Sample_geo_accession | GSM618135
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Sample_status | Public on May 01 2011
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Sample_submission_date | Nov 05 2010
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Sample_last_update_date | May 01 2011
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Sample_type | RNA
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Sample_channel_count | 1
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Sample_organism_ch1 | Homo sapiens
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Sample_taxid_ch1 | 9606
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Sample_treatment_protocol_ch1 | We transfected HCV Replicon containing Huh7-K2040 cells with pTracer-CREB3L1 or pTracer. After overnight incubation, we isolated cells by trypsinization and utilized FACS to isolate GFP(+) cells.
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Sample_growth_protocol_ch1 | Cells were maintianed in DMEM (High Glucose) supplemented with 10% Fetal Calf Serum and 1% Pennicillin/Streptomycin.
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Sample_molecule_ch1 | total RNA
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Sample_extract_protocol_ch1 | Total RNA was extracted with Qiagen Rneasy Mini Kit according to the manufacturer's instructions.
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Sample_label_ch1 | Biotin
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Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
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Sample_hyb_protocol | Following fragmentation, 5 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix GeneChip U133 Plus 2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
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Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000.
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Sample_data_processing | The data were analyzed with GeneChip Operating Software, version 1.0.0.046 using Affymetrix default analysis settings (MAS 5.0 algorithm) and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
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Sample_platform_id | GPL570
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Sample_contact_name | Bray,,Denard
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Sample_contact_laboratory | Jin Ye Lab
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Sample_contact_department | Molecular Genetics
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Sample_contact_institute | University of Texas Southwestern Medical Center
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Sample_contact_address | 5323 Harry Hines Blvd. L5.226
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Sample_contact_city | Dallas
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Sample_contact_state | TX
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Sample_contact_zip/postal_code | 75390
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Sample_contact_country | USA
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Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM618nnn/GSM618135/suppl/GSM618135.CEL.gz
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Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM618nnn/GSM618135/suppl/GSM618135.CHP.gz
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Sample_series_id | GSE25157
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Sample_data_row_count | 54675
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