Search results for the GEO ID: GSE23923 |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM589801 | GPL1261 |
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Luc control esiRNA, biological rep1
|
R1/E ESC
|
cell type: R1/E ESC
RNAi: Luciferase
|
Gene expression data from mouse ESC after 48h RNAi against Luciferase
|
Sample_geo_accession | GSM589801
| Sample_status | Public on May 19 2011
| Sample_submission_date | Sep 01 2010
| Sample_last_update_date | May 19 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | R1/E ES cells were reversely transfected in a 12well plate using 800ng esiRNA and 2ul Lipofectamine2000 (Invitrogen) and 80000cells/well. Procedure according to manufacture's protocol
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNeasy Mini Kit protocol including DNaseI digest
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 250ng total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 12.5 ug of cRNA were hybridized for 16 hr at 45C on Mouse 430 2 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| Sample_data_processing | The data were normalized with GC-RMA method using Partek Genomics Suite 6.4 (6.09.0129). Properties: quantile normalizytion, GCRMA background correction, median polish probe set summarization
| Sample_platform_id | GPL1261
| Sample_contact_name | Maciej,,Paszkowski-Rogacz
| Sample_contact_laboratory | Buchholz
| Sample_contact_institute | MPI-CBG
| Sample_contact_address | Pfotenhauerstr. 108
| Sample_contact_city | Dresden
| Sample_contact_zip/postal_code | 01307
| Sample_contact_country | Germany
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM589nnn/GSM589801/suppl/GSM589801.CEL.gz
| Sample_series_id | GSE23923
| Sample_series_id | GSE24030
| Sample_data_row_count | 45101
| |
|
GSM589802 | GPL1261 |
|
Luc control esiRNA, biological rep2
|
R1/E ESC
|
cell type: R1/E ESC
RNAi: Luciferase
|
Gene expression data from mouse ESC after 48h RNAi against Luciferase
|
Sample_geo_accession | GSM589802
| Sample_status | Public on May 19 2011
| Sample_submission_date | Sep 01 2010
| Sample_last_update_date | May 19 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | R1/E ES cells were reversely transfected in a 12well plate using 800ng esiRNA and 2ul Lipofectamine2000 (Invitrogen) and 80000cells/well. Procedure according to manufacture's protocol
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNeasy Mini Kit protocol including DNaseI digest
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 250ng total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 12.5 ug of cRNA were hybridized for 16 hr at 45C on Mouse 430 2 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| Sample_data_processing | The data were normalized with GC-RMA method using Partek Genomics Suite 6.4 (6.09.0129). Properties: quantile normalizytion, GCRMA background correction, median polish probe set summarization
| Sample_platform_id | GPL1261
| Sample_contact_name | Maciej,,Paszkowski-Rogacz
| Sample_contact_laboratory | Buchholz
| Sample_contact_institute | MPI-CBG
| Sample_contact_address | Pfotenhauerstr. 108
| Sample_contact_city | Dresden
| Sample_contact_zip/postal_code | 01307
| Sample_contact_country | Germany
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM589nnn/GSM589802/suppl/GSM589802.CEL.gz
| Sample_series_id | GSE23923
| Sample_series_id | GSE24030
| Sample_data_row_count | 45101
| |
|
GSM589803 | GPL1261 |
|
Luc control esiRNA, biological rep3
|
R1/E ESC
|
cell type: R1/E ESC
RNAi: Luciferase
|
Gene expression data from mouse ESC after 48h RNAi against Luciferase
|
Sample_geo_accession | GSM589803
| Sample_status | Public on May 19 2011
| Sample_submission_date | Sep 01 2010
| Sample_last_update_date | May 19 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | R1/E ES cells were reversely transfected in a 12well plate using 800ng esiRNA and 2ul Lipofectamine2000 (Invitrogen) and 80000cells/well. Procedure according to manufacture's protocol
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNeasy Mini Kit protocol including DNaseI digest
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 250ng total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 12.5 ug of cRNA were hybridized for 16 hr at 45C on Mouse 430 2 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| Sample_data_processing | The data were normalized with GC-RMA method using Partek Genomics Suite 6.4 (6.09.0129). Properties: quantile normalizytion, GCRMA background correction, median polish probe set summarization
| Sample_platform_id | GPL1261
| Sample_contact_name | Maciej,,Paszkowski-Rogacz
| Sample_contact_laboratory | Buchholz
| Sample_contact_institute | MPI-CBG
| Sample_contact_address | Pfotenhauerstr. 108
| Sample_contact_city | Dresden
| Sample_contact_zip/postal_code | 01307
| Sample_contact_country | Germany
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM589nnn/GSM589803/suppl/GSM589803.CEL.gz
| Sample_series_id | GSE23923
| Sample_series_id | GSE24030
| Sample_data_row_count | 45101
| |
|
GSM589804 | GPL1261 |
|
Luc control esiRNA, biological rep4
|
R1/E ESC
|
cell type: R1/E ESC
RNAi: Luciferase
|
Gene expression data from mouse ESC after 48h RNAi against Luciferase
|
Sample_geo_accession | GSM589804
| Sample_status | Public on May 19 2011
| Sample_submission_date | Sep 01 2010
| Sample_last_update_date | May 19 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | R1/E ES cells were reversely transfected in a 12well plate using 800ng esiRNA and 2ul Lipofectamine2000 (Invitrogen) and 80000cells/well. Procedure according to manufacture's protocol
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNeasy Mini Kit protocol including DNaseI digest
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 250ng total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 12.5 ug of cRNA were hybridized for 16 hr at 45C on Mouse 430 2 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| Sample_data_processing | The data were normalized with GC-RMA method using Partek Genomics Suite 6.4 (6.09.0129). Properties: quantile normalizytion, GCRMA background correction, median polish probe set summarization
| Sample_platform_id | GPL1261
| Sample_contact_name | Maciej,,Paszkowski-Rogacz
| Sample_contact_laboratory | Buchholz
| Sample_contact_institute | MPI-CBG
| Sample_contact_address | Pfotenhauerstr. 108
| Sample_contact_city | Dresden
| Sample_contact_zip/postal_code | 01307
| Sample_contact_country | Germany
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM589nnn/GSM589804/suppl/GSM589804.CEL.gz
| Sample_series_id | GSE23923
| Sample_series_id | GSE24030
| Sample_data_row_count | 45101
| |
|
GSM589805 | GPL1261 |
|
Rad21 esiRNA, biological rep1
|
R1/E ESC
|
cell type: R1/E ESC
RNAi: Rad21
|
Gene expression data from mouse ESC after 48h RNAi against Rad21
|
Sample_geo_accession | GSM589805
| Sample_status | Public on May 19 2011
| Sample_submission_date | Sep 01 2010
| Sample_last_update_date | May 19 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | R1/E ES cells were reversely transfected in a 12well plate using 800ng esiRNA and 2ul Lipofectamine2000 (Invitrogen) and 80000cells/well. Procedure according to manufacture's protocol
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNeasy Mini Kit protocol including DNaseI digest
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 250ng total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 12.5 ug of cRNA were hybridized for 16 hr at 45C on Mouse 430 2 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| Sample_data_processing | The data were normalized with GC-RMA method using Partek Genomics Suite 6.4 (6.09.0129). Properties: quantile normalizytion, GCRMA background correction, median polish probe set summarization
| Sample_platform_id | GPL1261
| Sample_contact_name | Maciej,,Paszkowski-Rogacz
| Sample_contact_laboratory | Buchholz
| Sample_contact_institute | MPI-CBG
| Sample_contact_address | Pfotenhauerstr. 108
| Sample_contact_city | Dresden
| Sample_contact_zip/postal_code | 01307
| Sample_contact_country | Germany
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM589nnn/GSM589805/suppl/GSM589805.CEL.gz
| Sample_series_id | GSE23923
| Sample_series_id | GSE24030
| Sample_data_row_count | 45101
| |
|
GSM589806 | GPL1261 |
|
Rad21 esiRNA, biological rep2
|
R1/E ESC
|
cell type: R1/E ESC
RNAi: Rad21
|
Gene expression data from mouse ESC after 48h RNAi against Rad21
|
Sample_geo_accession | GSM589806
| Sample_status | Public on May 19 2011
| Sample_submission_date | Sep 01 2010
| Sample_last_update_date | May 19 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | R1/E ES cells were reversely transfected in a 12well plate using 800ng esiRNA and 2ul Lipofectamine2000 (Invitrogen) and 80000cells/well. Procedure according to manufacture's protocol
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNeasy Mini Kit protocol including DNaseI digest
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 250ng total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 12.5 ug of cRNA were hybridized for 16 hr at 45C on Mouse 430 2 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| Sample_data_processing | The data were normalized with GC-RMA method using Partek Genomics Suite 6.4 (6.09.0129). Properties: quantile normalizytion, GCRMA background correction, median polish probe set summarization
| Sample_platform_id | GPL1261
| Sample_contact_name | Maciej,,Paszkowski-Rogacz
| Sample_contact_laboratory | Buchholz
| Sample_contact_institute | MPI-CBG
| Sample_contact_address | Pfotenhauerstr. 108
| Sample_contact_city | Dresden
| Sample_contact_zip/postal_code | 01307
| Sample_contact_country | Germany
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM589nnn/GSM589806/suppl/GSM589806.CEL.gz
| Sample_series_id | GSE23923
| Sample_series_id | GSE24030
| Sample_data_row_count | 45101
| |
|
GSM589807 | GPL1261 |
|
Rad21 esiRNA, biological rep3
|
R1/E ESC
|
cell type: R1/E ESC
RNAi: Rad21
|
Gene expression data from mouse ESC after 48h RNAi against Rad21
|
Sample_geo_accession | GSM589807
| Sample_status | Public on May 19 2011
| Sample_submission_date | Sep 01 2010
| Sample_last_update_date | May 19 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | R1/E ES cells were reversely transfected in a 12well plate using 800ng esiRNA and 2ul Lipofectamine2000 (Invitrogen) and 80000cells/well. Procedure according to manufacture's protocol
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | RNeasy Mini Kit protocol including DNaseI digest
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 250ng total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 12.5 ug of cRNA were hybridized for 16 hr at 45C on Mouse 430 2 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| Sample_data_processing | The data were normalized with GC-RMA method using Partek Genomics Suite 6.4 (6.09.0129). Properties: quantile normalizytion, GCRMA background correction, median polish probe set summarization
| Sample_platform_id | GPL1261
| Sample_contact_name | Maciej,,Paszkowski-Rogacz
| Sample_contact_laboratory | Buchholz
| Sample_contact_institute | MPI-CBG
| Sample_contact_address | Pfotenhauerstr. 108
| Sample_contact_city | Dresden
| Sample_contact_zip/postal_code | 01307
| Sample_contact_country | Germany
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM589nnn/GSM589807/suppl/GSM589807.CEL.gz
| Sample_series_id | GSE23923
| Sample_series_id | GSE24030
| Sample_data_row_count | 45101
| |
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