Search results for the GEO ID: GSE24027  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM591451 | GPL570 |
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VLX_50_MAS5
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Breast cancer cell line MCF7, VLX 50
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cell line: MCF7
cell type: breast cancer cells
perturbagen: small molecule
treatment: VLX 50
concentration: 0.00001 M
vehicle: DMSO
duration: 6 h
sample type: treatment
|
Gene expression data from MCF7 cells treated with VLX 50 for 6 hours.
|
| Sample_geo_accession | GSM591451
| | Sample_status | Public on Sep 09 2011
| | Sample_submission_date | Sep 08 2010
| | Sample_last_update_date | Sep 09 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | MCF7 cells treated with 10 microM VLX 50 for 6 hours.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Extraction of total RNA was performed using the Qiagen Rneasy minikit according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 2 micrograms of total RNA from each sample were used to prepare biotinylated fragmented cRNA according to the GeneChip® Expression Analysis Technical Manual (Rev. 5, Affymetrix Inc., Santa Clara, CA).
| | Sample_hyb_protocol | Affymetrix GeneChip® expression arrays (Human Genome U133 Plus 2.0 Array) were hybridized for 16 hours in a 45°C incubator, rotated at 60 rpm.
| | Sample_scan_protocol | According to the GeneChip® Expression Analysis Technical Manual (Rev. 5, Affymetrix Inc., Santa Clara, CA), the arrays were then washed and stained using the Fluidics Station 450 and finally scanned using the GeneChip® Scanner 3000 7G.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Mårten,,Fryknäs
| | Sample_contact_email | marten.fryknas@medsci.uu.se
| | Sample_contact_laboratory | Clinical pharmacology
| | Sample_contact_department | Medical sciencies
| | Sample_contact_institute | Uppsala university
| | Sample_contact_address | Akademiska sjh ingång 61
| | Sample_contact_city | Uppsala
| | Sample_contact_zip/postal_code | 74185
| | Sample_contact_country | Sweden
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM591nnn/GSM591451/suppl/GSM591451_VLX_50.CEL.gz
| | Sample_series_id | GSE24027
| | Sample_data_row_count | 54675
| | |
|
GSM591452 | GPL570 |
|
DMSO_control_MAS5
|
Breast cancer cell line MCF7, DMSO
|
cell line: MCF7
cell type: breast cancer cells
perturbagen: small molecule
treatment: DMSO
concentration: 0.1%
duration: 6 h
sample type: control
|
Gene expression data from MCF7 cells treated with 0.1% DMSO for 6 hours.
|
| Sample_geo_accession | GSM591452
| | Sample_status | Public on Sep 09 2011
| | Sample_submission_date | Sep 08 2010
| | Sample_last_update_date | Sep 09 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | MCF7 control cells treated with 0.1% DMSO for 6 hours.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Extraction of total RNA was performed using the Qiagen Rneasy minikit according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 2 micrograms of total RNA from each sample were used to prepare biotinylated fragmented cRNA according to the GeneChip® Expression Analysis Technical Manual (Rev. 5, Affymetrix Inc., Santa Clara, CA).
| | Sample_hyb_protocol | Affymetrix GeneChip® expression arrays (Human Genome U133 Plus 2.0 Array) were hybridized for 16 hours in a 45°C incubator, rotated at 60 rpm.
| | Sample_scan_protocol | According to the GeneChip® Expression Analysis Technical Manual (Rev. 5, Affymetrix Inc., Santa Clara, CA), the arrays were then washed and stained using the Fluidics Station 450 and finally scanned using the GeneChip® Scanner 3000 7G.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Mårten,,Fryknäs
| | Sample_contact_email | marten.fryknas@medsci.uu.se
| | Sample_contact_laboratory | Clinical pharmacology
| | Sample_contact_department | Medical sciencies
| | Sample_contact_institute | Uppsala university
| | Sample_contact_address | Akademiska sjh ingång 61
| | Sample_contact_city | Uppsala
| | Sample_contact_zip/postal_code | 74185
| | Sample_contact_country | Sweden
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM591nnn/GSM591452/suppl/GSM591452_DMSO_control.CEL.gz
| | Sample_series_id | GSE24027
| | Sample_data_row_count | 54675
| | |
|
GSM591453 | GPL570 |
|
VLX_50_RMA
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Breast cancer cell line MCF7, VLX 50
|
cell line: MCF7
cell type: breast cancer cells
perturbagen: small molecule
treatment: VLX 50
concentration: 0.00001 M
vehicle: DMSO
duration: 6 h
sample type: treatment
|
Gene expression data from MCF7 cells treated with VLX 50 for 6 hours.
|
| Sample_geo_accession | GSM591453
| | Sample_status | Public on Sep 09 2011
| | Sample_submission_date | Sep 08 2010
| | Sample_last_update_date | Sep 09 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | MCF7 cells treated with 10 microM VLX 50 for 6 hours.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Extraction of total RNA was performed using the Qiagen Rneasy minikit according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 2 micrograms of total RNA from each sample were used to prepare biotinylated fragmented cRNA according to the GeneChip® Expression Analysis Technical Manual (Rev. 5, Affymetrix Inc., Santa Clara, CA).
| | Sample_hyb_protocol | Affymetrix GeneChip® expression arrays (Human Genome U133 Plus 2.0 Array) were hybridized for 16 hours in a 45°C incubator, rotated at 60 rpm.
| | Sample_scan_protocol | According to the GeneChip® Expression Analysis Technical Manual (Rev. 5, Affymetrix Inc., Santa Clara, CA), the arrays were then washed and stained using the Fluidics Station 450 and finally scanned using the GeneChip® Scanner 3000 7G.
| | Sample_data_processing | The data were analyzed using the robust multi-array average (RMA) available from the Bioconductor project in the free statistical computing language R.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Mårten,,Fryknäs
| | Sample_contact_email | marten.fryknas@medsci.uu.se
| | Sample_contact_laboratory | Clinical pharmacology
| | Sample_contact_department | Medical sciencies
| | Sample_contact_institute | Uppsala university
| | Sample_contact_address | Akademiska sjh ingång 61
| | Sample_contact_city | Uppsala
| | Sample_contact_zip/postal_code | 74185
| | Sample_contact_country | Sweden
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM591nnn/GSM591453/suppl/GSM591453_VLX_50.CEL.gz
| | Sample_series_id | GSE24027
| | Sample_data_row_count | 54675
| | |
|
GSM591454 | GPL570 |
|
DMSO_control_RMA
|
Breast cancer cell line MCF7, DMSO
|
cell line: MCF7
cell type: breast cancer cells
perturbagen: small molecule
treatment: DMSO
concentration: 0.1%
duration: 6 h
sample type: control
|
Gene expression data from MCF7 cells treated with 0.1% DMSO for 6 hours.
|
| Sample_geo_accession | GSM591454
| | Sample_status | Public on Sep 09 2011
| | Sample_submission_date | Sep 08 2010
| | Sample_last_update_date | Sep 09 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | MCF7 control cells treated with 0.1% DMSO for 6 hours.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Extraction of total RNA was performed using the Qiagen Rneasy minikit according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 2 micrograms of total RNA from each sample were used to prepare biotinylated fragmented cRNA according to the GeneChip® Expression Analysis Technical Manual (Rev. 5, Affymetrix Inc., Santa Clara, CA).
| | Sample_hyb_protocol | Affymetrix GeneChip® expression arrays (Human Genome U133 Plus 2.0 Array) were hybridized for 16 hours in a 45°C incubator, rotated at 60 rpm.
| | Sample_scan_protocol | According to the GeneChip® Expression Analysis Technical Manual (Rev. 5, Affymetrix Inc., Santa Clara, CA), the arrays were then washed and stained using the Fluidics Station 450 and finally scanned using the GeneChip® Scanner 3000 7G.
| | Sample_data_processing | The data were analyzed using the robust multi-array average (RMA) available from the Bioconductor project in the free statistical computing language R.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Mårten,,Fryknäs
| | Sample_contact_email | marten.fryknas@medsci.uu.se
| | Sample_contact_laboratory | Clinical pharmacology
| | Sample_contact_department | Medical sciencies
| | Sample_contact_institute | Uppsala university
| | Sample_contact_address | Akademiska sjh ingång 61
| | Sample_contact_city | Uppsala
| | Sample_contact_zip/postal_code | 74185
| | Sample_contact_country | Sweden
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM591nnn/GSM591454/suppl/GSM591454_DMSO_control.CEL.gz
| | Sample_series_id | GSE24027
| | Sample_data_row_count | 54675
| | |
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