Result

Search results for the GEO ID: GSE24461

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GSM ID

GPL ID

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Title

Source name

Description

Characteristics

GSM602662

GPL1261

Liver sample of p14 KO mouse 1

Six week old male C57BL/6 mouse with conditional liver p14 knock out.

tissue: liver genotype: p14 KO genetic background: C57BL/6

Expression profile of liver sample from p14 KO mouse, biological replicate 1.

Sample_geo_accession	GSM602662
Sample_status	Public on Feb 01 2011
Sample_submission_date	Sep 30 2010
Sample_last_update_date	Feb 01 2011
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_growth_protocol_ch1	The animals were raised and cared for in accordance with the guidelines established by the Innsbruck Medical University, Austria
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Liver samples from 6 week old p14 KO and control mice (gender matched) were homogenized in 1ml of Trizol Reagent using a tissue hand-homogenizer (5 ml, Unilab Politakis OHG). The extraction of total RNA was performed according to the manufacturer's directions, and the RNA was subsequently purified by passage through Rneasy mini-columns (QIAGEN, Valencia, CA).
Sample_label_ch1	biotin
Sample_label_protocol_ch1	1ug total RNA was processed to generate a biotinylated hybridization target using “One Cycle cDNA Synthesis” and “One Cycle Target Labelling” kits from Affymetrix (Affymetrix, Santa Clara, CA). All procedures were performed according to the manufacturer’s protocols.
Sample_hyb_protocol	20ug of cRNA were fragmented at 95C using the Affymetrix fragmentation buffer, mixed with hybridization buffer containing hybridization controls and hybridized to Affymetrix Mouse Genome 430 2.0 GeneChips.
Sample_scan_protocol	Fluorescence signals were recorded by an Affymetrix scanner 3000 and image analysis performed with the GCOS software.
Sample_data_processing	The raw signal intensities were proprocessed in R (version 2.9.1) using Bioconductor's gcrma package (version 2.16.0).
Sample_platform_id	GPL1261
Sample_contact_name	Johannes,,Rainer
Sample_contact_email	johannes.rainer@i-med.ac.at
Sample_contact_laboratory	Applied Bioinformatics Group
Sample_contact_department	Biocenter, Division Molecular Pathophysiology
Sample_contact_institute	Medical University Innsbruck
Sample_contact_address	Innrain 80-82 II
Sample_contact_city	Innsbruck
Sample_contact_zip/postal_code	6020
Sample_contact_country	Austria
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM602nnn/GSM602662/suppl/GSM602662_1009_023_m37_m01_MB_250708.CEL.gz
Sample_series_id	GSE24461
Sample_data_row_count	45101

GSM602663

GPL1261

Liver sample of p14 KO mouse 2

Six week old male C57BL/6 mouse with conditional liver p14 knock out.

tissue: liver genotype: p14 KO genetic background: C57BL/6

Expression profile of liver sample from p14 KO mouse, biological replicate 2.

Sample_geo_accession	GSM602663
Sample_status	Public on Feb 01 2011
Sample_submission_date	Sep 30 2010
Sample_last_update_date	Feb 01 2011
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_growth_protocol_ch1	The animals were raised and cared for in accordance with the guidelines established by the Innsbruck Medical University, Austria
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Liver samples from 6 week old p14 KO and control mice (gender matched) were homogenized in 1ml of Trizol Reagent using a tissue hand-homogenizer (5 ml, Unilab Politakis OHG). The extraction of total RNA was performed according to the manufacturer's directions, and the RNA was subsequently purified by passage through Rneasy mini-columns (QIAGEN, Valencia, CA).
Sample_label_ch1	biotin
Sample_label_protocol_ch1	1ug total RNA was processed to generate a biotinylated hybridization target using “One Cycle cDNA Synthesis” and “One Cycle Target Labelling” kits from Affymetrix (Affymetrix, Santa Clara, CA). All procedures were performed according to the manufacturer’s protocols.
Sample_hyb_protocol	20ug of cRNA were fragmented at 95C using the Affymetrix fragmentation buffer, mixed with hybridization buffer containing hybridization controls and hybridized to Affymetrix Mouse Genome 430 2.0 GeneChips.
Sample_scan_protocol	Fluorescence signals were recorded by an Affymetrix scanner 3000 and image analysis performed with the GCOS software.
Sample_data_processing	The raw signal intensities were proprocessed in R (version 2.9.1) using Bioconductor's gcrma package (version 2.16.0).
Sample_platform_id	GPL1261
Sample_contact_name	Johannes,,Rainer
Sample_contact_email	johannes.rainer@i-med.ac.at
Sample_contact_laboratory	Applied Bioinformatics Group
Sample_contact_department	Biocenter, Division Molecular Pathophysiology
Sample_contact_institute	Medical University Innsbruck
Sample_contact_address	Innrain 80-82 II
Sample_contact_city	Innsbruck
Sample_contact_zip/postal_code	6020
Sample_contact_country	Austria
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM602nnn/GSM602663/suppl/GSM602663_1010_023_m39_m01_MB_250708.CEL.gz
Sample_series_id	GSE24461
Sample_data_row_count	45101

GSM602664

GPL1261

Liver sample of p14 KO mouse 3

Six week old male C57BL/6 mouse with conditional liver p14 knock out.

tissue: liver genotype: p14 KO genetic background: C57BL/6

Expression profile of liver sample from p14 KO mouse, biological replicate 3.

Sample_geo_accession	GSM602664
Sample_status	Public on Feb 01 2011
Sample_submission_date	Sep 30 2010
Sample_last_update_date	Feb 01 2011
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_growth_protocol_ch1	The animals were raised and cared for in accordance with the guidelines established by the Innsbruck Medical University, Austria
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Liver samples from 6 week old p14 KO and control mice (gender matched) were homogenized in 1ml of Trizol Reagent using a tissue hand-homogenizer (5 ml, Unilab Politakis OHG). The extraction of total RNA was performed according to the manufacturer's directions, and the RNA was subsequently purified by passage through Rneasy mini-columns (QIAGEN, Valencia, CA).
Sample_label_ch1	biotin
Sample_label_protocol_ch1	1ug total RNA was processed to generate a biotinylated hybridization target using “One Cycle cDNA Synthesis” and “One Cycle Target Labelling” kits from Affymetrix (Affymetrix, Santa Clara, CA). All procedures were performed according to the manufacturer’s protocols.
Sample_hyb_protocol	20ug of cRNA were fragmented at 95C using the Affymetrix fragmentation buffer, mixed with hybridization buffer containing hybridization controls and hybridized to Affymetrix Mouse Genome 430 2.0 GeneChips.
Sample_scan_protocol	Fluorescence signals were recorded by an Affymetrix scanner 3000 and image analysis performed with the GCOS software.
Sample_data_processing	The raw signal intensities were proprocessed in R (version 2.9.1) using Bioconductor's gcrma package (version 2.16.0).
Sample_platform_id	GPL1261
Sample_contact_name	Johannes,,Rainer
Sample_contact_email	johannes.rainer@i-med.ac.at
Sample_contact_laboratory	Applied Bioinformatics Group
Sample_contact_department	Biocenter, Division Molecular Pathophysiology
Sample_contact_institute	Medical University Innsbruck
Sample_contact_address	Innrain 80-82 II
Sample_contact_city	Innsbruck
Sample_contact_zip/postal_code	6020
Sample_contact_country	Austria
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM602nnn/GSM602664/suppl/GSM602664_1013_023_m61_m01_MB_250708.CEL.gz
Sample_series_id	GSE24461
Sample_data_row_count	45101

GSM602665

GPL1261

Liver sample of WT mouse 1

Six week old male C57BL/6 mouse.

tissue: liver genotype: p14 WT genetic background: C57BL/6

Expression profile of liver sample from control mouse, biological replicate 1.

Sample_geo_accession	GSM602665
Sample_status	Public on Feb 01 2011
Sample_submission_date	Sep 30 2010
Sample_last_update_date	Feb 01 2011
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_growth_protocol_ch1	The animals were raised and cared for in accordance with the guidelines established by the Innsbruck Medical University, Austria
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Liver samples from 6 week old p14 KO and control mice (gender matched) were homogenized in 1ml of Trizol Reagent using a tissue hand-homogenizer (5 ml, Unilab Politakis OHG). The extraction of total RNA was performed according to the manufacturer's directions, and the RNA was subsequently purified by passage through Rneasy mini-columns (QIAGEN, Valencia, CA).
Sample_label_ch1	biotin
Sample_label_protocol_ch1	1ug total RNA was processed to generate a biotinylated hybridization target using “One Cycle cDNA Synthesis” and “One Cycle Target Labelling” kits from Affymetrix (Affymetrix, Santa Clara, CA). All procedures were performed according to the manufacturer’s protocols.
Sample_hyb_protocol	20ug of cRNA were fragmented at 95C using the Affymetrix fragmentation buffer, mixed with hybridization buffer containing hybridization controls and hybridized to Affymetrix Mouse Genome 430 2.0 GeneChips.
Sample_scan_protocol	Fluorescence signals were recorded by an Affymetrix scanner 3000 and image analysis performed with the GCOS software.
Sample_data_processing	The raw signal intensities were proprocessed in R (version 2.9.1) using Bioconductor's gcrma package (version 2.16.0).
Sample_platform_id	GPL1261
Sample_contact_name	Johannes,,Rainer
Sample_contact_email	johannes.rainer@i-med.ac.at
Sample_contact_laboratory	Applied Bioinformatics Group
Sample_contact_department	Biocenter, Division Molecular Pathophysiology
Sample_contact_institute	Medical University Innsbruck
Sample_contact_address	Innrain 80-82 II
Sample_contact_city	Innsbruck
Sample_contact_zip/postal_code	6020
Sample_contact_country	Austria
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM602nnn/GSM602665/suppl/GSM602665_1011_023_m70_m01_MB_250708.CEL.gz
Sample_series_id	GSE24461
Sample_data_row_count	45101

GSM602666

GPL1261

Liver sample of WT mouse 2

Six week old male C57BL/6 mouse.

tissue: liver genotype: p14 WT genetic background: C57BL/6

Expression profile of liver sample from control mouse, biological replicate 2.

Sample_geo_accession	GSM602666
Sample_status	Public on Feb 01 2011
Sample_submission_date	Sep 30 2010
Sample_last_update_date	Feb 01 2011
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_growth_protocol_ch1	The animals were raised and cared for in accordance with the guidelines established by the Innsbruck Medical University, Austria
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Liver samples from 6 week old p14 KO and control mice (gender matched) were homogenized in 1ml of Trizol Reagent using a tissue hand-homogenizer (5 ml, Unilab Politakis OHG). The extraction of total RNA was performed according to the manufacturer's directions, and the RNA was subsequently purified by passage through Rneasy mini-columns (QIAGEN, Valencia, CA).
Sample_label_ch1	biotin
Sample_label_protocol_ch1	1ug total RNA was processed to generate a biotinylated hybridization target using “One Cycle cDNA Synthesis” and “One Cycle Target Labelling” kits from Affymetrix (Affymetrix, Santa Clara, CA). All procedures were performed according to the manufacturer’s protocols.
Sample_hyb_protocol	20ug of cRNA were fragmented at 95C using the Affymetrix fragmentation buffer, mixed with hybridization buffer containing hybridization controls and hybridized to Affymetrix Mouse Genome 430 2.0 GeneChips.
Sample_scan_protocol	Fluorescence signals were recorded by an Affymetrix scanner 3000 and image analysis performed with the GCOS software.
Sample_data_processing	The raw signal intensities were proprocessed in R (version 2.9.1) using Bioconductor's gcrma package (version 2.16.0).
Sample_platform_id	GPL1261
Sample_contact_name	Johannes,,Rainer
Sample_contact_email	johannes.rainer@i-med.ac.at
Sample_contact_laboratory	Applied Bioinformatics Group
Sample_contact_department	Biocenter, Division Molecular Pathophysiology
Sample_contact_institute	Medical University Innsbruck
Sample_contact_address	Innrain 80-82 II
Sample_contact_city	Innsbruck
Sample_contact_zip/postal_code	6020
Sample_contact_country	Austria
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM602nnn/GSM602666/suppl/GSM602666_1012_023_m72_m01_MB_250708.CEL.gz
Sample_series_id	GSE24461
Sample_data_row_count	45101

GSM602667

GPL1261

Liver sample of WT mouse 3

Six week old male C57BL/6 mouse.

tissue: liver genotype: p14 WT genetic background: C57BL/6

Expression profile of liver sample from control mouse, biological replicate 3.

Sample_geo_accession	GSM602667
Sample_status	Public on Feb 01 2011
Sample_submission_date	Sep 30 2010
Sample_last_update_date	Feb 01 2011
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_growth_protocol_ch1	The animals were raised and cared for in accordance with the guidelines established by the Innsbruck Medical University, Austria
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Liver samples from 6 week old p14 KO and control mice (gender matched) were homogenized in 1ml of Trizol Reagent using a tissue hand-homogenizer (5 ml, Unilab Politakis OHG). The extraction of total RNA was performed according to the manufacturer's directions, and the RNA was subsequently purified by passage through Rneasy mini-columns (QIAGEN, Valencia, CA).
Sample_label_ch1	biotin
Sample_label_protocol_ch1	1ug total RNA was processed to generate a biotinylated hybridization target using “One Cycle cDNA Synthesis” and “One Cycle Target Labelling” kits from Affymetrix (Affymetrix, Santa Clara, CA). All procedures were performed according to the manufacturer’s protocols.
Sample_hyb_protocol	20ug of cRNA were fragmented at 95C using the Affymetrix fragmentation buffer, mixed with hybridization buffer containing hybridization controls and hybridized to Affymetrix Mouse Genome 430 2.0 GeneChips.
Sample_scan_protocol	Fluorescence signals were recorded by an Affymetrix scanner 3000 and image analysis performed with the GCOS software.
Sample_data_processing	The raw signal intensities were proprocessed in R (version 2.9.1) using Bioconductor's gcrma package (version 2.16.0).
Sample_platform_id	GPL1261
Sample_contact_name	Johannes,,Rainer
Sample_contact_email	johannes.rainer@i-med.ac.at
Sample_contact_laboratory	Applied Bioinformatics Group
Sample_contact_department	Biocenter, Division Molecular Pathophysiology
Sample_contact_institute	Medical University Innsbruck
Sample_contact_address	Innrain 80-82 II
Sample_contact_city	Innsbruck
Sample_contact_zip/postal_code	6020
Sample_contact_country	Austria
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM602nnn/GSM602667/suppl/GSM602667_1014_023_m73_m01_MB_250708.CEL.gz
Sample_series_id	GSE24461
Sample_data_row_count	45101

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