Search results for the GEO ID: GSE24783 |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM610399 | GPL570 |
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HSC-3 cells (control)
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Human oral squamous carcinoma cell line HSC-3
|
cell line: HSC-3
treatment: control
|
HSC-3 cells, a human oral squamous carcinoma cell line, were cultured at 37°C. Non-treated cells were served as control.
|
Sample_geo_accession | GSM610399
| Sample_status | Public on Oct 20 2010
| Sample_submission_date | Oct 18 2010
| Sample_last_update_date | Oct 19 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted from the cells using an RNeasy Total RNA Extraction Kit (Qiagen K. K., Tokyo, Japan). Then, RNA samples were treated with RNase-free DNase.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | 5 μg of total RNA was used to synthesize double-strand cDNA with a GeneChip® Expression 3’-Amplification Reagents One-Cycle cDNA Synthesis Kit (Affymetrix). Biotin-labeled cRNA was then synthesized from the cDNA using GeneChip® Expression 3’-Amplification Reagents for IVT Labeling (Affymetrix).
| Sample_hyb_protocol | After fragmentation, the biotinylated cRNA was hybridized to arrays at 45°C for 16 h.
| Sample_scan_protocol | The arrays were washed, stained with streptavidin-phycoerythrin and scanned with a probe array scanner (Affymetrix).
| Sample_data_processing | Standard Affymetrix protocol
| Sample_platform_id | GPL570
| Sample_contact_name | Yoshiaki,,Tabuchi
| Sample_contact_email | ytabu@cts.u-toyama.ac.jp
| Sample_contact_laboratory | Division of Molecular Genetics Research
| Sample_contact_department | Life Science Research Center
| Sample_contact_institute | University of Toyama
| Sample_contact_address | 2630 Sugitani
| Sample_contact_city | Toyama
| Sample_contact_state | Toyama
| Sample_contact_zip/postal_code | 930-0194
| Sample_contact_country | Japan
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM610nnn/GSM610399/suppl/GSM610399_1_CT.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM610nnn/GSM610399/suppl/GSM610399_1_CT.CHP.gz
| Sample_series_id | GSE24783
| Sample_data_row_count | 54675
| |
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GSM610400 | GPL570 |
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HSC-3 cells treated with heat stress at 42°C for 0 h
|
Human oral squamous carcinoma cell line HSC-3
|
cell line: HSC-3
treatment: 42 C
time: 0h
|
HSC-3 cells, a human oral squamous carcinoma cell line, were cultured at 37°C. The cells were treated with heat stress 42°C for 90 min and followed by incubation for 0 h at 37°C. Non-treated cells were served as control.
|
Sample_geo_accession | GSM610400
| Sample_status | Public on Oct 20 2010
| Sample_submission_date | Oct 18 2010
| Sample_last_update_date | Oct 19 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted from the cells using an RNeasy Total RNA Extraction Kit (Qiagen K. K., Tokyo, Japan). Then, RNA samples were treated with RNase-free DNase.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | 5 μg of total RNA was used to synthesize double-strand cDNA with a GeneChip® Expression 3’-Amplification Reagents One-Cycle cDNA Synthesis Kit (Affymetrix). Biotin-labeled cRNA was then synthesized from the cDNA using GeneChip® Expression 3’-Amplification Reagents for IVT Labeling (Affymetrix).
| Sample_hyb_protocol | After fragmentation, the biotinylated cRNA was hybridized to arrays at 45°C for 16 h.
| Sample_scan_protocol | The arrays were washed, stained with streptavidin-phycoerythrin and scanned with a probe array scanner (Affymetrix).
| Sample_data_processing | Standard Affymetrix protocol
| Sample_platform_id | GPL570
| Sample_contact_name | Yoshiaki,,Tabuchi
| Sample_contact_email | ytabu@cts.u-toyama.ac.jp
| Sample_contact_laboratory | Division of Molecular Genetics Research
| Sample_contact_department | Life Science Research Center
| Sample_contact_institute | University of Toyama
| Sample_contact_address | 2630 Sugitani
| Sample_contact_city | Toyama
| Sample_contact_state | Toyama
| Sample_contact_zip/postal_code | 930-0194
| Sample_contact_country | Japan
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM610nnn/GSM610400/suppl/GSM610400_5_42c_0h.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM610nnn/GSM610400/suppl/GSM610400_5_42c_0h.CHP.gz
| Sample_series_id | GSE24783
| Sample_data_row_count | 54675
| |
|
GSM610401 | GPL570 |
|
HSC-3 cells treated with heat stress at 42°C for 6 h
|
Human oral squamous carcinoma cell line HSC-3
|
cell line: HSC-3
treatment: 42 C
time: 6h
|
HSC-3 cells, a human oral squamous carcinoma cell line, were cultured at 37°C. The cells were treated with heat stress 42°C for 90 min and followed by incubation for 6 h at 37°C. Non-treated cells were served as control.
|
Sample_geo_accession | GSM610401
| Sample_status | Public on Oct 20 2010
| Sample_submission_date | Oct 18 2010
| Sample_last_update_date | Oct 19 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted from the cells using an RNeasy Total RNA Extraction Kit (Qiagen K. K., Tokyo, Japan). Then, RNA samples were treated with RNase-free DNase.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | 5 μg of total RNA was used to synthesize double-strand cDNA with a GeneChip® Expression 3’-Amplification Reagents One-Cycle cDNA Synthesis Kit (Affymetrix). Biotin-labeled cRNA was then synthesized from the cDNA using GeneChip® Expression 3’-Amplification Reagents for IVT Labeling (Affymetrix).
| Sample_hyb_protocol | After fragmentation, the biotinylated cRNA was hybridized to arrays at 45°C for 16 h.
| Sample_scan_protocol | The arrays were washed, stained with streptavidin-phycoerythrin and scanned with a probe array scanner (Affymetrix).
| Sample_data_processing | Standard Affymetrix protocol
| Sample_platform_id | GPL570
| Sample_contact_name | Yoshiaki,,Tabuchi
| Sample_contact_email | ytabu@cts.u-toyama.ac.jp
| Sample_contact_laboratory | Division of Molecular Genetics Research
| Sample_contact_department | Life Science Research Center
| Sample_contact_institute | University of Toyama
| Sample_contact_address | 2630 Sugitani
| Sample_contact_city | Toyama
| Sample_contact_state | Toyama
| Sample_contact_zip/postal_code | 930-0194
| Sample_contact_country | Japan
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM610nnn/GSM610401/suppl/GSM610401_6_42c_6h.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM610nnn/GSM610401/suppl/GSM610401_6_42c_6h.CHP.gz
| Sample_series_id | GSE24783
| Sample_data_row_count | 54675
| |
|
GSM610402 | GPL570 |
|
HSC-3 cells treated with heat stress at 42°C for 12 h
|
Human oral squamous carcinoma cell line HSC-3
|
cell line: HSC-3
treatment: 42 C
time: 12h
|
HSC-3 cells, a human oral squamous carcinoma cell line, were cultured at 37°C. The cells were treated with heat stress 42°C for 90 min and followed by incubation for 12 h at 37°C. Non-treated cells were served as control.
|
Sample_geo_accession | GSM610402
| Sample_status | Public on Oct 20 2010
| Sample_submission_date | Oct 18 2010
| Sample_last_update_date | Oct 19 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted from the cells using an RNeasy Total RNA Extraction Kit (Qiagen K. K., Tokyo, Japan). Then, RNA samples were treated with RNase-free DNase.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | 5 μg of total RNA was used to synthesize double-strand cDNA with a GeneChip® Expression 3’-Amplification Reagents One-Cycle cDNA Synthesis Kit (Affymetrix). Biotin-labeled cRNA was then synthesized from the cDNA using GeneChip® Expression 3’-Amplification Reagents for IVT Labeling (Affymetrix).
| Sample_hyb_protocol | After fragmentation, the biotinylated cRNA was hybridized to arrays at 45°C for 16 h.
| Sample_scan_protocol | The arrays were washed, stained with streptavidin-phycoerythrin and scanned with a probe array scanner (Affymetrix).
| Sample_data_processing | Standard Affymetrix protocol
| Sample_platform_id | GPL570
| Sample_contact_name | Yoshiaki,,Tabuchi
| Sample_contact_email | ytabu@cts.u-toyama.ac.jp
| Sample_contact_laboratory | Division of Molecular Genetics Research
| Sample_contact_department | Life Science Research Center
| Sample_contact_institute | University of Toyama
| Sample_contact_address | 2630 Sugitani
| Sample_contact_city | Toyama
| Sample_contact_state | Toyama
| Sample_contact_zip/postal_code | 930-0194
| Sample_contact_country | Japan
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM610nnn/GSM610402/suppl/GSM610402_7_42c_12h.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM610nnn/GSM610402/suppl/GSM610402_7_42c_12h.CHP.gz
| Sample_series_id | GSE24783
| Sample_data_row_count | 54675
| |
|
GSM610403 | GPL570 |
|
HSC-3 cells treated with heat stress at 44°C for 0 h
|
Human oral squamous carcinoma cell line HSC-3
|
cell line: HSC-3
treatment: 44 C
time: 0h
|
HSC-3 cells, a human oral squamous carcinoma cell line, were cultured at 37°C. The cells were treated with heat stress 44°C for 90 min and followed by incubation for 0 h at 37°C. Non-treated cells were served as control.
|
Sample_geo_accession | GSM610403
| Sample_status | Public on Oct 20 2010
| Sample_submission_date | Oct 18 2010
| Sample_last_update_date | Oct 19 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted from the cells using an RNeasy Total RNA Extraction Kit (Qiagen K. K., Tokyo, Japan). Then, RNA samples were treated with RNase-free DNase.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | 5 μg of total RNA was used to synthesize double-strand cDNA with a GeneChip® Expression 3’-Amplification Reagents One-Cycle cDNA Synthesis Kit (Affymetrix). Biotin-labeled cRNA was then synthesized from the cDNA using GeneChip® Expression 3’-Amplification Reagents for IVT Labeling (Affymetrix).
| Sample_hyb_protocol | After fragmentation, the biotinylated cRNA was hybridized to arrays at 45°C for 16 h.
| Sample_scan_protocol | The arrays were washed, stained with streptavidin-phycoerythrin and scanned with a probe array scanner (Affymetrix).
| Sample_data_processing | Standard Affymetrix protocol
| Sample_platform_id | GPL570
| Sample_contact_name | Yoshiaki,,Tabuchi
| Sample_contact_email | ytabu@cts.u-toyama.ac.jp
| Sample_contact_laboratory | Division of Molecular Genetics Research
| Sample_contact_department | Life Science Research Center
| Sample_contact_institute | University of Toyama
| Sample_contact_address | 2630 Sugitani
| Sample_contact_city | Toyama
| Sample_contact_state | Toyama
| Sample_contact_zip/postal_code | 930-0194
| Sample_contact_country | Japan
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM610nnn/GSM610403/suppl/GSM610403_2_44c_0h.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM610nnn/GSM610403/suppl/GSM610403_2_44c_0h.CHP.gz
| Sample_series_id | GSE24783
| Sample_data_row_count | 54675
| |
|
GSM610404 | GPL570 |
|
HSC-3 cells treated with heat stress at 44°C for 6 h
|
Human oral squamous carcinoma cell line HSC-3
|
cell line: HSC-3
treatment: 44 C
time: 6h
|
HSC-3 cells, a human oral squamous carcinoma cell line, were cultured at 37°C. The cells were treated with heat stress 44°C for 90 min and followed by incubation for 6 h at 37°C. Non-treated cells were served as control.
|
Sample_geo_accession | GSM610404
| Sample_status | Public on Oct 20 2010
| Sample_submission_date | Oct 18 2010
| Sample_last_update_date | Oct 19 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted from the cells using an RNeasy Total RNA Extraction Kit (Qiagen K. K., Tokyo, Japan). Then, RNA samples were treated with RNase-free DNase.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | 5 μg of total RNA was used to synthesize double-strand cDNA with a GeneChip® Expression 3’-Amplification Reagents One-Cycle cDNA Synthesis Kit (Affymetrix). Biotin-labeled cRNA was then synthesized from the cDNA using GeneChip® Expression 3’-Amplification Reagents for IVT Labeling (Affymetrix).
| Sample_hyb_protocol | After fragmentation, the biotinylated cRNA was hybridized to arrays at 45°C for 16 h.
| Sample_scan_protocol | The arrays were washed, stained with streptavidin-phycoerythrin and scanned with a probe array scanner (Affymetrix).
| Sample_data_processing | Standard Affymetrix protocol
| Sample_platform_id | GPL570
| Sample_contact_name | Yoshiaki,,Tabuchi
| Sample_contact_email | ytabu@cts.u-toyama.ac.jp
| Sample_contact_laboratory | Division of Molecular Genetics Research
| Sample_contact_department | Life Science Research Center
| Sample_contact_institute | University of Toyama
| Sample_contact_address | 2630 Sugitani
| Sample_contact_city | Toyama
| Sample_contact_state | Toyama
| Sample_contact_zip/postal_code | 930-0194
| Sample_contact_country | Japan
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM610nnn/GSM610404/suppl/GSM610404_3_44c_6h.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM610nnn/GSM610404/suppl/GSM610404_3_44c_6h.CHP.gz
| Sample_series_id | GSE24783
| Sample_data_row_count | 54675
| |
|
GSM610405 | GPL570 |
|
HSC-3 cells treated with heat stress at 44°C for 12 h
|
Human oral squamous carcinoma cell line HSC-3
|
cell line: HSC-3
treatment: 44 C
time: 12h
|
HSC-3 cells, a human oral squamous carcinoma cell line, were cultured at 37°C. The cells were treated with heat stress 44°C for 90 min and followed by incubation for 12 h at 37°C. Non-treated cells were served as control.
|
Sample_geo_accession | GSM610405
| Sample_status | Public on Oct 20 2010
| Sample_submission_date | Oct 18 2010
| Sample_last_update_date | Oct 19 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted from the cells using an RNeasy Total RNA Extraction Kit (Qiagen K. K., Tokyo, Japan). Then, RNA samples were treated with RNase-free DNase.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | 5 μg of total RNA was used to synthesize double-strand cDNA with a GeneChip® Expression 3’-Amplification Reagents One-Cycle cDNA Synthesis Kit (Affymetrix). Biotin-labeled cRNA was then synthesized from the cDNA using GeneChip® Expression 3’-Amplification Reagents for IVT Labeling (Affymetrix).
| Sample_hyb_protocol | After fragmentation, the biotinylated cRNA was hybridized to arrays at 45°C for 16 h.
| Sample_scan_protocol | The arrays were washed, stained with streptavidin-phycoerythrin and scanned with a probe array scanner (Affymetrix).
| Sample_data_processing | Standard Affymetrix protocol
| Sample_platform_id | GPL570
| Sample_contact_name | Yoshiaki,,Tabuchi
| Sample_contact_email | ytabu@cts.u-toyama.ac.jp
| Sample_contact_laboratory | Division of Molecular Genetics Research
| Sample_contact_department | Life Science Research Center
| Sample_contact_institute | University of Toyama
| Sample_contact_address | 2630 Sugitani
| Sample_contact_city | Toyama
| Sample_contact_state | Toyama
| Sample_contact_zip/postal_code | 930-0194
| Sample_contact_country | Japan
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM610nnn/GSM610405/suppl/GSM610405_4_44c_12h.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM610nnn/GSM610405/suppl/GSM610405_4_44c_12h.CHP.gz
| Sample_series_id | GSE24783
| Sample_data_row_count | 54675
| |
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