Search results for the GEO ID: GSE24150 |
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GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM594398 | GPL570 |
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b-AP15
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Breast cancer cell line MCF7
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treatment: b-AP15
cell line: MCF7
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Gene expression data from MCF7 cells treated with b-AP15 for 6 hours.
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Sample_geo_accession | GSM594398
| Sample_status | Public on Oct 17 2011
| Sample_submission_date | Sep 15 2010
| Sample_last_update_date | Oct 18 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | MCF7 cells treated with 1 microM b-AP15 for 6 hours.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Extraction of total RNA was performed using Qiagen Rneasy minikit according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | 2 micrograms of total RNA from each sample were used to prepare biotinylated fragmented cRNA according to the GeneChip® Expression Analysis Technical Manual (Rev. 5, Affymetrix Inc., Santa Clara, CA).
| Sample_hyb_protocol | Affymetrix GeneChip® expression arrays (Human Genome U133 Plus 2.0 Array) were hybridized for 16 hours in a 45°C incubator, rotated at 60 rpm.
| Sample_scan_protocol | According to the GeneChip® Expression Analysis Technical Manual (Rev. 5, Affymetrix Inc., Santa Clara, CA) the arrays were then washed and stained using the Fluidics Station 450 and finally scanned using the GeneChip® Scanner 3000 7G.
| Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings.
| Sample_platform_id | GPL570
| Sample_contact_name | Mårten,,Fryknäs
| Sample_contact_email | marten.fryknas@medsci.uu.se
| Sample_contact_laboratory | Clinical pharmacology
| Sample_contact_department | Medical sciencies
| Sample_contact_institute | Uppsala university
| Sample_contact_address | Akademiska sjh ingång 61
| Sample_contact_city | Uppsala
| Sample_contact_zip/postal_code | 74185
| Sample_contact_country | Sweden
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM594nnn/GSM594398/suppl/GSM594398_b-AP15.CEL.gz
| Sample_series_id | GSE24150
| Sample_data_row_count | 54675
| |
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GSM594399 | GPL570 |
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DMSO_control
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Breast cancer cell line MCF7
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treatment: DMSO
cell line: MCF7
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Gene expression data from MCF7 cells treated 0.1% DMSO for 6 hours.
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Sample_geo_accession | GSM594399
| Sample_status | Public on Oct 17 2011
| Sample_submission_date | Sep 15 2010
| Sample_last_update_date | Oct 18 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | MCF7 control cells treated with 0.1% DMSO for 6 hours.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Extraction of total RNA was performed using Qiagen Rneasy minikit according to the manufacturer's instructions
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | 2 micrograms of total RNA from each sample were used to prepare biotinylated fragmented cRNA according to the GeneChip® Expression Analysis Technical Manual (Rev. 5, Affymetrix Inc., Santa Clara, CA).
| Sample_hyb_protocol | Affymetrix GeneChip® expression arrays (Human Genome U133 Plus 2.0 Array) were hybridized for 16 hours in a 45°C incubator, rotated at 60 rpm.
| Sample_scan_protocol | According to the GeneChip® Expression Analysis Technical Manual (Rev. 5, Affymetrix Inc., Santa Clara, CA) the arrays were then washed and stained using the Fluidics Station 450 and finally scanned using the GeneChip® Scanner 3000 7G.
| Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings.
| Sample_platform_id | GPL570
| Sample_contact_name | Mårten,,Fryknäs
| Sample_contact_email | marten.fryknas@medsci.uu.se
| Sample_contact_laboratory | Clinical pharmacology
| Sample_contact_department | Medical sciencies
| Sample_contact_institute | Uppsala university
| Sample_contact_address | Akademiska sjh ingång 61
| Sample_contact_city | Uppsala
| Sample_contact_zip/postal_code | 74185
| Sample_contact_country | Sweden
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM594nnn/GSM594399/suppl/GSM594399_DMSO_control.CEL.gz
| Sample_series_id | GSE24150
| Sample_data_row_count | 54675
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