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Search results for the GEO ID: GSE2421
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GPL ID
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Title
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Description
Characteristics
GSM45580
GPL339
0-KO BMDM, not treated, KO Vdr -/- Vdr tm1 Rge knockout mice; Bone marrow derived macrophages (BMDMs); Briefly, femora and tibia of the hind legs were flushed with cold DMEM (Invitrogen) containing 10 % heat-inactivated fetal calf serum (Biowest), 200 mM L-glutamine, and penicillin/streptomycin (Invitrogen). This complete medium was supplemented with M-CSF (50 ng/ml) and bone marrow cells were cultured at 37°C in a humidified 5% CO2 atmosphere for 7 days. Thioglycolate-elicited macrophages were obtained 4 days after the intraperitoneal injection of sterile 4 % thioglycolate medium. Cells were harvested by peritoneal lavage with cold PBS, 1% FCS and were cultured in RPMI 1640 (Invitrogen) including 10 % heat-inactivated fetal calf serum, L-glutamine, and penicillin/streptomycin (P/S).
GSM45581
GPL339
0-WT wild type; BMDM; not treated wild type mice; Bone marrow derived macrophages (BMDMs); Briefly, femora and tibia of the hind legs were flushed with cold DMEM (Invitrogen) containing 10 % heat-inactivated fetal calf serum (Biowest), 200 mM L-glutamine, and penicillin/streptomycin (Invitrogen). This complete medium was supplemented with M-CSF (50 ng/ml) and bone marrow cells were cultured at 37°C in a humidified 5% CO2 atmosphere for 7 days. Thioglycolate-elicited macrophages were obtained 4 days after the intraperitoneal injection of sterile 4 % thioglycolate medium. Cells were harvested by peritoneal lavage with cold PBS, 1% FCS and were cultured in RPMI 1640 (Invitrogen) including 10 % heat-inactivated fetal calf serum, L-glutamine, and penicillin/streptomycin (P/S).
GSM45582
GPL339
Vit-WT wild type; BMDM; 1alpha,25(OH)2D3 treated wild type mice; Bone marrow derived macrophages (BMDMs); Briefly, femora and tibia of the hind legs were flushed with cold DMEM (Invitrogen) containing 10 % heat-inactivated fetal calf serum (Biowest), 200 mM L-glutamine, and penicillin/streptomycin (Invitrogen). This complete medium was supplemented with M-CSF (50 ng/ml) and bone marrow cells were cultured at 37°C in a humidified 5% CO2 atmosphere for 7 days. Thioglycolate-elicited macrophages were obtained 4 days after the intraperitoneal injection of sterile 4 % thioglycolate medium. Cells were harvested by peritoneal lavage with cold PBS, 1% FCS and were cultured in RPMI 1640 (Invitrogen) including 10 % heat-inactivated fetal calf serum, L-glutamine, and penicillin/streptomycin (P/S). Macrophages were plated at 8 x 10^4/well in 96-well plates and cultured in P/S-free medium in the presence or absence of IFN-gamma, 1alpha,25(OH)2D3, or both for 24-48 hours
GSM45583
GPL339
Vit-KO BMDM, 1alpha,25(OH)2D3 treated, KO Vdr -/- KO Vdr -/- mice; Bone marrow derived macrophages (BMDMs); Briefly, femora and tibia of the hind legs were flushed with cold DMEM (Invitrogen) containing 10 % heat-inactivated fetal calf serum (Biowest), 200 mM L-glutamine, and penicillin/streptomycin (Invitrogen). This complete medium was supplemented with M-CSF (50 ng/ml) and bone marrow cells were cultured at 37°C in a humidified 5% CO2 atmosphere for 7 days. Thioglycolate-elicited macrophages were obtained 4 days after the intraperitoneal injection of sterile 4 % thioglycolate medium. Cells were harvested by peritoneal lavage with cold PBS, 1% FCS and were cultured in RPMI 1640 (Invitrogen) including 10 % heat-inactivated fetal calf serum, L-glutamine, and penicillin/streptomycin (P/S). Macrophages were plated at 8 x 104/well in 96-well plates and cultured in P/S-free medium in the presence or absence of IFN-gamma, 1alpha,25(OH)2D3, or both for 24-48 hours.
GSM45584
GPL339
IFN-WT wild type; BMDM; IFNgamma treated wild type mice; Bone marrow derived macrophages (BMDMs); Briefly, femora and tibia of the hind legs were flushed with cold DMEM (Invitrogen) containing 10 % heat-inactivated fetal calf serum (Biowest), 200 mM L-glutamine, and penicillin/streptomycin (Invitrogen). This complete medium was supplemented with M-CSF (50 ng/ml) and bone marrow cells were cultured at 37°C in a humidified 5% CO2 atmosphere for 7 days. Thioglycolate-elicited macrophages were obtained 4 days after the intraperitoneal injection of sterile 4 % thioglycolate medium. Cells were harvested by peritoneal lavage with cold PBS, 1% FCS and were cultured in RPMI 1640 (Invitrogen) including 10 % heat-inactivated fetal calf serum, L-glutamine, and penicillin/streptomycin (P/S). Macrophages were plated at 8 x 104/well in 96-well plates and cultured in P/S-free medium in the presence or absence of IFN-gamma, 1alpha,25(OH)2D3, or both for 24-48 hours.
GSM45585
GPL339
IFN-KO BMDM, IFNgamma treated, KO Vdr -/- KO Vdr -/- mice; Bone marrow derived macrophages (BMDMs); Briefly, femora and tibia of the hind legs were flushed with cold DMEM (Invitrogen) containing 10 % heat-inactivated fetal calf serum (Biowest), 200 mM L-glutamine, and penicillin/streptomycin (Invitrogen). This complete medium was supplemented with M-CSF (50 ng/ml) and bone marrow cells were cultured at 37°C in a humidified 5% CO2 atmosphere for 7 days. Thioglycolate-elicited macrophages were obtained 4 days after the intraperitoneal injection of sterile 4 % thioglycolate medium. Cells were harvested by peritoneal lavage with cold PBS, 1% FCS and were cultured in RPMI 1640 (Invitrogen) including 10 % heat-inactivated fetal calf serum, L-glutamine, and penicillin/streptomycin (P/S). Macrophages were plated at 8 x 104/well in 96-well plates and cultured in P/S-free medium in the presence or absence of IFN-gamma, 1alpha,25(OH)2D3, or both for 24-48 hours.
GSM45586
GPL339
IFN/Vit-WT wild type; BMDM; IFNgamma and 1alpha,25(OH)2D3 treated wild type mice; Bone marrow derived macrophages (BMDMs); Briefly, femora and tibia of the hind legs were flushed with cold DMEM (Invitrogen) containing 10 % heat-inactivated fetal calf serum (Biowest), 200 mM L-glutamine, and penicillin/streptomycin (Invitrogen). This complete medium was supplemented with M-CSF (50 ng/ml) and bone marrow cells were cultured at 37°C in a humidified 5% CO2 atmosphere for 7 days. Thioglycolate-elicited macrophages were obtained 4 days after the intraperitoneal injection of sterile 4 % thioglycolate medium. Cells were harvested by peritoneal lavage with cold PBS, 1% FCS and were cultured in RPMI 1640 (Invitrogen) including 10 % heat-inactivated fetal calf serum, L-glutamine, and penicillin/streptomycin (P/S). Macrophages were plated at 8 x 104/well in 96-well plates and cultured in P/S-free medium in the presence or absence of IFN-gamma, 1alpha,25(OH)2D3, or both for 24-48 hours.
GSM45587
GPL339
IFN/Vit-KO BMDM, 1alpha,25(OH)2D3 and IFNgamma treated, KO Vdr -/- KO Vdr -/- mice; Bone marrow derived macrophages (BMDMs); Briefly, femora and tibia of the hind legs were flushed with cold DMEM (Invitrogen) containing 10 % heat-inactivated fetal calf serum (Biowest), 200 mM L-glutamine, and penicillin/streptomycin (Invitrogen). This complete medium was supplemented with M-CSF (50 ng/ml) and bone marrow cells were cultured at 37°C in a humidified 5% CO2 atmosphere for 7 days. Thioglycolate-elicited macrophages were obtained 4 days after the intraperitoneal injection of sterile 4 % thioglycolate medium. Cells were harvested by peritoneal lavage with cold PBS, 1% FCS and were cultured in RPMI 1640 (Invitrogen) including 10 % heat-inactivated fetal calf serum, L-glutamine, and penicillin/streptomycin (P/S). Macrophages were plated at 8 x 104/well in 96-well plates and cultured in P/S-free medium in the presence or absence of IFN-gamma, 1alpha,25(OH)2D3, or both for 24-48 hours.
 
 
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