Result

Search results for the GEO ID: GSE23755

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Title

Source name

Description

Characteristics

GSM588310

GPL1261

unstimulated cells, biological rep1

mouse small intestinal epithelial m-ICcl2 cells, unstimulated

cell line: m-ICcl2 strain: C57BL/6 transgene: SV40 large T antigen cell type: small intestinal epithelial treatment: unstimulated

m-ICcl2 cells derived from C-section-derived fetal small intestinal tissue. unstimulated control_1 Gene expression data from unstimulated cells (control).

Sample_geo_accession	GSM588310
Sample_status	Public on Aug 29 2010
Sample_submission_date	Aug 28 2010
Sample_last_update_date	Sep 07 2010
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Cell were left untreated (control), stimulated for 6 h with 100 ng/mL LPS (E. coli D31m4, List Biochemicals Inc.), or stimulated for 6 h with LPS 100 ng/mL with subsequent incubation in LPS-free medium for another 90 h.
Sample_growth_protocol_ch1	m-ICcl2 cells were grown in supplemented medium on collagen-coated tissue flasks for days as described in Bens et al., Am J Physiol, 1996, 270(6 Pt 1):C1666-74.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	TRIzol RNA isolation reagent (Life Technologies) was used for total RNA preparation following the manufacturer's instructions. RNA quality was confirmed using an Agilent 2100 Bioanalyzer.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Probe labelling was performed according to standard protocols recommended by Affymetrix.
Sample_hyb_protocol	Hybridization was performed according to standard protocols recommended by Affymetrix.
Sample_scan_protocol	Scanning was performed using an Affymetrix GeneChip 3000 7G Scanner in combination with GCOS (Affymetrix) software according to standard protocols recommended by Affymetrix. Affymetrix GeneChip v3.2 software was used to create the CEL files.
Sample_data_processing	The data were normalized using GC-RMA using the ArrayAssist Lite (Affymetrix) software package.
Sample_platform_id	GPL1261
Sample_contact_name	Mathias,W.,Hornef
Sample_contact_email	hornef.mathias@mh-hannover.de
Sample_contact_phone	0049 511 538 9094
Sample_contact_fax	0049 511 532 4366
Sample_contact_department	Inst. of Medical Microbiology
Sample_contact_institute	Hannover Medical School
Sample_contact_address	Carl Neuberg Str. 1
Sample_contact_city	Hannover
Sample_contact_zip/postal_code	30625
Sample_contact_country	Germany
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM588nnn/GSM588310/suppl/GSM588310.CEL.gz
Sample_series_id	GSE23755
Sample_data_row_count	45101

GSM588311

GPL1261

unstimulated cells, biological rep2

mouse small intestinal epithelial m-ICcl2 cells, unstimulated

cell line: m-ICcl2 strain: C57BL/6 transgene: SV40 large T antigen cell type: small intestinal epithelial treatment: unstimulated

m-ICcl2 cells derived from C-section-derived fetal small intestinal tissue. unstimulated control_2 Gene expression data from unstimulated cells (control).

Sample_geo_accession	GSM588311
Sample_status	Public on Aug 29 2010
Sample_submission_date	Aug 28 2010
Sample_last_update_date	Sep 07 2010
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Cell were left untreated (control), stimulated for 6 h with 100 ng/mL LPS (E. coli D31m4, List Biochemicals Inc.), or stimulated for 6 h with LPS 100 ng/mL with subsequent incubation in LPS-free medium for another 90 h.
Sample_growth_protocol_ch1	m-ICcl2 cells were grown in supplemented medium on collagen-coated tissue flasks for days as described in Bens et al., Am J Physiol, 1996, 270(6 Pt 1):C1666-74.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	TRIzol RNA isolation reagent (Life Technologies) was used for total RNA preparation following the manufacturer's instructions. RNA quality was confirmed using an Agilent 2100 Bioanalyzer.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Probe labelling was performed according to standard protocols recommended by Affymetrix.
Sample_hyb_protocol	Hybridization was performed according to standard protocols recommended by Affymetrix.
Sample_scan_protocol	Scanning was performed using an Affymetrix GeneChip 3000 7G Scanner in combination with GCOS (Affymetrix) software according to standard protocols recommended by Affymetrix. Affymetrix GeneChip v3.2 software was used to create the CEL files.
Sample_data_processing	The data were normalized using GC-RMA using the ArrayAssist Lite (Affymetrix) software package.
Sample_platform_id	GPL1261
Sample_contact_name	Mathias,W.,Hornef
Sample_contact_email	hornef.mathias@mh-hannover.de
Sample_contact_phone	0049 511 538 9094
Sample_contact_fax	0049 511 532 4366
Sample_contact_department	Inst. of Medical Microbiology
Sample_contact_institute	Hannover Medical School
Sample_contact_address	Carl Neuberg Str. 1
Sample_contact_city	Hannover
Sample_contact_zip/postal_code	30625
Sample_contact_country	Germany
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM588nnn/GSM588311/suppl/GSM588311.CEL.gz
Sample_series_id	GSE23755
Sample_data_row_count	45101

GSM588312

GPL1261

unstimulated cells, biological rep3

mouse small intestinal epithelial m-ICcl2 cells, unstimulated

cell line: m-ICcl2 strain: C57BL/6 transgene: SV40 large T antigen cell type: small intestinal epithelial treatment: unstimulated

m-ICcl2 cells derived from C-section-derived fetal small intestinal tissue. unstimulated control_3 Gene expression data from unstimulated cells (control).

Sample_geo_accession	GSM588312
Sample_status	Public on Aug 29 2010
Sample_submission_date	Aug 28 2010
Sample_last_update_date	Sep 07 2010
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Cell were left untreated (control), stimulated for 6 h with 100 ng/mL LPS (E. coli D31m4, List Biochemicals Inc.), or stimulated for 6 h with LPS 100 ng/mL with subsequent incubation in LPS-free medium for another 90 h.
Sample_growth_protocol_ch1	m-ICcl2 cells were grown in supplemented medium on collagen-coated tissue flasks for days as described in Bens et al., Am J Physiol, 1996, 270(6 Pt 1):C1666-74.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	TRIzol RNA isolation reagent (Life Technologies) was used for total RNA preparation following the manufacturer's instructions. RNA quality was confirmed using an Agilent 2100 Bioanalyzer.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Probe labelling was performed according to standard protocols recommended by Affymetrix.
Sample_hyb_protocol	Hybridization was performed according to standard protocols recommended by Affymetrix.
Sample_scan_protocol	Scanning was performed using an Affymetrix GeneChip 3000 7G Scanner in combination with GCOS (Affymetrix) software according to standard protocols recommended by Affymetrix. Affymetrix GeneChip v3.2 software was used to create the CEL files.
Sample_data_processing	The data were normalized using GC-RMA using the ArrayAssist Lite (Affymetrix) software package.
Sample_platform_id	GPL1261
Sample_contact_name	Mathias,W.,Hornef
Sample_contact_email	hornef.mathias@mh-hannover.de
Sample_contact_phone	0049 511 538 9094
Sample_contact_fax	0049 511 532 4366
Sample_contact_department	Inst. of Medical Microbiology
Sample_contact_institute	Hannover Medical School
Sample_contact_address	Carl Neuberg Str. 1
Sample_contact_city	Hannover
Sample_contact_zip/postal_code	30625
Sample_contact_country	Germany
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM588nnn/GSM588312/suppl/GSM588312.CEL.gz
Sample_series_id	GSE23755
Sample_data_row_count	45101

GSM588313

GPL1261

6 h LPS-stimulated cells, biological rep1

mouse small intestinal epithelial m-ICcl2 cells, 6 h LPS

cell line: m-ICcl2 strain: C57BL/6 transgene: SV40 large T antigen cell type: small intestinal epithelial treatment: lipopolysaccharide (LPS) for 6 h

m-ICcl2 cells derived from C-section-derived fetal small intestinal tissue. LPS 6 h_1 Gene expression data from cells stimulated for 6 h with LPS 100 ng/mL (acute response).

Sample_geo_accession	GSM588313
Sample_status	Public on Aug 29 2010
Sample_submission_date	Aug 28 2010
Sample_last_update_date	Sep 07 2010
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Cell were left untreated (control), stimulated for 6 h with 100 ng/mL LPS (E. coli D31m4, List Biochemicals Inc.), or stimulated for 6 h with LPS 100 ng/mL with subsequent incubation in LPS-free medium for another 90 h.
Sample_growth_protocol_ch1	m-ICcl2 cells were grown in supplemented medium on collagen-coated tissue flasks for days as described in Bens et al., Am J Physiol, 1996, 270(6 Pt 1):C1666-74.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	TRIzol RNA isolation reagent (Life Technologies) was used for total RNA preparation following the manufacturer's instructions. RNA quality was confirmed using an Agilent 2100 Bioanalyzer.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Probe labelling was performed according to standard protocols recommended by Affymetrix.
Sample_hyb_protocol	Hybridization was performed according to standard protocols recommended by Affymetrix.
Sample_scan_protocol	Scanning was performed using an Affymetrix GeneChip 3000 7G Scanner in combination with GCOS (Affymetrix) software according to standard protocols recommended by Affymetrix. Affymetrix GeneChip v3.2 software was used to create the CEL files.
Sample_data_processing	The data were normalized using GC-RMA using the ArrayAssist Lite (Affymetrix) software package.
Sample_platform_id	GPL1261
Sample_contact_name	Mathias,W.,Hornef
Sample_contact_email	hornef.mathias@mh-hannover.de
Sample_contact_phone	0049 511 538 9094
Sample_contact_fax	0049 511 532 4366
Sample_contact_department	Inst. of Medical Microbiology
Sample_contact_institute	Hannover Medical School
Sample_contact_address	Carl Neuberg Str. 1
Sample_contact_city	Hannover
Sample_contact_zip/postal_code	30625
Sample_contact_country	Germany
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM588nnn/GSM588313/suppl/GSM588313.CEL.gz
Sample_series_id	GSE23755
Sample_data_row_count	45101

GSM588314

GPL1261

6 h LPS-stimulated cells, biological rep2

mouse small intestinal epithelial m-ICcl2 cells, 6 h LPS

cell line: m-ICcl2 strain: C57BL/6 transgene: SV40 large T antigen cell type: small intestinal epithelial treatment: lipopolysaccharide (LPS) for 6 h

m-ICcl2 cells derived from C-section-derived fetal small intestinal tissue. LPS 6 h_2 Gene expression data from cells stimulated for 6 h with LPS 100 ng/mL (acute response).

Sample_geo_accession	GSM588314
Sample_status	Public on Aug 29 2010
Sample_submission_date	Aug 28 2010
Sample_last_update_date	Sep 07 2010
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Cell were left untreated (control), stimulated for 6 h with 100 ng/mL LPS (E. coli D31m4, List Biochemicals Inc.), or stimulated for 6 h with LPS 100 ng/mL with subsequent incubation in LPS-free medium for another 90 h.
Sample_growth_protocol_ch1	m-ICcl2 cells were grown in supplemented medium on collagen-coated tissue flasks for days as described in Bens et al., Am J Physiol, 1996, 270(6 Pt 1):C1666-74.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	TRIzol RNA isolation reagent (Life Technologies) was used for total RNA preparation following the manufacturer's instructions. RNA quality was confirmed using an Agilent 2100 Bioanalyzer.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Probe labelling was performed according to standard protocols recommended by Affymetrix.
Sample_hyb_protocol	Hybridization was performed according to standard protocols recommended by Affymetrix.
Sample_scan_protocol	Scanning was performed using an Affymetrix GeneChip 3000 7G Scanner in combination with GCOS (Affymetrix) software according to standard protocols recommended by Affymetrix. Affymetrix GeneChip v3.2 software was used to create the CEL files.
Sample_data_processing	The data were normalized using GC-RMA using the ArrayAssist Lite (Affymetrix) software package.
Sample_platform_id	GPL1261
Sample_contact_name	Mathias,W.,Hornef
Sample_contact_email	hornef.mathias@mh-hannover.de
Sample_contact_phone	0049 511 538 9094
Sample_contact_fax	0049 511 532 4366
Sample_contact_department	Inst. of Medical Microbiology
Sample_contact_institute	Hannover Medical School
Sample_contact_address	Carl Neuberg Str. 1
Sample_contact_city	Hannover
Sample_contact_zip/postal_code	30625
Sample_contact_country	Germany
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM588nnn/GSM588314/suppl/GSM588314.CEL.gz
Sample_series_id	GSE23755
Sample_data_row_count	45101

GSM588315

GPL1261

6 h LPS-stimulated cells, biological rep3

mouse small intestinal epithelial m-ICcl2 cells, 6 h LPS

cell line: m-ICcl2 strain: C57BL/6 transgene: SV40 large T antigen cell type: small intestinal epithelial treatment: lipopolysaccharide (LPS) for 6 h

m-ICcl2 cells derived from C-section-derived fetal small intestinal tissue. LPS 6 h_3 Gene expression data from cells stimulated for 6 h with LPS 100 ng/mL (acute response).

Sample_geo_accession	GSM588315
Sample_status	Public on Aug 29 2010
Sample_submission_date	Aug 28 2010
Sample_last_update_date	Sep 07 2010
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Cell were left untreated (control), stimulated for 6 h with 100 ng/mL LPS (E. coli D31m4, List Biochemicals Inc.), or stimulated for 6 h with LPS 100 ng/mL with subsequent incubation in LPS-free medium for another 90 h.
Sample_growth_protocol_ch1	m-ICcl2 cells were grown in supplemented medium on collagen-coated tissue flasks for days as described in Bens et al., Am J Physiol, 1996, 270(6 Pt 1):C1666-74.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	TRIzol RNA isolation reagent (Life Technologies) was used for total RNA preparation following the manufacturer's instructions. RNA quality was confirmed using an Agilent 2100 Bioanalyzer.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Probe labelling was performed according to standard protocols recommended by Affymetrix.
Sample_hyb_protocol	Hybridization was performed according to standard protocols recommended by Affymetrix.
Sample_scan_protocol	Scanning was performed using an Affymetrix GeneChip 3000 7G Scanner in combination with GCOS (Affymetrix) software according to standard protocols recommended by Affymetrix. Affymetrix GeneChip v3.2 software was used to create the CEL files.
Sample_data_processing	The data were normalized using GC-RMA using the ArrayAssist Lite (Affymetrix) software package.
Sample_platform_id	GPL1261
Sample_contact_name	Mathias,W.,Hornef
Sample_contact_email	hornef.mathias@mh-hannover.de
Sample_contact_phone	0049 511 538 9094
Sample_contact_fax	0049 511 532 4366
Sample_contact_department	Inst. of Medical Microbiology
Sample_contact_institute	Hannover Medical School
Sample_contact_address	Carl Neuberg Str. 1
Sample_contact_city	Hannover
Sample_contact_zip/postal_code	30625
Sample_contact_country	Germany
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM588nnn/GSM588315/suppl/GSM588315.CEL.gz
Sample_series_id	GSE23755
Sample_data_row_count	45101

GSM588316

GPL1261

6 h LPS followed by 90 h in LPS-free medium, biological rep1

mouse small intestinal epithelial m-ICcl2 cells, 6 h LPS followed by 90 h LPS-free medium

cell line: m-ICcl2 strain: C57BL/6 transgene: SV40 large T antigen cell type: small intestinal epithelial treatment: lipopolysaccharide (LPS) for 6 h followed by LPS-free medium for 90 h

m-ICcl2 cells derived from C-section-derived fetal small intestinal tissue. LPS 96 h_1 Gene expression data from cells stimulated for 6 h with LPS (100 ng/mL) and subsequently incubated with LPS-free medium for 90 h (tolerant response).

Sample_geo_accession	GSM588316
Sample_status	Public on Aug 29 2010
Sample_submission_date	Aug 28 2010
Sample_last_update_date	Sep 07 2010
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Cell were left untreated (control), stimulated for 6 h with 100 ng/mL LPS (E. coli D31m4, List Biochemicals Inc.), or stimulated for 6 h with LPS 100 ng/mL with subsequent incubation in LPS-free medium for another 90 h.
Sample_growth_protocol_ch1	m-ICcl2 cells were grown in supplemented medium on collagen-coated tissue flasks for days as described in Bens et al., Am J Physiol, 1996, 270(6 Pt 1):C1666-74.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	TRIzol RNA isolation reagent (Life Technologies) was used for total RNA preparation following the manufacturer's instructions. RNA quality was confirmed using an Agilent 2100 Bioanalyzer.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Probe labelling was performed according to standard protocols recommended by Affymetrix.
Sample_hyb_protocol	Hybridization was performed according to standard protocols recommended by Affymetrix.
Sample_scan_protocol	Scanning was performed using an Affymetrix GeneChip 3000 7G Scanner in combination with GCOS (Affymetrix) software according to standard protocols recommended by Affymetrix. Affymetrix GeneChip v3.2 software was used to create the CEL files.
Sample_data_processing	The data were normalized using GC-RMA using the ArrayAssist Lite (Affymetrix) software package.
Sample_platform_id	GPL1261
Sample_contact_name	Mathias,W.,Hornef
Sample_contact_email	hornef.mathias@mh-hannover.de
Sample_contact_phone	0049 511 538 9094
Sample_contact_fax	0049 511 532 4366
Sample_contact_department	Inst. of Medical Microbiology
Sample_contact_institute	Hannover Medical School
Sample_contact_address	Carl Neuberg Str. 1
Sample_contact_city	Hannover
Sample_contact_zip/postal_code	30625
Sample_contact_country	Germany
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM588nnn/GSM588316/suppl/GSM588316.CEL.gz
Sample_series_id	GSE23755
Sample_data_row_count	45101

GSM588317

GPL1261

6 h LPS followed by 90 h in LPS-free medium, biological rep2

mouse small intestinal epithelial m-ICcl2 cells, 6 h LPS followed by 90 h LPS-free medium

cell line: m-ICcl2 strain: C57BL/6 transgene: SV40 large T antigen cell type: small intestinal epithelial treatment: lipopolysaccharide (LPS) for 6 h followed by LPS-free medium for 90 h

m-ICcl2 cells derived from C-section-derived fetal small intestinal tissue. LPS 96 h_2 Gene expression data from cells stimulated for 6 h with LPS (100 ng/mL) and subsequently incubated with LPS-free medium for 90 h (tolerant response).

Sample_geo_accession	GSM588317
Sample_status	Public on Aug 29 2010
Sample_submission_date	Aug 28 2010
Sample_last_update_date	Sep 07 2010
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Cell were left untreated (control), stimulated for 6 h with 100 ng/mL LPS (E. coli D31m4, List Biochemicals Inc.), or stimulated for 6 h with LPS 100 ng/mL with subsequent incubation in LPS-free medium for another 90 h.
Sample_growth_protocol_ch1	m-ICcl2 cells were grown in supplemented medium on collagen-coated tissue flasks for days as described in Bens et al., Am J Physiol, 1996, 270(6 Pt 1):C1666-74.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	TRIzol RNA isolation reagent (Life Technologies) was used for total RNA preparation following the manufacturer's instructions. RNA quality was confirmed using an Agilent 2100 Bioanalyzer.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Probe labelling was performed according to standard protocols recommended by Affymetrix.
Sample_hyb_protocol	Hybridization was performed according to standard protocols recommended by Affymetrix.
Sample_scan_protocol	Scanning was performed using an Affymetrix GeneChip 3000 7G Scanner in combination with GCOS (Affymetrix) software according to standard protocols recommended by Affymetrix. Affymetrix GeneChip v3.2 software was used to create the CEL files.
Sample_data_processing	The data were normalized using GC-RMA using the ArrayAssist Lite (Affymetrix) software package.
Sample_platform_id	GPL1261
Sample_contact_name	Mathias,W.,Hornef
Sample_contact_email	hornef.mathias@mh-hannover.de
Sample_contact_phone	0049 511 538 9094
Sample_contact_fax	0049 511 532 4366
Sample_contact_department	Inst. of Medical Microbiology
Sample_contact_institute	Hannover Medical School
Sample_contact_address	Carl Neuberg Str. 1
Sample_contact_city	Hannover
Sample_contact_zip/postal_code	30625
Sample_contact_country	Germany
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM588nnn/GSM588317/suppl/GSM588317.CEL.gz
Sample_series_id	GSE23755
Sample_data_row_count	45101

GSM588318

GPL1261

6 h LPS followed by 90 h in LPS-free medium, biological rep3

mouse small intestinal epithelial m-ICcl2 cells, 6 h LPS followed by 90 h LPS-free medium

cell line: m-ICcl2 strain: C57BL/6 transgene: SV40 large T antigen cell type: small intestinal epithelial treatment: lipopolysaccharide (LPS) for 6 h followed by LPS-free medium for 90 h

m-ICcl2 cells derived from C-section-derived fetal small intestinal tissue. LPS 96 h_3 Gene expression data from cells stimulated for 6 h with LPS (100 ng/mL) and subsequently incubated with LPS-free medium for 90 h (tolerant response).

Sample_geo_accession	GSM588318
Sample_status	Public on Aug 29 2010
Sample_submission_date	Aug 28 2010
Sample_last_update_date	Sep 07 2010
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Cell were left untreated (control), stimulated for 6 h with 100 ng/mL LPS (E. coli D31m4, List Biochemicals Inc.), or stimulated for 6 h with LPS 100 ng/mL with subsequent incubation in LPS-free medium for another 90 h.
Sample_growth_protocol_ch1	m-ICcl2 cells were grown in supplemented medium on collagen-coated tissue flasks for days as described in Bens et al., Am J Physiol, 1996, 270(6 Pt 1):C1666-74.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	TRIzol RNA isolation reagent (Life Technologies) was used for total RNA preparation following the manufacturer's instructions. RNA quality was confirmed using an Agilent 2100 Bioanalyzer.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Probe labelling was performed according to standard protocols recommended by Affymetrix.
Sample_hyb_protocol	Hybridization was performed according to standard protocols recommended by Affymetrix.
Sample_scan_protocol	Scanning was performed using an Affymetrix GeneChip 3000 7G Scanner in combination with GCOS (Affymetrix) software according to standard protocols recommended by Affymetrix. Affymetrix GeneChip v3.2 software was used to create the CEL files.
Sample_data_processing	The data were normalized using GC-RMA using the ArrayAssist Lite (Affymetrix) software package.
Sample_platform_id	GPL1261
Sample_contact_name	Mathias,W.,Hornef
Sample_contact_email	hornef.mathias@mh-hannover.de
Sample_contact_phone	0049 511 538 9094
Sample_contact_fax	0049 511 532 4366
Sample_contact_department	Inst. of Medical Microbiology
Sample_contact_institute	Hannover Medical School
Sample_contact_address	Carl Neuberg Str. 1
Sample_contact_city	Hannover
Sample_contact_zip/postal_code	30625
Sample_contact_country	Germany
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM588nnn/GSM588318/suppl/GSM588318.CEL.gz
Sample_series_id	GSE23755
Sample_data_row_count	45101

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