Search results for the GEO ID: GSE24468  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM602697 | GPL570 |
|
R5020 Repl. 1
|
hMEC
|
ligand: R5020 10 nM
cell type: mammary epithelial cell
|
|
| Sample_geo_accession | GSM602697
| | Sample_status | Public on Oct 01 2010
| | Sample_submission_date | Sep 30 2010
| | Sample_last_update_date | Sep 30 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Adenoviruses overexpressing hPR-B or β-Gal were generated using the ViraPower Adenoviral Expression System (Invitrogen), and were amplified and purified by CsCl2 centrifugation. Cells were infected at a multiplicity of infection (MOI) of 150 for 17.5 hr. Following infection, cells were treated with 10nM R5020 or vehicle for 16 hours.
| | Sample_growth_protocol_ch1 | Human mammary epithelial cells were were maintained in MEBM (Lonza, Basel, Switzerland) supplemented with MEGM SingleQuots (Lonza) at 37 degrees C in 5% CO2 atmosphere
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated by using either RNeasy kit (Qiagen, Valencia, CA) or Aurum total RNA mini kit (Bio-Rad, Hercules, CA).
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Affymetrix 1-cycle IVT
| | Sample_hyb_protocol | standard Affymetrix protocol
| | Sample_scan_protocol | Affymetrix GeneChip scanner; Pixel size 1.56, Filter 570.
| | Sample_data_processing | Raw data were processed using JMP Genomics 4.1 (SAS). Background subtraction: RMA; Normalization: quantile; Transformation: Log2; Summarization: median polish.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Dmitri,A,Kazmin
| | Sample_contact_email | kazmi002@mc.duke.edu
| | Sample_contact_phone | 919-681-1348
| | Sample_contact_fax | 919-681-7139
| | Sample_contact_laboratory | McDonnell
| | Sample_contact_department | Pharmacology and Cancer Biology
| | Sample_contact_institute | Duke University Medical Center
| | Sample_contact_address | C264 LSRC, Research Dr
| | Sample_contact_city | Durham
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 27710
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM602nnn/GSM602697/suppl/GSM602697_1121_35232_H133+_12662_B1_+_.CEL.gz
| | Sample_series_id | GSE24468
| | Sample_data_row_count | 54613
| | |
|
GSM602698 | GPL570 |
|
R5020 Repl. 2
|
hMEC
|
ligand: R5020 10 nM
cell type: mammary epithelial cell
|
|
| Sample_geo_accession | GSM602698
| | Sample_status | Public on Oct 01 2010
| | Sample_submission_date | Sep 30 2010
| | Sample_last_update_date | Sep 30 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Adenoviruses overexpressing hPR-B or β-Gal were generated using the ViraPower Adenoviral Expression System (Invitrogen), and were amplified and purified by CsCl2 centrifugation. Cells were infected at a multiplicity of infection (MOI) of 150 for 17.5 hr. Following infection, cells were treated with 10nM R5020 or vehicle for 16 hours.
| | Sample_growth_protocol_ch1 | Human mammary epithelial cells were were maintained in MEBM (Lonza, Basel, Switzerland) supplemented with MEGM SingleQuots (Lonza) at 37 degrees C in 5% CO2 atmosphere
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated by using either RNeasy kit (Qiagen, Valencia, CA) or Aurum total RNA mini kit (Bio-Rad, Hercules, CA).
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Affymetrix 1-cycle IVT
| | Sample_hyb_protocol | standard Affymetrix protocol
| | Sample_scan_protocol | Affymetrix GeneChip scanner; Pixel size 1.56, Filter 570.
| | Sample_data_processing | Raw data were processed using JMP Genomics 4.1 (SAS). Background subtraction: RMA; Normalization: quantile; Transformation: Log2; Summarization: median polish.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Dmitri,A,Kazmin
| | Sample_contact_email | kazmi002@mc.duke.edu
| | Sample_contact_phone | 919-681-1348
| | Sample_contact_fax | 919-681-7139
| | Sample_contact_laboratory | McDonnell
| | Sample_contact_department | Pharmacology and Cancer Biology
| | Sample_contact_institute | Duke University Medical Center
| | Sample_contact_address | C264 LSRC, Research Dr
| | Sample_contact_city | Durham
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 27710
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM602nnn/GSM602698/suppl/GSM602698_1121_35233_H133+_12663_B2_+_.CEL.gz
| | Sample_series_id | GSE24468
| | Sample_data_row_count | 54613
| | |
|
GSM602699 | GPL570 |
|
R5020 Repl. 3
|
hMEC
|
ligand: R5020 10 nM
cell type: mammary epithelial cell
|
|
| Sample_geo_accession | GSM602699
| | Sample_status | Public on Oct 01 2010
| | Sample_submission_date | Sep 30 2010
| | Sample_last_update_date | Sep 30 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Adenoviruses overexpressing hPR-B or β-Gal were generated using the ViraPower Adenoviral Expression System (Invitrogen), and were amplified and purified by CsCl2 centrifugation. Cells were infected at a multiplicity of infection (MOI) of 150 for 17.5 hr. Following infection, cells were treated with 10nM R5020 or vehicle for 16 hours.
| | Sample_growth_protocol_ch1 | Human mammary epithelial cells were were maintained in MEBM (Lonza, Basel, Switzerland) supplemented with MEGM SingleQuots (Lonza) at 37 degrees C in 5% CO2 atmosphere
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated by using either RNeasy kit (Qiagen, Valencia, CA) or Aurum total RNA mini kit (Bio-Rad, Hercules, CA).
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Affymetrix 1-cycle IVT
| | Sample_hyb_protocol | standard Affymetrix protocol
| | Sample_scan_protocol | Affymetrix GeneChip scanner; Pixel size 1.56, Filter 570.
| | Sample_data_processing | Raw data were processed using JMP Genomics 4.1 (SAS). Background subtraction: RMA; Normalization: quantile; Transformation: Log2; Summarization: median polish.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Dmitri,A,Kazmin
| | Sample_contact_email | kazmi002@mc.duke.edu
| | Sample_contact_phone | 919-681-1348
| | Sample_contact_fax | 919-681-7139
| | Sample_contact_laboratory | McDonnell
| | Sample_contact_department | Pharmacology and Cancer Biology
| | Sample_contact_institute | Duke University Medical Center
| | Sample_contact_address | C264 LSRC, Research Dr
| | Sample_contact_city | Durham
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 27710
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM602nnn/GSM602699/suppl/GSM602699_1121_35234_H133+_12664_B3_+_.CEL.gz
| | Sample_series_id | GSE24468
| | Sample_data_row_count | 54613
| | |
|
GSM602700 | GPL570 |
|
R5020 Repl. 4
|
hMEC
|
ligand: R5020 10 nM
cell type: mammary epithelial cell
|
|
| Sample_geo_accession | GSM602700
| | Sample_status | Public on Oct 01 2010
| | Sample_submission_date | Sep 30 2010
| | Sample_last_update_date | Sep 30 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Adenoviruses overexpressing hPR-B or β-Gal were generated using the ViraPower Adenoviral Expression System (Invitrogen), and were amplified and purified by CsCl2 centrifugation. Cells were infected at a multiplicity of infection (MOI) of 150 for 17.5 hr. Following infection, cells were treated with 10nM R5020 or vehicle for 16 hours.
| | Sample_growth_protocol_ch1 | Human mammary epithelial cells were were maintained in MEBM (Lonza, Basel, Switzerland) supplemented with MEGM SingleQuots (Lonza) at 37 degrees C in 5% CO2 atmosphere
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated by using either RNeasy kit (Qiagen, Valencia, CA) or Aurum total RNA mini kit (Bio-Rad, Hercules, CA).
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Affymetrix 1-cycle IVT
| | Sample_hyb_protocol | standard Affymetrix protocol
| | Sample_scan_protocol | Affymetrix GeneChip scanner; Pixel size 1.56, Filter 570.
| | Sample_data_processing | Raw data were processed using JMP Genomics 4.1 (SAS). Background subtraction: RMA; Normalization: quantile; Transformation: Log2; Summarization: median polish.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Dmitri,A,Kazmin
| | Sample_contact_email | kazmi002@mc.duke.edu
| | Sample_contact_phone | 919-681-1348
| | Sample_contact_fax | 919-681-7139
| | Sample_contact_laboratory | McDonnell
| | Sample_contact_department | Pharmacology and Cancer Biology
| | Sample_contact_institute | Duke University Medical Center
| | Sample_contact_address | C264 LSRC, Research Dr
| | Sample_contact_city | Durham
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 27710
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM602nnn/GSM602700/suppl/GSM602700_1121_35235_H133+_12665_B4_+_@N2.CEL.gz
| | Sample_series_id | GSE24468
| | Sample_data_row_count | 54613
| | |
|
GSM602701 | GPL570 |
|
R5020 Repl. 5
|
hMEC
|
ligand: R5020 10 nM
cell type: mammary epithelial cell
|
|
| Sample_geo_accession | GSM602701
| | Sample_status | Public on Oct 01 2010
| | Sample_submission_date | Sep 30 2010
| | Sample_last_update_date | Sep 30 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Adenoviruses overexpressing hPR-B or β-Gal were generated using the ViraPower Adenoviral Expression System (Invitrogen), and were amplified and purified by CsCl2 centrifugation. Cells were infected at a multiplicity of infection (MOI) of 150 for 17.5 hr. Following infection, cells were treated with 10nM R5020 or vehicle for 16 hours.
| | Sample_growth_protocol_ch1 | Human mammary epithelial cells were were maintained in MEBM (Lonza, Basel, Switzerland) supplemented with MEGM SingleQuots (Lonza) at 37 degrees C in 5% CO2 atmosphere
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated by using either RNeasy kit (Qiagen, Valencia, CA) or Aurum total RNA mini kit (Bio-Rad, Hercules, CA).
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Affymetrix 1-cycle IVT
| | Sample_hyb_protocol | standard Affymetrix protocol
| | Sample_scan_protocol | Affymetrix GeneChip scanner; Pixel size 1.56, Filter 570.
| | Sample_data_processing | Raw data were processed using JMP Genomics 4.1 (SAS). Background subtraction: RMA; Normalization: quantile; Transformation: Log2; Summarization: median polish.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Dmitri,A,Kazmin
| | Sample_contact_email | kazmi002@mc.duke.edu
| | Sample_contact_phone | 919-681-1348
| | Sample_contact_fax | 919-681-7139
| | Sample_contact_laboratory | McDonnell
| | Sample_contact_department | Pharmacology and Cancer Biology
| | Sample_contact_institute | Duke University Medical Center
| | Sample_contact_address | C264 LSRC, Research Dr
| | Sample_contact_city | Durham
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 27710
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM602nnn/GSM602701/suppl/GSM602701_1121_35236_H133+_12666_B5_+_.CEL.gz
| | Sample_series_id | GSE24468
| | Sample_data_row_count | 54613
| | |
|
GSM602702 | GPL570 |
|
R5020 Repl. 6
|
hMEC
|
ligand: R5020 10 nM
cell type: mammary epithelial cell
|
|
| Sample_geo_accession | GSM602702
| | Sample_status | Public on Oct 01 2010
| | Sample_submission_date | Sep 30 2010
| | Sample_last_update_date | Sep 30 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Adenoviruses overexpressing hPR-B or β-Gal were generated using the ViraPower Adenoviral Expression System (Invitrogen), and were amplified and purified by CsCl2 centrifugation. Cells were infected at a multiplicity of infection (MOI) of 150 for 17.5 hr. Following infection, cells were treated with 10nM R5020 or vehicle for 16 hours.
| | Sample_growth_protocol_ch1 | Human mammary epithelial cells were were maintained in MEBM (Lonza, Basel, Switzerland) supplemented with MEGM SingleQuots (Lonza) at 37 degrees C in 5% CO2 atmosphere
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated by using either RNeasy kit (Qiagen, Valencia, CA) or Aurum total RNA mini kit (Bio-Rad, Hercules, CA).
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Affymetrix 1-cycle IVT
| | Sample_hyb_protocol | standard Affymetrix protocol
| | Sample_scan_protocol | Affymetrix GeneChip scanner; Pixel size 1.56, Filter 570.
| | Sample_data_processing | Raw data were processed using JMP Genomics 4.1 (SAS). Background subtraction: RMA; Normalization: quantile; Transformation: Log2; Summarization: median polish.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Dmitri,A,Kazmin
| | Sample_contact_email | kazmi002@mc.duke.edu
| | Sample_contact_phone | 919-681-1348
| | Sample_contact_fax | 919-681-7139
| | Sample_contact_laboratory | McDonnell
| | Sample_contact_department | Pharmacology and Cancer Biology
| | Sample_contact_institute | Duke University Medical Center
| | Sample_contact_address | C264 LSRC, Research Dr
| | Sample_contact_city | Durham
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 27710
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM602nnn/GSM602702/suppl/GSM602702_1121_35237_H133+_12667_B6_+_.CEL.gz
| | Sample_series_id | GSE24468
| | Sample_data_row_count | 54613
| | |
|
GSM602703 | GPL570 |
|
R5020 Repl. 7
|
hMEC
|
ligand: R5020 10 nM
cell type: mammary epithelial cell
|
|
| Sample_geo_accession | GSM602703
| | Sample_status | Public on Oct 01 2010
| | Sample_submission_date | Sep 30 2010
| | Sample_last_update_date | Sep 30 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Adenoviruses overexpressing hPR-B or β-Gal were generated using the ViraPower Adenoviral Expression System (Invitrogen), and were amplified and purified by CsCl2 centrifugation. Cells were infected at a multiplicity of infection (MOI) of 150 for 17.5 hr. Following infection, cells were treated with 10nM R5020 or vehicle for 16 hours.
| | Sample_growth_protocol_ch1 | Human mammary epithelial cells were were maintained in MEBM (Lonza, Basel, Switzerland) supplemented with MEGM SingleQuots (Lonza) at 37 degrees C in 5% CO2 atmosphere
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated by using either RNeasy kit (Qiagen, Valencia, CA) or Aurum total RNA mini kit (Bio-Rad, Hercules, CA).
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Affymetrix 1-cycle IVT
| | Sample_hyb_protocol | standard Affymetrix protocol
| | Sample_scan_protocol | Affymetrix GeneChip scanner; Pixel size 1.56, Filter 570.
| | Sample_data_processing | Raw data were processed using JMP Genomics 4.1 (SAS). Background subtraction: RMA; Normalization: quantile; Transformation: Log2; Summarization: median polish.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Dmitri,A,Kazmin
| | Sample_contact_email | kazmi002@mc.duke.edu
| | Sample_contact_phone | 919-681-1348
| | Sample_contact_fax | 919-681-7139
| | Sample_contact_laboratory | McDonnell
| | Sample_contact_department | Pharmacology and Cancer Biology
| | Sample_contact_institute | Duke University Medical Center
| | Sample_contact_address | C264 LSRC, Research Dr
| | Sample_contact_city | Durham
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 27710
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM602nnn/GSM602703/suppl/GSM602703_1121_35238_H133+_12668_B7_+_.CEL.gz
| | Sample_series_id | GSE24468
| | Sample_data_row_count | 54613
| | |
|
GSM602704 | GPL570 |
|
R5020 Repl. 8
|
hMEC
|
ligand: R5020 10 nM
cell type: mammary epithelial cell
|
|
| Sample_geo_accession | GSM602704
| | Sample_status | Public on Oct 01 2010
| | Sample_submission_date | Sep 30 2010
| | Sample_last_update_date | Sep 30 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Adenoviruses overexpressing hPR-B or β-Gal were generated using the ViraPower Adenoviral Expression System (Invitrogen), and were amplified and purified by CsCl2 centrifugation. Cells were infected at a multiplicity of infection (MOI) of 150 for 17.5 hr. Following infection, cells were treated with 10nM R5020 or vehicle for 16 hours.
| | Sample_growth_protocol_ch1 | Human mammary epithelial cells were were maintained in MEBM (Lonza, Basel, Switzerland) supplemented with MEGM SingleQuots (Lonza) at 37 degrees C in 5% CO2 atmosphere
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated by using either RNeasy kit (Qiagen, Valencia, CA) or Aurum total RNA mini kit (Bio-Rad, Hercules, CA).
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Affymetrix 1-cycle IVT
| | Sample_hyb_protocol | standard Affymetrix protocol
| | Sample_scan_protocol | Affymetrix GeneChip scanner; Pixel size 1.56, Filter 570.
| | Sample_data_processing | Raw data were processed using JMP Genomics 4.1 (SAS). Background subtraction: RMA; Normalization: quantile; Transformation: Log2; Summarization: median polish.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Dmitri,A,Kazmin
| | Sample_contact_email | kazmi002@mc.duke.edu
| | Sample_contact_phone | 919-681-1348
| | Sample_contact_fax | 919-681-7139
| | Sample_contact_laboratory | McDonnell
| | Sample_contact_department | Pharmacology and Cancer Biology
| | Sample_contact_institute | Duke University Medical Center
| | Sample_contact_address | C264 LSRC, Research Dr
| | Sample_contact_city | Durham
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 27710
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM602nnn/GSM602704/suppl/GSM602704_1121_35239_H133+_12669_B8_+_.CEL.gz
| | Sample_series_id | GSE24468
| | Sample_data_row_count | 54613
| | |
|
GSM602705 | GPL570 |
|
R5020 Repl. 9
|
hMEC
|
ligand: R5020 10 nM
cell type: mammary epithelial cell
|
|
| Sample_geo_accession | GSM602705
| | Sample_status | Public on Oct 01 2010
| | Sample_submission_date | Sep 30 2010
| | Sample_last_update_date | Sep 30 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Adenoviruses overexpressing hPR-B or β-Gal were generated using the ViraPower Adenoviral Expression System (Invitrogen), and were amplified and purified by CsCl2 centrifugation. Cells were infected at a multiplicity of infection (MOI) of 150 for 17.5 hr. Following infection, cells were treated with 10nM R5020 or vehicle for 16 hours.
| | Sample_growth_protocol_ch1 | Human mammary epithelial cells were were maintained in MEBM (Lonza, Basel, Switzerland) supplemented with MEGM SingleQuots (Lonza) at 37 degrees C in 5% CO2 atmosphere
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated by using either RNeasy kit (Qiagen, Valencia, CA) or Aurum total RNA mini kit (Bio-Rad, Hercules, CA).
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Affymetrix 1-cycle IVT
| | Sample_hyb_protocol | standard Affymetrix protocol
| | Sample_scan_protocol | Affymetrix GeneChip scanner; Pixel size 1.56, Filter 570.
| | Sample_data_processing | Raw data were processed using JMP Genomics 4.1 (SAS). Background subtraction: RMA; Normalization: quantile; Transformation: Log2; Summarization: median polish.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Dmitri,A,Kazmin
| | Sample_contact_email | kazmi002@mc.duke.edu
| | Sample_contact_phone | 919-681-1348
| | Sample_contact_fax | 919-681-7139
| | Sample_contact_laboratory | McDonnell
| | Sample_contact_department | Pharmacology and Cancer Biology
| | Sample_contact_institute | Duke University Medical Center
| | Sample_contact_address | C264 LSRC, Research Dr
| | Sample_contact_city | Durham
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 27710
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM602nnn/GSM602705/suppl/GSM602705_1121_35240_H133+_12670_B9_+_@F2.CEL.gz
| | Sample_series_id | GSE24468
| | Sample_data_row_count | 54613
| | |
|
GSM602706 | GPL570 |
|
R5020 Repl. 10
|
hMEC
|
ligand: R5020 10 nM
cell type: mammary epithelial cell
|
|
| Sample_geo_accession | GSM602706
| | Sample_status | Public on Oct 01 2010
| | Sample_submission_date | Sep 30 2010
| | Sample_last_update_date | Sep 30 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Adenoviruses overexpressing hPR-B or β-Gal were generated using the ViraPower Adenoviral Expression System (Invitrogen), and were amplified and purified by CsCl2 centrifugation. Cells were infected at a multiplicity of infection (MOI) of 150 for 17.5 hr. Following infection, cells were treated with 10nM R5020 or vehicle for 16 hours.
| | Sample_growth_protocol_ch1 | Human mammary epithelial cells were were maintained in MEBM (Lonza, Basel, Switzerland) supplemented with MEGM SingleQuots (Lonza) at 37 degrees C in 5% CO2 atmosphere
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated by using either RNeasy kit (Qiagen, Valencia, CA) or Aurum total RNA mini kit (Bio-Rad, Hercules, CA).
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Affymetrix 1-cycle IVT
| | Sample_hyb_protocol | standard Affymetrix protocol
| | Sample_scan_protocol | Affymetrix GeneChip scanner; Pixel size 1.56, Filter 570.
| | Sample_data_processing | Raw data were processed using JMP Genomics 4.1 (SAS). Background subtraction: RMA; Normalization: quantile; Transformation: Log2; Summarization: median polish.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Dmitri,A,Kazmin
| | Sample_contact_email | kazmi002@mc.duke.edu
| | Sample_contact_phone | 919-681-1348
| | Sample_contact_fax | 919-681-7139
| | Sample_contact_laboratory | McDonnell
| | Sample_contact_department | Pharmacology and Cancer Biology
| | Sample_contact_institute | Duke University Medical Center
| | Sample_contact_address | C264 LSRC, Research Dr
| | Sample_contact_city | Durham
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 27710
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM602nnn/GSM602706/suppl/GSM602706_1121_35241_H133+_12671_B10_+_.CEL.gz
| | Sample_series_id | GSE24468
| | Sample_data_row_count | 54613
| | |
|
GSM602707 | GPL570 |
|
Vehicle Repl. 1
|
hMEC
|
ligand: Ethanol
cell type: mammary epithelial cell
|
|
| Sample_geo_accession | GSM602707
| | Sample_status | Public on Oct 01 2010
| | Sample_submission_date | Sep 30 2010
| | Sample_last_update_date | Sep 30 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Adenoviruses overexpressing hPR-B or β-Gal were generated using the ViraPower Adenoviral Expression System (Invitrogen), and were amplified and purified by CsCl2 centrifugation. Cells were infected at a multiplicity of infection (MOI) of 150 for 17.5 hr. Following infection, cells were treated with 10nM R5020 or vehicle for 16 hours.
| | Sample_growth_protocol_ch1 | Human mammary epithelial cells were were maintained in MEBM (Lonza, Basel, Switzerland) supplemented with MEGM SingleQuots (Lonza) at 37 degrees C in 5% CO2 atmosphere
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated by using either RNeasy kit (Qiagen, Valencia, CA) or Aurum total RNA mini kit (Bio-Rad, Hercules, CA).
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Affymetrix 1-cycle IVT
| | Sample_hyb_protocol | standard Affymetrix protocol
| | Sample_scan_protocol | Affymetrix GeneChip scanner; Pixel size 1.56, Filter 570.
| | Sample_data_processing | Raw data were processed using JMP Genomics 4.1 (SAS). Background subtraction: RMA; Normalization: quantile; Transformation: Log2; Summarization: median polish.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Dmitri,A,Kazmin
| | Sample_contact_email | kazmi002@mc.duke.edu
| | Sample_contact_phone | 919-681-1348
| | Sample_contact_fax | 919-681-7139
| | Sample_contact_laboratory | McDonnell
| | Sample_contact_department | Pharmacology and Cancer Biology
| | Sample_contact_institute | Duke University Medical Center
| | Sample_contact_address | C264 LSRC, Research Dr
| | Sample_contact_city | Durham
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 27710
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM602nnn/GSM602707/suppl/GSM602707_1121_35242_H133+_12672_B1_-_@F2.CEL.gz
| | Sample_series_id | GSE24468
| | Sample_data_row_count | 54613
| | |
|
GSM602708 | GPL570 |
|
Vehicle Repl. 2
|
hMEC
|
ligand: Ethanol
cell type: mammary epithelial cell
|
|
| Sample_geo_accession | GSM602708
| | Sample_status | Public on Oct 01 2010
| | Sample_submission_date | Sep 30 2010
| | Sample_last_update_date | Sep 30 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Adenoviruses overexpressing hPR-B or β-Gal were generated using the ViraPower Adenoviral Expression System (Invitrogen), and were amplified and purified by CsCl2 centrifugation. Cells were infected at a multiplicity of infection (MOI) of 150 for 17.5 hr. Following infection, cells were treated with 10nM R5020 or vehicle for 16 hours.
| | Sample_growth_protocol_ch1 | Human mammary epithelial cells were were maintained in MEBM (Lonza, Basel, Switzerland) supplemented with MEGM SingleQuots (Lonza) at 37 degrees C in 5% CO2 atmosphere
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated by using either RNeasy kit (Qiagen, Valencia, CA) or Aurum total RNA mini kit (Bio-Rad, Hercules, CA).
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Affymetrix 1-cycle IVT
| | Sample_hyb_protocol | standard Affymetrix protocol
| | Sample_scan_protocol | Affymetrix GeneChip scanner; Pixel size 1.56, Filter 570.
| | Sample_data_processing | Raw data were processed using JMP Genomics 4.1 (SAS). Background subtraction: RMA; Normalization: quantile; Transformation: Log2; Summarization: median polish.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Dmitri,A,Kazmin
| | Sample_contact_email | kazmi002@mc.duke.edu
| | Sample_contact_phone | 919-681-1348
| | Sample_contact_fax | 919-681-7139
| | Sample_contact_laboratory | McDonnell
| | Sample_contact_department | Pharmacology and Cancer Biology
| | Sample_contact_institute | Duke University Medical Center
| | Sample_contact_address | C264 LSRC, Research Dr
| | Sample_contact_city | Durham
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 27710
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM602nnn/GSM602708/suppl/GSM602708_1121_35243_H133+_12673_B2_-_.CEL.gz
| | Sample_series_id | GSE24468
| | Sample_data_row_count | 54613
| | |
|
GSM602709 | GPL570 |
|
Vehicle Repl. 3
|
hMEC
|
ligand: Ethanol
cell type: mammary epithelial cell
|
|
| Sample_geo_accession | GSM602709
| | Sample_status | Public on Oct 01 2010
| | Sample_submission_date | Sep 30 2010
| | Sample_last_update_date | Sep 30 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Adenoviruses overexpressing hPR-B or β-Gal were generated using the ViraPower Adenoviral Expression System (Invitrogen), and were amplified and purified by CsCl2 centrifugation. Cells were infected at a multiplicity of infection (MOI) of 150 for 17.5 hr. Following infection, cells were treated with 10nM R5020 or vehicle for 16 hours.
| | Sample_growth_protocol_ch1 | Human mammary epithelial cells were were maintained in MEBM (Lonza, Basel, Switzerland) supplemented with MEGM SingleQuots (Lonza) at 37 degrees C in 5% CO2 atmosphere
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated by using either RNeasy kit (Qiagen, Valencia, CA) or Aurum total RNA mini kit (Bio-Rad, Hercules, CA).
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Affymetrix 1-cycle IVT
| | Sample_hyb_protocol | standard Affymetrix protocol
| | Sample_scan_protocol | Affymetrix GeneChip scanner; Pixel size 1.56, Filter 570.
| | Sample_data_processing | Raw data were processed using JMP Genomics 4.1 (SAS). Background subtraction: RMA; Normalization: quantile; Transformation: Log2; Summarization: median polish.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Dmitri,A,Kazmin
| | Sample_contact_email | kazmi002@mc.duke.edu
| | Sample_contact_phone | 919-681-1348
| | Sample_contact_fax | 919-681-7139
| | Sample_contact_laboratory | McDonnell
| | Sample_contact_department | Pharmacology and Cancer Biology
| | Sample_contact_institute | Duke University Medical Center
| | Sample_contact_address | C264 LSRC, Research Dr
| | Sample_contact_city | Durham
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 27710
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM602nnn/GSM602709/suppl/GSM602709_1121_35244_H133+_12674_B3_-_.CEL.gz
| | Sample_series_id | GSE24468
| | Sample_data_row_count | 54613
| | |
|
GSM602710 | GPL570 |
|
Vehicle Repl. 4
|
hMEC
|
ligand: Ethanol
cell type: mammary epithelial cell
|
|
| Sample_geo_accession | GSM602710
| | Sample_status | Public on Oct 01 2010
| | Sample_submission_date | Sep 30 2010
| | Sample_last_update_date | Sep 30 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Adenoviruses overexpressing hPR-B or β-Gal were generated using the ViraPower Adenoviral Expression System (Invitrogen), and were amplified and purified by CsCl2 centrifugation. Cells were infected at a multiplicity of infection (MOI) of 150 for 17.5 hr. Following infection, cells were treated with 10nM R5020 or vehicle for 16 hours.
| | Sample_growth_protocol_ch1 | Human mammary epithelial cells were were maintained in MEBM (Lonza, Basel, Switzerland) supplemented with MEGM SingleQuots (Lonza) at 37 degrees C in 5% CO2 atmosphere
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated by using either RNeasy kit (Qiagen, Valencia, CA) or Aurum total RNA mini kit (Bio-Rad, Hercules, CA).
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Affymetrix 1-cycle IVT
| | Sample_hyb_protocol | standard Affymetrix protocol
| | Sample_scan_protocol | Affymetrix GeneChip scanner; Pixel size 1.56, Filter 570.
| | Sample_data_processing | Raw data were processed using JMP Genomics 4.1 (SAS). Background subtraction: RMA; Normalization: quantile; Transformation: Log2; Summarization: median polish.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Dmitri,A,Kazmin
| | Sample_contact_email | kazmi002@mc.duke.edu
| | Sample_contact_phone | 919-681-1348
| | Sample_contact_fax | 919-681-7139
| | Sample_contact_laboratory | McDonnell
| | Sample_contact_department | Pharmacology and Cancer Biology
| | Sample_contact_institute | Duke University Medical Center
| | Sample_contact_address | C264 LSRC, Research Dr
| | Sample_contact_city | Durham
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 27710
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM602nnn/GSM602710/suppl/GSM602710_1121_35245_H133+_12675_B4_-_.CEL.gz
| | Sample_series_id | GSE24468
| | Sample_data_row_count | 54613
| | |
|
GSM602711 | GPL570 |
|
Vehicle Repl. 5
|
hMEC
|
ligand: Ethanol
cell type: mammary epithelial cell
|
|
| Sample_geo_accession | GSM602711
| | Sample_status | Public on Oct 01 2010
| | Sample_submission_date | Sep 30 2010
| | Sample_last_update_date | Sep 30 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Adenoviruses overexpressing hPR-B or β-Gal were generated using the ViraPower Adenoviral Expression System (Invitrogen), and were amplified and purified by CsCl2 centrifugation. Cells were infected at a multiplicity of infection (MOI) of 150 for 17.5 hr. Following infection, cells were treated with 10nM R5020 or vehicle for 16 hours.
| | Sample_growth_protocol_ch1 | Human mammary epithelial cells were were maintained in MEBM (Lonza, Basel, Switzerland) supplemented with MEGM SingleQuots (Lonza) at 37 degrees C in 5% CO2 atmosphere
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated by using either RNeasy kit (Qiagen, Valencia, CA) or Aurum total RNA mini kit (Bio-Rad, Hercules, CA).
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Affymetrix 1-cycle IVT
| | Sample_hyb_protocol | standard Affymetrix protocol
| | Sample_scan_protocol | Affymetrix GeneChip scanner; Pixel size 1.56, Filter 570.
| | Sample_data_processing | Raw data were processed using JMP Genomics 4.1 (SAS). Background subtraction: RMA; Normalization: quantile; Transformation: Log2; Summarization: median polish.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Dmitri,A,Kazmin
| | Sample_contact_email | kazmi002@mc.duke.edu
| | Sample_contact_phone | 919-681-1348
| | Sample_contact_fax | 919-681-7139
| | Sample_contact_laboratory | McDonnell
| | Sample_contact_department | Pharmacology and Cancer Biology
| | Sample_contact_institute | Duke University Medical Center
| | Sample_contact_address | C264 LSRC, Research Dr
| | Sample_contact_city | Durham
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 27710
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM602nnn/GSM602711/suppl/GSM602711_1121_35246_H133+_12676_B5_-_.CEL.gz
| | Sample_series_id | GSE24468
| | Sample_data_row_count | 54613
| | |
|
GSM602712 | GPL570 |
|
Vehicle Repl. 6
|
hMEC
|
ligand: Ethanol
cell type: mammary epithelial cell
|
|
| Sample_geo_accession | GSM602712
| | Sample_status | Public on Oct 01 2010
| | Sample_submission_date | Sep 30 2010
| | Sample_last_update_date | Sep 30 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Adenoviruses overexpressing hPR-B or β-Gal were generated using the ViraPower Adenoviral Expression System (Invitrogen), and were amplified and purified by CsCl2 centrifugation. Cells were infected at a multiplicity of infection (MOI) of 150 for 17.5 hr. Following infection, cells were treated with 10nM R5020 or vehicle for 16 hours.
| | Sample_growth_protocol_ch1 | Human mammary epithelial cells were were maintained in MEBM (Lonza, Basel, Switzerland) supplemented with MEGM SingleQuots (Lonza) at 37 degrees C in 5% CO2 atmosphere
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated by using either RNeasy kit (Qiagen, Valencia, CA) or Aurum total RNA mini kit (Bio-Rad, Hercules, CA).
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Affymetrix 1-cycle IVT
| | Sample_hyb_protocol | standard Affymetrix protocol
| | Sample_scan_protocol | Affymetrix GeneChip scanner; Pixel size 1.56, Filter 570.
| | Sample_data_processing | Raw data were processed using JMP Genomics 4.1 (SAS). Background subtraction: RMA; Normalization: quantile; Transformation: Log2; Summarization: median polish.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Dmitri,A,Kazmin
| | Sample_contact_email | kazmi002@mc.duke.edu
| | Sample_contact_phone | 919-681-1348
| | Sample_contact_fax | 919-681-7139
| | Sample_contact_laboratory | McDonnell
| | Sample_contact_department | Pharmacology and Cancer Biology
| | Sample_contact_institute | Duke University Medical Center
| | Sample_contact_address | C264 LSRC, Research Dr
| | Sample_contact_city | Durham
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 27710
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM602nnn/GSM602712/suppl/GSM602712_1121_35247_H133+_12677_B6_-_@F2.CEL.gz
| | Sample_series_id | GSE24468
| | Sample_data_row_count | 54613
| | |
|
GSM602713 | GPL570 |
|
Vehicle Repl. 7
|
hMEC
|
ligand: Ethanol
cell type: mammary epithelial cell
|
|
| Sample_geo_accession | GSM602713
| | Sample_status | Public on Oct 01 2010
| | Sample_submission_date | Sep 30 2010
| | Sample_last_update_date | Sep 30 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Adenoviruses overexpressing hPR-B or β-Gal were generated using the ViraPower Adenoviral Expression System (Invitrogen), and were amplified and purified by CsCl2 centrifugation. Cells were infected at a multiplicity of infection (MOI) of 150 for 17.5 hr. Following infection, cells were treated with 10nM R5020 or vehicle for 16 hours.
| | Sample_growth_protocol_ch1 | Human mammary epithelial cells were were maintained in MEBM (Lonza, Basel, Switzerland) supplemented with MEGM SingleQuots (Lonza) at 37 degrees C in 5% CO2 atmosphere
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated by using either RNeasy kit (Qiagen, Valencia, CA) or Aurum total RNA mini kit (Bio-Rad, Hercules, CA).
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Affymetrix 1-cycle IVT
| | Sample_hyb_protocol | standard Affymetrix protocol
| | Sample_scan_protocol | Affymetrix GeneChip scanner; Pixel size 1.56, Filter 570.
| | Sample_data_processing | Raw data were processed using JMP Genomics 4.1 (SAS). Background subtraction: RMA; Normalization: quantile; Transformation: Log2; Summarization: median polish.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Dmitri,A,Kazmin
| | Sample_contact_email | kazmi002@mc.duke.edu
| | Sample_contact_phone | 919-681-1348
| | Sample_contact_fax | 919-681-7139
| | Sample_contact_laboratory | McDonnell
| | Sample_contact_department | Pharmacology and Cancer Biology
| | Sample_contact_institute | Duke University Medical Center
| | Sample_contact_address | C264 LSRC, Research Dr
| | Sample_contact_city | Durham
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 27710
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM602nnn/GSM602713/suppl/GSM602713_1121_35248_H133+_12678_B7_-_.CEL.gz
| | Sample_series_id | GSE24468
| | Sample_data_row_count | 54613
| | |
|
GSM602714 | GPL570 |
|
Vehicle Repl. 8
|
hMEC
|
ligand: Ethanol
cell type: mammary epithelial cell
|
|
| Sample_geo_accession | GSM602714
| | Sample_status | Public on Oct 01 2010
| | Sample_submission_date | Sep 30 2010
| | Sample_last_update_date | Sep 30 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Adenoviruses overexpressing hPR-B or β-Gal were generated using the ViraPower Adenoviral Expression System (Invitrogen), and were amplified and purified by CsCl2 centrifugation. Cells were infected at a multiplicity of infection (MOI) of 150 for 17.5 hr. Following infection, cells were treated with 10nM R5020 or vehicle for 16 hours.
| | Sample_growth_protocol_ch1 | Human mammary epithelial cells were were maintained in MEBM (Lonza, Basel, Switzerland) supplemented with MEGM SingleQuots (Lonza) at 37 degrees C in 5% CO2 atmosphere
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated by using either RNeasy kit (Qiagen, Valencia, CA) or Aurum total RNA mini kit (Bio-Rad, Hercules, CA).
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Affymetrix 1-cycle IVT
| | Sample_hyb_protocol | standard Affymetrix protocol
| | Sample_scan_protocol | Affymetrix GeneChip scanner; Pixel size 1.56, Filter 570.
| | Sample_data_processing | Raw data were processed using JMP Genomics 4.1 (SAS). Background subtraction: RMA; Normalization: quantile; Transformation: Log2; Summarization: median polish.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Dmitri,A,Kazmin
| | Sample_contact_email | kazmi002@mc.duke.edu
| | Sample_contact_phone | 919-681-1348
| | Sample_contact_fax | 919-681-7139
| | Sample_contact_laboratory | McDonnell
| | Sample_contact_department | Pharmacology and Cancer Biology
| | Sample_contact_institute | Duke University Medical Center
| | Sample_contact_address | C264 LSRC, Research Dr
| | Sample_contact_city | Durham
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 27710
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM602nnn/GSM602714/suppl/GSM602714_1121_35249_H133+_12679_B8_-_.CEL.gz
| | Sample_series_id | GSE24468
| | Sample_data_row_count | 54613
| | |
|
GSM602715 | GPL570 |
|
Vehicle Repl. 9
|
hMEC
|
ligand: Ethanol
cell type: mammary epithelial cell
|
|
| Sample_geo_accession | GSM602715
| | Sample_status | Public on Oct 01 2010
| | Sample_submission_date | Sep 30 2010
| | Sample_last_update_date | Sep 30 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Adenoviruses overexpressing hPR-B or β-Gal were generated using the ViraPower Adenoviral Expression System (Invitrogen), and were amplified and purified by CsCl2 centrifugation. Cells were infected at a multiplicity of infection (MOI) of 150 for 17.5 hr. Following infection, cells were treated with 10nM R5020 or vehicle for 16 hours.
| | Sample_growth_protocol_ch1 | Human mammary epithelial cells were were maintained in MEBM (Lonza, Basel, Switzerland) supplemented with MEGM SingleQuots (Lonza) at 37 degrees C in 5% CO2 atmosphere
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated by using either RNeasy kit (Qiagen, Valencia, CA) or Aurum total RNA mini kit (Bio-Rad, Hercules, CA).
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Affymetrix 1-cycle IVT
| | Sample_hyb_protocol | standard Affymetrix protocol
| | Sample_scan_protocol | Affymetrix GeneChip scanner; Pixel size 1.56, Filter 570.
| | Sample_data_processing | Raw data were processed using JMP Genomics 4.1 (SAS). Background subtraction: RMA; Normalization: quantile; Transformation: Log2; Summarization: median polish.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Dmitri,A,Kazmin
| | Sample_contact_email | kazmi002@mc.duke.edu
| | Sample_contact_phone | 919-681-1348
| | Sample_contact_fax | 919-681-7139
| | Sample_contact_laboratory | McDonnell
| | Sample_contact_department | Pharmacology and Cancer Biology
| | Sample_contact_institute | Duke University Medical Center
| | Sample_contact_address | C264 LSRC, Research Dr
| | Sample_contact_city | Durham
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 27710
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM602nnn/GSM602715/suppl/GSM602715_1121_35250_H133+_12680_B9_-_@F2.CEL.gz
| | Sample_series_id | GSE24468
| | Sample_data_row_count | 54613
| | |
|
GSM602716 | GPL570 |
|
Vehicle Repl. 10
|
hMEC
|
ligand: Ethanol
cell type: mammary epithelial cell
|
|
| Sample_geo_accession | GSM602716
| | Sample_status | Public on Oct 01 2010
| | Sample_submission_date | Sep 30 2010
| | Sample_last_update_date | Sep 30 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Adenoviruses overexpressing hPR-B or β-Gal were generated using the ViraPower Adenoviral Expression System (Invitrogen), and were amplified and purified by CsCl2 centrifugation. Cells were infected at a multiplicity of infection (MOI) of 150 for 17.5 hr. Following infection, cells were treated with 10nM R5020 or vehicle for 16 hours.
| | Sample_growth_protocol_ch1 | Human mammary epithelial cells were were maintained in MEBM (Lonza, Basel, Switzerland) supplemented with MEGM SingleQuots (Lonza) at 37 degrees C in 5% CO2 atmosphere
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated by using either RNeasy kit (Qiagen, Valencia, CA) or Aurum total RNA mini kit (Bio-Rad, Hercules, CA).
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Affymetrix 1-cycle IVT
| | Sample_hyb_protocol | standard Affymetrix protocol
| | Sample_scan_protocol | Affymetrix GeneChip scanner; Pixel size 1.56, Filter 570.
| | Sample_data_processing | Raw data were processed using JMP Genomics 4.1 (SAS). Background subtraction: RMA; Normalization: quantile; Transformation: Log2; Summarization: median polish.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Dmitri,A,Kazmin
| | Sample_contact_email | kazmi002@mc.duke.edu
| | Sample_contact_phone | 919-681-1348
| | Sample_contact_fax | 919-681-7139
| | Sample_contact_laboratory | McDonnell
| | Sample_contact_department | Pharmacology and Cancer Biology
| | Sample_contact_institute | Duke University Medical Center
| | Sample_contact_address | C264 LSRC, Research Dr
| | Sample_contact_city | Durham
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 27710
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM602nnn/GSM602716/suppl/GSM602716_1121_35251_H133+_12681_B10_-_.CEL.gz
| | Sample_series_id | GSE24468
| | Sample_data_row_count | 54613
| | |
|
| |
| |
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