Search results for the GEO ID: GSE24026  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM591440 | GPL3921 |
|
Jurkat-PD1_3-28-PD1_rep1
|
PD1-expressing Jurkat cells incubated for 18h with PD-1/CD3/CD38 beads
|
cell type: Jurkat cells
|
Gene expression data from Jurkat cells cultured with beads
|
| Sample_geo_accession | GSM591440
| | Sample_status | Public on Oct 03 2010
| | Sample_submission_date | Sep 08 2010
| | Sample_last_update_date | Sep 16 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | PD-1 expressing Jurkat cells were cultured for 18 hours with beads coated with antibodies to CD3 and CD28, with our without an antibody to PD-1.
| | Sample_growth_protocol_ch1 | PD-1 expressing Jurkat cells were harvested in mid-log phase of growth.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA extraction was performed using the RNAdvance Tissue Isolation kit (Agencourt). Concentrations of total RNA were determined using a Nanodrop spectrophotometer or via the Ribogreen RNA quantitation kit (Molecular Probes/Invitrogen). RNA purity was determined by Bioanalyzer 2100 traces (Agilent Technologies).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | cDNA was labeled using the Nugen FL-Ovation cDNA Biotin Module V2 kit, following the manufacturer's protocol.
| | Sample_hyb_protocol | Hybridization and washing was performed on the Affymetrix GeneChip Array Station (GCAS) automation platform.
| | Sample_scan_protocol | The arrays were scanned on the GeneChip HT Array Plate Scanner (Affymetrix 00-0332).
| | Sample_data_processing | The data were processed using GenePattern (RMA), RenderCat (enrichment tests), and custom scripts (differential expression, collapsing genes to probes, etc).
| | Sample_platform_id | GPL3921
| | Sample_contact_name | W.,Nicholas,Haining
| | Sample_contact_laboratory | Haining lab
| | Sample_contact_department | Pediatric Oncology
| | Sample_contact_institute | Dana-Farber Cancer Institute
| | Sample_contact_address | 44 Binney Street
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02115
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM591nnn/GSM591440/suppl/GSM591440.CEL.gz
| | Sample_series_id | GSE24026
| | Sample_series_id | GSE24082
| | Sample_data_row_count | 22277
| | |
|
GSM591441 | GPL3921 |
|
Jurkat-PD1_3-28-PD1_rep2
|
PD1-expressing Jurkat cells incubated for 18h with PD-1/CD3/CD38 beads
|
cell type: Jurkat cells
|
Gene expression data from Jurkat cells cultured with beads
|
| Sample_geo_accession | GSM591441
| | Sample_status | Public on Oct 03 2010
| | Sample_submission_date | Sep 08 2010
| | Sample_last_update_date | Sep 16 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | PD-1 expressing Jurkat cells were cultured for 18 hours with beads coated with antibodies to CD3 and CD28, with our without an antibody to PD-1.
| | Sample_growth_protocol_ch1 | PD-1 expressing Jurkat cells were harvested in mid-log phase of growth.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA extraction was performed using the RNAdvance Tissue Isolation kit (Agencourt). Concentrations of total RNA were determined using a Nanodrop spectrophotometer or via the Ribogreen RNA quantitation kit (Molecular Probes/Invitrogen). RNA purity was determined by Bioanalyzer 2100 traces (Agilent Technologies).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | cDNA was labeled using the Nugen FL-Ovation cDNA Biotin Module V2 kit, following the manufacturer's protocol.
| | Sample_hyb_protocol | Hybridization and washing was performed on the Affymetrix GeneChip Array Station (GCAS) automation platform.
| | Sample_scan_protocol | The arrays were scanned on the GeneChip HT Array Plate Scanner (Affymetrix 00-0332).
| | Sample_data_processing | The data were processed using GenePattern (RMA), RenderCat (enrichment tests), and custom scripts (differential expression, collapsing genes to probes, etc).
| | Sample_platform_id | GPL3921
| | Sample_contact_name | W.,Nicholas,Haining
| | Sample_contact_laboratory | Haining lab
| | Sample_contact_department | Pediatric Oncology
| | Sample_contact_institute | Dana-Farber Cancer Institute
| | Sample_contact_address | 44 Binney Street
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02115
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM591nnn/GSM591441/suppl/GSM591441.CEL.gz
| | Sample_series_id | GSE24026
| | Sample_series_id | GSE24082
| | Sample_data_row_count | 22277
| | |
|
GSM591442 | GPL3921 |
|
Jurkat-PD1_3-28-PD1_rep3
|
PD1-expressing Jurkat cells incubated for 18h with PD-1/CD3/CD38 beads
|
cell type: Jurkat cells
|
Gene expression data from Jurkat cells cultured with beads
|
| Sample_geo_accession | GSM591442
| | Sample_status | Public on Oct 03 2010
| | Sample_submission_date | Sep 08 2010
| | Sample_last_update_date | Sep 16 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | PD-1 expressing Jurkat cells were cultured for 18 hours with beads coated with antibodies to CD3 and CD28, with our without an antibody to PD-1.
| | Sample_growth_protocol_ch1 | PD-1 expressing Jurkat cells were harvested in mid-log phase of growth.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA extraction was performed using the RNAdvance Tissue Isolation kit (Agencourt). Concentrations of total RNA were determined using a Nanodrop spectrophotometer or via the Ribogreen RNA quantitation kit (Molecular Probes/Invitrogen). RNA purity was determined by Bioanalyzer 2100 traces (Agilent Technologies).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | cDNA was labeled using the Nugen FL-Ovation cDNA Biotin Module V2 kit, following the manufacturer's protocol.
| | Sample_hyb_protocol | Hybridization and washing was performed on the Affymetrix GeneChip Array Station (GCAS) automation platform.
| | Sample_scan_protocol | The arrays were scanned on the GeneChip HT Array Plate Scanner (Affymetrix 00-0332).
| | Sample_data_processing | The data were processed using GenePattern (RMA), RenderCat (enrichment tests), and custom scripts (differential expression, collapsing genes to probes, etc).
| | Sample_platform_id | GPL3921
| | Sample_contact_name | W.,Nicholas,Haining
| | Sample_contact_laboratory | Haining lab
| | Sample_contact_department | Pediatric Oncology
| | Sample_contact_institute | Dana-Farber Cancer Institute
| | Sample_contact_address | 44 Binney Street
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02115
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM591nnn/GSM591442/suppl/GSM591442.CEL.gz
| | Sample_series_id | GSE24026
| | Sample_series_id | GSE24082
| | Sample_data_row_count | 22277
| | |
|
GSM591443 | GPL3921 |
|
Jurkat-PD1_3-28-PD1_rep4
|
PD1-expressing Jurkat cells incubated for 18h with PD-1/CD3/CD38 beads
|
cell type: Jurkat cells
|
Gene expression data from Jurkat cells cultured with beads
|
| Sample_geo_accession | GSM591443
| | Sample_status | Public on Oct 03 2010
| | Sample_submission_date | Sep 08 2010
| | Sample_last_update_date | Sep 16 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | PD-1 expressing Jurkat cells were cultured for 18 hours with beads coated with antibodies to CD3 and CD28, with our without an antibody to PD-1.
| | Sample_growth_protocol_ch1 | PD-1 expressing Jurkat cells were harvested in mid-log phase of growth.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA extraction was performed using the RNAdvance Tissue Isolation kit (Agencourt). Concentrations of total RNA were determined using a Nanodrop spectrophotometer or via the Ribogreen RNA quantitation kit (Molecular Probes/Invitrogen). RNA purity was determined by Bioanalyzer 2100 traces (Agilent Technologies).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | cDNA was labeled using the Nugen FL-Ovation cDNA Biotin Module V2 kit, following the manufacturer's protocol.
| | Sample_hyb_protocol | Hybridization and washing was performed on the Affymetrix GeneChip Array Station (GCAS) automation platform.
| | Sample_scan_protocol | The arrays were scanned on the GeneChip HT Array Plate Scanner (Affymetrix 00-0332).
| | Sample_data_processing | The data were processed using GenePattern (RMA), RenderCat (enrichment tests), and custom scripts (differential expression, collapsing genes to probes, etc).
| | Sample_platform_id | GPL3921
| | Sample_contact_name | W.,Nicholas,Haining
| | Sample_contact_laboratory | Haining lab
| | Sample_contact_department | Pediatric Oncology
| | Sample_contact_institute | Dana-Farber Cancer Institute
| | Sample_contact_address | 44 Binney Street
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02115
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM591nnn/GSM591443/suppl/GSM591443.CEL.gz
| | Sample_series_id | GSE24026
| | Sample_series_id | GSE24082
| | Sample_data_row_count | 22277
| | |
|
GSM591444 | GPL3921 |
|
Jurkat-PD1_3-28-PD1_rep5
|
PD1-expressing Jurkat cells incubated for 18h with PD-1/CD3/CD38 beads
|
cell type: Jurkat cells
|
Gene expression data from Jurkat cells cultured with beads
|
| Sample_geo_accession | GSM591444
| | Sample_status | Public on Oct 03 2010
| | Sample_submission_date | Sep 08 2010
| | Sample_last_update_date | Sep 16 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | PD-1 expressing Jurkat cells were cultured for 18 hours with beads coated with antibodies to CD3 and CD28, with our without an antibody to PD-1.
| | Sample_growth_protocol_ch1 | PD-1 expressing Jurkat cells were harvested in mid-log phase of growth.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA extraction was performed using the RNAdvance Tissue Isolation kit (Agencourt). Concentrations of total RNA were determined using a Nanodrop spectrophotometer or via the Ribogreen RNA quantitation kit (Molecular Probes/Invitrogen). RNA purity was determined by Bioanalyzer 2100 traces (Agilent Technologies).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | cDNA was labeled using the Nugen FL-Ovation cDNA Biotin Module V2 kit, following the manufacturer's protocol.
| | Sample_hyb_protocol | Hybridization and washing was performed on the Affymetrix GeneChip Array Station (GCAS) automation platform.
| | Sample_scan_protocol | The arrays were scanned on the GeneChip HT Array Plate Scanner (Affymetrix 00-0332).
| | Sample_data_processing | The data were processed using GenePattern (RMA), RenderCat (enrichment tests), and custom scripts (differential expression, collapsing genes to probes, etc).
| | Sample_platform_id | GPL3921
| | Sample_contact_name | W.,Nicholas,Haining
| | Sample_contact_laboratory | Haining lab
| | Sample_contact_department | Pediatric Oncology
| | Sample_contact_institute | Dana-Farber Cancer Institute
| | Sample_contact_address | 44 Binney Street
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02115
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM591nnn/GSM591444/suppl/GSM591444.CEL.gz
| | Sample_series_id | GSE24026
| | Sample_series_id | GSE24082
| | Sample_data_row_count | 22277
| | |
|
GSM591445 | GPL3921 |
|
Jurkat-PD1_3-28-PD1_rep6
|
PD1-expressing Jurkat cells incubated for 18h with PD-1/CD3/CD38 beads
|
cell type: Jurkat cells
|
Gene expression data from Jurkat cells cultured with beads
|
| Sample_geo_accession | GSM591445
| | Sample_status | Public on Oct 03 2010
| | Sample_submission_date | Sep 08 2010
| | Sample_last_update_date | Sep 16 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | PD-1 expressing Jurkat cells were cultured for 18 hours with beads coated with antibodies to CD3 and CD28, with our without an antibody to PD-1.
| | Sample_growth_protocol_ch1 | PD-1 expressing Jurkat cells were harvested in mid-log phase of growth.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA extraction was performed using the RNAdvance Tissue Isolation kit (Agencourt). Concentrations of total RNA were determined using a Nanodrop spectrophotometer or via the Ribogreen RNA quantitation kit (Molecular Probes/Invitrogen). RNA purity was determined by Bioanalyzer 2100 traces (Agilent Technologies).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | cDNA was labeled using the Nugen FL-Ovation cDNA Biotin Module V2 kit, following the manufacturer's protocol.
| | Sample_hyb_protocol | Hybridization and washing was performed on the Affymetrix GeneChip Array Station (GCAS) automation platform.
| | Sample_scan_protocol | The arrays were scanned on the GeneChip HT Array Plate Scanner (Affymetrix 00-0332).
| | Sample_data_processing | The data were processed using GenePattern (RMA), RenderCat (enrichment tests), and custom scripts (differential expression, collapsing genes to probes, etc).
| | Sample_platform_id | GPL3921
| | Sample_contact_name | W.,Nicholas,Haining
| | Sample_contact_laboratory | Haining lab
| | Sample_contact_department | Pediatric Oncology
| | Sample_contact_institute | Dana-Farber Cancer Institute
| | Sample_contact_address | 44 Binney Street
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02115
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM591nnn/GSM591445/suppl/GSM591445.CEL.gz
| | Sample_series_id | GSE24026
| | Sample_series_id | GSE24082
| | Sample_data_row_count | 22277
| | |
|
GSM591446 | GPL3921 |
|
Jurkat-PD1_3-28_rep1
|
PD1-expressing Jurkat cells incubated for 18h with CD3/CD38 beads
|
cell type: Jurkat cells
|
Gene expression data from Jurkat cells cultured with beads
|
| Sample_geo_accession | GSM591446
| | Sample_status | Public on Oct 03 2010
| | Sample_submission_date | Sep 08 2010
| | Sample_last_update_date | Sep 16 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | PD-1 expressing Jurkat cells were cultured for 18 hours with beads coated with antibodies to CD3 and CD28, with our without an antibody to PD-1.
| | Sample_growth_protocol_ch1 | PD-1 expressing Jurkat cells were harvested in mid-log phase of growth.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA extraction was performed using the RNAdvance Tissue Isolation kit (Agencourt). Concentrations of total RNA were determined using a Nanodrop spectrophotometer or via the Ribogreen RNA quantitation kit (Molecular Probes/Invitrogen). RNA purity was determined by Bioanalyzer 2100 traces (Agilent Technologies).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | cDNA was labeled using the Nugen FL-Ovation cDNA Biotin Module V2 kit, following the manufacturer's protocol.
| | Sample_hyb_protocol | Hybridization and washing was performed on the Affymetrix GeneChip Array Station (GCAS) automation platform.
| | Sample_scan_protocol | The arrays were scanned on the GeneChip HT Array Plate Scanner (Affymetrix 00-0332).
| | Sample_data_processing | The data were processed using GenePattern (RMA), RenderCat (enrichment tests), and custom scripts (differential expression, collapsing genes to probes, etc).
| | Sample_platform_id | GPL3921
| | Sample_contact_name | W.,Nicholas,Haining
| | Sample_contact_laboratory | Haining lab
| | Sample_contact_department | Pediatric Oncology
| | Sample_contact_institute | Dana-Farber Cancer Institute
| | Sample_contact_address | 44 Binney Street
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02115
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM591nnn/GSM591446/suppl/GSM591446.CEL.gz
| | Sample_series_id | GSE24026
| | Sample_series_id | GSE24082
| | Sample_data_row_count | 22277
| | |
|
GSM591447 | GPL3921 |
|
Jurkat-PD1_3-28_rep3
|
PD1-expressing Jurkat cells incubated for 18h with CD3/CD38 beads
|
cell type: Jurkat cells
|
Gene expression data from Jurkat cells cultured with beads
|
| Sample_geo_accession | GSM591447
| | Sample_status | Public on Oct 03 2010
| | Sample_submission_date | Sep 08 2010
| | Sample_last_update_date | Sep 16 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | PD-1 expressing Jurkat cells were cultured for 18 hours with beads coated with antibodies to CD3 and CD28, with our without an antibody to PD-1.
| | Sample_growth_protocol_ch1 | PD-1 expressing Jurkat cells were harvested in mid-log phase of growth.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA extraction was performed using the RNAdvance Tissue Isolation kit (Agencourt). Concentrations of total RNA were determined using a Nanodrop spectrophotometer or via the Ribogreen RNA quantitation kit (Molecular Probes/Invitrogen). RNA purity was determined by Bioanalyzer 2100 traces (Agilent Technologies).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | cDNA was labeled using the Nugen FL-Ovation cDNA Biotin Module V2 kit, following the manufacturer's protocol.
| | Sample_hyb_protocol | Hybridization and washing was performed on the Affymetrix GeneChip Array Station (GCAS) automation platform.
| | Sample_scan_protocol | The arrays were scanned on the GeneChip HT Array Plate Scanner (Affymetrix 00-0332).
| | Sample_data_processing | The data were processed using GenePattern (RMA), RenderCat (enrichment tests), and custom scripts (differential expression, collapsing genes to probes, etc).
| | Sample_platform_id | GPL3921
| | Sample_contact_name | W.,Nicholas,Haining
| | Sample_contact_laboratory | Haining lab
| | Sample_contact_department | Pediatric Oncology
| | Sample_contact_institute | Dana-Farber Cancer Institute
| | Sample_contact_address | 44 Binney Street
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02115
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM591nnn/GSM591447/suppl/GSM591447.CEL.gz
| | Sample_series_id | GSE24026
| | Sample_series_id | GSE24082
| | Sample_data_row_count | 22277
| | |
|
GSM591448 | GPL3921 |
|
Jurkat-PD1_3-28_rep4
|
PD1-expressing Jurkat cells incubated for 18h with CD3/CD38 beads
|
cell type: Jurkat cells
|
Gene expression data from Jurkat cells cultured with beads
|
| Sample_geo_accession | GSM591448
| | Sample_status | Public on Oct 03 2010
| | Sample_submission_date | Sep 08 2010
| | Sample_last_update_date | Sep 16 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | PD-1 expressing Jurkat cells were cultured for 18 hours with beads coated with antibodies to CD3 and CD28, with our without an antibody to PD-1.
| | Sample_growth_protocol_ch1 | PD-1 expressing Jurkat cells were harvested in mid-log phase of growth.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA extraction was performed using the RNAdvance Tissue Isolation kit (Agencourt). Concentrations of total RNA were determined using a Nanodrop spectrophotometer or via the Ribogreen RNA quantitation kit (Molecular Probes/Invitrogen). RNA purity was determined by Bioanalyzer 2100 traces (Agilent Technologies).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | cDNA was labeled using the Nugen FL-Ovation cDNA Biotin Module V2 kit, following the manufacturer's protocol.
| | Sample_hyb_protocol | Hybridization and washing was performed on the Affymetrix GeneChip Array Station (GCAS) automation platform.
| | Sample_scan_protocol | The arrays were scanned on the GeneChip HT Array Plate Scanner (Affymetrix 00-0332).
| | Sample_data_processing | The data were processed using GenePattern (RMA), RenderCat (enrichment tests), and custom scripts (differential expression, collapsing genes to probes, etc).
| | Sample_platform_id | GPL3921
| | Sample_contact_name | W.,Nicholas,Haining
| | Sample_contact_laboratory | Haining lab
| | Sample_contact_department | Pediatric Oncology
| | Sample_contact_institute | Dana-Farber Cancer Institute
| | Sample_contact_address | 44 Binney Street
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02115
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM591nnn/GSM591448/suppl/GSM591448.CEL.gz
| | Sample_series_id | GSE24026
| | Sample_series_id | GSE24082
| | Sample_data_row_count | 22277
| | |
|
GSM591449 | GPL3921 |
|
Jurkat-PD1_3-28_rep5
|
PD1-expressing Jurkat cells incubated for 18h with CD3/CD38 beads
|
cell type: Jurkat cells
|
Gene expression data from Jurkat cells cultured with beads
|
| Sample_geo_accession | GSM591449
| | Sample_status | Public on Oct 03 2010
| | Sample_submission_date | Sep 08 2010
| | Sample_last_update_date | Sep 16 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | PD-1 expressing Jurkat cells were cultured for 18 hours with beads coated with antibodies to CD3 and CD28, with our without an antibody to PD-1.
| | Sample_growth_protocol_ch1 | PD-1 expressing Jurkat cells were harvested in mid-log phase of growth.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA extraction was performed using the RNAdvance Tissue Isolation kit (Agencourt). Concentrations of total RNA were determined using a Nanodrop spectrophotometer or via the Ribogreen RNA quantitation kit (Molecular Probes/Invitrogen). RNA purity was determined by Bioanalyzer 2100 traces (Agilent Technologies).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | cDNA was labeled using the Nugen FL-Ovation cDNA Biotin Module V2 kit, following the manufacturer's protocol.
| | Sample_hyb_protocol | Hybridization and washing was performed on the Affymetrix GeneChip Array Station (GCAS) automation platform.
| | Sample_scan_protocol | The arrays were scanned on the GeneChip HT Array Plate Scanner (Affymetrix 00-0332).
| | Sample_data_processing | The data were processed using GenePattern (RMA), RenderCat (enrichment tests), and custom scripts (differential expression, collapsing genes to probes, etc).
| | Sample_platform_id | GPL3921
| | Sample_contact_name | W.,Nicholas,Haining
| | Sample_contact_laboratory | Haining lab
| | Sample_contact_department | Pediatric Oncology
| | Sample_contact_institute | Dana-Farber Cancer Institute
| | Sample_contact_address | 44 Binney Street
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02115
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM591nnn/GSM591449/suppl/GSM591449.CEL.gz
| | Sample_series_id | GSE24026
| | Sample_series_id | GSE24082
| | Sample_data_row_count | 22277
| | |
|
GSM591450 | GPL3921 |
|
Jurkat-PD1_3-28_rep6
|
PD1-expressing Jurkat cells incubated for 18h with CD3/CD38 beads
|
cell type: Jurkat cells
|
Gene expression data from Jurkat cells cultured with beads
|
| Sample_geo_accession | GSM591450
| | Sample_status | Public on Oct 03 2010
| | Sample_submission_date | Sep 08 2010
| | Sample_last_update_date | Sep 16 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | PD-1 expressing Jurkat cells were cultured for 18 hours with beads coated with antibodies to CD3 and CD28, with our without an antibody to PD-1.
| | Sample_growth_protocol_ch1 | PD-1 expressing Jurkat cells were harvested in mid-log phase of growth.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA extraction was performed using the RNAdvance Tissue Isolation kit (Agencourt). Concentrations of total RNA were determined using a Nanodrop spectrophotometer or via the Ribogreen RNA quantitation kit (Molecular Probes/Invitrogen). RNA purity was determined by Bioanalyzer 2100 traces (Agilent Technologies).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | cDNA was labeled using the Nugen FL-Ovation cDNA Biotin Module V2 kit, following the manufacturer's protocol.
| | Sample_hyb_protocol | Hybridization and washing was performed on the Affymetrix GeneChip Array Station (GCAS) automation platform.
| | Sample_scan_protocol | The arrays were scanned on the GeneChip HT Array Plate Scanner (Affymetrix 00-0332).
| | Sample_data_processing | The data were processed using GenePattern (RMA), RenderCat (enrichment tests), and custom scripts (differential expression, collapsing genes to probes, etc).
| | Sample_platform_id | GPL3921
| | Sample_contact_name | W.,Nicholas,Haining
| | Sample_contact_laboratory | Haining lab
| | Sample_contact_department | Pediatric Oncology
| | Sample_contact_institute | Dana-Farber Cancer Institute
| | Sample_contact_address | 44 Binney Street
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | 02115
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM591nnn/GSM591450/suppl/GSM591450.CEL.gz
| | Sample_series_id | GSE24026
| | Sample_series_id | GSE24082
| | Sample_data_row_count | 22277
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