Search results for the GEO ID: GSE23741  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM585874 | GPL570 |
|
normal fibroblast_untreated_rep 1
|
Homo sapiens normal fibroblast_untreated
|
cell type: normal dermal fibroblast
sex: F
age: 61
passage #: 8
treatment: Untreated
|
n/a
|
| Sample_geo_accession | GSM585874
| | Sample_status | Public on Aug 22 2010
| | Sample_submission_date | Aug 21 2010
| | Sample_last_update_date | Aug 21 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Experimental samples were treated with 50 µg/ml rottlerin for 24 hours; control samples were treated with 1xPBS for 24 hours
| | Sample_growth_protocol_ch1 | Human fibroblasts were grown until confluent in 10% DMEM+10% fetal bovine serum, 1% vitamins, 2 mM glutamine, antibiotics, and fungizone in 100 mm plates until confluent, then preincubated for 24 h with 40 μg/ml ascorbic acid phosphate magnesium salt n-hydrate to optimize collagen production.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNEasy extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA was prepared using the BioArray High Yield RNA Transcript Labeling Kit according to the manufacturer's instructions
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C to Human Genome HU133A oligonucleotide array. GeneChipwere washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with GeneChip Operating Software (GCOS) version 3.0 using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.. Background correction and normalization were done using a Robust Multichip Average (RMA) with Genespring V 10.0 software.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Peter,Joseph,Wermuth
| | Sample_contact_email | peter.wermuth@jefferson.edu
| | Sample_contact_department | Dermatology and Cutaneous Biology
| | Sample_contact_institute | Thomas Jefferson University
| | Sample_contact_address | 233 South 10th Street
| | Sample_contact_city | Philadelphia
| | Sample_contact_state | PA
| | Sample_contact_zip/postal_code | 19107
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM585nnn/GSM585874/suppl/GSM585874_Untreated_Normal_control_NC-1.CEL.gz
| | Sample_series_id | GSE23741
| | Sample_data_row_count | 54675
| | |
|
GSM585875 | GPL570 |
|
normal fibroblast_untreated_rep 2
|
Homo sapiens normal fibroblast_untreated
|
cell type: normal dermal fibroblast
sex: F
age: 49
passage #: 7
treatment: Untreated
|
n/a
|
| Sample_geo_accession | GSM585875
| | Sample_status | Public on Aug 22 2010
| | Sample_submission_date | Aug 21 2010
| | Sample_last_update_date | Aug 21 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Experimental samples were treated with 50 µg/ml rottlerin for 24 hours; control samples were treated with 1xPBS for 24 hours
| | Sample_growth_protocol_ch1 | Human fibroblasts were grown until confluent in 10% DMEM+10% fetal bovine serum, 1% vitamins, 2 mM glutamine, antibiotics, and fungizone in 100 mm plates until confluent, then preincubated for 24 h with 40 μg/ml ascorbic acid phosphate magnesium salt n-hydrate to optimize collagen production.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNEasy extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA was prepared using the BioArray High Yield RNA Transcript Labeling Kit according to the manufacturer's instructions
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C to Human Genome HU133A oligonucleotide array. GeneChipwere washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with GeneChip Operating Software (GCOS) version 3.0 using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.. Background correction and normalization were done using a Robust Multichip Average (RMA) with Genespring V 10.0 software.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Peter,Joseph,Wermuth
| | Sample_contact_email | peter.wermuth@jefferson.edu
| | Sample_contact_department | Dermatology and Cutaneous Biology
| | Sample_contact_institute | Thomas Jefferson University
| | Sample_contact_address | 233 South 10th Street
| | Sample_contact_city | Philadelphia
| | Sample_contact_state | PA
| | Sample_contact_zip/postal_code | 19107
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM585nnn/GSM585875/suppl/GSM585875_Untreated_Normal_control_NC-2.CEL.gz
| | Sample_series_id | GSE23741
| | Sample_data_row_count | 54675
| | |
|
GSM585876 | GPL570 |
|
normal fibroblast_untreated_rep 3
|
Homo sapiens normal fibroblast_untreated
|
cell type: normal dermal fibroblast
sex: F
age: 55
passage #: 4
treatment: Untreated
|
n/a
|
| Sample_geo_accession | GSM585876
| | Sample_status | Public on Aug 22 2010
| | Sample_submission_date | Aug 21 2010
| | Sample_last_update_date | Aug 21 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Experimental samples were treated with 50 µg/ml rottlerin for 24 hours; control samples were treated with 1xPBS for 24 hours
| | Sample_growth_protocol_ch1 | Human fibroblasts were grown until confluent in 10% DMEM+10% fetal bovine serum, 1% vitamins, 2 mM glutamine, antibiotics, and fungizone in 100 mm plates until confluent, then preincubated for 24 h with 40 μg/ml ascorbic acid phosphate magnesium salt n-hydrate to optimize collagen production.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNEasy extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA was prepared using the BioArray High Yield RNA Transcript Labeling Kit according to the manufacturer's instructions
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C to Human Genome HU133A oligonucleotide array. GeneChipwere washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with GeneChip Operating Software (GCOS) version 3.0 using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.. Background correction and normalization were done using a Robust Multichip Average (RMA) with Genespring V 10.0 software.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Peter,Joseph,Wermuth
| | Sample_contact_email | peter.wermuth@jefferson.edu
| | Sample_contact_department | Dermatology and Cutaneous Biology
| | Sample_contact_institute | Thomas Jefferson University
| | Sample_contact_address | 233 South 10th Street
| | Sample_contact_city | Philadelphia
| | Sample_contact_state | PA
| | Sample_contact_zip/postal_code | 19107
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM585nnn/GSM585876/suppl/GSM585876_Untreated_Normal_control_NC-3.CEL.gz
| | Sample_series_id | GSE23741
| | Sample_data_row_count | 54675
| | |
|
GSM585877 | GPL570 |
|
normal fibroblast_50 µg/ml rottlerin_rep 1
|
Homo sapiens normal fibroblast_50 µg/ml rottlerin_24 hour
|
cell type: normal dermal fibroblast
sex: F
age: 61
passage #: 8
treatment: Rottlerin-treated
|
n/a
|
| Sample_geo_accession | GSM585877
| | Sample_status | Public on Aug 22 2010
| | Sample_submission_date | Aug 21 2010
| | Sample_last_update_date | Aug 21 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Experimental samples were treated with 50 µg/ml rottlerin for 24 hours; control samples were treated with 1xPBS for 24 hours
| | Sample_growth_protocol_ch1 | Human fibroblasts were grown until confluent in 10% DMEM+10% fetal bovine serum, 1% vitamins, 2 mM glutamine, antibiotics, and fungizone in 100 mm plates until confluent, then preincubated for 24 h with 40 μg/ml ascorbic acid phosphate magnesium salt n-hydrate to optimize collagen production.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNEasy extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA was prepared using the BioArray High Yield RNA Transcript Labeling Kit according to the manufacturer's instructions
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C to Human Genome HU133A oligonucleotide array. GeneChipwere washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with GeneChip Operating Software (GCOS) version 3.0 using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.. Background correction and normalization were done using a Robust Multichip Average (RMA) with Genespring V 10.0 software.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Peter,Joseph,Wermuth
| | Sample_contact_email | peter.wermuth@jefferson.edu
| | Sample_contact_department | Dermatology and Cutaneous Biology
| | Sample_contact_institute | Thomas Jefferson University
| | Sample_contact_address | 233 South 10th Street
| | Sample_contact_city | Philadelphia
| | Sample_contact_state | PA
| | Sample_contact_zip/postal_code | 19107
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM585nnn/GSM585877/suppl/GSM585877_Rottlerin-treated_Normal_,_NR-1.CEL.gz
| | Sample_series_id | GSE23741
| | Sample_data_row_count | 54675
| | |
|
GSM585878 | GPL570 |
|
normal fibroblast_50 µg/ml rottlerin_rep 2
|
Homo sapiens normal fibroblast_50 µg/ml rottlerin_24 hour
|
cell type: normal dermal fibroblast
sex: F
age: 49
passage #: 7
treatment: Rottlerin-treated
|
n/a
|
| Sample_geo_accession | GSM585878
| | Sample_status | Public on Aug 22 2010
| | Sample_submission_date | Aug 21 2010
| | Sample_last_update_date | Aug 21 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Experimental samples were treated with 50 µg/ml rottlerin for 24 hours; control samples were treated with 1xPBS for 24 hours
| | Sample_growth_protocol_ch1 | Human fibroblasts were grown until confluent in 10% DMEM+10% fetal bovine serum, 1% vitamins, 2 mM glutamine, antibiotics, and fungizone in 100 mm plates until confluent, then preincubated for 24 h with 40 μg/ml ascorbic acid phosphate magnesium salt n-hydrate to optimize collagen production.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNEasy extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA was prepared using the BioArray High Yield RNA Transcript Labeling Kit according to the manufacturer's instructions
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C to Human Genome HU133A oligonucleotide array. GeneChipwere washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with GeneChip Operating Software (GCOS) version 3.0 using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.. Background correction and normalization were done using a Robust Multichip Average (RMA) with Genespring V 10.0 software.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Peter,Joseph,Wermuth
| | Sample_contact_email | peter.wermuth@jefferson.edu
| | Sample_contact_department | Dermatology and Cutaneous Biology
| | Sample_contact_institute | Thomas Jefferson University
| | Sample_contact_address | 233 South 10th Street
| | Sample_contact_city | Philadelphia
| | Sample_contact_state | PA
| | Sample_contact_zip/postal_code | 19107
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM585nnn/GSM585878/suppl/GSM585878_Rottlerin-treated_Normal_,_NR-2.CEL.gz
| | Sample_series_id | GSE23741
| | Sample_data_row_count | 54675
| | |
|
GSM585879 | GPL570 |
|
normal fibroblast_50 µg/ml rottlerin_rep 3
|
Homo sapiens normal fibroblast_50 µg/ml rottlerin_24 hour
|
cell type: normal dermal fibroblast
sex: F
age: 55
passage #: 4
treatment: Rottlerin-treated
|
n/a
|
| Sample_geo_accession | GSM585879
| | Sample_status | Public on Aug 22 2010
| | Sample_submission_date | Aug 21 2010
| | Sample_last_update_date | Aug 21 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Experimental samples were treated with 50 µg/ml rottlerin for 24 hours; control samples were treated with 1xPBS for 24 hours
| | Sample_growth_protocol_ch1 | Human fibroblasts were grown until confluent in 10% DMEM+10% fetal bovine serum, 1% vitamins, 2 mM glutamine, antibiotics, and fungizone in 100 mm plates until confluent, then preincubated for 24 h with 40 μg/ml ascorbic acid phosphate magnesium salt n-hydrate to optimize collagen production.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNEasy extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA was prepared using the BioArray High Yield RNA Transcript Labeling Kit according to the manufacturer's instructions
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C to Human Genome HU133A oligonucleotide array. GeneChipwere washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with GeneChip Operating Software (GCOS) version 3.0 using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.. Background correction and normalization were done using a Robust Multichip Average (RMA) with Genespring V 10.0 software.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Peter,Joseph,Wermuth
| | Sample_contact_email | peter.wermuth@jefferson.edu
| | Sample_contact_department | Dermatology and Cutaneous Biology
| | Sample_contact_institute | Thomas Jefferson University
| | Sample_contact_address | 233 South 10th Street
| | Sample_contact_city | Philadelphia
| | Sample_contact_state | PA
| | Sample_contact_zip/postal_code | 19107
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM585nnn/GSM585879/suppl/GSM585879_Rottlerin-treated_Normal_,_NR-3.CEL.gz
| | Sample_series_id | GSE23741
| | Sample_data_row_count | 54675
| | |
|
GSM585880 | GPL570 |
|
systemic sclerosis fibroblast_untreated_rep 1
|
Homo sapiens systemic sclerosis fibroblast_untreated
|
cell type: systemic sclerosis fibroblast
sex: F
age: 62
passage #: 3
treatment: Untreated
|
n/a
|
| Sample_geo_accession | GSM585880
| | Sample_status | Public on Aug 22 2010
| | Sample_submission_date | Aug 21 2010
| | Sample_last_update_date | Aug 21 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Experimental samples were treated with 50 µg/ml rottlerin for 24 hours; control samples were treated with 1xPBS for 24 hours
| | Sample_growth_protocol_ch1 | Human fibroblasts were grown until confluent in 10% DMEM+10% fetal bovine serum, 1% vitamins, 2 mM glutamine, antibiotics, and fungizone in 100 mm plates until confluent, then preincubated for 24 h with 40 μg/ml ascorbic acid phosphate magnesium salt n-hydrate to optimize collagen production.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNEasy extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA was prepared using the BioArray High Yield RNA Transcript Labeling Kit according to the manufacturer's instructions
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C to Human Genome HU133A oligonucleotide array. GeneChipwere washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with GeneChip Operating Software (GCOS) version 3.0 using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.. Background correction and normalization were done using a Robust Multichip Average (RMA) with Genespring V 10.0 software.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Peter,Joseph,Wermuth
| | Sample_contact_email | peter.wermuth@jefferson.edu
| | Sample_contact_department | Dermatology and Cutaneous Biology
| | Sample_contact_institute | Thomas Jefferson University
| | Sample_contact_address | 233 South 10th Street
| | Sample_contact_city | Philadelphia
| | Sample_contact_state | PA
| | Sample_contact_zip/postal_code | 19107
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM585nnn/GSM585880/suppl/GSM585880_Untreated_SSc_control,_SC-1.CEL.gz
| | Sample_series_id | GSE23741
| | Sample_data_row_count | 54675
| | |
|
GSM585881 | GPL570 |
|
systemic sclerosis fibroblast_untreated_rep 2
|
Homo sapiens systemic sclerosis fibroblast_untreated
|
cell type: systemic sclerosis fibroblast
sex: M
age: 58
passage #: 5
treatment: Untreated
|
n/a
|
| Sample_geo_accession | GSM585881
| | Sample_status | Public on Aug 22 2010
| | Sample_submission_date | Aug 21 2010
| | Sample_last_update_date | Aug 21 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Experimental samples were treated with 50 µg/ml rottlerin for 24 hours; control samples were treated with 1xPBS for 24 hours
| | Sample_growth_protocol_ch1 | Human fibroblasts were grown until confluent in 10% DMEM+10% fetal bovine serum, 1% vitamins, 2 mM glutamine, antibiotics, and fungizone in 100 mm plates until confluent, then preincubated for 24 h with 40 μg/ml ascorbic acid phosphate magnesium salt n-hydrate to optimize collagen production.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNEasy extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA was prepared using the BioArray High Yield RNA Transcript Labeling Kit according to the manufacturer's instructions
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C to Human Genome HU133A oligonucleotide array. GeneChipwere washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with GeneChip Operating Software (GCOS) version 3.0 using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.. Background correction and normalization were done using a Robust Multichip Average (RMA) with Genespring V 10.0 software.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Peter,Joseph,Wermuth
| | Sample_contact_email | peter.wermuth@jefferson.edu
| | Sample_contact_department | Dermatology and Cutaneous Biology
| | Sample_contact_institute | Thomas Jefferson University
| | Sample_contact_address | 233 South 10th Street
| | Sample_contact_city | Philadelphia
| | Sample_contact_state | PA
| | Sample_contact_zip/postal_code | 19107
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM585nnn/GSM585881/suppl/GSM585881_Untreated_SSc_control,_SC-2.CEL.gz
| | Sample_series_id | GSE23741
| | Sample_data_row_count | 54675
| | |
|
GSM585882 | GPL570 |
|
systemic sclerosis fibroblast_untreated_rep 3
|
Homo sapiens systemic sclerosis fibroblast_untreated
|
cell type: systemic sclerosis fibroblast
sex: F
age: 55
passage #: 5
treatment: Untreated
|
n/a
|
| Sample_geo_accession | GSM585882
| | Sample_status | Public on Aug 22 2010
| | Sample_submission_date | Aug 21 2010
| | Sample_last_update_date | Aug 21 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Experimental samples were treated with 50 µg/ml rottlerin for 24 hours; control samples were treated with 1xPBS for 24 hours
| | Sample_growth_protocol_ch1 | Human fibroblasts were grown until confluent in 10% DMEM+10% fetal bovine serum, 1% vitamins, 2 mM glutamine, antibiotics, and fungizone in 100 mm plates until confluent, then preincubated for 24 h with 40 μg/ml ascorbic acid phosphate magnesium salt n-hydrate to optimize collagen production.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNEasy extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA was prepared using the BioArray High Yield RNA Transcript Labeling Kit according to the manufacturer's instructions
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C to Human Genome HU133A oligonucleotide array. GeneChipwere washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with GeneChip Operating Software (GCOS) version 3.0 using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.. Background correction and normalization were done using a Robust Multichip Average (RMA) with Genespring V 10.0 software.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Peter,Joseph,Wermuth
| | Sample_contact_email | peter.wermuth@jefferson.edu
| | Sample_contact_department | Dermatology and Cutaneous Biology
| | Sample_contact_institute | Thomas Jefferson University
| | Sample_contact_address | 233 South 10th Street
| | Sample_contact_city | Philadelphia
| | Sample_contact_state | PA
| | Sample_contact_zip/postal_code | 19107
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM585nnn/GSM585882/suppl/GSM585882_Untreated_SSc_control,_SC-3.CEL.gz
| | Sample_series_id | GSE23741
| | Sample_data_row_count | 54675
| | |
|
GSM585883 | GPL570 |
|
systemic sclerosis fibroblast_50 µg/ml rottlerin_rep 1
|
Homo sapiens systemic sclerosis fibroblast_50 µg/ml rottlerin_24 hour
|
cell type: systemic sclerosis fibroblast
sex: F
age: 62
passage #: 3
treatment: Rottlerin-treated
|
n/a
|
| Sample_geo_accession | GSM585883
| | Sample_status | Public on Aug 22 2010
| | Sample_submission_date | Aug 21 2010
| | Sample_last_update_date | Aug 21 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Experimental samples were treated with 50 µg/ml rottlerin for 24 hours; control samples were treated with 1xPBS for 24 hours
| | Sample_growth_protocol_ch1 | Human fibroblasts were grown until confluent in 10% DMEM+10% fetal bovine serum, 1% vitamins, 2 mM glutamine, antibiotics, and fungizone in 100 mm plates until confluent, then preincubated for 24 h with 40 μg/ml ascorbic acid phosphate magnesium salt n-hydrate to optimize collagen production.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNEasy extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA was prepared using the BioArray High Yield RNA Transcript Labeling Kit according to the manufacturer's instructions
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C to Human Genome HU133A oligonucleotide array. GeneChipwere washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with GeneChip Operating Software (GCOS) version 3.0 using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.. Background correction and normalization were done using a Robust Multichip Average (RMA) with Genespring V 10.0 software.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Peter,Joseph,Wermuth
| | Sample_contact_email | peter.wermuth@jefferson.edu
| | Sample_contact_department | Dermatology and Cutaneous Biology
| | Sample_contact_institute | Thomas Jefferson University
| | Sample_contact_address | 233 South 10th Street
| | Sample_contact_city | Philadelphia
| | Sample_contact_state | PA
| | Sample_contact_zip/postal_code | 19107
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM585nnn/GSM585883/suppl/GSM585883_Rottlerin-treated_SSc,_SR-1.CEL.gz
| | Sample_series_id | GSE23741
| | Sample_data_row_count | 54675
| | |
|
GSM585884 | GPL570 |
|
systemic sclerosis fibroblast_50 µg/ml rottlerin_rep 2
|
Homo sapiens systemic sclerosis fibroblast_50 µg/ml rottlerin_24 hour
|
cell type: systemic sclerosis fibroblast
sex: M
age: 58
passage #: 5
treatment: Rottlerin-treated
|
n/a
|
| Sample_geo_accession | GSM585884
| | Sample_status | Public on Aug 22 2010
| | Sample_submission_date | Aug 21 2010
| | Sample_last_update_date | Aug 21 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Experimental samples were treated with 50 µg/ml rottlerin for 24 hours; control samples were treated with 1xPBS for 24 hours
| | Sample_growth_protocol_ch1 | Human fibroblasts were grown until confluent in 10% DMEM+10% fetal bovine serum, 1% vitamins, 2 mM glutamine, antibiotics, and fungizone in 100 mm plates until confluent, then preincubated for 24 h with 40 μg/ml ascorbic acid phosphate magnesium salt n-hydrate to optimize collagen production.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNEasy extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA was prepared using the BioArray High Yield RNA Transcript Labeling Kit according to the manufacturer's instructions
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C to Human Genome HU133A oligonucleotide array. GeneChipwere washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with GeneChip Operating Software (GCOS) version 3.0 using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.. Background correction and normalization were done using a Robust Multichip Average (RMA) with Genespring V 10.0 software.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Peter,Joseph,Wermuth
| | Sample_contact_email | peter.wermuth@jefferson.edu
| | Sample_contact_department | Dermatology and Cutaneous Biology
| | Sample_contact_institute | Thomas Jefferson University
| | Sample_contact_address | 233 South 10th Street
| | Sample_contact_city | Philadelphia
| | Sample_contact_state | PA
| | Sample_contact_zip/postal_code | 19107
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM585nnn/GSM585884/suppl/GSM585884_Rottlerin-treated_SSc,_SR-2.CEL.gz
| | Sample_series_id | GSE23741
| | Sample_data_row_count | 54675
| | |
|
GSM585885 | GPL570 |
|
systemic sclerosis fibroblast_50 µg/ml rottlerin_rep 3
|
Homo sapiens systemic sclerosis fibroblast_50 µg/ml rottlerin_24 hour
|
cell type: systemic sclerosis fibroblast
sex: F
age: 55
passage #: 5
treatment: Rottlerin-treated
|
n/a
|
| Sample_geo_accession | GSM585885
| | Sample_status | Public on Aug 22 2010
| | Sample_submission_date | Aug 21 2010
| | Sample_last_update_date | Aug 21 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Experimental samples were treated with 50 µg/ml rottlerin for 24 hours; control samples were treated with 1xPBS for 24 hours
| | Sample_growth_protocol_ch1 | Human fibroblasts were grown until confluent in 10% DMEM+10% fetal bovine serum, 1% vitamins, 2 mM glutamine, antibiotics, and fungizone in 100 mm plates until confluent, then preincubated for 24 h with 40 μg/ml ascorbic acid phosphate magnesium salt n-hydrate to optimize collagen production.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNEasy extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA was prepared using the BioArray High Yield RNA Transcript Labeling Kit according to the manufacturer's instructions
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C to Human Genome HU133A oligonucleotide array. GeneChipwere washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with GeneChip Operating Software (GCOS) version 3.0 using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.. Background correction and normalization were done using a Robust Multichip Average (RMA) with Genespring V 10.0 software.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Peter,Joseph,Wermuth
| | Sample_contact_email | peter.wermuth@jefferson.edu
| | Sample_contact_department | Dermatology and Cutaneous Biology
| | Sample_contact_institute | Thomas Jefferson University
| | Sample_contact_address | 233 South 10th Street
| | Sample_contact_city | Philadelphia
| | Sample_contact_state | PA
| | Sample_contact_zip/postal_code | 19107
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM585nnn/GSM585885/suppl/GSM585885_Rottlerin-treated_SSc,_SR-3.CEL.gz
| | Sample_series_id | GSE23741
| | Sample_data_row_count | 54675
| | |
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