Search results for the GEO ID: GSE24584  |
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GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM606061 | GPL570 |
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CL1-5 cells treated Analogue 3, 4′-iodo-3,3,5-tripropyl-4-methoxy
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human lung cancer cell line treated Analogue 3 as test
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treatment: 4′-iodo-3,3,5-tripropyl-4-methoxy
cell line: CL1-5
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Gene expression data from CL1-5 treated with Analogue 3
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| Sample_geo_accession | GSM606061
| | Sample_status | Public on Jan 03 2011
| | Sample_submission_date | Oct 07 2010
| | Sample_last_update_date | Jan 03 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | 2.5 × 105 CL1-5 cells were treated for 24 h with 0.11 microM Analogue 3 or DMSO at a concentration of 0.05 µg/mL
| | Sample_growth_protocol_ch1 | Human lung adenocarcinoma CL1-5 cells were grown in DMEM with 10% fetal bovine serum at 37°C, 20% O2, 5% CO2.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 12 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix)(Santa Clara, CA, USA, http://www.affymetrix.com).
| | Sample_hyb_protocol | Following fragmentation, 20 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Human Genome 133 plus 2.0 GeneChip on Affymetrix Hybridization Oven 645, 60 RPM, then washed and stained in the Affymetrix GeneChip Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were processed by GeneSpring GX software version 11 GC-RMA preprocessor to use on the CEL files
| | Sample_platform_id | GPL570
| | Sample_contact_name | Sung-Liang,,Yu
| | Sample_contact_email | slyu@ntu.edu.tw
| | Sample_contact_phone | 886-2-23958341
| | Sample_contact_fax | 886-2-23958341
| | Sample_contact_laboratory | Microarray Core Facility
| | Sample_contact_department | Clinical Laboratory Sciences and Medical Biotechnology
| | Sample_contact_institute | National Taiwan University
| | Sample_contact_address | Jen Ai Road Section1
| | Sample_contact_city | Taipei
| | Sample_contact_zip/postal_code | 100
| | Sample_contact_country | Taiwan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM606nnn/GSM606061/suppl/GSM606061_CL1-5_411C2.CEL.gz
| | Sample_series_id | GSE24584
| | Sample_data_row_count | 54675
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GSM606062 | GPL570 |
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CL1-5 cells treated DMSO
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human lung cancer cell line treated DMSO as control
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treatment: control
cell line: CL1-5
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Gene expression data from CL1-5 treated with DMSO
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| Sample_geo_accession | GSM606062
| | Sample_status | Public on Jan 03 2011
| | Sample_submission_date | Oct 07 2010
| | Sample_last_update_date | Jan 03 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | 2.5 × 105 CL1-5 cells were treated for 24 h with 0.11 microM Analogue 3 or DMSO at a concentration of 0.05 µg/mL
| | Sample_growth_protocol_ch1 | Human lung adenocarcinoma CL1-5 cells were grown in DMEM with 10% fetal bovine serum at 37°C, 20% O2, 5% CO2.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 12 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix)(Santa Clara, CA, USA, http://www.affymetrix.com).
| | Sample_hyb_protocol | Following fragmentation, 20 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Human Genome 133 plus 2.0 GeneChip on Affymetrix Hybridization Oven 645, 60 RPM, then washed and stained in the Affymetrix GeneChip Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were processed by GeneSpring GX software version 11 GC-RMA preprocessor to use on the CEL files
| | Sample_platform_id | GPL570
| | Sample_contact_name | Sung-Liang,,Yu
| | Sample_contact_email | slyu@ntu.edu.tw
| | Sample_contact_phone | 886-2-23958341
| | Sample_contact_fax | 886-2-23958341
| | Sample_contact_laboratory | Microarray Core Facility
| | Sample_contact_department | Clinical Laboratory Sciences and Medical Biotechnology
| | Sample_contact_institute | National Taiwan University
| | Sample_contact_address | Jen Ai Road Section1
| | Sample_contact_city | Taipei
| | Sample_contact_zip/postal_code | 100
| | Sample_contact_country | Taiwan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM606nnn/GSM606062/suppl/GSM606062_CL1-5_DMSO.CEL.gz
| | Sample_series_id | GSE24584
| | Sample_data_row_count | 54675
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