Search results for the GEO ID: GSE24522  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM604738 | GPL570 |
|
MMS1 cell line, control, rep1
|
lentiviruses harboing GFP transduced MMS1 Cell lines
|
cell line: MMS1
cancer type: multiple myeloma
treatment: control
|
Gene Expression data from mutiple myoloma cell line MMS1
|
| Sample_geo_accession | GSM604738
| | Sample_status | Public on Oct 20 2010
| | Sample_submission_date | Oct 05 2010
| | Sample_last_update_date | Oct 19 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were previously transduced by lentiviruses harboing GFP and miR16 binding sponges (or control mock sponges), sorted by flow cytometry, and expanded.
| | Sample_growth_protocol_ch1 | Stably transduced RPMI 8226 and MMS1 Cell lines were evenly counted and grown in parallel flasks for a week until confluent, lysed at the same time point and RNA extracted for analysis.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted according to the manufacturer's protocol, purified using RNeasy kit (Qiagen)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 15ug total RNA was amplified by using Affymetrix GeneChip® Two-Cycle Target Labeling Reagents for 2 rounds of linear amplification and was labeled with biotin-conjugated nucleotides incorporated in the final in vitro transcription reaction.
| | Sample_hyb_protocol | The labeled cDNAs were dissolved in 200ul MES Hybridization Buffer and were hybridized with microarray for 16 h at 45°C.
| | Sample_scan_protocol | Then the microarrays were washed and scanned by an Affymetrix GeneChip Scanner 3000, 7G.
| | Sample_data_processing | The data were normalized with plier16 method, in log2 transformed scale.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Daniel,Ruben,Carrasco
| | Sample_contact_email | ruben_carrasco@dfci.harvard.edu
| | Sample_contact_laboratory | Carrasco
| | Sample_contact_department | Medical Oncology
| | Sample_contact_institute | Dana-Farber Cancer Institute
| | Sample_contact_address | 44 Binney St
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | MA 02115
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM604nnn/GSM604738/suppl/GSM604738.CEL.gz
| | Sample_series_id | GSE24522
| | Sample_data_row_count | 54675
| | |
|
GSM604739 | GPL570 |
|
MMS1 cell line, control, rep2
|
lentiviruses harboing GFP transduced MMS1 Cell lines
|
cell line: MMS1
cancer type: multiple myeloma
treatment: control
|
Gene Expression data from mutiple myoloma cell line MMS1
|
| Sample_geo_accession | GSM604739
| | Sample_status | Public on Oct 20 2010
| | Sample_submission_date | Oct 05 2010
| | Sample_last_update_date | Oct 19 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were previously transduced by lentiviruses harboing GFP and miR16 binding sponges (or control mock sponges), sorted by flow cytometry, and expanded.
| | Sample_growth_protocol_ch1 | Stably transduced RPMI 8226 and MMS1 Cell lines were evenly counted and grown in parallel flasks for a week until confluent, lysed at the same time point and RNA extracted for analysis.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted according to the manufacturer's protocol, purified using RNeasy kit (Qiagen)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 15ug total RNA was amplified by using Affymetrix GeneChip® Two-Cycle Target Labeling Reagents for 2 rounds of linear amplification and was labeled with biotin-conjugated nucleotides incorporated in the final in vitro transcription reaction.
| | Sample_hyb_protocol | The labeled cDNAs were dissolved in 200ul MES Hybridization Buffer and were hybridized with microarray for 16 h at 45°C.
| | Sample_scan_protocol | Then the microarrays were washed and scanned by an Affymetrix GeneChip Scanner 3000, 7G.
| | Sample_data_processing | The data were normalized with plier16 method, in log2 transformed scale.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Daniel,Ruben,Carrasco
| | Sample_contact_email | ruben_carrasco@dfci.harvard.edu
| | Sample_contact_laboratory | Carrasco
| | Sample_contact_department | Medical Oncology
| | Sample_contact_institute | Dana-Farber Cancer Institute
| | Sample_contact_address | 44 Binney St
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | MA 02115
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM604nnn/GSM604739/suppl/GSM604739.CEL.gz
| | Sample_series_id | GSE24522
| | Sample_data_row_count | 54675
| | |
|
GSM604740 | GPL570 |
|
MMS1 cell line, control, rep3
|
lentiviruses harboing GFP transduced MMS1 Cell lines
|
cell line: MMS1
cancer type: multiple myeloma
treatment: control
|
Gene Expression data from mutiple myoloma cell line MMS1
|
| Sample_geo_accession | GSM604740
| | Sample_status | Public on Oct 20 2010
| | Sample_submission_date | Oct 05 2010
| | Sample_last_update_date | Oct 19 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were previously transduced by lentiviruses harboing GFP and miR16 binding sponges (or control mock sponges), sorted by flow cytometry, and expanded.
| | Sample_growth_protocol_ch1 | Stably transduced RPMI 8226 and MMS1 Cell lines were evenly counted and grown in parallel flasks for a week until confluent, lysed at the same time point and RNA extracted for analysis.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted according to the manufacturer's protocol, purified using RNeasy kit (Qiagen)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 15ug total RNA was amplified by using Affymetrix GeneChip® Two-Cycle Target Labeling Reagents for 2 rounds of linear amplification and was labeled with biotin-conjugated nucleotides incorporated in the final in vitro transcription reaction.
| | Sample_hyb_protocol | The labeled cDNAs were dissolved in 200ul MES Hybridization Buffer and were hybridized with microarray for 16 h at 45°C.
| | Sample_scan_protocol | Then the microarrays were washed and scanned by an Affymetrix GeneChip Scanner 3000, 7G.
| | Sample_data_processing | The data were normalized with plier16 method, in log2 transformed scale.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Daniel,Ruben,Carrasco
| | Sample_contact_email | ruben_carrasco@dfci.harvard.edu
| | Sample_contact_laboratory | Carrasco
| | Sample_contact_department | Medical Oncology
| | Sample_contact_institute | Dana-Farber Cancer Institute
| | Sample_contact_address | 44 Binney St
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | MA 02115
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM604nnn/GSM604740/suppl/GSM604740.CEL.gz
| | Sample_series_id | GSE24522
| | Sample_data_row_count | 54675
| | |
|
GSM604741 | GPL570 |
|
MMS1 cell line, miR16 KO, rep1
|
lentiviruses harboing miR16 KO transduced MMS1 Cell lines
|
cell line: MMS1
cancer type: multiple myeloma
treatment: miR16 knock down
|
Gene Expression data from mutiple myoloma cell line MMS1 treated with miR16 siRNA
|
| Sample_geo_accession | GSM604741
| | Sample_status | Public on Oct 20 2010
| | Sample_submission_date | Oct 05 2010
| | Sample_last_update_date | Oct 19 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were previously transduced by lentiviruses harboing GFP and miR16 binding sponges (or control mock sponges), sorted by flow cytometry, and expanded.
| | Sample_growth_protocol_ch1 | Stably transduced RPMI 8226 and MMS1 Cell lines were evenly counted and grown in parallel flasks for a week until confluent, lysed at the same time point and RNA extracted for analysis.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted according to the manufacturer's protocol, purified using RNeasy kit (Qiagen)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 15ug total RNA was amplified by using Affymetrix GeneChip® Two-Cycle Target Labeling Reagents for 2 rounds of linear amplification and was labeled with biotin-conjugated nucleotides incorporated in the final in vitro transcription reaction.
| | Sample_hyb_protocol | The labeled cDNAs were dissolved in 200ul MES Hybridization Buffer and were hybridized with microarray for 16 h at 45°C.
| | Sample_scan_protocol | Then the microarrays were washed and scanned by an Affymetrix GeneChip Scanner 3000, 7G.
| | Sample_data_processing | The data were normalized with plier16 method, in log2 transformed scale.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Daniel,Ruben,Carrasco
| | Sample_contact_email | ruben_carrasco@dfci.harvard.edu
| | Sample_contact_laboratory | Carrasco
| | Sample_contact_department | Medical Oncology
| | Sample_contact_institute | Dana-Farber Cancer Institute
| | Sample_contact_address | 44 Binney St
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | MA 02115
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM604nnn/GSM604741/suppl/GSM604741.CEL.gz
| | Sample_series_id | GSE24522
| | Sample_data_row_count | 54675
| | |
|
GSM604742 | GPL570 |
|
MMS1 cell line, miR16 KO, rep2
|
lentiviruses harboing miR16 KO transduced MMS1 Cell lines
|
cell line: MMS1
cancer type: multiple myeloma
treatment: miR16 knock down
|
Gene Expression data from mutiple myoloma cell line MMS1 treated with miR16 siRNA
|
| Sample_geo_accession | GSM604742
| | Sample_status | Public on Oct 20 2010
| | Sample_submission_date | Oct 05 2010
| | Sample_last_update_date | Oct 19 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were previously transduced by lentiviruses harboing GFP and miR16 binding sponges (or control mock sponges), sorted by flow cytometry, and expanded.
| | Sample_growth_protocol_ch1 | Stably transduced RPMI 8226 and MMS1 Cell lines were evenly counted and grown in parallel flasks for a week until confluent, lysed at the same time point and RNA extracted for analysis.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted according to the manufacturer's protocol, purified using RNeasy kit (Qiagen)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 15ug total RNA was amplified by using Affymetrix GeneChip® Two-Cycle Target Labeling Reagents for 2 rounds of linear amplification and was labeled with biotin-conjugated nucleotides incorporated in the final in vitro transcription reaction.
| | Sample_hyb_protocol | The labeled cDNAs were dissolved in 200ul MES Hybridization Buffer and were hybridized with microarray for 16 h at 45°C.
| | Sample_scan_protocol | Then the microarrays were washed and scanned by an Affymetrix GeneChip Scanner 3000, 7G.
| | Sample_data_processing | The data were normalized with plier16 method, in log2 transformed scale.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Daniel,Ruben,Carrasco
| | Sample_contact_email | ruben_carrasco@dfci.harvard.edu
| | Sample_contact_laboratory | Carrasco
| | Sample_contact_department | Medical Oncology
| | Sample_contact_institute | Dana-Farber Cancer Institute
| | Sample_contact_address | 44 Binney St
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | MA 02115
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM604nnn/GSM604742/suppl/GSM604742.CEL.gz
| | Sample_series_id | GSE24522
| | Sample_data_row_count | 54675
| | |
|
GSM604743 | GPL570 |
|
MMS1 cell line, miR16 KO, rep3
|
lentiviruses harboing miR16 KO transduced MMS1 Cell lines
|
cell line: MMS1
cancer type: multiple myeloma
treatment: miR16 knock down
|
Gene Expression data from mutiple myoloma cell line MMS1 treated with miR16 siRNA
|
| Sample_geo_accession | GSM604743
| | Sample_status | Public on Oct 20 2010
| | Sample_submission_date | Oct 05 2010
| | Sample_last_update_date | Oct 19 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were previously transduced by lentiviruses harboing GFP and miR16 binding sponges (or control mock sponges), sorted by flow cytometry, and expanded.
| | Sample_growth_protocol_ch1 | Stably transduced RPMI 8226 and MMS1 Cell lines were evenly counted and grown in parallel flasks for a week until confluent, lysed at the same time point and RNA extracted for analysis.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted according to the manufacturer's protocol, purified using RNeasy kit (Qiagen)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 15ug total RNA was amplified by using Affymetrix GeneChip® Two-Cycle Target Labeling Reagents for 2 rounds of linear amplification and was labeled with biotin-conjugated nucleotides incorporated in the final in vitro transcription reaction.
| | Sample_hyb_protocol | The labeled cDNAs were dissolved in 200ul MES Hybridization Buffer and were hybridized with microarray for 16 h at 45°C.
| | Sample_scan_protocol | Then the microarrays were washed and scanned by an Affymetrix GeneChip Scanner 3000, 7G.
| | Sample_data_processing | The data were normalized with plier16 method, in log2 transformed scale.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Daniel,Ruben,Carrasco
| | Sample_contact_email | ruben_carrasco@dfci.harvard.edu
| | Sample_contact_laboratory | Carrasco
| | Sample_contact_department | Medical Oncology
| | Sample_contact_institute | Dana-Farber Cancer Institute
| | Sample_contact_address | 44 Binney St
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | MA 02115
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM604nnn/GSM604743/suppl/GSM604743.CEL.gz
| | Sample_series_id | GSE24522
| | Sample_data_row_count | 54675
| | |
|
GSM604744 | GPL570 |
|
RPMI cell line, control, rep1
|
lentiviruses harboing GFP transduced RPMI826 Cell lines
|
cell line: RPMI826
cancer type: multiple myeloma
treatment: control
|
Gene Expression data from mutiple myoloma cell line RPMI826
|
| Sample_geo_accession | GSM604744
| | Sample_status | Public on Oct 20 2010
| | Sample_submission_date | Oct 05 2010
| | Sample_last_update_date | Oct 19 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were previously transduced by lentiviruses harboing GFP and miR16 binding sponges (or control mock sponges), sorted by flow cytometry, and expanded.
| | Sample_growth_protocol_ch1 | Stably transduced RPMI 8226 and MMS1 Cell lines were evenly counted and grown in parallel flasks for a week until confluent, lysed at the same time point and RNA extracted for analysis.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted according to the manufacturer's protocol, purified using RNeasy kit (Qiagen)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 15ug total RNA was amplified by using Affymetrix GeneChip® Two-Cycle Target Labeling Reagents for 2 rounds of linear amplification and was labeled with biotin-conjugated nucleotides incorporated in the final in vitro transcription reaction.
| | Sample_hyb_protocol | The labeled cDNAs were dissolved in 200ul MES Hybridization Buffer and were hybridized with microarray for 16 h at 45°C.
| | Sample_scan_protocol | Then the microarrays were washed and scanned by an Affymetrix GeneChip Scanner 3000, 7G.
| | Sample_data_processing | The data were normalized with plier16 method, in log2 transformed scale.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Daniel,Ruben,Carrasco
| | Sample_contact_email | ruben_carrasco@dfci.harvard.edu
| | Sample_contact_laboratory | Carrasco
| | Sample_contact_department | Medical Oncology
| | Sample_contact_institute | Dana-Farber Cancer Institute
| | Sample_contact_address | 44 Binney St
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | MA 02115
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM604nnn/GSM604744/suppl/GSM604744.CEL.gz
| | Sample_series_id | GSE24522
| | Sample_data_row_count | 54675
| | |
|
GSM604745 | GPL570 |
|
RPMI cell line, control, rep2
|
lentiviruses harboing GFP transduced RPMI826 Cell lines
|
cell line: RPMI826
cancer type: multiple myeloma
treatment: control
|
Gene Expression data from mutiple myoloma cell line RPMI826
|
| Sample_geo_accession | GSM604745
| | Sample_status | Public on Oct 20 2010
| | Sample_submission_date | Oct 05 2010
| | Sample_last_update_date | Oct 19 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were previously transduced by lentiviruses harboing GFP and miR16 binding sponges (or control mock sponges), sorted by flow cytometry, and expanded.
| | Sample_growth_protocol_ch1 | Stably transduced RPMI 8226 and MMS1 Cell lines were evenly counted and grown in parallel flasks for a week until confluent, lysed at the same time point and RNA extracted for analysis.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted according to the manufacturer's protocol, purified using RNeasy kit (Qiagen)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 15ug total RNA was amplified by using Affymetrix GeneChip® Two-Cycle Target Labeling Reagents for 2 rounds of linear amplification and was labeled with biotin-conjugated nucleotides incorporated in the final in vitro transcription reaction.
| | Sample_hyb_protocol | The labeled cDNAs were dissolved in 200ul MES Hybridization Buffer and were hybridized with microarray for 16 h at 45°C.
| | Sample_scan_protocol | Then the microarrays were washed and scanned by an Affymetrix GeneChip Scanner 3000, 7G.
| | Sample_data_processing | The data were normalized with plier16 method, in log2 transformed scale.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Daniel,Ruben,Carrasco
| | Sample_contact_email | ruben_carrasco@dfci.harvard.edu
| | Sample_contact_laboratory | Carrasco
| | Sample_contact_department | Medical Oncology
| | Sample_contact_institute | Dana-Farber Cancer Institute
| | Sample_contact_address | 44 Binney St
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | MA 02115
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM604nnn/GSM604745/suppl/GSM604745.CEL.gz
| | Sample_series_id | GSE24522
| | Sample_data_row_count | 54675
| | |
|
GSM604746 | GPL570 |
|
RPMI cell line, control, rep3
|
lentiviruses harboing GFP transduced RPMI826 Cell lines
|
cell line: RPMI826
cancer type: multiple myeloma
treatment: control
|
Gene Expression data from mutiple myoloma cell line RPMI826
|
| Sample_geo_accession | GSM604746
| | Sample_status | Public on Oct 20 2010
| | Sample_submission_date | Oct 05 2010
| | Sample_last_update_date | Oct 19 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were previously transduced by lentiviruses harboing GFP and miR16 binding sponges (or control mock sponges), sorted by flow cytometry, and expanded.
| | Sample_growth_protocol_ch1 | Stably transduced RPMI 8226 and MMS1 Cell lines were evenly counted and grown in parallel flasks for a week until confluent, lysed at the same time point and RNA extracted for analysis.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted according to the manufacturer's protocol, purified using RNeasy kit (Qiagen)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 15ug total RNA was amplified by using Affymetrix GeneChip® Two-Cycle Target Labeling Reagents for 2 rounds of linear amplification and was labeled with biotin-conjugated nucleotides incorporated in the final in vitro transcription reaction.
| | Sample_hyb_protocol | The labeled cDNAs were dissolved in 200ul MES Hybridization Buffer and were hybridized with microarray for 16 h at 45°C.
| | Sample_scan_protocol | Then the microarrays were washed and scanned by an Affymetrix GeneChip Scanner 3000, 7G.
| | Sample_data_processing | The data were normalized with plier16 method, in log2 transformed scale.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Daniel,Ruben,Carrasco
| | Sample_contact_email | ruben_carrasco@dfci.harvard.edu
| | Sample_contact_laboratory | Carrasco
| | Sample_contact_department | Medical Oncology
| | Sample_contact_institute | Dana-Farber Cancer Institute
| | Sample_contact_address | 44 Binney St
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | MA 02115
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM604nnn/GSM604746/suppl/GSM604746.CEL.gz
| | Sample_series_id | GSE24522
| | Sample_data_row_count | 54675
| | |
|
GSM604747 | GPL570 |
|
RPMI cell line, miR16 KO, rep1
|
lentiviruses harboing miR16 KO transduced RPMI826 Cell lines
|
cell line: RPMI826
cancer type: multiple myeloma
treatment: miR16 knock down
|
Gene Expression data from mutiple myoloma cell line RPMI826 treated with miR16 siRNA
|
| Sample_geo_accession | GSM604747
| | Sample_status | Public on Oct 20 2010
| | Sample_submission_date | Oct 05 2010
| | Sample_last_update_date | Oct 19 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were previously transduced by lentiviruses harboing GFP and miR16 binding sponges (or control mock sponges), sorted by flow cytometry, and expanded.
| | Sample_growth_protocol_ch1 | Stably transduced RPMI 8226 and MMS1 Cell lines were evenly counted and grown in parallel flasks for a week until confluent, lysed at the same time point and RNA extracted for analysis.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted according to the manufacturer's protocol, purified using RNeasy kit (Qiagen)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 15ug total RNA was amplified by using Affymetrix GeneChip® Two-Cycle Target Labeling Reagents for 2 rounds of linear amplification and was labeled with biotin-conjugated nucleotides incorporated in the final in vitro transcription reaction.
| | Sample_hyb_protocol | The labeled cDNAs were dissolved in 200ul MES Hybridization Buffer and were hybridized with microarray for 16 h at 45°C.
| | Sample_scan_protocol | Then the microarrays were washed and scanned by an Affymetrix GeneChip Scanner 3000, 7G.
| | Sample_data_processing | The data were normalized with plier16 method, in log2 transformed scale.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Daniel,Ruben,Carrasco
| | Sample_contact_email | ruben_carrasco@dfci.harvard.edu
| | Sample_contact_laboratory | Carrasco
| | Sample_contact_department | Medical Oncology
| | Sample_contact_institute | Dana-Farber Cancer Institute
| | Sample_contact_address | 44 Binney St
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | MA 02115
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM604nnn/GSM604747/suppl/GSM604747.CEL.gz
| | Sample_series_id | GSE24522
| | Sample_data_row_count | 54675
| | |
|
GSM604748 | GPL570 |
|
RPMI cell line, miR16 KO, rep2
|
lentiviruses harboing miR16 KO transduced RPMI826 Cell lines
|
cell line: RPMI826
cancer type: multiple myeloma
treatment: miR16 knock down
|
Gene Expression data from mutiple myoloma cell line RPMI826 treated with miR16 siRNA
|
| Sample_geo_accession | GSM604748
| | Sample_status | Public on Oct 20 2010
| | Sample_submission_date | Oct 05 2010
| | Sample_last_update_date | Oct 19 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were previously transduced by lentiviruses harboing GFP and miR16 binding sponges (or control mock sponges), sorted by flow cytometry, and expanded.
| | Sample_growth_protocol_ch1 | Stably transduced RPMI 8226 and MMS1 Cell lines were evenly counted and grown in parallel flasks for a week until confluent, lysed at the same time point and RNA extracted for analysis.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted according to the manufacturer's protocol, purified using RNeasy kit (Qiagen)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 15ug total RNA was amplified by using Affymetrix GeneChip® Two-Cycle Target Labeling Reagents for 2 rounds of linear amplification and was labeled with biotin-conjugated nucleotides incorporated in the final in vitro transcription reaction.
| | Sample_hyb_protocol | The labeled cDNAs were dissolved in 200ul MES Hybridization Buffer and were hybridized with microarray for 16 h at 45°C.
| | Sample_scan_protocol | Then the microarrays were washed and scanned by an Affymetrix GeneChip Scanner 3000, 7G.
| | Sample_data_processing | The data were normalized with plier16 method, in log2 transformed scale.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Daniel,Ruben,Carrasco
| | Sample_contact_email | ruben_carrasco@dfci.harvard.edu
| | Sample_contact_laboratory | Carrasco
| | Sample_contact_department | Medical Oncology
| | Sample_contact_institute | Dana-Farber Cancer Institute
| | Sample_contact_address | 44 Binney St
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | MA 02115
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM604nnn/GSM604748/suppl/GSM604748.CEL.gz
| | Sample_series_id | GSE24522
| | Sample_data_row_count | 54675
| | |
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GSM604749 | GPL570 |
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RPMI cell line, miR16 KO, rep3
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lentiviruses harboing miR16 KO transduced RPMI826 Cell lines
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cell line: RPMI826
cancer type: multiple myeloma
treatment: miR16 knock down
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Gene Expression data from mutiple myoloma cell line RPMI826 treated with miR16 siRNA
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| Sample_geo_accession | GSM604749
| | Sample_status | Public on Oct 20 2010
| | Sample_submission_date | Oct 05 2010
| | Sample_last_update_date | Oct 19 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were previously transduced by lentiviruses harboing GFP and miR16 binding sponges (or control mock sponges), sorted by flow cytometry, and expanded.
| | Sample_growth_protocol_ch1 | Stably transduced RPMI 8226 and MMS1 Cell lines were evenly counted and grown in parallel flasks for a week until confluent, lysed at the same time point and RNA extracted for analysis.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was extracted according to the manufacturer's protocol, purified using RNeasy kit (Qiagen)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | 15ug total RNA was amplified by using Affymetrix GeneChip® Two-Cycle Target Labeling Reagents for 2 rounds of linear amplification and was labeled with biotin-conjugated nucleotides incorporated in the final in vitro transcription reaction.
| | Sample_hyb_protocol | The labeled cDNAs were dissolved in 200ul MES Hybridization Buffer and were hybridized with microarray for 16 h at 45°C.
| | Sample_scan_protocol | Then the microarrays were washed and scanned by an Affymetrix GeneChip Scanner 3000, 7G.
| | Sample_data_processing | The data were normalized with plier16 method, in log2 transformed scale.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Daniel,Ruben,Carrasco
| | Sample_contact_email | ruben_carrasco@dfci.harvard.edu
| | Sample_contact_laboratory | Carrasco
| | Sample_contact_department | Medical Oncology
| | Sample_contact_institute | Dana-Farber Cancer Institute
| | Sample_contact_address | 44 Binney St
| | Sample_contact_city | Boston
| | Sample_contact_state | MA
| | Sample_contact_zip/postal_code | MA 02115
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM604nnn/GSM604749/suppl/GSM604749.CEL.gz
| | Sample_series_id | GSE24522
| | Sample_data_row_count | 54675
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