Search results for the GEO ID: GSE24042 |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM561873 | GPL570 |
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Adult human liver tissue expression replica 1
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pooled RNA sample from ten human-liver RNAs
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tissue: normal liver tissues from patients with liver hemangioma
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none
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Sample_geo_accession | GSM561873
| Sample_status | Public on Sep 18 2010
| Sample_submission_date | Jun 30 2010
| Sample_last_update_date | Sep 17 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Adult human liver tissues from 10 adult human were surgically resected due to hemangioma in the liver. The samples were obtained from the portion unaffected by the hemangioma and were immediately frozen in liquid nitrogen. All procedures and risks were explained verbally, and patients provided written consent. The samples were sectioned and confirmed to be histologically normal. All laboratory data assessing hepatic function were within normal ranges, including serum alanine aminotransferase, aspartate aminotransferase, gamma-glutamyl transpeptidase, alkaline phosphatase, total bilirubin, albumin, prothrombin activity, glucose, cholesterol, and triglycerides (data not shown). Serological tests for hepatitis B surface antigen, hepatitis C virus antibodies, and human immunodeficiency virus antibodies also proved negative. Neither heavy alcohol consumption nor the intake of prescription or other drugs was observed before surgical resection. Total RNA was extracted according to the manufacturer’s instructions, and RNase-free DNase I was used to remove DNA contamination. Nucleic acid concentration and purity were assessed at 260 nm using a spectrophotometer (DU 530, Beckman-Coulter Inc., Fullerton, CA), and the quality was assessed using an Agilent 2100 Bioanalyzer. In order to address gene expression polymorphism, the total RNA from 10 livers was pooled.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | The pooled RNA sample from ten human-liver RNAs in same amount were labeled by biotin
| Sample_hyb_protocol | The pooled RNA sample from ten human-liver RNAs in same amount were hybridized to HG-U133plus 2.0 high-density oligonucleotide arrays (Affymetrix), which contains 54,675 probe sets representing 19164 human genes and 15136 ESTs on a single array.
| Sample_scan_protocol | One ug of poly(A)+ RNA was annealed to oligo(dT) and transcribed using SurperScript II reverse transcriptase (Invitrogen, Carlsbad, CA), Labeling, hybridization, washing and signal scan on the microarrays were performed according to the manufacturer's instructions.
| Sample_data_processing | Primary image analysis of the arrays was performed by using GENECHIP 3.2 (Affymetrix, Santa Clara, CA), and normalization was performed using Mas 5.0 software (Affymetrix).
| Sample_platform_id | GPL570
| Sample_contact_name | yao,,yu
| Sample_contact_institute | sibs
| Sample_contact_address | yueyang road 320, room 123
| Sample_contact_city | shanghai
| Sample_contact_zip/postal_code | 200231
| Sample_contact_country | China
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM561nnn/GSM561873/suppl/GSM561873_NB2010120_2_Normal_liver_1.CEL.gz
| Sample_series_id | GSE24042
| Sample_data_row_count | 54675
| |
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GSM561882 | GPL570 |
|
Adult human liver tissue expression replica 2
|
pooled RNA sample from ten human-liver RNAs
|
tissue: normal liver tissues from patients with liver hemangioma
|
none
|
Sample_geo_accession | GSM561882
| Sample_status | Public on Sep 18 2010
| Sample_submission_date | Jun 30 2010
| Sample_last_update_date | Sep 17 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Adult human liver tissues from 10 adult human were surgically resected due to hemangioma in the liver. The samples were obtained from the portion unaffected by the hemangioma and were immediately frozen in liquid nitrogen. All procedures and risks were explained verbally, and patients provided written consent. The samples were sectioned and confirmed to be histologically normal. All laboratory data assessing hepatic function were within normal ranges, including serum alanine aminotransferase, aspartate aminotransferase, gamma-glutamyl transpeptidase, alkaline phosphatase, total bilirubin, albumin, prothrombin activity, glucose, cholesterol, and triglycerides (data not shown). Serological tests for hepatitis B surface antigen, hepatitis C virus antibodies, and human immunodeficiency virus antibodies also proved negative. Neither heavy alcohol consumption nor the intake of prescription or other drugs was observed before surgical resection. Total RNA was extracted according to the manufacturer’s instructions, and RNase-free DNase I was used to remove DNA contamination. Nucleic acid concentration and purity were assessed at 260 nm using a spectrophotometer (DU 530, Beckman-Coulter Inc., Fullerton, CA), and the quality was assessed using an Agilent 2100 Bioanalyzer. In order to address gene expression polymorphism, the total RNA from 10 livers was pooled.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | The pooled RNA sample from ten human-liver RNAs in same amount were labeled by biotin
| Sample_hyb_protocol | The pooled RNA sample from ten human-liver RNAs in same amount were hybridized to HG-U133plus 2.0 high-density oligonucleotide arrays (Affymetrix), which contains 54,675 probe sets representing 19164 human genes and 15136 ESTs on a single array.
| Sample_scan_protocol | One ug of poly(A)+ RNA was annealed to oligo(dT) and transcribed using SurperScript II reverse transcriptase (Invitrogen, Carlsbad, CA), Labeling, hybridization, washing and signal scan on the microarrays were performed according to the manufacturer's instructions.
| Sample_data_processing | Primary image analysis of the arrays was performed by using GENECHIP 3.2 (Affymetrix, Santa Clara, CA), and normalization was performed using Mas 5.0 software (Affymetrix).
| Sample_platform_id | GPL570
| Sample_contact_name | yao,,yu
| Sample_contact_institute | sibs
| Sample_contact_address | yueyang road 320, room 123
| Sample_contact_city | shanghai
| Sample_contact_zip/postal_code | 200231
| Sample_contact_country | China
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM561nnn/GSM561882/suppl/GSM561882_NB2010120_2_Normal_liver_2.CEL.gz
| Sample_series_id | GSE24042
| Sample_data_row_count | 54675
| |
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