Search results for the GEO ID: GSE24155  |
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GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM594452 | GPL10925 |
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B8_AIM2 positive
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transfected HCT116 cells with persistent AIM2 expression
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cell line: HCT116
sub-clone: B8
aim2 expression: persistent expression
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Gene expression data from transfected HCT116 cells, subclone B8, showing persistent AIM2 expression
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| Sample_geo_accession | GSM594452
| | Sample_status | Public on Dec 13 2012
| | Sample_submission_date | Sep 15 2010
| | Sample_last_update_date | Dec 13 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Construction of subclones D1 and B8 and control was described in Patsos et al., 2009.
| | Sample_growth_protocol_ch1 | All cell lines were grown in RPMI 1640 (Invitrogen, Life Technologies, Karlsruhe, Germany) supplemented with 10% FCS, 100 U/ml penicillin and 100 mg/ml streptomycin.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted using RNeasy Kit (Qiagen, Hilden, Germany) according to the manufacturer’s protocol.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Human Genome 133 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner3000.
| | Sample_data_processing | a CustomCDF file was used to annoatate the microarrays ( http://brainarray.mbni.med.umich.edu/Brainarray/Database/CustomCDF/genomic_curated_CDF.asp, version 10). The data were quantile normalized and analyzed with JMP Genomics based on mixed model ANOVA.
| | Sample_platform_id | GPL10925
| | Sample_contact_name | Li,,Li
| | Sample_contact_email | li.li@gmx.de
| | Sample_contact_institute | University Heidelberg
| | Sample_contact_address | Theodor-Kutzer-Ufer
| | Sample_contact_city | Mannheim
| | Sample_contact_zip/postal_code | 68167
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM594nnn/GSM594452/suppl/GSM594452.CEL.gz
| | Sample_series_id | GSE24155
| | Sample_data_row_count | 22244
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GSM594453 | GPL10925 |
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D1_AIM2 positive
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transfected HCT116 cells with persistent AIM2 expression
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cell line: HCT116
sub-clone: D1
aim2 expression: persistent expression
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Gene expression data from transfected HCT116 cells, subclone D1, showing persistent AIM2 expression
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| Sample_geo_accession | GSM594453
| | Sample_status | Public on Dec 13 2012
| | Sample_submission_date | Sep 15 2010
| | Sample_last_update_date | Dec 13 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Construction of subclones D1 and B8 and control was described in Patsos et al., 2009.
| | Sample_growth_protocol_ch1 | All cell lines were grown in RPMI 1640 (Invitrogen, Life Technologies, Karlsruhe, Germany) supplemented with 10% FCS, 100 U/ml penicillin and 100 mg/ml streptomycin.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted using RNeasy Kit (Qiagen, Hilden, Germany) according to the manufacturer’s protocol.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Human Genome 133 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner3000.
| | Sample_data_processing | a CustomCDF file was used to annoatate the microarrays ( http://brainarray.mbni.med.umich.edu/Brainarray/Database/CustomCDF/genomic_curated_CDF.asp, version 10). The data were quantile normalized and analyzed with JMP Genomics based on mixed model ANOVA.
| | Sample_platform_id | GPL10925
| | Sample_contact_name | Li,,Li
| | Sample_contact_email | li.li@gmx.de
| | Sample_contact_institute | University Heidelberg
| | Sample_contact_address | Theodor-Kutzer-Ufer
| | Sample_contact_city | Mannheim
| | Sample_contact_zip/postal_code | 68167
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM594nnn/GSM594453/suppl/GSM594453.CEL.gz
| | Sample_series_id | GSE24155
| | Sample_data_row_count | 22244
| | |
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GSM594454 | GPL10925 |
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control_AIM2 negative
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mock-transfected HCT116 cells showing absent AIM2 expression
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cell line: HCT116
aim2 expression: absent expression
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Gene expression data from mock-transfected HCT116 cells, showing absent AIM2 expression
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| Sample_geo_accession | GSM594454
| | Sample_status | Public on Dec 13 2012
| | Sample_submission_date | Sep 15 2010
| | Sample_last_update_date | Dec 13 2012
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Construction of subclones D1 and B8 and control was described in Patsos et al., 2009.
| | Sample_growth_protocol_ch1 | All cell lines were grown in RPMI 1640 (Invitrogen, Life Technologies, Karlsruhe, Germany) supplemented with 10% FCS, 100 U/ml penicillin and 100 mg/ml streptomycin.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted using RNeasy Kit (Qiagen, Hilden, Germany) according to the manufacturer’s protocol.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Human Genome 133 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner3000.
| | Sample_data_processing | a CustomCDF file was used to annoatate the microarrays ( http://brainarray.mbni.med.umich.edu/Brainarray/Database/CustomCDF/genomic_curated_CDF.asp, version 10). The data were quantile normalized and analyzed with JMP Genomics based on mixed model ANOVA.
| | Sample_platform_id | GPL10925
| | Sample_contact_name | Li,,Li
| | Sample_contact_email | li.li@gmx.de
| | Sample_contact_institute | University Heidelberg
| | Sample_contact_address | Theodor-Kutzer-Ufer
| | Sample_contact_city | Mannheim
| | Sample_contact_zip/postal_code | 68167
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM594nnn/GSM594454/suppl/GSM594454.CEL.gz
| | Sample_series_id | GSE24155
| | Sample_data_row_count | 22244
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