Result

Search results for the GEO ID: GSE24683

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GSM ID

GPL ID

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Title

Source name

Description

Characteristics

GSM608130

GPL1261

Control preadipocytes, biological rep1

Subcutaneous preadipocytes, control

genetic background: C57BL/6 genotype: Dicer lox/lox cell type: Subcutaneous preadipocytes cell source: Isolated from day 2 newborns treatment: control

Sample_geo_accession	GSM608130
Sample_status	Public on Mar 31 2012
Sample_submission_date	Oct 13 2010
Sample_last_update_date	Mar 31 2012
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Cells were transduced with 750 MOI adenoviruses harboring GFP (Ad5CMVeGFP) or CRE recombinase (Ad5CMVCre-eGFP) (Gene Transfer Vector Core, University of Iowa) in the presence of 4 μg/mL polybrene. Four days after adenovirus infection, Dicer knockout cells or controls were harvested for RNA isolation.
Sample_growth_protocol_ch1	Cells were grown in DMEM (Invitrogen) with 10% fetal bovine serum (BenchMark, Gemini) in a humidified 5% CO2 atmosphere at 37 °C. Penicillin/Streptomycin (Invitrogen) and Normocin (Invivogen) was supplemented to the culture medium.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated aRNA synthesis was performed using the MessageAmp III aRNA Amplification Kit (Ambion)
Sample_hyb_protocol	6µg of biotinylated cRNA were hybridized for 16hr at 45C to the Affymetrix Mouse Genome 430 2.0 array. Following hybridization, arrays were washed and stained on the Affymetrix Fluidics Station 450.
Sample_scan_protocol	GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
Sample_data_processing	Data were analyzed using Affymetrix GCOS v. 1.3, MAS5.0 algorithm, and globally scaled with the trimmed mean target intensity set to 1500.
Sample_platform_id	GPL1261
Sample_contact_name	Marcelo,A.,Mori
Sample_contact_email	marcelo.mori@joslin.harvard.edu
Sample_contact_institute	Joslin Diabetes Center
Sample_contact_address	1 Joslin Place
Sample_contact_city	Boston
Sample_contact_state	MA
Sample_contact_zip/postal_code	02215
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM608nnn/GSM608130/suppl/GSM608130_Lox_1.CEL.gz
Sample_series_id	GSE24683
Sample_data_row_count	45101

GSM608131

GPL1261

Control preadipocytes, biological rep2

Subcutaneous preadipocytes, control

genetic background: C57BL/6 genotype: Dicer lox/lox cell type: Subcutaneous preadipocytes cell source: Isolated from day 2 newborns treatment: control

Sample_geo_accession	GSM608131
Sample_status	Public on Mar 31 2012
Sample_submission_date	Oct 13 2010
Sample_last_update_date	Mar 31 2012
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Cells were transduced with 750 MOI adenoviruses harboring GFP (Ad5CMVeGFP) or CRE recombinase (Ad5CMVCre-eGFP) (Gene Transfer Vector Core, University of Iowa) in the presence of 4 μg/mL polybrene. Four days after adenovirus infection, Dicer knockout cells or controls were harvested for RNA isolation.
Sample_growth_protocol_ch1	Cells were grown in DMEM (Invitrogen) with 10% fetal bovine serum (BenchMark, Gemini) in a humidified 5% CO2 atmosphere at 37 °C. Penicillin/Streptomycin (Invitrogen) and Normocin (Invivogen) was supplemented to the culture medium.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated aRNA synthesis was performed using the MessageAmp III aRNA Amplification Kit (Ambion)
Sample_hyb_protocol	6µg of biotinylated cRNA were hybridized for 16hr at 45C to the Affymetrix Mouse Genome 430 2.0 array. Following hybridization, arrays were washed and stained on the Affymetrix Fluidics Station 450.
Sample_scan_protocol	GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
Sample_data_processing	Data were analyzed using Affymetrix GCOS v. 1.3, MAS5.0 algorithm, and globally scaled with the trimmed mean target intensity set to 1500.
Sample_platform_id	GPL1261
Sample_contact_name	Marcelo,A.,Mori
Sample_contact_email	marcelo.mori@joslin.harvard.edu
Sample_contact_institute	Joslin Diabetes Center
Sample_contact_address	1 Joslin Place
Sample_contact_city	Boston
Sample_contact_state	MA
Sample_contact_zip/postal_code	02215
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM608nnn/GSM608131/suppl/GSM608131_Lox_2.CEL.gz
Sample_series_id	GSE24683
Sample_data_row_count	45101

GSM608132

GPL1261

Control preadipocytes, biological rep3

Subcutaneous preadipocytes, control

genetic background: C57BL/6 genotype: Dicer lox/lox cell type: Subcutaneous preadipocytes cell source: Isolated from day 2 newborns treatment: control

Sample_geo_accession	GSM608132
Sample_status	Public on Mar 31 2012
Sample_submission_date	Oct 13 2010
Sample_last_update_date	Mar 31 2012
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Cells were transduced with 750 MOI adenoviruses harboring GFP (Ad5CMVeGFP) or CRE recombinase (Ad5CMVCre-eGFP) (Gene Transfer Vector Core, University of Iowa) in the presence of 4 μg/mL polybrene. Four days after adenovirus infection, Dicer knockout cells or controls were harvested for RNA isolation.
Sample_growth_protocol_ch1	Cells were grown in DMEM (Invitrogen) with 10% fetal bovine serum (BenchMark, Gemini) in a humidified 5% CO2 atmosphere at 37 °C. Penicillin/Streptomycin (Invitrogen) and Normocin (Invivogen) was supplemented to the culture medium.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated aRNA synthesis was performed using the MessageAmp III aRNA Amplification Kit (Ambion)
Sample_hyb_protocol	6µg of biotinylated cRNA were hybridized for 16hr at 45C to the Affymetrix Mouse Genome 430 2.0 array. Following hybridization, arrays were washed and stained on the Affymetrix Fluidics Station 450.
Sample_scan_protocol	GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
Sample_data_processing	Data were analyzed using Affymetrix GCOS v. 1.3, MAS5.0 algorithm, and globally scaled with the trimmed mean target intensity set to 1500.
Sample_platform_id	GPL1261
Sample_contact_name	Marcelo,A.,Mori
Sample_contact_email	marcelo.mori@joslin.harvard.edu
Sample_contact_institute	Joslin Diabetes Center
Sample_contact_address	1 Joslin Place
Sample_contact_city	Boston
Sample_contact_state	MA
Sample_contact_zip/postal_code	02215
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM608nnn/GSM608132/suppl/GSM608132_Lox_3.CEL.gz
Sample_series_id	GSE24683
Sample_data_row_count	45101

GSM608133

GPL1261

Control preadipocytes, biological rep4

Subcutaneous preadipocytes, control

genetic background: C57BL/6 genotype: Dicer lox/lox cell type: Subcutaneous preadipocytes cell source: Isolated from day 2 newborns treatment: control

Sample_geo_accession	GSM608133
Sample_status	Public on Mar 31 2012
Sample_submission_date	Oct 13 2010
Sample_last_update_date	Mar 31 2012
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Cells were transduced with 750 MOI adenoviruses harboring GFP (Ad5CMVeGFP) or CRE recombinase (Ad5CMVCre-eGFP) (Gene Transfer Vector Core, University of Iowa) in the presence of 4 μg/mL polybrene. Four days after adenovirus infection, Dicer knockout cells or controls were harvested for RNA isolation.
Sample_growth_protocol_ch1	Cells were grown in DMEM (Invitrogen) with 10% fetal bovine serum (BenchMark, Gemini) in a humidified 5% CO2 atmosphere at 37 °C. Penicillin/Streptomycin (Invitrogen) and Normocin (Invivogen) was supplemented to the culture medium.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated aRNA synthesis was performed using the MessageAmp III aRNA Amplification Kit (Ambion)
Sample_hyb_protocol	6µg of biotinylated cRNA were hybridized for 16hr at 45C to the Affymetrix Mouse Genome 430 2.0 array. Following hybridization, arrays were washed and stained on the Affymetrix Fluidics Station 450.
Sample_scan_protocol	GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
Sample_data_processing	Data were analyzed using Affymetrix GCOS v. 1.3, MAS5.0 algorithm, and globally scaled with the trimmed mean target intensity set to 1500.
Sample_platform_id	GPL1261
Sample_contact_name	Marcelo,A.,Mori
Sample_contact_email	marcelo.mori@joslin.harvard.edu
Sample_contact_institute	Joslin Diabetes Center
Sample_contact_address	1 Joslin Place
Sample_contact_city	Boston
Sample_contact_state	MA
Sample_contact_zip/postal_code	02215
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM608nnn/GSM608133/suppl/GSM608133_Lox_4.CEL.gz
Sample_series_id	GSE24683
Sample_data_row_count	45101

GSM608134

GPL1261

Dicer knockout preadipocytes, biological rep1

Subcutaneous preadipocytes, Dicer knockout

genetic background: C57BL/6 genotype: Dicer lox/lox cell type: Subcutaneous preadipocytes cell source: Isolated from day 2 newborns treatment: Dicer ablation

Sample_geo_accession	GSM608134
Sample_status	Public on Mar 31 2012
Sample_submission_date	Oct 13 2010
Sample_last_update_date	Mar 31 2012
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Cells were transduced with 750 MOI adenoviruses harboring GFP (Ad5CMVeGFP) or CRE recombinase (Ad5CMVCre-eGFP) (Gene Transfer Vector Core, University of Iowa) in the presence of 4 μg/mL polybrene. Four days after adenovirus infection, Dicer knockout cells or controls were harvested for RNA isolation.
Sample_growth_protocol_ch1	Cells were grown in DMEM (Invitrogen) with 10% fetal bovine serum (BenchMark, Gemini) in a humidified 5% CO2 atmosphere at 37 °C. Penicillin/Streptomycin (Invitrogen) and Normocin (Invivogen) was supplemented to the culture medium.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated aRNA synthesis was performed using the MessageAmp III aRNA Amplification Kit (Ambion)
Sample_hyb_protocol	6µg of biotinylated cRNA were hybridized for 16hr at 45C to the Affymetrix Mouse Genome 430 2.0 array. Following hybridization, arrays were washed and stained on the Affymetrix Fluidics Station 450.
Sample_scan_protocol	GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
Sample_data_processing	Data were analyzed using Affymetrix GCOS v. 1.3, MAS5.0 algorithm, and globally scaled with the trimmed mean target intensity set to 1500.
Sample_platform_id	GPL1261
Sample_contact_name	Marcelo,A.,Mori
Sample_contact_email	marcelo.mori@joslin.harvard.edu
Sample_contact_institute	Joslin Diabetes Center
Sample_contact_address	1 Joslin Place
Sample_contact_city	Boston
Sample_contact_state	MA
Sample_contact_zip/postal_code	02215
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM608nnn/GSM608134/suppl/GSM608134_KO_1.CEL.gz
Sample_series_id	GSE24683
Sample_data_row_count	45101

GSM608135

GPL1261

Dicer knockout preadipocytes, biological rep2

Subcutaneous preadipocytes, Dicer knockout

genetic background: C57BL/6 genotype: Dicer lox/lox cell type: Subcutaneous preadipocytes cell source: Isolated from day 2 newborns treatment: Dicer ablation

Sample_geo_accession	GSM608135
Sample_status	Public on Mar 31 2012
Sample_submission_date	Oct 13 2010
Sample_last_update_date	Mar 31 2012
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Cells were transduced with 750 MOI adenoviruses harboring GFP (Ad5CMVeGFP) or CRE recombinase (Ad5CMVCre-eGFP) (Gene Transfer Vector Core, University of Iowa) in the presence of 4 μg/mL polybrene. Four days after adenovirus infection, Dicer knockout cells or controls were harvested for RNA isolation.
Sample_growth_protocol_ch1	Cells were grown in DMEM (Invitrogen) with 10% fetal bovine serum (BenchMark, Gemini) in a humidified 5% CO2 atmosphere at 37 °C. Penicillin/Streptomycin (Invitrogen) and Normocin (Invivogen) was supplemented to the culture medium.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated aRNA synthesis was performed using the MessageAmp III aRNA Amplification Kit (Ambion)
Sample_hyb_protocol	6µg of biotinylated cRNA were hybridized for 16hr at 45C to the Affymetrix Mouse Genome 430 2.0 array. Following hybridization, arrays were washed and stained on the Affymetrix Fluidics Station 450.
Sample_scan_protocol	GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
Sample_data_processing	Data were analyzed using Affymetrix GCOS v. 1.3, MAS5.0 algorithm, and globally scaled with the trimmed mean target intensity set to 1500.
Sample_platform_id	GPL1261
Sample_contact_name	Marcelo,A.,Mori
Sample_contact_email	marcelo.mori@joslin.harvard.edu
Sample_contact_institute	Joslin Diabetes Center
Sample_contact_address	1 Joslin Place
Sample_contact_city	Boston
Sample_contact_state	MA
Sample_contact_zip/postal_code	02215
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM608nnn/GSM608135/suppl/GSM608135_KO_2.CEL.gz
Sample_series_id	GSE24683
Sample_data_row_count	45101

GSM608136

GPL1261

Dicer knockout preadipocytes, biological rep3

Subcutaneous preadipocytes, Dicer knockout

genetic background: C57BL/6 genotype: Dicer lox/lox cell type: Subcutaneous preadipocytes cell source: Isolated from day 2 newborns treatment: Dicer ablation

Sample_geo_accession	GSM608136
Sample_status	Public on Mar 31 2012
Sample_submission_date	Oct 13 2010
Sample_last_update_date	Mar 31 2012
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Cells were transduced with 750 MOI adenoviruses harboring GFP (Ad5CMVeGFP) or CRE recombinase (Ad5CMVCre-eGFP) (Gene Transfer Vector Core, University of Iowa) in the presence of 4 μg/mL polybrene. Four days after adenovirus infection, Dicer knockout cells or controls were harvested for RNA isolation.
Sample_growth_protocol_ch1	Cells were grown in DMEM (Invitrogen) with 10% fetal bovine serum (BenchMark, Gemini) in a humidified 5% CO2 atmosphere at 37 °C. Penicillin/Streptomycin (Invitrogen) and Normocin (Invivogen) was supplemented to the culture medium.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated aRNA synthesis was performed using the MessageAmp III aRNA Amplification Kit (Ambion)
Sample_hyb_protocol	6µg of biotinylated cRNA were hybridized for 16hr at 45C to the Affymetrix Mouse Genome 430 2.0 array. Following hybridization, arrays were washed and stained on the Affymetrix Fluidics Station 450.
Sample_scan_protocol	GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
Sample_data_processing	Data were analyzed using Affymetrix GCOS v. 1.3, MAS5.0 algorithm, and globally scaled with the trimmed mean target intensity set to 1500.
Sample_platform_id	GPL1261
Sample_contact_name	Marcelo,A.,Mori
Sample_contact_email	marcelo.mori@joslin.harvard.edu
Sample_contact_institute	Joslin Diabetes Center
Sample_contact_address	1 Joslin Place
Sample_contact_city	Boston
Sample_contact_state	MA
Sample_contact_zip/postal_code	02215
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM608nnn/GSM608136/suppl/GSM608136_KO_3.CEL.gz
Sample_series_id	GSE24683
Sample_data_row_count	45101

GSM608137

GPL1261

Dicer knockout preadipocytes, biological rep4

Subcutaneous preadipocytes, Dicer knockout

genetic background: C57BL/6 genotype: Dicer lox/lox cell type: Subcutaneous preadipocytes cell source: Isolated from day 2 newborns treatment: Dicer ablation

Sample_geo_accession	GSM608137
Sample_status	Public on Mar 31 2012
Sample_submission_date	Oct 13 2010
Sample_last_update_date	Mar 31 2012
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Cells were transduced with 750 MOI adenoviruses harboring GFP (Ad5CMVeGFP) or CRE recombinase (Ad5CMVCre-eGFP) (Gene Transfer Vector Core, University of Iowa) in the presence of 4 μg/mL polybrene. Four days after adenovirus infection, Dicer knockout cells or controls were harvested for RNA isolation.
Sample_growth_protocol_ch1	Cells were grown in DMEM (Invitrogen) with 10% fetal bovine serum (BenchMark, Gemini) in a humidified 5% CO2 atmosphere at 37 °C. Penicillin/Streptomycin (Invitrogen) and Normocin (Invivogen) was supplemented to the culture medium.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated aRNA synthesis was performed using the MessageAmp III aRNA Amplification Kit (Ambion)
Sample_hyb_protocol	6µg of biotinylated cRNA were hybridized for 16hr at 45C to the Affymetrix Mouse Genome 430 2.0 array. Following hybridization, arrays were washed and stained on the Affymetrix Fluidics Station 450.
Sample_scan_protocol	GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
Sample_data_processing	Data were analyzed using Affymetrix GCOS v. 1.3, MAS5.0 algorithm, and globally scaled with the trimmed mean target intensity set to 1500.
Sample_platform_id	GPL1261
Sample_contact_name	Marcelo,A.,Mori
Sample_contact_email	marcelo.mori@joslin.harvard.edu
Sample_contact_institute	Joslin Diabetes Center
Sample_contact_address	1 Joslin Place
Sample_contact_city	Boston
Sample_contact_state	MA
Sample_contact_zip/postal_code	02215
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM608nnn/GSM608137/suppl/GSM608137_KO_4.CEL.gz
Sample_series_id	GSE24683
Sample_data_row_count	45101

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