Search results for the GEO ID: GSE25090 |
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GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM616245 | GPL570 |
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CD34+ cells
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CD34 positive cells from cord blood cells
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cell type: CD34 positive cells from cord blood cells
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Gene expression data from cells
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Sample_geo_accession | GSM616245
| Sample_status | Public on Nov 03 2010
| Sample_submission_date | Nov 02 2010
| Sample_last_update_date | Nov 02 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | hES and iPS cells with a cell scraper and break up the clonies, add 10mL human ES medium. Centrifuge at 170G for 5min and discard the supernatant and then wash the cell clump twice with PBS. After centrifuge, remove PBS completely.
| Sample_growth_protocol_ch1 | In hematopoietic medium (X-Vivo10 containing 50 ng/mL IL-6, 50 ng/mL soluble IL-6 R, 50 ng/mL SCF, 10 ng/mL TPO, and 20 ng/mL Flt3-ligand)
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted with the RNeasy Mini kit, according to manufacturer’s instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared using GeneChip 3’ IVT Express Kit according to manufacturer’s instructions from 250 ng total RNA.
| Sample_hyb_protocol | Following fragmentation, cRNA were hybridized for 16 hr at 45oC, 60 rpm on Gene Chip Human Genome U133 Plus2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using GeneChip Scanner 3000 7G.
| Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| Sample_platform_id | GPL570
| Sample_contact_name | Shin,,Kawamata
| Sample_contact_department | The Basic Reserch Group for Regenerative Medicine
| Sample_contact_institute | Foundation for Biomedical and Innovation
| Sample_contact_address | TRI#308,1-5-4,Minatojima-minamimachi,Chuo-ku
| Sample_contact_city | Kobe
| Sample_contact_state | Hyogo
| Sample_contact_zip/postal_code | 650-0047
| Sample_contact_country | Japan
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM616nnn/GSM616245/suppl/GSM616245.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM616nnn/GSM616245/suppl/GSM616245.CHP.gz
| Sample_series_id | GSE25090
| Sample_data_row_count | 54675
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GSM616246 | GPL570 |
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iPS_4A cells
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human iPS cells
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cell type: human iPS cells
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Gene expression data from cells
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Sample_geo_accession | GSM616246
| Sample_status | Public on Nov 03 2010
| Sample_submission_date | Nov 02 2010
| Sample_last_update_date | Nov 02 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | hES and iPS cells with a cell scraper and break up the clonies, add 10mL human ES medium. Centrifuge at 170G for 5min and discard the supernatant and then wash the cell clump twice with PBS. After centrifuge, remove PBS completely.
| Sample_growth_protocol_ch1 | On SNL76/7 feeder cells in human embryonic stem cell medium (DMEM/F-12 containing 20% KSR, 2mM L-glutamine, 1% NEAA, 0.1 mM 2-ME, and 4 ng/mL bFGF
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted with the RNeasy Mini kit, according to manufacturer’s instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared using GeneChip 3’ IVT Express Kit according to manufacturer’s instructions from 250 ng total RNA.
| Sample_hyb_protocol | Following fragmentation, cRNA were hybridized for 16 hr at 45oC, 60 rpm on Gene Chip Human Genome U133 Plus2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using GeneChip Scanner 3000 7G.
| Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| Sample_platform_id | GPL570
| Sample_contact_name | Shin,,Kawamata
| Sample_contact_department | The Basic Reserch Group for Regenerative Medicine
| Sample_contact_institute | Foundation for Biomedical and Innovation
| Sample_contact_address | TRI#308,1-5-4,Minatojima-minamimachi,Chuo-ku
| Sample_contact_city | Kobe
| Sample_contact_state | Hyogo
| Sample_contact_zip/postal_code | 650-0047
| Sample_contact_country | Japan
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM616nnn/GSM616246/suppl/GSM616246.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM616nnn/GSM616246/suppl/GSM616246.CHP.gz
| Sample_series_id | GSE25090
| Sample_data_row_count | 54675
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GSM616247 | GPL570 |
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hES_03 cells
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human ES cells
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cell type: human ES cells
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Gene expression data from cells
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Sample_geo_accession | GSM616247
| Sample_status | Public on Nov 03 2010
| Sample_submission_date | Nov 02 2010
| Sample_last_update_date | Nov 02 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | hES and iPS cells with a cell scraper and break up the clonies, add 10mL human ES medium. Centrifuge at 170G for 5min and discard the supernatant and then wash the cell clump twice with PBS. After centrifuge, remove PBS completely.
| Sample_growth_protocol_ch1 | On SNL76/7 feeder cells in human embryonic stem cell medium (DMEM/F-12 containing 20% KSR, 2mM L-glutamine, 1% NEAA, 0.1 mM 2-ME, and 4 ng/mL bFGF
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted with the RNeasy Mini kit, according to manufacturer’s instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared using GeneChip 3’ IVT Express Kit according to manufacturer’s instructions from 250 ng total RNA.
| Sample_hyb_protocol | Following fragmentation, cRNA were hybridized for 16 hr at 45oC, 60 rpm on Gene Chip Human Genome U133 Plus2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using GeneChip Scanner 3000 7G.
| Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| Sample_platform_id | GPL570
| Sample_contact_name | Shin,,Kawamata
| Sample_contact_department | The Basic Reserch Group for Regenerative Medicine
| Sample_contact_institute | Foundation for Biomedical and Innovation
| Sample_contact_address | TRI#308,1-5-4,Minatojima-minamimachi,Chuo-ku
| Sample_contact_city | Kobe
| Sample_contact_state | Hyogo
| Sample_contact_zip/postal_code | 650-0047
| Sample_contact_country | Japan
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM616nnn/GSM616247/suppl/GSM616247.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM616nnn/GSM616247/suppl/GSM616247.CHP.gz
| Sample_series_id | GSE25090
| Sample_data_row_count | 54675
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