Search results for the GEO ID: GSE24291  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM597486 | GPL1261 |
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Day 2.25 of ES cell differentiation, untreated
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A2.snail, DKK treatment, day 2.25
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cell type: mouse embryonic stem cells
cell line: A2.snail
protocol: control
agent: untreated
time: day 2.25 day of differentiation
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ESCSnail_DKK_6h.CEL
Gene expression data from day 2.25 of differentiation of ES cells in DKK1
|
| Sample_geo_accession | GSM597486
| | Sample_status | Public on Sep 22 2011
| | Sample_submission_date | Sep 23 2010
| | Sample_last_update_date | Sep 22 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | In DKKdox conditions, doxycycline was added for a final concentration of 250ng/mL on day 2
| | Sample_growth_protocol_ch1 | ES cells were differentiated as embryoid bodies in petri dishes at a density of 15,000 cells/mL in differentiation media consisting of 10% FCS and DKK1.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was isolated using Qiagen RNeasy kit per the manufacture's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 ug total RNA
| | Sample_hyb_protocol | The Affymetrix operating system (Command Console) was used to manage the washing and hybridization steps.
| | Sample_scan_protocol | The Affymetrix operating system (Command Console) was used for scanning. Affymetrix analysis software (Genotyping Console) was used to derive Quality Control metrics, create .cel files and scaled expression data from the scanned Expresison array image files
| | Sample_data_processing | The data were analyzed with dChip 2008 using default analysis settings. All samples were normalized to a single baseline array of median intensity (87).
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Jennifer,,Gill
| | Sample_contact_laboratory | Kenneth Murphy
| | Sample_contact_department | Pathology/Immunology
| | Sample_contact_institute | Washington University School of Medicine
| | Sample_contact_address | 660 South Euclid Ave.
| | Sample_contact_city | Saint Louis
| | Sample_contact_state | MO
| | Sample_contact_zip/postal_code | 63110
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM597nnn/GSM597486/suppl/GSM597486.CEL.gz
| | Sample_series_id | GSE24291
| | Sample_data_row_count | 45037
| | |
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GSM597487 | GPL1261 |
|
Day 2.25 of ES cell differentiation, dox-induced Snail
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A2.snail, DKK+dox treatment, day 2.25
|
cell type: mouse embryonic stem cells
cell line: A2.snail
protocol: inducible expression of Snail
agent: doxycycline
dose: 250 ng/mL
time post-dox: 6 hrs
time: day 2.25 day of differentiation
|
ESCSnail_DKKdox_6h.CEL
Gene expression data from day 2.25 of differentiation of ES cells inducibly expressing Snail in DKK1
|
| Sample_geo_accession | GSM597487
| | Sample_status | Public on Sep 22 2011
| | Sample_submission_date | Sep 23 2010
| | Sample_last_update_date | Sep 22 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | In DKKdox conditions, doxycycline was added for a final concentration of 250ng/mL on day 2
| | Sample_growth_protocol_ch1 | ES cells were differentiated as embryoid bodies in petri dishes at a density of 15,000 cells/mL in differentiation media consisting of 10% FCS and DKK1.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was isolated using Qiagen RNeasy kit per the manufacture's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 ug total RNA
| | Sample_hyb_protocol | The Affymetrix operating system (Command Console) was used to manage the washing and hybridization steps.
| | Sample_scan_protocol | The Affymetrix operating system (Command Console) was used for scanning. Affymetrix analysis software (Genotyping Console) was used to derive Quality Control metrics, create .cel files and scaled expression data from the scanned Expresison array image files
| | Sample_data_processing | The data were analyzed with dChip 2008 using default analysis settings. All samples were normalized to a single baseline array of median intensity (87).
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Jennifer,,Gill
| | Sample_contact_laboratory | Kenneth Murphy
| | Sample_contact_department | Pathology/Immunology
| | Sample_contact_institute | Washington University School of Medicine
| | Sample_contact_address | 660 South Euclid Ave.
| | Sample_contact_city | Saint Louis
| | Sample_contact_state | MO
| | Sample_contact_zip/postal_code | 63110
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM597nnn/GSM597487/suppl/GSM597487.CEL.gz
| | Sample_series_id | GSE24291
| | Sample_data_row_count | 45037
| | |
|
GSM597488 | GPL1261 |
|
Day 2.5 of ES cell differentiation, untreated
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A2.snail, DKK treatment, day 2.5
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cell type: mouse embryonic stem cells
cell line: A2.snail
protocol: control
agent: untreated
time: day 2.5 day of differentiation
|
ESCSnail_DKK_12h.CEL
Gene expression data from day 2.5 of differentiation of ES cells in DKK1
|
| Sample_geo_accession | GSM597488
| | Sample_status | Public on Sep 22 2011
| | Sample_submission_date | Sep 23 2010
| | Sample_last_update_date | Sep 22 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | In DKKdox conditions, doxycycline was added for a final concentration of 250ng/mL on day 2
| | Sample_growth_protocol_ch1 | ES cells were differentiated as embryoid bodies in petri dishes at a density of 15,000 cells/mL in differentiation media consisting of 10% FCS and DKK1.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was isolated using Qiagen RNeasy kit per the manufacture's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 ug total RNA
| | Sample_hyb_protocol | The Affymetrix operating system (Command Console) was used to manage the washing and hybridization steps.
| | Sample_scan_protocol | The Affymetrix operating system (Command Console) was used for scanning. Affymetrix analysis software (Genotyping Console) was used to derive Quality Control metrics, create .cel files and scaled expression data from the scanned Expresison array image files
| | Sample_data_processing | The data were analyzed with dChip 2008 using default analysis settings. All samples were normalized to a single baseline array of median intensity (87).
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Jennifer,,Gill
| | Sample_contact_laboratory | Kenneth Murphy
| | Sample_contact_department | Pathology/Immunology
| | Sample_contact_institute | Washington University School of Medicine
| | Sample_contact_address | 660 South Euclid Ave.
| | Sample_contact_city | Saint Louis
| | Sample_contact_state | MO
| | Sample_contact_zip/postal_code | 63110
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM597nnn/GSM597488/suppl/GSM597488.CEL.gz
| | Sample_series_id | GSE24291
| | Sample_data_row_count | 45037
| | |
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GSM597489 | GPL1261 |
|
Day 2.5 of ES cell differentiation, dox-induced Snail
|
A2.snail, DKK+dox treatment, day 2.5
|
cell type: mouse embryonic stem cells
cell line: A2.snail
protocol: inducible expression of Snail
agent: doxycycline
dose: 250 ng/mL
time post-dox: 12 hrs
time: day 2.5 day of differentiation
|
ESCSnail_DKKdox_12h.CEL
Gene expression data from day 2.5 of differentiation of ES cells inducibly expressing Snail in DKK1
|
| Sample_geo_accession | GSM597489
| | Sample_status | Public on Sep 22 2011
| | Sample_submission_date | Sep 23 2010
| | Sample_last_update_date | Sep 22 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | In DKKdox conditions, doxycycline was added for a final concentration of 250ng/mL on day 2
| | Sample_growth_protocol_ch1 | ES cells were differentiated as embryoid bodies in petri dishes at a density of 15,000 cells/mL in differentiation media consisting of 10% FCS and DKK1.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was isolated using Qiagen RNeasy kit per the manufacture's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 ug total RNA
| | Sample_hyb_protocol | The Affymetrix operating system (Command Console) was used to manage the washing and hybridization steps.
| | Sample_scan_protocol | The Affymetrix operating system (Command Console) was used for scanning. Affymetrix analysis software (Genotyping Console) was used to derive Quality Control metrics, create .cel files and scaled expression data from the scanned Expresison array image files
| | Sample_data_processing | The data were analyzed with dChip 2008 using default analysis settings. All samples were normalized to a single baseline array of median intensity (87).
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Jennifer,,Gill
| | Sample_contact_laboratory | Kenneth Murphy
| | Sample_contact_department | Pathology/Immunology
| | Sample_contact_institute | Washington University School of Medicine
| | Sample_contact_address | 660 South Euclid Ave.
| | Sample_contact_city | Saint Louis
| | Sample_contact_state | MO
| | Sample_contact_zip/postal_code | 63110
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM597nnn/GSM597489/suppl/GSM597489.CEL.gz
| | Sample_series_id | GSE24291
| | Sample_data_row_count | 45037
| | |
|
GSM597490 | GPL1261 |
|
Day 3 of ES cell differentiation, untreated
|
A2.snail, DKK treatment, day 3
|
cell type: mouse embryonic stem cells
cell line: A2.snail
protocol: control
agent: untreated
time: day 3 day of differentiation
|
ESCSnail_DKK_24h.CEL
Gene expression data from day 3 of differentiation of ES cells in DKK1
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| Sample_geo_accession | GSM597490
| | Sample_status | Public on Sep 22 2011
| | Sample_submission_date | Sep 23 2010
| | Sample_last_update_date | Sep 22 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | In DKKdox conditions, doxycycline was added for a final concentration of 250ng/mL on day 2
| | Sample_growth_protocol_ch1 | ES cells were differentiated as embryoid bodies in petri dishes at a density of 15,000 cells/mL in differentiation media consisting of 10% FCS and DKK1.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was isolated using Qiagen RNeasy kit per the manufacture's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 ug total RNA
| | Sample_hyb_protocol | The Affymetrix operating system (Command Console) was used to manage the washing and hybridization steps.
| | Sample_scan_protocol | The Affymetrix operating system (Command Console) was used for scanning. Affymetrix analysis software (Genotyping Console) was used to derive Quality Control metrics, create .cel files and scaled expression data from the scanned Expresison array image files
| | Sample_data_processing | The data were analyzed with dChip 2008 using default analysis settings. All samples were normalized to a single baseline array of median intensity (87).
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Jennifer,,Gill
| | Sample_contact_laboratory | Kenneth Murphy
| | Sample_contact_department | Pathology/Immunology
| | Sample_contact_institute | Washington University School of Medicine
| | Sample_contact_address | 660 South Euclid Ave.
| | Sample_contact_city | Saint Louis
| | Sample_contact_state | MO
| | Sample_contact_zip/postal_code | 63110
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM597nnn/GSM597490/suppl/GSM597490.CEL.gz
| | Sample_series_id | GSE24291
| | Sample_data_row_count | 45037
| | |
|
GSM597491 | GPL1261 |
|
Day 3 of ES cell differentiation, dox-induced Snail
|
A2.snail, DKK+dox treatment, day 3
|
cell type: mouse embryonic stem cells
cell line: A2.snail
protocol: inducible expression of Snail
agent: doxycycline
dose: 250 ng/mL
time post-dox: 24 hrs
time: day 3 day of differentiation
|
ESCSnail_DKKdox_24h.CEL
Gene expression data from day 3 of differentiation of ES cells inducibly expressing Snail in DKK1
|
| Sample_geo_accession | GSM597491
| | Sample_status | Public on Sep 22 2011
| | Sample_submission_date | Sep 23 2010
| | Sample_last_update_date | Sep 22 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | In DKKdox conditions, doxycycline was added for a final concentration of 250ng/mL on day 2
| | Sample_growth_protocol_ch1 | ES cells were differentiated as embryoid bodies in petri dishes at a density of 15,000 cells/mL in differentiation media consisting of 10% FCS and DKK1.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was isolated using Qiagen RNeasy kit per the manufacture's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 ug total RNA
| | Sample_hyb_protocol | The Affymetrix operating system (Command Console) was used to manage the washing and hybridization steps.
| | Sample_scan_protocol | The Affymetrix operating system (Command Console) was used for scanning. Affymetrix analysis software (Genotyping Console) was used to derive Quality Control metrics, create .cel files and scaled expression data from the scanned Expresison array image files
| | Sample_data_processing | The data were analyzed with dChip 2008 using default analysis settings. All samples were normalized to a single baseline array of median intensity (87).
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Jennifer,,Gill
| | Sample_contact_laboratory | Kenneth Murphy
| | Sample_contact_department | Pathology/Immunology
| | Sample_contact_institute | Washington University School of Medicine
| | Sample_contact_address | 660 South Euclid Ave.
| | Sample_contact_city | Saint Louis
| | Sample_contact_state | MO
| | Sample_contact_zip/postal_code | 63110
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM597nnn/GSM597491/suppl/GSM597491.CEL.gz
| | Sample_series_id | GSE24291
| | Sample_data_row_count | 45037
| | |
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