Result

Search results for the GEO ID: GSE25039

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GSM ID

GPL ID

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Title

Source name

Description

Characteristics

GSM614975

GPL85

Control Rat1, T0

Control Rat1 cells, at the time of serum re-addition

cell type: Fibroblasts cell line: Rat1 time: 0 min transfection: control vector

Gene expression data from Control Rat1 cells, at the time of serum re-addition

Sample_geo_accession	GSM614975
Sample_status	Public on Dec 21 2010
Sample_submission_date	Oct 29 2010
Sample_last_update_date	Dec 21 2010
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Rattus norvegicus
Sample_taxid_ch1	10116
Sample_treatment_protocol_ch1	For serum induction, cells were grown for 48 h in 0.1% serum (serum-starvation) in the presence of 4-OHT and harvested at the indicated time after switching to media with 10% serum. Total RNA was prepared at the time of serum re-addition and 90' thereafter using the TRIzol reagent (GIBCO-BRL).
Sample_growth_protocol_ch1	Cells were cultured in DMEM, supplemented with 10% foetal calf serum, at 37°C in 5% CO2.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was extracted with the TRIzol reagent (GIBCO-BRL) according to the manufacturer’s protocol. RNA was checked for quantity, purity and integrity by gel electrophoresis and UV spectrophotometric measurements.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated target RNA was prepared according to the instructions provided in the Affymetrix GeneChip Expression Analysis manual. This involved synthesis and cleanup of double-stranded cDNA followed by synthesis, cleanup and fragmentation of biotin-labeled cRNA.
Sample_hyb_protocol	Each biotin-labeled and fragmented cRNA sample was hybridized onto a GeneChip® Probe Array RG-U34A for 16 h at 45°C in a GeneChip Hybridization Oven - according to the manufacturer's recommendation.
Sample_scan_protocol	The expression probe arrays (RG-U34A) were washed and stained through a GeneChip Fluidics Station 400 according to the Affymetrix GeneChip Expression Analysis manual. The probe arrays were scanned using an HP Gene Array Scanner, controlled by the GeneChip software.
Sample_data_processing	Data were processed with the GeneChip Microarray Analysis Suite version 4.0 software (MAS 4, Affymetrix) with default analysis settings. The Rat1_control_T0 sample was taken as reference for calculating relative expression values.
Sample_platform_id	GPL85
Sample_contact_name	Sergio,,Nasi
Sample_contact_email	sergio.nasi@uniroma1.it
Sample_contact_phone	0649912241
Sample_contact_fax	0649912500
Sample_contact_department	IBPM - Sapienza Università di Roma
Sample_contact_institute	CNR - Consiglio Nazionale delle Ricerche
Sample_contact_address	P.le A. Moro 5
Sample_contact_city	Roma
Sample_contact_state	Italia
Sample_contact_zip/postal_code	00185
Sample_contact_country	Italy
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM614nnn/GSM614975/suppl/GSM614975_Rat1_Control_T0.CEL.gz
Sample_series_id	GSE25039
Sample_data_row_count	8799

GSM614976

GPL85

Omomer expressing Rat1, T0'

Omomer expressing Rat1 cells, at the time of serum re-addition

cell type: Fibroblasts cell line: Rat1 time: 0 min transfection: control vector

Gene expression data from Rat1 cells expressing Omomer, at the time of serum re-addition

Sample_geo_accession	GSM614976
Sample_status	Public on Dec 21 2010
Sample_submission_date	Oct 29 2010
Sample_last_update_date	Dec 21 2010
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Rattus norvegicus
Sample_taxid_ch1	10116
Sample_treatment_protocol_ch1	For serum induction, cells were grown for 48 h in 0.1% serum (serum-starvation) in the presence of 4-OHT and harvested at the indicated time after switching to media with 10% serum. Total RNA was prepared at the time of serum re-addition and 90' thereafter using the TRIzol reagent (GIBCO-BRL).
Sample_growth_protocol_ch1	Cells were cultured in DMEM, supplemented with 10% foetal calf serum, at 37°C in 5% CO2.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was extracted with the TRIzol reagent (GIBCO-BRL) according to the manufacturer’s protocol. RNA was checked for quantity, purity and integrity by gel electrophoresis and UV spectrophotometric measurements.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated target RNA was prepared according to the instructions provided in the Affymetrix GeneChip Expression Analysis manual. This involved synthesis and cleanup of double-stranded cDNA followed by synthesis, cleanup and fragmentation of biotin-labeled cRNA.
Sample_hyb_protocol	Each biotin-labeled and fragmented cRNA sample was hybridized onto a GeneChip® Probe Array RG-U34A for 16 h at 45°C in a GeneChip Hybridization Oven - according to the manufacturer's recommendation.
Sample_scan_protocol	The expression probe arrays (RG-U34A) were washed and stained through a GeneChip Fluidics Station 400 according to the Affymetrix GeneChip Expression Analysis manual. The probe arrays were scanned using an HP Gene Array Scanner, controlled by the GeneChip software.
Sample_data_processing	Data were processed with the GeneChip Microarray Analysis Suite version 4.0 software (MAS 4, Affymetrix) with default analysis settings. The Rat1_control_T0 sample was taken as reference for calculating relative expression values.
Sample_platform_id	GPL85
Sample_contact_name	Sergio,,Nasi
Sample_contact_email	sergio.nasi@uniroma1.it
Sample_contact_phone	0649912241
Sample_contact_fax	0649912500
Sample_contact_department	IBPM - Sapienza Università di Roma
Sample_contact_institute	CNR - Consiglio Nazionale delle Ricerche
Sample_contact_address	P.le A. Moro 5
Sample_contact_city	Roma
Sample_contact_state	Italia
Sample_contact_zip/postal_code	00185
Sample_contact_country	Italy
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM614nnn/GSM614976/suppl/GSM614976_Rat1_Omomer_T0.CEL.gz
Sample_series_id	GSE25039
Sample_data_row_count	8799

GSM614977

GPL85

Control Rat1, T90'

Control Rat1 cells, 90' after serum re-addition

cell type: Fibroblasts cell line: Rat1 time: 90 min transfection: Omomyc

Gene expression data from Control Rat1 cells, 90' after serum re-addition

Sample_geo_accession	GSM614977
Sample_status	Public on Dec 21 2010
Sample_submission_date	Oct 29 2010
Sample_last_update_date	Dec 21 2010
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Rattus norvegicus
Sample_taxid_ch1	10116
Sample_treatment_protocol_ch1	For serum induction, cells were grown for 48 h in 0.1% serum (serum-starvation) in the presence of 4-OHT and harvested at the indicated time after switching to media with 10% serum. Total RNA was prepared at the time of serum re-addition and 90' thereafter using the TRIzol reagent (GIBCO-BRL).
Sample_growth_protocol_ch1	Cells were cultured in DMEM, supplemented with 10% foetal calf serum, at 37°C in 5% CO2.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was extracted with the TRIzol reagent (GIBCO-BRL) according to the manufacturer’s protocol. RNA was checked for quantity, purity and integrity by gel electrophoresis and UV spectrophotometric measurements.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated target RNA was prepared according to the instructions provided in the Affymetrix GeneChip Expression Analysis manual. This involved synthesis and cleanup of double-stranded cDNA followed by synthesis, cleanup and fragmentation of biotin-labeled cRNA.
Sample_hyb_protocol	Each biotin-labeled and fragmented cRNA sample was hybridized onto a GeneChip® Probe Array RG-U34A for 16 h at 45°C in a GeneChip Hybridization Oven - according to the manufacturer's recommendation.
Sample_scan_protocol	The expression probe arrays (RG-U34A) were washed and stained through a GeneChip Fluidics Station 400 according to the Affymetrix GeneChip Expression Analysis manual. The probe arrays were scanned using an HP Gene Array Scanner, controlled by the GeneChip software.
Sample_data_processing	Data were processed with the GeneChip Microarray Analysis Suite version 4.0 software (MAS 4, Affymetrix) with default analysis settings. The Rat1_control_T0 sample was taken as reference for calculating relative expression values.
Sample_platform_id	GPL85
Sample_contact_name	Sergio,,Nasi
Sample_contact_email	sergio.nasi@uniroma1.it
Sample_contact_phone	0649912241
Sample_contact_fax	0649912500
Sample_contact_department	IBPM - Sapienza Università di Roma
Sample_contact_institute	CNR - Consiglio Nazionale delle Ricerche
Sample_contact_address	P.le A. Moro 5
Sample_contact_city	Roma
Sample_contact_state	Italia
Sample_contact_zip/postal_code	00185
Sample_contact_country	Italy
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM614nnn/GSM614977/suppl/GSM614977_Rat1_Control_T90_.CEL.gz
Sample_series_id	GSE25039
Sample_data_row_count	8799

GSM614978

GPL85

Omomer expressing Rat1, T90'

Omomer expressing Rat1 cells, 90' after serum re-addition

cell type: Fibroblasts cell line: Rat1 time: 90 min transfection: Omomyc

Gene expression data from Rat1 cells expressing Omomer, 90' after serum re-addition

Sample_geo_accession	GSM614978
Sample_status	Public on Dec 21 2010
Sample_submission_date	Oct 29 2010
Sample_last_update_date	Dec 21 2010
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Rattus norvegicus
Sample_taxid_ch1	10116
Sample_treatment_protocol_ch1	For serum induction, cells were grown for 48 h in 0.1% serum (serum-starvation) in the presence of 4-OHT and harvested at the indicated time after switching to media with 10% serum. Total RNA was prepared at the time of serum re-addition and 90' thereafter using the TRIzol reagent (GIBCO-BRL).
Sample_growth_protocol_ch1	Cells were cultured in DMEM, supplemented with 10% foetal calf serum, at 37°C in 5% CO2.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was extracted with the TRIzol reagent (GIBCO-BRL) according to the manufacturer’s protocol. RNA was checked for quantity, purity and integrity by gel electrophoresis and UV spectrophotometric measurements.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated target RNA was prepared according to the instructions provided in the Affymetrix GeneChip Expression Analysis manual. This involved synthesis and cleanup of double-stranded cDNA followed by synthesis, cleanup and fragmentation of biotin-labeled cRNA.
Sample_hyb_protocol	Each biotin-labeled and fragmented cRNA sample was hybridized onto a GeneChip® Probe Array RG-U34A for 16 h at 45°C in a GeneChip Hybridization Oven - according to the manufacturer's recommendation.
Sample_scan_protocol	The expression probe arrays (RG-U34A) were washed and stained through a GeneChip Fluidics Station 400 according to the Affymetrix GeneChip Expression Analysis manual. The probe arrays were scanned using an HP Gene Array Scanner, controlled by the GeneChip software.
Sample_data_processing	Data were processed with the GeneChip Microarray Analysis Suite version 4.0 software (MAS 4, Affymetrix) with default analysis settings. The Rat1_control_T0 sample was taken as reference for calculating relative expression values.
Sample_platform_id	GPL85
Sample_contact_name	Sergio,,Nasi
Sample_contact_email	sergio.nasi@uniroma1.it
Sample_contact_phone	0649912241
Sample_contact_fax	0649912500
Sample_contact_department	IBPM - Sapienza Università di Roma
Sample_contact_institute	CNR - Consiglio Nazionale delle Ricerche
Sample_contact_address	P.le A. Moro 5
Sample_contact_city	Roma
Sample_contact_state	Italia
Sample_contact_zip/postal_code	00185
Sample_contact_country	Italy
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM614nnn/GSM614978/suppl/GSM614978_Rat1_Omomer_T90_.CEL.gz
Sample_series_id	GSE25039
Sample_data_row_count	8799

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