Search results for the GEO ID: GSE24789  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM610470 | GPL1261 |
|
MOSE Early Cells, biological replicate 1
|
mouse ovarian surface epithelial cells in culture
|
cell type: mouse ovarian surface epithelial (MOSE) cell
malignant potential: non-malignant
|
1_p2.13.CEL
Gene Expression of MOSE cells at different transformation stages
|
| Sample_geo_accession | GSM610470
| | Sample_status | Public on Mar 03 2011
| | Sample_submission_date | Oct 19 2010
| | Sample_last_update_date | Mar 03 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_growth_protocol_ch1 | MOSE cell lines were routinely maintained in DMEM high glucose medium supplemented with 4% fetal bovine serum, 100 mg/ml each of penicillin and streptomycin, 5 mg/ml insulin, 5 mg/ml transferrin, and 5 ng/ml sodium selenite.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA samples for early (passage 13, 14, and 15), intermediate (63, 71, and 73), and late (136, 142, and 143) passages were isolated using the RNeasy Kit according to the manufacturers instructions and treated with ribonuclease-free deoxyribonuclease I. RNA samples were assayed on the Agilent 2100 BioAnalyzer for qualitative assessment and quantification.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA.
| | Sample_hyb_protocol | The RNA samples were processed using Affymetrix's GeneChip WT cDNA Synthesis and Amplification Kit (900672). Hybridization was done using Affymetrix Hybridization, Wash, and Stain Kit (part # 900720). Arrays were hybed for 16 hr at 45C. Samples were stained and washed using the Genechip Fluidics 450 station
| | Sample_scan_protocol | GeneChips were scanned using the GCS 3000 HR scanner.
| | Sample_data_processing | We utilized MicroArray Suite 5.0 (Affymetrix, Santa Clara, CA) to process raw microarray data. Data values were globally scaled to an average target intensity to allow inter-GeneChip comparisons.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Eva,M,Schmelz
| | Sample_contact_email | eschmelz@vt.edu
| | Sample_contact_laboratory | Schmelz
| | Sample_contact_department | HNFE
| | Sample_contact_institute | Virginia Tech
| | Sample_contact_address | Virginia Tech
| | Sample_contact_city | Blacksburg
| | Sample_contact_state | VA
| | Sample_contact_zip/postal_code | 24060
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM610nnn/GSM610470/suppl/GSM610470.CEL.gz
| | Sample_series_id | GSE24789
| | Sample_data_row_count | 45101
| | |
|
GSM610471 | GPL1261 |
|
MOSE Early Cells, biological replicate 2
|
mouse ovarian surface epithelial cells in culture
|
cell type: mouse ovarian surface epithelial (MOSE) cell
malignant potential: non-malignant
|
2_p2.14.CEL
Gene Expression of MOSE cells at different transformation stages
|
| Sample_geo_accession | GSM610471
| | Sample_status | Public on Mar 03 2011
| | Sample_submission_date | Oct 19 2010
| | Sample_last_update_date | Mar 03 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_growth_protocol_ch1 | MOSE cell lines were routinely maintained in DMEM high glucose medium supplemented with 4% fetal bovine serum, 100 mg/ml each of penicillin and streptomycin, 5 mg/ml insulin, 5 mg/ml transferrin, and 5 ng/ml sodium selenite.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA samples for early (passage 13, 14, and 15), intermediate (63, 71, and 73), and late (136, 142, and 143) passages were isolated using the RNeasy Kit according to the manufacturers instructions and treated with ribonuclease-free deoxyribonuclease I. RNA samples were assayed on the Agilent 2100 BioAnalyzer for qualitative assessment and quantification.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA.
| | Sample_hyb_protocol | The RNA samples were processed using Affymetrix's GeneChip WT cDNA Synthesis and Amplification Kit (900672). Hybridization was done using Affymetrix Hybridization, Wash, and Stain Kit (part # 900720). Arrays were hybed for 16 hr at 45C. Samples were stained and washed using the Genechip Fluidics 450 station
| | Sample_scan_protocol | GeneChips were scanned using the GCS 3000 HR scanner.
| | Sample_data_processing | We utilized MicroArray Suite 5.0 (Affymetrix, Santa Clara, CA) to process raw microarray data. Data values were globally scaled to an average target intensity to allow inter-GeneChip comparisons.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Eva,M,Schmelz
| | Sample_contact_email | eschmelz@vt.edu
| | Sample_contact_laboratory | Schmelz
| | Sample_contact_department | HNFE
| | Sample_contact_institute | Virginia Tech
| | Sample_contact_address | Virginia Tech
| | Sample_contact_city | Blacksburg
| | Sample_contact_state | VA
| | Sample_contact_zip/postal_code | 24060
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM610nnn/GSM610471/suppl/GSM610471.CEL.gz
| | Sample_series_id | GSE24789
| | Sample_data_row_count | 45101
| | |
|
GSM610472 | GPL1261 |
|
MOSE Early Cells, biological replicate 3
|
mouse ovarian surface epithelial cells in culture
|
cell type: mouse ovarian surface epithelial (MOSE) cell
malignant potential: non-malignant
|
3_p2.15.CEL
Gene Expression of MOSE cells at different transformation stages
|
| Sample_geo_accession | GSM610472
| | Sample_status | Public on Mar 03 2011
| | Sample_submission_date | Oct 19 2010
| | Sample_last_update_date | Mar 03 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_growth_protocol_ch1 | MOSE cell lines were routinely maintained in DMEM high glucose medium supplemented with 4% fetal bovine serum, 100 mg/ml each of penicillin and streptomycin, 5 mg/ml insulin, 5 mg/ml transferrin, and 5 ng/ml sodium selenite.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA samples for early (passage 13, 14, and 15), intermediate (63, 71, and 73), and late (136, 142, and 143) passages were isolated using the RNeasy Kit according to the manufacturers instructions and treated with ribonuclease-free deoxyribonuclease I. RNA samples were assayed on the Agilent 2100 BioAnalyzer for qualitative assessment and quantification.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA.
| | Sample_hyb_protocol | The RNA samples were processed using Affymetrix's GeneChip WT cDNA Synthesis and Amplification Kit (900672). Hybridization was done using Affymetrix Hybridization, Wash, and Stain Kit (part # 900720). Arrays were hybed for 16 hr at 45C. Samples were stained and washed using the Genechip Fluidics 450 station
| | Sample_scan_protocol | GeneChips were scanned using the GCS 3000 HR scanner.
| | Sample_data_processing | We utilized MicroArray Suite 5.0 (Affymetrix, Santa Clara, CA) to process raw microarray data. Data values were globally scaled to an average target intensity to allow inter-GeneChip comparisons.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Eva,M,Schmelz
| | Sample_contact_email | eschmelz@vt.edu
| | Sample_contact_laboratory | Schmelz
| | Sample_contact_department | HNFE
| | Sample_contact_institute | Virginia Tech
| | Sample_contact_address | Virginia Tech
| | Sample_contact_city | Blacksburg
| | Sample_contact_state | VA
| | Sample_contact_zip/postal_code | 24060
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM610nnn/GSM610472/suppl/GSM610472.CEL.gz
| | Sample_series_id | GSE24789
| | Sample_data_row_count | 45101
| | |
|
GSM610473 | GPL1261 |
|
MOSE Intermediate Cells, biological replicate 1
|
mouse ovarian surface epithelial cells in culture
|
cell type: mouse ovarian surface epithelial (MOSE) cell
malignant potential: intermediate
|
4_p2.63.CEL
Gene Expression of MOSE cells at different transformation stages
|
| Sample_geo_accession | GSM610473
| | Sample_status | Public on Mar 03 2011
| | Sample_submission_date | Oct 19 2010
| | Sample_last_update_date | Mar 03 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_growth_protocol_ch1 | MOSE cell lines were routinely maintained in DMEM high glucose medium supplemented with 4% fetal bovine serum, 100 mg/ml each of penicillin and streptomycin, 5 mg/ml insulin, 5 mg/ml transferrin, and 5 ng/ml sodium selenite.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA samples for early (passage 13, 14, and 15), intermediate (63, 71, and 73), and late (136, 142, and 143) passages were isolated using the RNeasy Kit according to the manufacturers instructions and treated with ribonuclease-free deoxyribonuclease I. RNA samples were assayed on the Agilent 2100 BioAnalyzer for qualitative assessment and quantification.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA.
| | Sample_hyb_protocol | The RNA samples were processed using Affymetrix's GeneChip WT cDNA Synthesis and Amplification Kit (900672). Hybridization was done using Affymetrix Hybridization, Wash, and Stain Kit (part # 900720). Arrays were hybed for 16 hr at 45C. Samples were stained and washed using the Genechip Fluidics 450 station
| | Sample_scan_protocol | GeneChips were scanned using the GCS 3000 HR scanner.
| | Sample_data_processing | We utilized MicroArray Suite 5.0 (Affymetrix, Santa Clara, CA) to process raw microarray data. Data values were globally scaled to an average target intensity to allow inter-GeneChip comparisons.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Eva,M,Schmelz
| | Sample_contact_email | eschmelz@vt.edu
| | Sample_contact_laboratory | Schmelz
| | Sample_contact_department | HNFE
| | Sample_contact_institute | Virginia Tech
| | Sample_contact_address | Virginia Tech
| | Sample_contact_city | Blacksburg
| | Sample_contact_state | VA
| | Sample_contact_zip/postal_code | 24060
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM610nnn/GSM610473/suppl/GSM610473.CEL.gz
| | Sample_series_id | GSE24789
| | Sample_data_row_count | 45101
| | |
|
GSM610474 | GPL1261 |
|
MOSE Intermediate Cells, biological replicate 2
|
mouse ovarian surface epithelial cells in culture
|
cell type: mouse ovarian surface epithelial (MOSE) cell
malignant potential: intermediate
|
5_p2.71.CEL
Gene Expression of MOSE cells at different transformation stages
|
| Sample_geo_accession | GSM610474
| | Sample_status | Public on Mar 03 2011
| | Sample_submission_date | Oct 19 2010
| | Sample_last_update_date | Mar 03 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_growth_protocol_ch1 | MOSE cell lines were routinely maintained in DMEM high glucose medium supplemented with 4% fetal bovine serum, 100 mg/ml each of penicillin and streptomycin, 5 mg/ml insulin, 5 mg/ml transferrin, and 5 ng/ml sodium selenite.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA samples for early (passage 13, 14, and 15), intermediate (63, 71, and 73), and late (136, 142, and 143) passages were isolated using the RNeasy Kit according to the manufacturers instructions and treated with ribonuclease-free deoxyribonuclease I. RNA samples were assayed on the Agilent 2100 BioAnalyzer for qualitative assessment and quantification.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA.
| | Sample_hyb_protocol | The RNA samples were processed using Affymetrix's GeneChip WT cDNA Synthesis and Amplification Kit (900672). Hybridization was done using Affymetrix Hybridization, Wash, and Stain Kit (part # 900720). Arrays were hybed for 16 hr at 45C. Samples were stained and washed using the Genechip Fluidics 450 station
| | Sample_scan_protocol | GeneChips were scanned using the GCS 3000 HR scanner.
| | Sample_data_processing | We utilized MicroArray Suite 5.0 (Affymetrix, Santa Clara, CA) to process raw microarray data. Data values were globally scaled to an average target intensity to allow inter-GeneChip comparisons.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Eva,M,Schmelz
| | Sample_contact_email | eschmelz@vt.edu
| | Sample_contact_laboratory | Schmelz
| | Sample_contact_department | HNFE
| | Sample_contact_institute | Virginia Tech
| | Sample_contact_address | Virginia Tech
| | Sample_contact_city | Blacksburg
| | Sample_contact_state | VA
| | Sample_contact_zip/postal_code | 24060
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM610nnn/GSM610474/suppl/GSM610474.CEL.gz
| | Sample_series_id | GSE24789
| | Sample_data_row_count | 45101
| | |
|
GSM610475 | GPL1261 |
|
MOSE Intermediate Cells, biological replicate 3
|
mouse ovarian surface epithelial cells in culture
|
cell type: mouse ovarian surface epithelial (MOSE) cell
malignant potential: intermediate
|
6_p2.73.CEL
Gene Expression of MOSE cells at different transformation stages
|
| Sample_geo_accession | GSM610475
| | Sample_status | Public on Mar 03 2011
| | Sample_submission_date | Oct 19 2010
| | Sample_last_update_date | Mar 03 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_growth_protocol_ch1 | MOSE cell lines were routinely maintained in DMEM high glucose medium supplemented with 4% fetal bovine serum, 100 mg/ml each of penicillin and streptomycin, 5 mg/ml insulin, 5 mg/ml transferrin, and 5 ng/ml sodium selenite.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA samples for early (passage 13, 14, and 15), intermediate (63, 71, and 73), and late (136, 142, and 143) passages were isolated using the RNeasy Kit according to the manufacturers instructions and treated with ribonuclease-free deoxyribonuclease I. RNA samples were assayed on the Agilent 2100 BioAnalyzer for qualitative assessment and quantification.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA.
| | Sample_hyb_protocol | The RNA samples were processed using Affymetrix's GeneChip WT cDNA Synthesis and Amplification Kit (900672). Hybridization was done using Affymetrix Hybridization, Wash, and Stain Kit (part # 900720). Arrays were hybed for 16 hr at 45C. Samples were stained and washed using the Genechip Fluidics 450 station
| | Sample_scan_protocol | GeneChips were scanned using the GCS 3000 HR scanner.
| | Sample_data_processing | We utilized MicroArray Suite 5.0 (Affymetrix, Santa Clara, CA) to process raw microarray data. Data values were globally scaled to an average target intensity to allow inter-GeneChip comparisons.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Eva,M,Schmelz
| | Sample_contact_email | eschmelz@vt.edu
| | Sample_contact_laboratory | Schmelz
| | Sample_contact_department | HNFE
| | Sample_contact_institute | Virginia Tech
| | Sample_contact_address | Virginia Tech
| | Sample_contact_city | Blacksburg
| | Sample_contact_state | VA
| | Sample_contact_zip/postal_code | 24060
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM610nnn/GSM610475/suppl/GSM610475.CEL.gz
| | Sample_series_id | GSE24789
| | Sample_data_row_count | 45101
| | |
|
GSM610476 | GPL1261 |
|
MOSE Late Cells, biological replicate 1
|
mouse ovarian surface epithelial cells in culture
|
cell type: mouse ovarian surface epithelial (MOSE) cell
malignant potential: malignant
|
13_p2.136.CEL
Gene Expression of MOSE cells at different transformation stages
|
| Sample_geo_accession | GSM610476
| | Sample_status | Public on Mar 03 2011
| | Sample_submission_date | Oct 19 2010
| | Sample_last_update_date | Mar 03 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_growth_protocol_ch1 | MOSE cell lines were routinely maintained in DMEM high glucose medium supplemented with 4% fetal bovine serum, 100 mg/ml each of penicillin and streptomycin, 5 mg/ml insulin, 5 mg/ml transferrin, and 5 ng/ml sodium selenite.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA samples for early (passage 13, 14, and 15), intermediate (63, 71, and 73), and late (136, 142, and 143) passages were isolated using the RNeasy Kit according to the manufacturers instructions and treated with ribonuclease-free deoxyribonuclease I. RNA samples were assayed on the Agilent 2100 BioAnalyzer for qualitative assessment and quantification.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA.
| | Sample_hyb_protocol | The RNA samples were processed using Affymetrix's GeneChip WT cDNA Synthesis and Amplification Kit (900672). Hybridization was done using Affymetrix Hybridization, Wash, and Stain Kit (part # 900720). Arrays were hybed for 16 hr at 45C. Samples were stained and washed using the Genechip Fluidics 450 station
| | Sample_scan_protocol | GeneChips were scanned using the GCS 3000 HR scanner.
| | Sample_data_processing | We utilized MicroArray Suite 5.0 (Affymetrix, Santa Clara, CA) to process raw microarray data. Data values were globally scaled to an average target intensity to allow inter-GeneChip comparisons.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Eva,M,Schmelz
| | Sample_contact_email | eschmelz@vt.edu
| | Sample_contact_laboratory | Schmelz
| | Sample_contact_department | HNFE
| | Sample_contact_institute | Virginia Tech
| | Sample_contact_address | Virginia Tech
| | Sample_contact_city | Blacksburg
| | Sample_contact_state | VA
| | Sample_contact_zip/postal_code | 24060
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM610nnn/GSM610476/suppl/GSM610476.CEL.gz
| | Sample_series_id | GSE24789
| | Sample_data_row_count | 45101
| | |
|
GSM610477 | GPL1261 |
|
MOSE Late Cells, biological replicate 2
|
mouse ovarian surface epithelial cells in culture
|
cell type: mouse ovarian surface epithelial (MOSE) cell
malignant potential: malignant
|
14_p2.142.CEL
Gene Expression of MOSE cells at different transformation stages
|
| Sample_geo_accession | GSM610477
| | Sample_status | Public on Mar 03 2011
| | Sample_submission_date | Oct 19 2010
| | Sample_last_update_date | Mar 03 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_growth_protocol_ch1 | MOSE cell lines were routinely maintained in DMEM high glucose medium supplemented with 4% fetal bovine serum, 100 mg/ml each of penicillin and streptomycin, 5 mg/ml insulin, 5 mg/ml transferrin, and 5 ng/ml sodium selenite.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA samples for early (passage 13, 14, and 15), intermediate (63, 71, and 73), and late (136, 142, and 143) passages were isolated using the RNeasy Kit according to the manufacturers instructions and treated with ribonuclease-free deoxyribonuclease I. RNA samples were assayed on the Agilent 2100 BioAnalyzer for qualitative assessment and quantification.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA.
| | Sample_hyb_protocol | The RNA samples were processed using Affymetrix's GeneChip WT cDNA Synthesis and Amplification Kit (900672). Hybridization was done using Affymetrix Hybridization, Wash, and Stain Kit (part # 900720). Arrays were hybed for 16 hr at 45C. Samples were stained and washed using the Genechip Fluidics 450 station
| | Sample_scan_protocol | GeneChips were scanned using the GCS 3000 HR scanner.
| | Sample_data_processing | We utilized MicroArray Suite 5.0 (Affymetrix, Santa Clara, CA) to process raw microarray data. Data values were globally scaled to an average target intensity to allow inter-GeneChip comparisons.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Eva,M,Schmelz
| | Sample_contact_email | eschmelz@vt.edu
| | Sample_contact_laboratory | Schmelz
| | Sample_contact_department | HNFE
| | Sample_contact_institute | Virginia Tech
| | Sample_contact_address | Virginia Tech
| | Sample_contact_city | Blacksburg
| | Sample_contact_state | VA
| | Sample_contact_zip/postal_code | 24060
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM610nnn/GSM610477/suppl/GSM610477.CEL.gz
| | Sample_series_id | GSE24789
| | Sample_data_row_count | 45101
| | |
|
GSM610478 | GPL1261 |
|
MOSE Late Cells, biological replicate 3
|
mouse ovarian surface epithelial cells in culture
|
cell type: mouse ovarian surface epithelial (MOSE) cell
malignant potential: malignant
|
15_p2.143.CEL
Gene Expression of MOSE cells at different transformation stages
|
| Sample_geo_accession | GSM610478
| | Sample_status | Public on Mar 03 2011
| | Sample_submission_date | Oct 19 2010
| | Sample_last_update_date | Mar 03 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_growth_protocol_ch1 | MOSE cell lines were routinely maintained in DMEM high glucose medium supplemented with 4% fetal bovine serum, 100 mg/ml each of penicillin and streptomycin, 5 mg/ml insulin, 5 mg/ml transferrin, and 5 ng/ml sodium selenite.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA samples for early (passage 13, 14, and 15), intermediate (63, 71, and 73), and late (136, 142, and 143) passages were isolated using the RNeasy Kit according to the manufacturers instructions and treated with ribonuclease-free deoxyribonuclease I. RNA samples were assayed on the Agilent 2100 BioAnalyzer for qualitative assessment and quantification.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA.
| | Sample_hyb_protocol | The RNA samples were processed using Affymetrix's GeneChip WT cDNA Synthesis and Amplification Kit (900672). Hybridization was done using Affymetrix Hybridization, Wash, and Stain Kit (part # 900720). Arrays were hybed for 16 hr at 45C. Samples were stained and washed using the Genechip Fluidics 450 station
| | Sample_scan_protocol | GeneChips were scanned using the GCS 3000 HR scanner.
| | Sample_data_processing | We utilized MicroArray Suite 5.0 (Affymetrix, Santa Clara, CA) to process raw microarray data. Data values were globally scaled to an average target intensity to allow inter-GeneChip comparisons.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Eva,M,Schmelz
| | Sample_contact_email | eschmelz@vt.edu
| | Sample_contact_laboratory | Schmelz
| | Sample_contact_department | HNFE
| | Sample_contact_institute | Virginia Tech
| | Sample_contact_address | Virginia Tech
| | Sample_contact_city | Blacksburg
| | Sample_contact_state | VA
| | Sample_contact_zip/postal_code | 24060
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM610nnn/GSM610478/suppl/GSM610478.CEL.gz
| | Sample_series_id | GSE24789
| | Sample_data_row_count | 45101
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