and purified (IVT Affy kit). After quantitation of the biotinylated cRNA, it was fragmented to perform the hybridization step as described on the webpage of the genomic platform at the Centro de Investigación del Cáncer de Salamanca (http://ubioinfo.cicancer.org).
Sample_hyb_protocol	15 mg of fragmented cRNA were used for each sample and hybridization performed as described on the webpage of the genomic platform at the Centro de Investigación del Cáncer de Salamanca (http://ubioinfo.cicancer.org).
Sample_scan_protocol	GeneChips were scanned using a GenePix 4000B scanner (Molecular Devices, Sunnyvale, CA).
Sample_data_processing	Probe set intensity values were summarized and normalized according to the RMA method using R and BioC.
Sample_platform_id	GPL1261
Sample_contact_name	Paloma,,Perez
Sample_contact_email	pperez@ibv.csic.es
Sample_contact_laboratory	Animal Models for Skin Pathology
Sample_contact_institute	Instituto Biomedicina Valencia, Consejo Superior Investigaciones Científicas
Sample_contact_address	Jaime Roig 11
Sample_contact_city	Valencia
Sample_contact_zip/postal_code	46010
Sample_contact_country	Spain
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM585nnn/GSM585541/suppl/GSM585541.CEL.gz
Sample_series_id	GSE23724
Sample_data_row_count	45101

GSM585542

GPL1261

skin.wt.replicate6

dorsal skin from GR+/+ E18.5 embryo

tissue: dorsal skin genotype/variation: GR+/+ strain: B6D2/F1 age: E18.5

Gene expression data from dorsal skin of GR+/+ E18.5 embryos.

Sample_geo_accession	GSM585542
Sample_status	Public on Aug 21 2010
Sample_submission_date	Aug 20 2010
Sample_last_update_date	Aug 23 2010
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	GR-/- and GR+/+ embryo littermates were obtained by cesarean derivation at 18.5 days post-conception (the morning of the day that the vaginal plug was seen was considered as day 0.5).
Sample_growth_protocol_ch1	Mice were housed in standard temperature and humidity conditions and animal experimentation was conducted with accepted standards of humane animal care in our animal facility (Instituto de Biomedicina de Valencia IBV-CSIC).
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was obtained using the RNeasy RNA extraction kit (Qiagen, Valencia, CA). RNA integrity was tested using the Bioanalyzer 2100 (Agilent Technologies).
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Starting from an average of 5 mg total RNA, cDNA was synthesized and purified (one cycle cDNA synthesis Affy). cRNA was then biotin-labeled and purified (IVT Affy kit). After quantitation of the biotinylated cRNA, it was fragmented to perform the hybridization step as described on the webpage of the genomic platform at the Centro de Investigación del Cáncer de Salamanca (http://ubioinfo.cicancer.org).
Sample_hyb_protocol	15 mg of fragmented cRNA were used for each sample and hybridization performed as described on the webpage of the genomic platform at the Centro de Investigación del Cáncer de Salamanca (http://ubioinfo.cicancer.org).
Sample_scan_protocol	GeneChips were scanned using a GenePix 4000B scanner (Molecular Devices, Sunnyvale, CA).
Sample_data_processing	Probe set intensity values were summarized and normalized according to the RMA method using R and BioC.
Sample_platform_id	GPL1261
Sample_contact_name	Paloma,,Perez
Sample_contact_email	pperez@ibv.csic.es
Sample_contact_laboratory	Animal Models for Skin Pathology
Sample_contact_institute	Instituto Biomedicina Valencia, Consejo Superior Investigaciones Científicas
Sample_contact_address	Jaime Roig 11
Sample_contact_city	Valencia
Sample_contact_zip/postal_code	46010
Sample_contact_country	Spain
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM585nnn/GSM585542/suppl/GSM585542.CEL.gz
Sample_series_id	GSE23724
Sample_data_row_count	45101

GSM585543

GPL1261

skin.wt.replicate28

dorsal skin from GR+/+ E18.5 embryo

tissue: dorsal skin genotype/variation: GR+/+ strain: B6D2/F1 age: E18.5

Gene expression data from dorsal skin of GR+/+ E18.5 embryos.

Sample_geo_accession	GSM585543
Sample_status	Public on Aug 21 2010
Sample_submission_date	Aug 20 2010
Sample_last_update_date	Aug 23 2010
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	GR-/- and GR+/+ embryo littermates were obtained by cesarean derivation at 18.5 days post-conception (the morning of the day that the vaginal plug was seen was considered as day 0.5).
Sample_growth_protocol_ch1	Mice were housed in standard temperature and humidity conditions and animal experimentation was conducted with accepted standards of humane animal care in our animal facility (Instituto de Biomedicina de Valencia IBV-CSIC).
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was obtained using the RNeasy RNA extraction kit (Qiagen, Valencia, CA). RNA integrity was tested using the Bioanalyzer 2100 (Agilent Technologies).
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Starting from an average of 5 mg total RNA, cDNA was synthesized and purified (one cycle cDNA synthesis Affy). cRNA was then biotin-labeled and purified (IVT Affy kit). After quantitation of the biotinylated cRNA, it was fragmented to perform the hybridization step as described on the webpage of the genomic platform at the Centro de Investigación del Cáncer de Salamanca (http://ubioinfo.cicancer.org).
Sample_hyb_protocol	15 mg of fragmented cRNA were used for each sample and hybridization performed as described on the webpage of the genomic platform at the Centro de Investigación del Cáncer de Salamanca (http://ubioinfo.cicancer.org).
Sample_scan_protocol	GeneChips were scanned using a GenePix 4000B scanner (Molecular Devices, Sunnyvale, CA).
Sample_data_processing	Probe set intensity values were summarized and normalized according to the RMA method using R and BioC.
Sample_platform_id	GPL1261
Sample_contact_name	Paloma,,Perez
Sample_contact_email	pperez@ibv.csic.es
Sample_contact_laboratory	Animal Models for Skin Pathology
Sample_contact_institute	Instituto Biomedicina Valencia, Consejo Superior Investigaciones Científicas
Sample_contact_address	Jaime Roig 11
Sample_contact_city	Valencia
Sample_contact_zip/postal_code	46010
Sample_contact_country	Spain
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM585nnn/GSM585543/suppl/GSM585543.CEL.gz
Sample_series_id	GSE23724
Sample_data_row_count	45101

GSM585544

GPL1261

skin.wt.replicate29

dorsal skin from GR+/+ E18.5 embryo

tissue: dorsal skin genotype/variation: GR+/+ strain: B6D2/F1 age: E18.5

Gene expression data from dorsal skin of GR+/+ E18.5 embryos.

Sample_geo_accession	GSM585544
Sample_status	Public on Aug 21 2010
Sample_submission_date	Aug 20 2010
Sample_last_update_date	Aug 23 2010
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	GR-/- and GR+/+ embryo littermates were obtained by cesarean derivation at 18.5 days post-conception (the morning of the day that the vaginal plug was seen was considered as day 0.5).
Sample_growth_protocol_ch1	Mice were housed in standard temperature and humidity conditions and animal experimentation was conducted with accepted standards of humane animal care in our animal facility (Instituto de Biomedicina de Valencia IBV-CSIC).
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was obtained using the RNeasy RNA extraction kit (Qiagen, Valencia, CA). RNA integrity was tested using the Bioanalyzer 2100 (Agilent Technologies).
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Starting from an average of 5 mg total RNA, cDNA was synthesized and purified (one cycle cDNA synthesis Affy). cRNA was then biotin-labeled and purified (IVT Affy kit). After quantitation of the biotinylated cRNA, it was fragmented to perform the hybridization step as described on the webpage of the genomic platform at the Centro de Investigación del Cáncer de Salamanca (http://ubioinfo.cicancer.org).
Sample_hyb_protocol	15 mg of fragmented cRNA were used for each sample and hybridization performed as described on the webpage of the genomic platform at the Centro de Investigación del Cáncer de Salamanca (http://ubioinfo.cicancer.org).
Sample_scan_protocol	GeneChips were scanned using a GenePix 4000B scanner (Molecular Devices, Sunnyvale, CA).
Sample_data_processing	Probe set intensity values were summarized and normalized according to the RMA method using R and BioC.
Sample_platform_id	GPL1261
Sample_contact_name	Paloma,,Perez
Sample_contact_email	pperez@ibv.csic.es
Sample_contact_laboratory	Animal Models for Skin Pathology
Sample_contact_institute	Instituto Biomedicina Valencia, Consejo Superior Investigaciones Científicas
Sample_contact_address	Jaime Roig 11
Sample_contact_city	Valencia
Sample_contact_zip/postal_code	46010
Sample_contact_country	Spain
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM585nnn/GSM585544/suppl/GSM585544.CEL.gz
Sample_series_id	GSE23724
Sample_data_row_count	45101

GSM585545

GPL1261

skin.KO.replicate5

dorsal skin from GR-/- E18.5 embryo

tissue: dorsal skin genotype/variation: GR-/- strain: B6D2/F1 age: E18.5

Gene expression data from dorsal skin of GR-/- E18.5 embryos.

Sample_geo_accession	GSM585545
Sample_status	Public on Aug 21 2010
Sample_submission_date	Aug 20 2010
Sample_last_update_date	Aug 23 2010
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	GR-/- and GR+/+ embryo littermates were obtained by cesarean derivation at 18.5 days post-conception (the morning of the day that the vaginal plug was seen was considered as day 0.5).
Sample_growth_protocol_ch1	Mice were housed in standard temperature and humidity conditions and animal experimentation was conducted with accepted standards of humane animal care in our animal facility (Instituto de Biomedicina de Valencia IBV-CSIC).
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was obtained using the RNeasy RNA extraction kit (Qiagen, Valencia, CA). RNA integrity was tested using the Bioanalyzer 2100 (Agilent Technologies).
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Starting from an average of 5 mg total RNA, cDNA was synthesized and purified (one cycle cDNA synthesis Affy). cRNA was then biotin-labeled and purified (IVT Affy kit). After quantitation of the biotinylated cRNA, it was fragmented to perform the hybridization step as described on the webpage of the genomic platform at the Centro de Investigación del Cáncer de Salamanca (http://ubioinfo.cicancer.org).
Sample_hyb_protocol	15 mg of fragmented cRNA were used for each sample and hybridization performed as described on the webpage of the genomic platform at the Centro de Investigación del Cáncer de Salamanca (http://ubioinfo.cicancer.org).
Sample_scan_protocol	GeneChips were scanned using a GenePix 4000B scanner (Molecular Devices, Sunnyvale, CA).
Sample_data_processing	Probe set intensity values were summarized and normalized according to the RMA method using R and BioC.
Sample_platform_id	GPL1261
Sample_contact_name	Paloma,,Perez
Sample_contact_email	pperez@ibv.csic.es
Sample_contact_laboratory	Animal Models for Skin Pathology
Sample_contact_institute	Instituto Biomedicina Valencia, Consejo Superior Investigaciones Científicas
Sample_contact_address	Jaime Roig 11
Sample_contact_city	Valencia
Sample_contact_zip/postal_code	46010
Sample_contact_country	Spain
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM585nnn/GSM585545/suppl/GSM585545.CEL.gz
Sample_series_id	GSE23724
Sample_data_row_count	45101

GSM585546

GPL1261

skin.KO.replicate17

dorsal skin from GR-/- E18.5 embryo

tissue: dorsal skin genotype/variation: GR-/- strain: B6D2/F1 age: E18.5

Gene expression data from dorsal skin of GR-/- E18.5 embryos.

Sample_geo_accession	GSM585546
Sample_status	Public on Aug 21 2010
Sample_submission_date	Aug 20 2010
Sample_last_update_date	Aug 23 2010
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	GR-/- and GR+/+ embryo littermates were obtained by cesarean derivation at 18.5 days post-conception (the morning of the day that the vaginal plug was seen was considered as day 0.5).
Sample_growth_protocol_ch1	Mice were housed in standard temperature and humidity conditions and animal experimentation was conducted with accepted standards of humane animal care in our animal facility (Instituto de Biomedicina de Valencia IBV-CSIC).
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was obtained using the RNeasy RNA extraction kit (Qiagen, Valencia, CA). RNA integrity was tested using the Bioanalyzer 2100 (Agilent Technologies).
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Starting from an average of 5 mg total RNA, cDNA was synthesized and purified (one cycle cDNA synthesis Affy). cRNA was then biotin-labeled and purified (IVT Affy kit). After quantitation of the biotinylated cRNA, it was fragmented to perform the hybridization step as described on the webpage of the genomic platform at the Centro de Investigación del Cáncer de Salamanca (http://ubioinfo.cicancer.org).
Sample_hyb_protocol	15 mg of fragmented cRNA were used for each sample and hybridization performed as described on the webpage of the genomic platform at the Centro de Investigación del Cáncer de Salamanca (http://ubioinfo.cicancer.org).
Sample_scan_protocol	GeneChips were scanned using a GenePix 4000B scanner (Molecular Devices, Sunnyvale, CA).
Sample_data_processing	Probe set intensity values were summarized and normalized according to the RMA method using R and BioC.
Sample_platform_id	GPL1261
Sample_contact_name	Paloma,,Perez
Sample_contact_email	pperez@ibv.csic.es
Sample_contact_laboratory	Animal Models for Skin Pathology
Sample_contact_institute	Instituto Biomedicina Valencia, Consejo Superior Investigaciones Científicas
Sample_contact_address	Jaime Roig 11
Sample_contact_city	Valencia
Sample_contact_zip/postal_code	46010
Sample_contact_country	Spain
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM585nnn/GSM585546/suppl/GSM585546.CEL.gz
Sample_series_id	GSE23724
Sample_data_row_count	45101

GSM585547

GPL1261

skin.KO.replicate25

dorsal skin from GR-/- E18.5 embryo

tissue: dorsal skin genotype/variation: GR-/- strain: B6D2/F1 age: E18.5

Gene expression data from dorsal skin of GR-/- E18.5 embryos.

Sample_geo_accession	GSM585547
Sample_status	Public on Aug 21 2010
Sample_submission_date	Aug 20 2010
Sample_last_update_date	Aug 23 2010
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	GR-/- and GR+/+ embryo littermates were obtained by cesarean derivation at 18.5 days post-conception (the morning of the day that the vaginal plug was seen was considered as day 0.5).
Sample_growth_protocol_ch1	Mice were housed in standard temperature and humidity conditions and animal experimentation was conducted with accepted standards of humane animal care in our animal facility (Instituto de Biomedicina de Valencia IBV-CSIC).
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was obtained using the RNeasy RNA extraction kit (Qiagen, Valencia, CA). RNA integrity was tested using the Bioanalyzer 2100 (Agilent Technologies).
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Starting from an average of 5 mg total RNA, cDNA was synthesized and purified (one cycle cDNA synthesis Affy). cRNA was then biotin-labeled and purified (IVT Affy kit). After quantitation of the biotinylated cRNA, it was fragmented to perform the hybridization step as described on the webpage of the genomic platform at the Centro de Investigación del Cáncer de Salamanca (http://ubioinfo.cicancer.org).
Sample_hyb_protocol	15 mg of fragmented cRNA were used for each sample and hybridization performed as described on the webpage of the genomic platform at the Centro de Investigación del Cáncer de Salamanca (http://ubioinfo.cicancer.org).
Sample_scan_protocol	GeneChips were scanned using a GenePix 4000B scanner (Molecular Devices, Sunnyvale, CA).
Sample_data_processing	Probe set intensity values were summarized and normalized according to the RMA method using R and BioC.
Sample_platform_id	GPL1261
Sample_contact_name	Paloma,,Perez
Sample_contact_email	pperez@ibv.csic.es
Sample_contact_laboratory	Animal Models for Skin Pathology
Sample_contact_institute	Instituto Biomedicina Valencia, Consejo Superior Investigaciones Científicas
Sample_contact_address	Jaime Roig 11
Sample_contact_city	Valencia
Sample_contact_zip/postal_code	46010
Sample_contact_country	Spain
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM585nnn/GSM585547/suppl/GSM585547.CEL.gz
Sample_series_id	GSE23724
Sample_data_row_count	45101

GSM585548

GPL1261

skin.KO.replicate26

dorsal skin from GR-/- E18.5 embryo

tissue: dorsal skin genotype/variation: GR-/- strain: B6D2/F1 age: E18.5

Gene expression data from dorsal skin of GR-/- E18.5 embryos.

Sample_geo_accession	GSM585548
Sample_status	Public on Aug 21 2010
Sample_submission_date	Aug 20 2010
Sample_last_update_date	Aug 23 2010
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	GR-/- and GR+/+ embryo littermates were obtained by cesarean derivation at 18.5 days post-conception (the morning of the day that the vaginal plug was seen was considered as day 0.5).
Sample_growth_protocol_ch1	Mice were housed in standard temperature and humidity conditions and animal experimentation was conducted with accepted standards of humane animal care in our animal facility (Instituto de Biomedicina de Valencia IBV-CSIC).
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was obtained using the RNeasy RNA extraction kit (Qiagen, Valencia, CA). RNA integrity was tested using the Bioanalyzer 2100 (Agilent Technologies).
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Starting from an average of 5 mg total RNA, cDNA was synthesized and purified (one cycle cDNA synthesis Affy). cRNA was then biotin-labeled and purified (IVT Affy kit). After quantitation of the biotinylated cRNA, it was fragmented to perform the hybridization step as described on the webpage of the genomic platform at the Centro de Investigación del Cáncer de Salamanca (http://ubioinfo.cicancer.org).
Sample_hyb_protocol	15 mg of fragmented cRNA were used for each sample and hybridization performed as described on the webpage of the genomic platform at the Centro de Investigación del Cáncer de Salamanca (http://ubioinfo.cicancer.org).
Sample_scan_protocol	GeneChips were scanned using a GenePix 4000B scanner (Molecular Devices, Sunnyvale, CA).
Sample_data_processing	Probe set intensity values were summarized and normalized according to the RMA method using R and BioC.
Sample_platform_id	GPL1261
Sample_contact_name	Paloma,,Perez
Sample_contact_email	pperez@ibv.csic.es
Sample_contact_laboratory	Animal Models for Skin Pathology
Sample_contact_institute	Instituto Biomedicina Valencia, Consejo Superior Investigaciones Científicas
Sample_contact_address	Jaime Roig 11
Sample_contact_city	Valencia
Sample_contact_zip/postal_code	46010
Sample_contact_country	Spain
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM585nnn/GSM585548/suppl/GSM585548.CEL.gz
Sample_series_id	GSE23724
Sample_data_row_count	45101

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