Search results for the GEO ID: GSE23925  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM589872 | GPL1261 |
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light zone, biological replicate 1
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day 7 germinal center light zone cells
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tissue: lymph node
cell type: germinal center B cells
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Gene expression data from sorted B cells photoactivated in the GC light zone
|
| Sample_geo_accession | GSM589872
| | Sample_status | Public on Sep 07 2010
| | Sample_submission_date | Sep 01 2010
| | Sample_last_update_date | Sep 01 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Cells were photoactivated in the dark or light zones of intact lymph nodes and sorted by flow cytometry
| | Sample_growth_protocol_ch1 | Germinal centers were induced by immunization with ovalbumin in alum followed by transfer of PA-GFP+ B1-8hi B cells and boosting with soluble NP-ovalbumin
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared using the Affymetrix 3'IVT Express Kit.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Mouse MOE 430 2.0 gene chips. Chips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | Chips were scanned on Affymetrix GeneChip Scanner 3000 using AGCC 2.0 Software
| | Sample_data_processing | The data were analyzed with GeneSpring 11 using Affymetrix default analysis settings and RMA as normalization method.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Gabriel,D,Victora
| | Sample_contact_email | victora@wi.mit.edu
| | Sample_contact_institute | Whitehead Institute
| | Sample_contact_address | 9 Cambridge Center
| | Sample_contact_city | Cambridge
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02142
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM589nnn/GSM589872/suppl/GSM589872.CEL.gz
| | Sample_series_id | GSE23925
| | Sample_data_row_count | 45101
| | |
|
GSM589873 | GPL1261 |
|
light zone, biological replicate 2
|
day 7 germinal center light zone cells
|
tissue: lymph node
cell type: germinal center B cells
|
Gene expression data from sorted B cells photoactivated in the GC light zone
|
| Sample_geo_accession | GSM589873
| | Sample_status | Public on Sep 07 2010
| | Sample_submission_date | Sep 01 2010
| | Sample_last_update_date | Sep 01 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Cells were photoactivated in the dark or light zones of intact lymph nodes and sorted by flow cytometry
| | Sample_growth_protocol_ch1 | Germinal centers were induced by immunization with ovalbumin in alum followed by transfer of PA-GFP+ B1-8hi B cells and boosting with soluble NP-ovalbumin
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared using the Affymetrix 3'IVT Express Kit.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Mouse MOE 430 2.0 gene chips. Chips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | Chips were scanned on Affymetrix GeneChip Scanner 3000 using AGCC 2.0 Software
| | Sample_data_processing | The data were analyzed with GeneSpring 11 using Affymetrix default analysis settings and RMA as normalization method.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Gabriel,D,Victora
| | Sample_contact_email | victora@wi.mit.edu
| | Sample_contact_institute | Whitehead Institute
| | Sample_contact_address | 9 Cambridge Center
| | Sample_contact_city | Cambridge
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02142
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM589nnn/GSM589873/suppl/GSM589873.CEL.gz
| | Sample_series_id | GSE23925
| | Sample_data_row_count | 45101
| | |
|
GSM589874 | GPL1261 |
|
dark zone, biological replicate 1
|
day 7 germinal center dark zone cells
|
tissue: lymph node
cell type: germinal center B cells
|
Gene expression data from sorted B cells photoactivated in the GC dark zone
|
| Sample_geo_accession | GSM589874
| | Sample_status | Public on Sep 07 2010
| | Sample_submission_date | Sep 01 2010
| | Sample_last_update_date | Sep 01 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Cells were photoactivated in the dark or light zones of intact lymph nodes and sorted by flow cytometry
| | Sample_growth_protocol_ch1 | Germinal centers were induced by immunization with ovalbumin in alum followed by transfer of PA-GFP+ B1-8hi B cells and boosting with soluble NP-ovalbumin
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared using the Affymetrix 3'IVT Express Kit.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Mouse MOE 430 2.0 gene chips. Chips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | Chips were scanned on Affymetrix GeneChip Scanner 3000 using AGCC 2.0 Software
| | Sample_data_processing | The data were analyzed with GeneSpring 11 using Affymetrix default analysis settings and RMA as normalization method.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Gabriel,D,Victora
| | Sample_contact_email | victora@wi.mit.edu
| | Sample_contact_institute | Whitehead Institute
| | Sample_contact_address | 9 Cambridge Center
| | Sample_contact_city | Cambridge
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02142
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM589nnn/GSM589874/suppl/GSM589874.CEL.gz
| | Sample_series_id | GSE23925
| | Sample_data_row_count | 45101
| | |
|
GSM589875 | GPL1261 |
|
dark zone, biological replicate 2
|
day 7 germinal center dark zone cells
|
tissue: lymph node
cell type: germinal center B cells
|
Gene expression data from sorted B cells photoactivated in the GC dark zone
|
| Sample_geo_accession | GSM589875
| | Sample_status | Public on Sep 07 2010
| | Sample_submission_date | Sep 01 2010
| | Sample_last_update_date | Sep 01 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Cells were photoactivated in the dark or light zones of intact lymph nodes and sorted by flow cytometry
| | Sample_growth_protocol_ch1 | Germinal centers were induced by immunization with ovalbumin in alum followed by transfer of PA-GFP+ B1-8hi B cells and boosting with soluble NP-ovalbumin
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared using the Affymetrix 3'IVT Express Kit.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Mouse MOE 430 2.0 gene chips. Chips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | Chips were scanned on Affymetrix GeneChip Scanner 3000 using AGCC 2.0 Software
| | Sample_data_processing | The data were analyzed with GeneSpring 11 using Affymetrix default analysis settings and RMA as normalization method.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Gabriel,D,Victora
| | Sample_contact_email | victora@wi.mit.edu
| | Sample_contact_institute | Whitehead Institute
| | Sample_contact_address | 9 Cambridge Center
| | Sample_contact_city | Cambridge
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02142
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM589nnn/GSM589875/suppl/GSM589875.CEL.gz
| | Sample_series_id | GSE23925
| | Sample_data_row_count | 45101
| | |
|
GSM589876 | GPL1261 |
|
naive, biological replicate 1
|
naïve splenic B cells
|
tissue: spleen
cell type: naive B cells
|
Gene expression data from naïve (IgD+) B cells from spleen
|
| Sample_geo_accession | GSM589876
| | Sample_status | Public on Sep 07 2010
| | Sample_submission_date | Sep 01 2010
| | Sample_last_update_date | Sep 01 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Cells were photoactivated in the dark or light zones of intact lymph nodes and sorted by flow cytometry
| | Sample_growth_protocol_ch1 | Germinal centers were induced by immunization with ovalbumin in alum followed by transfer of PA-GFP+ B1-8hi B cells and boosting with soluble NP-ovalbumin
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared using the Affymetrix 3'IVT Express Kit.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Mouse MOE 430 2.0 gene chips. Chips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | Chips were scanned on Affymetrix GeneChip Scanner 3000 using AGCC 2.0 Software
| | Sample_data_processing | The data were analyzed with GeneSpring 11 using Affymetrix default analysis settings and RMA as normalization method.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Gabriel,D,Victora
| | Sample_contact_email | victora@wi.mit.edu
| | Sample_contact_institute | Whitehead Institute
| | Sample_contact_address | 9 Cambridge Center
| | Sample_contact_city | Cambridge
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02142
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM589nnn/GSM589876/suppl/GSM589876.CEL.gz
| | Sample_series_id | GSE23925
| | Sample_data_row_count | 45101
| | |
|
GSM589877 | GPL1261 |
|
naive, biological replicate 2
|
naïve splenic B cells
|
tissue: spleen
cell type: naive B cells
|
Gene expression data from naïve (IgD+) B cells from spleen
|
| Sample_geo_accession | GSM589877
| | Sample_status | Public on Sep 07 2010
| | Sample_submission_date | Sep 01 2010
| | Sample_last_update_date | Sep 01 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Cells were photoactivated in the dark or light zones of intact lymph nodes and sorted by flow cytometry
| | Sample_growth_protocol_ch1 | Germinal centers were induced by immunization with ovalbumin in alum followed by transfer of PA-GFP+ B1-8hi B cells and boosting with soluble NP-ovalbumin
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared using the Affymetrix 3'IVT Express Kit.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Mouse MOE 430 2.0 gene chips. Chips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | Chips were scanned on Affymetrix GeneChip Scanner 3000 using AGCC 2.0 Software
| | Sample_data_processing | The data were analyzed with GeneSpring 11 using Affymetrix default analysis settings and RMA as normalization method.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Gabriel,D,Victora
| | Sample_contact_email | victora@wi.mit.edu
| | Sample_contact_institute | Whitehead Institute
| | Sample_contact_address | 9 Cambridge Center
| | Sample_contact_city | Cambridge
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02142
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM589nnn/GSM589877/suppl/GSM589877.CEL.gz
| | Sample_series_id | GSE23925
| | Sample_data_row_count | 45101
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