Search results for the GEO ID: GSE23740 |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM585868 | GPL1355 |
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liver_probiotic_rep1
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Hypercholesterolemic rat treated with probiotic Lactobacillus casei Zhang
|
tissue: liver
treatment: Lactobacillus casei Zhang
|
Gene expression data of liver of hypercholesterolemic rat treated with probiotic Lactobacillus casei Zhang
|
Sample_geo_accession | GSM585868
| Sample_status | Public on Dec 31 2011
| Sample_submission_date | Aug 21 2010
| Sample_last_update_date | Dec 31 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_growth_protocol_ch1 | Rats in high fat and probiotic treated group were fed with fat-rich diet to form hypercholesterol models in the first 30 d. In the next 30 d, in addition to fat-rich diet, rats in high fat group were daily supplied with 1 ml of normal saline and rats in probiotic treated group were daily supplied with L. casei Zhang (2 × 108cfu) dissolved in 1 ml of normal saline by intragastric administration.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 8 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Purified cRNA was broken down to 35 to 200 base fragments and incubated at 94℃ for 35 min. The fragmented and labelled cRNA was applied to the Rat Genome 230 2.0 Array. Hybridization was carried out in a mixture containing probe array controls, BSA (Invitrogen, Carlsbad, CA, USA) and herring sperm DNA (Promega, Madison, WI, USA) for 16 h at 45℃.
| Sample_scan_protocol | GeneChips were scanned using GeneArrayTM scanner 3000
| Sample_data_processing | Gene expression values were calculated from the fluorescence signal by Affymetrix GeneChip Operating Software, as described by Affymetrix GeneChip standard procedures.
| Sample_platform_id | GPL1355
| Sample_contact_name | Heping,,Zhang
| Sample_contact_institute | Inner Mongolia Agricultural University
| Sample_contact_address | No.306 Zhaowuda road
| Sample_contact_city | Hohhot
| Sample_contact_zip/postal_code | 010018
| Sample_contact_country | China
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM585nnn/GSM585868/suppl/GSM585868_Probiotic_1.CEL.gz
| Sample_series_id | GSE23740
| Sample_data_row_count | 31099
| |
|
GSM585869 | GPL1355 |
|
liver_probiotic_rep2
|
Hypercholesterolemic rat treated with probiotic Lactobacillus casei Zhang
|
tissue: liver
treatment: Lactobacillus casei Zhang
|
Gene expression data of liver of hypercholesterolemic rat treated with probiotic Lactobacillus casei Zhang
|
Sample_geo_accession | GSM585869
| Sample_status | Public on Dec 31 2011
| Sample_submission_date | Aug 21 2010
| Sample_last_update_date | Dec 31 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_growth_protocol_ch1 | Rats in high fat and probiotic treated group were fed with fat-rich diet to form hypercholesterol models in the first 30 d. In the next 30 d, in addition to fat-rich diet, rats in high fat group were daily supplied with 1 ml of normal saline and rats in probiotic treated group were daily supplied with L. casei Zhang (2 × 108cfu) dissolved in 1 ml of normal saline by intragastric administration.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 8 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Purified cRNA was broken down to 35 to 200 base fragments and incubated at 94℃ for 35 min. The fragmented and labelled cRNA was applied to the Rat Genome 230 2.0 Array. Hybridization was carried out in a mixture containing probe array controls, BSA (Invitrogen, Carlsbad, CA, USA) and herring sperm DNA (Promega, Madison, WI, USA) for 16 h at 45℃.
| Sample_scan_protocol | GeneChips were scanned using GeneArrayTM scanner 3000
| Sample_data_processing | Gene expression values were calculated from the fluorescence signal by Affymetrix GeneChip Operating Software, as described by Affymetrix GeneChip standard procedures.
| Sample_platform_id | GPL1355
| Sample_contact_name | Heping,,Zhang
| Sample_contact_institute | Inner Mongolia Agricultural University
| Sample_contact_address | No.306 Zhaowuda road
| Sample_contact_city | Hohhot
| Sample_contact_zip/postal_code | 010018
| Sample_contact_country | China
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM585nnn/GSM585869/suppl/GSM585869_Probiotic_2.CEL.gz
| Sample_series_id | GSE23740
| Sample_data_row_count | 31099
| |
|
GSM585870 | GPL1355 |
|
liver_probiotic_rep3
|
Hypercholesterolemic rat treated with probiotic Lactobacillus casei Zhang
|
tissue: liver
treatment: Lactobacillus casei Zhang
|
Gene expression data of liver of hypercholesterolemic rat treated with probiotic Lactobacillus casei Zhang
|
Sample_geo_accession | GSM585870
| Sample_status | Public on Dec 31 2011
| Sample_submission_date | Aug 21 2010
| Sample_last_update_date | Dec 31 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_growth_protocol_ch1 | Rats in high fat and probiotic treated group were fed with fat-rich diet to form hypercholesterol models in the first 30 d. In the next 30 d, in addition to fat-rich diet, rats in high fat group were daily supplied with 1 ml of normal saline and rats in probiotic treated group were daily supplied with L. casei Zhang (2 × 108cfu) dissolved in 1 ml of normal saline by intragastric administration.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 8 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Purified cRNA was broken down to 35 to 200 base fragments and incubated at 94℃ for 35 min. The fragmented and labelled cRNA was applied to the Rat Genome 230 2.0 Array. Hybridization was carried out in a mixture containing probe array controls, BSA (Invitrogen, Carlsbad, CA, USA) and herring sperm DNA (Promega, Madison, WI, USA) for 16 h at 45℃.
| Sample_scan_protocol | GeneChips were scanned using GeneArrayTM scanner 3000
| Sample_data_processing | Gene expression values were calculated from the fluorescence signal by Affymetrix GeneChip Operating Software, as described by Affymetrix GeneChip standard procedures.
| Sample_platform_id | GPL1355
| Sample_contact_name | Heping,,Zhang
| Sample_contact_institute | Inner Mongolia Agricultural University
| Sample_contact_address | No.306 Zhaowuda road
| Sample_contact_city | Hohhot
| Sample_contact_zip/postal_code | 010018
| Sample_contact_country | China
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM585nnn/GSM585870/suppl/GSM585870_Probiotic_3.CEL.gz
| Sample_series_id | GSE23740
| Sample_data_row_count | 31099
| |
|
GSM585871 | GPL1355 |
|
liver_highfat_rep1
|
Hypercholesterolemic rat as control
|
tissue: liver
treatment: high fat control
|
Gene expression data of liver of hypercholesterolemic rat
|
Sample_geo_accession | GSM585871
| Sample_status | Public on Dec 31 2011
| Sample_submission_date | Aug 21 2010
| Sample_last_update_date | Dec 31 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_growth_protocol_ch1 | Rats in high fat and probiotic treated group were fed with fat-rich diet to form hypercholesterol models in the first 30 d. In the next 30 d, in addition to fat-rich diet, rats in high fat group were daily supplied with 1 ml of normal saline and rats in probiotic treated group were daily supplied with L. casei Zhang (2 × 108cfu) dissolved in 1 ml of normal saline by intragastric administration.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 8 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Purified cRNA was broken down to 35 to 200 base fragments and incubated at 94℃ for 35 min. The fragmented and labelled cRNA was applied to the Rat Genome 230 2.0 Array. Hybridization was carried out in a mixture containing probe array controls, BSA (Invitrogen, Carlsbad, CA, USA) and herring sperm DNA (Promega, Madison, WI, USA) for 16 h at 45℃.
| Sample_scan_protocol | GeneChips were scanned using GeneArrayTM scanner 3000
| Sample_data_processing | Gene expression values were calculated from the fluorescence signal by Affymetrix GeneChip Operating Software, as described by Affymetrix GeneChip standard procedures.
| Sample_platform_id | GPL1355
| Sample_contact_name | Heping,,Zhang
| Sample_contact_institute | Inner Mongolia Agricultural University
| Sample_contact_address | No.306 Zhaowuda road
| Sample_contact_city | Hohhot
| Sample_contact_zip/postal_code | 010018
| Sample_contact_country | China
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM585nnn/GSM585871/suppl/GSM585871_Highfat_1.CEL.gz
| Sample_series_id | GSE23740
| Sample_data_row_count | 31099
| |
|
GSM585872 | GPL1355 |
|
liver_highfat_rep2
|
Hypercholesterolemic rat as control
|
tissue: liver
treatment: high fat control
|
Gene expression data of liver of hypercholesterolemic rat
|
Sample_geo_accession | GSM585872
| Sample_status | Public on Dec 31 2011
| Sample_submission_date | Aug 21 2010
| Sample_last_update_date | Dec 31 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_growth_protocol_ch1 | Rats in high fat and probiotic treated group were fed with fat-rich diet to form hypercholesterol models in the first 30 d. In the next 30 d, in addition to fat-rich diet, rats in high fat group were daily supplied with 1 ml of normal saline and rats in probiotic treated group were daily supplied with L. casei Zhang (2 × 108cfu) dissolved in 1 ml of normal saline by intragastric administration.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 8 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Purified cRNA was broken down to 35 to 200 base fragments and incubated at 94℃ for 35 min. The fragmented and labelled cRNA was applied to the Rat Genome 230 2.0 Array. Hybridization was carried out in a mixture containing probe array controls, BSA (Invitrogen, Carlsbad, CA, USA) and herring sperm DNA (Promega, Madison, WI, USA) for 16 h at 45℃.
| Sample_scan_protocol | GeneChips were scanned using GeneArrayTM scanner 3000
| Sample_data_processing | Gene expression values were calculated from the fluorescence signal by Affymetrix GeneChip Operating Software, as described by Affymetrix GeneChip standard procedures.
| Sample_platform_id | GPL1355
| Sample_contact_name | Heping,,Zhang
| Sample_contact_institute | Inner Mongolia Agricultural University
| Sample_contact_address | No.306 Zhaowuda road
| Sample_contact_city | Hohhot
| Sample_contact_zip/postal_code | 010018
| Sample_contact_country | China
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM585nnn/GSM585872/suppl/GSM585872_Highfat_2.CEL.gz
| Sample_series_id | GSE23740
| Sample_data_row_count | 31099
| |
|
GSM585873 | GPL1355 |
|
liver_highfat_rep3
|
Hypercholesterolemic rat as control
|
tissue: liver
treatment: high fat control
|
Gene expression data of liver of hypercholesterolemic rat
|
Sample_geo_accession | GSM585873
| Sample_status | Public on Dec 31 2011
| Sample_submission_date | Aug 21 2010
| Sample_last_update_date | Dec 31 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_growth_protocol_ch1 | Rats in high fat and probiotic treated group were fed with fat-rich diet to form hypercholesterol models in the first 30 d. In the next 30 d, in addition to fat-rich diet, rats in high fat group were daily supplied with 1 ml of normal saline and rats in probiotic treated group were daily supplied with L. casei Zhang (2 × 108cfu) dissolved in 1 ml of normal saline by intragastric administration.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 8 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Purified cRNA was broken down to 35 to 200 base fragments and incubated at 94℃ for 35 min. The fragmented and labelled cRNA was applied to the Rat Genome 230 2.0 Array. Hybridization was carried out in a mixture containing probe array controls, BSA (Invitrogen, Carlsbad, CA, USA) and herring sperm DNA (Promega, Madison, WI, USA) for 16 h at 45℃.
| Sample_scan_protocol | GeneChips were scanned using GeneArrayTM scanner 3000
| Sample_data_processing | Gene expression values were calculated from the fluorescence signal by Affymetrix GeneChip Operating Software, as described by Affymetrix GeneChip standard procedures.
| Sample_platform_id | GPL1355
| Sample_contact_name | Heping,,Zhang
| Sample_contact_institute | Inner Mongolia Agricultural University
| Sample_contact_address | No.306 Zhaowuda road
| Sample_contact_city | Hohhot
| Sample_contact_zip/postal_code | 010018
| Sample_contact_country | China
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM585nnn/GSM585873/suppl/GSM585873_Highfat_3.CEL.gz
| Sample_series_id | GSE23740
| Sample_data_row_count | 31099
| |
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