Home   Search   Browse   Statistics   User guide   FAQs   Post question   Download   Process data  
Search results for the GEO ID: GSE2435
(Click on the check boxes provided under "Select for analysis", to initiate grouping)
(Once the selection is made, click on "Add groups" in "Make groups for comparison", to make a group. Scroll down)
GSM ID
GPL ID
Select for analysis
Title
Source name
Description
Characteristics
GSM45796
GPL570
autoph_6h_1 Awells cell line, 6h autophagy Aim: Assessment of mRNA expression changes in a cell line after 6 h and 24 h induction of autophagy by starvation Sample: B-lymphoblastoid cell line Awells (IHW-No. 9090) Culture conditions: 6 h in Hank's Balanced Salt Solution (HBSS) (37°C) to induce autophagy by starvation RNA isolation: TRIzol (Invitrogen) followed by RNeasy-cleanup (QIAGEN) Quality control: 2100 Bioanalyzer (Agilent), high quality RNA cDNA synthesis and labeling: Standard Affymetrix protocol, BioArray High Yield RNA Transcript Labeling Kit (ENZO) Hybridization: Standard Affymetrix EukGE-WS2v4 protocol Scanning: Affymetrix GeneChip 3000 scanner Normalization: Scaling over all probe sets, target intensity = 100 Keywords = autophagy
GSM45797
GPL570
autoph_24h_1 Awells cell line, 24h autophagy, replicate 1 of 2 Aim: Assessment of mRNA expression changes in a cell line after 6 h and 24 h induction of autophagy by starvation Sample: B-lymphoblastoid cell line Awells (IHW-No. 9090) Culture conditions: 24 h in Hank's Balanced Salt Solution (HBSS) (37°C) to induce autophagy by starvation RNA isolation: TRIzol (Invitrogen) followed by RNeasy-cleanup (QIAGEN) Quality control: 2100 Bioanalyzer (Agilent), high quality RNA cDNA synthesis and labeling: Standard Affymetrix protocol, BioArray High Yield RNA Transcript Labeling Kit (ENZO) Hybridization: Standard Affymetrix EukGE-WS2v4 protocol Scanning: Affymetrix GeneChip 3000 scanner Normalization: Scaling over all probe sets, target intensity = 100 Keywords = autophagy
GSM45798
GPL570
autoph_24h_2 Awells cell line, 24h autophagy, replicate 2 of 2 Aim: Assessment of mRNA expression changes in a cell line after 6 h and 24 h induction of autophagy by starvation Sample: B-lymphoblastoid cell line Awells (IHW-No. 9090) Culture conditions: 24 h in Hank's Balanced Salt Solution (HBSS) (37°C) to induce autophagy by starvation RNA isolation: TRIzol (Invitrogen) followed by RNeasy-cleanup (QIAGEN) Quality control: 2100 Bioanalyzer (Agilent), high quality RNA cDNA synthesis and labeling: Standard Affymetrix protocol, BioArray High Yield RNA Transcript Labeling Kit (ENZO) Hybridization: Standard Affymetrix EukGE-WS2v4 protocol Scanning: Affymetrix GeneChip 3000 scanner Normalization: Scaling over all probe sets, target intensity = 100 Keywords = autophagy
GSM45799
GPL570
contr_6h_1 Awells cell line, 6h control Aim: Assessment of mRNA expression changes in a cell line after 6 h and 24 h induction of autophagy by starvation Sample: B-lymphoblastoid cell line Awells (IHW-No. 9090) Culture conditions: 6 h in DMEM, 10% FCS, 37°C, to obtain background expression under standard culture conditions RNA isolation: TRIzol (Invitrogen) followed by RNeasy-cleanup (QIAGEN) Quality control: 2100 Bioanalyzer (Agilent), high quality RNA cDNA synthesis and labeling: Standard Affymetrix protocol, BioArray High Yield RNA Transcript Labeling Kit (ENZO) Hybridization: Standard Affymetrix EukGE-WS2v4 protocol Scanning: Affymetrix GeneChip 3000 scanner Normalization: Scaling over all probe sets, target intensity = 100 Keywords = autophagy
GSM45800
GPL570
contr_24h_1 Awells cell line, 24h control, replicate 1 of 2 Aim: Assessment of mRNA expression changes in a cell line after 6 h and 24 h induction of autophagy by starvation Sample: B-lymphoblastoid cell line Awells (IHW-No. 9090) Culture conditions: 24 h in DMEM, 10% FCS, 37°C, to obtain background expression under standard culture conditions RNA isolation: TRIzol (Invitrogen) followed by RNeasy-cleanup (QIAGEN) Quality control: 2100 Bioanalyzer (Agilent), high quality RNA cDNA synthesis and labeling: Standard Affymetrix protocol, BioArray High Yield RNA Transcript Labeling Kit (ENZO) Hybridization: Standard Affymetrix EukGE-WS2v4 protocol Scanning: Affymetrix GeneChip 3000 scanner Normalization: Scaling over all probe sets, target intensity = 100 Keywords = autophagy
GSM45801
GPL570
contr_24h_2 Awells cell line, 24h control, replicate 2 of 2 Aim: Assessment of mRNA expression changes in a cell line after 6 h and 24 h induction of autophagy by starvation Sample: B-lymphoblastoid cell line Awells (IHW-No. 9090) Culture conditions: 24 h in DMEM, 10% FCS, 37°C, to obtain background expression under standard culture conditions RNA isolation: TRIzol (Invitrogen) followed by RNeasy-cleanup (QIAGEN) Quality control: 2100 Bioanalyzer (Agilent), high quality RNA cDNA synthesis and labeling: Standard Affymetrix protocol, BioArray High Yield RNA Transcript Labeling Kit (ENZO) Hybridization: Standard Affymetrix EukGE-WS2v4 protocol Scanning: Affymetrix GeneChip 3000 scanner Normalization: Scaling over all probe sets, target intensity = 100 Keywords = autophagy
 
 
Make groups for comparisons
(2 groups will be compared at a time)
Select GSMs and click on "Add groups"
Enter the group name here:


Select expression type
Transcripts profile based on;
A. Differential status (Up/Down regulation)
B. Absolute calls (Transcribed/Not-detected)
 
Filter results by number of probes