Result

Search results for the GEO ID: GSE24726

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GSM ID

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Title

Source name

Description

Characteristics

GSM609039

GPL1261

PDC_control_day4_rep1

Ex vivo sorted plasmacytoid dendritic cells (PDC)

strain: C57BL/6 genotype/varation: control time point: day4 tissue: spleen cell type: PDC agent: tamoxifen

Sample_geo_accession	GSM609039
Sample_status	Public on Dec 21 2010
Sample_submission_date	Oct 15 2010
Sample_last_update_date	Dec 21 2010
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Inducible deletion of E2-2 (Tcf4) has been performed by administering tamoxifen to conditional E2-2flox/flox Rosa26-CreER+ mice or to E2-2flox/flox Rosa26-CreER- control littermates. Four days later total splenocytes were isolated, pooled from 2-3 mice and enriched for lineage (CD3/CD19)-negative cells by magnetic selection. PDC (CD11b- B220+ CD11clow Bst2+) were isolated using BD FACSAria II flow sorter directly into Trizol reagent.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was extracted using Trizol reagent (Invitrogen).
Sample_label_ch1	biotin
Sample_label_protocol_ch1	The aRNA was amplified without labeling in the first round, and then labeled with Biotin-16-UTP and Biotin-11-CTP in the second round. Labeled aRNA was fragmented in RNA Fragmentation Buffer (QIAGEN) prior to hybridization.
Sample_hyb_protocol	Labeled RNA samples (10 ug/array) were hybridized at the HICCC Genomics Facility of the Columbia University according to the manufacturer's instructions.
Sample_scan_protocol	Array scanning and raw data processing were done using GCOS 1.4 software (Affymetrix).
Sample_data_processing	MAS5.0
Sample_platform_id	GPL1261
Sample_contact_name	Boris,,Reizis
Sample_contact_email	bvr2101@columbia.edu
Sample_contact_department	Microbiology and Immunology
Sample_contact_institute	Columbia University Medical Center
Sample_contact_address	701 W 168th St.
Sample_contact_city	New York
Sample_contact_state	NY
Sample_contact_zip/postal_code	10032
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM609nnn/GSM609039/suppl/GSM609039.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM609nnn/GSM609039/suppl/GSM609039.CHP.gz
Sample_series_id	GSE24726
Sample_series_id	GSE24785
Sample_data_row_count	45101

GSM609040

GPL1261

PDC_control_day4_rep2

Ex vivo sorted plasmacytoid dendritic cells (PDC)

strain: C57BL/6 genotype/varation: control time point: day4 tissue: spleen cell type: PDC agent: tamoxifen

Sample_geo_accession	GSM609040
Sample_status	Public on Dec 21 2010
Sample_submission_date	Oct 15 2010
Sample_last_update_date	Dec 21 2010
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Inducible deletion of E2-2 (Tcf4) has been performed by administering tamoxifen to conditional E2-2flox/flox Rosa26-CreER+ mice or to E2-2flox/flox Rosa26-CreER- control littermates. Four days later total splenocytes were isolated, pooled from 2-3 mice and enriched for lineage (CD3/CD19)-negative cells by magnetic selection. PDC (CD11b- B220+ CD11clow Bst2+) were isolated using BD FACSAria II flow sorter directly into Trizol reagent.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was extracted using Trizol reagent (Invitrogen).
Sample_label_ch1	biotin
Sample_label_protocol_ch1	The aRNA was amplified without labeling in the first round, and then labeled with Biotin-16-UTP and Biotin-11-CTP in the second round. Labeled aRNA was fragmented in RNA Fragmentation Buffer (QIAGEN) prior to hybridization.
Sample_hyb_protocol	Labeled RNA samples (10 ug/array) were hybridized at the HICCC Genomics Facility of the Columbia University according to the manufacturer's instructions.
Sample_scan_protocol	Array scanning and raw data processing were done using GCOS 1.4 software (Affymetrix).
Sample_data_processing	MAS5.0
Sample_platform_id	GPL1261
Sample_contact_name	Boris,,Reizis
Sample_contact_email	bvr2101@columbia.edu
Sample_contact_department	Microbiology and Immunology
Sample_contact_institute	Columbia University Medical Center
Sample_contact_address	701 W 168th St.
Sample_contact_city	New York
Sample_contact_state	NY
Sample_contact_zip/postal_code	10032
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM609nnn/GSM609040/suppl/GSM609040.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM609nnn/GSM609040/suppl/GSM609040.CHP.gz
Sample_series_id	GSE24726
Sample_series_id	GSE24785
Sample_data_row_count	45101

GSM609041

GPL1261

PDC_control_day6_rep1

Ex vivo sorted plasmacytoid dendritic cells (PDC)

strain: C57BL/6 genotype/varation: control time point: day6 tissue: spleen cell type: PDC agent: tamoxifen

Sample_geo_accession	GSM609041
Sample_status	Public on Dec 21 2010
Sample_submission_date	Oct 15 2010
Sample_last_update_date	Dec 21 2010
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Inducible deletion of E2-2 (Tcf4) has been performed by administering tamoxifen to conditional E2-2flox/flox Rosa26-CreER+ mice or to E2-2flox/flox Rosa26-CreER- control littermates. Six days later total splenocytes were isolated, pooled from 2-3 mice and enriched for lineage (CD3/CD19)-negative cells by magnetic selection. PDC (CD11b- B220+ CD11clow Bst2+) were isolated using BD FACSAria II flow sorter directly into Trizol reagent.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was extracted using Trizol reagent (Invitrogen).
Sample_label_ch1	biotin
Sample_label_protocol_ch1	The aRNA was amplified without labeling in the first round, and then labeled with Biotin-16-UTP and Biotin-11-CTP in the second round. Labeled aRNA was fragmented in RNA Fragmentation Buffer (QIAGEN) prior to hybridization.
Sample_hyb_protocol	Labeled RNA samples (10 ug/array) were hybridized at the HICCC Genomics Facility of the Columbia University according to the manufacturer's instructions.
Sample_scan_protocol	Array scanning and raw data processing were done using GCOS 1.4 software (Affymetrix).
Sample_data_processing	MAS5.0
Sample_platform_id	GPL1261
Sample_contact_name	Boris,,Reizis
Sample_contact_email	bvr2101@columbia.edu
Sample_contact_department	Microbiology and Immunology
Sample_contact_institute	Columbia University Medical Center
Sample_contact_address	701 W 168th St.
Sample_contact_city	New York
Sample_contact_state	NY
Sample_contact_zip/postal_code	10032
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM609nnn/GSM609041/suppl/GSM609041.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM609nnn/GSM609041/suppl/GSM609041.CHP.gz
Sample_series_id	GSE24726
Sample_series_id	GSE24785
Sample_data_row_count	45101

GSM609043

GPL1261

PDC_control_day6_rep2

Ex vivo sorted plasmacytoid dendritic cells (PDC)

strain: C57BL/6 genotype/varation: control time point: day6 tissue: spleen cell type: PDC agent: tamoxifen

Sample_geo_accession	GSM609043
Sample_status	Public on Dec 21 2010
Sample_submission_date	Oct 15 2010
Sample_last_update_date	Dec 21 2010
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Inducible deletion of E2-2 (Tcf4) has been performed by administering tamoxifen to conditional E2-2flox/flox Rosa26-CreER+ mice or to E2-2flox/flox Rosa26-CreER- control littermates. Six days later total splenocytes were isolated, pooled from 2-3 mice and enriched for lineage (CD3/CD19)-negative cells by magnetic selection. PDC (CD11b- B220+ CD11clow Bst2+) were isolated using BD FACSAria II flow sorter directly into Trizol reagent.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was extracted using Trizol reagent (Invitrogen).
Sample_label_ch1	biotin
Sample_label_protocol_ch1	The aRNA was amplified without labeling in the first round, and then labeled with Biotin-16-UTP and Biotin-11-CTP in the second round. Labeled aRNA was fragmented in RNA Fragmentation Buffer (QIAGEN) prior to hybridization.
Sample_hyb_protocol	Labeled RNA samples (10 ug/array) were hybridized at the HICCC Genomics Facility of the Columbia University according to the manufacturer's instructions.
Sample_scan_protocol	Array scanning and raw data processing were done using GCOS 1.4 software (Affymetrix).
Sample_data_processing	MAS5.0
Sample_platform_id	GPL1261
Sample_contact_name	Boris,,Reizis
Sample_contact_email	bvr2101@columbia.edu
Sample_contact_department	Microbiology and Immunology
Sample_contact_institute	Columbia University Medical Center
Sample_contact_address	701 W 168th St.
Sample_contact_city	New York
Sample_contact_state	NY
Sample_contact_zip/postal_code	10032
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM609nnn/GSM609043/suppl/GSM609043.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM609nnn/GSM609043/suppl/GSM609043.CHP.gz
Sample_series_id	GSE24726
Sample_series_id	GSE24785
Sample_data_row_count	45101

GSM609044

GPL1261

PDC_null_day4_rep1

Ex vivo sorted plasmacytoid dendritic cells (PDC)

strain: C57BL/6 genotype/varation: E2-2-deficient (null) time point: day4 tissue: spleen cell type: PDC agent: tamoxifen

Sample_geo_accession	GSM609044
Sample_status	Public on Dec 21 2010
Sample_submission_date	Oct 15 2010
Sample_last_update_date	Dec 21 2010
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Inducible deletion of E2-2 (Tcf4) has been performed by administering tamoxifen to conditional E2-2flox/flox Rosa26-CreER+ mice or to E2-2flox/flox Rosa26-CreER- control littermates. Six days later total splenocytes were isolated, pooled from 2-3 mice and enriched for lineage (CD3/CD19)-negative cells by magnetic selection. PDC (CD11b- B220+ CD11clow Bst2+) were isolated using BD FACSAria II flow sorter directly into Trizol reagent.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was extracted using Trizol reagent (Invitrogen).
Sample_label_ch1	biotin
Sample_label_protocol_ch1	The aRNA was amplified without labeling in the first round, and then labeled with Biotin-16-UTP and Biotin-11-CTP in the second round. Labeled aRNA was fragmented in RNA Fragmentation Buffer (QIAGEN) prior to hybridization.
Sample_hyb_protocol	Labeled RNA samples (10 ug/array) were hybridized at the HICCC Genomics Facility of the Columbia University according to the manufacturer's instructions.
Sample_scan_protocol	Array scanning and raw data processing were done using GCOS 1.4 software (Affymetrix).
Sample_data_processing	MAS5.0
Sample_platform_id	GPL1261
Sample_contact_name	Boris,,Reizis
Sample_contact_email	bvr2101@columbia.edu
Sample_contact_department	Microbiology and Immunology
Sample_contact_institute	Columbia University Medical Center
Sample_contact_address	701 W 168th St.
Sample_contact_city	New York
Sample_contact_state	NY
Sample_contact_zip/postal_code	10032
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM609nnn/GSM609044/suppl/GSM609044.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM609nnn/GSM609044/suppl/GSM609044.CHP.gz
Sample_series_id	GSE24726
Sample_series_id	GSE24785
Sample_data_row_count	45101

GSM609045

GPL1261

PDC_null_day4_rep2

Ex vivo sorted plasmacytoid dendritic cells (PDC)

strain: C57BL/6 genotype/varation: E2-2-deficient (null) time point: day4 tissue: spleen cell type: PDC agent: tamoxifen

Sample_geo_accession	GSM609045
Sample_status	Public on Dec 21 2010
Sample_submission_date	Oct 15 2010
Sample_last_update_date	Dec 21 2010
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Inducible deletion of E2-2 (Tcf4) has been performed by administering tamoxifen to conditional E2-2flox/flox Rosa26-CreER+ mice or to E2-2flox/flox Rosa26-CreER- control littermates. Six days later total splenocytes were isolated, pooled from 2-3 mice and enriched for lineage (CD3/CD19)-negative cells by magnetic selection. PDC (CD11b- B220+ CD11clow Bst2+) were isolated using BD FACSAria II flow sorter directly into Trizol reagent.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was extracted using Trizol reagent (Invitrogen).
Sample_label_ch1	biotin
Sample_label_protocol_ch1	The aRNA was amplified without labeling in the first round, and then labeled with Biotin-16-UTP and Biotin-11-CTP in the second round. Labeled aRNA was fragmented in RNA Fragmentation Buffer (QIAGEN) prior to hybridization.
Sample_hyb_protocol	Labeled RNA samples (10 ug/array) were hybridized at the HICCC Genomics Facility of the Columbia University according to the manufacturer's instructions.
Sample_scan_protocol	Array scanning and raw data processing were done using GCOS 1.4 software (Affymetrix).
Sample_data_processing	MAS5.0
Sample_platform_id	GPL1261
Sample_contact_name	Boris,,Reizis
Sample_contact_email	bvr2101@columbia.edu
Sample_contact_department	Microbiology and Immunology
Sample_contact_institute	Columbia University Medical Center
Sample_contact_address	701 W 168th St.
Sample_contact_city	New York
Sample_contact_state	NY
Sample_contact_zip/postal_code	10032
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM609nnn/GSM609045/suppl/GSM609045.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM609nnn/GSM609045/suppl/GSM609045.CHP.gz
Sample_series_id	GSE24726
Sample_series_id	GSE24785
Sample_data_row_count	45101

GSM609063

GPL1261

PDC_null_day6_rep1

Ex vivo sorted plasmacytoid dendritic cells (PDC)

strain: C57BL/6 genotype/varation: E2-2-deficient (null) time point: day6 tissue: spleen cell type: PDC agent: tamoxifen

Sample_geo_accession	GSM609063
Sample_status	Public on Dec 21 2010
Sample_submission_date	Oct 15 2010
Sample_last_update_date	Dec 21 2010
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Inducible deletion of E2-2 (Tcf4) has been performed by administering tamoxifen to conditional E2-2flox/flox Rosa26-CreER+ mice or to E2-2flox/flox Rosa26-CreER- control littermates. Six days later total splenocytes were isolated, pooled from 2-3 mice and enriched for lineage (CD3/CD19)-negative cells by magnetic selection. PDC (CD11b- B220+ CD11clow Bst2+) were isolated using BD FACSAria II flow sorter directly into Trizol reagent.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was extracted using Trizol reagent (Invitrogen).
Sample_label_ch1	biotin
Sample_label_protocol_ch1	The aRNA was amplified without labeling in the first round, and then labeled with Biotin-16-UTP and Biotin-11-CTP in the second round. Labeled aRNA was fragmented in RNA Fragmentation Buffer (QIAGEN) prior to hybridization.
Sample_hyb_protocol	Labeled RNA samples (10 ug/array) were hybridized at the HICCC Genomics Facility of the Columbia University according to the manufacturer's instructions.
Sample_scan_protocol	Array scanning and raw data processing were done using GCOS 1.4 software (Affymetrix).
Sample_data_processing	MAS5.0
Sample_platform_id	GPL1261
Sample_contact_name	Boris,,Reizis
Sample_contact_email	bvr2101@columbia.edu
Sample_contact_department	Microbiology and Immunology
Sample_contact_institute	Columbia University Medical Center
Sample_contact_address	701 W 168th St.
Sample_contact_city	New York
Sample_contact_state	NY
Sample_contact_zip/postal_code	10032
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM609nnn/GSM609063/suppl/GSM609063.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM609nnn/GSM609063/suppl/GSM609063.CHP.gz
Sample_series_id	GSE24726
Sample_series_id	GSE24785
Sample_data_row_count	45101

GSM609075

GPL1261

PDC_null_day6_rep2

Ex vivo sorted plasmacytoid dendritic cells (PDC)

strain: C57BL/6 genotype/varation: E2-2-deficient (null) time point: day6 tissue: spleen cell type: PDC agent: tamoxifen

Sample_geo_accession	GSM609075
Sample_status	Public on Dec 21 2010
Sample_submission_date	Oct 15 2010
Sample_last_update_date	Dec 21 2010
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	Inducible deletion of E2-2 (Tcf4) has been performed by administering tamoxifen to conditional E2-2flox/flox Rosa26-CreER+ mice or to E2-2flox/flox Rosa26-CreER- control littermates. Six days later total splenocytes were isolated, pooled from 2-3 mice and enriched for lineage (CD3/CD19)-negative cells by magnetic selection. PDC (CD11b- B220+ CD11clow Bst2+) were isolated using BD FACSAria II flow sorter directly into Trizol reagent.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was extracted using Trizol reagent (Invitrogen).
Sample_label_ch1	biotin
Sample_label_protocol_ch1	The aRNA was amplified without labeling in the first round, and then labeled with Biotin-16-UTP and Biotin-11-CTP in the second round. Labeled aRNA was fragmented in RNA Fragmentation Buffer (QIAGEN) prior to hybridization.
Sample_hyb_protocol	Labeled RNA samples (10 ug/array) were hybridized at the HICCC Genomics Facility of the Columbia University according to the manufacturer's instructions.
Sample_scan_protocol	Array scanning and raw data processing were done using GCOS 1.4 software (Affymetrix).
Sample_data_processing	MAS5.0
Sample_platform_id	GPL1261
Sample_contact_name	Boris,,Reizis
Sample_contact_email	bvr2101@columbia.edu
Sample_contact_department	Microbiology and Immunology
Sample_contact_institute	Columbia University Medical Center
Sample_contact_address	701 W 168th St.
Sample_contact_city	New York
Sample_contact_state	NY
Sample_contact_zip/postal_code	10032
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM609nnn/GSM609075/suppl/GSM609075.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM609nnn/GSM609075/suppl/GSM609075.CHP.gz
Sample_series_id	GSE24726
Sample_series_id	GSE24785
Sample_data_row_count	45101

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