Search results for the GEO ID: GSE24454 |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM602545 | GPL1261 |
|
JBM328
|
left ventricular myocardium
|
strain: C57BL/6
stress: banding and subsequent debanding (DB3)
|
3 days after debanding
|
Sample_geo_accession | GSM602545
| Sample_status | Public on Sep 30 2010
| Sample_submission_date | Sep 30 2010
| Sample_last_update_date | Sep 30 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | Following saccrifice, the left ventricle was separated from the right ventricle and the atria and snap frozen in liquid nitrogen and stored in a superfreezer untill processing.
| Sample_growth_protocol_ch1 | 7-week-old male C57BL/6 mice were sbjected to aortic banding (AB) or sham (sAB). After 4 weeks a debanding operation (DB/sDB) was performed. Mice were sacrificed 4 weeks after banding/sham or 3 days after debanding.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was isolated with an RNeasy Mini Kit from Qiagen according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 3 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45°C on GeneChip Mouse Genome 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner 3000.
| Sample_data_processing | Microarray pre-processing involved background adjustment using gcrma (Guanine Cytosine Robust Multi-Array Analysis) which normalizes data and adjusts for background intensities taking optical noise and non-specific binding into account and control of false discovery rate (FDR) according to Benjamini and Hochberg.
| Sample_platform_id | GPL1261
| Sample_contact_name | Johannes,L,Bjørnstad
| Sample_contact_email | j.l.bjornstad@medisin.uio.no
| Sample_contact_department | Dept. of Cardiothoracic Surgery
| Sample_contact_institute | Oslo University Hospital Ulleval
| Sample_contact_address | Kirkeveien 166
| Sample_contact_city | Oslo
| Sample_contact_zip/postal_code | NO-0424
| Sample_contact_country | Norway
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM602nnn/GSM602545/suppl/GSM602545.CEL.gz
| Sample_series_id | GSE24454
| Sample_data_row_count | 45101
| |
|
GSM602546 | GPL1261 |
|
JBM382
|
left ventricular myocardium
|
strain: C57BL/6
stress: sham procedure and subsequent debanding (sDB3)
|
3 days after debanding (sham)
|
Sample_geo_accession | GSM602546
| Sample_status | Public on Sep 30 2010
| Sample_submission_date | Sep 30 2010
| Sample_last_update_date | Sep 30 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | Following saccrifice, the left ventricle was separated from the right ventricle and the atria and snap frozen in liquid nitrogen and stored in a superfreezer untill processing.
| Sample_growth_protocol_ch1 | 7-week-old male C57BL/6 mice were sbjected to aortic banding (AB) or sham (sAB). After 4 weeks a debanding operation (DB/sDB) was performed. Mice were sacrificed 4 weeks after banding/sham or 3 days after debanding.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was isolated with an RNeasy Mini Kit from Qiagen according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 3 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45°C on GeneChip Mouse Genome 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner 3000.
| Sample_data_processing | Microarray pre-processing involved background adjustment using gcrma (Guanine Cytosine Robust Multi-Array Analysis) which normalizes data and adjusts for background intensities taking optical noise and non-specific binding into account and control of false discovery rate (FDR) according to Benjamini and Hochberg.
| Sample_platform_id | GPL1261
| Sample_contact_name | Johannes,L,Bjørnstad
| Sample_contact_email | j.l.bjornstad@medisin.uio.no
| Sample_contact_department | Dept. of Cardiothoracic Surgery
| Sample_contact_institute | Oslo University Hospital Ulleval
| Sample_contact_address | Kirkeveien 166
| Sample_contact_city | Oslo
| Sample_contact_zip/postal_code | NO-0424
| Sample_contact_country | Norway
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM602nnn/GSM602546/suppl/GSM602546.CEL.gz
| Sample_series_id | GSE24454
| Sample_data_row_count | 45101
| |
|
GSM602547 | GPL1261 |
|
JBM401
|
left ventricular myocardium
|
strain: C57BL/6
stress: sham procedure and subsequent debanding (sDB3)
|
3 days after debanding (sham)
|
Sample_geo_accession | GSM602547
| Sample_status | Public on Sep 30 2010
| Sample_submission_date | Sep 30 2010
| Sample_last_update_date | Sep 30 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | Following saccrifice, the left ventricle was separated from the right ventricle and the atria and snap frozen in liquid nitrogen and stored in a superfreezer untill processing.
| Sample_growth_protocol_ch1 | 7-week-old male C57BL/6 mice were sbjected to aortic banding (AB) or sham (sAB). After 4 weeks a debanding operation (DB/sDB) was performed. Mice were sacrificed 4 weeks after banding/sham or 3 days after debanding.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was isolated with an RNeasy Mini Kit from Qiagen according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 3 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45°C on GeneChip Mouse Genome 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner 3000.
| Sample_data_processing | Microarray pre-processing involved background adjustment using gcrma (Guanine Cytosine Robust Multi-Array Analysis) which normalizes data and adjusts for background intensities taking optical noise and non-specific binding into account and control of false discovery rate (FDR) according to Benjamini and Hochberg.
| Sample_platform_id | GPL1261
| Sample_contact_name | Johannes,L,Bjørnstad
| Sample_contact_email | j.l.bjornstad@medisin.uio.no
| Sample_contact_department | Dept. of Cardiothoracic Surgery
| Sample_contact_institute | Oslo University Hospital Ulleval
| Sample_contact_address | Kirkeveien 166
| Sample_contact_city | Oslo
| Sample_contact_zip/postal_code | NO-0424
| Sample_contact_country | Norway
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM602nnn/GSM602547/suppl/GSM602547.CEL.gz
| Sample_series_id | GSE24454
| Sample_data_row_count | 45101
| |
|
GSM602548 | GPL1261 |
|
JBM431
|
left ventricular myocardium
|
strain: C57BL/6
stress: banding and subsequent debanding (DB3)
|
3 days after debanding
|
Sample_geo_accession | GSM602548
| Sample_status | Public on Sep 30 2010
| Sample_submission_date | Sep 30 2010
| Sample_last_update_date | Sep 30 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | Following saccrifice, the left ventricle was separated from the right ventricle and the atria and snap frozen in liquid nitrogen and stored in a superfreezer untill processing.
| Sample_growth_protocol_ch1 | 7-week-old male C57BL/6 mice were sbjected to aortic banding (AB) or sham (sAB). After 4 weeks a debanding operation (DB/sDB) was performed. Mice were sacrificed 4 weeks after banding/sham or 3 days after debanding.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was isolated with an RNeasy Mini Kit from Qiagen according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 3 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45°C on GeneChip Mouse Genome 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner 3000.
| Sample_data_processing | Microarray pre-processing involved background adjustment using gcrma (Guanine Cytosine Robust Multi-Array Analysis) which normalizes data and adjusts for background intensities taking optical noise and non-specific binding into account and control of false discovery rate (FDR) according to Benjamini and Hochberg.
| Sample_platform_id | GPL1261
| Sample_contact_name | Johannes,L,Bjørnstad
| Sample_contact_email | j.l.bjornstad@medisin.uio.no
| Sample_contact_department | Dept. of Cardiothoracic Surgery
| Sample_contact_institute | Oslo University Hospital Ulleval
| Sample_contact_address | Kirkeveien 166
| Sample_contact_city | Oslo
| Sample_contact_zip/postal_code | NO-0424
| Sample_contact_country | Norway
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM602nnn/GSM602548/suppl/GSM602548.CEL.gz
| Sample_series_id | GSE24454
| Sample_data_row_count | 45101
| |
|
GSM602549 | GPL1261 |
|
JBM432
|
left ventricular myocardium
|
strain: C57BL/6
stress: banding and subsequent debanding (DB3)
|
3 days after debanding
|
Sample_geo_accession | GSM602549
| Sample_status | Public on Sep 30 2010
| Sample_submission_date | Sep 30 2010
| Sample_last_update_date | Sep 30 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | Following saccrifice, the left ventricle was separated from the right ventricle and the atria and snap frozen in liquid nitrogen and stored in a superfreezer untill processing.
| Sample_growth_protocol_ch1 | 7-week-old male C57BL/6 mice were sbjected to aortic banding (AB) or sham (sAB). After 4 weeks a debanding operation (DB/sDB) was performed. Mice were sacrificed 4 weeks after banding/sham or 3 days after debanding.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was isolated with an RNeasy Mini Kit from Qiagen according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 3 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45°C on GeneChip Mouse Genome 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner 3000.
| Sample_data_processing | Microarray pre-processing involved background adjustment using gcrma (Guanine Cytosine Robust Multi-Array Analysis) which normalizes data and adjusts for background intensities taking optical noise and non-specific binding into account and control of false discovery rate (FDR) according to Benjamini and Hochberg.
| Sample_platform_id | GPL1261
| Sample_contact_name | Johannes,L,Bjørnstad
| Sample_contact_email | j.l.bjornstad@medisin.uio.no
| Sample_contact_department | Dept. of Cardiothoracic Surgery
| Sample_contact_institute | Oslo University Hospital Ulleval
| Sample_contact_address | Kirkeveien 166
| Sample_contact_city | Oslo
| Sample_contact_zip/postal_code | NO-0424
| Sample_contact_country | Norway
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM602nnn/GSM602549/suppl/GSM602549.CEL.gz
| Sample_series_id | GSE24454
| Sample_data_row_count | 45101
| |
|
GSM602550 | GPL1261 |
|
JBM438
|
left ventricular myocardium
|
strain: C57BL/6
stress: sham procedure and subsequent debanding (sDB3)
|
3 days after debanding (sham)
|
Sample_geo_accession | GSM602550
| Sample_status | Public on Sep 30 2010
| Sample_submission_date | Sep 30 2010
| Sample_last_update_date | Sep 30 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | Following saccrifice, the left ventricle was separated from the right ventricle and the atria and snap frozen in liquid nitrogen and stored in a superfreezer untill processing.
| Sample_growth_protocol_ch1 | 7-week-old male C57BL/6 mice were sbjected to aortic banding (AB) or sham (sAB). After 4 weeks a debanding operation (DB/sDB) was performed. Mice were sacrificed 4 weeks after banding/sham or 3 days after debanding.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was isolated with an RNeasy Mini Kit from Qiagen according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 3 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45°C on GeneChip Mouse Genome 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner 3000.
| Sample_data_processing | Microarray pre-processing involved background adjustment using gcrma (Guanine Cytosine Robust Multi-Array Analysis) which normalizes data and adjusts for background intensities taking optical noise and non-specific binding into account and control of false discovery rate (FDR) according to Benjamini and Hochberg.
| Sample_platform_id | GPL1261
| Sample_contact_name | Johannes,L,Bjørnstad
| Sample_contact_email | j.l.bjornstad@medisin.uio.no
| Sample_contact_department | Dept. of Cardiothoracic Surgery
| Sample_contact_institute | Oslo University Hospital Ulleval
| Sample_contact_address | Kirkeveien 166
| Sample_contact_city | Oslo
| Sample_contact_zip/postal_code | NO-0424
| Sample_contact_country | Norway
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM602nnn/GSM602550/suppl/GSM602550.CEL.gz
| Sample_series_id | GSE24454
| Sample_data_row_count | 45101
| |
|
GSM602551 | GPL1261 |
|
JBM439
|
left ventricular myocardium
|
strain: C57BL/6
stress: aortic banding (AB)
|
4 weeks after aortic banding
|
Sample_geo_accession | GSM602551
| Sample_status | Public on Sep 30 2010
| Sample_submission_date | Sep 30 2010
| Sample_last_update_date | Sep 30 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | Following saccrifice, the left ventricle was separated from the right ventricle and the atria and snap frozen in liquid nitrogen and stored in a superfreezer untill processing.
| Sample_growth_protocol_ch1 | 7-week-old male C57BL/6 mice were sbjected to aortic banding (AB) or sham (sAB). After 4 weeks a debanding operation (DB/sDB) was performed. Mice were sacrificed 4 weeks after banding/sham or 3 days after debanding.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was isolated with an RNeasy Mini Kit from Qiagen according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 3 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45°C on GeneChip Mouse Genome 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner 3000.
| Sample_data_processing | Microarray pre-processing involved background adjustment using gcrma (Guanine Cytosine Robust Multi-Array Analysis) which normalizes data and adjusts for background intensities taking optical noise and non-specific binding into account and control of false discovery rate (FDR) according to Benjamini and Hochberg.
| Sample_platform_id | GPL1261
| Sample_contact_name | Johannes,L,Bjørnstad
| Sample_contact_email | j.l.bjornstad@medisin.uio.no
| Sample_contact_department | Dept. of Cardiothoracic Surgery
| Sample_contact_institute | Oslo University Hospital Ulleval
| Sample_contact_address | Kirkeveien 166
| Sample_contact_city | Oslo
| Sample_contact_zip/postal_code | NO-0424
| Sample_contact_country | Norway
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM602nnn/GSM602551/suppl/GSM602551.CEL.gz
| Sample_series_id | GSE24454
| Sample_data_row_count | 45101
| |
|
GSM602552 | GPL1261 |
|
JBM442
|
left ventricular myocardium
|
strain: C57BL/6
stress: aortic banding (AB)
|
4 weeks after aortic banding
|
Sample_geo_accession | GSM602552
| Sample_status | Public on Sep 30 2010
| Sample_submission_date | Sep 30 2010
| Sample_last_update_date | Sep 30 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | Following saccrifice, the left ventricle was separated from the right ventricle and the atria and snap frozen in liquid nitrogen and stored in a superfreezer untill processing.
| Sample_growth_protocol_ch1 | 7-week-old male C57BL/6 mice were sbjected to aortic banding (AB) or sham (sAB). After 4 weeks a debanding operation (DB/sDB) was performed. Mice were sacrificed 4 weeks after banding/sham or 3 days after debanding.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was isolated with an RNeasy Mini Kit from Qiagen according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 3 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45°C on GeneChip Mouse Genome 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner 3000.
| Sample_data_processing | Microarray pre-processing involved background adjustment using gcrma (Guanine Cytosine Robust Multi-Array Analysis) which normalizes data and adjusts for background intensities taking optical noise and non-specific binding into account and control of false discovery rate (FDR) according to Benjamini and Hochberg.
| Sample_platform_id | GPL1261
| Sample_contact_name | Johannes,L,Bjørnstad
| Sample_contact_email | j.l.bjornstad@medisin.uio.no
| Sample_contact_department | Dept. of Cardiothoracic Surgery
| Sample_contact_institute | Oslo University Hospital Ulleval
| Sample_contact_address | Kirkeveien 166
| Sample_contact_city | Oslo
| Sample_contact_zip/postal_code | NO-0424
| Sample_contact_country | Norway
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM602nnn/GSM602552/suppl/GSM602552.CEL.gz
| Sample_series_id | GSE24454
| Sample_data_row_count | 45101
| |
|
GSM602553 | GPL1261 |
|
JBM445
|
left ventricular myocardium
|
strain: C57BL/6
stress: aortic banding (AB)
|
4 weeks after aortic banding
|
Sample_geo_accession | GSM602553
| Sample_status | Public on Sep 30 2010
| Sample_submission_date | Sep 30 2010
| Sample_last_update_date | Sep 30 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | Following saccrifice, the left ventricle was separated from the right ventricle and the atria and snap frozen in liquid nitrogen and stored in a superfreezer untill processing.
| Sample_growth_protocol_ch1 | 7-week-old male C57BL/6 mice were sbjected to aortic banding (AB) or sham (sAB). After 4 weeks a debanding operation (DB/sDB) was performed. Mice were sacrificed 4 weeks after banding/sham or 3 days after debanding.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was isolated with an RNeasy Mini Kit from Qiagen according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 3 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45°C on GeneChip Mouse Genome 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner 3000.
| Sample_data_processing | Microarray pre-processing involved background adjustment using gcrma (Guanine Cytosine Robust Multi-Array Analysis) which normalizes data and adjusts for background intensities taking optical noise and non-specific binding into account and control of false discovery rate (FDR) according to Benjamini and Hochberg.
| Sample_platform_id | GPL1261
| Sample_contact_name | Johannes,L,Bjørnstad
| Sample_contact_email | j.l.bjornstad@medisin.uio.no
| Sample_contact_department | Dept. of Cardiothoracic Surgery
| Sample_contact_institute | Oslo University Hospital Ulleval
| Sample_contact_address | Kirkeveien 166
| Sample_contact_city | Oslo
| Sample_contact_zip/postal_code | NO-0424
| Sample_contact_country | Norway
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM602nnn/GSM602553/suppl/GSM602553.CEL.gz
| Sample_series_id | GSE24454
| Sample_data_row_count | 45101
| |
|
GSM602554 | GPL1261 |
|
JBM493
|
left ventricular myocardium
|
strain: C57BL/6
stress: sham procedure (sAB)
|
4 weeks after aortic banding (sham)
|
Sample_geo_accession | GSM602554
| Sample_status | Public on Sep 30 2010
| Sample_submission_date | Sep 30 2010
| Sample_last_update_date | Sep 30 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | Following saccrifice, the left ventricle was separated from the right ventricle and the atria and snap frozen in liquid nitrogen and stored in a superfreezer untill processing.
| Sample_growth_protocol_ch1 | 7-week-old male C57BL/6 mice were sbjected to aortic banding (AB) or sham (sAB). After 4 weeks a debanding operation (DB/sDB) was performed. Mice were sacrificed 4 weeks after banding/sham or 3 days after debanding.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was isolated with an RNeasy Mini Kit from Qiagen according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 3 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45°C on GeneChip Mouse Genome 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner 3000.
| Sample_data_processing | Microarray pre-processing involved background adjustment using gcrma (Guanine Cytosine Robust Multi-Array Analysis) which normalizes data and adjusts for background intensities taking optical noise and non-specific binding into account and control of false discovery rate (FDR) according to Benjamini and Hochberg.
| Sample_platform_id | GPL1261
| Sample_contact_name | Johannes,L,Bjørnstad
| Sample_contact_email | j.l.bjornstad@medisin.uio.no
| Sample_contact_department | Dept. of Cardiothoracic Surgery
| Sample_contact_institute | Oslo University Hospital Ulleval
| Sample_contact_address | Kirkeveien 166
| Sample_contact_city | Oslo
| Sample_contact_zip/postal_code | NO-0424
| Sample_contact_country | Norway
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM602nnn/GSM602554/suppl/GSM602554.CEL.gz
| Sample_series_id | GSE24454
| Sample_data_row_count | 45101
| |
|
GSM602555 | GPL1261 |
|
JBM499
|
left ventricular myocardium
|
strain: C57BL/6
stress: sham procedure (sAB)
|
4 weeks after aortic banding (sham)
|
Sample_geo_accession | GSM602555
| Sample_status | Public on Sep 30 2010
| Sample_submission_date | Sep 30 2010
| Sample_last_update_date | Sep 30 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | Following saccrifice, the left ventricle was separated from the right ventricle and the atria and snap frozen in liquid nitrogen and stored in a superfreezer untill processing.
| Sample_growth_protocol_ch1 | 7-week-old male C57BL/6 mice were sbjected to aortic banding (AB) or sham (sAB). After 4 weeks a debanding operation (DB/sDB) was performed. Mice were sacrificed 4 weeks after banding/sham or 3 days after debanding.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was isolated with an RNeasy Mini Kit from Qiagen according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 3 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45°C on GeneChip Mouse Genome 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner 3000.
| Sample_data_processing | Microarray pre-processing involved background adjustment using gcrma (Guanine Cytosine Robust Multi-Array Analysis) which normalizes data and adjusts for background intensities taking optical noise and non-specific binding into account and control of false discovery rate (FDR) according to Benjamini and Hochberg.
| Sample_platform_id | GPL1261
| Sample_contact_name | Johannes,L,Bjørnstad
| Sample_contact_email | j.l.bjornstad@medisin.uio.no
| Sample_contact_department | Dept. of Cardiothoracic Surgery
| Sample_contact_institute | Oslo University Hospital Ulleval
| Sample_contact_address | Kirkeveien 166
| Sample_contact_city | Oslo
| Sample_contact_zip/postal_code | NO-0424
| Sample_contact_country | Norway
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM602nnn/GSM602555/suppl/GSM602555.CEL.gz
| Sample_series_id | GSE24454
| Sample_data_row_count | 45101
| |
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GSM602556 | GPL1261 |
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JBM504
|
left ventricular myocardium
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strain: C57BL/6
stress: sham procedure (sAB)
|
4 weeks after aortic banding (sham)
|
Sample_geo_accession | GSM602556
| Sample_status | Public on Sep 30 2010
| Sample_submission_date | Sep 30 2010
| Sample_last_update_date | Sep 30 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | Following saccrifice, the left ventricle was separated from the right ventricle and the atria and snap frozen in liquid nitrogen and stored in a superfreezer untill processing.
| Sample_growth_protocol_ch1 | 7-week-old male C57BL/6 mice were sbjected to aortic banding (AB) or sham (sAB). After 4 weeks a debanding operation (DB/sDB) was performed. Mice were sacrificed 4 weeks after banding/sham or 3 days after debanding.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was isolated with an RNeasy Mini Kit from Qiagen according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 3 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45°C on GeneChip Mouse Genome 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner 3000.
| Sample_data_processing | Microarray pre-processing involved background adjustment using gcrma (Guanine Cytosine Robust Multi-Array Analysis) which normalizes data and adjusts for background intensities taking optical noise and non-specific binding into account and control of false discovery rate (FDR) according to Benjamini and Hochberg.
| Sample_platform_id | GPL1261
| Sample_contact_name | Johannes,L,Bjørnstad
| Sample_contact_email | j.l.bjornstad@medisin.uio.no
| Sample_contact_department | Dept. of Cardiothoracic Surgery
| Sample_contact_institute | Oslo University Hospital Ulleval
| Sample_contact_address | Kirkeveien 166
| Sample_contact_city | Oslo
| Sample_contact_zip/postal_code | NO-0424
| Sample_contact_country | Norway
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM602nnn/GSM602556/suppl/GSM602556.CEL.gz
| Sample_series_id | GSE24454
| Sample_data_row_count | 45101
| |
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