Search results for the GEO ID: GSE24044 |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM591694 | GPL570 |
|
GC B cell_PCMV_rep1
|
GC B cell, control vector
|
tissue: tonsils
cell type: CD10+ GC B cells
developmental stage: pediatric
transfection: vector
|
Total RNA from MACS-sorted GC B cells.
|
Sample_geo_accession | GSM591694
| Sample_status | Public on Jul 27 2011
| Sample_submission_date | Sep 09 2010
| Sample_last_update_date | Jul 27 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_biomaterial_provider_ch1 | Children's hospital, Birmingham; Institute for Cancer Studies, Birmingham
| Sample_treatment_protocol_ch1 | Mononuclear cells were isolated by Ficoll-Isopaque centrifugation of tonsils from pediatric patients under informed consent and with ethical approval. Briefly, CD10+ GC B cells were isolated by magnetic separation at 4oC with anti-CD10-Phycoerythrin (PE) and anti-PE microbeads with LS columns (Miltenyi Biotec).
| Sample_growth_protocol_ch1 | Cells were snap frozen after isolation.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total cellular RNA was isolated using RNeasy Mini Kit (Qiagen)
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Affymetrix labelling protocol
| Sample_hyb_protocol | 10ug of fragmented cRNA was hybridized onto Human Genome U133 Plus 2.0 Array using Affymetrix hybridization protocol.
| Sample_scan_protocol | Affymetrix Standard protocol
| Sample_data_processing | GCOS
| Sample_platform_id | GPL570
| Sample_contact_name | Wenbin,,Wei
| Sample_contact_email | w.wei@bham.ac.uk
| Sample_contact_phone | +44-121-4143293
| Sample_contact_laboratory | Bioinformatics
| Sample_contact_department | School of Cancer Sciences
| Sample_contact_institute | University of Birmingham
| Sample_contact_address | Vincent Drive
| Sample_contact_city | Edgbaston
| Sample_contact_state | West Midlands
| Sample_contact_zip/postal_code | B15 2TT
| Sample_contact_country | United Kingdom
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM591nnn/GSM591694/suppl/GSM591694.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM591nnn/GSM591694/suppl/GSM591694.CHP.gz
| Sample_series_id | GSE24044
| Sample_data_row_count | 54675
| |
|
GSM591695 | GPL570 |
|
GC B cell_PCMV_rep2
|
GC B cell, control vector
|
tissue: tonsils
cell type: CD10+ GC B cells
developmental stage: pediatric
transfection: vector
|
Total RNA from MACS-sorted GC B cells.
|
Sample_geo_accession | GSM591695
| Sample_status | Public on Jul 27 2011
| Sample_submission_date | Sep 09 2010
| Sample_last_update_date | Jul 27 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_biomaterial_provider_ch1 | Children's hospital, Birmingham; Institute for Cancer Studies, Birmingham
| Sample_treatment_protocol_ch1 | Mononuclear cells were isolated by Ficoll-Isopaque centrifugation of tonsils from pediatric patients under informed consent and with ethical approval. Briefly, CD10+ GC B cells were isolated by magnetic separation at 4oC with anti-CD10-Phycoerythrin (PE) and anti-PE microbeads with LS columns (Miltenyi Biotec).
| Sample_growth_protocol_ch1 | Cells were snap frozen after isolation.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total cellular RNA was isolated using RNeasy Mini Kit (Qiagen)
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Affymetrix labelling protocol
| Sample_hyb_protocol | 10ug of fragmented cRNA was hybridized onto Human Genome U133 Plus 2.0 Array using Affymetrix hybridization protocol.
| Sample_scan_protocol | Affymetrix Standard protocol
| Sample_data_processing | GCOS
| Sample_platform_id | GPL570
| Sample_contact_name | Wenbin,,Wei
| Sample_contact_email | w.wei@bham.ac.uk
| Sample_contact_phone | +44-121-4143293
| Sample_contact_laboratory | Bioinformatics
| Sample_contact_department | School of Cancer Sciences
| Sample_contact_institute | University of Birmingham
| Sample_contact_address | Vincent Drive
| Sample_contact_city | Edgbaston
| Sample_contact_state | West Midlands
| Sample_contact_zip/postal_code | B15 2TT
| Sample_contact_country | United Kingdom
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM591nnn/GSM591695/suppl/GSM591695.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM591nnn/GSM591695/suppl/GSM591695.CHP.gz
| Sample_series_id | GSE24044
| Sample_data_row_count | 54675
| |
|
GSM591696 | GPL570 |
|
GC B cell_PCMV_rep3
|
GC B cell, control vector
|
tissue: tonsils
cell type: CD10+ GC B cells
developmental stage: pediatric
transfection: vector
|
Total RNA from MACS-sorted GC B cells.
|
Sample_geo_accession | GSM591696
| Sample_status | Public on Jul 27 2011
| Sample_submission_date | Sep 09 2010
| Sample_last_update_date | Jul 27 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_biomaterial_provider_ch1 | Children's hospital, Birmingham; Institute for Cancer Studies, Birmingham
| Sample_treatment_protocol_ch1 | Mononuclear cells were isolated by Ficoll-Isopaque centrifugation of tonsils from pediatric patients under informed consent and with ethical approval. Briefly, CD10+ GC B cells were isolated by magnetic separation at 4oC with anti-CD10-Phycoerythrin (PE) and anti-PE microbeads with LS columns (Miltenyi Biotec).
| Sample_growth_protocol_ch1 | Cells were snap frozen after isolation.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total cellular RNA was isolated using RNeasy Mini Kit (Qiagen)
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Affymetrix labelling protocol
| Sample_hyb_protocol | 10ug of fragmented cRNA was hybridized onto Human Genome U133 Plus 2.0 Array using Affymetrix hybridization protocol.
| Sample_scan_protocol | Affymetrix Standard protocol
| Sample_data_processing | GCOS
| Sample_platform_id | GPL570
| Sample_contact_name | Wenbin,,Wei
| Sample_contact_email | w.wei@bham.ac.uk
| Sample_contact_phone | +44-121-4143293
| Sample_contact_laboratory | Bioinformatics
| Sample_contact_department | School of Cancer Sciences
| Sample_contact_institute | University of Birmingham
| Sample_contact_address | Vincent Drive
| Sample_contact_city | Edgbaston
| Sample_contact_state | West Midlands
| Sample_contact_zip/postal_code | B15 2TT
| Sample_contact_country | United Kingdom
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM591nnn/GSM591696/suppl/GSM591696.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM591nnn/GSM591696/suppl/GSM591696.CHP.gz
| Sample_series_id | GSE24044
| Sample_data_row_count | 54675
| |
|
GSM591697 | GPL570 |
|
GC B cell_KDM6B_rep1
|
GC B cell, KDM6B
|
tissue: tonsils
cell type: CD10+ GC B cells
developmental stage: pediatric
transfection: KDM6B
|
Total RNA from MACS-sorted GC B cells.
|
Sample_geo_accession | GSM591697
| Sample_status | Public on Jul 27 2011
| Sample_submission_date | Sep 09 2010
| Sample_last_update_date | Jul 27 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_biomaterial_provider_ch1 | Children's hospital, Birmingham; Institute for Cancer Studies, Birmingham
| Sample_treatment_protocol_ch1 | Mononuclear cells were isolated by Ficoll-Isopaque centrifugation of tonsils from pediatric patients under informed consent and with ethical approval. Briefly, CD10+ GC B cells were isolated by magnetic separation at 4oC with anti-CD10-Phycoerythrin (PE) and anti-PE microbeads with LS columns (Miltenyi Biotec).
| Sample_growth_protocol_ch1 | Cells were snap frozen after isolation.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total cellular RNA was isolated using RNeasy Mini Kit (Qiagen)
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Affymetrix labelling protocol
| Sample_hyb_protocol | 10ug of fragmented cRNA was hybridized onto Human Genome U133 Plus 2.0 Array using Affymetrix hybridization protocol.
| Sample_scan_protocol | Affymetrix Standard protocol
| Sample_data_processing | GCOS
| Sample_platform_id | GPL570
| Sample_contact_name | Wenbin,,Wei
| Sample_contact_email | w.wei@bham.ac.uk
| Sample_contact_phone | +44-121-4143293
| Sample_contact_laboratory | Bioinformatics
| Sample_contact_department | School of Cancer Sciences
| Sample_contact_institute | University of Birmingham
| Sample_contact_address | Vincent Drive
| Sample_contact_city | Edgbaston
| Sample_contact_state | West Midlands
| Sample_contact_zip/postal_code | B15 2TT
| Sample_contact_country | United Kingdom
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM591nnn/GSM591697/suppl/GSM591697.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM591nnn/GSM591697/suppl/GSM591697.CHP.gz
| Sample_series_id | GSE24044
| Sample_data_row_count | 54675
| |
|
GSM591698 | GPL570 |
|
GC B cell_KDM6B_rep2
|
GC B cell, KDM6B
|
tissue: tonsils
cell type: CD10+ GC B cells
developmental stage: pediatric
transfection: KDM6B
|
Total RNA from MACS-sorted GC B cells.
|
Sample_geo_accession | GSM591698
| Sample_status | Public on Jul 27 2011
| Sample_submission_date | Sep 09 2010
| Sample_last_update_date | Jul 27 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_biomaterial_provider_ch1 | Children's hospital, Birmingham; Institute for Cancer Studies, Birmingham
| Sample_treatment_protocol_ch1 | Mononuclear cells were isolated by Ficoll-Isopaque centrifugation of tonsils from pediatric patients under informed consent and with ethical approval. Briefly, CD10+ GC B cells were isolated by magnetic separation at 4oC with anti-CD10-Phycoerythrin (PE) and anti-PE microbeads with LS columns (Miltenyi Biotec).
| Sample_growth_protocol_ch1 | Cells were snap frozen after isolation.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total cellular RNA was isolated using RNeasy Mini Kit (Qiagen)
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Affymetrix labelling protocol
| Sample_hyb_protocol | 10ug of fragmented cRNA was hybridized onto Human Genome U133 Plus 2.0 Array using Affymetrix hybridization protocol.
| Sample_scan_protocol | Affymetrix Standard protocol
| Sample_data_processing | GCOS
| Sample_platform_id | GPL570
| Sample_contact_name | Wenbin,,Wei
| Sample_contact_email | w.wei@bham.ac.uk
| Sample_contact_phone | +44-121-4143293
| Sample_contact_laboratory | Bioinformatics
| Sample_contact_department | School of Cancer Sciences
| Sample_contact_institute | University of Birmingham
| Sample_contact_address | Vincent Drive
| Sample_contact_city | Edgbaston
| Sample_contact_state | West Midlands
| Sample_contact_zip/postal_code | B15 2TT
| Sample_contact_country | United Kingdom
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM591nnn/GSM591698/suppl/GSM591698.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM591nnn/GSM591698/suppl/GSM591698.CHP.gz
| Sample_series_id | GSE24044
| Sample_data_row_count | 54675
| |
|
GSM591699 | GPL570 |
|
GC B cell_KDM6B_rep3
|
GC B cell, KDM6B
|
tissue: tonsils
cell type: CD10+ GC B cells
developmental stage: pediatric
transfection: KDM6B
|
Total RNA from MACS-sorted GC B cells.
|
Sample_geo_accession | GSM591699
| Sample_status | Public on Jul 27 2011
| Sample_submission_date | Sep 09 2010
| Sample_last_update_date | Jul 27 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_biomaterial_provider_ch1 | Children's hospital, Birmingham; Institute for Cancer Studies, Birmingham
| Sample_treatment_protocol_ch1 | Mononuclear cells were isolated by Ficoll-Isopaque centrifugation of tonsils from pediatric patients under informed consent and with ethical approval. Briefly, CD10+ GC B cells were isolated by magnetic separation at 4oC with anti-CD10-Phycoerythrin (PE) and anti-PE microbeads with LS columns (Miltenyi Biotec).
| Sample_growth_protocol_ch1 | Cells were snap frozen after isolation.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total cellular RNA was isolated using RNeasy Mini Kit (Qiagen)
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Affymetrix labelling protocol
| Sample_hyb_protocol | 10ug of fragmented cRNA was hybridized onto Human Genome U133 Plus 2.0 Array using Affymetrix hybridization protocol.
| Sample_scan_protocol | Affymetrix Standard protocol
| Sample_data_processing | GCOS
| Sample_platform_id | GPL570
| Sample_contact_name | Wenbin,,Wei
| Sample_contact_email | w.wei@bham.ac.uk
| Sample_contact_phone | +44-121-4143293
| Sample_contact_laboratory | Bioinformatics
| Sample_contact_department | School of Cancer Sciences
| Sample_contact_institute | University of Birmingham
| Sample_contact_address | Vincent Drive
| Sample_contact_city | Edgbaston
| Sample_contact_state | West Midlands
| Sample_contact_zip/postal_code | B15 2TT
| Sample_contact_country | United Kingdom
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM591nnn/GSM591699/suppl/GSM591699.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM591nnn/GSM591699/suppl/GSM591699.CHP.gz
| Sample_series_id | GSE24044
| Sample_data_row_count | 54675
| |
|
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