Search results for the GEO ID: GSE23764 |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM586484 | GPL570 |
|
A375M2, thick deformable collagen, rep1
|
melanoma cells extracted from collagen I
|
tumour type: melanoma
cell type: A375M2
cell morphology: rounded
treatment: none
matrix: thick deformable
|
Gene expression data from A375M2 cells in a thick deformable matrix
0506_VSM1_H_A375_def_r1.CEL
|
Sample_geo_accession | GSM586484
| Sample_status | Public on Aug 24 2010
| Sample_submission_date | Aug 23 2010
| Sample_last_update_date | Aug 23 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Rounded cells plated on deformable matrices were treated with actomyosin contractility inhibitors or protease inhibitors that increased contractility.
| Sample_growth_protocol_ch1 | Cells were plated on rigid or deformable matrices.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction was performed according to the manufacter´s instructions followed by purification with quiagen RNAeasy columns.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | cRNA were hybridized to Affymetrix GeneChip HGU133plus2 arrays using the standard Affymetrix protocol.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.Standard Affymetrix protocol.
| Sample_data_processing | Data were processed using R/Bioconductor. The RMA (Robust Multichip Average) expression measure was calculated for each probe set using the Affy package's “rma” function with default settings.
| Sample_platform_id | GPL570
| Sample_contact_name | Chris,,Marshall
| Sample_contact_email | chris.marshall@icr.ac.uk
| Sample_contact_phone | +44 207 352 9772
| Sample_contact_fax | +44 207 352 5630
| Sample_contact_laboratory | Oncogene Team
| Sample_contact_department | Cell and Molecular Biology
| Sample_contact_institute | Institute of Cancer Research
| Sample_contact_address | 237 Fulham Road
| Sample_contact_city | London
| Sample_contact_zip/postal_code | SW3 6JB
| Sample_contact_country | United Kingdom
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM586nnn/GSM586484/suppl/GSM586484.CEL.gz
| Sample_series_id | GSE23764
| Sample_data_row_count | 54675
| |
|
GSM586485 | GPL570 |
|
A375M2, thick deformable collagen, rep2
|
melanoma cells extracted from collagen I
|
tumour type: melanoma
cell type: A375M2
cell morphology: rounded
treatment: none
matrix: thick deformable
|
Gene expression data from A375M2 cells in a thick deformable matrix
0506_VSM2_H_A375_def_r2.CEL
|
Sample_geo_accession | GSM586485
| Sample_status | Public on Aug 24 2010
| Sample_submission_date | Aug 23 2010
| Sample_last_update_date | Aug 23 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Rounded cells plated on deformable matrices were treated with actomyosin contractility inhibitors or protease inhibitors that increased contractility.
| Sample_growth_protocol_ch1 | Cells were plated on rigid or deformable matrices.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction was performed according to the manufacter´s instructions followed by purification with quiagen RNAeasy columns.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | cRNA were hybridized to Affymetrix GeneChip HGU133plus2 arrays using the standard Affymetrix protocol.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.Standard Affymetrix protocol.
| Sample_data_processing | Data were processed using R/Bioconductor. The RMA (Robust Multichip Average) expression measure was calculated for each probe set using the Affy package's “rma” function with default settings.
| Sample_platform_id | GPL570
| Sample_contact_name | Chris,,Marshall
| Sample_contact_email | chris.marshall@icr.ac.uk
| Sample_contact_phone | +44 207 352 9772
| Sample_contact_fax | +44 207 352 5630
| Sample_contact_laboratory | Oncogene Team
| Sample_contact_department | Cell and Molecular Biology
| Sample_contact_institute | Institute of Cancer Research
| Sample_contact_address | 237 Fulham Road
| Sample_contact_city | London
| Sample_contact_zip/postal_code | SW3 6JB
| Sample_contact_country | United Kingdom
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM586nnn/GSM586485/suppl/GSM586485.CEL.gz
| Sample_series_id | GSE23764
| Sample_data_row_count | 54675
| |
|
GSM586486 | GPL570 |
|
A375M2, thick deformable collagen, rep3
|
melanoma cells extracted from collagen I
|
tumour type: melanoma
cell type: A375M2
cell morphology: rounded
treatment: none
matrix: thick deformable
|
Gene expression data from A375M2 cells in a thick deformable matrix
0506_VSM3_H_A375_def_r3.CEL
|
Sample_geo_accession | GSM586486
| Sample_status | Public on Aug 24 2010
| Sample_submission_date | Aug 23 2010
| Sample_last_update_date | Aug 23 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Rounded cells plated on deformable matrices were treated with actomyosin contractility inhibitors or protease inhibitors that increased contractility.
| Sample_growth_protocol_ch1 | Cells were plated on rigid or deformable matrices.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction was performed according to the manufacter´s instructions followed by purification with quiagen RNAeasy columns.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | cRNA were hybridized to Affymetrix GeneChip HGU133plus2 arrays using the standard Affymetrix protocol.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.Standard Affymetrix protocol.
| Sample_data_processing | Data were processed using R/Bioconductor. The RMA (Robust Multichip Average) expression measure was calculated for each probe set using the Affy package's “rma” function with default settings.
| Sample_platform_id | GPL570
| Sample_contact_name | Chris,,Marshall
| Sample_contact_email | chris.marshall@icr.ac.uk
| Sample_contact_phone | +44 207 352 9772
| Sample_contact_fax | +44 207 352 5630
| Sample_contact_laboratory | Oncogene Team
| Sample_contact_department | Cell and Molecular Biology
| Sample_contact_institute | Institute of Cancer Research
| Sample_contact_address | 237 Fulham Road
| Sample_contact_city | London
| Sample_contact_zip/postal_code | SW3 6JB
| Sample_contact_country | United Kingdom
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM586nnn/GSM586486/suppl/GSM586486.CEL.gz
| Sample_series_id | GSE23764
| Sample_data_row_count | 54675
| |
|
GSM586487 | GPL570 |
|
A375M2, thin rigid collagen, rep1
|
melanoma cells extracted from collagen I
|
tumour type: melanoma
cell type: A375M2
cell morphology: rounded
treatment: none
matrix: thin rigid
|
Gene expression data from A375M2 cells in a thin rigid matrix
0506_VSM4_H_A375_rig_r1.CEL
|
Sample_geo_accession | GSM586487
| Sample_status | Public on Aug 24 2010
| Sample_submission_date | Aug 23 2010
| Sample_last_update_date | Aug 23 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Rounded cells plated on deformable matrices were treated with actomyosin contractility inhibitors or protease inhibitors that increased contractility.
| Sample_growth_protocol_ch1 | Cells were plated on rigid or deformable matrices.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction was performed according to the manufacter´s instructions followed by purification with quiagen RNAeasy columns.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | cRNA were hybridized to Affymetrix GeneChip HGU133plus2 arrays using the standard Affymetrix protocol.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.Standard Affymetrix protocol.
| Sample_data_processing | Data were processed using R/Bioconductor. The RMA (Robust Multichip Average) expression measure was calculated for each probe set using the Affy package's “rma” function with default settings.
| Sample_platform_id | GPL570
| Sample_contact_name | Chris,,Marshall
| Sample_contact_email | chris.marshall@icr.ac.uk
| Sample_contact_phone | +44 207 352 9772
| Sample_contact_fax | +44 207 352 5630
| Sample_contact_laboratory | Oncogene Team
| Sample_contact_department | Cell and Molecular Biology
| Sample_contact_institute | Institute of Cancer Research
| Sample_contact_address | 237 Fulham Road
| Sample_contact_city | London
| Sample_contact_zip/postal_code | SW3 6JB
| Sample_contact_country | United Kingdom
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM586nnn/GSM586487/suppl/GSM586487.CEL.gz
| Sample_series_id | GSE23764
| Sample_data_row_count | 54675
| |
|
GSM586488 | GPL570 |
|
A375M2, thin rigid collagen, rep2
|
melanoma cells extracted from collagen I
|
tumour type: melanoma
cell type: A375M2
cell morphology: rounded
treatment: none
matrix: thin rigid
|
Gene expression data from a375m2 cells in a thin rigid matrix
0506_VSM5_H_A375_rig_r2.CEL
|
Sample_geo_accession | GSM586488
| Sample_status | Public on Aug 24 2010
| Sample_submission_date | Aug 23 2010
| Sample_last_update_date | Aug 23 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Rounded cells plated on deformable matrices were treated with actomyosin contractility inhibitors or protease inhibitors that increased contractility.
| Sample_growth_protocol_ch1 | Cells were plated on rigid or deformable matrices.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction was performed according to the manufacter´s instructions followed by purification with quiagen RNAeasy columns.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | cRNA were hybridized to Affymetrix GeneChip HGU133plus2 arrays using the standard Affymetrix protocol.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.Standard Affymetrix protocol.
| Sample_data_processing | Data were processed using R/Bioconductor. The RMA (Robust Multichip Average) expression measure was calculated for each probe set using the Affy package's “rma” function with default settings.
| Sample_platform_id | GPL570
| Sample_contact_name | Chris,,Marshall
| Sample_contact_email | chris.marshall@icr.ac.uk
| Sample_contact_phone | +44 207 352 9772
| Sample_contact_fax | +44 207 352 5630
| Sample_contact_laboratory | Oncogene Team
| Sample_contact_department | Cell and Molecular Biology
| Sample_contact_institute | Institute of Cancer Research
| Sample_contact_address | 237 Fulham Road
| Sample_contact_city | London
| Sample_contact_zip/postal_code | SW3 6JB
| Sample_contact_country | United Kingdom
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM586nnn/GSM586488/suppl/GSM586488.CEL.gz
| Sample_series_id | GSE23764
| Sample_data_row_count | 54675
| |
|
GSM586489 | GPL570 |
|
A375M2, thin rigid collagen, rep3
|
melanoma cells extracted from collagen I
|
tumour type: melanoma
cell type: A375M2
cell morphology: rounded
treatment: none
matrix: thin rigid
|
Gene expression data from a375m2 cells in a thin rigid matrix
0506_VSM6_H_A375_rig_r3.CEL
|
Sample_geo_accession | GSM586489
| Sample_status | Public on Aug 24 2010
| Sample_submission_date | Aug 23 2010
| Sample_last_update_date | Aug 23 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Rounded cells plated on deformable matrices were treated with actomyosin contractility inhibitors or protease inhibitors that increased contractility.
| Sample_growth_protocol_ch1 | Cells were plated on rigid or deformable matrices.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction was performed according to the manufacter´s instructions followed by purification with quiagen RNAeasy columns.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | cRNA were hybridized to Affymetrix GeneChip HGU133plus2 arrays using the standard Affymetrix protocol.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.Standard Affymetrix protocol.
| Sample_data_processing | Data were processed using R/Bioconductor. The RMA (Robust Multichip Average) expression measure was calculated for each probe set using the Affy package's “rma” function with default settings.
| Sample_platform_id | GPL570
| Sample_contact_name | Chris,,Marshall
| Sample_contact_email | chris.marshall@icr.ac.uk
| Sample_contact_phone | +44 207 352 9772
| Sample_contact_fax | +44 207 352 5630
| Sample_contact_laboratory | Oncogene Team
| Sample_contact_department | Cell and Molecular Biology
| Sample_contact_institute | Institute of Cancer Research
| Sample_contact_address | 237 Fulham Road
| Sample_contact_city | London
| Sample_contact_zip/postal_code | SW3 6JB
| Sample_contact_country | United Kingdom
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM586nnn/GSM586489/suppl/GSM586489.CEL.gz
| Sample_series_id | GSE23764
| Sample_data_row_count | 54675
| |
|
GSM586490 | GPL570 |
|
A375P, thick deformable collagen, rep1
|
melanoma cells extracted from collagen I
|
tumour type: melanoma
cell type: A375P
cell morphology: elongated
treatment: none
matrix: thick deformable
|
Gene expression data from A375P cells in a thick deformable matrix
0506_VSM7_H_A375_coll_bleb1_r1.CEL
|
Sample_geo_accession | GSM586490
| Sample_status | Public on Aug 24 2010
| Sample_submission_date | Aug 23 2010
| Sample_last_update_date | Aug 23 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Rounded cells plated on deformable matrices were treated with actomyosin contractility inhibitors or protease inhibitors that increased contractility.
| Sample_growth_protocol_ch1 | Cells were plated on rigid or deformable matrices.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction was performed according to the manufacter´s instructions followed by purification with quiagen RNAeasy columns.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | cRNA were hybridized to Affymetrix GeneChip HGU133plus2 arrays using the standard Affymetrix protocol.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.Standard Affymetrix protocol.
| Sample_data_processing | Data were processed using R/Bioconductor. The RMA (Robust Multichip Average) expression measure was calculated for each probe set using the Affy package's “rma” function with default settings.
| Sample_platform_id | GPL570
| Sample_contact_name | Chris,,Marshall
| Sample_contact_email | chris.marshall@icr.ac.uk
| Sample_contact_phone | +44 207 352 9772
| Sample_contact_fax | +44 207 352 5630
| Sample_contact_laboratory | Oncogene Team
| Sample_contact_department | Cell and Molecular Biology
| Sample_contact_institute | Institute of Cancer Research
| Sample_contact_address | 237 Fulham Road
| Sample_contact_city | London
| Sample_contact_zip/postal_code | SW3 6JB
| Sample_contact_country | United Kingdom
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM586nnn/GSM586490/suppl/GSM586490.CEL.gz
| Sample_series_id | GSE23764
| Sample_data_row_count | 54675
| |
|
GSM586491 | GPL570 |
|
A375P, thick deformable collagen, rep2
|
melanoma cells extracted from collagen I
|
tumour type: melanoma
cell type: A375P
cell morphology: elongated
treatment: none
matrix: thick deformable
|
Gene expression data from A375P cells in a thick deformable matrix
0506_VSM8_H_A375_coll_bleb1_r2.CEL
|
Sample_geo_accession | GSM586491
| Sample_status | Public on Aug 24 2010
| Sample_submission_date | Aug 23 2010
| Sample_last_update_date | Aug 23 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Rounded cells plated on deformable matrices were treated with actomyosin contractility inhibitors or protease inhibitors that increased contractility.
| Sample_growth_protocol_ch1 | Cells were plated on rigid or deformable matrices.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction was performed according to the manufacter´s instructions followed by purification with quiagen RNAeasy columns.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | cRNA were hybridized to Affymetrix GeneChip HGU133plus2 arrays using the standard Affymetrix protocol.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.Standard Affymetrix protocol.
| Sample_data_processing | Data were processed using R/Bioconductor. The RMA (Robust Multichip Average) expression measure was calculated for each probe set using the Affy package's “rma” function with default settings.
| Sample_platform_id | GPL570
| Sample_contact_name | Chris,,Marshall
| Sample_contact_email | chris.marshall@icr.ac.uk
| Sample_contact_phone | +44 207 352 9772
| Sample_contact_fax | +44 207 352 5630
| Sample_contact_laboratory | Oncogene Team
| Sample_contact_department | Cell and Molecular Biology
| Sample_contact_institute | Institute of Cancer Research
| Sample_contact_address | 237 Fulham Road
| Sample_contact_city | London
| Sample_contact_zip/postal_code | SW3 6JB
| Sample_contact_country | United Kingdom
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM586nnn/GSM586491/suppl/GSM586491.CEL.gz
| Sample_series_id | GSE23764
| Sample_data_row_count | 54675
| |
|
GSM586492 | GPL570 |
|
A375P, thick deformable collagen, rep3
|
melanoma cells extracted from collagen I
|
tumour type: melanoma
cell type: A375P
cell morphology: elongated
treatment: none
matrix: thick deformable
|
Gene expression data from A375P cells in a thick deformable matrix
0506_VSM9_H_A375_coll_bleb1_r3.CEL
|
Sample_geo_accession | GSM586492
| Sample_status | Public on Aug 24 2010
| Sample_submission_date | Aug 23 2010
| Sample_last_update_date | Aug 23 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Rounded cells plated on deformable matrices were treated with actomyosin contractility inhibitors or protease inhibitors that increased contractility.
| Sample_growth_protocol_ch1 | Cells were plated on rigid or deformable matrices.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction was performed according to the manufacter´s instructions followed by purification with quiagen RNAeasy columns.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | cRNA were hybridized to Affymetrix GeneChip HGU133plus2 arrays using the standard Affymetrix protocol.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.Standard Affymetrix protocol.
| Sample_data_processing | Data were processed using R/Bioconductor. The RMA (Robust Multichip Average) expression measure was calculated for each probe set using the Affy package's “rma” function with default settings.
| Sample_platform_id | GPL570
| Sample_contact_name | Chris,,Marshall
| Sample_contact_email | chris.marshall@icr.ac.uk
| Sample_contact_phone | +44 207 352 9772
| Sample_contact_fax | +44 207 352 5630
| Sample_contact_laboratory | Oncogene Team
| Sample_contact_department | Cell and Molecular Biology
| Sample_contact_institute | Institute of Cancer Research
| Sample_contact_address | 237 Fulham Road
| Sample_contact_city | London
| Sample_contact_zip/postal_code | SW3 6JB
| Sample_contact_country | United Kingdom
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM586nnn/GSM586492/suppl/GSM586492.CEL.gz
| Sample_series_id | GSE23764
| Sample_data_row_count | 54675
| |
|
GSM586493 | GPL570 |
|
A375M2, thick deformable collagen, blebbistatin, rep1
|
melanoma cells extracted from collagen I
|
tumour type: melanoma
cell type: A375M2
cell morphology: rounded
treatment: blebbistatin
matrix: thick deformable
|
Gene expression data from A375M2 cells in a thick deformable matrix and treated with Blebbistatin
0506_VSM10_H_A375_coll_bleb2_r1_2.CEL
|
Sample_geo_accession | GSM586493
| Sample_status | Public on Aug 24 2010
| Sample_submission_date | Aug 23 2010
| Sample_last_update_date | Aug 23 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Rounded cells plated on deformable matrices were treated with actomyosin contractility inhibitors or protease inhibitors that increased contractility.
| Sample_growth_protocol_ch1 | Cells were plated on rigid or deformable matrices.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction was performed according to the manufacter´s instructions followed by purification with quiagen RNAeasy columns.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | cRNA were hybridized to Affymetrix GeneChip HGU133plus2 arrays using the standard Affymetrix protocol.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.Standard Affymetrix protocol.
| Sample_data_processing | Data were processed using R/Bioconductor. The RMA (Robust Multichip Average) expression measure was calculated for each probe set using the Affy package's “rma” function with default settings.
| Sample_platform_id | GPL570
| Sample_contact_name | Chris,,Marshall
| Sample_contact_email | chris.marshall@icr.ac.uk
| Sample_contact_phone | +44 207 352 9772
| Sample_contact_fax | +44 207 352 5630
| Sample_contact_laboratory | Oncogene Team
| Sample_contact_department | Cell and Molecular Biology
| Sample_contact_institute | Institute of Cancer Research
| Sample_contact_address | 237 Fulham Road
| Sample_contact_city | London
| Sample_contact_zip/postal_code | SW3 6JB
| Sample_contact_country | United Kingdom
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM586nnn/GSM586493/suppl/GSM586493.CEL.gz
| Sample_series_id | GSE23764
| Sample_data_row_count | 54675
| |
|
GSM586494 | GPL570 |
|
A375M2, thick deformable collagen, blebbistatin, rep2
|
melanoma cells extracted from collagen I
|
tumour type: melanoma
cell type: A375M2
cell morphology: rounded
treatment: blebbistatin
matrix: thick deformable
|
Gene expression data from A375M2 cells in a thick deformable matrix and treated with Blebbistatin
0506_VSM11_H_A375_coll_bleb2_r2.CEL
|
Sample_geo_accession | GSM586494
| Sample_status | Public on Aug 24 2010
| Sample_submission_date | Aug 23 2010
| Sample_last_update_date | Aug 23 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Rounded cells plated on deformable matrices were treated with actomyosin contractility inhibitors or protease inhibitors that increased contractility.
| Sample_growth_protocol_ch1 | Cells were plated on rigid or deformable matrices.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction was performed according to the manufacter´s instructions followed by purification with quiagen RNAeasy columns.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | cRNA were hybridized to Affymetrix GeneChip HGU133plus2 arrays using the standard Affymetrix protocol.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.Standard Affymetrix protocol.
| Sample_data_processing | Data were processed using R/Bioconductor. The RMA (Robust Multichip Average) expression measure was calculated for each probe set using the Affy package's “rma” function with default settings.
| Sample_platform_id | GPL570
| Sample_contact_name | Chris,,Marshall
| Sample_contact_email | chris.marshall@icr.ac.uk
| Sample_contact_phone | +44 207 352 9772
| Sample_contact_fax | +44 207 352 5630
| Sample_contact_laboratory | Oncogene Team
| Sample_contact_department | Cell and Molecular Biology
| Sample_contact_institute | Institute of Cancer Research
| Sample_contact_address | 237 Fulham Road
| Sample_contact_city | London
| Sample_contact_zip/postal_code | SW3 6JB
| Sample_contact_country | United Kingdom
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM586nnn/GSM586494/suppl/GSM586494.CEL.gz
| Sample_series_id | GSE23764
| Sample_data_row_count | 54675
| |
|
GSM586495 | GPL570 |
|
A375M2, thick deformable collagen, blebbistatin, rep3
|
melanoma cells extracted from collagen I
|
tumour type: melanoma
cell type: A375M2
cell morphology: rounded
treatment: blebbistatin
matrix: thick deformable
|
Gene expression data from A375M2 cells in a thick deformable matrix and treated with Blebbistatin
0506_VSM12_H_A375_coll_bleb2_r3.CEL
|
Sample_geo_accession | GSM586495
| Sample_status | Public on Aug 24 2010
| Sample_submission_date | Aug 23 2010
| Sample_last_update_date | Aug 23 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Rounded cells plated on deformable matrices were treated with actomyosin contractility inhibitors or protease inhibitors that increased contractility.
| Sample_growth_protocol_ch1 | Cells were plated on rigid or deformable matrices.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction was performed according to the manufacter´s instructions followed by purification with quiagen RNAeasy columns.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | cRNA were hybridized to Affymetrix GeneChip HGU133plus2 arrays using the standard Affymetrix protocol.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.Standard Affymetrix protocol.
| Sample_data_processing | Data were processed using R/Bioconductor. The RMA (Robust Multichip Average) expression measure was calculated for each probe set using the Affy package's “rma” function with default settings.
| Sample_platform_id | GPL570
| Sample_contact_name | Chris,,Marshall
| Sample_contact_email | chris.marshall@icr.ac.uk
| Sample_contact_phone | +44 207 352 9772
| Sample_contact_fax | +44 207 352 5630
| Sample_contact_laboratory | Oncogene Team
| Sample_contact_department | Cell and Molecular Biology
| Sample_contact_institute | Institute of Cancer Research
| Sample_contact_address | 237 Fulham Road
| Sample_contact_city | London
| Sample_contact_zip/postal_code | SW3 6JB
| Sample_contact_country | United Kingdom
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM586nnn/GSM586495/suppl/GSM586495.CEL.gz
| Sample_series_id | GSE23764
| Sample_data_row_count | 54675
| |
|
GSM586496 | GPL570 |
|
A375M2, thick deformable collagen, Y27632, rep1
|
melanoma cells extracted from collagen I
|
tumour type: melanoma
cell type: A375M2
cell morphology: rounded
treatment: Y27632
matrix: thick deformable
|
Gene expression data from A375M2 cells in a thick deformable matrix and treated with Y27632
0506_VSM13_H_A375_con_Y27632_r1.CEL
|
Sample_geo_accession | GSM586496
| Sample_status | Public on Aug 24 2010
| Sample_submission_date | Aug 23 2010
| Sample_last_update_date | Aug 23 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Rounded cells plated on deformable matrices were treated with actomyosin contractility inhibitors or protease inhibitors that increased contractility.
| Sample_growth_protocol_ch1 | Cells were plated on rigid or deformable matrices.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction was performed according to the manufacter´s instructions followed by purification with quiagen RNAeasy columns.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | cRNA were hybridized to Affymetrix GeneChip HGU133plus2 arrays using the standard Affymetrix protocol.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.Standard Affymetrix protocol.
| Sample_data_processing | Data were processed using R/Bioconductor. The RMA (Robust Multichip Average) expression measure was calculated for each probe set using the Affy package's “rma” function with default settings.
| Sample_platform_id | GPL570
| Sample_contact_name | Chris,,Marshall
| Sample_contact_email | chris.marshall@icr.ac.uk
| Sample_contact_phone | +44 207 352 9772
| Sample_contact_fax | +44 207 352 5630
| Sample_contact_laboratory | Oncogene Team
| Sample_contact_department | Cell and Molecular Biology
| Sample_contact_institute | Institute of Cancer Research
| Sample_contact_address | 237 Fulham Road
| Sample_contact_city | London
| Sample_contact_zip/postal_code | SW3 6JB
| Sample_contact_country | United Kingdom
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM586nnn/GSM586496/suppl/GSM586496.CEL.gz
| Sample_series_id | GSE23764
| Sample_data_row_count | 54675
| |
|
GSM586497 | GPL570 |
|
A375M2, thick deformable collagen, Y27632, rep2
|
melanoma cells extracted from collagen I
|
tumour type: melanoma
cell type: A375M2
cell morphology: rounded
treatment: Y27632
matrix: thick deformable
|
Gene expression data from A375M2 cells in a thick deformable matrix and treated with Y27632
0506_VSM14_H_A375_con_Y27632_r2.CEL
|
Sample_geo_accession | GSM586497
| Sample_status | Public on Aug 24 2010
| Sample_submission_date | Aug 23 2010
| Sample_last_update_date | Aug 23 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Rounded cells plated on deformable matrices were treated with actomyosin contractility inhibitors or protease inhibitors that increased contractility.
| Sample_growth_protocol_ch1 | Cells were plated on rigid or deformable matrices.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction was performed according to the manufacter´s instructions followed by purification with quiagen RNAeasy columns.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | cRNA were hybridized to Affymetrix GeneChip HGU133plus2 arrays using the standard Affymetrix protocol.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.Standard Affymetrix protocol.
| Sample_data_processing | Data were processed using R/Bioconductor. The RMA (Robust Multichip Average) expression measure was calculated for each probe set using the Affy package's “rma” function with default settings.
| Sample_platform_id | GPL570
| Sample_contact_name | Chris,,Marshall
| Sample_contact_email | chris.marshall@icr.ac.uk
| Sample_contact_phone | +44 207 352 9772
| Sample_contact_fax | +44 207 352 5630
| Sample_contact_laboratory | Oncogene Team
| Sample_contact_department | Cell and Molecular Biology
| Sample_contact_institute | Institute of Cancer Research
| Sample_contact_address | 237 Fulham Road
| Sample_contact_city | London
| Sample_contact_zip/postal_code | SW3 6JB
| Sample_contact_country | United Kingdom
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM586nnn/GSM586497/suppl/GSM586497.CEL.gz
| Sample_series_id | GSE23764
| Sample_data_row_count | 54675
| |
|
GSM586498 | GPL570 |
|
A375M2, thick deformable collagen, Y27632, rep3
|
melanoma cells extracted from collagen I
|
tumour type: melanoma
cell type: A375M2
cell morphology: rounded
treatment: Y27632
matrix: thick deformable
|
Gene expression data from A375M2 cells in a thick deformable matrix and treated with Y27632
0506_VSM15_H_A375_con_Y27632_r3.CEL
|
Sample_geo_accession | GSM586498
| Sample_status | Public on Aug 24 2010
| Sample_submission_date | Aug 23 2010
| Sample_last_update_date | Aug 23 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Rounded cells plated on deformable matrices were treated with actomyosin contractility inhibitors or protease inhibitors that increased contractility.
| Sample_growth_protocol_ch1 | Cells were plated on rigid or deformable matrices.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction was performed according to the manufacter´s instructions followed by purification with quiagen RNAeasy columns.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | cRNA were hybridized to Affymetrix GeneChip HGU133plus2 arrays using the standard Affymetrix protocol.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.Standard Affymetrix protocol.
| Sample_data_processing | Data were processed using R/Bioconductor. The RMA (Robust Multichip Average) expression measure was calculated for each probe set using the Affy package's “rma” function with default settings.
| Sample_platform_id | GPL570
| Sample_contact_name | Chris,,Marshall
| Sample_contact_email | chris.marshall@icr.ac.uk
| Sample_contact_phone | +44 207 352 9772
| Sample_contact_fax | +44 207 352 5630
| Sample_contact_laboratory | Oncogene Team
| Sample_contact_department | Cell and Molecular Biology
| Sample_contact_institute | Institute of Cancer Research
| Sample_contact_address | 237 Fulham Road
| Sample_contact_city | London
| Sample_contact_zip/postal_code | SW3 6JB
| Sample_contact_country | United Kingdom
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM586nnn/GSM586498/suppl/GSM586498.CEL.gz
| Sample_series_id | GSE23764
| Sample_data_row_count | 54675
| |
|
GSM586499 | GPL570 |
|
A375M2, thick deformable collagen, H1152, rep1
|
melanoma cells extracted from collagen I
|
tumour type: melanoma
cell type: A375M2
cell morphology: rounded
treatment: H1152
matrix: thick deformable
|
Gene expression data from A375M2 cells in a thick deformable matrix and treated with H1152
0606_VSM25_H_A375_con_H1152_r1_3.CEL
|
Sample_geo_accession | GSM586499
| Sample_status | Public on Aug 24 2010
| Sample_submission_date | Aug 23 2010
| Sample_last_update_date | Aug 23 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Rounded cells plated on deformable matrices were treated with actomyosin contractility inhibitors or protease inhibitors that increased contractility.
| Sample_growth_protocol_ch1 | Cells were plated on rigid or deformable matrices.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction was performed according to the manufacter´s instructions followed by purification with quiagen RNAeasy columns.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | cRNA were hybridized to Affymetrix GeneChip HGU133plus2 arrays using the standard Affymetrix protocol.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.Standard Affymetrix protocol.
| Sample_data_processing | Data were processed using R/Bioconductor. The RMA (Robust Multichip Average) expression measure was calculated for each probe set using the Affy package's “rma” function with default settings.
| Sample_platform_id | GPL570
| Sample_contact_name | Chris,,Marshall
| Sample_contact_email | chris.marshall@icr.ac.uk
| Sample_contact_phone | +44 207 352 9772
| Sample_contact_fax | +44 207 352 5630
| Sample_contact_laboratory | Oncogene Team
| Sample_contact_department | Cell and Molecular Biology
| Sample_contact_institute | Institute of Cancer Research
| Sample_contact_address | 237 Fulham Road
| Sample_contact_city | London
| Sample_contact_zip/postal_code | SW3 6JB
| Sample_contact_country | United Kingdom
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM586nnn/GSM586499/suppl/GSM586499.CEL.gz
| Sample_series_id | GSE23764
| Sample_data_row_count | 54675
| |
|
GSM586500 | GPL570 |
|
A375M2, thick deformable collagen, H1152, rep2
|
melanoma cells extracted from collagen I
|
tumour type: melanoma
cell type: A375M2
cell morphology: rounded
treatment: H1152
matrix: thick deformable
|
Gene expression data from A375M2 cells in a thick deformable matrix and treated with H1152
0606_VSM26_H_A375_con_H1152_r2_3.CEL
|
Sample_geo_accession | GSM586500
| Sample_status | Public on Aug 24 2010
| Sample_submission_date | Aug 23 2010
| Sample_last_update_date | Aug 23 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Rounded cells plated on deformable matrices were treated with actomyosin contractility inhibitors or protease inhibitors that increased contractility.
| Sample_growth_protocol_ch1 | Cells were plated on rigid or deformable matrices.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction was performed according to the manufacter´s instructions followed by purification with quiagen RNAeasy columns.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | cRNA were hybridized to Affymetrix GeneChip HGU133plus2 arrays using the standard Affymetrix protocol.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.Standard Affymetrix protocol.
| Sample_data_processing | Data were processed using R/Bioconductor. The RMA (Robust Multichip Average) expression measure was calculated for each probe set using the Affy package's “rma” function with default settings.
| Sample_platform_id | GPL570
| Sample_contact_name | Chris,,Marshall
| Sample_contact_email | chris.marshall@icr.ac.uk
| Sample_contact_phone | +44 207 352 9772
| Sample_contact_fax | +44 207 352 5630
| Sample_contact_laboratory | Oncogene Team
| Sample_contact_department | Cell and Molecular Biology
| Sample_contact_institute | Institute of Cancer Research
| Sample_contact_address | 237 Fulham Road
| Sample_contact_city | London
| Sample_contact_zip/postal_code | SW3 6JB
| Sample_contact_country | United Kingdom
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM586nnn/GSM586500/suppl/GSM586500.CEL.gz
| Sample_series_id | GSE23764
| Sample_data_row_count | 54675
| |
|
GSM586501 | GPL570 |
|
A375M2, thick deformable collagen, H1152, rep3
|
melanoma cells extracted from collagen I
|
tumour type: melanoma
cell type: A375M2
cell morphology: rounded
treatment: H1152
matrix: thick deformable
|
Gene expression data from A375M2 cells in a thick deformable matrix and treated with H1152
0606_VSM27_H_A375_con_H1152_r3_2.CEL
|
Sample_geo_accession | GSM586501
| Sample_status | Public on Aug 24 2010
| Sample_submission_date | Aug 23 2010
| Sample_last_update_date | Aug 23 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Rounded cells plated on deformable matrices were treated with actomyosin contractility inhibitors or protease inhibitors that increased contractility.
| Sample_growth_protocol_ch1 | Cells were plated on rigid or deformable matrices.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction was performed according to the manufacter´s instructions followed by purification with quiagen RNAeasy columns.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | cRNA were hybridized to Affymetrix GeneChip HGU133plus2 arrays using the standard Affymetrix protocol.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.Standard Affymetrix protocol.
| Sample_data_processing | Data were processed using R/Bioconductor. The RMA (Robust Multichip Average) expression measure was calculated for each probe set using the Affy package's “rma” function with default settings.
| Sample_platform_id | GPL570
| Sample_contact_name | Chris,,Marshall
| Sample_contact_email | chris.marshall@icr.ac.uk
| Sample_contact_phone | +44 207 352 9772
| Sample_contact_fax | +44 207 352 5630
| Sample_contact_laboratory | Oncogene Team
| Sample_contact_department | Cell and Molecular Biology
| Sample_contact_institute | Institute of Cancer Research
| Sample_contact_address | 237 Fulham Road
| Sample_contact_city | London
| Sample_contact_zip/postal_code | SW3 6JB
| Sample_contact_country | United Kingdom
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM586nnn/GSM586501/suppl/GSM586501.CEL.gz
| Sample_series_id | GSE23764
| Sample_data_row_count | 54675
| |
|
|
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