Search results for the GEO ID: GSE24190  |
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GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM595109 | GPL1261 |
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WT mouse keratinocytes
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Primary keratinocyte from neonatal WT mouse
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strain: ICR
tissue: skin
cell type: primary keratinocyte
genotype/variation: wild type
developmental stage: neonatal
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WT
S1101-035-WT 113005.CEL
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| Sample_geo_accession | GSM595109
| | Sample_status | Public on Sep 17 2010
| | Sample_submission_date | Sep 17 2010
| | Sample_last_update_date | Sep 12 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_growth_protocol_ch1 | Epidermal keratinocytes from skin tissue of neonatal mice were isolated and cultured in defined keratinocyte-Serum Free Medium (K-SFM) with growth supplement (Invitrogen) for 24 hr at 37C and 5% CO2 prior to extraction.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted by TRIzol in accordance with manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Expression 430 2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Chiou-Mei,,Lee
| | Sample_contact_email | lcm5132@mail.cgu.edu.tw
| | Sample_contact_department | Department of Medical Research and Development
| | Sample_contact_institute | Chang Gung Memorial Hospital
| | Sample_contact_address | 5, Fusing St
| | Sample_contact_city | Kwei-Shan
| | Sample_contact_state | Taoyuan
| | Sample_contact_zip/postal_code | 333
| | Sample_contact_country | Taiwan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM595nnn/GSM595109/suppl/GSM595109.CEL.gz
| | Sample_series_id | GSE24190
| | Sample_data_row_count | 45101
| | |
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GSM595110 | GPL1261 |
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RASSF9-/- mouse keratinocytes (sample 1 of 2)
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Primary keratinocyte from neonatal RASSF9-/- mouse
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strain: ICR
tissue: skin
cell type: primary keratinocyte
genotype/variation: RASSF9-/-
developmental stage: neonatal
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RASSF9-/-_1_of_2
S1101-036-KO 113005.CEL
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| Sample_geo_accession | GSM595110
| | Sample_status | Public on Sep 17 2010
| | Sample_submission_date | Sep 17 2010
| | Sample_last_update_date | Sep 12 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_growth_protocol_ch1 | Epidermal keratinocytes from skin tissue of neonatal mice were isolated and cultured in defined keratinocyte-Serum Free Medium (K-SFM) with growth supplement (Invitrogen) for 48 hr at 37C and 5% CO2 prior to extraction.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted by TRIzol in accordance with manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Expression 430 2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Chiou-Mei,,Lee
| | Sample_contact_email | lcm5132@mail.cgu.edu.tw
| | Sample_contact_department | Department of Medical Research and Development
| | Sample_contact_institute | Chang Gung Memorial Hospital
| | Sample_contact_address | 5, Fusing St
| | Sample_contact_city | Kwei-Shan
| | Sample_contact_state | Taoyuan
| | Sample_contact_zip/postal_code | 333
| | Sample_contact_country | Taiwan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM595nnn/GSM595110/suppl/GSM595110.CEL.gz
| | Sample_series_id | GSE24190
| | Sample_data_row_count | 45101
| | |
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GSM595111 | GPL1261 |
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RASSF9-/- mouse keratinocytes (sample 2 of 2)
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Primary keratinocyte from neonatal RASSF9-/- mouse
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strain: ICR
tissue: skin
cell type: primary keratinocyte
genotype/variation: RASSF9-/-
developmental stage: neonatal
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RASSF9-/-_2_of_2
S1101-037-KO 121305.CEL
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| Sample_geo_accession | GSM595111
| | Sample_status | Public on Sep 17 2010
| | Sample_submission_date | Sep 17 2010
| | Sample_last_update_date | Sep 12 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_growth_protocol_ch1 | Epidermal keratinocytes from skin tissue of neonatal mice were isolated and cultured in defined keratinocyte-Serum Free Medium (K-SFM) with growth supplement (Invitrogen) for 48 hr at 37C and 5% CO2 prior to extraction.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted by TRIzol in accordance with manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Expression 430 2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Chiou-Mei,,Lee
| | Sample_contact_email | lcm5132@mail.cgu.edu.tw
| | Sample_contact_department | Department of Medical Research and Development
| | Sample_contact_institute | Chang Gung Memorial Hospital
| | Sample_contact_address | 5, Fusing St
| | Sample_contact_city | Kwei-Shan
| | Sample_contact_state | Taoyuan
| | Sample_contact_zip/postal_code | 333
| | Sample_contact_country | Taiwan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM595nnn/GSM595111/suppl/GSM595111.CEL.gz
| | Sample_series_id | GSE24190
| | Sample_data_row_count | 45101
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