Search results for the GEO ID: GSE24170  |
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GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM594721 | GPL570 |
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Set8_deltaPIP2
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U2OS
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cell line: U2OS
transfection: Set8_deltaPIP2
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U2OS cells were cultured in DMEM media with 10 % FBS and split daily at a 1:3 ratio.
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| Sample_geo_accession | GSM594721
| | Sample_status | Public on Sep 18 2010
| | Sample_submission_date | Sep 16 2010
| | Sample_last_update_date | Sep 17 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | U2OS cells were transduced with retroviruses encoding stable Set8b (Set8b_deltaPIP2), wt-Set8b or with empty vector (pMSCV-vector) and harvested 5 days post-trasduction according to standard procedures.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA extraction is according to the manufacturer's instructions (Invitrogen) using Trizole reagent.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Labeling and hybridyzation was performed accoding to the affymetrix protocol.
| | Sample_hyb_protocol | For studying gene expression, RNA was extracted from U2OS 5 days posttransduction with Set8b_deltaPIP2, wt-Set8 or pMSCV-vector control retroviruses, by using Trizol Invitrogen) and hybridized to the Human HG-U133_Plus_2 array as described in the Affymetrix GeneChIP protocol (Affymetrix).
| | Sample_scan_protocol | Each microarray was scanned, visualized, and analyzed for the level of each individual transcript using a GeneChIP Scanner 3000 and GeneChIP Operating Software (GCOS; Affymetrix). Transcript signal was mapped against the chromosome coordinates (probe-by-probe basis) using the HG-U133_Plus_2 Annotations, CSV provided by the manufacturer (Affymetrix)
| | Sample_data_processing | Each microarray was scanned and analyzed for signal intensities by GeneChIP Scanner 3000 and GeneChIP operating software software (Affymetrix).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Anindya,,Dutta
| | Sample_contact_email | ad8q@virginia.edu
| | Sample_contact_laboratory | Dutta Lab
| | Sample_contact_department | Biochemistry & Molecular Genetics
| | Sample_contact_institute | University Of Virginia
| | Sample_contact_address | 1300 Jefferson Park Ave
| | Sample_contact_city | Charlottesville
| | Sample_contact_state | VA
| | Sample_contact_zip/postal_code | 22908
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM594nnn/GSM594721/suppl/GSM594721_2009-072_bdp2_HG-U133_Plus_2_.CEL.gz
| | Sample_series_id | GSE24170
| | Sample_data_row_count | 54675
| | |
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GSM594722 | GPL570 |
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Wt-Set8b
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U2OS
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cell line: U2OS
transfection: Wt-Set8b
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U2OS cells were cultured in DMEM media with 10 % FBS and split daily at a 1:3 ratio.
|
| Sample_geo_accession | GSM594722
| | Sample_status | Public on Sep 18 2010
| | Sample_submission_date | Sep 16 2010
| | Sample_last_update_date | Sep 17 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | U2OS cells were transduced with retroviruses encoding stable Set8b (Set8b_deltaPIP2), wt-Set8b or with empty vector (pMSCV-vector) and harvested 5 days post-trasduction according to standard procedures.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA extraction is according to the manufacturer's instructions (Invitrogen) using Trizole reagent.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Labeling and hybridyzation was performed accoding to the affymetrix protocol.
| | Sample_hyb_protocol | For studying gene expression, RNA was extracted from U2OS 5 days posttransduction with Set8b_deltaPIP2, wt-Set8 or pMSCV-vector control retroviruses, by using Trizol Invitrogen) and hybridized to the Human HG-U133_Plus_2 array as described in the Affymetrix GeneChIP protocol (Affymetrix).
| | Sample_scan_protocol | Each microarray was scanned, visualized, and analyzed for the level of each individual transcript using a GeneChIP Scanner 3000 and GeneChIP Operating Software (GCOS; Affymetrix). Transcript signal was mapped against the chromosome coordinates (probe-by-probe basis) using the HG-U133_Plus_2 Annotations, CSV provided by the manufacturer (Affymetrix)
| | Sample_data_processing | Each microarray was scanned and analyzed for signal intensities by GeneChIP Scanner 3000 and GeneChIP operating software software (Affymetrix).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Anindya,,Dutta
| | Sample_contact_email | ad8q@virginia.edu
| | Sample_contact_laboratory | Dutta Lab
| | Sample_contact_department | Biochemistry & Molecular Genetics
| | Sample_contact_institute | University Of Virginia
| | Sample_contact_address | 1300 Jefferson Park Ave
| | Sample_contact_city | Charlottesville
| | Sample_contact_state | VA
| | Sample_contact_zip/postal_code | 22908
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM594nnn/GSM594722/suppl/GSM594722_2009-072_bWT_HG-U133_Plus_2_.CEL.gz
| | Sample_series_id | GSE24170
| | Sample_data_row_count | 54675
| | |
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GSM594723 | GPL570 |
|
pMSCV-vector
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U2OS
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cell line: U2OS
transfection: pMSCV-vector
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U2OS cells were cultured in DMEM media with 10 % FBS and split daily at a 1:3 ratio.
|
| Sample_geo_accession | GSM594723
| | Sample_status | Public on Sep 18 2010
| | Sample_submission_date | Sep 16 2010
| | Sample_last_update_date | Sep 17 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | U2OS cells were transduced with retroviruses encoding stable Set8b (Set8b_deltaPIP2), wt-Set8b or with empty vector (pMSCV-vector) and harvested 5 days post-trasduction according to standard procedures.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA extraction is according to the manufacturer's instructions (Invitrogen) using Trizole reagent.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Labeling and hybridyzation was performed accoding to the affymetrix protocol.
| | Sample_hyb_protocol | For studying gene expression, RNA was extracted from U2OS 5 days posttransduction with Set8b_deltaPIP2, wt-Set8 or pMSCV-vector control retroviruses, by using Trizol Invitrogen) and hybridized to the Human HG-U133_Plus_2 array as described in the Affymetrix GeneChIP protocol (Affymetrix).
| | Sample_scan_protocol | Each microarray was scanned, visualized, and analyzed for the level of each individual transcript using a GeneChIP Scanner 3000 and GeneChIP Operating Software (GCOS; Affymetrix). Transcript signal was mapped against the chromosome coordinates (probe-by-probe basis) using the HG-U133_Plus_2 Annotations, CSV provided by the manufacturer (Affymetrix)
| | Sample_data_processing | Each microarray was scanned and analyzed for signal intensities by GeneChIP Scanner 3000 and GeneChIP operating software software (Affymetrix).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Anindya,,Dutta
| | Sample_contact_email | ad8q@virginia.edu
| | Sample_contact_laboratory | Dutta Lab
| | Sample_contact_department | Biochemistry & Molecular Genetics
| | Sample_contact_institute | University Of Virginia
| | Sample_contact_address | 1300 Jefferson Park Ave
| | Sample_contact_city | Charlottesville
| | Sample_contact_state | VA
| | Sample_contact_zip/postal_code | 22908
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM594nnn/GSM594723/suppl/GSM594723_2009-072_vector_HG-U133_Plus_2_.CEL.gz
| | Sample_series_id | GSE24170
| | Sample_data_row_count | 54675
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