Search results for the GEO ID: GSE24047  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM591739 | GPL1355 |
|
brain_at 3h after trauma_rep1
|
lateral cortex, traumatic brain injury
|
strain: Wistar
gender: male
age: 8 weeks old
tissue: brain, lateral cortex
stress: moderate fluid percussion injury
time: 3h
|
impacted region
3(M-5)
|
| Sample_geo_accession | GSM591739
| | Sample_status | Public on Dec 07 2010
| | Sample_submission_date | Sep 09 2010
| | Sample_last_update_date | Dec 07 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | Rats were transcardially perfused with physiological saline under general anesthesia at 3, 6, 12 and 48 h after moderate fluid percussion or the sham operation. The brains were quickly removed and cut coronally into 2-mm-thick sections. The cortices that corresponded to the contusion areas were removed. They were then placed in RNAlaterTM (Takara, Japan) overnight at 4°C and stored at –20 °C until use.
| | Sample_growth_protocol_ch1 | Rats were performed craniectomy of diameter 4.8 mm over the right parietal cortex under general anesthesia. A cranial Leur adapter of inner diameter 2.5 mm was placed on the craniectomy site and mounted to the skull by using dental acrylic resin. The animals were housed for 48 h, and they were then re-anesthetized. The cranial Leur adapter was filled with saline and attached to the fluid percussion device. Animals were subjected to sham or moderate (3.3 ± 0.3 atm) fluid percussions.
| | Sample_molecule_ch1 | polyA RNA
| | Sample_extract_protocol_ch1 | Polyadenylated RNA was isolated using the Quickprep micro mRNA purification kit (Amersham, USA) according to the manufacturer’s protocol.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the Affymetrix protocol from 0.4 ug PolyA RNA (One-cycle Target Labeling: GeneChip Expression Analysis Technical Manual, 701021 Rev. 5).
| | Sample_hyb_protocol | cRNA were hybridized on GeneChip rat genome 230 2.0 using the Hybridization Oven 640 110V. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 (Affymetrix 00-0074).
| | Sample_data_processing | Data were pre-processed by RMA method, filtered, and normalized using the GeneSpring GX v11.0.2 software (Agilent). Compared data from experimental conditions are normalized versus array's base line median considered for each case.
| | Sample_platform_id | GPL1355
| | Sample_contact_name | Hideki,,Shojo
| | Sample_contact_email | hshohjoh@yamanashi.ac.jp
| | Sample_contact_phone | 81-55-273-9546
| | Sample_contact_fax | 81-55-273-6753
| | Sample_contact_laboratory | Department of Legal Medicine
| | Sample_contact_department | Graduate School of Medical Science
| | Sample_contact_institute | University of Yamanashi
| | Sample_contact_address | 1110 shimokato
| | Sample_contact_city | Chuo
| | Sample_contact_state | Yamanashi
| | Sample_contact_zip/postal_code | 409-3898
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM591nnn/GSM591739/suppl/GSM591739.CEL.gz
| | Sample_series_id | GSE24047
| | Sample_data_row_count | 31099
| | |
|
GSM591740 | GPL1355 |
|
brain_at 3h after trauma_rep2
|
lateral cortex, traumatic brain injury
|
strain: Wistar
gender: male
age: 8 weeks old
tissue: brain, lateral cortex
stress: moderate fluid percussion injury
time: 3h
|
impacted region
3(M-17)
|
| Sample_geo_accession | GSM591740
| | Sample_status | Public on Dec 07 2010
| | Sample_submission_date | Sep 09 2010
| | Sample_last_update_date | Dec 07 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | Rats were transcardially perfused with physiological saline under general anesthesia at 3, 6, 12 and 48 h after moderate fluid percussion or the sham operation. The brains were quickly removed and cut coronally into 2-mm-thick sections. The cortices that corresponded to the contusion areas were removed. They were then placed in RNAlaterTM (Takara, Japan) overnight at 4°C and stored at –20 °C until use.
| | Sample_growth_protocol_ch1 | Rats were performed craniectomy of diameter 4.8 mm over the right parietal cortex under general anesthesia. A cranial Leur adapter of inner diameter 2.5 mm was placed on the craniectomy site and mounted to the skull by using dental acrylic resin. The animals were housed for 48 h, and they were then re-anesthetized. The cranial Leur adapter was filled with saline and attached to the fluid percussion device. Animals were subjected to sham or moderate (3.3 ± 0.3 atm) fluid percussions.
| | Sample_molecule_ch1 | polyA RNA
| | Sample_extract_protocol_ch1 | Polyadenylated RNA was isolated using the Quickprep micro mRNA purification kit (Amersham, USA) according to the manufacturer’s protocol.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the Affymetrix protocol from 0.4 ug PolyA RNA (One-cycle Target Labeling: GeneChip Expression Analysis Technical Manual, 701021 Rev. 5).
| | Sample_hyb_protocol | cRNA were hybridized on GeneChip rat genome 230 2.0 using the Hybridization Oven 640 110V. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 (Affymetrix 00-0074).
| | Sample_data_processing | Data were pre-processed by RMA method, filtered, and normalized using the GeneSpring GX v11.0.2 software (Agilent). Compared data from experimental conditions are normalized versus array's base line median considered for each case.
| | Sample_platform_id | GPL1355
| | Sample_contact_name | Hideki,,Shojo
| | Sample_contact_email | hshohjoh@yamanashi.ac.jp
| | Sample_contact_phone | 81-55-273-9546
| | Sample_contact_fax | 81-55-273-6753
| | Sample_contact_laboratory | Department of Legal Medicine
| | Sample_contact_department | Graduate School of Medical Science
| | Sample_contact_institute | University of Yamanashi
| | Sample_contact_address | 1110 shimokato
| | Sample_contact_city | Chuo
| | Sample_contact_state | Yamanashi
| | Sample_contact_zip/postal_code | 409-3898
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM591nnn/GSM591740/suppl/GSM591740.CEL.gz
| | Sample_series_id | GSE24047
| | Sample_data_row_count | 31099
| | |
|
GSM591741 | GPL1355 |
|
brain_at 3h after trauma_rep3
|
lateral cortex, traumatic brain injury
|
strain: Wistar
gender: male
age: 8 weeks old
tissue: brain, lateral cortex
stress: moderate fluid percussion injury
time: 3h
|
impacted region
3(M-20)
|
| Sample_geo_accession | GSM591741
| | Sample_status | Public on Dec 07 2010
| | Sample_submission_date | Sep 09 2010
| | Sample_last_update_date | Dec 07 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | Rats were transcardially perfused with physiological saline under general anesthesia at 3, 6, 12 and 48 h after moderate fluid percussion or the sham operation. The brains were quickly removed and cut coronally into 2-mm-thick sections. The cortices that corresponded to the contusion areas were removed. They were then placed in RNAlaterTM (Takara, Japan) overnight at 4°C and stored at –20 °C until use.
| | Sample_growth_protocol_ch1 | Rats were performed craniectomy of diameter 4.8 mm over the right parietal cortex under general anesthesia. A cranial Leur adapter of inner diameter 2.5 mm was placed on the craniectomy site and mounted to the skull by using dental acrylic resin. The animals were housed for 48 h, and they were then re-anesthetized. The cranial Leur adapter was filled with saline and attached to the fluid percussion device. Animals were subjected to sham or moderate (3.3 ± 0.3 atm) fluid percussions.
| | Sample_molecule_ch1 | polyA RNA
| | Sample_extract_protocol_ch1 | Polyadenylated RNA was isolated using the Quickprep micro mRNA purification kit (Amersham, USA) according to the manufacturer’s protocol.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the Affymetrix protocol from 0.4 ug PolyA RNA (One-cycle Target Labeling: GeneChip Expression Analysis Technical Manual, 701021 Rev. 5).
| | Sample_hyb_protocol | cRNA were hybridized on GeneChip rat genome 230 2.0 using the Hybridization Oven 640 110V. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 (Affymetrix 00-0074).
| | Sample_data_processing | Data were pre-processed by RMA method, filtered, and normalized using the GeneSpring GX v11.0.2 software (Agilent). Compared data from experimental conditions are normalized versus array's base line median considered for each case.
| | Sample_platform_id | GPL1355
| | Sample_contact_name | Hideki,,Shojo
| | Sample_contact_email | hshohjoh@yamanashi.ac.jp
| | Sample_contact_phone | 81-55-273-9546
| | Sample_contact_fax | 81-55-273-6753
| | Sample_contact_laboratory | Department of Legal Medicine
| | Sample_contact_department | Graduate School of Medical Science
| | Sample_contact_institute | University of Yamanashi
| | Sample_contact_address | 1110 shimokato
| | Sample_contact_city | Chuo
| | Sample_contact_state | Yamanashi
| | Sample_contact_zip/postal_code | 409-3898
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM591nnn/GSM591741/suppl/GSM591741.CEL.gz
| | Sample_series_id | GSE24047
| | Sample_data_row_count | 31099
| | |
|
GSM591742 | GPL1355 |
|
brain_at 6h after trauma_rep1
|
lateral cortex, traumatic brain injury
|
strain: Wistar
gender: male
age: 8 weeks old
tissue: brain, lateral cortex
stress: moderate fluid percussion injury
time: 6h
|
impacted region
6(M-9)
|
| Sample_geo_accession | GSM591742
| | Sample_status | Public on Dec 07 2010
| | Sample_submission_date | Sep 09 2010
| | Sample_last_update_date | Dec 07 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | Rats were transcardially perfused with physiological saline under general anesthesia at 3, 6, 12 and 48 h after moderate fluid percussion or the sham operation. The brains were quickly removed and cut coronally into 2-mm-thick sections. The cortices that corresponded to the contusion areas were removed. They were then placed in RNAlaterTM (Takara, Japan) overnight at 4°C and stored at –20 °C until use.
| | Sample_growth_protocol_ch1 | Rats were performed craniectomy of diameter 4.8 mm over the right parietal cortex under general anesthesia. A cranial Leur adapter of inner diameter 2.5 mm was placed on the craniectomy site and mounted to the skull by using dental acrylic resin. The animals were housed for 48 h, and they were then re-anesthetized. The cranial Leur adapter was filled with saline and attached to the fluid percussion device. Animals were subjected to sham or moderate (3.3 ± 0.3 atm) fluid percussions.
| | Sample_molecule_ch1 | polyA RNA
| | Sample_extract_protocol_ch1 | Polyadenylated RNA was isolated using the Quickprep micro mRNA purification kit (Amersham, USA) according to the manufacturer’s protocol.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the Affymetrix protocol from 0.4 ug PolyA RNA (One-cycle Target Labeling: GeneChip Expression Analysis Technical Manual, 701021 Rev. 5).
| | Sample_hyb_protocol | cRNA were hybridized on GeneChip rat genome 230 2.0 using the Hybridization Oven 640 110V. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 (Affymetrix 00-0074).
| | Sample_data_processing | Data were pre-processed by RMA method, filtered, and normalized using the GeneSpring GX v11.0.2 software (Agilent). Compared data from experimental conditions are normalized versus array's base line median considered for each case.
| | Sample_platform_id | GPL1355
| | Sample_contact_name | Hideki,,Shojo
| | Sample_contact_email | hshohjoh@yamanashi.ac.jp
| | Sample_contact_phone | 81-55-273-9546
| | Sample_contact_fax | 81-55-273-6753
| | Sample_contact_laboratory | Department of Legal Medicine
| | Sample_contact_department | Graduate School of Medical Science
| | Sample_contact_institute | University of Yamanashi
| | Sample_contact_address | 1110 shimokato
| | Sample_contact_city | Chuo
| | Sample_contact_state | Yamanashi
| | Sample_contact_zip/postal_code | 409-3898
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM591nnn/GSM591742/suppl/GSM591742.CEL.gz
| | Sample_series_id | GSE24047
| | Sample_data_row_count | 31099
| | |
|
GSM591743 | GPL1355 |
|
brain_at 6h after trauma_rep2
|
lateral cortex, traumatic brain injury
|
strain: Wistar
gender: male
age: 8 weeks old
tissue: brain, lateral cortex
stress: moderate fluid percussion injury
time: 6h
|
impacted region
6(M-12)
|
| Sample_geo_accession | GSM591743
| | Sample_status | Public on Dec 07 2010
| | Sample_submission_date | Sep 09 2010
| | Sample_last_update_date | Dec 07 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | Rats were transcardially perfused with physiological saline under general anesthesia at 3, 6, 12 and 48 h after moderate fluid percussion or the sham operation. The brains were quickly removed and cut coronally into 2-mm-thick sections. The cortices that corresponded to the contusion areas were removed. They were then placed in RNAlaterTM (Takara, Japan) overnight at 4°C and stored at –20 °C until use.
| | Sample_growth_protocol_ch1 | Rats were performed craniectomy of diameter 4.8 mm over the right parietal cortex under general anesthesia. A cranial Leur adapter of inner diameter 2.5 mm was placed on the craniectomy site and mounted to the skull by using dental acrylic resin. The animals were housed for 48 h, and they were then re-anesthetized. The cranial Leur adapter was filled with saline and attached to the fluid percussion device. Animals were subjected to sham or moderate (3.3 ± 0.3 atm) fluid percussions.
| | Sample_molecule_ch1 | polyA RNA
| | Sample_extract_protocol_ch1 | Polyadenylated RNA was isolated using the Quickprep micro mRNA purification kit (Amersham, USA) according to the manufacturer’s protocol.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the Affymetrix protocol from 0.4 ug PolyA RNA (One-cycle Target Labeling: GeneChip Expression Analysis Technical Manual, 701021 Rev. 5).
| | Sample_hyb_protocol | cRNA were hybridized on GeneChip rat genome 230 2.0 using the Hybridization Oven 640 110V. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 (Affymetrix 00-0074).
| | Sample_data_processing | Data were pre-processed by RMA method, filtered, and normalized using the GeneSpring GX v11.0.2 software (Agilent). Compared data from experimental conditions are normalized versus array's base line median considered for each case.
| | Sample_platform_id | GPL1355
| | Sample_contact_name | Hideki,,Shojo
| | Sample_contact_email | hshohjoh@yamanashi.ac.jp
| | Sample_contact_phone | 81-55-273-9546
| | Sample_contact_fax | 81-55-273-6753
| | Sample_contact_laboratory | Department of Legal Medicine
| | Sample_contact_department | Graduate School of Medical Science
| | Sample_contact_institute | University of Yamanashi
| | Sample_contact_address | 1110 shimokato
| | Sample_contact_city | Chuo
| | Sample_contact_state | Yamanashi
| | Sample_contact_zip/postal_code | 409-3898
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM591nnn/GSM591743/suppl/GSM591743.CEL.gz
| | Sample_series_id | GSE24047
| | Sample_data_row_count | 31099
| | |
|
GSM591744 | GPL1355 |
|
brain_at 6h aftert rauma_rep3
|
lateral cortex, traumatic brain injury
|
strain: Wistar
gender: male
age: 8 weeks old
tissue: brain, lateral cortex
stress: moderate fluid percussion injury
time: 6h
|
impacted region
6(M-18)
|
| Sample_geo_accession | GSM591744
| | Sample_status | Public on Dec 07 2010
| | Sample_submission_date | Sep 09 2010
| | Sample_last_update_date | Dec 07 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | Rats were transcardially perfused with physiological saline under general anesthesia at 3, 6, 12 and 48 h after moderate fluid percussion or the sham operation. The brains were quickly removed and cut coronally into 2-mm-thick sections. The cortices that corresponded to the contusion areas were removed. They were then placed in RNAlaterTM (Takara, Japan) overnight at 4°C and stored at –20 °C until use.
| | Sample_growth_protocol_ch1 | Rats were performed craniectomy of diameter 4.8 mm over the right parietal cortex under general anesthesia. A cranial Leur adapter of inner diameter 2.5 mm was placed on the craniectomy site and mounted to the skull by using dental acrylic resin. The animals were housed for 48 h, and they were then re-anesthetized. The cranial Leur adapter was filled with saline and attached to the fluid percussion device. Animals were subjected to sham or moderate (3.3 ± 0.3 atm) fluid percussions.
| | Sample_molecule_ch1 | polyA RNA
| | Sample_extract_protocol_ch1 | Polyadenylated RNA was isolated using the Quickprep micro mRNA purification kit (Amersham, USA) according to the manufacturer’s protocol.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the Affymetrix protocol from 0.4 ug PolyA RNA (One-cycle Target Labeling: GeneChip Expression Analysis Technical Manual, 701021 Rev. 5).
| | Sample_hyb_protocol | cRNA were hybridized on GeneChip rat genome 230 2.0 using the Hybridization Oven 640 110V. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 (Affymetrix 00-0074).
| | Sample_data_processing | Data were pre-processed by RMA method, filtered, and normalized using the GeneSpring GX v11.0.2 software (Agilent). Compared data from experimental conditions are normalized versus array's base line median considered for each case.
| | Sample_platform_id | GPL1355
| | Sample_contact_name | Hideki,,Shojo
| | Sample_contact_email | hshohjoh@yamanashi.ac.jp
| | Sample_contact_phone | 81-55-273-9546
| | Sample_contact_fax | 81-55-273-6753
| | Sample_contact_laboratory | Department of Legal Medicine
| | Sample_contact_department | Graduate School of Medical Science
| | Sample_contact_institute | University of Yamanashi
| | Sample_contact_address | 1110 shimokato
| | Sample_contact_city | Chuo
| | Sample_contact_state | Yamanashi
| | Sample_contact_zip/postal_code | 409-3898
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM591nnn/GSM591744/suppl/GSM591744.CEL.gz
| | Sample_series_id | GSE24047
| | Sample_data_row_count | 31099
| | |
|
GSM591745 | GPL1355 |
|
brain_at 12h after trauma_rep1
|
lateral cortex, traumatic brain injury
|
strain: Wistar
gender: male
age: 8 weeks old
tissue: brain, lateral cortex
stress: moderate fluid percussion injury
time: 12h
|
impacted region
12(M-13)
|
| Sample_geo_accession | GSM591745
| | Sample_status | Public on Dec 07 2010
| | Sample_submission_date | Sep 09 2010
| | Sample_last_update_date | Dec 07 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | Rats were transcardially perfused with physiological saline under general anesthesia at 3, 6, 12 and 48 h after moderate fluid percussion or the sham operation. The brains were quickly removed and cut coronally into 2-mm-thick sections. The cortices that corresponded to the contusion areas were removed. They were then placed in RNAlaterTM (Takara, Japan) overnight at 4°C and stored at –20 °C until use.
| | Sample_growth_protocol_ch1 | Rats were performed craniectomy of diameter 4.8 mm over the right parietal cortex under general anesthesia. A cranial Leur adapter of inner diameter 2.5 mm was placed on the craniectomy site and mounted to the skull by using dental acrylic resin. The animals were housed for 48 h, and they were then re-anesthetized. The cranial Leur adapter was filled with saline and attached to the fluid percussion device. Animals were subjected to sham or moderate (3.3 ± 0.3 atm) fluid percussions.
| | Sample_molecule_ch1 | polyA RNA
| | Sample_extract_protocol_ch1 | Polyadenylated RNA was isolated using the Quickprep micro mRNA purification kit (Amersham, USA) according to the manufacturer’s protocol.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the Affymetrix protocol from 0.4 ug PolyA RNA (One-cycle Target Labeling: GeneChip Expression Analysis Technical Manual, 701021 Rev. 5).
| | Sample_hyb_protocol | cRNA were hybridized on GeneChip rat genome 230 2.0 using the Hybridization Oven 640 110V. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 (Affymetrix 00-0074).
| | Sample_data_processing | Data were pre-processed by RMA method, filtered, and normalized using the GeneSpring GX v11.0.2 software (Agilent). Compared data from experimental conditions are normalized versus array's base line median considered for each case.
| | Sample_platform_id | GPL1355
| | Sample_contact_name | Hideki,,Shojo
| | Sample_contact_email | hshohjoh@yamanashi.ac.jp
| | Sample_contact_phone | 81-55-273-9546
| | Sample_contact_fax | 81-55-273-6753
| | Sample_contact_laboratory | Department of Legal Medicine
| | Sample_contact_department | Graduate School of Medical Science
| | Sample_contact_institute | University of Yamanashi
| | Sample_contact_address | 1110 shimokato
| | Sample_contact_city | Chuo
| | Sample_contact_state | Yamanashi
| | Sample_contact_zip/postal_code | 409-3898
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM591nnn/GSM591745/suppl/GSM591745.CEL.gz
| | Sample_series_id | GSE24047
| | Sample_data_row_count | 31099
| | |
|
GSM591746 | GPL1355 |
|
brain_at 12h after trauma_rep2
|
lateral cortex, traumatic brain injury
|
strain: Wistar
gender: male
age: 8 weeks old
tissue: brain, lateral cortex
stress: moderate fluid percussion injury
time: 12h
|
impacted region
12(M-14)
|
| Sample_geo_accession | GSM591746
| | Sample_status | Public on Dec 07 2010
| | Sample_submission_date | Sep 09 2010
| | Sample_last_update_date | Dec 07 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | Rats were transcardially perfused with physiological saline under general anesthesia at 3, 6, 12 and 48 h after moderate fluid percussion or the sham operation. The brains were quickly removed and cut coronally into 2-mm-thick sections. The cortices that corresponded to the contusion areas were removed. They were then placed in RNAlaterTM (Takara, Japan) overnight at 4°C and stored at –20 °C until use.
| | Sample_growth_protocol_ch1 | Rats were performed craniectomy of diameter 4.8 mm over the right parietal cortex under general anesthesia. A cranial Leur adapter of inner diameter 2.5 mm was placed on the craniectomy site and mounted to the skull by using dental acrylic resin. The animals were housed for 48 h, and they were then re-anesthetized. The cranial Leur adapter was filled with saline and attached to the fluid percussion device. Animals were subjected to sham or moderate (3.3 ± 0.3 atm) fluid percussions.
| | Sample_molecule_ch1 | polyA RNA
| | Sample_extract_protocol_ch1 | Polyadenylated RNA was isolated using the Quickprep micro mRNA purification kit (Amersham, USA) according to the manufacturer’s protocol.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the Affymetrix protocol from 0.4 ug PolyA RNA (One-cycle Target Labeling: GeneChip Expression Analysis Technical Manual, 701021 Rev. 5).
| | Sample_hyb_protocol | cRNA were hybridized on GeneChip rat genome 230 2.0 using the Hybridization Oven 640 110V. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 (Affymetrix 00-0074).
| | Sample_data_processing | Data were pre-processed by RMA method, filtered, and normalized using the GeneSpring GX v11.0.2 software (Agilent). Compared data from experimental conditions are normalized versus array's base line median considered for each case.
| | Sample_platform_id | GPL1355
| | Sample_contact_name | Hideki,,Shojo
| | Sample_contact_email | hshohjoh@yamanashi.ac.jp
| | Sample_contact_phone | 81-55-273-9546
| | Sample_contact_fax | 81-55-273-6753
| | Sample_contact_laboratory | Department of Legal Medicine
| | Sample_contact_department | Graduate School of Medical Science
| | Sample_contact_institute | University of Yamanashi
| | Sample_contact_address | 1110 shimokato
| | Sample_contact_city | Chuo
| | Sample_contact_state | Yamanashi
| | Sample_contact_zip/postal_code | 409-3898
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM591nnn/GSM591746/suppl/GSM591746.CEL.gz
| | Sample_series_id | GSE24047
| | Sample_data_row_count | 31099
| | |
|
GSM591747 | GPL1355 |
|
brain_at 12h after trauma_rep3
|
lateral cortex, traumatic brain injury
|
strain: Wistar
gender: male
age: 8 weeks old
tissue: brain, lateral cortex
stress: moderate fluid percussion injury
time: 12h
|
impacted region
12(M-26)
|
| Sample_geo_accession | GSM591747
| | Sample_status | Public on Dec 07 2010
| | Sample_submission_date | Sep 09 2010
| | Sample_last_update_date | Dec 07 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | Rats were transcardially perfused with physiological saline under general anesthesia at 3, 6, 12 and 48 h after moderate fluid percussion or the sham operation. The brains were quickly removed and cut coronally into 2-mm-thick sections. The cortices that corresponded to the contusion areas were removed. They were then placed in RNAlaterTM (Takara, Japan) overnight at 4°C and stored at –20 °C until use.
| | Sample_growth_protocol_ch1 | Rats were performed craniectomy of diameter 4.8 mm over the right parietal cortex under general anesthesia. A cranial Leur adapter of inner diameter 2.5 mm was placed on the craniectomy site and mounted to the skull by using dental acrylic resin. The animals were housed for 48 h, and they were then re-anesthetized. The cranial Leur adapter was filled with saline and attached to the fluid percussion device. Animals were subjected to sham or moderate (3.3 ± 0.3 atm) fluid percussions.
| | Sample_molecule_ch1 | polyA RNA
| | Sample_extract_protocol_ch1 | Polyadenylated RNA was isolated using the Quickprep micro mRNA purification kit (Amersham, USA) according to the manufacturer’s protocol.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the Affymetrix protocol from 0.4 ug PolyA RNA (One-cycle Target Labeling: GeneChip Expression Analysis Technical Manual, 701021 Rev. 5).
| | Sample_hyb_protocol | cRNA were hybridized on GeneChip rat genome 230 2.0 using the Hybridization Oven 640 110V. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 (Affymetrix 00-0074).
| | Sample_data_processing | Data were pre-processed by RMA method, filtered, and normalized using the GeneSpring GX v11.0.2 software (Agilent). Compared data from experimental conditions are normalized versus array's base line median considered for each case.
| | Sample_platform_id | GPL1355
| | Sample_contact_name | Hideki,,Shojo
| | Sample_contact_email | hshohjoh@yamanashi.ac.jp
| | Sample_contact_phone | 81-55-273-9546
| | Sample_contact_fax | 81-55-273-6753
| | Sample_contact_laboratory | Department of Legal Medicine
| | Sample_contact_department | Graduate School of Medical Science
| | Sample_contact_institute | University of Yamanashi
| | Sample_contact_address | 1110 shimokato
| | Sample_contact_city | Chuo
| | Sample_contact_state | Yamanashi
| | Sample_contact_zip/postal_code | 409-3898
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM591nnn/GSM591747/suppl/GSM591747.CEL.gz
| | Sample_series_id | GSE24047
| | Sample_data_row_count | 31099
| | |
|
GSM591748 | GPL1355 |
|
brain_at 48h after trauma_rep1
|
lateral cortex, traumatic brain injury
|
strain: Wistar
gender: male
age: 8 weeks old
tissue: brain, lateral cortex
stress: moderate fluid percussion injury
time: 48h
|
impacted region
48(M-3)
|
| Sample_geo_accession | GSM591748
| | Sample_status | Public on Dec 07 2010
| | Sample_submission_date | Sep 09 2010
| | Sample_last_update_date | Dec 07 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | Rats were transcardially perfused with physiological saline under general anesthesia at 3, 6, 12 and 48 h after moderate fluid percussion or the sham operation. The brains were quickly removed and cut coronally into 2-mm-thick sections. The cortices that corresponded to the contusion areas were removed. They were then placed in RNAlaterTM (Takara, Japan) overnight at 4°C and stored at –20 °C until use.
| | Sample_growth_protocol_ch1 | Rats were performed craniectomy of diameter 4.8 mm over the right parietal cortex under general anesthesia. A cranial Leur adapter of inner diameter 2.5 mm was placed on the craniectomy site and mounted to the skull by using dental acrylic resin. The animals were housed for 48 h, and they were then re-anesthetized. The cranial Leur adapter was filled with saline and attached to the fluid percussion device. Animals were subjected to sham or moderate (3.3 ± 0.3 atm) fluid percussions.
| | Sample_molecule_ch1 | polyA RNA
| | Sample_extract_protocol_ch1 | Polyadenylated RNA was isolated using the Quickprep micro mRNA purification kit (Amersham, USA) according to the manufacturer’s protocol.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the Affymetrix protocol from 0.4 ug PolyA RNA (One-cycle Target Labeling: GeneChip Expression Analysis Technical Manual, 701021 Rev. 5).
| | Sample_hyb_protocol | cRNA were hybridized on GeneChip rat genome 230 2.0 using the Hybridization Oven 640 110V. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 (Affymetrix 00-0074).
| | Sample_data_processing | Data were pre-processed by RMA method, filtered, and normalized using the GeneSpring GX v11.0.2 software (Agilent). Compared data from experimental conditions are normalized versus array's base line median considered for each case.
| | Sample_platform_id | GPL1355
| | Sample_contact_name | Hideki,,Shojo
| | Sample_contact_email | hshohjoh@yamanashi.ac.jp
| | Sample_contact_phone | 81-55-273-9546
| | Sample_contact_fax | 81-55-273-6753
| | Sample_contact_laboratory | Department of Legal Medicine
| | Sample_contact_department | Graduate School of Medical Science
| | Sample_contact_institute | University of Yamanashi
| | Sample_contact_address | 1110 shimokato
| | Sample_contact_city | Chuo
| | Sample_contact_state | Yamanashi
| | Sample_contact_zip/postal_code | 409-3898
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM591nnn/GSM591748/suppl/GSM591748.CEL.gz
| | Sample_series_id | GSE24047
| | Sample_data_row_count | 31099
| | |
|
GSM591749 | GPL1355 |
|
brain_at 48h after trauma_rep2
|
lateral cortex, traumatic brain injury
|
strain: Wistar
gender: male
age: 8 weeks old
tissue: brain, lateral cortex
stress: moderate fluid percussion injury
time: 48h
|
impacted region
48(M-23)
|
| Sample_geo_accession | GSM591749
| | Sample_status | Public on Dec 07 2010
| | Sample_submission_date | Sep 09 2010
| | Sample_last_update_date | Dec 07 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | Rats were transcardially perfused with physiological saline under general anesthesia at 3, 6, 12 and 48 h after moderate fluid percussion or the sham operation. The brains were quickly removed and cut coronally into 2-mm-thick sections. The cortices that corresponded to the contusion areas were removed. They were then placed in RNAlaterTM (Takara, Japan) overnight at 4°C and stored at –20 °C until use.
| | Sample_growth_protocol_ch1 | Rats were performed craniectomy of diameter 4.8 mm over the right parietal cortex under general anesthesia. A cranial Leur adapter of inner diameter 2.5 mm was placed on the craniectomy site and mounted to the skull by using dental acrylic resin. The animals were housed for 48 h, and they were then re-anesthetized. The cranial Leur adapter was filled with saline and attached to the fluid percussion device. Animals were subjected to sham or moderate (3.3 ± 0.3 atm) fluid percussions.
| | Sample_molecule_ch1 | polyA RNA
| | Sample_extract_protocol_ch1 | Polyadenylated RNA was isolated using the Quickprep micro mRNA purification kit (Amersham, USA) according to the manufacturer’s protocol.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the Affymetrix protocol from 0.4 ug PolyA RNA (One-cycle Target Labeling: GeneChip Expression Analysis Technical Manual, 701021 Rev. 5).
| | Sample_hyb_protocol | cRNA were hybridized on GeneChip rat genome 230 2.0 using the Hybridization Oven 640 110V. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 (Affymetrix 00-0074).
| | Sample_data_processing | Data were pre-processed by RMA method, filtered, and normalized using the GeneSpring GX v11.0.2 software (Agilent). Compared data from experimental conditions are normalized versus array's base line median considered for each case.
| | Sample_platform_id | GPL1355
| | Sample_contact_name | Hideki,,Shojo
| | Sample_contact_email | hshohjoh@yamanashi.ac.jp
| | Sample_contact_phone | 81-55-273-9546
| | Sample_contact_fax | 81-55-273-6753
| | Sample_contact_laboratory | Department of Legal Medicine
| | Sample_contact_department | Graduate School of Medical Science
| | Sample_contact_institute | University of Yamanashi
| | Sample_contact_address | 1110 shimokato
| | Sample_contact_city | Chuo
| | Sample_contact_state | Yamanashi
| | Sample_contact_zip/postal_code | 409-3898
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM591nnn/GSM591749/suppl/GSM591749.CEL.gz
| | Sample_series_id | GSE24047
| | Sample_data_row_count | 31099
| | |
|
GSM591750 | GPL1355 |
|
brain_at 48h after trauma_rep3
|
lateral cortex, traumatic brain injury
|
strain: Wistar
gender: male
age: 8 weeks old
tissue: brain, lateral cortex
stress: moderate fluid percussion injury
time: 48h
|
impacted region
48(M-24)
|
| Sample_geo_accession | GSM591750
| | Sample_status | Public on Dec 07 2010
| | Sample_submission_date | Sep 09 2010
| | Sample_last_update_date | Dec 07 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | Rats were transcardially perfused with physiological saline under general anesthesia at 3, 6, 12 and 48 h after moderate fluid percussion or the sham operation. The brains were quickly removed and cut coronally into 2-mm-thick sections. The cortices that corresponded to the contusion areas were removed. They were then placed in RNAlaterTM (Takara, Japan) overnight at 4°C and stored at –20 °C until use.
| | Sample_growth_protocol_ch1 | Rats were performed craniectomy of diameter 4.8 mm over the right parietal cortex under general anesthesia. A cranial Leur adapter of inner diameter 2.5 mm was placed on the craniectomy site and mounted to the skull by using dental acrylic resin. The animals were housed for 48 h, and they were then re-anesthetized. The cranial Leur adapter was filled with saline and attached to the fluid percussion device. Animals were subjected to sham or moderate (3.3 ± 0.3 atm) fluid percussions.
| | Sample_molecule_ch1 | polyA RNA
| | Sample_extract_protocol_ch1 | Polyadenylated RNA was isolated using the Quickprep micro mRNA purification kit (Amersham, USA) according to the manufacturer’s protocol.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the Affymetrix protocol from 0.4 ug PolyA RNA (One-cycle Target Labeling: GeneChip Expression Analysis Technical Manual, 701021 Rev. 5).
| | Sample_hyb_protocol | cRNA were hybridized on GeneChip rat genome 230 2.0 using the Hybridization Oven 640 110V. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 (Affymetrix 00-0074).
| | Sample_data_processing | Data were pre-processed by RMA method, filtered, and normalized using the GeneSpring GX v11.0.2 software (Agilent). Compared data from experimental conditions are normalized versus array's base line median considered for each case.
| | Sample_platform_id | GPL1355
| | Sample_contact_name | Hideki,,Shojo
| | Sample_contact_email | hshohjoh@yamanashi.ac.jp
| | Sample_contact_phone | 81-55-273-9546
| | Sample_contact_fax | 81-55-273-6753
| | Sample_contact_laboratory | Department of Legal Medicine
| | Sample_contact_department | Graduate School of Medical Science
| | Sample_contact_institute | University of Yamanashi
| | Sample_contact_address | 1110 shimokato
| | Sample_contact_city | Chuo
| | Sample_contact_state | Yamanashi
| | Sample_contact_zip/postal_code | 409-3898
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM591nnn/GSM591750/suppl/GSM591750.CEL.gz
| | Sample_series_id | GSE24047
| | Sample_data_row_count | 31099
| | |
|
GSM591751 | GPL1355 |
|
brain_sham_3h_rep1
|
lateral cortex, non-injured control
|
strain: Wistar
gender: male
age: 8 weeks old
tissue: brain, lateral cortex
stress: non-injured control
time: 3h
|
non-injured control
3-sham(M-28)
|
| Sample_geo_accession | GSM591751
| | Sample_status | Public on Dec 07 2010
| | Sample_submission_date | Sep 09 2010
| | Sample_last_update_date | Dec 07 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | Rats were transcardially perfused with physiological saline under general anesthesia at 3, 6, 12 and 48 h after moderate fluid percussion or the sham operation. The brains were quickly removed and cut coronally into 2-mm-thick sections. The cortices that corresponded to the contusion areas were removed. They were then placed in RNAlaterTM (Takara, Japan) overnight at 4°C and stored at –20 °C until use.
| | Sample_growth_protocol_ch1 | Rats were performed craniectomy of diameter 4.8 mm over the right parietal cortex under general anesthesia. A cranial Leur adapter of inner diameter 2.5 mm was placed on the craniectomy site and mounted to the skull by using dental acrylic resin. The animals were housed for 48 h, and they were then re-anesthetized. The cranial Leur adapter was filled with saline and attached to the fluid percussion device. Animals were subjected to sham or moderate (3.3 ± 0.3 atm) fluid percussions.
| | Sample_molecule_ch1 | polyA RNA
| | Sample_extract_protocol_ch1 | Polyadenylated RNA was isolated using the Quickprep micro mRNA purification kit (Amersham, USA) according to the manufacturer’s protocol.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the Affymetrix protocol from 0.4 ug PolyA RNA (One-cycle Target Labeling: GeneChip Expression Analysis Technical Manual, 701021 Rev. 5).
| | Sample_hyb_protocol | cRNA were hybridized on GeneChip rat genome 230 2.0 using the Hybridization Oven 640 110V. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 (Affymetrix 00-0074).
| | Sample_data_processing | Data were pre-processed by RMA method, filtered, and normalized using the GeneSpring GX v11.0.2 software (Agilent). Compared data from experimental conditions are normalized versus array's base line median considered for each case.
| | Sample_platform_id | GPL1355
| | Sample_contact_name | Hideki,,Shojo
| | Sample_contact_email | hshohjoh@yamanashi.ac.jp
| | Sample_contact_phone | 81-55-273-9546
| | Sample_contact_fax | 81-55-273-6753
| | Sample_contact_laboratory | Department of Legal Medicine
| | Sample_contact_department | Graduate School of Medical Science
| | Sample_contact_institute | University of Yamanashi
| | Sample_contact_address | 1110 shimokato
| | Sample_contact_city | Chuo
| | Sample_contact_state | Yamanashi
| | Sample_contact_zip/postal_code | 409-3898
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM591nnn/GSM591751/suppl/GSM591751.CEL.gz
| | Sample_series_id | GSE24047
| | Sample_data_row_count | 31099
| | |
|
GSM591752 | GPL1355 |
|
brain_sham_6h_rep1
|
lateral cortex, non-injured control
|
strain: Wistar
gender: male
age: 8 weeks old
tissue: brain, lateral cortex
stress: non-injured control
time: 6h
|
non-injured control
6-sham(M-21)
|
| Sample_geo_accession | GSM591752
| | Sample_status | Public on Dec 07 2010
| | Sample_submission_date | Sep 09 2010
| | Sample_last_update_date | Dec 07 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | Rats were transcardially perfused with physiological saline under general anesthesia at 3, 6, 12 and 48 h after moderate fluid percussion or the sham operation. The brains were quickly removed and cut coronally into 2-mm-thick sections. The cortices that corresponded to the contusion areas were removed. They were then placed in RNAlaterTM (Takara, Japan) overnight at 4°C and stored at –20 °C until use.
| | Sample_growth_protocol_ch1 | Rats were performed craniectomy of diameter 4.8 mm over the right parietal cortex under general anesthesia. A cranial Leur adapter of inner diameter 2.5 mm was placed on the craniectomy site and mounted to the skull by using dental acrylic resin. The animals were housed for 48 h, and they were then re-anesthetized. The cranial Leur adapter was filled with saline and attached to the fluid percussion device. Animals were subjected to sham or moderate (3.3 ± 0.3 atm) fluid percussions.
| | Sample_molecule_ch1 | polyA RNA
| | Sample_extract_protocol_ch1 | Polyadenylated RNA was isolated using the Quickprep micro mRNA purification kit (Amersham, USA) according to the manufacturer’s protocol.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the Affymetrix protocol from 0.4 ug PolyA RNA (One-cycle Target Labeling: GeneChip Expression Analysis Technical Manual, 701021 Rev. 5).
| | Sample_hyb_protocol | cRNA were hybridized on GeneChip rat genome 230 2.0 using the Hybridization Oven 640 110V. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 (Affymetrix 00-0074).
| | Sample_data_processing | Data were pre-processed by RMA method, filtered, and normalized using the GeneSpring GX v11.0.2 software (Agilent). Compared data from experimental conditions are normalized versus array's base line median considered for each case.
| | Sample_platform_id | GPL1355
| | Sample_contact_name | Hideki,,Shojo
| | Sample_contact_email | hshohjoh@yamanashi.ac.jp
| | Sample_contact_phone | 81-55-273-9546
| | Sample_contact_fax | 81-55-273-6753
| | Sample_contact_laboratory | Department of Legal Medicine
| | Sample_contact_department | Graduate School of Medical Science
| | Sample_contact_institute | University of Yamanashi
| | Sample_contact_address | 1110 shimokato
| | Sample_contact_city | Chuo
| | Sample_contact_state | Yamanashi
| | Sample_contact_zip/postal_code | 409-3898
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM591nnn/GSM591752/suppl/GSM591752.CEL.gz
| | Sample_series_id | GSE24047
| | Sample_data_row_count | 31099
| | |
|
GSM591753 | GPL1355 |
|
brain_sham_12h_rep1
|
lateral cortex, non-injured control
|
strain: Wistar
gender: male
age: 8 weeks old
tissue: brain, lateral cortex
stress: non-injured control
time: 12h
|
non-injured control
12-sham(M-27)
|
| Sample_geo_accession | GSM591753
| | Sample_status | Public on Dec 07 2010
| | Sample_submission_date | Sep 09 2010
| | Sample_last_update_date | Dec 07 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | Rats were transcardially perfused with physiological saline under general anesthesia at 3, 6, 12 and 48 h after moderate fluid percussion or the sham operation. The brains were quickly removed and cut coronally into 2-mm-thick sections. The cortices that corresponded to the contusion areas were removed. They were then placed in RNAlaterTM (Takara, Japan) overnight at 4°C and stored at –20 °C until use.
| | Sample_growth_protocol_ch1 | Rats were performed craniectomy of diameter 4.8 mm over the right parietal cortex under general anesthesia. A cranial Leur adapter of inner diameter 2.5 mm was placed on the craniectomy site and mounted to the skull by using dental acrylic resin. The animals were housed for 48 h, and they were then re-anesthetized. The cranial Leur adapter was filled with saline and attached to the fluid percussion device. Animals were subjected to sham or moderate (3.3 ± 0.3 atm) fluid percussions.
| | Sample_molecule_ch1 | polyA RNA
| | Sample_extract_protocol_ch1 | Polyadenylated RNA was isolated using the Quickprep micro mRNA purification kit (Amersham, USA) according to the manufacturer’s protocol.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the Affymetrix protocol from 0.4 ug PolyA RNA (One-cycle Target Labeling: GeneChip Expression Analysis Technical Manual, 701021 Rev. 5).
| | Sample_hyb_protocol | cRNA were hybridized on GeneChip rat genome 230 2.0 using the Hybridization Oven 640 110V. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 (Affymetrix 00-0074).
| | Sample_data_processing | Data were pre-processed by RMA method, filtered, and normalized using the GeneSpring GX v11.0.2 software (Agilent). Compared data from experimental conditions are normalized versus array's base line median considered for each case.
| | Sample_platform_id | GPL1355
| | Sample_contact_name | Hideki,,Shojo
| | Sample_contact_email | hshohjoh@yamanashi.ac.jp
| | Sample_contact_phone | 81-55-273-9546
| | Sample_contact_fax | 81-55-273-6753
| | Sample_contact_laboratory | Department of Legal Medicine
| | Sample_contact_department | Graduate School of Medical Science
| | Sample_contact_institute | University of Yamanashi
| | Sample_contact_address | 1110 shimokato
| | Sample_contact_city | Chuo
| | Sample_contact_state | Yamanashi
| | Sample_contact_zip/postal_code | 409-3898
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM591nnn/GSM591753/suppl/GSM591753.CEL.gz
| | Sample_series_id | GSE24047
| | Sample_data_row_count | 31099
| | |
|
GSM591754 | GPL1355 |
|
brain_sham_48h_rep1
|
lateral cortex, non-injured control
|
strain: Wistar
gender: male
age: 8 weeks old
tissue: brain, lateral cortex
stress: non-injured control
time: 48h
|
non-injured control
48-sham(M-25)
|
| Sample_geo_accession | GSM591754
| | Sample_status | Public on Dec 07 2010
| | Sample_submission_date | Sep 09 2010
| | Sample_last_update_date | Dec 07 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | Rats were transcardially perfused with physiological saline under general anesthesia at 3, 6, 12 and 48 h after moderate fluid percussion or the sham operation. The brains were quickly removed and cut coronally into 2-mm-thick sections. The cortices that corresponded to the contusion areas were removed. They were then placed in RNAlaterTM (Takara, Japan) overnight at 4°C and stored at –20 °C until use.
| | Sample_growth_protocol_ch1 | Rats were performed craniectomy of diameter 4.8 mm over the right parietal cortex under general anesthesia. A cranial Leur adapter of inner diameter 2.5 mm was placed on the craniectomy site and mounted to the skull by using dental acrylic resin. The animals were housed for 48 h, and they were then re-anesthetized. The cranial Leur adapter was filled with saline and attached to the fluid percussion device. Animals were subjected to sham or moderate (3.3 ± 0.3 atm) fluid percussions.
| | Sample_molecule_ch1 | polyA RNA
| | Sample_extract_protocol_ch1 | Polyadenylated RNA was isolated using the Quickprep micro mRNA purification kit (Amersham, USA) according to the manufacturer’s protocol.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the Affymetrix protocol from 0.4 ug PolyA RNA (One-cycle Target Labeling: GeneChip Expression Analysis Technical Manual, 701021 Rev. 5).
| | Sample_hyb_protocol | cRNA were hybridized on GeneChip rat genome 230 2.0 using the Hybridization Oven 640 110V. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 (Affymetrix 00-0074).
| | Sample_data_processing | Data were pre-processed by RMA method, filtered, and normalized using the GeneSpring GX v11.0.2 software (Agilent). Compared data from experimental conditions are normalized versus array's base line median considered for each case.
| | Sample_platform_id | GPL1355
| | Sample_contact_name | Hideki,,Shojo
| | Sample_contact_email | hshohjoh@yamanashi.ac.jp
| | Sample_contact_phone | 81-55-273-9546
| | Sample_contact_fax | 81-55-273-6753
| | Sample_contact_laboratory | Department of Legal Medicine
| | Sample_contact_department | Graduate School of Medical Science
| | Sample_contact_institute | University of Yamanashi
| | Sample_contact_address | 1110 shimokato
| | Sample_contact_city | Chuo
| | Sample_contact_state | Yamanashi
| | Sample_contact_zip/postal_code | 409-3898
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM591nnn/GSM591754/suppl/GSM591754.CEL.gz
| | Sample_series_id | GSE24047
| | Sample_data_row_count | 31099
| | |
|
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Make groups for comparisons |
(2 groups will be compared at a time) |
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| Select GSMs and click on "Add groups" |
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