Search results for the GEO ID: GSE25014  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM614501 | GPL570 |
|
PMVECs treated with vehicle rep 1
|
cultured lung endothelial cells
|
cell type: pulmonary microvascular endothelial cells
treatment: control
|
Gene expression data of PMVECs cultured for seven days in normal medium
|
| Sample_geo_accession | GSM614501
| | Sample_status | Public on Oct 13 2011
| | Sample_submission_date | Oct 29 2010
| | Sample_last_update_date | Oct 13 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were treated with freshly prepared heme (5 micromolar), every 48 hours for seven days
| | Sample_growth_protocol_ch1 | Endothelial cells were grown in complete medium containing fetal calf serum at 37 degrees celcius
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on a Human U 133 plus 2.0 GeneChip Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | Gene level expression index was generated using the Affymetrix MAS 5.0 software.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Tianwei,,Yu
| | Sample_contact_department | Biostatistics
| | Sample_contact_institute | Emory University
| | Sample_contact_address | 1518 Clifton Rd NE
| | Sample_contact_city | Atlanta
| | Sample_contact_state | GA
| | Sample_contact_zip/postal_code | 30322
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM614nnn/GSM614501/suppl/GSM614501_Sample_1.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM614nnn/GSM614501/suppl/GSM614501_Sample_1.CHP.gz
| | Sample_series_id | GSE25014
| | Sample_data_row_count | 54675
| | |
|
GSM614502 | GPL570 |
|
PMVECs treated with vehicle rep2
|
cultured lung endothelial cells
|
cell type: pulmonary microvascular endothelial cells
treatment: control
|
Gene expression data of PMVECs cultured for seven days in normal medium
|
| Sample_geo_accession | GSM614502
| | Sample_status | Public on Oct 13 2011
| | Sample_submission_date | Oct 29 2010
| | Sample_last_update_date | Oct 13 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were treated with freshly prepared heme (5 micromolar), every 48 hours for seven days
| | Sample_growth_protocol_ch1 | Endothelial cells were grown in complete medium containing fetal calf serum at 37 degrees celcius
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on a Human U 133 plus 2.0 GeneChip Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | Gene level expression index was generated using the Affymetrix MAS 5.0 software.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Tianwei,,Yu
| | Sample_contact_department | Biostatistics
| | Sample_contact_institute | Emory University
| | Sample_contact_address | 1518 Clifton Rd NE
| | Sample_contact_city | Atlanta
| | Sample_contact_state | GA
| | Sample_contact_zip/postal_code | 30322
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM614nnn/GSM614502/suppl/GSM614502_Sample_2.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM614nnn/GSM614502/suppl/GSM614502_Sample_2.CHP.gz
| | Sample_series_id | GSE25014
| | Sample_data_row_count | 54675
| | |
|
GSM614503 | GPL570 |
|
PMVECs treated with vehicle rep 3
|
cultured lung endothelial cells
|
cell type: pulmonary microvascular endothelial cells
treatment: control
|
Gene expression data of PMVECs cultured for seven days in normal medium
|
| Sample_geo_accession | GSM614503
| | Sample_status | Public on Oct 13 2011
| | Sample_submission_date | Oct 29 2010
| | Sample_last_update_date | Oct 13 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were treated with freshly prepared heme (5 micromolar), every 48 hours for seven days
| | Sample_growth_protocol_ch1 | Endothelial cells were grown in complete medium containing fetal calf serum at 37 degrees celcius
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on a Human U 133 plus 2.0 GeneChip Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | Gene level expression index was generated using the Affymetrix MAS 5.0 software.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Tianwei,,Yu
| | Sample_contact_department | Biostatistics
| | Sample_contact_institute | Emory University
| | Sample_contact_address | 1518 Clifton Rd NE
| | Sample_contact_city | Atlanta
| | Sample_contact_state | GA
| | Sample_contact_zip/postal_code | 30322
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM614nnn/GSM614503/suppl/GSM614503_Sample_3.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM614nnn/GSM614503/suppl/GSM614503_Sample_3.CHP.gz
| | Sample_series_id | GSE25014
| | Sample_data_row_count | 54675
| | |
|
GSM614504 | GPL570 |
|
PMVECs treated with vehicle rep 4
|
cultured lung endothelial cells
|
cell type: pulmonary microvascular endothelial cells
treatment: control
|
Gene expression data of PMVECs cultured for seven days in normal medium
|
| Sample_geo_accession | GSM614504
| | Sample_status | Public on Oct 13 2011
| | Sample_submission_date | Oct 29 2010
| | Sample_last_update_date | Oct 13 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were treated with freshly prepared heme (5 micromolar), every 48 hours for seven days
| | Sample_growth_protocol_ch1 | Endothelial cells were grown in complete medium containing fetal calf serum at 37 degrees celcius
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on a Human U 133 plus 2.0 GeneChip Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | Gene level expression index was generated using the Affymetrix MAS 5.0 software.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Tianwei,,Yu
| | Sample_contact_department | Biostatistics
| | Sample_contact_institute | Emory University
| | Sample_contact_address | 1518 Clifton Rd NE
| | Sample_contact_city | Atlanta
| | Sample_contact_state | GA
| | Sample_contact_zip/postal_code | 30322
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM614nnn/GSM614504/suppl/GSM614504_Sample_4.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM614nnn/GSM614504/suppl/GSM614504_Sample_4.CHP.gz
| | Sample_series_id | GSE25014
| | Sample_data_row_count | 54675
| | |
|
GSM614505 | GPL570 |
|
PMVECs treated with vehicle rep 5
|
cultured lung endothelial cells
|
cell type: pulmonary microvascular endothelial cells
treatment: control
|
Gene expression data of PMVECs cultured for seven days in normal medium
|
| Sample_geo_accession | GSM614505
| | Sample_status | Public on Oct 13 2011
| | Sample_submission_date | Oct 29 2010
| | Sample_last_update_date | Oct 13 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were treated with freshly prepared heme (5 micromolar), every 48 hours for seven days
| | Sample_growth_protocol_ch1 | Endothelial cells were grown in complete medium containing fetal calf serum at 37 degrees celcius
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on a Human U 133 plus 2.0 GeneChip Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | Gene level expression index was generated using the Affymetrix MAS 5.0 software.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Tianwei,,Yu
| | Sample_contact_department | Biostatistics
| | Sample_contact_institute | Emory University
| | Sample_contact_address | 1518 Clifton Rd NE
| | Sample_contact_city | Atlanta
| | Sample_contact_state | GA
| | Sample_contact_zip/postal_code | 30322
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM614nnn/GSM614505/suppl/GSM614505_Sample_5.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM614nnn/GSM614505/suppl/GSM614505_Sample_5.CHP.gz
| | Sample_series_id | GSE25014
| | Sample_data_row_count | 54675
| | |
|
GSM614506 | GPL570 |
|
PMVECs treated with vehicle rep 6
|
cultured lung endothelial cells
|
cell type: pulmonary microvascular endothelial cells
treatment: control
|
Gene expression data of PMVECs cultured for seven days in normal medium
|
| Sample_geo_accession | GSM614506
| | Sample_status | Public on Oct 13 2011
| | Sample_submission_date | Oct 29 2010
| | Sample_last_update_date | Oct 13 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were treated with freshly prepared heme (5 micromolar), every 48 hours for seven days
| | Sample_growth_protocol_ch1 | Endothelial cells were grown in complete medium containing fetal calf serum at 37 degrees celcius
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on a Human U 133 plus 2.0 GeneChip Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | Gene level expression index was generated using the Affymetrix MAS 5.0 software.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Tianwei,,Yu
| | Sample_contact_department | Biostatistics
| | Sample_contact_institute | Emory University
| | Sample_contact_address | 1518 Clifton Rd NE
| | Sample_contact_city | Atlanta
| | Sample_contact_state | GA
| | Sample_contact_zip/postal_code | 30322
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM614nnn/GSM614506/suppl/GSM614506_Sample_6.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM614nnn/GSM614506/suppl/GSM614506_Sample_6.CHP.gz
| | Sample_series_id | GSE25014
| | Sample_data_row_count | 54675
| | |
|
GSM614507 | GPL570 |
|
PMVECs treated with 5 micromolar heme rep 1
|
cultured lung endothelial cells
|
cell type: pulmonary microvascular endothelial cells
treatment: heme
|
Gene expression data of PMVECs cultured for seven days in five micromolar heme
|
| Sample_geo_accession | GSM614507
| | Sample_status | Public on Oct 13 2011
| | Sample_submission_date | Oct 29 2010
| | Sample_last_update_date | Oct 13 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were treated with freshly prepared heme (5 micromolar), every 48 hours for seven days
| | Sample_growth_protocol_ch1 | Endothelial cells were grown in complete medium containing fetal calf serum at 37 degrees celcius
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on a Human U 133 plus 2.0 GeneChip Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | Gene level expression index was generated using the Affymetrix MAS 5.0 software.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Tianwei,,Yu
| | Sample_contact_department | Biostatistics
| | Sample_contact_institute | Emory University
| | Sample_contact_address | 1518 Clifton Rd NE
| | Sample_contact_city | Atlanta
| | Sample_contact_state | GA
| | Sample_contact_zip/postal_code | 30322
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM614nnn/GSM614507/suppl/GSM614507_Sample_7.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM614nnn/GSM614507/suppl/GSM614507_Sample_7.CHP.gz
| | Sample_series_id | GSE25014
| | Sample_data_row_count | 54675
| | |
|
GSM614508 | GPL570 |
|
PMVECs treated with 5 micromolar heme rep 2
|
cultured lung endothelial cells
|
cell type: pulmonary microvascular endothelial cells
treatment: heme
|
Gene expression data of PMVECs cultured for seven days in five micromolar heme
|
| Sample_geo_accession | GSM614508
| | Sample_status | Public on Oct 13 2011
| | Sample_submission_date | Oct 29 2010
| | Sample_last_update_date | Oct 13 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were treated with freshly prepared heme (5 micromolar), every 48 hours for seven days
| | Sample_growth_protocol_ch1 | Endothelial cells were grown in complete medium containing fetal calf serum at 37 degrees celcius
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on a Human U 133 plus 2.0 GeneChip Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | Gene level expression index was generated using the Affymetrix MAS 5.0 software.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Tianwei,,Yu
| | Sample_contact_department | Biostatistics
| | Sample_contact_institute | Emory University
| | Sample_contact_address | 1518 Clifton Rd NE
| | Sample_contact_city | Atlanta
| | Sample_contact_state | GA
| | Sample_contact_zip/postal_code | 30322
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM614nnn/GSM614508/suppl/GSM614508_Sample_8.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM614nnn/GSM614508/suppl/GSM614508_Sample_8.CHP.gz
| | Sample_series_id | GSE25014
| | Sample_data_row_count | 54675
| | |
|
GSM614509 | GPL570 |
|
PMVECs treated with 5 micromolar heme rep 3
|
cultured lung endothelial cells
|
cell type: pulmonary microvascular endothelial cells
treatment: heme
|
Gene expression data of PMVECs cultured for seven days in five micromolar heme
|
| Sample_geo_accession | GSM614509
| | Sample_status | Public on Oct 13 2011
| | Sample_submission_date | Oct 29 2010
| | Sample_last_update_date | Oct 13 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were treated with freshly prepared heme (5 micromolar), every 48 hours for seven days
| | Sample_growth_protocol_ch1 | Endothelial cells were grown in complete medium containing fetal calf serum at 37 degrees celcius
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on a Human U 133 plus 2.0 GeneChip Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | Gene level expression index was generated using the Affymetrix MAS 5.0 software.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Tianwei,,Yu
| | Sample_contact_department | Biostatistics
| | Sample_contact_institute | Emory University
| | Sample_contact_address | 1518 Clifton Rd NE
| | Sample_contact_city | Atlanta
| | Sample_contact_state | GA
| | Sample_contact_zip/postal_code | 30322
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM614nnn/GSM614509/suppl/GSM614509_Sample_9.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM614nnn/GSM614509/suppl/GSM614509_Sample_9.CHP.gz
| | Sample_series_id | GSE25014
| | Sample_data_row_count | 54675
| | |
|
GSM614510 | GPL570 |
|
PMVECs treated with 5 micromolar heme rep 4
|
cultured lung endothelial cells
|
cell type: pulmonary microvascular endothelial cells
treatment: heme
|
Gene expression data of PMVECs cultured for seven days in five micromolar heme
|
| Sample_geo_accession | GSM614510
| | Sample_status | Public on Oct 13 2011
| | Sample_submission_date | Oct 29 2010
| | Sample_last_update_date | Oct 13 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were treated with freshly prepared heme (5 micromolar), every 48 hours for seven days
| | Sample_growth_protocol_ch1 | Endothelial cells were grown in complete medium containing fetal calf serum at 37 degrees celcius
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on a Human U 133 plus 2.0 GeneChip Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | Gene level expression index was generated using the Affymetrix MAS 5.0 software.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Tianwei,,Yu
| | Sample_contact_department | Biostatistics
| | Sample_contact_institute | Emory University
| | Sample_contact_address | 1518 Clifton Rd NE
| | Sample_contact_city | Atlanta
| | Sample_contact_state | GA
| | Sample_contact_zip/postal_code | 30322
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM614nnn/GSM614510/suppl/GSM614510_Sample_10.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM614nnn/GSM614510/suppl/GSM614510_Sample_10.CHP.gz
| | Sample_series_id | GSE25014
| | Sample_data_row_count | 54675
| | |
|
GSM614511 | GPL570 |
|
PMVECs treated with 5 micromolar heme rep 5
|
cultured lung endothelial cells
|
cell type: pulmonary microvascular endothelial cells
treatment: heme
|
Gene expression data of PMVECs cultured for seven days in five micromolar heme
|
| Sample_geo_accession | GSM614511
| | Sample_status | Public on Oct 13 2011
| | Sample_submission_date | Oct 29 2010
| | Sample_last_update_date | Oct 13 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were treated with freshly prepared heme (5 micromolar), every 48 hours for seven days
| | Sample_growth_protocol_ch1 | Endothelial cells were grown in complete medium containing fetal calf serum at 37 degrees celcius
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on a Human U 133 plus 2.0 GeneChip Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | Gene level expression index was generated using the Affymetrix MAS 5.0 software.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Tianwei,,Yu
| | Sample_contact_department | Biostatistics
| | Sample_contact_institute | Emory University
| | Sample_contact_address | 1518 Clifton Rd NE
| | Sample_contact_city | Atlanta
| | Sample_contact_state | GA
| | Sample_contact_zip/postal_code | 30322
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM614nnn/GSM614511/suppl/GSM614511_Sample_11.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM614nnn/GSM614511/suppl/GSM614511_Sample_11.CHP.gz
| | Sample_series_id | GSE25014
| | Sample_data_row_count | 54675
| | |
|
GSM614512 | GPL570 |
|
PMVECs treated with 5 micromolar heme rep 6
|
cultured lung endothelial cells
|
cell type: pulmonary microvascular endothelial cells
treatment: heme
|
Gene expression data of PMVECs cultured for seven days in five micromolar heme
|
| Sample_geo_accession | GSM614512
| | Sample_status | Public on Oct 13 2011
| | Sample_submission_date | Oct 29 2010
| | Sample_last_update_date | Oct 13 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were treated with freshly prepared heme (5 micromolar), every 48 hours for seven days
| | Sample_growth_protocol_ch1 | Endothelial cells were grown in complete medium containing fetal calf serum at 37 degrees celcius
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on a Human U 133 plus 2.0 GeneChip Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | Gene level expression index was generated using the Affymetrix MAS 5.0 software.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Tianwei,,Yu
| | Sample_contact_department | Biostatistics
| | Sample_contact_institute | Emory University
| | Sample_contact_address | 1518 Clifton Rd NE
| | Sample_contact_city | Atlanta
| | Sample_contact_state | GA
| | Sample_contact_zip/postal_code | 30322
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM614nnn/GSM614512/suppl/GSM614512_Sample_12.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM614nnn/GSM614512/suppl/GSM614512_Sample_12.CHP.gz
| | Sample_series_id | GSE25014
| | Sample_data_row_count | 54675
| | |
|
GSM614513 | GPL570 |
|
PAECs treated with vehicle rep 1
|
cultured lung endothelial cells
|
cell type: pulmonary artery endothelial cells
treatment: control
|
Gene expression data of PAECs cultured for seven days in normal medium
|
| Sample_geo_accession | GSM614513
| | Sample_status | Public on Oct 13 2011
| | Sample_submission_date | Oct 29 2010
| | Sample_last_update_date | Oct 13 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were treated with freshly prepared heme (5 micromolar), every 48 hours for seven days
| | Sample_growth_protocol_ch1 | Endothelial cells were grown in complete medium containing fetal calf serum at 37 degrees celcius
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on a Human U 133 plus 2.0 GeneChip Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | Gene level expression index was generated using the Affymetrix MAS 5.0 software.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Tianwei,,Yu
| | Sample_contact_department | Biostatistics
| | Sample_contact_institute | Emory University
| | Sample_contact_address | 1518 Clifton Rd NE
| | Sample_contact_city | Atlanta
| | Sample_contact_state | GA
| | Sample_contact_zip/postal_code | 30322
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM614nnn/GSM614513/suppl/GSM614513_Sample_13.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM614nnn/GSM614513/suppl/GSM614513_Sample_13.CHP.gz
| | Sample_series_id | GSE25014
| | Sample_data_row_count | 54675
| | |
|
GSM614514 | GPL570 |
|
PAECs treated with vehicle rep 2
|
cultured lung endothelial cells
|
cell type: pulmonary artery endothelial cells
treatment: control
|
Gene expression data of PAECs cultured for seven days in normal medium
|
| Sample_geo_accession | GSM614514
| | Sample_status | Public on Oct 13 2011
| | Sample_submission_date | Oct 29 2010
| | Sample_last_update_date | Oct 13 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were treated with freshly prepared heme (5 micromolar), every 48 hours for seven days
| | Sample_growth_protocol_ch1 | Endothelial cells were grown in complete medium containing fetal calf serum at 37 degrees celcius
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on a Human U 133 plus 2.0 GeneChip Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | Gene level expression index was generated using the Affymetrix MAS 5.0 software.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Tianwei,,Yu
| | Sample_contact_department | Biostatistics
| | Sample_contact_institute | Emory University
| | Sample_contact_address | 1518 Clifton Rd NE
| | Sample_contact_city | Atlanta
| | Sample_contact_state | GA
| | Sample_contact_zip/postal_code | 30322
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM614nnn/GSM614514/suppl/GSM614514_Sample_14.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM614nnn/GSM614514/suppl/GSM614514_Sample_14.CHP.gz
| | Sample_series_id | GSE25014
| | Sample_data_row_count | 54675
| | |
|
GSM614515 | GPL570 |
|
PAECs treated with vehicle rep 3
|
cultured lung endothelial cells
|
cell type: pulmonary artery endothelial cells
treatment: control
|
Gene expression data of PAECs cultured for seven days in normal medium
|
| Sample_geo_accession | GSM614515
| | Sample_status | Public on Oct 13 2011
| | Sample_submission_date | Oct 29 2010
| | Sample_last_update_date | Oct 13 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were treated with freshly prepared heme (5 micromolar), every 48 hours for seven days
| | Sample_growth_protocol_ch1 | Endothelial cells were grown in complete medium containing fetal calf serum at 37 degrees celcius
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on a Human U 133 plus 2.0 GeneChip Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | Gene level expression index was generated using the Affymetrix MAS 5.0 software.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Tianwei,,Yu
| | Sample_contact_department | Biostatistics
| | Sample_contact_institute | Emory University
| | Sample_contact_address | 1518 Clifton Rd NE
| | Sample_contact_city | Atlanta
| | Sample_contact_state | GA
| | Sample_contact_zip/postal_code | 30322
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM614nnn/GSM614515/suppl/GSM614515_Sample_15.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM614nnn/GSM614515/suppl/GSM614515_Sample_15.CHP.gz
| | Sample_series_id | GSE25014
| | Sample_data_row_count | 54675
| | |
|
GSM614516 | GPL570 |
|
PAECs treated with vehicle rep 4
|
cultured lung endothelial cells
|
cell type: pulmonary artery endothelial cells
treatment: control
|
Gene expression data of PAECs cultured for seven days in normal medium
|
| Sample_geo_accession | GSM614516
| | Sample_status | Public on Oct 13 2011
| | Sample_submission_date | Oct 29 2010
| | Sample_last_update_date | Oct 13 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were treated with freshly prepared heme (5 micromolar), every 48 hours for seven days
| | Sample_growth_protocol_ch1 | Endothelial cells were grown in complete medium containing fetal calf serum at 37 degrees celcius
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on a Human U 133 plus 2.0 GeneChip Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | Gene level expression index was generated using the Affymetrix MAS 5.0 software.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Tianwei,,Yu
| | Sample_contact_department | Biostatistics
| | Sample_contact_institute | Emory University
| | Sample_contact_address | 1518 Clifton Rd NE
| | Sample_contact_city | Atlanta
| | Sample_contact_state | GA
| | Sample_contact_zip/postal_code | 30322
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM614nnn/GSM614516/suppl/GSM614516_Sample_16.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM614nnn/GSM614516/suppl/GSM614516_Sample_16.CHP.gz
| | Sample_series_id | GSE25014
| | Sample_data_row_count | 54675
| | |
|
GSM614517 | GPL570 |
|
PAECs treated with vehicle rep 5
|
cultured lung endothelial cells
|
cell type: pulmonary artery endothelial cells
treatment: control
|
Gene expression data of PAECs cultured for seven days in normal medium
|
| Sample_geo_accession | GSM614517
| | Sample_status | Public on Oct 13 2011
| | Sample_submission_date | Oct 29 2010
| | Sample_last_update_date | Oct 13 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were treated with freshly prepared heme (5 micromolar), every 48 hours for seven days
| | Sample_growth_protocol_ch1 | Endothelial cells were grown in complete medium containing fetal calf serum at 37 degrees celcius
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on a Human U 133 plus 2.0 GeneChip Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | Gene level expression index was generated using the Affymetrix MAS 5.0 software.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Tianwei,,Yu
| | Sample_contact_department | Biostatistics
| | Sample_contact_institute | Emory University
| | Sample_contact_address | 1518 Clifton Rd NE
| | Sample_contact_city | Atlanta
| | Sample_contact_state | GA
| | Sample_contact_zip/postal_code | 30322
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM614nnn/GSM614517/suppl/GSM614517_Sample_17.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM614nnn/GSM614517/suppl/GSM614517_Sample_17.CHP.gz
| | Sample_series_id | GSE25014
| | Sample_data_row_count | 54675
| | |
|
GSM614518 | GPL570 |
|
PAECs treated with vehicle rep 6
|
cultured lung endothelial cells
|
cell type: pulmonary artery endothelial cells
treatment: control
|
Gene expression data of PAECs cultured for seven days in normal medium
|
| Sample_geo_accession | GSM614518
| | Sample_status | Public on Oct 13 2011
| | Sample_submission_date | Oct 29 2010
| | Sample_last_update_date | Oct 13 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were treated with freshly prepared heme (5 micromolar), every 48 hours for seven days
| | Sample_growth_protocol_ch1 | Endothelial cells were grown in complete medium containing fetal calf serum at 37 degrees celcius
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on a Human U 133 plus 2.0 GeneChip Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | Gene level expression index was generated using the Affymetrix MAS 5.0 software.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Tianwei,,Yu
| | Sample_contact_department | Biostatistics
| | Sample_contact_institute | Emory University
| | Sample_contact_address | 1518 Clifton Rd NE
| | Sample_contact_city | Atlanta
| | Sample_contact_state | GA
| | Sample_contact_zip/postal_code | 30322
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM614nnn/GSM614518/suppl/GSM614518_Sample_18.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM614nnn/GSM614518/suppl/GSM614518_Sample_18.CHP.gz
| | Sample_series_id | GSE25014
| | Sample_data_row_count | 54675
| | |
|
GSM614519 | GPL570 |
|
PAECs treated with 5 micromolar heme rep 1
|
cultured lung endothelial cells
|
cell type: pulmonary artery endothelial cells
treatment: heme
|
Gene expression data of PAECs cultured for seven days in five micromolar heme
|
| Sample_geo_accession | GSM614519
| | Sample_status | Public on Oct 13 2011
| | Sample_submission_date | Oct 29 2010
| | Sample_last_update_date | Oct 13 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were treated with freshly prepared heme (5 micromolar), every 48 hours for seven days
| | Sample_growth_protocol_ch1 | Endothelial cells were grown in complete medium containing fetal calf serum at 37 degrees celcius
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on a Human U 133 plus 2.0 GeneChip Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | Gene level expression index was generated using the Affymetrix MAS 5.0 software.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Tianwei,,Yu
| | Sample_contact_department | Biostatistics
| | Sample_contact_institute | Emory University
| | Sample_contact_address | 1518 Clifton Rd NE
| | Sample_contact_city | Atlanta
| | Sample_contact_state | GA
| | Sample_contact_zip/postal_code | 30322
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM614nnn/GSM614519/suppl/GSM614519_Sample_19.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM614nnn/GSM614519/suppl/GSM614519_Sample_19.CHP.gz
| | Sample_series_id | GSE25014
| | Sample_data_row_count | 54675
| | |
|
GSM614520 | GPL570 |
|
PAECs treated with 5 micromolar heme rep 2
|
cultured lung endothelial cells
|
cell type: pulmonary artery endothelial cells
treatment: heme
|
Gene expression data of PAECs cultured for seven days in five micromolar heme
|
| Sample_geo_accession | GSM614520
| | Sample_status | Public on Oct 13 2011
| | Sample_submission_date | Oct 29 2010
| | Sample_last_update_date | Oct 13 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were treated with freshly prepared heme (5 micromolar), every 48 hours for seven days
| | Sample_growth_protocol_ch1 | Endothelial cells were grown in complete medium containing fetal calf serum at 37 degrees celcius
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on a Human U 133 plus 2.0 GeneChip Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | Gene level expression index was generated using the Affymetrix MAS 5.0 software.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Tianwei,,Yu
| | Sample_contact_department | Biostatistics
| | Sample_contact_institute | Emory University
| | Sample_contact_address | 1518 Clifton Rd NE
| | Sample_contact_city | Atlanta
| | Sample_contact_state | GA
| | Sample_contact_zip/postal_code | 30322
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM614nnn/GSM614520/suppl/GSM614520_Sample_20.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM614nnn/GSM614520/suppl/GSM614520_Sample_20.CHP.gz
| | Sample_series_id | GSE25014
| | Sample_data_row_count | 54675
| | |
|
GSM614521 | GPL570 |
|
PAECs treated with 5 micromolar heme rep 3
|
cultured lung endothelial cells
|
cell type: pulmonary artery endothelial cells
treatment: heme
|
Gene expression data of PAECs cultured for seven days in five micromolar heme
|
| Sample_geo_accession | GSM614521
| | Sample_status | Public on Oct 13 2011
| | Sample_submission_date | Oct 29 2010
| | Sample_last_update_date | Oct 13 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were treated with freshly prepared heme (5 micromolar), every 48 hours for seven days
| | Sample_growth_protocol_ch1 | Endothelial cells were grown in complete medium containing fetal calf serum at 37 degrees celcius
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on a Human U 133 plus 2.0 GeneChip Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | Gene level expression index was generated using the Affymetrix MAS 5.0 software.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Tianwei,,Yu
| | Sample_contact_department | Biostatistics
| | Sample_contact_institute | Emory University
| | Sample_contact_address | 1518 Clifton Rd NE
| | Sample_contact_city | Atlanta
| | Sample_contact_state | GA
| | Sample_contact_zip/postal_code | 30322
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM614nnn/GSM614521/suppl/GSM614521_Sample_21.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM614nnn/GSM614521/suppl/GSM614521_Sample_21.CHP.gz
| | Sample_series_id | GSE25014
| | Sample_data_row_count | 54675
| | |
|
GSM614522 | GPL570 |
|
PAECs treated with 5 micromolar heme rep 4
|
cultured lung endothelial cells
|
cell type: pulmonary artery endothelial cells
treatment: heme
|
Gene expression data of PAECs cultured for seven days in five micromolar heme
|
| Sample_geo_accession | GSM614522
| | Sample_status | Public on Oct 13 2011
| | Sample_submission_date | Oct 29 2010
| | Sample_last_update_date | Oct 13 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were treated with freshly prepared heme (5 micromolar), every 48 hours for seven days
| | Sample_growth_protocol_ch1 | Endothelial cells were grown in complete medium containing fetal calf serum at 37 degrees celcius
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on a Human U 133 plus 2.0 GeneChip Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | Gene level expression index was generated using the Affymetrix MAS 5.0 software.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Tianwei,,Yu
| | Sample_contact_department | Biostatistics
| | Sample_contact_institute | Emory University
| | Sample_contact_address | 1518 Clifton Rd NE
| | Sample_contact_city | Atlanta
| | Sample_contact_state | GA
| | Sample_contact_zip/postal_code | 30322
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM614nnn/GSM614522/suppl/GSM614522_Sample_22.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM614nnn/GSM614522/suppl/GSM614522_Sample_22.CHP.gz
| | Sample_series_id | GSE25014
| | Sample_data_row_count | 54675
| | |
|
GSM614523 | GPL570 |
|
PAECs treated with 5 micromolar heme rep 5
|
cultured lung endothelial cells
|
cell type: pulmonary artery endothelial cells
treatment: heme
|
Gene expression data of PAECs cultured for seven days in five micromolar heme
|
| Sample_geo_accession | GSM614523
| | Sample_status | Public on Oct 13 2011
| | Sample_submission_date | Oct 29 2010
| | Sample_last_update_date | Oct 13 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were treated with freshly prepared heme (5 micromolar), every 48 hours for seven days
| | Sample_growth_protocol_ch1 | Endothelial cells were grown in complete medium containing fetal calf serum at 37 degrees celcius
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on a Human U 133 plus 2.0 GeneChip Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | Gene level expression index was generated using the Affymetrix MAS 5.0 software.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Tianwei,,Yu
| | Sample_contact_department | Biostatistics
| | Sample_contact_institute | Emory University
| | Sample_contact_address | 1518 Clifton Rd NE
| | Sample_contact_city | Atlanta
| | Sample_contact_state | GA
| | Sample_contact_zip/postal_code | 30322
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM614nnn/GSM614523/suppl/GSM614523_Sample_23.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM614nnn/GSM614523/suppl/GSM614523_Sample_23.CHP.gz
| | Sample_series_id | GSE25014
| | Sample_data_row_count | 54675
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GSM614524 | GPL570 |
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PAECs treated with 5 micromolar heme rep 6
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cultured lung endothelial cells
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cell type: pulmonary artery endothelial cells
treatment: heme
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Gene expression data of PAECs cultured for seven days in five micromolar heme
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| Sample_geo_accession | GSM614524
| | Sample_status | Public on Oct 13 2011
| | Sample_submission_date | Oct 29 2010
| | Sample_last_update_date | Oct 13 2011
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were treated with freshly prepared heme (5 micromolar), every 48 hours for seven days
| | Sample_growth_protocol_ch1 | Endothelial cells were grown in complete medium containing fetal calf serum at 37 degrees celcius
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on a Human U 133 plus 2.0 GeneChip Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | Gene level expression index was generated using the Affymetrix MAS 5.0 software.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Tianwei,,Yu
| | Sample_contact_department | Biostatistics
| | Sample_contact_institute | Emory University
| | Sample_contact_address | 1518 Clifton Rd NE
| | Sample_contact_city | Atlanta
| | Sample_contact_state | GA
| | Sample_contact_zip/postal_code | 30322
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM614nnn/GSM614524/suppl/GSM614524_Sample_24.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM614nnn/GSM614524/suppl/GSM614524_Sample_24.CHP.gz
| | Sample_series_id | GSE25014
| | Sample_data_row_count | 54675
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