Search results for the GEO ID: GSE24422  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM601621 | GPL570 |
|
Mixed_rep 1
|
hMSC adipocytes
|
cell type: mixed adipocyte and stromal
|
|
| Sample_geo_accession | GSM601621
| | Sample_status | Public on Nov 30 2010
| | Sample_submission_date | Sep 28 2010
| | Sample_last_update_date | Sep 28 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | On Day 19 of differentiation, cells were treated with 0 or 10ng/ml TNF and incubated for 24hrs. Cells then underwent a 5 hr serum starvation followed by teatment with 0 or 20nM insulin for 2 hrs. At the end of the incubation, cells were harvested ( mixed) or fractioanted into adipocyte ( buiyant) and stromal (pelleted) fractions and subjected to RNA isolation.
| | Sample_growth_protocol_ch1 | Human mesenchymal stem cells were purchased from Lonza and cultured in basal media (Lonza PT3238) with mesenchymal stem cell growth supplement (Lonza PT4105). Cells were seeded at a density of 5000 cells/cm2. At confluence, the cells were differentiated into adipocytes using the adipogenic differentiation kit (Lonza PT3004). The total differentiation process took 19 days, with 14 days of induction and 5 days of maintenance. All media were from Lonza, except that the insulin concentration (in both induction and maintenance media) was adjusted to 120 pM. Mature adipocytes were subjected to 1% formaldehyde cross-linking for 10 minutes at room temperature and neutralized with 0.125M glycine for 5 minutes. Cells were then treated with Accutase and scraped. The preparation was centrifuged for 25 minutes at 15°C at 3500 rpm. The adipocytes, formed as a white layer (anti-pellet) at the meniscus, were harvested and snap-frozen in liquid nitrogen.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Frozen cells were resuspended in Qiagen RLT buffer and total RNA isolated using the Qiagen RNeasy Kit.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | cDNA was synthesized using 1-2 ug of high quality total RNA. Supercript II™ (Invitrogen) was used to reverse-transcribe the mRNA in the presence of a T7 containing oligo dT24 primer followed by second strand synthesis as recommended by Affymetrix. cDNA was purified using Agencourt RNAClean magnetic beads and used as a template for in vitro transcription using the GeneChip® One-Cycle Labeling Kit from Affymetrix. cRNA was purified using Agencourt magnetic beads and the quantity and purity of the cRNA was determined by absorbance at 260 nm and 260/280 absorbance ratio respectively. The quality of the cRNA was evaluated by assessing the size distribution of the cRNA using a 1.25% MOPS gel.
| | Sample_hyb_protocol | The labeled cRNA was fragmented as recommended by Affymetrix and 10 µg was added to a hybridization cocktail prior to loading onto individual HG-U133_Plus_2 GeneChip®. The microarrays were hybridized at 45°C for 16- 24 hours and washed and stained on an Affymetrix FS450 fluidics station according to manufacturer recommendations.
| | Sample_scan_protocol | The microarrays were scanned on a GeneChip® Scanner 3000.
| | Sample_data_processing | All of the array files were normalized using the Robust Multi-Array Average (RMA) method
| | Sample_platform_id | GPL570
| | Sample_contact_name | Shelley ,,des Etages
| | Sample_contact_email | shelley.g.desetages@pfizer.com
| | Sample_contact_institute | Pfizer Inc.
| | Sample_contact_address | Eastern Point Road
| | Sample_contact_city | Groton
| | Sample_contact_state | CT
| | Sample_contact_zip/postal_code | 06340
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM601nnn/GSM601621/suppl/GSM601621_Exp_131_3434_h2_mixed_no_TNF_no_insulin_1.CEL.gz
| | Sample_series_id | GSE24422
| | Sample_data_row_count | 54675
| | |
|
GSM601622 | GPL570 |
|
Mixed_rep 2
|
hMSC adipocytes
|
cell type: mixed adipocyte and stromal
|
|
| Sample_geo_accession | GSM601622
| | Sample_status | Public on Nov 30 2010
| | Sample_submission_date | Sep 28 2010
| | Sample_last_update_date | Sep 28 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | On Day 19 of differentiation, cells were treated with 0 or 10ng/ml TNF and incubated for 24hrs. Cells then underwent a 5 hr serum starvation followed by teatment with 0 or 20nM insulin for 2 hrs. At the end of the incubation, cells were harvested ( mixed) or fractioanted into adipocyte ( buiyant) and stromal (pelleted) fractions and subjected to RNA isolation.
| | Sample_growth_protocol_ch1 | Human mesenchymal stem cells were purchased from Lonza and cultured in basal media (Lonza PT3238) with mesenchymal stem cell growth supplement (Lonza PT4105). Cells were seeded at a density of 5000 cells/cm2. At confluence, the cells were differentiated into adipocytes using the adipogenic differentiation kit (Lonza PT3004). The total differentiation process took 19 days, with 14 days of induction and 5 days of maintenance. All media were from Lonza, except that the insulin concentration (in both induction and maintenance media) was adjusted to 120 pM. Mature adipocytes were subjected to 1% formaldehyde cross-linking for 10 minutes at room temperature and neutralized with 0.125M glycine for 5 minutes. Cells were then treated with Accutase and scraped. The preparation was centrifuged for 25 minutes at 15°C at 3500 rpm. The adipocytes, formed as a white layer (anti-pellet) at the meniscus, were harvested and snap-frozen in liquid nitrogen.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Frozen cells were resuspended in Qiagen RLT buffer and total RNA isolated using the Qiagen RNeasy Kit.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | cDNA was synthesized using 1-2 ug of high quality total RNA. Supercript II™ (Invitrogen) was used to reverse-transcribe the mRNA in the presence of a T7 containing oligo dT24 primer followed by second strand synthesis as recommended by Affymetrix. cDNA was purified using Agencourt RNAClean magnetic beads and used as a template for in vitro transcription using the GeneChip® One-Cycle Labeling Kit from Affymetrix. cRNA was purified using Agencourt magnetic beads and the quantity and purity of the cRNA was determined by absorbance at 260 nm and 260/280 absorbance ratio respectively. The quality of the cRNA was evaluated by assessing the size distribution of the cRNA using a 1.25% MOPS gel.
| | Sample_hyb_protocol | The labeled cRNA was fragmented as recommended by Affymetrix and 10 µg was added to a hybridization cocktail prior to loading onto individual HG-U133_Plus_2 GeneChip®. The microarrays were hybridized at 45°C for 16- 24 hours and washed and stained on an Affymetrix FS450 fluidics station according to manufacturer recommendations.
| | Sample_scan_protocol | The microarrays were scanned on a GeneChip® Scanner 3000.
| | Sample_data_processing | All of the array files were normalized using the Robust Multi-Array Average (RMA) method
| | Sample_platform_id | GPL570
| | Sample_contact_name | Shelley ,,des Etages
| | Sample_contact_email | shelley.g.desetages@pfizer.com
| | Sample_contact_institute | Pfizer Inc.
| | Sample_contact_address | Eastern Point Road
| | Sample_contact_city | Groton
| | Sample_contact_state | CT
| | Sample_contact_zip/postal_code | 06340
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM601nnn/GSM601622/suppl/GSM601622_Exp_131_3434_h2_mixed_no_TNF_no_insulin_2.CEL.gz
| | Sample_series_id | GSE24422
| | Sample_data_row_count | 54675
| | |
|
GSM601623 | GPL570 |
|
Mixed_Ins_rep 1
|
hMSC adipocytes, 5 hrs insulin
|
cell type: mixed adipocyte and stromal
treatment 2: 20 nM Insulin
|
|
| Sample_geo_accession | GSM601623
| | Sample_status | Public on Nov 30 2010
| | Sample_submission_date | Sep 28 2010
| | Sample_last_update_date | Sep 28 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | On Day 19 of differentiation, cells were treated with 0 or 10ng/ml TNF and incubated for 24hrs. Cells then underwent a 5 hr serum starvation followed by teatment with 0 or 20nM insulin for 2 hrs. At the end of the incubation, cells were harvested ( mixed) or fractioanted into adipocyte ( buiyant) and stromal (pelleted) fractions and subjected to RNA isolation.
| | Sample_growth_protocol_ch1 | Human mesenchymal stem cells were purchased from Lonza and cultured in basal media (Lonza PT3238) with mesenchymal stem cell growth supplement (Lonza PT4105). Cells were seeded at a density of 5000 cells/cm2. At confluence, the cells were differentiated into adipocytes using the adipogenic differentiation kit (Lonza PT3004). The total differentiation process took 19 days, with 14 days of induction and 5 days of maintenance. All media were from Lonza, except that the insulin concentration (in both induction and maintenance media) was adjusted to 120 pM. Mature adipocytes were subjected to 1% formaldehyde cross-linking for 10 minutes at room temperature and neutralized with 0.125M glycine for 5 minutes. Cells were then treated with Accutase and scraped. The preparation was centrifuged for 25 minutes at 15°C at 3500 rpm. The adipocytes, formed as a white layer (anti-pellet) at the meniscus, were harvested and snap-frozen in liquid nitrogen.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Frozen cells were resuspended in Qiagen RLT buffer and total RNA isolated using the Qiagen RNeasy Kit.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | cDNA was synthesized using 1-2 ug of high quality total RNA. Supercript II™ (Invitrogen) was used to reverse-transcribe the mRNA in the presence of a T7 containing oligo dT24 primer followed by second strand synthesis as recommended by Affymetrix. cDNA was purified using Agencourt RNAClean magnetic beads and used as a template for in vitro transcription using the GeneChip® One-Cycle Labeling Kit from Affymetrix. cRNA was purified using Agencourt magnetic beads and the quantity and purity of the cRNA was determined by absorbance at 260 nm and 260/280 absorbance ratio respectively. The quality of the cRNA was evaluated by assessing the size distribution of the cRNA using a 1.25% MOPS gel.
| | Sample_hyb_protocol | The labeled cRNA was fragmented as recommended by Affymetrix and 10 µg was added to a hybridization cocktail prior to loading onto individual HG-U133_Plus_2 GeneChip®. The microarrays were hybridized at 45°C for 16- 24 hours and washed and stained on an Affymetrix FS450 fluidics station according to manufacturer recommendations.
| | Sample_scan_protocol | The microarrays were scanned on a GeneChip® Scanner 3000.
| | Sample_data_processing | All of the array files were normalized using the Robust Multi-Array Average (RMA) method
| | Sample_platform_id | GPL570
| | Sample_contact_name | Shelley ,,des Etages
| | Sample_contact_email | shelley.g.desetages@pfizer.com
| | Sample_contact_institute | Pfizer Inc.
| | Sample_contact_address | Eastern Point Road
| | Sample_contact_city | Groton
| | Sample_contact_state | CT
| | Sample_contact_zip/postal_code | 06340
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM601nnn/GSM601623/suppl/GSM601623_Exp_131_3434_h2_mixed_no_TNF_20_nM_Insulin_3.CEL.gz
| | Sample_series_id | GSE24422
| | Sample_data_row_count | 54675
| | |
|
GSM601624 | GPL570 |
|
Mixed_Ins_rep 2
|
hMSC adipocytes, 5 hrs insulin
|
cell type: mixed adipocyte and stromal
treatment 2: 20 nM Insulin
|
|
| Sample_geo_accession | GSM601624
| | Sample_status | Public on Nov 30 2010
| | Sample_submission_date | Sep 28 2010
| | Sample_last_update_date | Sep 28 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | On Day 19 of differentiation, cells were treated with 0 or 10ng/ml TNF and incubated for 24hrs. Cells then underwent a 5 hr serum starvation followed by teatment with 0 or 20nM insulin for 2 hrs. At the end of the incubation, cells were harvested ( mixed) or fractioanted into adipocyte ( buiyant) and stromal (pelleted) fractions and subjected to RNA isolation.
| | Sample_growth_protocol_ch1 | Human mesenchymal stem cells were purchased from Lonza and cultured in basal media (Lonza PT3238) with mesenchymal stem cell growth supplement (Lonza PT4105). Cells were seeded at a density of 5000 cells/cm2. At confluence, the cells were differentiated into adipocytes using the adipogenic differentiation kit (Lonza PT3004). The total differentiation process took 19 days, with 14 days of induction and 5 days of maintenance. All media were from Lonza, except that the insulin concentration (in both induction and maintenance media) was adjusted to 120 pM. Mature adipocytes were subjected to 1% formaldehyde cross-linking for 10 minutes at room temperature and neutralized with 0.125M glycine for 5 minutes. Cells were then treated with Accutase and scraped. The preparation was centrifuged for 25 minutes at 15°C at 3500 rpm. The adipocytes, formed as a white layer (anti-pellet) at the meniscus, were harvested and snap-frozen in liquid nitrogen.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Frozen cells were resuspended in Qiagen RLT buffer and total RNA isolated using the Qiagen RNeasy Kit.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | cDNA was synthesized using 1-2 ug of high quality total RNA. Supercript II™ (Invitrogen) was used to reverse-transcribe the mRNA in the presence of a T7 containing oligo dT24 primer followed by second strand synthesis as recommended by Affymetrix. cDNA was purified using Agencourt RNAClean magnetic beads and used as a template for in vitro transcription using the GeneChip® One-Cycle Labeling Kit from Affymetrix. cRNA was purified using Agencourt magnetic beads and the quantity and purity of the cRNA was determined by absorbance at 260 nm and 260/280 absorbance ratio respectively. The quality of the cRNA was evaluated by assessing the size distribution of the cRNA using a 1.25% MOPS gel.
| | Sample_hyb_protocol | The labeled cRNA was fragmented as recommended by Affymetrix and 10 µg was added to a hybridization cocktail prior to loading onto individual HG-U133_Plus_2 GeneChip®. The microarrays were hybridized at 45°C for 16- 24 hours and washed and stained on an Affymetrix FS450 fluidics station according to manufacturer recommendations.
| | Sample_scan_protocol | The microarrays were scanned on a GeneChip® Scanner 3000.
| | Sample_data_processing | All of the array files were normalized using the Robust Multi-Array Average (RMA) method
| | Sample_platform_id | GPL570
| | Sample_contact_name | Shelley ,,des Etages
| | Sample_contact_email | shelley.g.desetages@pfizer.com
| | Sample_contact_institute | Pfizer Inc.
| | Sample_contact_address | Eastern Point Road
| | Sample_contact_city | Groton
| | Sample_contact_state | CT
| | Sample_contact_zip/postal_code | 06340
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM601nnn/GSM601624/suppl/GSM601624_Exp_131_3434_h2_mixed_no_TNF_20_nM_Insulin_4.CEL.gz
| | Sample_series_id | GSE24422
| | Sample_data_row_count | 54675
| | |
|
GSM601625 | GPL570 |
|
Mixed_TNF_rep 1
|
hMSC adipocytes, 24h TNF
|
cell type: mixed adipocyte and stromal
treatment 1: 10 ng/ml TNF
|
|
| Sample_geo_accession | GSM601625
| | Sample_status | Public on Nov 30 2010
| | Sample_submission_date | Sep 28 2010
| | Sample_last_update_date | Sep 28 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | On Day 19 of differentiation, cells were treated with 0 or 10ng/ml TNF and incubated for 24hrs. Cells then underwent a 5 hr serum starvation followed by teatment with 0 or 20nM insulin for 2 hrs. At the end of the incubation, cells were harvested ( mixed) or fractioanted into adipocyte ( buiyant) and stromal (pelleted) fractions and subjected to RNA isolation.
| | Sample_growth_protocol_ch1 | Human mesenchymal stem cells were purchased from Lonza and cultured in basal media (Lonza PT3238) with mesenchymal stem cell growth supplement (Lonza PT4105). Cells were seeded at a density of 5000 cells/cm2. At confluence, the cells were differentiated into adipocytes using the adipogenic differentiation kit (Lonza PT3004). The total differentiation process took 19 days, with 14 days of induction and 5 days of maintenance. All media were from Lonza, except that the insulin concentration (in both induction and maintenance media) was adjusted to 120 pM. Mature adipocytes were subjected to 1% formaldehyde cross-linking for 10 minutes at room temperature and neutralized with 0.125M glycine for 5 minutes. Cells were then treated with Accutase and scraped. The preparation was centrifuged for 25 minutes at 15°C at 3500 rpm. The adipocytes, formed as a white layer (anti-pellet) at the meniscus, were harvested and snap-frozen in liquid nitrogen.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Frozen cells were resuspended in Qiagen RLT buffer and total RNA isolated using the Qiagen RNeasy Kit.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | cDNA was synthesized using 1-2 ug of high quality total RNA. Supercript II™ (Invitrogen) was used to reverse-transcribe the mRNA in the presence of a T7 containing oligo dT24 primer followed by second strand synthesis as recommended by Affymetrix. cDNA was purified using Agencourt RNAClean magnetic beads and used as a template for in vitro transcription using the GeneChip® One-Cycle Labeling Kit from Affymetrix. cRNA was purified using Agencourt magnetic beads and the quantity and purity of the cRNA was determined by absorbance at 260 nm and 260/280 absorbance ratio respectively. The quality of the cRNA was evaluated by assessing the size distribution of the cRNA using a 1.25% MOPS gel.
| | Sample_hyb_protocol | The labeled cRNA was fragmented as recommended by Affymetrix and 10 µg was added to a hybridization cocktail prior to loading onto individual HG-U133_Plus_2 GeneChip®. The microarrays were hybridized at 45°C for 16- 24 hours and washed and stained on an Affymetrix FS450 fluidics station according to manufacturer recommendations.
| | Sample_scan_protocol | The microarrays were scanned on a GeneChip® Scanner 3000.
| | Sample_data_processing | All of the array files were normalized using the Robust Multi-Array Average (RMA) method
| | Sample_platform_id | GPL570
| | Sample_contact_name | Shelley ,,des Etages
| | Sample_contact_email | shelley.g.desetages@pfizer.com
| | Sample_contact_institute | Pfizer Inc.
| | Sample_contact_address | Eastern Point Road
| | Sample_contact_city | Groton
| | Sample_contact_state | CT
| | Sample_contact_zip/postal_code | 06340
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM601nnn/GSM601625/suppl/GSM601625_Exp_131_3434_h2_mixed_TNF_no_insulin_5.CEL.gz
| | Sample_series_id | GSE24422
| | Sample_data_row_count | 54675
| | |
|
GSM601626 | GPL570 |
|
Mixed_TNF_rep 2
|
hMSC adipocytes, 24h TNF
|
cell type: mixed adipocyte and stromal
treatment 1: 10 ng/ml TNF
|
|
| Sample_geo_accession | GSM601626
| | Sample_status | Public on Nov 30 2010
| | Sample_submission_date | Sep 28 2010
| | Sample_last_update_date | Sep 28 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | On Day 19 of differentiation, cells were treated with 0 or 10ng/ml TNF and incubated for 24hrs. Cells then underwent a 5 hr serum starvation followed by teatment with 0 or 20nM insulin for 2 hrs. At the end of the incubation, cells were harvested ( mixed) or fractioanted into adipocyte ( buiyant) and stromal (pelleted) fractions and subjected to RNA isolation.
| | Sample_growth_protocol_ch1 | Human mesenchymal stem cells were purchased from Lonza and cultured in basal media (Lonza PT3238) with mesenchymal stem cell growth supplement (Lonza PT4105). Cells were seeded at a density of 5000 cells/cm2. At confluence, the cells were differentiated into adipocytes using the adipogenic differentiation kit (Lonza PT3004). The total differentiation process took 19 days, with 14 days of induction and 5 days of maintenance. All media were from Lonza, except that the insulin concentration (in both induction and maintenance media) was adjusted to 120 pM. Mature adipocytes were subjected to 1% formaldehyde cross-linking for 10 minutes at room temperature and neutralized with 0.125M glycine for 5 minutes. Cells were then treated with Accutase and scraped. The preparation was centrifuged for 25 minutes at 15°C at 3500 rpm. The adipocytes, formed as a white layer (anti-pellet) at the meniscus, were harvested and snap-frozen in liquid nitrogen.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Frozen cells were resuspended in Qiagen RLT buffer and total RNA isolated using the Qiagen RNeasy Kit.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | cDNA was synthesized using 1-2 ug of high quality total RNA. Supercript II™ (Invitrogen) was used to reverse-transcribe the mRNA in the presence of a T7 containing oligo dT24 primer followed by second strand synthesis as recommended by Affymetrix. cDNA was purified using Agencourt RNAClean magnetic beads and used as a template for in vitro transcription using the GeneChip® One-Cycle Labeling Kit from Affymetrix. cRNA was purified using Agencourt magnetic beads and the quantity and purity of the cRNA was determined by absorbance at 260 nm and 260/280 absorbance ratio respectively. The quality of the cRNA was evaluated by assessing the size distribution of the cRNA using a 1.25% MOPS gel.
| | Sample_hyb_protocol | The labeled cRNA was fragmented as recommended by Affymetrix and 10 µg was added to a hybridization cocktail prior to loading onto individual HG-U133_Plus_2 GeneChip®. The microarrays were hybridized at 45°C for 16- 24 hours and washed and stained on an Affymetrix FS450 fluidics station according to manufacturer recommendations.
| | Sample_scan_protocol | The microarrays were scanned on a GeneChip® Scanner 3000.
| | Sample_data_processing | All of the array files were normalized using the Robust Multi-Array Average (RMA) method
| | Sample_platform_id | GPL570
| | Sample_contact_name | Shelley ,,des Etages
| | Sample_contact_email | shelley.g.desetages@pfizer.com
| | Sample_contact_institute | Pfizer Inc.
| | Sample_contact_address | Eastern Point Road
| | Sample_contact_city | Groton
| | Sample_contact_state | CT
| | Sample_contact_zip/postal_code | 06340
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM601nnn/GSM601626/suppl/GSM601626_Exp_131_3434_h2_mixed_TNF_no_insulin_6.CEL.gz
| | Sample_series_id | GSE24422
| | Sample_data_row_count | 54675
| | |
|
GSM601627 | GPL570 |
|
Mixed_TNF_Ins_rep 1
|
hMSC adipocytes, 24h TNF, 5 h insulin
|
cell type: mixed adipocyte and stromal
treatment 1: 10 ng/ml TNF
treatment 2: 20 nM Insulin
|
|
| Sample_geo_accession | GSM601627
| | Sample_status | Public on Nov 30 2010
| | Sample_submission_date | Sep 28 2010
| | Sample_last_update_date | Sep 28 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | On Day 19 of differentiation, cells were treated with 0 or 10ng/ml TNF and incubated for 24hrs. Cells then underwent a 5 hr serum starvation followed by teatment with 0 or 20nM insulin for 2 hrs. At the end of the incubation, cells were harvested ( mixed) or fractioanted into adipocyte ( buiyant) and stromal (pelleted) fractions and subjected to RNA isolation.
| | Sample_growth_protocol_ch1 | Human mesenchymal stem cells were purchased from Lonza and cultured in basal media (Lonza PT3238) with mesenchymal stem cell growth supplement (Lonza PT4105). Cells were seeded at a density of 5000 cells/cm2. At confluence, the cells were differentiated into adipocytes using the adipogenic differentiation kit (Lonza PT3004). The total differentiation process took 19 days, with 14 days of induction and 5 days of maintenance. All media were from Lonza, except that the insulin concentration (in both induction and maintenance media) was adjusted to 120 pM. Mature adipocytes were subjected to 1% formaldehyde cross-linking for 10 minutes at room temperature and neutralized with 0.125M glycine for 5 minutes. Cells were then treated with Accutase and scraped. The preparation was centrifuged for 25 minutes at 15°C at 3500 rpm. The adipocytes, formed as a white layer (anti-pellet) at the meniscus, were harvested and snap-frozen in liquid nitrogen.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Frozen cells were resuspended in Qiagen RLT buffer and total RNA isolated using the Qiagen RNeasy Kit.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | cDNA was synthesized using 1-2 ug of high quality total RNA. Supercript II™ (Invitrogen) was used to reverse-transcribe the mRNA in the presence of a T7 containing oligo dT24 primer followed by second strand synthesis as recommended by Affymetrix. cDNA was purified using Agencourt RNAClean magnetic beads and used as a template for in vitro transcription using the GeneChip® One-Cycle Labeling Kit from Affymetrix. cRNA was purified using Agencourt magnetic beads and the quantity and purity of the cRNA was determined by absorbance at 260 nm and 260/280 absorbance ratio respectively. The quality of the cRNA was evaluated by assessing the size distribution of the cRNA using a 1.25% MOPS gel.
| | Sample_hyb_protocol | The labeled cRNA was fragmented as recommended by Affymetrix and 10 µg was added to a hybridization cocktail prior to loading onto individual HG-U133_Plus_2 GeneChip®. The microarrays were hybridized at 45°C for 16- 24 hours and washed and stained on an Affymetrix FS450 fluidics station according to manufacturer recommendations.
| | Sample_scan_protocol | The microarrays were scanned on a GeneChip® Scanner 3000.
| | Sample_data_processing | All of the array files were normalized using the Robust Multi-Array Average (RMA) method
| | Sample_platform_id | GPL570
| | Sample_contact_name | Shelley ,,des Etages
| | Sample_contact_email | shelley.g.desetages@pfizer.com
| | Sample_contact_institute | Pfizer Inc.
| | Sample_contact_address | Eastern Point Road
| | Sample_contact_city | Groton
| | Sample_contact_state | CT
| | Sample_contact_zip/postal_code | 06340
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM601nnn/GSM601627/suppl/GSM601627_Exp_131_3434_h2_mixed_TNF_20nM_Insulin_7.CEL.gz
| | Sample_series_id | GSE24422
| | Sample_data_row_count | 54675
| | |
|
GSM601628 | GPL570 |
|
Mixed_TNF_Ins_rep 2
|
hMSC adipocytes, 24h TNF, 5 h insulin
|
cell type: mixed adipocyte and stromal
treatment 1: 10 ng/ml TNF
treatment 2: 20 nM Insulin
|
|
| Sample_geo_accession | GSM601628
| | Sample_status | Public on Nov 30 2010
| | Sample_submission_date | Sep 28 2010
| | Sample_last_update_date | Sep 28 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | On Day 19 of differentiation, cells were treated with 0 or 10ng/ml TNF and incubated for 24hrs. Cells then underwent a 5 hr serum starvation followed by teatment with 0 or 20nM insulin for 2 hrs. At the end of the incubation, cells were harvested ( mixed) or fractioanted into adipocyte ( buiyant) and stromal (pelleted) fractions and subjected to RNA isolation.
| | Sample_growth_protocol_ch1 | Human mesenchymal stem cells were purchased from Lonza and cultured in basal media (Lonza PT3238) with mesenchymal stem cell growth supplement (Lonza PT4105). Cells were seeded at a density of 5000 cells/cm2. At confluence, the cells were differentiated into adipocytes using the adipogenic differentiation kit (Lonza PT3004). The total differentiation process took 19 days, with 14 days of induction and 5 days of maintenance. All media were from Lonza, except that the insulin concentration (in both induction and maintenance media) was adjusted to 120 pM. Mature adipocytes were subjected to 1% formaldehyde cross-linking for 10 minutes at room temperature and neutralized with 0.125M glycine for 5 minutes. Cells were then treated with Accutase and scraped. The preparation was centrifuged for 25 minutes at 15°C at 3500 rpm. The adipocytes, formed as a white layer (anti-pellet) at the meniscus, were harvested and snap-frozen in liquid nitrogen.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Frozen cells were resuspended in Qiagen RLT buffer and total RNA isolated using the Qiagen RNeasy Kit.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | cDNA was synthesized using 1-2 ug of high quality total RNA. Supercript II™ (Invitrogen) was used to reverse-transcribe the mRNA in the presence of a T7 containing oligo dT24 primer followed by second strand synthesis as recommended by Affymetrix. cDNA was purified using Agencourt RNAClean magnetic beads and used as a template for in vitro transcription using the GeneChip® One-Cycle Labeling Kit from Affymetrix. cRNA was purified using Agencourt magnetic beads and the quantity and purity of the cRNA was determined by absorbance at 260 nm and 260/280 absorbance ratio respectively. The quality of the cRNA was evaluated by assessing the size distribution of the cRNA using a 1.25% MOPS gel.
| | Sample_hyb_protocol | The labeled cRNA was fragmented as recommended by Affymetrix and 10 µg was added to a hybridization cocktail prior to loading onto individual HG-U133_Plus_2 GeneChip®. The microarrays were hybridized at 45°C for 16- 24 hours and washed and stained on an Affymetrix FS450 fluidics station according to manufacturer recommendations.
| | Sample_scan_protocol | The microarrays were scanned on a GeneChip® Scanner 3000.
| | Sample_data_processing | All of the array files were normalized using the Robust Multi-Array Average (RMA) method
| | Sample_platform_id | GPL570
| | Sample_contact_name | Shelley ,,des Etages
| | Sample_contact_email | shelley.g.desetages@pfizer.com
| | Sample_contact_institute | Pfizer Inc.
| | Sample_contact_address | Eastern Point Road
| | Sample_contact_city | Groton
| | Sample_contact_state | CT
| | Sample_contact_zip/postal_code | 06340
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM601nnn/GSM601628/suppl/GSM601628_Exp_131_3434_h2_mixed_TNF_20nM_Insulin_8.CEL.gz
| | Sample_series_id | GSE24422
| | Sample_data_row_count | 54675
| | |
|
GSM601629 | GPL570 |
|
Adip_rep 1
|
hMSC adipocytes, buoyant fraction
|
cell type: Adipocyte
|
|
| Sample_geo_accession | GSM601629
| | Sample_status | Public on Nov 30 2010
| | Sample_submission_date | Sep 28 2010
| | Sample_last_update_date | Sep 28 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | On Day 19 of differentiation, cells were treated with 0 or 10ng/ml TNF and incubated for 24hrs. Cells then underwent a 5 hr serum starvation followed by teatment with 0 or 20nM insulin for 2 hrs. At the end of the incubation, cells were harvested ( mixed) or fractioanted into adipocyte ( buiyant) and stromal (pelleted) fractions and subjected to RNA isolation.
| | Sample_growth_protocol_ch1 | Human mesenchymal stem cells were purchased from Lonza and cultured in basal media (Lonza PT3238) with mesenchymal stem cell growth supplement (Lonza PT4105). Cells were seeded at a density of 5000 cells/cm2. At confluence, the cells were differentiated into adipocytes using the adipogenic differentiation kit (Lonza PT3004). The total differentiation process took 19 days, with 14 days of induction and 5 days of maintenance. All media were from Lonza, except that the insulin concentration (in both induction and maintenance media) was adjusted to 120 pM. Mature adipocytes were subjected to 1% formaldehyde cross-linking for 10 minutes at room temperature and neutralized with 0.125M glycine for 5 minutes. Cells were then treated with Accutase and scraped. The preparation was centrifuged for 25 minutes at 15°C at 3500 rpm. The adipocytes, formed as a white layer (anti-pellet) at the meniscus, were harvested and snap-frozen in liquid nitrogen.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Frozen cells were resuspended in Qiagen RLT buffer and total RNA isolated using the Qiagen RNeasy Kit.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | cDNA was synthesized using 1-2 ug of high quality total RNA. Supercript II™ (Invitrogen) was used to reverse-transcribe the mRNA in the presence of a T7 containing oligo dT24 primer followed by second strand synthesis as recommended by Affymetrix. cDNA was purified using Agencourt RNAClean magnetic beads and used as a template for in vitro transcription using the GeneChip® One-Cycle Labeling Kit from Affymetrix. cRNA was purified using Agencourt magnetic beads and the quantity and purity of the cRNA was determined by absorbance at 260 nm and 260/280 absorbance ratio respectively. The quality of the cRNA was evaluated by assessing the size distribution of the cRNA using a 1.25% MOPS gel.
| | Sample_hyb_protocol | The labeled cRNA was fragmented as recommended by Affymetrix and 10 µg was added to a hybridization cocktail prior to loading onto individual HG-U133_Plus_2 GeneChip®. The microarrays were hybridized at 45°C for 16- 24 hours and washed and stained on an Affymetrix FS450 fluidics station according to manufacturer recommendations.
| | Sample_scan_protocol | The microarrays were scanned on a GeneChip® Scanner 3000.
| | Sample_data_processing | All of the array files were normalized using the Robust Multi-Array Average (RMA) method
| | Sample_platform_id | GPL570
| | Sample_contact_name | Shelley ,,des Etages
| | Sample_contact_email | shelley.g.desetages@pfizer.com
| | Sample_contact_institute | Pfizer Inc.
| | Sample_contact_address | Eastern Point Road
| | Sample_contact_city | Groton
| | Sample_contact_state | CT
| | Sample_contact_zip/postal_code | 06340
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM601nnn/GSM601629/suppl/GSM601629_Exp_131_3434_h2_adipocyte_no_TNF_no_insulin_9.CEL.gz
| | Sample_series_id | GSE24422
| | Sample_data_row_count | 54675
| | |
|
GSM601630 | GPL570 |
|
Adip_rep 2
|
hMSC adipocytes, buoyant fraction
|
cell type: Adipocyte
|
|
| Sample_geo_accession | GSM601630
| | Sample_status | Public on Nov 30 2010
| | Sample_submission_date | Sep 28 2010
| | Sample_last_update_date | Sep 28 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | On Day 19 of differentiation, cells were treated with 0 or 10ng/ml TNF and incubated for 24hrs. Cells then underwent a 5 hr serum starvation followed by teatment with 0 or 20nM insulin for 2 hrs. At the end of the incubation, cells were harvested ( mixed) or fractioanted into adipocyte ( buiyant) and stromal (pelleted) fractions and subjected to RNA isolation.
| | Sample_growth_protocol_ch1 | Human mesenchymal stem cells were purchased from Lonza and cultured in basal media (Lonza PT3238) with mesenchymal stem cell growth supplement (Lonza PT4105). Cells were seeded at a density of 5000 cells/cm2. At confluence, the cells were differentiated into adipocytes using the adipogenic differentiation kit (Lonza PT3004). The total differentiation process took 19 days, with 14 days of induction and 5 days of maintenance. All media were from Lonza, except that the insulin concentration (in both induction and maintenance media) was adjusted to 120 pM. Mature adipocytes were subjected to 1% formaldehyde cross-linking for 10 minutes at room temperature and neutralized with 0.125M glycine for 5 minutes. Cells were then treated with Accutase and scraped. The preparation was centrifuged for 25 minutes at 15°C at 3500 rpm. The adipocytes, formed as a white layer (anti-pellet) at the meniscus, were harvested and snap-frozen in liquid nitrogen.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Frozen cells were resuspended in Qiagen RLT buffer and total RNA isolated using the Qiagen RNeasy Kit.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | cDNA was synthesized using 1-2 ug of high quality total RNA. Supercript II™ (Invitrogen) was used to reverse-transcribe the mRNA in the presence of a T7 containing oligo dT24 primer followed by second strand synthesis as recommended by Affymetrix. cDNA was purified using Agencourt RNAClean magnetic beads and used as a template for in vitro transcription using the GeneChip® One-Cycle Labeling Kit from Affymetrix. cRNA was purified using Agencourt magnetic beads and the quantity and purity of the cRNA was determined by absorbance at 260 nm and 260/280 absorbance ratio respectively. The quality of the cRNA was evaluated by assessing the size distribution of the cRNA using a 1.25% MOPS gel.
| | Sample_hyb_protocol | The labeled cRNA was fragmented as recommended by Affymetrix and 10 µg was added to a hybridization cocktail prior to loading onto individual HG-U133_Plus_2 GeneChip®. The microarrays were hybridized at 45°C for 16- 24 hours and washed and stained on an Affymetrix FS450 fluidics station according to manufacturer recommendations.
| | Sample_scan_protocol | The microarrays were scanned on a GeneChip® Scanner 3000.
| | Sample_data_processing | All of the array files were normalized using the Robust Multi-Array Average (RMA) method
| | Sample_platform_id | GPL570
| | Sample_contact_name | Shelley ,,des Etages
| | Sample_contact_email | shelley.g.desetages@pfizer.com
| | Sample_contact_institute | Pfizer Inc.
| | Sample_contact_address | Eastern Point Road
| | Sample_contact_city | Groton
| | Sample_contact_state | CT
| | Sample_contact_zip/postal_code | 06340
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM601nnn/GSM601630/suppl/GSM601630_Exp_131_3434_h2_adipocyte_no_TNF_no_insulin_10.CEL.gz
| | Sample_series_id | GSE24422
| | Sample_data_row_count | 54675
| | |
|
GSM601631 | GPL570 |
|
Adip_Ins_rep1
|
hMSC adipocytes, 5 hrs insulin, buoyant fraction
|
cell type: Adipocyte
treatment 2: 20 nM Insulin
|
|
| Sample_geo_accession | GSM601631
| | Sample_status | Public on Nov 30 2010
| | Sample_submission_date | Sep 28 2010
| | Sample_last_update_date | Sep 28 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | On Day 19 of differentiation, cells were treated with 0 or 10ng/ml TNF and incubated for 24hrs. Cells then underwent a 5 hr serum starvation followed by teatment with 0 or 20nM insulin for 2 hrs. At the end of the incubation, cells were harvested ( mixed) or fractioanted into adipocyte ( buiyant) and stromal (pelleted) fractions and subjected to RNA isolation.
| | Sample_growth_protocol_ch1 | Human mesenchymal stem cells were purchased from Lonza and cultured in basal media (Lonza PT3238) with mesenchymal stem cell growth supplement (Lonza PT4105). Cells were seeded at a density of 5000 cells/cm2. At confluence, the cells were differentiated into adipocytes using the adipogenic differentiation kit (Lonza PT3004). The total differentiation process took 19 days, with 14 days of induction and 5 days of maintenance. All media were from Lonza, except that the insulin concentration (in both induction and maintenance media) was adjusted to 120 pM. Mature adipocytes were subjected to 1% formaldehyde cross-linking for 10 minutes at room temperature and neutralized with 0.125M glycine for 5 minutes. Cells were then treated with Accutase and scraped. The preparation was centrifuged for 25 minutes at 15°C at 3500 rpm. The adipocytes, formed as a white layer (anti-pellet) at the meniscus, were harvested and snap-frozen in liquid nitrogen.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Frozen cells were resuspended in Qiagen RLT buffer and total RNA isolated using the Qiagen RNeasy Kit.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | cDNA was synthesized using 1-2 ug of high quality total RNA. Supercript II™ (Invitrogen) was used to reverse-transcribe the mRNA in the presence of a T7 containing oligo dT24 primer followed by second strand synthesis as recommended by Affymetrix. cDNA was purified using Agencourt RNAClean magnetic beads and used as a template for in vitro transcription using the GeneChip® One-Cycle Labeling Kit from Affymetrix. cRNA was purified using Agencourt magnetic beads and the quantity and purity of the cRNA was determined by absorbance at 260 nm and 260/280 absorbance ratio respectively. The quality of the cRNA was evaluated by assessing the size distribution of the cRNA using a 1.25% MOPS gel.
| | Sample_hyb_protocol | The labeled cRNA was fragmented as recommended by Affymetrix and 10 µg was added to a hybridization cocktail prior to loading onto individual HG-U133_Plus_2 GeneChip®. The microarrays were hybridized at 45°C for 16- 24 hours and washed and stained on an Affymetrix FS450 fluidics station according to manufacturer recommendations.
| | Sample_scan_protocol | The microarrays were scanned on a GeneChip® Scanner 3000.
| | Sample_data_processing | All of the array files were normalized using the Robust Multi-Array Average (RMA) method
| | Sample_platform_id | GPL570
| | Sample_contact_name | Shelley ,,des Etages
| | Sample_contact_email | shelley.g.desetages@pfizer.com
| | Sample_contact_institute | Pfizer Inc.
| | Sample_contact_address | Eastern Point Road
| | Sample_contact_city | Groton
| | Sample_contact_state | CT
| | Sample_contact_zip/postal_code | 06340
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM601nnn/GSM601631/suppl/GSM601631_Exp_131_3434_h2_adipocyte_no_TNF_20_nM_Insulin_11.CEL.gz
| | Sample_series_id | GSE24422
| | Sample_data_row_count | 54675
| | |
|
GSM601632 | GPL570 |
|
Adip_Ins_rep 2
|
hMSC adipocytes, 5 hrs insulin, buoyant fraction
|
cell type: Adipocyte
treatment 2: 20 nM Insulin
|
|
| Sample_geo_accession | GSM601632
| | Sample_status | Public on Nov 30 2010
| | Sample_submission_date | Sep 28 2010
| | Sample_last_update_date | Sep 28 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | On Day 19 of differentiation, cells were treated with 0 or 10ng/ml TNF and incubated for 24hrs. Cells then underwent a 5 hr serum starvation followed by teatment with 0 or 20nM insulin for 2 hrs. At the end of the incubation, cells were harvested ( mixed) or fractioanted into adipocyte ( buiyant) and stromal (pelleted) fractions and subjected to RNA isolation.
| | Sample_growth_protocol_ch1 | Human mesenchymal stem cells were purchased from Lonza and cultured in basal media (Lonza PT3238) with mesenchymal stem cell growth supplement (Lonza PT4105). Cells were seeded at a density of 5000 cells/cm2. At confluence, the cells were differentiated into adipocytes using the adipogenic differentiation kit (Lonza PT3004). The total differentiation process took 19 days, with 14 days of induction and 5 days of maintenance. All media were from Lonza, except that the insulin concentration (in both induction and maintenance media) was adjusted to 120 pM. Mature adipocytes were subjected to 1% formaldehyde cross-linking for 10 minutes at room temperature and neutralized with 0.125M glycine for 5 minutes. Cells were then treated with Accutase and scraped. The preparation was centrifuged for 25 minutes at 15°C at 3500 rpm. The adipocytes, formed as a white layer (anti-pellet) at the meniscus, were harvested and snap-frozen in liquid nitrogen.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Frozen cells were resuspended in Qiagen RLT buffer and total RNA isolated using the Qiagen RNeasy Kit.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | cDNA was synthesized using 1-2 ug of high quality total RNA. Supercript II™ (Invitrogen) was used to reverse-transcribe the mRNA in the presence of a T7 containing oligo dT24 primer followed by second strand synthesis as recommended by Affymetrix. cDNA was purified using Agencourt RNAClean magnetic beads and used as a template for in vitro transcription using the GeneChip® One-Cycle Labeling Kit from Affymetrix. cRNA was purified using Agencourt magnetic beads and the quantity and purity of the cRNA was determined by absorbance at 260 nm and 260/280 absorbance ratio respectively. The quality of the cRNA was evaluated by assessing the size distribution of the cRNA using a 1.25% MOPS gel.
| | Sample_hyb_protocol | The labeled cRNA was fragmented as recommended by Affymetrix and 10 µg was added to a hybridization cocktail prior to loading onto individual HG-U133_Plus_2 GeneChip®. The microarrays were hybridized at 45°C for 16- 24 hours and washed and stained on an Affymetrix FS450 fluidics station according to manufacturer recommendations.
| | Sample_scan_protocol | The microarrays were scanned on a GeneChip® Scanner 3000.
| | Sample_data_processing | All of the array files were normalized using the Robust Multi-Array Average (RMA) method
| | Sample_platform_id | GPL570
| | Sample_contact_name | Shelley ,,des Etages
| | Sample_contact_email | shelley.g.desetages@pfizer.com
| | Sample_contact_institute | Pfizer Inc.
| | Sample_contact_address | Eastern Point Road
| | Sample_contact_city | Groton
| | Sample_contact_state | CT
| | Sample_contact_zip/postal_code | 06340
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM601nnn/GSM601632/suppl/GSM601632_Exp_131_3434_h2_adipocyte_no_TNF_20_nM_Insulin_12.CEL.gz
| | Sample_series_id | GSE24422
| | Sample_data_row_count | 54675
| | |
|
GSM601633 | GPL570 |
|
Adip_TNF_rep 1
|
hMSC adipocytes, 24 hrs TNF, buoyant fraction
|
cell type: Adipocyte
treatment 1: 10 ng/ml TNF
|
|
| Sample_geo_accession | GSM601633
| | Sample_status | Public on Nov 30 2010
| | Sample_submission_date | Sep 28 2010
| | Sample_last_update_date | Sep 28 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | On Day 19 of differentiation, cells were treated with 0 or 10ng/ml TNF and incubated for 24hrs. Cells then underwent a 5 hr serum starvation followed by teatment with 0 or 20nM insulin for 2 hrs. At the end of the incubation, cells were harvested ( mixed) or fractioanted into adipocyte ( buiyant) and stromal (pelleted) fractions and subjected to RNA isolation.
| | Sample_growth_protocol_ch1 | Human mesenchymal stem cells were purchased from Lonza and cultured in basal media (Lonza PT3238) with mesenchymal stem cell growth supplement (Lonza PT4105). Cells were seeded at a density of 5000 cells/cm2. At confluence, the cells were differentiated into adipocytes using the adipogenic differentiation kit (Lonza PT3004). The total differentiation process took 19 days, with 14 days of induction and 5 days of maintenance. All media were from Lonza, except that the insulin concentration (in both induction and maintenance media) was adjusted to 120 pM. Mature adipocytes were subjected to 1% formaldehyde cross-linking for 10 minutes at room temperature and neutralized with 0.125M glycine for 5 minutes. Cells were then treated with Accutase and scraped. The preparation was centrifuged for 25 minutes at 15°C at 3500 rpm. The adipocytes, formed as a white layer (anti-pellet) at the meniscus, were harvested and snap-frozen in liquid nitrogen.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Frozen cells were resuspended in Qiagen RLT buffer and total RNA isolated using the Qiagen RNeasy Kit.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | cDNA was synthesized using 1-2 ug of high quality total RNA. Supercript II™ (Invitrogen) was used to reverse-transcribe the mRNA in the presence of a T7 containing oligo dT24 primer followed by second strand synthesis as recommended by Affymetrix. cDNA was purified using Agencourt RNAClean magnetic beads and used as a template for in vitro transcription using the GeneChip® One-Cycle Labeling Kit from Affymetrix. cRNA was purified using Agencourt magnetic beads and the quantity and purity of the cRNA was determined by absorbance at 260 nm and 260/280 absorbance ratio respectively. The quality of the cRNA was evaluated by assessing the size distribution of the cRNA using a 1.25% MOPS gel.
| | Sample_hyb_protocol | The labeled cRNA was fragmented as recommended by Affymetrix and 10 µg was added to a hybridization cocktail prior to loading onto individual HG-U133_Plus_2 GeneChip®. The microarrays were hybridized at 45°C for 16- 24 hours and washed and stained on an Affymetrix FS450 fluidics station according to manufacturer recommendations.
| | Sample_scan_protocol | The microarrays were scanned on a GeneChip® Scanner 3000.
| | Sample_data_processing | All of the array files were normalized using the Robust Multi-Array Average (RMA) method
| | Sample_platform_id | GPL570
| | Sample_contact_name | Shelley ,,des Etages
| | Sample_contact_email | shelley.g.desetages@pfizer.com
| | Sample_contact_institute | Pfizer Inc.
| | Sample_contact_address | Eastern Point Road
| | Sample_contact_city | Groton
| | Sample_contact_state | CT
| | Sample_contact_zip/postal_code | 06340
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM601nnn/GSM601633/suppl/GSM601633_Exp_131_3434_h2_adipocyte_TNF_no_insulin_13.CEL.gz
| | Sample_series_id | GSE24422
| | Sample_data_row_count | 54675
| | |
|
GSM601634 | GPL570 |
|
Adip_TNF_rep 2
|
hMSC adipocytes, 24 hrs TNF, buoyant fraction
|
cell type: Adipocyte
treatment 1: 10 ng/ml TNF
|
|
| Sample_geo_accession | GSM601634
| | Sample_status | Public on Nov 30 2010
| | Sample_submission_date | Sep 28 2010
| | Sample_last_update_date | Sep 28 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | On Day 19 of differentiation, cells were treated with 0 or 10ng/ml TNF and incubated for 24hrs. Cells then underwent a 5 hr serum starvation followed by teatment with 0 or 20nM insulin for 2 hrs. At the end of the incubation, cells were harvested ( mixed) or fractioanted into adipocyte ( buiyant) and stromal (pelleted) fractions and subjected to RNA isolation.
| | Sample_growth_protocol_ch1 | Human mesenchymal stem cells were purchased from Lonza and cultured in basal media (Lonza PT3238) with mesenchymal stem cell growth supplement (Lonza PT4105). Cells were seeded at a density of 5000 cells/cm2. At confluence, the cells were differentiated into adipocytes using the adipogenic differentiation kit (Lonza PT3004). The total differentiation process took 19 days, with 14 days of induction and 5 days of maintenance. All media were from Lonza, except that the insulin concentration (in both induction and maintenance media) was adjusted to 120 pM. Mature adipocytes were subjected to 1% formaldehyde cross-linking for 10 minutes at room temperature and neutralized with 0.125M glycine for 5 minutes. Cells were then treated with Accutase and scraped. The preparation was centrifuged for 25 minutes at 15°C at 3500 rpm. The adipocytes, formed as a white layer (anti-pellet) at the meniscus, were harvested and snap-frozen in liquid nitrogen.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Frozen cells were resuspended in Qiagen RLT buffer and total RNA isolated using the Qiagen RNeasy Kit.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | cDNA was synthesized using 1-2 ug of high quality total RNA. Supercript II™ (Invitrogen) was used to reverse-transcribe the mRNA in the presence of a T7 containing oligo dT24 primer followed by second strand synthesis as recommended by Affymetrix. cDNA was purified using Agencourt RNAClean magnetic beads and used as a template for in vitro transcription using the GeneChip® One-Cycle Labeling Kit from Affymetrix. cRNA was purified using Agencourt magnetic beads and the quantity and purity of the cRNA was determined by absorbance at 260 nm and 260/280 absorbance ratio respectively. The quality of the cRNA was evaluated by assessing the size distribution of the cRNA using a 1.25% MOPS gel.
| | Sample_hyb_protocol | The labeled cRNA was fragmented as recommended by Affymetrix and 10 µg was added to a hybridization cocktail prior to loading onto individual HG-U133_Plus_2 GeneChip®. The microarrays were hybridized at 45°C for 16- 24 hours and washed and stained on an Affymetrix FS450 fluidics station according to manufacturer recommendations.
| | Sample_scan_protocol | The microarrays were scanned on a GeneChip® Scanner 3000.
| | Sample_data_processing | All of the array files were normalized using the Robust Multi-Array Average (RMA) method
| | Sample_platform_id | GPL570
| | Sample_contact_name | Shelley ,,des Etages
| | Sample_contact_email | shelley.g.desetages@pfizer.com
| | Sample_contact_institute | Pfizer Inc.
| | Sample_contact_address | Eastern Point Road
| | Sample_contact_city | Groton
| | Sample_contact_state | CT
| | Sample_contact_zip/postal_code | 06340
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM601nnn/GSM601634/suppl/GSM601634_Exp_131_3434_h2_adipocyte_TNF_no_insulin_14.CEL.gz
| | Sample_series_id | GSE24422
| | Sample_data_row_count | 54675
| | |
|
GSM601635 | GPL570 |
|
Adip_TNF_Ins_rep 1
|
hMSC adipocytes, 24h TNF, 5 h insulin, buoyant fraction
|
cell type: Adipocyte
treatment 1: 10 ng/ml TNF
treatment 2: 20 nM Insulin
|
|
| Sample_geo_accession | GSM601635
| | Sample_status | Public on Nov 30 2010
| | Sample_submission_date | Sep 28 2010
| | Sample_last_update_date | Sep 28 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | On Day 19 of differentiation, cells were treated with 0 or 10ng/ml TNF and incubated for 24hrs. Cells then underwent a 5 hr serum starvation followed by teatment with 0 or 20nM insulin for 2 hrs. At the end of the incubation, cells were harvested ( mixed) or fractioanted into adipocyte ( buiyant) and stromal (pelleted) fractions and subjected to RNA isolation.
| | Sample_growth_protocol_ch1 | Human mesenchymal stem cells were purchased from Lonza and cultured in basal media (Lonza PT3238) with mesenchymal stem cell growth supplement (Lonza PT4105). Cells were seeded at a density of 5000 cells/cm2. At confluence, the cells were differentiated into adipocytes using the adipogenic differentiation kit (Lonza PT3004). The total differentiation process took 19 days, with 14 days of induction and 5 days of maintenance. All media were from Lonza, except that the insulin concentration (in both induction and maintenance media) was adjusted to 120 pM. Mature adipocytes were subjected to 1% formaldehyde cross-linking for 10 minutes at room temperature and neutralized with 0.125M glycine for 5 minutes. Cells were then treated with Accutase and scraped. The preparation was centrifuged for 25 minutes at 15°C at 3500 rpm. The adipocytes, formed as a white layer (anti-pellet) at the meniscus, were harvested and snap-frozen in liquid nitrogen.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Frozen cells were resuspended in Qiagen RLT buffer and total RNA isolated using the Qiagen RNeasy Kit.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | cDNA was synthesized using 1-2 ug of high quality total RNA. Supercript II™ (Invitrogen) was used to reverse-transcribe the mRNA in the presence of a T7 containing oligo dT24 primer followed by second strand synthesis as recommended by Affymetrix. cDNA was purified using Agencourt RNAClean magnetic beads and used as a template for in vitro transcription using the GeneChip® One-Cycle Labeling Kit from Affymetrix. cRNA was purified using Agencourt magnetic beads and the quantity and purity of the cRNA was determined by absorbance at 260 nm and 260/280 absorbance ratio respectively. The quality of the cRNA was evaluated by assessing the size distribution of the cRNA using a 1.25% MOPS gel.
| | Sample_hyb_protocol | The labeled cRNA was fragmented as recommended by Affymetrix and 10 µg was added to a hybridization cocktail prior to loading onto individual HG-U133_Plus_2 GeneChip®. The microarrays were hybridized at 45°C for 16- 24 hours and washed and stained on an Affymetrix FS450 fluidics station according to manufacturer recommendations.
| | Sample_scan_protocol | The microarrays were scanned on a GeneChip® Scanner 3000.
| | Sample_data_processing | All of the array files were normalized using the Robust Multi-Array Average (RMA) method
| | Sample_platform_id | GPL570
| | Sample_contact_name | Shelley ,,des Etages
| | Sample_contact_email | shelley.g.desetages@pfizer.com
| | Sample_contact_institute | Pfizer Inc.
| | Sample_contact_address | Eastern Point Road
| | Sample_contact_city | Groton
| | Sample_contact_state | CT
| | Sample_contact_zip/postal_code | 06340
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM601nnn/GSM601635/suppl/GSM601635_Exp_131_3434_h2_adipocyte_TNF_20nM_Insulin_15.CEL.gz
| | Sample_series_id | GSE24422
| | Sample_data_row_count | 54675
| | |
|
GSM601636 | GPL570 |
|
Adip_TNF_Ins_rep 2
|
hMSC adipocytes, 24h TNF, 5 h insulin, buoyant fraction
|
cell type: Adipocyte
treatment 1: 10 ng/ml TNF
treatment 2: 20 nM Insulin
|
|
| Sample_geo_accession | GSM601636
| | Sample_status | Public on Nov 30 2010
| | Sample_submission_date | Sep 28 2010
| | Sample_last_update_date | Sep 28 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | On Day 19 of differentiation, cells were treated with 0 or 10ng/ml TNF and incubated for 24hrs. Cells then underwent a 5 hr serum starvation followed by teatment with 0 or 20nM insulin for 2 hrs. At the end of the incubation, cells were harvested ( mixed) or fractioanted into adipocyte ( buiyant) and stromal (pelleted) fractions and subjected to RNA isolation.
| | Sample_growth_protocol_ch1 | Human mesenchymal stem cells were purchased from Lonza and cultured in basal media (Lonza PT3238) with mesenchymal stem cell growth supplement (Lonza PT4105). Cells were seeded at a density of 5000 cells/cm2. At confluence, the cells were differentiated into adipocytes using the adipogenic differentiation kit (Lonza PT3004). The total differentiation process took 19 days, with 14 days of induction and 5 days of maintenance. All media were from Lonza, except that the insulin concentration (in both induction and maintenance media) was adjusted to 120 pM. Mature adipocytes were subjected to 1% formaldehyde cross-linking for 10 minutes at room temperature and neutralized with 0.125M glycine for 5 minutes. Cells were then treated with Accutase and scraped. The preparation was centrifuged for 25 minutes at 15°C at 3500 rpm. The adipocytes, formed as a white layer (anti-pellet) at the meniscus, were harvested and snap-frozen in liquid nitrogen.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Frozen cells were resuspended in Qiagen RLT buffer and total RNA isolated using the Qiagen RNeasy Kit.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | cDNA was synthesized using 1-2 ug of high quality total RNA. Supercript II™ (Invitrogen) was used to reverse-transcribe the mRNA in the presence of a T7 containing oligo dT24 primer followed by second strand synthesis as recommended by Affymetrix. cDNA was purified using Agencourt RNAClean magnetic beads and used as a template for in vitro transcription using the GeneChip® One-Cycle Labeling Kit from Affymetrix. cRNA was purified using Agencourt magnetic beads and the quantity and purity of the cRNA was determined by absorbance at 260 nm and 260/280 absorbance ratio respectively. The quality of the cRNA was evaluated by assessing the size distribution of the cRNA using a 1.25% MOPS gel.
| | Sample_hyb_protocol | The labeled cRNA was fragmented as recommended by Affymetrix and 10 µg was added to a hybridization cocktail prior to loading onto individual HG-U133_Plus_2 GeneChip®. The microarrays were hybridized at 45°C for 16- 24 hours and washed and stained on an Affymetrix FS450 fluidics station according to manufacturer recommendations.
| | Sample_scan_protocol | The microarrays were scanned on a GeneChip® Scanner 3000.
| | Sample_data_processing | All of the array files were normalized using the Robust Multi-Array Average (RMA) method
| | Sample_platform_id | GPL570
| | Sample_contact_name | Shelley ,,des Etages
| | Sample_contact_email | shelley.g.desetages@pfizer.com
| | Sample_contact_institute | Pfizer Inc.
| | Sample_contact_address | Eastern Point Road
| | Sample_contact_city | Groton
| | Sample_contact_state | CT
| | Sample_contact_zip/postal_code | 06340
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM601nnn/GSM601636/suppl/GSM601636_Exp_131_3434_h2_adipocyte_TNF_20nM_Insulin_16.CEL.gz
| | Sample_series_id | GSE24422
| | Sample_data_row_count | 54675
| | |
|
GSM601637 | GPL570 |
|
Strom_rep 1
|
hMSC adipocytes, pelleted fraction
|
cell type: Stromal
|
|
| Sample_geo_accession | GSM601637
| | Sample_status | Public on Nov 30 2010
| | Sample_submission_date | Sep 28 2010
| | Sample_last_update_date | Sep 28 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | On Day 19 of differentiation, cells were treated with 0 or 10ng/ml TNF and incubated for 24hrs. Cells then underwent a 5 hr serum starvation followed by teatment with 0 or 20nM insulin for 2 hrs. At the end of the incubation, cells were harvested ( mixed) or fractioanted into adipocyte ( buiyant) and stromal (pelleted) fractions and subjected to RNA isolation.
| | Sample_growth_protocol_ch1 | Human mesenchymal stem cells were purchased from Lonza and cultured in basal media (Lonza PT3238) with mesenchymal stem cell growth supplement (Lonza PT4105). Cells were seeded at a density of 5000 cells/cm2. At confluence, the cells were differentiated into adipocytes using the adipogenic differentiation kit (Lonza PT3004). The total differentiation process took 19 days, with 14 days of induction and 5 days of maintenance. All media were from Lonza, except that the insulin concentration (in both induction and maintenance media) was adjusted to 120 pM. Mature adipocytes were subjected to 1% formaldehyde cross-linking for 10 minutes at room temperature and neutralized with 0.125M glycine for 5 minutes. Cells were then treated with Accutase and scraped. The preparation was centrifuged for 25 minutes at 15°C at 3500 rpm. The adipocytes, formed as a white layer (anti-pellet) at the meniscus, were harvested and snap-frozen in liquid nitrogen.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Frozen cells were resuspended in Qiagen RLT buffer and total RNA isolated using the Qiagen RNeasy Kit.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | cDNA was synthesized using 1-2 ug of high quality total RNA. Supercript II™ (Invitrogen) was used to reverse-transcribe the mRNA in the presence of a T7 containing oligo dT24 primer followed by second strand synthesis as recommended by Affymetrix. cDNA was purified using Agencourt RNAClean magnetic beads and used as a template for in vitro transcription using the GeneChip® One-Cycle Labeling Kit from Affymetrix. cRNA was purified using Agencourt magnetic beads and the quantity and purity of the cRNA was determined by absorbance at 260 nm and 260/280 absorbance ratio respectively. The quality of the cRNA was evaluated by assessing the size distribution of the cRNA using a 1.25% MOPS gel.
| | Sample_hyb_protocol | The labeled cRNA was fragmented as recommended by Affymetrix and 10 µg was added to a hybridization cocktail prior to loading onto individual HG-U133_Plus_2 GeneChip®. The microarrays were hybridized at 45°C for 16- 24 hours and washed and stained on an Affymetrix FS450 fluidics station according to manufacturer recommendations.
| | Sample_scan_protocol | The microarrays were scanned on a GeneChip® Scanner 3000.
| | Sample_data_processing | All of the array files were normalized using the Robust Multi-Array Average (RMA) method
| | Sample_platform_id | GPL570
| | Sample_contact_name | Shelley ,,des Etages
| | Sample_contact_email | shelley.g.desetages@pfizer.com
| | Sample_contact_institute | Pfizer Inc.
| | Sample_contact_address | Eastern Point Road
| | Sample_contact_city | Groton
| | Sample_contact_state | CT
| | Sample_contact_zip/postal_code | 06340
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM601nnn/GSM601637/suppl/GSM601637_Exp_131_3434_h2_stromal_no_TNF_no_insulin_17.CEL.gz
| | Sample_series_id | GSE24422
| | Sample_data_row_count | 54675
| | |
|
GSM601638 | GPL570 |
|
Strom_rep 2
|
hMSC adipocytes, pelleted fraction
|
cell type: Stromal
|
|
| Sample_geo_accession | GSM601638
| | Sample_status | Public on Nov 30 2010
| | Sample_submission_date | Sep 28 2010
| | Sample_last_update_date | Sep 28 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | On Day 19 of differentiation, cells were treated with 0 or 10ng/ml TNF and incubated for 24hrs. Cells then underwent a 5 hr serum starvation followed by teatment with 0 or 20nM insulin for 2 hrs. At the end of the incubation, cells were harvested ( mixed) or fractioanted into adipocyte ( buiyant) and stromal (pelleted) fractions and subjected to RNA isolation.
| | Sample_growth_protocol_ch1 | Human mesenchymal stem cells were purchased from Lonza and cultured in basal media (Lonza PT3238) with mesenchymal stem cell growth supplement (Lonza PT4105). Cells were seeded at a density of 5000 cells/cm2. At confluence, the cells were differentiated into adipocytes using the adipogenic differentiation kit (Lonza PT3004). The total differentiation process took 19 days, with 14 days of induction and 5 days of maintenance. All media were from Lonza, except that the insulin concentration (in both induction and maintenance media) was adjusted to 120 pM. Mature adipocytes were subjected to 1% formaldehyde cross-linking for 10 minutes at room temperature and neutralized with 0.125M glycine for 5 minutes. Cells were then treated with Accutase and scraped. The preparation was centrifuged for 25 minutes at 15°C at 3500 rpm. The adipocytes, formed as a white layer (anti-pellet) at the meniscus, were harvested and snap-frozen in liquid nitrogen.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Frozen cells were resuspended in Qiagen RLT buffer and total RNA isolated using the Qiagen RNeasy Kit.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | cDNA was synthesized using 1-2 ug of high quality total RNA. Supercript II™ (Invitrogen) was used to reverse-transcribe the mRNA in the presence of a T7 containing oligo dT24 primer followed by second strand synthesis as recommended by Affymetrix. cDNA was purified using Agencourt RNAClean magnetic beads and used as a template for in vitro transcription using the GeneChip® One-Cycle Labeling Kit from Affymetrix. cRNA was purified using Agencourt magnetic beads and the quantity and purity of the cRNA was determined by absorbance at 260 nm and 260/280 absorbance ratio respectively. The quality of the cRNA was evaluated by assessing the size distribution of the cRNA using a 1.25% MOPS gel.
| | Sample_hyb_protocol | The labeled cRNA was fragmented as recommended by Affymetrix and 10 µg was added to a hybridization cocktail prior to loading onto individual HG-U133_Plus_2 GeneChip®. The microarrays were hybridized at 45°C for 16- 24 hours and washed and stained on an Affymetrix FS450 fluidics station according to manufacturer recommendations.
| | Sample_scan_protocol | The microarrays were scanned on a GeneChip® Scanner 3000.
| | Sample_data_processing | All of the array files were normalized using the Robust Multi-Array Average (RMA) method
| | Sample_platform_id | GPL570
| | Sample_contact_name | Shelley ,,des Etages
| | Sample_contact_email | shelley.g.desetages@pfizer.com
| | Sample_contact_institute | Pfizer Inc.
| | Sample_contact_address | Eastern Point Road
| | Sample_contact_city | Groton
| | Sample_contact_state | CT
| | Sample_contact_zip/postal_code | 06340
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM601nnn/GSM601638/suppl/GSM601638_Exp_131_3434_h2_stromal_no_TNF_no_insulin_18.CEL.gz
| | Sample_series_id | GSE24422
| | Sample_data_row_count | 54675
| | |
|
GSM601639 | GPL570 |
|
Strom_Ins_rep 1
|
hMSC adipocytes, 5 hrs insulin, pelleted fraction
|
cell type: Stromal
treatment 2: 20 nM Insulin
|
|
| Sample_geo_accession | GSM601639
| | Sample_status | Public on Nov 30 2010
| | Sample_submission_date | Sep 28 2010
| | Sample_last_update_date | Sep 28 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | On Day 19 of differentiation, cells were treated with 0 or 10ng/ml TNF and incubated for 24hrs. Cells then underwent a 5 hr serum starvation followed by teatment with 0 or 20nM insulin for 2 hrs. At the end of the incubation, cells were harvested ( mixed) or fractioanted into adipocyte ( buiyant) and stromal (pelleted) fractions and subjected to RNA isolation.
| | Sample_growth_protocol_ch1 | Human mesenchymal stem cells were purchased from Lonza and cultured in basal media (Lonza PT3238) with mesenchymal stem cell growth supplement (Lonza PT4105). Cells were seeded at a density of 5000 cells/cm2. At confluence, the cells were differentiated into adipocytes using the adipogenic differentiation kit (Lonza PT3004). The total differentiation process took 19 days, with 14 days of induction and 5 days of maintenance. All media were from Lonza, except that the insulin concentration (in both induction and maintenance media) was adjusted to 120 pM. Mature adipocytes were subjected to 1% formaldehyde cross-linking for 10 minutes at room temperature and neutralized with 0.125M glycine for 5 minutes. Cells were then treated with Accutase and scraped. The preparation was centrifuged for 25 minutes at 15°C at 3500 rpm. The adipocytes, formed as a white layer (anti-pellet) at the meniscus, were harvested and snap-frozen in liquid nitrogen.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Frozen cells were resuspended in Qiagen RLT buffer and total RNA isolated using the Qiagen RNeasy Kit.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | cDNA was synthesized using 1-2 ug of high quality total RNA. Supercript II™ (Invitrogen) was used to reverse-transcribe the mRNA in the presence of a T7 containing oligo dT24 primer followed by second strand synthesis as recommended by Affymetrix. cDNA was purified using Agencourt RNAClean magnetic beads and used as a template for in vitro transcription using the GeneChip® One-Cycle Labeling Kit from Affymetrix. cRNA was purified using Agencourt magnetic beads and the quantity and purity of the cRNA was determined by absorbance at 260 nm and 260/280 absorbance ratio respectively. The quality of the cRNA was evaluated by assessing the size distribution of the cRNA using a 1.25% MOPS gel.
| | Sample_hyb_protocol | The labeled cRNA was fragmented as recommended by Affymetrix and 10 µg was added to a hybridization cocktail prior to loading onto individual HG-U133_Plus_2 GeneChip®. The microarrays were hybridized at 45°C for 16- 24 hours and washed and stained on an Affymetrix FS450 fluidics station according to manufacturer recommendations.
| | Sample_scan_protocol | The microarrays were scanned on a GeneChip® Scanner 3000.
| | Sample_data_processing | All of the array files were normalized using the Robust Multi-Array Average (RMA) method
| | Sample_platform_id | GPL570
| | Sample_contact_name | Shelley ,,des Etages
| | Sample_contact_email | shelley.g.desetages@pfizer.com
| | Sample_contact_institute | Pfizer Inc.
| | Sample_contact_address | Eastern Point Road
| | Sample_contact_city | Groton
| | Sample_contact_state | CT
| | Sample_contact_zip/postal_code | 06340
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM601nnn/GSM601639/suppl/GSM601639_Exp_131_3434_h2_stromal_no_TNF_20_nM_Insulin_19.CEL.gz
| | Sample_series_id | GSE24422
| | Sample_data_row_count | 54675
| | |
|
GSM601640 | GPL570 |
|
Strom_Ins_rep 2
|
hMSC adipocytes, 5 hrs insulin, pelleted fraction
|
cell type: Stromal
treatment 2: 20 nM Insulin
|
|
| Sample_geo_accession | GSM601640
| | Sample_status | Public on Nov 30 2010
| | Sample_submission_date | Sep 28 2010
| | Sample_last_update_date | Sep 28 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | On Day 19 of differentiation, cells were treated with 0 or 10ng/ml TNF and incubated for 24hrs. Cells then underwent a 5 hr serum starvation followed by teatment with 0 or 20nM insulin for 2 hrs. At the end of the incubation, cells were harvested ( mixed) or fractioanted into adipocyte ( buiyant) and stromal (pelleted) fractions and subjected to RNA isolation.
| | Sample_growth_protocol_ch1 | Human mesenchymal stem cells were purchased from Lonza and cultured in basal media (Lonza PT3238) with mesenchymal stem cell growth supplement (Lonza PT4105). Cells were seeded at a density of 5000 cells/cm2. At confluence, the cells were differentiated into adipocytes using the adipogenic differentiation kit (Lonza PT3004). The total differentiation process took 19 days, with 14 days of induction and 5 days of maintenance. All media were from Lonza, except that the insulin concentration (in both induction and maintenance media) was adjusted to 120 pM. Mature adipocytes were subjected to 1% formaldehyde cross-linking for 10 minutes at room temperature and neutralized with 0.125M glycine for 5 minutes. Cells were then treated with Accutase and scraped. The preparation was centrifuged for 25 minutes at 15°C at 3500 rpm. The adipocytes, formed as a white layer (anti-pellet) at the meniscus, were harvested and snap-frozen in liquid nitrogen.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Frozen cells were resuspended in Qiagen RLT buffer and total RNA isolated using the Qiagen RNeasy Kit.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | cDNA was synthesized using 1-2 ug of high quality total RNA. Supercript II™ (Invitrogen) was used to reverse-transcribe the mRNA in the presence of a T7 containing oligo dT24 primer followed by second strand synthesis as recommended by Affymetrix. cDNA was purified using Agencourt RNAClean magnetic beads and used as a template for in vitro transcription using the GeneChip® One-Cycle Labeling Kit from Affymetrix. cRNA was purified using Agencourt magnetic beads and the quantity and purity of the cRNA was determined by absorbance at 260 nm and 260/280 absorbance ratio respectively. The quality of the cRNA was evaluated by assessing the size distribution of the cRNA using a 1.25% MOPS gel.
| | Sample_hyb_protocol | The labeled cRNA was fragmented as recommended by Affymetrix and 10 µg was added to a hybridization cocktail prior to loading onto individual HG-U133_Plus_2 GeneChip®. The microarrays were hybridized at 45°C for 16- 24 hours and washed and stained on an Affymetrix FS450 fluidics station according to manufacturer recommendations.
| | Sample_scan_protocol | The microarrays were scanned on a GeneChip® Scanner 3000.
| | Sample_data_processing | All of the array files were normalized using the Robust Multi-Array Average (RMA) method
| | Sample_platform_id | GPL570
| | Sample_contact_name | Shelley ,,des Etages
| | Sample_contact_email | shelley.g.desetages@pfizer.com
| | Sample_contact_institute | Pfizer Inc.
| | Sample_contact_address | Eastern Point Road
| | Sample_contact_city | Groton
| | Sample_contact_state | CT
| | Sample_contact_zip/postal_code | 06340
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM601nnn/GSM601640/suppl/GSM601640_Exp_131_3434_h2_stromal_no_TNF_20_nM_Insulin_20.CEL.gz
| | Sample_series_id | GSE24422
| | Sample_data_row_count | 54675
| | |
|
GSM601641 | GPL570 |
|
Strom_TNF_rep 1
|
hMSC adipocytes, 24 hrs TNF, pelleted fraction
|
cell type: Stromal
treatment 1: 10 ng/ml TNF
|
|
| Sample_geo_accession | GSM601641
| | Sample_status | Public on Nov 30 2010
| | Sample_submission_date | Sep 28 2010
| | Sample_last_update_date | Sep 28 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | On Day 19 of differentiation, cells were treated with 0 or 10ng/ml TNF and incubated for 24hrs. Cells then underwent a 5 hr serum starvation followed by teatment with 0 or 20nM insulin for 2 hrs. At the end of the incubation, cells were harvested ( mixed) or fractioanted into adipocyte ( buiyant) and stromal (pelleted) fractions and subjected to RNA isolation.
| | Sample_growth_protocol_ch1 | Human mesenchymal stem cells were purchased from Lonza and cultured in basal media (Lonza PT3238) with mesenchymal stem cell growth supplement (Lonza PT4105). Cells were seeded at a density of 5000 cells/cm2. At confluence, the cells were differentiated into adipocytes using the adipogenic differentiation kit (Lonza PT3004). The total differentiation process took 19 days, with 14 days of induction and 5 days of maintenance. All media were from Lonza, except that the insulin concentration (in both induction and maintenance media) was adjusted to 120 pM. Mature adipocytes were subjected to 1% formaldehyde cross-linking for 10 minutes at room temperature and neutralized with 0.125M glycine for 5 minutes. Cells were then treated with Accutase and scraped. The preparation was centrifuged for 25 minutes at 15°C at 3500 rpm. The adipocytes, formed as a white layer (anti-pellet) at the meniscus, were harvested and snap-frozen in liquid nitrogen.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Frozen cells were resuspended in Qiagen RLT buffer and total RNA isolated using the Qiagen RNeasy Kit.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | cDNA was synthesized using 1-2 ug of high quality total RNA. Supercript II™ (Invitrogen) was used to reverse-transcribe the mRNA in the presence of a T7 containing oligo dT24 primer followed by second strand synthesis as recommended by Affymetrix. cDNA was purified using Agencourt RNAClean magnetic beads and used as a template for in vitro transcription using the GeneChip® One-Cycle Labeling Kit from Affymetrix. cRNA was purified using Agencourt magnetic beads and the quantity and purity of the cRNA was determined by absorbance at 260 nm and 260/280 absorbance ratio respectively. The quality of the cRNA was evaluated by assessing the size distribution of the cRNA using a 1.25% MOPS gel.
| | Sample_hyb_protocol | The labeled cRNA was fragmented as recommended by Affymetrix and 10 µg was added to a hybridization cocktail prior to loading onto individual HG-U133_Plus_2 GeneChip®. The microarrays were hybridized at 45°C for 16- 24 hours and washed and stained on an Affymetrix FS450 fluidics station according to manufacturer recommendations.
| | Sample_scan_protocol | The microarrays were scanned on a GeneChip® Scanner 3000.
| | Sample_data_processing | All of the array files were normalized using the Robust Multi-Array Average (RMA) method
| | Sample_platform_id | GPL570
| | Sample_contact_name | Shelley ,,des Etages
| | Sample_contact_email | shelley.g.desetages@pfizer.com
| | Sample_contact_institute | Pfizer Inc.
| | Sample_contact_address | Eastern Point Road
| | Sample_contact_city | Groton
| | Sample_contact_state | CT
| | Sample_contact_zip/postal_code | 06340
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM601nnn/GSM601641/suppl/GSM601641_Exp_131_3434_h2_stromal_TNF_no_insulin_21.CEL.gz
| | Sample_series_id | GSE24422
| | Sample_data_row_count | 54675
| | |
|
GSM601642 | GPL570 |
|
Strom_TNF_rep 2
|
hMSC adipocytes, 24 hrs TNF, pelleted fraction
|
cell type: Stromal
treatment 1: 10 ng/ml TNF
|
|
| Sample_geo_accession | GSM601642
| | Sample_status | Public on Nov 30 2010
| | Sample_submission_date | Sep 28 2010
| | Sample_last_update_date | Sep 28 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | On Day 19 of differentiation, cells were treated with 0 or 10ng/ml TNF and incubated for 24hrs. Cells then underwent a 5 hr serum starvation followed by teatment with 0 or 20nM insulin for 2 hrs. At the end of the incubation, cells were harvested ( mixed) or fractioanted into adipocyte ( buiyant) and stromal (pelleted) fractions and subjected to RNA isolation.
| | Sample_growth_protocol_ch1 | Human mesenchymal stem cells were purchased from Lonza and cultured in basal media (Lonza PT3238) with mesenchymal stem cell growth supplement (Lonza PT4105). Cells were seeded at a density of 5000 cells/cm2. At confluence, the cells were differentiated into adipocytes using the adipogenic differentiation kit (Lonza PT3004). The total differentiation process took 19 days, with 14 days of induction and 5 days of maintenance. All media were from Lonza, except that the insulin concentration (in both induction and maintenance media) was adjusted to 120 pM. Mature adipocytes were subjected to 1% formaldehyde cross-linking for 10 minutes at room temperature and neutralized with 0.125M glycine for 5 minutes. Cells were then treated with Accutase and scraped. The preparation was centrifuged for 25 minutes at 15°C at 3500 rpm. The adipocytes, formed as a white layer (anti-pellet) at the meniscus, were harvested and snap-frozen in liquid nitrogen.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Frozen cells were resuspended in Qiagen RLT buffer and total RNA isolated using the Qiagen RNeasy Kit.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | cDNA was synthesized using 1-2 ug of high quality total RNA. Supercript II™ (Invitrogen) was used to reverse-transcribe the mRNA in the presence of a T7 containing oligo dT24 primer followed by second strand synthesis as recommended by Affymetrix. cDNA was purified using Agencourt RNAClean magnetic beads and used as a template for in vitro transcription using the GeneChip® One-Cycle Labeling Kit from Affymetrix. cRNA was purified using Agencourt magnetic beads and the quantity and purity of the cRNA was determined by absorbance at 260 nm and 260/280 absorbance ratio respectively. The quality of the cRNA was evaluated by assessing the size distribution of the cRNA using a 1.25% MOPS gel.
| | Sample_hyb_protocol | The labeled cRNA was fragmented as recommended by Affymetrix and 10 µg was added to a hybridization cocktail prior to loading onto individual HG-U133_Plus_2 GeneChip®. The microarrays were hybridized at 45°C for 16- 24 hours and washed and stained on an Affymetrix FS450 fluidics station according to manufacturer recommendations.
| | Sample_scan_protocol | The microarrays were scanned on a GeneChip® Scanner 3000.
| | Sample_data_processing | All of the array files were normalized using the Robust Multi-Array Average (RMA) method
| | Sample_platform_id | GPL570
| | Sample_contact_name | Shelley ,,des Etages
| | Sample_contact_email | shelley.g.desetages@pfizer.com
| | Sample_contact_institute | Pfizer Inc.
| | Sample_contact_address | Eastern Point Road
| | Sample_contact_city | Groton
| | Sample_contact_state | CT
| | Sample_contact_zip/postal_code | 06340
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM601nnn/GSM601642/suppl/GSM601642_Exp_131_3434_h2_stromal_TNF_no_insulin_22.CEL.gz
| | Sample_series_id | GSE24422
| | Sample_data_row_count | 54675
| | |
|
GSM601643 | GPL570 |
|
Strom_TNF_Ins_rep 1
|
hMSC adipocytes, 24h TNF, 5 h insulin, pelleted fraction
|
cell type: Stromal
treatment 1: 10 ng/ml TNF
treatment 2: 20 nM Insulin
|
|
| Sample_geo_accession | GSM601643
| | Sample_status | Public on Nov 30 2010
| | Sample_submission_date | Sep 28 2010
| | Sample_last_update_date | Sep 28 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | On Day 19 of differentiation, cells were treated with 0 or 10ng/ml TNF and incubated for 24hrs. Cells then underwent a 5 hr serum starvation followed by teatment with 0 or 20nM insulin for 2 hrs. At the end of the incubation, cells were harvested ( mixed) or fractioanted into adipocyte ( buiyant) and stromal (pelleted) fractions and subjected to RNA isolation.
| | Sample_growth_protocol_ch1 | Human mesenchymal stem cells were purchased from Lonza and cultured in basal media (Lonza PT3238) with mesenchymal stem cell growth supplement (Lonza PT4105). Cells were seeded at a density of 5000 cells/cm2. At confluence, the cells were differentiated into adipocytes using the adipogenic differentiation kit (Lonza PT3004). The total differentiation process took 19 days, with 14 days of induction and 5 days of maintenance. All media were from Lonza, except that the insulin concentration (in both induction and maintenance media) was adjusted to 120 pM. Mature adipocytes were subjected to 1% formaldehyde cross-linking for 10 minutes at room temperature and neutralized with 0.125M glycine for 5 minutes. Cells were then treated with Accutase and scraped. The preparation was centrifuged for 25 minutes at 15°C at 3500 rpm. The adipocytes, formed as a white layer (anti-pellet) at the meniscus, were harvested and snap-frozen in liquid nitrogen.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Frozen cells were resuspended in Qiagen RLT buffer and total RNA isolated using the Qiagen RNeasy Kit.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | cDNA was synthesized using 1-2 ug of high quality total RNA. Supercript II™ (Invitrogen) was used to reverse-transcribe the mRNA in the presence of a T7 containing oligo dT24 primer followed by second strand synthesis as recommended by Affymetrix. cDNA was purified using Agencourt RNAClean magnetic beads and used as a template for in vitro transcription using the GeneChip® One-Cycle Labeling Kit from Affymetrix. cRNA was purified using Agencourt magnetic beads and the quantity and purity of the cRNA was determined by absorbance at 260 nm and 260/280 absorbance ratio respectively. The quality of the cRNA was evaluated by assessing the size distribution of the cRNA using a 1.25% MOPS gel.
| | Sample_hyb_protocol | The labeled cRNA was fragmented as recommended by Affymetrix and 10 µg was added to a hybridization cocktail prior to loading onto individual HG-U133_Plus_2 GeneChip®. The microarrays were hybridized at 45°C for 16- 24 hours and washed and stained on an Affymetrix FS450 fluidics station according to manufacturer recommendations.
| | Sample_scan_protocol | The microarrays were scanned on a GeneChip® Scanner 3000.
| | Sample_data_processing | All of the array files were normalized using the Robust Multi-Array Average (RMA) method
| | Sample_platform_id | GPL570
| | Sample_contact_name | Shelley ,,des Etages
| | Sample_contact_email | shelley.g.desetages@pfizer.com
| | Sample_contact_institute | Pfizer Inc.
| | Sample_contact_address | Eastern Point Road
| | Sample_contact_city | Groton
| | Sample_contact_state | CT
| | Sample_contact_zip/postal_code | 06340
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM601nnn/GSM601643/suppl/GSM601643_Exp_131_3434_h2_stromal_TNF_20nM_Insulin_23.CEL.gz
| | Sample_series_id | GSE24422
| | Sample_data_row_count | 54675
| | |
|
GSM601644 | GPL570 |
|
Strom_TNF_Ins_rep 2
|
hMSC adipocytes, 24h TNF, 5 h insulin, pelleted fraction
|
cell type: Stromal
treatment 1: 10 ng/ml TNF
treatment 2: 20 nM Insulin
|
|
| Sample_geo_accession | GSM601644
| | Sample_status | Public on Nov 30 2010
| | Sample_submission_date | Sep 28 2010
| | Sample_last_update_date | Sep 28 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | On Day 19 of differentiation, cells were treated with 0 or 10ng/ml TNF and incubated for 24hrs. Cells then underwent a 5 hr serum starvation followed by teatment with 0 or 20nM insulin for 2 hrs. At the end of the incubation, cells were harvested ( mixed) or fractioanted into adipocyte ( buiyant) and stromal (pelleted) fractions and subjected to RNA isolation.
| | Sample_growth_protocol_ch1 | Human mesenchymal stem cells were purchased from Lonza and cultured in basal media (Lonza PT3238) with mesenchymal stem cell growth supplement (Lonza PT4105). Cells were seeded at a density of 5000 cells/cm2. At confluence, the cells were differentiated into adipocytes using the adipogenic differentiation kit (Lonza PT3004). The total differentiation process took 19 days, with 14 days of induction and 5 days of maintenance. All media were from Lonza, except that the insulin concentration (in both induction and maintenance media) was adjusted to 120 pM. Mature adipocytes were subjected to 1% formaldehyde cross-linking for 10 minutes at room temperature and neutralized with 0.125M glycine for 5 minutes. Cells were then treated with Accutase and scraped. The preparation was centrifuged for 25 minutes at 15°C at 3500 rpm. The adipocytes, formed as a white layer (anti-pellet) at the meniscus, were harvested and snap-frozen in liquid nitrogen.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Frozen cells were resuspended in Qiagen RLT buffer and total RNA isolated using the Qiagen RNeasy Kit.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | cDNA was synthesized using 1-2 ug of high quality total RNA. Supercript II™ (Invitrogen) was used to reverse-transcribe the mRNA in the presence of a T7 containing oligo dT24 primer followed by second strand synthesis as recommended by Affymetrix. cDNA was purified using Agencourt RNAClean magnetic beads and used as a template for in vitro transcription using the GeneChip® One-Cycle Labeling Kit from Affymetrix. cRNA was purified using Agencourt magnetic beads and the quantity and purity of the cRNA was determined by absorbance at 260 nm and 260/280 absorbance ratio respectively. The quality of the cRNA was evaluated by assessing the size distribution of the cRNA using a 1.25% MOPS gel.
| | Sample_hyb_protocol | The labeled cRNA was fragmented as recommended by Affymetrix and 10 µg was added to a hybridization cocktail prior to loading onto individual HG-U133_Plus_2 GeneChip®. The microarrays were hybridized at 45°C for 16- 24 hours and washed and stained on an Affymetrix FS450 fluidics station according to manufacturer recommendations.
| | Sample_scan_protocol | The microarrays were scanned on a GeneChip® Scanner 3000.
| | Sample_data_processing | All of the array files were normalized using the Robust Multi-Array Average (RMA) method
| | Sample_platform_id | GPL570
| | Sample_contact_name | Shelley ,,des Etages
| | Sample_contact_email | shelley.g.desetages@pfizer.com
| | Sample_contact_institute | Pfizer Inc.
| | Sample_contact_address | Eastern Point Road
| | Sample_contact_city | Groton
| | Sample_contact_state | CT
| | Sample_contact_zip/postal_code | 06340
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM601nnn/GSM601644/suppl/GSM601644_Exp_131_3434_h2_stromal_TNF_20nM_Insulin_24.CEL.gz
| | Sample_series_id | GSE24422
| | Sample_data_row_count | 54675
| | |
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