Search results for the GEO ID: GSE25085  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM616102 | GPL570 |
|
Fetal Liver SP4 thymic implant #1
|
CD3+CD4+ Thymocytes derived from Fetal Liver HSC
|
cell type: Sort Purifed Thymocytes
original cells: fetal liver HSC
|
Gene Expression
|
| Sample_geo_accession | GSM616102
| | Sample_status | Public on Dec 17 2010
| | Sample_submission_date | Nov 02 2010
| | Sample_last_update_date | Dec 17 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | Cells were isolated from human fetal thymic implants (implanted under kidney capsule of SCID mouse) by FACS (Live Marker, HLA-A2+CD3+CD4+CD8-CD25-)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Immediately after sorting cell pellets were lysed in RNA lysis buffer (Stratagene) and RNA was obtained using the Absolutely RNA microprep kit (Stratagene) in accordance with manufacturer’s protocol
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | RNA was converted to cDNA and amplified (NuGEN WT-Ovation kit) and then fragmented (WT-Ovation kit) and labeled with biotin (FL-Ovation cDNA biotin module, NuGEN)
| | Sample_hyb_protocol | Arrays were hybridized using Affymetrix GeneChip Fluidics Station 450 according to manufacturer's protocol
| | Sample_scan_protocol | Arrays were scanned with an Affymetrix Model 3000 scanner
| | Sample_data_processing | Data were analyzed using the Bioconductor package affyPLM. Preprocessing followed the following procedures: (1) background correction; (2) normalization using the quantile method; (3) summarization of probe set values using the RMA (Robust Multi-Array Average) method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jeff,E.,Mold
| | Sample_contact_laboratory | Joseph M. McCune lab
| | Sample_contact_department | Division of Experimental Medicine
| | Sample_contact_institute | University of California San Francisco
| | Sample_contact_address | UCSF Box 1234, SFGH Bldg 3
| | Sample_contact_city | San Francisco
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 94143-1234
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM616nnn/GSM616102/suppl/GSM616102.CEL.gz
| | Sample_series_id | GSE25085
| | Sample_series_id | GSE25119
| | Sample_data_row_count | 54613
| | |
|
GSM616103 | GPL570 |
|
Fetal Liver SP4 thymic implant #2
|
CD3+CD4+ Thymocytes derived from Fetal Liver HSC
|
cell type: Sort Purifed Thymocytes
original cells: fetal liver HSC
|
Gene Expression
|
| Sample_geo_accession | GSM616103
| | Sample_status | Public on Dec 17 2010
| | Sample_submission_date | Nov 02 2010
| | Sample_last_update_date | Dec 17 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | Cells were isolated from human fetal thymic implants (implanted under kidney capsule of SCID mouse) by FACS (Live Marker, HLA-A2+CD3+CD4+CD8-CD25-)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Immediately after sorting cell pellets were lysed in RNA lysis buffer (Stratagene) and RNA was obtained using the Absolutely RNA microprep kit (Stratagene) in accordance with manufacturer’s protocol
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | RNA was converted to cDNA and amplified (NuGEN WT-Ovation kit) and then fragmented (WT-Ovation kit) and labeled with biotin (FL-Ovation cDNA biotin module, NuGEN)
| | Sample_hyb_protocol | Arrays were hybridized using Affymetrix GeneChip Fluidics Station 450 according to manufacturer's protocol
| | Sample_scan_protocol | Arrays were scanned with an Affymetrix Model 3000 scanner
| | Sample_data_processing | Data were analyzed using the Bioconductor package affyPLM. Preprocessing followed the following procedures: (1) background correction; (2) normalization using the quantile method; (3) summarization of probe set values using the RMA (Robust Multi-Array Average) method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jeff,E.,Mold
| | Sample_contact_laboratory | Joseph M. McCune lab
| | Sample_contact_department | Division of Experimental Medicine
| | Sample_contact_institute | University of California San Francisco
| | Sample_contact_address | UCSF Box 1234, SFGH Bldg 3
| | Sample_contact_city | San Francisco
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 94143-1234
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM616nnn/GSM616103/suppl/GSM616103.CEL.gz
| | Sample_series_id | GSE25085
| | Sample_series_id | GSE25119
| | Sample_data_row_count | 54613
| | |
|
GSM616104 | GPL570 |
|
Fetal Liver SP4 thymic implant #3
|
CD3+CD4+ Thymocytes derived from Fetal Liver HSC
|
cell type: Sort Purifed Thymocytes
original cells: fetal liver HSC
|
Gene Expression
|
| Sample_geo_accession | GSM616104
| | Sample_status | Public on Dec 17 2010
| | Sample_submission_date | Nov 02 2010
| | Sample_last_update_date | Dec 17 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | Cells were isolated from human fetal thymic implants (implanted under kidney capsule of SCID mouse) by FACS (Live Marker, HLA-A2+CD3+CD4+CD8-CD25-)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Immediately after sorting cell pellets were lysed in RNA lysis buffer (Stratagene) and RNA was obtained using the Absolutely RNA microprep kit (Stratagene) in accordance with manufacturer’s protocol
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | RNA was converted to cDNA and amplified (NuGEN WT-Ovation kit) and then fragmented (WT-Ovation kit) and labeled with biotin (FL-Ovation cDNA biotin module, NuGEN)
| | Sample_hyb_protocol | Arrays were hybridized using Affymetrix GeneChip Fluidics Station 450 according to manufacturer's protocol
| | Sample_scan_protocol | Arrays were scanned with an Affymetrix Model 3000 scanner
| | Sample_data_processing | Data were analyzed using the Bioconductor package affyPLM. Preprocessing followed the following procedures: (1) background correction; (2) normalization using the quantile method; (3) summarization of probe set values using the RMA (Robust Multi-Array Average) method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jeff,E.,Mold
| | Sample_contact_laboratory | Joseph M. McCune lab
| | Sample_contact_department | Division of Experimental Medicine
| | Sample_contact_institute | University of California San Francisco
| | Sample_contact_address | UCSF Box 1234, SFGH Bldg 3
| | Sample_contact_city | San Francisco
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 94143-1234
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM616nnn/GSM616104/suppl/GSM616104.CEL.gz
| | Sample_series_id | GSE25085
| | Sample_series_id | GSE25119
| | Sample_data_row_count | 54613
| | |
|
GSM616105 | GPL570 |
|
Fetal BM SP4 thymic implant #1
|
CD3+CD4+ Thymocytes derived from Fetal BM HSC
|
cell type: Sort Purifed Thymocytes
original cells: fetal bone marrow HSC
|
Gene Expression
|
| Sample_geo_accession | GSM616105
| | Sample_status | Public on Dec 17 2010
| | Sample_submission_date | Nov 02 2010
| | Sample_last_update_date | Dec 17 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | Cells were isolated from human fetal thymic implants (implanted under kidney capsule of SCID mouse) by FACS (Live Marker, HLA-A2+CD3+CD4+CD8-CD25-)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Immediately after sorting cell pellets were lysed in RNA lysis buffer (Stratagene) and RNA was obtained using the Absolutely RNA microprep kit (Stratagene) in accordance with manufacturer’s protocol
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | RNA was converted to cDNA and amplified (NuGEN WT-Ovation kit) and then fragmented (WT-Ovation kit) and labeled with biotin (FL-Ovation cDNA biotin module, NuGEN)
| | Sample_hyb_protocol | Arrays were hybridized using Affymetrix GeneChip Fluidics Station 450 according to manufacturer's protocol
| | Sample_scan_protocol | Arrays were scanned with an Affymetrix Model 3000 scanner
| | Sample_data_processing | Data were analyzed using the Bioconductor package affyPLM. Preprocessing followed the following procedures: (1) background correction; (2) normalization using the quantile method; (3) summarization of probe set values using the RMA (Robust Multi-Array Average) method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jeff,E.,Mold
| | Sample_contact_laboratory | Joseph M. McCune lab
| | Sample_contact_department | Division of Experimental Medicine
| | Sample_contact_institute | University of California San Francisco
| | Sample_contact_address | UCSF Box 1234, SFGH Bldg 3
| | Sample_contact_city | San Francisco
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 94143-1234
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM616nnn/GSM616105/suppl/GSM616105.CEL.gz
| | Sample_series_id | GSE25085
| | Sample_series_id | GSE25119
| | Sample_data_row_count | 54613
| | |
|
GSM616106 | GPL570 |
|
Fetal BM SP4 thymic implant #2
|
CD3+CD4+ Thymocytes derived from Fetal BM HSC
|
cell type: Sort Purifed Thymocytes
original cells: fetal bone marrow HSC
|
Gene Expression
|
| Sample_geo_accession | GSM616106
| | Sample_status | Public on Dec 17 2010
| | Sample_submission_date | Nov 02 2010
| | Sample_last_update_date | Dec 17 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | Cells were isolated from human fetal thymic implants (implanted under kidney capsule of SCID mouse) by FACS (Live Marker, HLA-A2+CD3+CD4+CD8-CD25-)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Immediately after sorting cell pellets were lysed in RNA lysis buffer (Stratagene) and RNA was obtained using the Absolutely RNA microprep kit (Stratagene) in accordance with manufacturer’s protocol
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | RNA was converted to cDNA and amplified (NuGEN WT-Ovation kit) and then fragmented (WT-Ovation kit) and labeled with biotin (FL-Ovation cDNA biotin module, NuGEN)
| | Sample_hyb_protocol | Arrays were hybridized using Affymetrix GeneChip Fluidics Station 450 according to manufacturer's protocol
| | Sample_scan_protocol | Arrays were scanned with an Affymetrix Model 3000 scanner
| | Sample_data_processing | Data were analyzed using the Bioconductor package affyPLM. Preprocessing followed the following procedures: (1) background correction; (2) normalization using the quantile method; (3) summarization of probe set values using the RMA (Robust Multi-Array Average) method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jeff,E.,Mold
| | Sample_contact_laboratory | Joseph M. McCune lab
| | Sample_contact_department | Division of Experimental Medicine
| | Sample_contact_institute | University of California San Francisco
| | Sample_contact_address | UCSF Box 1234, SFGH Bldg 3
| | Sample_contact_city | San Francisco
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 94143-1234
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM616nnn/GSM616106/suppl/GSM616106.CEL.gz
| | Sample_series_id | GSE25085
| | Sample_series_id | GSE25119
| | Sample_data_row_count | 54613
| | |
|
GSM616107 | GPL570 |
|
Fetal BM SP4 thymic implant #3
|
CD3+CD4+ Thymocytes derived from Fetal BM HSC
|
cell type: Sort Purifed Thymocytes
original cells: fetal bone marrow HSC
|
Gene Expression
|
| Sample_geo_accession | GSM616107
| | Sample_status | Public on Dec 17 2010
| | Sample_submission_date | Nov 02 2010
| | Sample_last_update_date | Dec 17 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | Cells were isolated from human fetal thymic implants (implanted under kidney capsule of SCID mouse) by FACS (Live Marker, HLA-A2+CD3+CD4+CD8-CD25-)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Immediately after sorting cell pellets were lysed in RNA lysis buffer (Stratagene) and RNA was obtained using the Absolutely RNA microprep kit (Stratagene) in accordance with manufacturer’s protocol
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | RNA was converted to cDNA and amplified (NuGEN WT-Ovation kit) and then fragmented (WT-Ovation kit) and labeled with biotin (FL-Ovation cDNA biotin module, NuGEN)
| | Sample_hyb_protocol | Arrays were hybridized using Affymetrix GeneChip Fluidics Station 450 according to manufacturer's protocol
| | Sample_scan_protocol | Arrays were scanned with an Affymetrix Model 3000 scanner
| | Sample_data_processing | Data were analyzed using the Bioconductor package affyPLM. Preprocessing followed the following procedures: (1) background correction; (2) normalization using the quantile method; (3) summarization of probe set values using the RMA (Robust Multi-Array Average) method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jeff,E.,Mold
| | Sample_contact_laboratory | Joseph M. McCune lab
| | Sample_contact_department | Division of Experimental Medicine
| | Sample_contact_institute | University of California San Francisco
| | Sample_contact_address | UCSF Box 1234, SFGH Bldg 3
| | Sample_contact_city | San Francisco
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 94143-1234
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM616nnn/GSM616107/suppl/GSM616107.CEL.gz
| | Sample_series_id | GSE25085
| | Sample_series_id | GSE25119
| | Sample_data_row_count | 54613
| | |
|
GSM616108 | GPL570 |
|
Adult BM SP4 thymic implant #1
|
CD3+CD4+ Thymocytes derived from Adult BM HSC
|
cell type: Sort Purifed Thymocytes
original cells: adult bone marrow HSC
|
Gene Expression
|
| Sample_geo_accession | GSM616108
| | Sample_status | Public on Dec 17 2010
| | Sample_submission_date | Nov 02 2010
| | Sample_last_update_date | Dec 17 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | Cells were isolated from human fetal thymic implants (implanted under kidney capsule of SCID mouse) by FACS (Live Marker, HLA-A2+CD3+CD4+CD8-CD25-)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Immediately after sorting cell pellets were lysed in RNA lysis buffer (Stratagene) and RNA was obtained using the Absolutely RNA microprep kit (Stratagene) in accordance with manufacturer’s protocol
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | RNA was converted to cDNA and amplified (NuGEN WT-Ovation kit) and then fragmented (WT-Ovation kit) and labeled with biotin (FL-Ovation cDNA biotin module, NuGEN)
| | Sample_hyb_protocol | Arrays were hybridized using Affymetrix GeneChip Fluidics Station 450 according to manufacturer's protocol
| | Sample_scan_protocol | Arrays were scanned with an Affymetrix Model 3000 scanner
| | Sample_data_processing | Data were analyzed using the Bioconductor package affyPLM. Preprocessing followed the following procedures: (1) background correction; (2) normalization using the quantile method; (3) summarization of probe set values using the RMA (Robust Multi-Array Average) method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jeff,E.,Mold
| | Sample_contact_laboratory | Joseph M. McCune lab
| | Sample_contact_department | Division of Experimental Medicine
| | Sample_contact_institute | University of California San Francisco
| | Sample_contact_address | UCSF Box 1234, SFGH Bldg 3
| | Sample_contact_city | San Francisco
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 94143-1234
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM616nnn/GSM616108/suppl/GSM616108.CEL.gz
| | Sample_series_id | GSE25085
| | Sample_series_id | GSE25119
| | Sample_data_row_count | 54613
| | |
|
GSM616109 | GPL570 |
|
Adult BM SP4 thymic implant #2
|
CD3+CD4+ Thymocytes derived from Adult BM HSC
|
cell type: Sort Purifed Thymocytes
original cells: adult bone marrow HSC
|
Gene Expression
|
| Sample_geo_accession | GSM616109
| | Sample_status | Public on Dec 17 2010
| | Sample_submission_date | Nov 02 2010
| | Sample_last_update_date | Dec 17 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | Cells were isolated from human fetal thymic implants (implanted under kidney capsule of SCID mouse) by FACS (Live Marker, HLA-A2+CD3+CD4+CD8-CD25-)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Immediately after sorting cell pellets were lysed in RNA lysis buffer (Stratagene) and RNA was obtained using the Absolutely RNA microprep kit (Stratagene) in accordance with manufacturer’s protocol
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | RNA was converted to cDNA and amplified (NuGEN WT-Ovation kit) and then fragmented (WT-Ovation kit) and labeled with biotin (FL-Ovation cDNA biotin module, NuGEN)
| | Sample_hyb_protocol | Arrays were hybridized using Affymetrix GeneChip Fluidics Station 450 according to manufacturer's protocol
| | Sample_scan_protocol | Arrays were scanned with an Affymetrix Model 3000 scanner
| | Sample_data_processing | Data were analyzed using the Bioconductor package affyPLM. Preprocessing followed the following procedures: (1) background correction; (2) normalization using the quantile method; (3) summarization of probe set values using the RMA (Robust Multi-Array Average) method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jeff,E.,Mold
| | Sample_contact_laboratory | Joseph M. McCune lab
| | Sample_contact_department | Division of Experimental Medicine
| | Sample_contact_institute | University of California San Francisco
| | Sample_contact_address | UCSF Box 1234, SFGH Bldg 3
| | Sample_contact_city | San Francisco
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 94143-1234
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM616nnn/GSM616109/suppl/GSM616109.CEL.gz
| | Sample_series_id | GSE25085
| | Sample_series_id | GSE25119
| | Sample_data_row_count | 54613
| | |
|
GSM616110 | GPL570 |
|
Adult BM SP4 thymic implant #3
|
CD3+CD4+ Thymocytes derived from Adult BM HSC
|
cell type: Sort Purifed Thymocytes
original cells: adult bone marrow HSC
|
Gene Expression
|
| Sample_geo_accession | GSM616110
| | Sample_status | Public on Dec 17 2010
| | Sample_submission_date | Nov 02 2010
| | Sample_last_update_date | Dec 17 2010
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | Cells were isolated from human fetal thymic implants (implanted under kidney capsule of SCID mouse) by FACS (Live Marker, HLA-A2+CD3+CD4+CD8-CD25-)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Immediately after sorting cell pellets were lysed in RNA lysis buffer (Stratagene) and RNA was obtained using the Absolutely RNA microprep kit (Stratagene) in accordance with manufacturer’s protocol
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | RNA was converted to cDNA and amplified (NuGEN WT-Ovation kit) and then fragmented (WT-Ovation kit) and labeled with biotin (FL-Ovation cDNA biotin module, NuGEN)
| | Sample_hyb_protocol | Arrays were hybridized using Affymetrix GeneChip Fluidics Station 450 according to manufacturer's protocol
| | Sample_scan_protocol | Arrays were scanned with an Affymetrix Model 3000 scanner
| | Sample_data_processing | Data were analyzed using the Bioconductor package affyPLM. Preprocessing followed the following procedures: (1) background correction; (2) normalization using the quantile method; (3) summarization of probe set values using the RMA (Robust Multi-Array Average) method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Jeff,E.,Mold
| | Sample_contact_laboratory | Joseph M. McCune lab
| | Sample_contact_department | Division of Experimental Medicine
| | Sample_contact_institute | University of California San Francisco
| | Sample_contact_address | UCSF Box 1234, SFGH Bldg 3
| | Sample_contact_city | San Francisco
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 94143-1234
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM616nnn/GSM616110/suppl/GSM616110.CEL.gz
| | Sample_series_id | GSE25085
| | Sample_series_id | GSE25119
| | Sample_data_row_count | 54613
| | |
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