Search results for the GEO ID: GSE24249  |
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(Once the selection is made, click on "Add groups" in "Make groups for comparison", to make a group. Scroll down) |
|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM596523 | GPL570 |
|
231_Empty_rep1
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breast cancer cell line transduced with eGFP
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cell line: breast cancer cell line MDA-MB-231
ectopic expression: control GFP
rep: 1
|
Raw data file: Chu_Empty_1_121508.CEL
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| Sample_geo_accession | GSM596523
| | Sample_status | Public on Sep 01 2011
| | Sample_submission_date | Sep 21 2010
| | Sample_last_update_date | Sep 01 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | MDA-MB-231 were transduced with a lentivirus expressing eGFP alone or GATA3 and eGFP and cells were sorted for GFP expression
| | Sample_growth_protocol_ch1 | MDA-MB-231 expressing control GFP or GATA3 were grown in DMEM/high glucose supplemented with 10% FBS, sodium pyruvate and pen/strep. 1.5 million cells were seeded into 100 mm dishes and RNA collected after 2 days.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was isolated by Trizol® according to the manufacturer's instructions. All samples used for microarray analysis had a high quality score (RIN >9).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | RNA (1 ug) was reverse transcribed with a T7-oligo(dT) primer and biotin labeled using the Affymetrix One Cycle Target Labeling kit (Affymetrix) following the manufacturer’s protocol.
| | Sample_hyb_protocol | Hybridized to Affymetrix human U133 plus 2.0 GeneChips
| | Sample_scan_protocol | Scanned on an Affymetrix GeneChip scanner 3000 and data collected using Affymetrix GCOS software
| | Sample_data_processing | Data was processed into log base 2 gene expression measures with gcRMA algorithm and quantile normalization. The data were processed in BRB-ArrayTools developed by Richard Simon and BRB-ArrayTools Development Team (http://linus.nci.nih.gov/BRB-ArrayTools.html).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jeffrey,E,Green
| | Sample_contact_email | JEGreen@mail.nih.gov
| | Sample_contact_phone | 3014355193
| | Sample_contact_fax | 3014968709
| | Sample_contact_institute | NCI
| | Sample_contact_address | Rm 4054, 37 Convent Drive
| | Sample_contact_city | Bethesda
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20892
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM596nnn/GSM596523/suppl/GSM596523.CEL.gz
| | Sample_series_id | GSE24249
| | Sample_data_row_count | 54675
| | |
|
GSM596524 | GPL570 |
|
231_Empty_rep2
|
breast cancer cell line transduced with eGFP
|
cell line: breast cancer cell line MDA-MB-231
ectopic expression: control GFP
rep: 2
|
Raw data file: Chu_Empty_2_121508.CEL
|
| Sample_geo_accession | GSM596524
| | Sample_status | Public on Sep 01 2011
| | Sample_submission_date | Sep 21 2010
| | Sample_last_update_date | Sep 01 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | MDA-MB-231 were transduced with a lentivirus expressing eGFP alone or GATA3 and eGFP and cells were sorted for GFP expression
| | Sample_growth_protocol_ch1 | MDA-MB-231 expressing control GFP or GATA3 were grown in DMEM/high glucose supplemented with 10% FBS, sodium pyruvate and pen/strep. 1.5 million cells were seeded into 100 mm dishes and RNA collected after 2 days.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was isolated by Trizol® according to the manufacturer's instructions. All samples used for microarray analysis had a high quality score (RIN >9).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | RNA (1 ug) was reverse transcribed with a T7-oligo(dT) primer and biotin labeled using the Affymetrix One Cycle Target Labeling kit (Affymetrix) following the manufacturer’s protocol.
| | Sample_hyb_protocol | Hybridized to Affymetrix human U133 plus 2.0 GeneChips
| | Sample_scan_protocol | Scanned on an Affymetrix GeneChip scanner 3000 and data collected using Affymetrix GCOS software
| | Sample_data_processing | Data was processed into log base 2 gene expression measures with gcRMA algorithm and quantile normalization. The data were processed in BRB-ArrayTools developed by Richard Simon and BRB-ArrayTools Development Team (http://linus.nci.nih.gov/BRB-ArrayTools.html).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jeffrey,E,Green
| | Sample_contact_email | JEGreen@mail.nih.gov
| | Sample_contact_phone | 3014355193
| | Sample_contact_fax | 3014968709
| | Sample_contact_institute | NCI
| | Sample_contact_address | Rm 4054, 37 Convent Drive
| | Sample_contact_city | Bethesda
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20892
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM596nnn/GSM596524/suppl/GSM596524.CEL.gz
| | Sample_series_id | GSE24249
| | Sample_data_row_count | 54675
| | |
|
GSM596525 | GPL570 |
|
231-Empty_rep3
|
breast cancer cell line transduced with eGFP
|
cell line: breast cancer cell line MDA-MB-231
ectopic expression: control GFP
rep: 3
|
Raw data file: Chu_Empty_3_121508.CEL
|
| Sample_geo_accession | GSM596525
| | Sample_status | Public on Sep 01 2011
| | Sample_submission_date | Sep 21 2010
| | Sample_last_update_date | Sep 01 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | MDA-MB-231 were transduced with a lentivirus expressing eGFP alone or GATA3 and eGFP and cells were sorted for GFP expression
| | Sample_growth_protocol_ch1 | MDA-MB-231 expressing control GFP or GATA3 were grown in DMEM/high glucose supplemented with 10% FBS, sodium pyruvate and pen/strep. 1.5 million cells were seeded into 100 mm dishes and RNA collected after 2 days.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was isolated by Trizol® according to the manufacturer's instructions. All samples used for microarray analysis had a high quality score (RIN >9).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | RNA (1 ug) was reverse transcribed with a T7-oligo(dT) primer and biotin labeled using the Affymetrix One Cycle Target Labeling kit (Affymetrix) following the manufacturer’s protocol.
| | Sample_hyb_protocol | Hybridized to Affymetrix human U133 plus 2.0 GeneChips
| | Sample_scan_protocol | Scanned on an Affymetrix GeneChip scanner 3000 and data collected using Affymetrix GCOS software
| | Sample_data_processing | Data was processed into log base 2 gene expression measures with gcRMA algorithm and quantile normalization. The data were processed in BRB-ArrayTools developed by Richard Simon and BRB-ArrayTools Development Team (http://linus.nci.nih.gov/BRB-ArrayTools.html).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jeffrey,E,Green
| | Sample_contact_email | JEGreen@mail.nih.gov
| | Sample_contact_phone | 3014355193
| | Sample_contact_fax | 3014968709
| | Sample_contact_institute | NCI
| | Sample_contact_address | Rm 4054, 37 Convent Drive
| | Sample_contact_city | Bethesda
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20892
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM596nnn/GSM596525/suppl/GSM596525.CEL.gz
| | Sample_series_id | GSE24249
| | Sample_data_row_count | 54675
| | |
|
GSM596526 | GPL570 |
|
231_GATA3_rep1
|
breast cancer cell line transduced with GATA3
|
cell line: breast cancer cell line MDA-MB-231
ectopic expression: GATA3 and GFP
rep: 1
|
Raw data file: Chu_GATA_1_121508.CEL
|
| Sample_geo_accession | GSM596526
| | Sample_status | Public on Sep 01 2011
| | Sample_submission_date | Sep 21 2010
| | Sample_last_update_date | Sep 01 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | MDA-MB-231 were transduced with a lentivirus expressing eGFP alone or GATA3 and eGFP and cells were sorted for GFP expression
| | Sample_growth_protocol_ch1 | MDA-MB-231 expressing control GFP or GATA3 were grown in DMEM/high glucose supplemented with 10% FBS, sodium pyruvate and pen/strep. 1.5 million cells were seeded into 100 mm dishes and RNA collected after 2 days.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was isolated by Trizol® according to the manufacturer's instructions. All samples used for microarray analysis had a high quality score (RIN >9).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | RNA (1 ug) was reverse transcribed with a T7-oligo(dT) primer and biotin labeled using the Affymetrix One Cycle Target Labeling kit (Affymetrix) following the manufacturer’s protocol.
| | Sample_hyb_protocol | Hybridized to Affymetrix human U133 plus 2.0 GeneChips
| | Sample_scan_protocol | Scanned on an Affymetrix GeneChip scanner 3000 and data collected using Affymetrix GCOS software
| | Sample_data_processing | Data was processed into log base 2 gene expression measures with gcRMA algorithm and quantile normalization. The data were processed in BRB-ArrayTools developed by Richard Simon and BRB-ArrayTools Development Team (http://linus.nci.nih.gov/BRB-ArrayTools.html).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jeffrey,E,Green
| | Sample_contact_email | JEGreen@mail.nih.gov
| | Sample_contact_phone | 3014355193
| | Sample_contact_fax | 3014968709
| | Sample_contact_institute | NCI
| | Sample_contact_address | Rm 4054, 37 Convent Drive
| | Sample_contact_city | Bethesda
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20892
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM596nnn/GSM596526/suppl/GSM596526.CEL.gz
| | Sample_series_id | GSE24249
| | Sample_data_row_count | 54675
| | |
|
GSM596527 | GPL570 |
|
231_GATA3_rep2
|
breast cancer cell line transduced with GATA3
|
cell line: breast cancer cell line MDA-MB-231
ectopic expression: GATA3 and GFP
rep: 2
|
Raw data file: Chu_GATA_2_121508.CEL
|
| Sample_geo_accession | GSM596527
| | Sample_status | Public on Sep 01 2011
| | Sample_submission_date | Sep 21 2010
| | Sample_last_update_date | Sep 01 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | MDA-MB-231 were transduced with a lentivirus expressing eGFP alone or GATA3 and eGFP and cells were sorted for GFP expression
| | Sample_growth_protocol_ch1 | MDA-MB-231 expressing control GFP or GATA3 were grown in DMEM/high glucose supplemented with 10% FBS, sodium pyruvate and pen/strep. 1.5 million cells were seeded into 100 mm dishes and RNA collected after 2 days.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was isolated by Trizol® according to the manufacturer's instructions. All samples used for microarray analysis had a high quality score (RIN >9).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | RNA (1 ug) was reverse transcribed with a T7-oligo(dT) primer and biotin labeled using the Affymetrix One Cycle Target Labeling kit (Affymetrix) following the manufacturer’s protocol.
| | Sample_hyb_protocol | Hybridized to Affymetrix human U133 plus 2.0 GeneChips
| | Sample_scan_protocol | Scanned on an Affymetrix GeneChip scanner 3000 and data collected using Affymetrix GCOS software
| | Sample_data_processing | Data was processed into log base 2 gene expression measures with gcRMA algorithm and quantile normalization. The data were processed in BRB-ArrayTools developed by Richard Simon and BRB-ArrayTools Development Team (http://linus.nci.nih.gov/BRB-ArrayTools.html).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jeffrey,E,Green
| | Sample_contact_email | JEGreen@mail.nih.gov
| | Sample_contact_phone | 3014355193
| | Sample_contact_fax | 3014968709
| | Sample_contact_institute | NCI
| | Sample_contact_address | Rm 4054, 37 Convent Drive
| | Sample_contact_city | Bethesda
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20892
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM596nnn/GSM596527/suppl/GSM596527.CEL.gz
| | Sample_series_id | GSE24249
| | Sample_data_row_count | 54675
| | |
|
GSM596528 | GPL570 |
|
231_GATA3_rep3
|
breast cancer cell line transduced with GATA3
|
cell line: breast cancer cell line MDA-MB-231
ectopic expression: GATA3 and GFP
rep: 3
|
Raw data file: Chu_GATA_3_122308.CEL
|
| Sample_geo_accession | GSM596528
| | Sample_status | Public on Sep 01 2011
| | Sample_submission_date | Sep 21 2010
| | Sample_last_update_date | Sep 01 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | MDA-MB-231 were transduced with a lentivirus expressing eGFP alone or GATA3 and eGFP and cells were sorted for GFP expression
| | Sample_growth_protocol_ch1 | MDA-MB-231 expressing control GFP or GATA3 were grown in DMEM/high glucose supplemented with 10% FBS, sodium pyruvate and pen/strep. 1.5 million cells were seeded into 100 mm dishes and RNA collected after 2 days.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was isolated by Trizol® according to the manufacturer's instructions. All samples used for microarray analysis had a high quality score (RIN >9).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | RNA (1 ug) was reverse transcribed with a T7-oligo(dT) primer and biotin labeled using the Affymetrix One Cycle Target Labeling kit (Affymetrix) following the manufacturer’s protocol.
| | Sample_hyb_protocol | Hybridized to Affymetrix human U133 plus 2.0 GeneChips
| | Sample_scan_protocol | Scanned on an Affymetrix GeneChip scanner 3000 and data collected using Affymetrix GCOS software
| | Sample_data_processing | Data was processed into log base 2 gene expression measures with gcRMA algorithm and quantile normalization. The data were processed in BRB-ArrayTools developed by Richard Simon and BRB-ArrayTools Development Team (http://linus.nci.nih.gov/BRB-ArrayTools.html).
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jeffrey,E,Green
| | Sample_contact_email | JEGreen@mail.nih.gov
| | Sample_contact_phone | 3014355193
| | Sample_contact_fax | 3014968709
| | Sample_contact_institute | NCI
| | Sample_contact_address | Rm 4054, 37 Convent Drive
| | Sample_contact_city | Bethesda
| | Sample_contact_state | MD
| | Sample_contact_zip/postal_code | 20892
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM596nnn/GSM596528/suppl/GSM596528.CEL.gz
| | Sample_series_id | GSE24249
| | Sample_data_row_count | 54675
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