Search results for the GEO ID: GSE24928  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM612931 | GPL1261 |
|
UGS at E17 from vehicle exposed fetus
|
Control, Mouse fetus UGS region at E17
|
strain: C57BL/6
agent: Control
age: E17
tissue: urogenital sinus
|
Gene expression data from vehicle exposed fetus UGS at E17
|
| Sample_geo_accession | GSM612931
| | Sample_status | Public on Dec 06 2010
| | Sample_submission_date | Oct 26 2010
| | Sample_last_update_date | Dec 06 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | The bladder and urethra were removed and dissected to isolate UGS. To purify UGS, other tissues such as the bladder, urethra, Wolffian duct (WD), seminal vesicle (SV), and Mullerian duct (MD) were removed from both the male and female urogenital tracts.
| | Sample_growth_protocol_ch1 | Pregnant female C57BL/6 mice were exposed with BPA (20 μg/kg/day) or synthetic estrogen Diethylstilbestrol (DES: 0.2 μg/kg/day), which were dissolved in tocopherol-stripped corn oil on embryonic day 13 (E13) to E16. Fetuses were collected at E17, E18, P0, and P1 to sample UGS region.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using RNeasy Mini Kit (Qiagen Inc., Valencia, CA, USA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | During the in vitro transcription using Bioarray kit (Enzo Biochem., Farmingdale, NY, USA), generated cRNAs were labeled with biotin-16-UTP and biotin-11-CTP.
| | Sample_hyb_protocol | Fragmented cRNAs were hybridized with the GeneChip Mouse Genome 430 ver. 2.0 (Affymetrix Inc., Santa Clara, CA, USA) at 45C for 18 h. GeneChips were stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | Stained GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G .
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | KATSUHIDE,,IGARASHI
| | Sample_contact_email | igarashi@nihs.go.jp
| | Sample_contact_phone | +81-3-3700-9672
| | Sample_contact_laboratory | Division of Cellular and Molecular Toxicology
| | Sample_contact_department | Biological Safety Research Center
| | Sample_contact_institute | National Institute of Health Sciences
| | Sample_contact_address | 1-18-1 Kamiyoga
| | Sample_contact_city | Setagaya-ku
| | Sample_contact_state | Tokyo
| | Sample_contact_zip/postal_code | 158-8501
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM612nnn/GSM612931/suppl/GSM612931.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM612nnn/GSM612931/suppl/GSM612931.CHP.gz
| | Sample_series_id | GSE24928
| | Sample_data_row_count | 45101
| | |
|
GSM612932 | GPL1261 |
|
UGS at E18 from vehicle exposed fetus
|
Control, Mouse fetus UGS region at E18
|
strain: C57BL/6
agent: Control
age: E18
tissue: urogenital sinus
|
Gene expression data from vehicle exposed fetus UGS at E18
|
| Sample_geo_accession | GSM612932
| | Sample_status | Public on Dec 06 2010
| | Sample_submission_date | Oct 26 2010
| | Sample_last_update_date | Dec 06 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | The bladder and urethra were removed and dissected to isolate UGS. To purify UGS, other tissues such as the bladder, urethra, Wolffian duct (WD), seminal vesicle (SV), and Mullerian duct (MD) were removed from both the male and female urogenital tracts.
| | Sample_growth_protocol_ch1 | Pregnant female C57BL/6 mice were exposed with BPA (20 μg/kg/day) or synthetic estrogen Diethylstilbestrol (DES: 0.2 μg/kg/day), which were dissolved in tocopherol-stripped corn oil on embryonic day 13 (E13) to E16. Fetuses were collected at E17, E18, P0, and P1 to sample UGS region.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using RNeasy Mini Kit (Qiagen Inc., Valencia, CA, USA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | During the in vitro transcription using Bioarray kit (Enzo Biochem., Farmingdale, NY, USA), generated cRNAs were labeled with biotin-16-UTP and biotin-11-CTP.
| | Sample_hyb_protocol | Fragmented cRNAs were hybridized with the GeneChip Mouse Genome 430 ver. 2.0 (Affymetrix Inc., Santa Clara, CA, USA) at 45C for 18 h. GeneChips were stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | Stained GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G .
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | KATSUHIDE,,IGARASHI
| | Sample_contact_email | igarashi@nihs.go.jp
| | Sample_contact_phone | +81-3-3700-9672
| | Sample_contact_laboratory | Division of Cellular and Molecular Toxicology
| | Sample_contact_department | Biological Safety Research Center
| | Sample_contact_institute | National Institute of Health Sciences
| | Sample_contact_address | 1-18-1 Kamiyoga
| | Sample_contact_city | Setagaya-ku
| | Sample_contact_state | Tokyo
| | Sample_contact_zip/postal_code | 158-8501
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM612nnn/GSM612932/suppl/GSM612932.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM612nnn/GSM612932/suppl/GSM612932.CHP.gz
| | Sample_series_id | GSE24928
| | Sample_data_row_count | 45101
| | |
|
GSM612933 | GPL1261 |
|
UGS at P0 from vehicle exposed fetus
|
Control, Mouse fetus UGS region at P0
|
strain: C57BL/6
agent: Control
age: P0
tissue: urogenital sinus
|
Gene expression data from vehicle exposed fetus UGS at P0
|
| Sample_geo_accession | GSM612933
| | Sample_status | Public on Dec 06 2010
| | Sample_submission_date | Oct 26 2010
| | Sample_last_update_date | Dec 06 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | The bladder and urethra were removed and dissected to isolate UGS. To purify UGS, other tissues such as the bladder, urethra, Wolffian duct (WD), seminal vesicle (SV), and Mullerian duct (MD) were removed from both the male and female urogenital tracts.
| | Sample_growth_protocol_ch1 | Pregnant female C57BL/6 mice were exposed with BPA (20 μg/kg/day) or synthetic estrogen Diethylstilbestrol (DES: 0.2 μg/kg/day), which were dissolved in tocopherol-stripped corn oil on embryonic day 13 (E13) to E16. Fetuses were collected at E17, E18, P0, and P1 to sample UGS region.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using RNeasy Mini Kit (Qiagen Inc., Valencia, CA, USA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | During the in vitro transcription using Bioarray kit (Enzo Biochem., Farmingdale, NY, USA), generated cRNAs were labeled with biotin-16-UTP and biotin-11-CTP.
| | Sample_hyb_protocol | Fragmented cRNAs were hybridized with the GeneChip Mouse Genome 430 ver. 2.0 (Affymetrix Inc., Santa Clara, CA, USA) at 45C for 18 h. GeneChips were stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | Stained GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G .
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | KATSUHIDE,,IGARASHI
| | Sample_contact_email | igarashi@nihs.go.jp
| | Sample_contact_phone | +81-3-3700-9672
| | Sample_contact_laboratory | Division of Cellular and Molecular Toxicology
| | Sample_contact_department | Biological Safety Research Center
| | Sample_contact_institute | National Institute of Health Sciences
| | Sample_contact_address | 1-18-1 Kamiyoga
| | Sample_contact_city | Setagaya-ku
| | Sample_contact_state | Tokyo
| | Sample_contact_zip/postal_code | 158-8501
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM612nnn/GSM612933/suppl/GSM612933.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM612nnn/GSM612933/suppl/GSM612933.CHP.gz
| | Sample_series_id | GSE24928
| | Sample_data_row_count | 45101
| | |
|
GSM612934 | GPL1261 |
|
UGS at E17 from DES exposed fetus
|
DES-exposed, Mouse fetus UGS region at E17
|
strain: C57BL/6
agent: DES
age: E17
tissue: urogenital sinus
|
Gene expression data from DES exposed fetus UGS at E17
|
| Sample_geo_accession | GSM612934
| | Sample_status | Public on Dec 06 2010
| | Sample_submission_date | Oct 26 2010
| | Sample_last_update_date | Dec 06 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | The bladder and urethra were removed and dissected to isolate UGS. To purify UGS, other tissues such as the bladder, urethra, Wolffian duct (WD), seminal vesicle (SV), and Mullerian duct (MD) were removed from both the male and female urogenital tracts.
| | Sample_growth_protocol_ch1 | Pregnant female C57BL/6 mice were exposed with BPA (20 μg/kg/day) or synthetic estrogen Diethylstilbestrol (DES: 0.2 μg/kg/day), which were dissolved in tocopherol-stripped corn oil on embryonic day 13 (E13) to E16. Fetuses were collected at E17, E18, P0, and P1 to sample UGS region.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using RNeasy Mini Kit (Qiagen Inc., Valencia, CA, USA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | During the in vitro transcription using Bioarray kit (Enzo Biochem., Farmingdale, NY, USA), generated cRNAs were labeled with biotin-16-UTP and biotin-11-CTP.
| | Sample_hyb_protocol | Fragmented cRNAs were hybridized with the GeneChip Mouse Genome 430 ver. 2.0 (Affymetrix Inc., Santa Clara, CA, USA) at 45C for 18 h. GeneChips were stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | Stained GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G .
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | KATSUHIDE,,IGARASHI
| | Sample_contact_email | igarashi@nihs.go.jp
| | Sample_contact_phone | +81-3-3700-9672
| | Sample_contact_laboratory | Division of Cellular and Molecular Toxicology
| | Sample_contact_department | Biological Safety Research Center
| | Sample_contact_institute | National Institute of Health Sciences
| | Sample_contact_address | 1-18-1 Kamiyoga
| | Sample_contact_city | Setagaya-ku
| | Sample_contact_state | Tokyo
| | Sample_contact_zip/postal_code | 158-8501
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM612nnn/GSM612934/suppl/GSM612934.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM612nnn/GSM612934/suppl/GSM612934.CHP.gz
| | Sample_series_id | GSE24928
| | Sample_data_row_count | 45101
| | |
|
GSM612935 | GPL1261 |
|
UGS at E18 from DES exposed fetus
|
DES-exposed, Mouse fetus UGS region at E18
|
strain: C57BL/6
agent: DES
age: E18
tissue: urogenital sinus
|
Gene expression data from DES exposed fetus UGS at E18
|
| Sample_geo_accession | GSM612935
| | Sample_status | Public on Dec 06 2010
| | Sample_submission_date | Oct 26 2010
| | Sample_last_update_date | Dec 06 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | The bladder and urethra were removed and dissected to isolate UGS. To purify UGS, other tissues such as the bladder, urethra, Wolffian duct (WD), seminal vesicle (SV), and Mullerian duct (MD) were removed from both the male and female urogenital tracts.
| | Sample_growth_protocol_ch1 | Pregnant female C57BL/6 mice were exposed with BPA (20 μg/kg/day) or synthetic estrogen Diethylstilbestrol (DES: 0.2 μg/kg/day), which were dissolved in tocopherol-stripped corn oil on embryonic day 13 (E13) to E16. Fetuses were collected at E17, E18, P0, and P1 to sample UGS region.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using RNeasy Mini Kit (Qiagen Inc., Valencia, CA, USA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | During the in vitro transcription using Bioarray kit (Enzo Biochem., Farmingdale, NY, USA), generated cRNAs were labeled with biotin-16-UTP and biotin-11-CTP.
| | Sample_hyb_protocol | Fragmented cRNAs were hybridized with the GeneChip Mouse Genome 430 ver. 2.0 (Affymetrix Inc., Santa Clara, CA, USA) at 45C for 18 h. GeneChips were stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | Stained GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G .
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | KATSUHIDE,,IGARASHI
| | Sample_contact_email | igarashi@nihs.go.jp
| | Sample_contact_phone | +81-3-3700-9672
| | Sample_contact_laboratory | Division of Cellular and Molecular Toxicology
| | Sample_contact_department | Biological Safety Research Center
| | Sample_contact_institute | National Institute of Health Sciences
| | Sample_contact_address | 1-18-1 Kamiyoga
| | Sample_contact_city | Setagaya-ku
| | Sample_contact_state | Tokyo
| | Sample_contact_zip/postal_code | 158-8501
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM612nnn/GSM612935/suppl/GSM612935.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM612nnn/GSM612935/suppl/GSM612935.CHP.gz
| | Sample_series_id | GSE24928
| | Sample_data_row_count | 45101
| | |
|
GSM612936 | GPL1261 |
|
UGS at P0 from DES exposed fetus
|
DES-exposed, Mouse fetus UGS region at P0
|
strain: C57BL/6
agent: DES
age: P0
tissue: urogenital sinus
|
Gene expression data from DES exposed fetus UGS at P0
|
| Sample_geo_accession | GSM612936
| | Sample_status | Public on Dec 06 2010
| | Sample_submission_date | Oct 26 2010
| | Sample_last_update_date | Dec 06 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | The bladder and urethra were removed and dissected to isolate UGS. To purify UGS, other tissues such as the bladder, urethra, Wolffian duct (WD), seminal vesicle (SV), and Mullerian duct (MD) were removed from both the male and female urogenital tracts.
| | Sample_growth_protocol_ch1 | Pregnant female C57BL/6 mice were exposed with BPA (20 μg/kg/day) or synthetic estrogen Diethylstilbestrol (DES: 0.2 μg/kg/day), which were dissolved in tocopherol-stripped corn oil on embryonic day 13 (E13) to E16. Fetuses were collected at E17, E18, P0, and P1 to sample UGS region.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using RNeasy Mini Kit (Qiagen Inc., Valencia, CA, USA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | During the in vitro transcription using Bioarray kit (Enzo Biochem., Farmingdale, NY, USA), generated cRNAs were labeled with biotin-16-UTP and biotin-11-CTP.
| | Sample_hyb_protocol | Fragmented cRNAs were hybridized with the GeneChip Mouse Genome 430 ver. 2.0 (Affymetrix Inc., Santa Clara, CA, USA) at 45C for 18 h. GeneChips were stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | Stained GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G .
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | KATSUHIDE,,IGARASHI
| | Sample_contact_email | igarashi@nihs.go.jp
| | Sample_contact_phone | +81-3-3700-9672
| | Sample_contact_laboratory | Division of Cellular and Molecular Toxicology
| | Sample_contact_department | Biological Safety Research Center
| | Sample_contact_institute | National Institute of Health Sciences
| | Sample_contact_address | 1-18-1 Kamiyoga
| | Sample_contact_city | Setagaya-ku
| | Sample_contact_state | Tokyo
| | Sample_contact_zip/postal_code | 158-8501
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM612nnn/GSM612936/suppl/GSM612936.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM612nnn/GSM612936/suppl/GSM612936.CHP.gz
| | Sample_series_id | GSE24928
| | Sample_data_row_count | 45101
| | |
|
GSM612937 | GPL1261 |
|
UGS at E17 from BPA exposed fetus
|
BPA-exposed, Mouse fetus UGS region at E17
|
strain: C57BL/6
agent: BPA
age: E17
tissue: urogenital sinus
|
Gene expression data from BPA exposed fetus UGS at E17
|
| Sample_geo_accession | GSM612937
| | Sample_status | Public on Dec 06 2010
| | Sample_submission_date | Oct 26 2010
| | Sample_last_update_date | Dec 06 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | The bladder and urethra were removed and dissected to isolate UGS. To purify UGS, other tissues such as the bladder, urethra, Wolffian duct (WD), seminal vesicle (SV), and Mullerian duct (MD) were removed from both the male and female urogenital tracts.
| | Sample_growth_protocol_ch1 | Pregnant female C57BL/6 mice were exposed with BPA (20 μg/kg/day) or synthetic estrogen Diethylstilbestrol (DES: 0.2 μg/kg/day), which were dissolved in tocopherol-stripped corn oil on embryonic day 13 (E13) to E16. Fetuses were collected at E17, E18, P0, and P1 to sample UGS region.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using RNeasy Mini Kit (Qiagen Inc., Valencia, CA, USA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | During the in vitro transcription using Bioarray kit (Enzo Biochem., Farmingdale, NY, USA), generated cRNAs were labeled with biotin-16-UTP and biotin-11-CTP.
| | Sample_hyb_protocol | Fragmented cRNAs were hybridized with the GeneChip Mouse Genome 430 ver. 2.0 (Affymetrix Inc., Santa Clara, CA, USA) at 45C for 18 h. GeneChips were stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | Stained GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G .
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | KATSUHIDE,,IGARASHI
| | Sample_contact_email | igarashi@nihs.go.jp
| | Sample_contact_phone | +81-3-3700-9672
| | Sample_contact_laboratory | Division of Cellular and Molecular Toxicology
| | Sample_contact_department | Biological Safety Research Center
| | Sample_contact_institute | National Institute of Health Sciences
| | Sample_contact_address | 1-18-1 Kamiyoga
| | Sample_contact_city | Setagaya-ku
| | Sample_contact_state | Tokyo
| | Sample_contact_zip/postal_code | 158-8501
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM612nnn/GSM612937/suppl/GSM612937.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM612nnn/GSM612937/suppl/GSM612937.CHP.gz
| | Sample_series_id | GSE24928
| | Sample_data_row_count | 45101
| | |
|
GSM612938 | GPL1261 |
|
UGS at E18 from BPA exposed fetus
|
BPA-exposed, Mouse fetus UGS region at E18
|
strain: C57BL/6
agent: BPA
age: E18
tissue: urogenital sinus
|
Gene expression data from BPA exposed fetus UGS at E18
|
| Sample_geo_accession | GSM612938
| | Sample_status | Public on Dec 06 2010
| | Sample_submission_date | Oct 26 2010
| | Sample_last_update_date | Dec 06 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | The bladder and urethra were removed and dissected to isolate UGS. To purify UGS, other tissues such as the bladder, urethra, Wolffian duct (WD), seminal vesicle (SV), and Mullerian duct (MD) were removed from both the male and female urogenital tracts.
| | Sample_growth_protocol_ch1 | Pregnant female C57BL/6 mice were exposed with BPA (20 μg/kg/day) or synthetic estrogen Diethylstilbestrol (DES: 0.2 μg/kg/day), which were dissolved in tocopherol-stripped corn oil on embryonic day 13 (E13) to E16. Fetuses were collected at E17, E18, P0, and P1 to sample UGS region.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using RNeasy Mini Kit (Qiagen Inc., Valencia, CA, USA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | During the in vitro transcription using Bioarray kit (Enzo Biochem., Farmingdale, NY, USA), generated cRNAs were labeled with biotin-16-UTP and biotin-11-CTP.
| | Sample_hyb_protocol | Fragmented cRNAs were hybridized with the GeneChip Mouse Genome 430 ver. 2.0 (Affymetrix Inc., Santa Clara, CA, USA) at 45C for 18 h. GeneChips were stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | Stained GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G .
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | KATSUHIDE,,IGARASHI
| | Sample_contact_email | igarashi@nihs.go.jp
| | Sample_contact_phone | +81-3-3700-9672
| | Sample_contact_laboratory | Division of Cellular and Molecular Toxicology
| | Sample_contact_department | Biological Safety Research Center
| | Sample_contact_institute | National Institute of Health Sciences
| | Sample_contact_address | 1-18-1 Kamiyoga
| | Sample_contact_city | Setagaya-ku
| | Sample_contact_state | Tokyo
| | Sample_contact_zip/postal_code | 158-8501
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM612nnn/GSM612938/suppl/GSM612938.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM612nnn/GSM612938/suppl/GSM612938.CHP.gz
| | Sample_series_id | GSE24928
| | Sample_data_row_count | 45101
| | |
|
GSM612939 | GPL1261 |
|
UGS at P0 from BPA exposed fetus
|
BPA-exposed, Mouse fetus UGS region at P0
|
strain: C57BL/6
agent: BPA
age: P0
tissue: urogenital sinus
|
Gene expression data from BPA exposed fetus UGS at P0
|
| Sample_geo_accession | GSM612939
| | Sample_status | Public on Dec 06 2010
| | Sample_submission_date | Oct 26 2010
| | Sample_last_update_date | Dec 06 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | The bladder and urethra were removed and dissected to isolate UGS. To purify UGS, other tissues such as the bladder, urethra, Wolffian duct (WD), seminal vesicle (SV), and Mullerian duct (MD) were removed from both the male and female urogenital tracts.
| | Sample_growth_protocol_ch1 | Pregnant female C57BL/6 mice were exposed with BPA (20 μg/kg/day) or synthetic estrogen Diethylstilbestrol (DES: 0.2 μg/kg/day), which were dissolved in tocopherol-stripped corn oil on embryonic day 13 (E13) to E16. Fetuses were collected at E17, E18, P0, and P1 to sample UGS region.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted using RNeasy Mini Kit (Qiagen Inc., Valencia, CA, USA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | During the in vitro transcription using Bioarray kit (Enzo Biochem., Farmingdale, NY, USA), generated cRNAs were labeled with biotin-16-UTP and biotin-11-CTP.
| | Sample_hyb_protocol | Fragmented cRNAs were hybridized with the GeneChip Mouse Genome 430 ver. 2.0 (Affymetrix Inc., Santa Clara, CA, USA) at 45C for 18 h. GeneChips were stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | Stained GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G .
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | KATSUHIDE,,IGARASHI
| | Sample_contact_email | igarashi@nihs.go.jp
| | Sample_contact_phone | +81-3-3700-9672
| | Sample_contact_laboratory | Division of Cellular and Molecular Toxicology
| | Sample_contact_department | Biological Safety Research Center
| | Sample_contact_institute | National Institute of Health Sciences
| | Sample_contact_address | 1-18-1 Kamiyoga
| | Sample_contact_city | Setagaya-ku
| | Sample_contact_state | Tokyo
| | Sample_contact_zip/postal_code | 158-8501
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM612nnn/GSM612939/suppl/GSM612939.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM612nnn/GSM612939/suppl/GSM612939.CHP.gz
| | Sample_series_id | GSE24928
| | Sample_data_row_count | 45101
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