Search results for the GEO ID: GSE25146 |
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GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM618009 | GPL570 |
|
AGS untreated rep 1
|
AGS cells untreated for 8h rep 1
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cell type: gastric epithelial cells
cell line: AGS
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gene expression in untreated AGS cells
|
Sample_geo_accession | GSM618009
| Sample_status | Public on Jan 12 2011
| Sample_submission_date | Nov 04 2010
| Sample_last_update_date | Jan 13 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | The morning of treatment, the cell culture medium was replaced with either fresh medium (untreated) or medium containing 10 μg/ml H. pylori lipopolysaccharide
| Sample_growth_protocol_ch1 | AGS cells were routinely maintained in nutrient mixture F-12 Ham (Sigma) supplemented with 10% foetal calf serum (Gibco), 2 mM L-glutamine (Sigma), 100 U/ml penicillin (Sigma) and 100 μg/ml streptomycin. The day before treatment, cells were seeded in 6-well plates at 1x106 cells/well.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | 8h post-stimulation, cells were harvested and RNA isolated using the Nucleospin RNAII kit (Machery Nagel) according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 μg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix) using the Affymetrix IVT labeling kit.
| Sample_hyb_protocol | Following fragmentation, cRNA was hybridized for 16 hr at 45C on Affymetrix U133 plus 2.0 genechips. GeneChips were washed and stained in the Automated fluidics 450 station.
| Sample_scan_protocol | Genechips were scanned using the GeneChip 3000 7G scanner
| Sample_data_processing | Scanned images, obtained using Affymetrix Software (MAS5), were normalized using robust multichip average (RMA) analysis with RMAExpress version 0.4.1.
| Sample_platform_id | GPL570
| Sample_contact_name | Sinead,Marian,Smith
| Sample_contact_laboratory | Trinity Centre for Health Sciences
| Sample_contact_institute | Trinity College Dublin
| Sample_contact_address | St. James's Hospital
| Sample_contact_city | Dublin 8
| Sample_contact_zip/postal_code | D8
| Sample_contact_country | Ireland
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM618nnn/GSM618009/suppl/GSM618009.CEL.gz
| Sample_series_id | GSE25146
| Sample_series_id | GSE25155
| Sample_data_row_count | 54675
| |
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GSM618010 | GPL570 |
|
AGS untreated rep 2
|
AGS cells untreated for 8h rep 2
|
cell type: gastric epithelial cells
cell line: AGS
|
gene expression in untreated AGS cells
|
Sample_geo_accession | GSM618010
| Sample_status | Public on Jan 12 2011
| Sample_submission_date | Nov 04 2010
| Sample_last_update_date | Jan 13 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | The morning of treatment, the cell culture medium was replaced with either fresh medium (untreated) or medium containing 10 μg/ml H. pylori lipopolysaccharide
| Sample_growth_protocol_ch1 | AGS cells were routinely maintained in nutrient mixture F-12 Ham (Sigma) supplemented with 10% foetal calf serum (Gibco), 2 mM L-glutamine (Sigma), 100 U/ml penicillin (Sigma) and 100 μg/ml streptomycin. The day before treatment, cells were seeded in 6-well plates at 1x106 cells/well.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | 8h post-stimulation, cells were harvested and RNA isolated using the Nucleospin RNAII kit (Machery Nagel) according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 μg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix) using the Affymetrix IVT labeling kit.
| Sample_hyb_protocol | Following fragmentation, cRNA was hybridized for 16 hr at 45C on Affymetrix U133 plus 2.0 genechips. GeneChips were washed and stained in the Automated fluidics 450 station.
| Sample_scan_protocol | Genechips were scanned using the GeneChip 3000 7G scanner
| Sample_data_processing | Scanned images, obtained using Affymetrix Software (MAS5), were normalized using robust multichip average (RMA) analysis with RMAExpress version 0.4.1.
| Sample_platform_id | GPL570
| Sample_contact_name | Sinead,Marian,Smith
| Sample_contact_laboratory | Trinity Centre for Health Sciences
| Sample_contact_institute | Trinity College Dublin
| Sample_contact_address | St. James's Hospital
| Sample_contact_city | Dublin 8
| Sample_contact_zip/postal_code | D8
| Sample_contact_country | Ireland
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM618nnn/GSM618010/suppl/GSM618010.CEL.gz
| Sample_series_id | GSE25146
| Sample_series_id | GSE25155
| Sample_data_row_count | 54675
| |
|
GSM618011 | GPL570 |
|
AGS treated rep 1
|
AGS cells treated with 10 μg/ml H. pylori LPS for 8h rep 1
|
cell type: gastric epithelial cells
cell line: AGS
|
gene expression in H. pylori LPS-treated cells
|
Sample_geo_accession | GSM618011
| Sample_status | Public on Jan 12 2011
| Sample_submission_date | Nov 04 2010
| Sample_last_update_date | Jan 13 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | The morning of treatment, the cell culture medium was replaced with either fresh medium (untreated) or medium containing 10 μg/ml H. pylori lipopolysaccharide
| Sample_growth_protocol_ch1 | AGS cells were routinely maintained in nutrient mixture F-12 Ham (Sigma) supplemented with 10% foetal calf serum (Gibco), 2 mM L-glutamine (Sigma), 100 U/ml penicillin (Sigma) and 100 μg/ml streptomycin. The day before treatment, cells were seeded in 6-well plates at 1x106 cells/well.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | 8h post-stimulation, cells were harvested and RNA isolated using the Nucleospin RNAII kit (Machery Nagel) according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 μg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix) using the Affymetrix IVT labeling kit.
| Sample_hyb_protocol | Following fragmentation, cRNA was hybridized for 16 hr at 45C on Affymetrix U133 plus 2.0 genechips. GeneChips were washed and stained in the Automated fluidics 450 station.
| Sample_scan_protocol | Genechips were scanned using the GeneChip 3000 7G scanner
| Sample_data_processing | Scanned images, obtained using Affymetrix Software (MAS5), were normalized using robust multichip average (RMA) analysis with RMAExpress version 0.4.1.
| Sample_platform_id | GPL570
| Sample_contact_name | Sinead,Marian,Smith
| Sample_contact_laboratory | Trinity Centre for Health Sciences
| Sample_contact_institute | Trinity College Dublin
| Sample_contact_address | St. James's Hospital
| Sample_contact_city | Dublin 8
| Sample_contact_zip/postal_code | D8
| Sample_contact_country | Ireland
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM618nnn/GSM618011/suppl/GSM618011.CEL.gz
| Sample_series_id | GSE25146
| Sample_series_id | GSE25155
| Sample_data_row_count | 54675
| |
|
GSM618012 | GPL570 |
|
AGS treated rep 2
|
AGS cells treated with 10 μg/ml H. pylori LPS for 8h rep 2
|
cell type: gastric epithelial cells
cell line: AGS
|
gene expression in H. pylori LPS-treated cells
|
Sample_geo_accession | GSM618012
| Sample_status | Public on Jan 12 2011
| Sample_submission_date | Nov 04 2010
| Sample_last_update_date | Jan 13 2011
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | The morning of treatment, the cell culture medium was replaced with either fresh medium (untreated) or medium containing 10 μg/ml H. pylori lipopolysaccharide
| Sample_growth_protocol_ch1 | AGS cells were routinely maintained in nutrient mixture F-12 Ham (Sigma) supplemented with 10% foetal calf serum (Gibco), 2 mM L-glutamine (Sigma), 100 U/ml penicillin (Sigma) and 100 μg/ml streptomycin. The day before treatment, cells were seeded in 6-well plates at 1x106 cells/well.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | 8h post-stimulation, cells were harvested and RNA isolated using the Nucleospin RNAII kit (Machery Nagel) according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 μg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix) using the Affymetrix IVT labeling kit.
| Sample_hyb_protocol | Following fragmentation, cRNA was hybridized for 16 hr at 45C on Affymetrix U133 plus 2.0 genechips. GeneChips were washed and stained in the Automated fluidics 450 station.
| Sample_scan_protocol | Genechips were scanned using the GeneChip 3000 7G scanner
| Sample_data_processing | Scanned images, obtained using Affymetrix Software (MAS5), were normalized using robust multichip average (RMA) analysis with RMAExpress version 0.4.1.
| Sample_platform_id | GPL570
| Sample_contact_name | Sinead,Marian,Smith
| Sample_contact_laboratory | Trinity Centre for Health Sciences
| Sample_contact_institute | Trinity College Dublin
| Sample_contact_address | St. James's Hospital
| Sample_contact_city | Dublin 8
| Sample_contact_zip/postal_code | D8
| Sample_contact_country | Ireland
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM618nnn/GSM618012/suppl/GSM618012.CEL.gz
| Sample_series_id | GSE25146
| Sample_series_id | GSE25155
| Sample_data_row_count | 54675
| |
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